Publishing type：Research paper (scientific journal)  

On-site monitoring of plasma drug concentrations is required for effective therapies. Recently developed handy biosensors are not yet popular owing to insufficient evaluation of accuracy on clinical samples and the necessity of complicated costly fabrication processes. Here, we approached these bottlenecks via a strategy involving engineeringly unmodified boron-doped diamond (BDD), a sustainable electrochemical material. A sensing system based on a ∼1 cm2 BDD chip, when analysing rat plasma spiked with a molecular-targeting anticancer drug, pazopanib, detected clinically relevant concentrations. The response was stable in 60 sequential measurements on the same chip. In a clinical study, data obtained with a BDD chip were consistent with liquid chromatography–mass spectrometry results. Finally, the portable system with a palm-sized sensor containing the chip analysed ∼40 μL of whole blood from dosed rats within ∼10 min. This approach with the ‘reusable’ sensor may improve point-of-monitoring systems and personalised medicine while reducing medical costs.

DOI： 10.1016/j.heliyon.2023.e15963

Scopus

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Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

Hearing loss affects >5% of the global population and therefore, has a great social and clinical impact. Sensorineural hearing loss, which can be caused by different factors, such as acoustic trauma, aging, and administration of certain classes of drugs, stems primarily from a dysfunction of the cochlea in the inner ear. Few therapeutic strategies against sensorineural hearing loss are available. To develop effective treatments for this disease, it is crucial to precisely determine the behavior of ototoxic and therapeutic agents in the microenvironment of the cochlea in live animals. Since the 1980s, a number of studies have addressed this issue by different methodologies. However, there is much less information on pharmacokinetics in the cochlea than that in other organs; the delay in ontological pharmacology is likely due to technical difficulties with accessing the cochlea, a tiny organ that is encased with a bony wall and has a fine and complicated internal structure. In this review, we not only summarize the observations and insights obtained in classic and recent studies on pharmacokinetics in the cochlea but also describe relevant analytical techniques, with their strengths, limitations, and prospects.

DOI： 10.3389/fphar.2021.633505

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Pentraxins belong to the superfamily of conserved proteins that are characterized by a cyclic multimeric structure. Pentraxin 3 (PTX3) is a long pentraxin which can be produced by different cell types upon exposure to various inflammatory signals. Inside the neutrophil PTX3 is stored in form of granules localized in the cytoplasm. Neutrophilic granules are divided into three types: azurophilic (primary) granules, specific (secondary) granules and gelatinase (tertiary) granules. PTX3 has been considered to be localized in specific (secondary) granules. Immunofluorescent analyses using confocal laser microscopic examination were performed to clarify the localization of all three groups of granules within the cytoplasm of the mature neutrophils and neutrophils stimulated with IL-8. Furthermore, PTX3 was localized in primary granules of promyelocyte cell line HL-60. As a result, we suggest that PTX3 is localized not only in specific granules, but is also partly expressed in primary and tertiary granules. After the stimulation with IL-8, irregular reticular structures called neutrophil extracellular traps (NETs) were formed, three types of granules were trapped by NETs and PTX3 showed partial colocalization with these granular components. PTX3 localized in all three types of granules in neutrophils may play important roles in host defense. (C) 2014 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.yexmp.2014.11.009

Web of Science

PubMed

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Language：English   Publisher：ELSEVIER SCIENCE BV  

Methicillin-resistant Staphylococcus aureus (MRSA) is able to persist not only in hospitals (with a high level of antimicrobial agent use) but also in the community (with a low level of antimicrobial agent use). The former is called hospital-acquired MRSA (HA-MRSA) and the latter community-acquired MRSA (CA-MRSA). It is believed MRSA clones are generated from S. aureus through insertion of the staphylococcal cassette chromosome mec (SCCmec), and outbreaks occur as they spread. Several worldwide and regional clones have been identified, and their epidemiological, clinical, and genetic characteristics have been described. CA-MRSA is likely able to survive in the community because of suitable SCCmec types (type IV or V), a clone-specific colonization/infection nature, toxin profiles (including Pantone-Valentine leucocidin, PVL), and narrow drug resistance patterns. CA-MRSA infections are generally seen in healthy children or young athletes, with unexpected cases of diseases, and also in elderly inpatients, occasionally surprising clinicians used to HA-MRSA infections. CA-MRSA spreads within families and close-contact groups or even through public transport, demonstrating transmission cores. Re-infection (including multifocal infection) frequently occurs, if the cores are not sought out and properly eradicated. Recently, attention has been given to CA-MRSA (USA300), which originated in the US, and is growing as HA-MRSA and also as a worldwide clone. CA-MRSA infection in influenza season has increasingly been noted as well. MRSA is also found in farm and companion animals, and has occasionally transferred to humans. As such, the epidemiological, clinical, and genetic behavior of CA-MRSA, a growing threat, is focused on in this study.

DOI： 10.1007/s10156-010-0045-9

Web of Science

PubMed

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER TOKYO  

In 2008 we isolated methicillin-resistant Staphylococcus aureus (MRSA) from an 11-month-old Japanese girl who lived in Saitama, Japan, and suffered from cellulitis of the lower thigh and sepsis. The MRSA (strain NN47) belonged to multilocus sequence type (ST) 8 and exhibited spa363 (t024), agr1, staphylococcal cassette chromosome mec (SCCmec) type IVa, and coagulase type III. It was positive for Panton-Valentine leukocidin (PVL) and the arginine catabolic mobile element (ACME). Pulsed-field gel electrophoresis (PFGE) demonstrated that the MRSA was the USA300 clone, which is the predominant community-acquired MRSA (CA-MRSA) in the US. Strain NN47 was divergent, in terms of the spa type and patterns of PFGE and plasmids, from the USA300-0114 type strain or USA300 strain NN36, previously isolated from a visitor (Indian girl) from the US. Strain NN47 was resistant to erythromycin, in addition to beta-lactam agents (e.g., oxacillin). These data demonstrate the first emergence of the USA300 clone in Japanese children who have never been abroad and have had no contact with foreigners (and therefore, the first USA300 spread in Japan), and also emergence of multiple divergent strains of the USA300 clone in Japan. Because the USA300 clone is highly transmissible and virulent, surveillance of the USA300 clone is needed.

DOI： 10.1007/s10156-010-0051-y

Web of Science

PubMed

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Language：Japanese   Publisher：(一社)日本医学教育学会  

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Language：Japanese   Publisher：日本シミュレーション医療教育学会  

ロシア極東医科大学で開催された"Student Surgical Olympiad"極東地区予選に参加した。大会は、学生の外科領域への興味を促すこと、外科領域へ進むことを希望する学生に早期から実践的外科技術を修得させることを目的としていた。大会は外科手技を中心とした16課題で3日間に渡り行われ、上位2チームにはモスクワで行われる本選への出場権が与えられた。課題達成に必要な医療知識、手技は臨床に即した内容であった。参加学生の熱意は非常に高く、学生外科サークルを通じた事前の十分な準備により、課題への対応が可能となっていた。専門性の高い知識、技能を低学年から修得するためのサポート体制、学生オリンピックの開催は、シミュレーション教育の普及、進歩に寄与し、学生の能動的学習を促す効果がある。(著者抄録)

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Language：Japanese   Publisher：(一社)日本医学教育学会  

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Language：Japanese   Publisher：日本シミュレーション医療教育学会  

ロシア極東医科大学で開催された"Student Surgical Olympiad"極東地区予選に参加した。大会は、学生の外科領域への興味を促すこと、外科領域へ進むことを希望する学生に早期から実践的外科技術を修得させることを目的としていた。大会は外科手技を中心とした16課題で3日間に渡り行われ、上位2チームにはモスクワで行われる本選への出場権が与えられた。課題達成に必要な医療知識、手技は臨床に即した内容であった。参加学生の熱意は非常に高く、学生外科サークルを通じた事前の十分な準備により、課題への対応が可能となっていた。専門性の高い知識、技能を低学年から修得するためのサポート体制、学生オリンピックの開催は、シミュレーション教育の普及、進歩に寄与し、学生の能動的学習を促す効果がある。(著者抄録)

CiNii Article

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Language：Japanese   Publisher：(一社)日本病理学会  

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