2021/10/27 更新

写真a

オカモト ケイイチロウ
岡本 圭一郎
OKAMOTO Keiichiro
所属
教育研究院 医歯学系 歯学系列 准教授
医歯学総合研究科 口腔生命科学専攻 摂食環境制御学 准教授
職名
准教授
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外部リンク

学位

  • 博士(医学) ( 2006年2月   和歌山県立医科大学 )

研究キーワード

  • 脊髄

  • 報酬系

  • 青斑核

  • 酒粕

  • 日本酒

  • 慢性痛

  • 大縫線核

  • photophobia

  • 角膜痛

  • ストレス

  • 下行性疼痛制御系

  • エストロゲン

  • 神経科学

  • 嚥下

  • 痛み

  • 三叉神経脊髄路核尾側亜核

  • 口腔顔面痛

研究分野

  • ライフサイエンス / 外科系歯学  / 痛み

  • ライフサイエンス / 病態神経科学

  • ライフサイエンス / 常態系口腔科学  / 疼痛 神経科学 生理学 嚥下

経歴(researchmap)

  • 新潟大学大学院医歯学総合研究科   口腔生理学分野   准教授

    2015年8月 - 現在

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  • University of Minnesota School of Dentistry   Research Associate

    2006年8月 - 2015年6月

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  • 和歌山県立医科大学   生理学第1講座 (医学博士修得)   助教

    2004年4月 - 2006年7月

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  • 和歌山県立医科大学   解剖学第2講座   研究生

    2004年1月 - 2004年3月

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  • Brown University, Medical School. RI Hospital   Research Associate

    2001年2月 - 2003年12月

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  • 和歌山県立医科大学   解剖学第2講座   研究生

    1999年4月 - 2001年1月

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  • 和歌山県立医科大学   歯科口腔外科   臨床研究医

    1998年4月 - 1999年3月

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  • 橋本市民病院   歯科口腔外科 医員

    1996年10月 - 1998年3月

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  • 和歌山県立医科大学   歯科口腔外科   臨床研究医

    1995年10月 - 1996年8月

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  • 日本赤十字社和歌山医療センター   麻酔科   研修医

    1995年4月 - 1995年9月

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  • 和歌山県立医科大学   歯科口腔外科   診療医(研修医)

    1993年4月 - 1995年3月

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経歴

  • 新潟大学   医歯学総合研究科 口腔生命科学専攻 摂食環境制御学   准教授

    2015年7月 - 現在

学歴

  • 福岡県立九州歯科大学

    1987年4月 - 1993年3月

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  • 清風南海高校

    1984年4月 - 1987年3月

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所属学協会

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委員歴

  • 歯科薬物療法学会   評議員  

    2021年4月 - 現在   

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    団体区分:学協会

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  • 日本口腔顔面痛学会   学術雑誌編集委員会・副委員長  

    2018年4月 - 現在   

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    団体区分:学協会

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  • 日本生理学会   教育委員  

    2017年4月 - 2020年3月   

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    団体区分:学協会

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留学歴

  • ブラウン大学医学部   リサーチアソシエイト

    2000年1月 - 2003年12月

取得資格

  • 歯科医師

 

論文

  • Effects of rice fermented extracts, "Sake Lees", on the functional activity of odontoblast-like cells (KN-3 cells). 査読

    Keiichiro Okamoto, Yoshito Kakihara, Naoto Ohkura, Aiko Tohma, Ayako Washio, Chiaki Kitamura, Yuichiro Noiri, Kensuke Yamamura, Makio Saeki

    Odontology   2021年9月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This study was designed to investigate the effects of Sake Lees extracts (SLE, Sake Kasu) on the functional activity of odontoblastic cells and tooth pulp of the rats. For in vitro studies, a rat clonal odontoblast-like cell line, KN-3 cells were cultured. SLE significantly decreased KN-3 cell proliferation, but showed no significant cytotoxicity. SLE effects on several protein productions of KN-3 cells were compared with PBS. SLE and PBS increased alkaline phosphatase (ALP), dentin sialoprotein (DSP), and osterix in a day-course dependent manner, while SLE increased the induction of ALP on day 9-21 and DSP on day 15-21. SLE also increased Runx2 expression on day 3 and 9 compared to PBS. Alizarin Red stainings revealed that SLE showed a subtle increase in mineralization of KN-3 cells on day 15 and 21. A histological investigation was conducted to assess if SLE induced reparative dentin formation after direct capping at the exposed tooth pulp in rats, suggesting that SLE could increase the reparative dentin formation more than PBS. These findings suggest that Sake Lees could have functional roles in the alterations of odontoblastic activity, which might influence the physiology of the tooth pulp.

    DOI: 10.1007/s10266-021-00654-9

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  • 繰り返しトレッドミル運動は社会的敗北ストレスによる顎顔面領域の侵害応答増大を抑制する

    長谷川 真奈, 岡本 圭一郎, 藤井 規孝, 山村 健介

    Journal of Oral Biosciences Supplement   2020   151 - 151   2020年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • Modulatory effects of repeated psychophysical stress on masseter muscle nociception in the nucleus raphe magnus of rats. 査読

    Shiho Shimizu, Yosuke Nakatani, Masayuki Kurose, Hiroki Imbe, Nobuyuki Ikeda, Ritsuo Takagi, Kensuke Yamamura, Keiichiro Okamoto

    Journal of Oral Science   62 ( 2 )   231 - 235   2020年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Psychophysical stress can cause neural changes that increase nociception in the orofacial region, particularly the masseter muscle (MM). The nucleus raphe magnus (NRM), which is located in the brain stem, serves the crucial role of regulating nociception through descending modulatory pain control. However, it remains unclear if neural activities in the NRM are affected under psychophysical stress conditions. This study conducted experiments to assess (1) whether neural activity, indicated by Fos expression in an NRM that has experienced MM injury, is affected by the stress of repeated forced swim tests (FST); and (2) whether the selective serotonin reuptake inhibitor fluoxetine administered daily after an FST could affect the number of Fos-positive neurons in the NRM. Results revealed that the stress from repeated FSTs significantly increased the number of Fos-positive neurons in an NRM that had been affected by MM injury. Fluoxetine inhibited increases in the number of Fos-positive neurons in the NRM that occurred as a result of FSTs, but this was not observed in sham rats. These findings indicate that the stress from FSTs could increase nociceptive neural activity in an NRM that has experienced MM injury. This could be due, in part, to changes in serotonergic mechanisms.

    DOI: 10.2334/josnusd.19-0320

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  • Daily administration of Sake Lees (Sake Kasu) reduced psychophysical stress-induced hyperalgesia and Fos responses in the lumbar spinal dorsal horn evoked by noxious stimulation to the hindpaw in the rats. 査読

    Shimizu S, Nakatani Y, Kakihara Y, Taiyoji M, Saeki M, Takagi R, Yamamura K, Okamoto K

    Bioscience, Biotechnology, and Biochemistry   84 ( 1 )   159 - 170   2020年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/09168451.2019.1662278

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  • 筋電図波形から官能評価を検出する試み

    黒瀬 雅之, 長谷川 真奈, 岡本 圭一郎, 山村 健介, 佐藤 大祐

    日本咀嚼学会雑誌   29 ( 2 )   114 - 114   2019年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本咀嚼学会  

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  • 反復的な強制水泳ストレスが誘発する吻側廷髄腹側部ニューロンの応答特性の変調

    黒瀬 雅之, 長谷川 真奈, 佐藤 義英, 藤井 規孝, 山村 健介, 岡本 圭一郎

    Journal of Oral Biosciences Supplement   2019   322 - 322   2019年10月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • Dry eye sensitizes cool cells to capsaicin-induced changes in activity via TRPV1. 査読

    Hatta A, Kurose M, Sullivan C, Okamoto K, Fujii N, Yamamura K, Meng ID

    Journal of neurophysiology   121 ( 6 )   2191 - 2201   2019年6月

  • Japanese Rice Wine can reduce psychophysical stress-induced depression-like behaviors and Fos expression in the trigeminal subnucleus caudalis evoked by masseter muscle injury in the rats. 査読

    Nakatani Y, Kakihara Y, Shimizu S, Kurose M, Sato T, Kaneoke M, Saeki M, Takagi R, Yamamura K, Okamoto K

    Bioscience, Biotechnology, and Biochemistry   83   155 - 165   2019年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    添付ファイル: 中谷日本酒論文2019 のコピー.pdf

    DOI: 10.1080/09168451.2018.1524705

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  • ディープラーニングによる「食べ易さ」「飲み込み易さ」判定の可能性

    黒瀬 雅之, 長谷川 真奈, 岡本 圭一郎, 山田 好秋, 山村 健介, 佐藤 大祐

    日本咀嚼学会雑誌   28 ( 2 )   77 - 77   2018年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本咀嚼学会  

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  • Inhibitory effects of fluoxetine, an antidepressant drug, on masseter muscle nociception at the trigeminal subnucleus caudalis and upper cervical spinal cord regions in a rat model of psychophysical stress 査読

    Yosuke Nakatani, Masayuki Kurose, Shiho Shimizu, Mana Hasegawa, Nobuyuki Ikeda, Kensuke Yamamura, Ritsuo Takagi, Keiichiro Okamoto

    Experimental Brain Research   236 ( 8 )   1 - 13   2018年5月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Verlag  

    This study aimed to determine whether psychophysical stress conditionings had facilitatory effects on masseter muscle nociception in the central nervous system via serotonergic mechanisms in rats. Two experiments were conducted to assess: (1) whether repeated forced swim stress for 3 days increased the number of Fos-positive neurons evoked by masseter muscle injury due to formalin injection
    and (2) whether serotonin-reuptake inhibitor, fluoxetine, administered daily after each stress conditioning, had modulatory roles on Fos expression. The number of Fos-positive cells was quantified in several areas within the trigeminal subnucleus caudalis (Vc) and upper cervical spinal cord regions (Vc areas), including the ventrolateral area of the trigeminal subnucleus interpolaris/Vc transition, and the middle or caudal portion of the Vc regions, since nociceptive neural activity in the Vc region could play critical roles in deep craniofacial nociception. We found that forced swim stress conditionings increased depression-like behaviors, which was prevented by fluoxetine. Repeated forced swim stress significantly increased Fos expression in all Vc areas compared with those of non-stressed rats, while systemic administration of fluoxetine significantly decreased Fos expression in all areas, but mainly in the caudal Vc region, in stressed rats. Fluoxetine had no effect on Fos expression in non-stressed rats. These results indicate that repeated forced swim stress conditionings increase Fos expression in the Vc areas, and the contribution of serotonergic mechanisms to masseter muscle nociception could be greater in stressed rats than in sham rats. These results support the hypothesis that changes in brain function, including serotonergic mechanisms, in the Vc areas play critical roles in enhanced masseter muscle nociceptive responses under psychophysical stress conditions.

    DOI: 10.1007/s00221-018-5297-0

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  • Differential Response Pattern of Oropharyngeal Pressure by Bolus and Dry Swallows 査読

    Mana Hasegawa, Masayuki Kurose, Keiichiro Okamoto, Yoshiaki Yamada, Takanori Tsujimura, Makoto Inoue, Taisuke Sato, Takatsune Narumi, Noritaka Fujii, Kensuke Yamamura

    Dysphagia   33 ( 1 )   83 - 90   2018年2月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer New York LLC  

    The aim of this study was to determine if bolus and dry swallow showed similar pressure changes in the oropharynx using our newly developed device. A unique character of it includes that baropressure can be measured with the sensor being placed in the balloon and can assess the swallowing mechanics in terms of pressure changes in the oropharynx with less influences of direct contacts of boluses and oropharyngeal structures during swallow indirectly. Fifteen healthy subjects swallowed saliva (dry), 15 ml of water, 45 ml of water, and 15 ml of two different types of food in terms of viscosity (potage soup-type and mayonnaise-type foods). Suprahyoid muscle activity was recorded simultaneously. Three parameters, area under the curve (AUC), peak amplitude, and duration of pressure, were analyzed from each swallow. Almost all of the bolus swallowing events had biphasic baropressure responses consisting of an early phase and late phase (99%), whereas 90% of the saliva swallowing events had a single phase. AUC, peak, and duration displayed greater effects during the late phase than during the early phase. Baropressure of the early phase, but not of the late phase, significantly increased with increasing volume
    however, small but significant viscosity effects on pressure were seen during both phases. Peak pressure of the late phase was preceded by maximum muscle activity, whereas that of the early phase was seen when muscle activity displayed a peak response. These findings indicated that our device with the ability to measure baropressure has the potential to provide additional parameter to assess the swallow physiology, and biphasic baropressure responses in the early and late phases could reflect functional aspects of the swallowing reflexes.

    DOI: 10.1007/s00455-017-9836-9

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  • Guide to Enhancing Swallowing Initiation: Insights from Findings in Healthy Subjects and Dysphagic Patients. 査読

    Yamamura K, Kurose M, Okamoto K

    Current physical medicine and rehabilitation reports   6 ( 3 )   178 - 185   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s40141-018-0192-y

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  • 心理ストレスが顎顔面痛を増大させる脳神経メカニズム 招待 査読

    清水志保 , 中谷暢佑 , 岡本圭一郎

    日本口腔顔面痛学会雑誌   11 ( 1 )   1 - 8   2018年

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    担当区分:最終著者, 責任著者   記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    添付ファイル: 11_1.pdf

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  • TRIGEMINAL BRAINSTEM MODULATION OF PERSISTENT ORBICULARIS OCULI MUSCLE ACTIVITY IN A RAT MODEL OF DRY EYE 査読

    Mostafeezur Rahman, Kazunari Shiozaki, Keiichiro Okamoto, Randall Thompson, David A. Bereiter

    NEUROSCIENCE   349   208 - 219   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Altered corneal reflex activity is a common feature of dry eye disease (DE). Trigeminal sensory nerves supply the ocular surface and terminate at the trigeminal interpolaris/caudalis (ViVc) transition and spinomedullary (VcC1) regions. Although both regions contribute to corneal reflexes, their role under dry eye conditions is not well defined. This study assessed the influence of local inhibitory and excitatory amino acid neurotransmission at the ViVc transition and VcC1 regions on hypertonic saline (HS) evoked orbicularis oculi muscle activity (OOemg) in urethane-anesthetized male rats after exorbital gland removal (DE). HS increased the magnitude of long-duration OOemg activity (OOemgL, >200 ms) in DE compared to sham rats, while short-duration OOemg activity (OOemgS, <200 ms) was similar for both groups. Inhibition of the ViVc transition by muscimol, a GABA(A) receptor agonist, greatly reduced HS-evoked OOemgL activity in DE rats, whereas injections at the VcC1 region had only minor effects in both groups. Blockade of GABA(A) receptors by bicuculline methiodide at the ViVc transition or VcC1 region increased HS-evoked OOemgL activity in DE rats. Blockade of N-methyl-D-aspartate (NMDA) receptors at either region reduced HS-evoked OOemgL activity in DE and sham rats. GABA alpha beta 3 receptor density was reduced at the ViVc transition, while NMDA receptor density was increased at both regions in DE rats. Loss of GABAergic inhibition at the ViVc transition coupled with enhanced NMDA excitatory amino acid neurotransmission at the ViVc transition and the VcC1 region likely contribute to altered corneal reflexes under dry eye conditions. Published by Elsevier Ltd on behalf of IBRO.

    DOI: 10.1016/j.neuroscience.2017.03.003

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  • Bilateral increases in ERK activation at the spinomedullary junction region by acute masseter muscle injury during temporomandibular joint inflammation in the rats 査読

    Masayuki Kurose, Hiroki Imbe, Yosuke Nakatani, Mana Hasegawa, Noritaka Fujii, Ritsuo Takagi, Kensuke Yamamura, Emiko Senba, Keiichiro Okamoto

    EXPERIMENTAL BRAIN RESEARCH   235 ( 3 )   913 - 921   2017年3月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    We determined the role of persistent monoarthritis of temporomandibular joint region (TMJ) on bilateral masseter muscle (MM) nociception in male rats using orofacial nocifensive behaviors, phosphorylated extracellular signal-regulated kinase and Fos induction at the trigeminal subnucleus caudalis/upper cervical spinal cord (Vc/C-2) region in response to formalin injection to the MM region. TMJ inflammation was induced by local injection of CFA into the left TMJ region. Orofacial nocifensive behaviors evoked by formalin injection ipsilateral or contralateral to the TMJ inflammation appeared to be increased at 1-14 days or at 1, 10 and 14 days after induction of TMJ inflammation, respectively, while increases in behavioral duration were seen mainly in the late phase rather than the early phase. The number of pERK positive cells was investigated in superficial laminae at the Vc/C-2 region at 3, 10, 20, 60 and 80 min after MM stimulation with formalin at 14 days after TMJ inflammation. TMJ-inflamed rats displayed greater responses of pERK expression by the ipsilateral MM stimulation at 3-60 min, while contralateral MM stimulation increased pERK expression at 3, 10 and 20 min compared to non-CFA rats. Fos expression by MM stimulation was increased at 14 days after induction of TMJ inflammation regardless of the affected side. These findings showed that persistent TMJ inflammation for 10 and 14 days is sufficient to enhance MM nociception indicated by behaviors and neural responses in superficial laminae at the Vc/C-2 region.

    DOI: 10.1007/s00221-016-4852-9

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  • Use of a Novel Device to Assess Intraoral and Intrapharyngeal Baropressure during Sound Production 査読

    Toshiki Sakuma, Masayuki Kurose, Keiichiro Okamoto, Mana Hasegawa, Noritaka Fujii, Yousuke Nakatani, Ritsuo Takagi, Taisuke Sato, Yasumitsu Kodama, Rei Ominato, Kensuke Yamamura, Yoshiaki Yamada

    FOLIA PHONIATRICA ET LOGOPAEDICA   68 ( 6 )   274 - 281   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KARGER  

    Objective: We developed a novel device that simultaneously measures oral and intrapharyngeal baropressure. The transducer has the advantage that it can be placed in any region. We determined the effect of different speech samples on baropressure in these regions. Patients and Methods: Seven healthy individuals produced speech samples comprising vowels and consonants (e.g., /aka/, /apa/, and /ash/). Two transducers were installed into the experimental plate at the incisive papillae and center of the Ah-line; a third transducer was placed in the mid-pharyngeal cavity. During each task, 3 parameters were analyzed: peak pressure, mean pressure, and the temporal relationship between sound signals and pressure changes. Results: The mean pressure did not change during the production of a single vowel; however, the pressure transiently increased during the production of the speech samples, depending on the place of articulation. Moreover, the place of articulation affected the onset and peak timing of pressure changes. Conclusions: These findings indicate that pressure changes during the production of speech samples reflect the functional aspects of speech production. In particular, simultaneous pressure recordings at multiple locations would provide precise information about speech production, compared to pressure studies that used a single pressure transducer. (c) 2017 S. Karger AG, Basel

    DOI: 10.1159/000481530

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  • Chemical Sensing Regulates Mastication/Swallowing 査読

    Kensuke Yamamura, Masayuki Kurose, Keiichiro Okamoto

    CURRENT PHARMACEUTICAL DESIGN   22 ( 15 )   2279 - 2284   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BENTHAM SCIENCE PUBL LTD  

    Mastication and swallowing are the first stage of digestion involving several motor processes such as food intake, intra-oral food transport, bolus formation and chewing and swallowing reflex. These complicated motor functions are accomplished by the well-coordinated activities in the jaw, hyoid, tongue, facial and pharyngeal muscles. Although the basic activity patterns of these movements are controlled by the brainstem pattern generators, these movements generate various peripheral sensory inputs. Among the sensory inputs, it is well-known that somatic sensory inputs play important roles in reflexively modulating the movements so that the final motor outputs fit the environmental demand. However, little is known about the effects of chemical sensory inputs such as taste and olfaction originating from the ingested foods by these movements. A possible reason could be raised that cognition of the chemical sensory inputs at the higher brain also influences the movements, so it is difficult to discuss the neural mechanisms underlying the observed effect. In this review, we focus on the effects of chemical sensory inputs on the masticatory movements and initiation of swallowing. We first summarize chemical sensory inputs occurring during mastication and swallowing, and their receptive mechanisms. In addition, we will introduce the effect of application of monosodium L-glutamate (MSG) solution as an umami taste to the oropharynx on the swallow initiation which is involuntary controlled and the possible neural mechanisms underlying this effect is discussed.

    DOI: 10.2174/1381612822666160216151150

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  • INHIBITION OF TEMPOROMANDIBULAR JOINT INPUT TO MEDULLARY DORSAL HORN NEURONS BY 5HT3 RECEPTOR ANTAGONIST IN FEMALE RATS 査読

    K. Okamoto, A. Katagiri, M. Rahman, R. Thompson, D. A. Bereiter

    NEUROSCIENCE   299   35 - 44   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Repeated forced swim (FS) conditioning enhances nociceptive responses to temporomandibular joint (TMJ) stimulation in female rats. The basis for FS-induced TMJ hyperalgesia remains unclear. To test the hypothesis that serotonin 3 receptor (5HT3R) mechanisms contribute to enhanced TMJ nociception after FS, ovariectomized female rats were treated with estradiol and subjected to FS for three days. On day 4, rats were anesthetized with isoflurane and TMJ-responsive neurons were recorded from superficial and deep laminae at the trigeminal subnucleus caudalis/upper cervical (Vc/C1-2) region and electromyographic (EMG) activity was recorded from the masseter muscle. Only Vc/C1-2 neurons activated by intra-TMJ injections of ATP were included for further analysis. Although neurons in both superficial and deep laminae were activated by ATP, only neurons in deep laminae displayed enhanced responses after FS. Local application of the 5HT3R antagonist, ondansetron (OND), at the Vc/C1-2 region reduced the ATP-evoked responses of neurons in superficial and deep laminae and reduced the EMG response in both sham and FS rats. OND also decreased the spontaneous firing rate of neurons in deep laminae and reduced the high-threshold convergent cutaneous receptive field area of neurons in superficial and deep laminae in both sham and FS rats. These results revealed that central application of a 5HT3R antagonist, had widespread effects on the properties of TMJ-responsive neurons at the Vc/C1-2 region and on jaw muscle reflexes under sham and FS conditions. It is concluded that 5HT3R does not play a unique role in mediating stress-induced hyperalgesia related to TMJ nociception. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Sensitization of trigeminal brainstem pathways in a model for tear deficient dry eye 査読

    Mostafeezur Rahman, Keiichiro Okamoto, Randall Thompson, Ayano Katagiri, David A. Bereiter

    PAIN   156 ( 5 )   942 - 950   2015年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Chronic dry eye disease (DE) is associated with an unstable tear film and symptoms of ocular discomfort. The characteristics of symptoms suggest a key role for central neural processing; however, little is known about central neuroplasticity and DE. We used a model for tear deficient DE and assessed effects on eye blink behavior, orbicularis oculi muscle activity (OOemg), and trigeminal brainstem neural activity in male rats. Ocular-responsive neurons were recorded at the interpolaris/caudalis transition (Vi/Vc) and Vc/upper cervical cord (Vc/C1) regions under isoflurane, whereas OOemg activity was recorded under urethane. Spontaneous tear volume was reduced by similar to 50% at 14 days after exorbital gland removal. Hypertonic saline-evoked eye blink behavior in awake rats was enhanced throughout the 14 days after surgery. Saline-evoked neural activity at the Vi/Vc transition and in superficial and deep laminae at the Vc/C1 region was greatly enhanced in DE rats. Neurons from DE rats classified as wide dynamic range displayed enlarged convergent periorbital receptive fields consistent with central sensitization. Saline-evoked OOemg activity was markedly enhanced in DE rats compared with controls. Synaptic blockade at the Vi/Vc transition or the Vc/C1 region greatly reduced hypertonic saline-evoked OOemg activity in DE and sham rats. These results indicated that persistent tear deficiency caused sensitization of ocular-responsive neurons at multiple regions of the caudal trigeminal brainstem and enhanced OOemg activity. Central sensitization of ocular-related brainstem circuits is a significant factor in DE and likely contributes to the apparent weak correlation between peripheral signs of tear dysfunction and symptoms of irritation.

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  • EVIDENCE FOR TRPA1 INVOLVEMENT IN CENTRAL NEURAL MECHANISMS IN A RAT MODEL OF DRY EYE 査読

    A. Katagiri, R. Thompson M. Rahman, K. Okamoto, A. Bereiter

    NEUROSCIENCE   290   204 - 213   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Dry eye (DE) disease is commonly associated with ocular surface inflammation, an unstable tear film and symptoms of irritation. However, little is known about the role of central neural mechanisms in DE. This study used a model for persistent aqueous tear deficiency, exorbital gland removal, to assess the effects of mustard oil (MO), a transient receptor potential ankyrin (TRPA1) agonist, on eyeblink and eyewipe behavior and Fos-like immunoreactivity (Fos-LI) in the trigeminal brainstem of male rats. Spontaneous tear secretion was reduced by about 50% and spontaneous eyeblinks were increased more than 100% in DE rats compared to sham rats. MO (0.02-0.2%) caused dose-related increases in eyeblink and forelimb eyewipe behavior in DE and sham rats. Exorbital gland removal alone was sufficient to increase Fos-LI at the ventrolateral pole of trigeminal interpolaris/caudalis (Vi/Vc) transition region, but not at more caudal regions of the trigeminal brainstem. Under barbiturate anesthesia ocular surface application of MO (2-20%) produced Fos-LI in the Vi/Vc transition, in the mid-portions of Vc and in the trigeminal caudalis/upper cervical spinal cord (Vc/C1) region that was significantly greater in DE rats than in sham controls. MO caused an increase in Fos-LI ipsilaterally in superficial laminae at the mid-Vc and Vc/C1 regions in a dose-dependent manner. Smaller, but significant, increases in Fos-LI also were seen in the contralateral Vc/C1 region in DE rats. TRPA1 protein levels in trigeminal ganglia from DE rats ipsilateral and contralateral to gland removal were similar. Persistent tear reduction enhanced the behavioral and trigeminal brainstem neural responses to ocular surface stimulation by MO. These results suggested that TRPA1 mechanisms play a significant role in the sensitization of ocular-responsive trigeminal brainstem neurons in this model for tear deficient DE. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Activation of rostral ventromedial medulla neurons by noxious stimulation of cutaneous and deep craniofacial tissues 査読

    Sergey G. Khasabov, Patrick Malecha, Joseph Noack, Janneta Tabakov, Keiichiro Okamoto, David A. Bereiter, Donald A. Simone

    JOURNAL OF NEUROPHYSIOLOGY   113 ( 1 )   14 - 22   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    The rostral ventromedial medulla (RVM) projects to the medullary and spinal dorsal horns and is a major source of descending modulation of nociceptive transmission. Traditionally, neurons in the RVM are classified functionally as ON, OFF, and NEUTRAL cells on the basis of responses to noxious cutaneous stimulation of the tail or hind paw. ON cells facilitate nociceptive transmission, OFF cells are inhibitory, whereas NEUTRAL cells are unresponsive to noxious stimuli and their role in pain modulation is unclear. Classification of RVM neurons with respect to stimulation of craniofacial tissues is not well defined. In isoflurane-anesthetized male rats, RVM neurons first were classified as ON (25.5%), OFF (25.5%), or NEUTRAL (49%) cells by noxious pinch applied to the hind paw. Pinching the skin overlying the temporomandibular joint (TMJ) altered the proportions of ON (39.2%), OFF (42.2%), and NEUTRAL (19.6%) cells. To assess the response of RVM cells to specialized craniofacial inputs, adenosine triphosphate (ATP; 0.01-1 mM) was injected into the TMJ and capsaicin (0.1%) was applied to the ocular surface. TMJ and ocular surface stimulation also resulted in a reduced proportion of NEUTRAL cells compared with hind paw pinch. Dose-effect analyses revealed that ON and OFF cells encoded the intra-TMJ concentration of ATP. These results suggest that somatotopy plays a significant role in the functional classification of RVM cells and support the notion that NEUTRAL cells likely are subgroups of ON and OFF cells. It is suggested that a portion of RVM neurons serve different functions in modulating craniofacial and spinal pain conditions.

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  • TRIGEMINAL PATHWAYS FOR HYPERTONIC SALINE- AND LIGHT-EVOKED CORNEAL REFLEXES 査読

    M. Rahman, K. Okamoto, R. Thompson, D. A. Bereiter

    NEUROSCIENCE   277   716 - 723   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Cornea-evoked eyeblinks maintain tear film integrity on the ocular surface in response to dryness and protect the eye from real or potential damage. Eyelid movement following electrical stimulation has been well studied in humans and animals; however, the central neural pathways that mediate protective eyeblinks following natural nociceptive signals are less certain. The aim of this study was to assess the role of the trigeminal subnucleus interpolaris/caudalis (Vi/Vc) transition and subnucleus caudalis/upper cervical cord (Vc/C1) junction regions on orbicularis oculi electromyographic (OOemg) activity evoked by ocular surface application of hypertonic saline or exposure to bright light in urethane anesthetized male rats. The Vi/Vc and Vc/C1 regions are the main sites of termination for trigeminal afferent nerves that supply the ocular surface, while hypertonic saline (saline = 0.15-5 M) and bright light (light = 5k-20k lux) selectively activate ocular surface and intraocular trigeminal nerves, respectively, and excite second-order neurons at the Vi/Vc and Vc/C1 regions. Integrated OOemg activity, ipsilateral to the applied stimulus, increased with greater stimulus intensities for both modalities. Lidocaine applied to the ocular surface inhibited OOemg responses to hypertonic saline, but did not alter the response to light. Lidocaine injected into the trigeminal ganglion blocked completely the OOemg responses to hypertonic saline and light indicating a trigeminal afferent origin. Synaptic blockade by cobalt chloride of the Vi/Vc or Vc/C1 region greatly reduced OOemg responses to hypertonic saline and bright light. These data indicate that OOemg activity evoked by natural stimuli known to cause irritation or discomfort in humans depends on a relay in both the Vi/Vc transition and Vc/C1 junction regions. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Posterior hypothalamic modulation of ocular-responsive trigeminal subnucleus caudalis neurons is mediated by Orexin-A and Orexin1 receptors 査読

    Ayano Katagiri, Keiichiro Okamoto, Randall Thompson, Mostafeezur Rahman, David A. Bereiter

    EUROPEAN JOURNAL OF NEUROSCIENCE   40 ( 4 )   2619 - 2627   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Orexin-A (OxA) is synthesized in posterior and lateral regions of the hypothalamus and contributes to homeostatic regulation of body functions including pain modulation. To determine if orexinergic mechanisms contribute to posterior hypothalamus (PH)-induced modulation of ocular input to subnucleus caudalis/upper cervical (Vc/C1) neurons, the orexin-1 receptor antagonist SB334867 was applied to the dorsal brainstem surface prior to PH disinhibition, by bicuculline methiodide, in male rats under iso-flurane anesthesia. Ocular input to Vc/C1 units by bright light or hypertonic saline was markedly reduced by PH disinhibition and reversed completely by local Vc/C1 application of SB334867. OxA applied to the Vc/C1 surface mimicked the effects of PH disinhibition in a dose-dependent manner. OxA-induced inhibition was prevented by co-application of SB334867, but not by the orexin-2 receptor antagonist TCS Ox2 29. PH disinhibition and local OxA application also reduced the high threshold convergent cutaneous receptive field area of ocular units, suggesting widespread effects on somatic input to Vc/C1 ocular units. Vc/C1 application of OxA or SB334867 alone did not affect the background discharge of ocular units and suggested that the PH-OxA influence on ocular unit activity was not tonically active. Vc/C1 application of OxA or SB334867 alone also did not alter mean arterial pressure, whereas PH disinhibition evoked prompt and sustained increases. These results suggest that stimulus-evoked increases in PH outflow acts through OxA and orexin-1 receptors to alter the encoding properties of trigeminal brainstem neurons responsive to input from the ocular surface and deep tissues of the eye.

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  • POSTERIOR HYPOTHALAMIC MODULATION OF LIGHT-EVOKED TRIGEMINAL NEURAL ACTIVITY AND LACRIMATION 査読

    A. Katagiri, K. Okamoto, R. Thompson, D. A. Bereiter

    NEUROSCIENCE   246   133 - 141   2013年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Enhanced light sensitivity is a common feature of many neuro-ophthalmic conditions and some chronic headaches. Previously we reported that the bright light-evoked increases in trigeminal brainstem neural activity and lacrimation depended on a neurovascular link within the eye (Okamoto et al., 2012). However, the supraspinal pathways necessary for these light-evoked responses are not well defined. To assess the contribution of the posterior hypothalamic area (PH), a brain region closely associated with control of autonomic outflow, we injected bicuculline methiodide (BMI), a GABAa receptor antagonist, into the PH and determined its effect on the encoding properties of ocular neurons at the ventrolateral trigeminal interpolaris/caudalis transition (Vi/Vc) and caudalis/upper cervical cord junction (Vc/C1) regions and on reflex lacrimation in male rats under isoflurane anesthesia. BMI markedly reduced light-evoked (>80%) responses of Vi/Vc and Vc/C1 neurons at 10 min with partial recovery by 50 min after injection. BMI also reduced (>35%) the convergent cutaneous receptive field area of Vi/Vc and Vc/C1 ocular neurons indicating that both intra-ocular and periorbital cutaneous inputs were affected by changes in PH outflow. Light-evoked lacrimation was reduced by >35% at 10 min after BMI, while resting mean arterial pressure increased promptly and remained elevated (>20 mmHg) throughout the 50-min post-injection period. These, results suggested that PH stimulation, acting in part through increased sympathetic activity, significantly inhibited light- and facial skin-evoked activity of ocular neurons at the Vi/Vc and Vc/C1 region. These data provide further support for the hypothesis that autonomic outflow plays a critical role in mediating light-evoked trigeminal brainstem neural activity and reflex lacrimation. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Estrogen status and psychophysical stress modify temporomandibular joint input to medullary dorsal horn neurons in a lamina-specific manner in female rats 査読

    Keiichiro Okamoto, Randall Thompson, Ayano Katagiri, David A. Bereiter

    PAIN   154 ( 7 )   1057 - 1064   2013年7月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Estrogen status and psychological stress contribute to the expression of several chronic pain conditions including temporomandibular muscle and joint disorders (TMJD). Sensory neurons that supply the temporomandibular joint (TMJ) region terminate in laminae I and V of the spinal trigeminal nucleus (Vc/C1-2 region); however, little is known about lamina-specificity and environmental influences on the encoding properties of TMJ brainstem neurons. To test the hypothesis that Vc/C1-2 neurons integrate both interoceptive and exteroceptive signals relevant for TMJ nociception, we recorded TMJ-evoked activity in superficial and deep laminae of ovariectomized rats under high and low estradiol (E2) and stress conditions. Rats received daily injections of low (LE) or high (HE) dose E2 and were subjected to forced swim (FS) or sham swim conditioning for 3 days. The results revealed marked lamina-specificity in that HE rats displayed enhanced TMJ-evoked activity in superficial, but not deep, laminae independent of stress conditioning. By contrast, FS conditioned rats displayed increased background firing and TMJ-evoked activity of neurons in deep, but not superficial, laminae independent of E2 status. FS also enhanced TMJ-evoked masseter muscle activity and suggested the importance of deep dorsal horn neurons in mediating evoked jaw muscle activity. In conclusion, E2 status and psychophysical stress play a significant role in modifying the encoding properties of TMJ-responsive medullary dorsal horn neurons with a marked lamina-specificity. (C) 2013 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.pain.2013.03.009

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  • Trigeminal interpolaris/caudalis transition neurons mediate reflex lacrimation evoked by bright light in the rat 査読

    Keiichiro Okamoto, Akimasa Tashiro, Randall Thompson, Yasuhiro Nishida, David A. Bereiter

    EUROPEAN JOURNAL OF NEUROSCIENCE   36 ( 11 )   3492 - 3499   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Abnormal sensitivity to bright light can cause discomfort or pain and evoke protective reflexes such as lacrimation. Although the trigeminal nerve is probably involved, the mechanism linking luminance to somatic sensory nerve activity remains uncertain. This study determined the effect of bright light on second-order ocular neurons at the ventral trigeminal interpolaris/caudalis transition (Vi/Vc) region, a major termination zone for trigeminal sensory fibers that innervate the eye. Most Vi/Vc neurons (80.9%) identified by responses to mechanical stimulation of the ocular surface also encoded bright light intensity. Light-evoked neural activity displayed a long latency to activation (> 10 s) and required transmission through the trigeminal root ganglion. Light-evoked neural activity was inhibited by intravitreal injection of phenylephrine or l-NG-nitro-arginine methyl ester (L-NAME), suggesting a mechanism coupled to vascular events within the eye. Laser Doppler flowmetry revealed rapid light-evoked increases in ocular blood flow that occurred prior to the increase in Vi/Vc neural activity. Synaptic blockade of the Vi/Vc region by cobalt chloride prevented light-evoked increases in tear volume, whereas blockade at the more caudal spinomedullary junction (Vc/C1) had no effect. In summary, Vi/Vc neurons encoded bright light intensity and were inhibited by drugs that alter blood flow to the eye. These results support the hypothesis that light-responsive neurons at the Vi/Vc transition region are critical for ocular-specific functions such as reflex lacrimation, whereas neurons at the caudal Vc/C1 junction region probably serve other aspects of ocular nociception.

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  • Temporomandibular joint-evoked responses by spinomedullary neurons and masseter muscle are enhanced after repeated psychophysical stress 査読

    Keiichiro Okamoto, Akimasa Tashiro, Zheng Chang, Randall Thompson, David A. Bereiter

    EUROPEAN JOURNAL OF NEUROSCIENCE   36 ( 1 )   2025 - 2034   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Psychological stress is a risk factor for the development of musculoskeletal pain of the head and neck; however, the basis for this relationship remains uncertain. This study tested the hypothesis that psychophysical stress alone was sufficient to alter the encoding properties of spinomedullary dorsal horn neurons and masseter muscle activity in male rats. Repeated forced swim conditioning increased markedly both the background firing rate and temporomandibular joint (TMJ)-evoked activity of neurons in deep dorsal horn, while neurons in superficial laminae were less affected. Stress also increased the responses to stimulation of facial skin overlying the TMJ of neurons in deep and superficial dorsal horn. TMJ-evoked masseter muscle activity was enhanced significantly in stressed rats, an effect that was reduced by prior blockade of the spinomedullary junction region. These data indicated that repeated psychophysical stress induced widespread effects on the properties of medullary dorsal horn neurons and masseter muscle activity. The effects of stress were seen preferentially on neurons in deep dorsal horn and included enhanced responses to chemosensory input from the TMJ and mechanical input from overlying facial skin. The stress-induced elevation in TMJ-evoked masseter muscle activity matched well with the changes seen in dorsal horn neurons. It is concluded that the spinomedullary junction region plays a critical role in the integration of psychophysical stress and sensory information relevant for nociception involving deep craniofacial tissues.

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  • DIFFERENTIAL ASCENDING PROJECTIONS OF TEMPOROMANDIBULAR JOINT-RESPONSIVE BRAINSTEM NEURONS TO PERIAQUEDUCTAL GRAY AND POSTERIOR THALAMUS OF MALE AND FEMALE RATS 査読

    Z. Chang, K. Okamoto, D. A. Bereiter

    NEUROSCIENCE   203   230 - 243   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Several craniofacial pain conditions, including temporomandibular joint disorders (TMJDs), are more prevalent in women than men. The basis for sex differences in deep craniofacial pain is not known. The present study compared the magnitude of ascending projections from temporomandibular joint (TMJ)-responsive neurons in trigeminal brainstem with the ventrolateral periaqueductal gray (vIPAG) or posterior nucleus of the thalamus (Po) in males and female rats. Fluorogold (FG) was injected into vIPAG or Po, and TMJ-responsive neurons were identified by Fos-like immunoreactivity (Fos-LI) after mustard oil injection. TMJ-evoked Fos-LI was similar in males and females; however, significant differences in cell counts were seen for FG single-labeled and Fos/FG double-labeled neurons in trigeminal brainstem. After vIPAG injections, the number of FG-labeled neurons in trigeminal subnucleus interpolaris (Vi), ventral interpolaris/caudalis transition (vI-ViNc), and dorsal paratrigeminal region (dPa5) was greater in females than males. The percentage of Fos/FG double-labeled neurons in vl-ViNc and dPa5 after vIPAG injection also was greater in females than males. In contrast, after Po injections, males displayed a greater number of FG-labeled neurons in superficial laminae (Lam I/II) of trigeminal subnucleus caudalis (Vc) and upper cervical spinal cord (C1-2) and deeper laminae (Lam IIIN) at C1-2 than females. The percentage of Fos/FG double-labeled neurons in Lam I/II of Vc after Po injection also was greater in males than females. These data revealed significant sex differences in ascending projections from TMJ-responsive neurons in trigeminal brainstem. Such differences may influence the ability of males and females to recruit autonomic reflexes and endogenous pain control circuits relevant for TMJ nociception. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.neuroscience.2011.11.042

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  • Rapid Estrogenic Effects on TMJ-responsive Brainstem Neurons 査読

    A. Tashiro, K. Okamoto, D. A. Bereiter

    JOURNAL OF DENTAL RESEARCH   91 ( 2 )   210 - 214   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Estrogen status is a risk factor for temporomandibular muscle and joint disorders (TMJD) and other craniofacial pain conditions. The basis for estrogen modulation of pain is poorly understood and has often been attributed to long-term genomic effects. However, estrogens also act rapidly through membrane-initiated mechanisms to alter neural activity. To assess if estrogens act rapidly to affect TMJ-responsive neurons, we applied 17 beta-estradiol (E2) directly at the spinomedullary (Vc/C(1-2)) region, the initial brainstem site for synaptic integration of TMJ sensory signals, while recording single neuron activity. In ovariectomized female rats, E2 rapidly (within 10 minutes) and reversibly reduced TMJ-evoked neural activity at the Vc/C(1-2) region. The effect was estrogen receptor (ER) subtype-specific, since ER beta agonists inhibited, while an ER beta agonist enhanced, evoked activity. A membrane-mediated mechanism was indicated, since the membrane-impermeable analogue, E(2)-BSA, mimicked the inhibitory effect of E2 and was prevented by an ER antagonist. This study demonstrated that E2 acted rapidly, through membrane-mediated pathways, and locally at the Vc/C(1-2) region, to modulate sensory signals from the TMJ region. These results were consistent with the hypothesis that estrogens can act rapidly at the level of the trigeminal brainstem complex to influence sensory integration of TMJ-related information.

    DOI: 10.1177/0022034511428156

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  • NEUROBIOLOGY OF ESTROGEN STATUS IN DEEP CRANIOFACIAL PAIN 査読

    David A. Bereiter, Keiichiro Okamoto

    TRANSLATING MECHANISMS OF OROFACIAL NEUROLOGICAL DISORDER   97   251 - 284   2011年

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:ELSEVIER ACADEMIC PRESS INC  

    DOI: 10.1016/B978-0-12-385198-7.00010-2

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  • Psychophysical stress increases the expression of phospho-CREB, Fos protein and neurokinin-1 receptors in superficial laminae of trigeminal subnucleus caudalis in female rats 査読

    Sara L. Duenes, Randy Thompson, Zheng Chang, Keiichiro Okamoto, David A. Bereiter

    NEUROSCIENCE LETTERS   486 ( 3 )   207 - 210   2010年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER IRELAND LTD  

    Psychological stress and estrogen status are risk factors to develop painful temporomandibular joint disorders (TMJD) however the neural basis for this relationship is not known This study tested the hypothesis that repeated forced swim stress and estradiol treatment alter the phosphorylation of cAMP responsive element-binding protein (pCREB) in trigeminal subnucleus caudal's (Vc) the initial site of sensory input from the TMJ Ovariectomized female rats were given low or high dose estradiol and subjected to repeated forced swim stress for 3 days and on day 4 an intra-TMJ injection of mustard oil or vehicle was given Forced swim alone Increased the number of pCREB-positive neurons independent of estradiol treatment or TMJ stimulation in superficial and deep laminae of Vc Forced swim also increased the number of Fos-positive neurons in superficial laminae and neurokinin-1 receptor mRNA in whole dorsal Vc Independent of estradiol treatment These results indicated that persistent psychophysical stress alone was sufficient to Increase the expression of pCREB and downstream regulated genes associated with enhanced excitability in the caudal medullary dorsal horn a brainstem region thought to be critical for TMJD pain (C) 2010 Elsevier Ireland Ltd All rights reserved

    DOI: 10.1016/j.neulet.2010.09.054

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  • ULTRAVIOLET IRRADIATION OF THE EYE AND Fos-POSITIVE NEURONS INDUCED IN TRIGEMINAL BRAINSTEM AFTER INTRAVITREAL OR OCULAR SURFACE TRANSIENT RECEPTOR POTENTIAL VANILLOID 1 ACTIVATION 査読

    Z. Chang, K. Okamoto, A. Tashiro, D. A. Bereiter

    NEUROSCIENCE   170 ( 2 )   678 - 685   2010年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The interior structures of the eye are well supplied by the trigeminal nerve; however, the function of these afferent fibers is not well defined. The aim of this study was to use c-fos like immunohistochemistry (Fos-LI) to map the trigeminal brainstem complex after intravitreal microinjection or ocular surface application of capsaicin, a selective transient receptor potential vanilloid 1 (TRPV1) agonist in male rats under barbiturate anesthesia. The effect of ocular inflammation on Fos-LI was tested 2 or 7 days after UV irradiation of the eye. In non-inflamed controls, intravitreal capsaicin produced peaks of Fos-LI at the trigeminal subnucleus interpolaris/caudalis (Vi/Vcvl) transition and in superficial laminae at the caudalis/upper cervical cord (Vc/C1) junction regions. At the Vc/C1 junction intravitreal capsaicin induced Fos-LI in a dose-dependent manner, while at the Vi/Vcvl transition responses were similar after vehicle or capsaicin injections. Two days, but not 7 days, after UV irradiation intravitreal and ocular surface capsaicin-evoked Fos-LI at the Vc/C1 junction and nucleus tractus solitarius (NTS) were markedly enhanced, whereas the responses at the Vi/Vcvl transition were not different from non-inflamed controls. More than 80% of trigeminal ganglion neurons labeled after intravitreal microinjection of Fluorogold also expressed immunoreactivity for the TRPV1 receptor. These findings suggested that most intraocular trigeminal sensory nerves serve as nociceptors. The similar pattern and magnitude of Fos-LI after capsaicin suggested that TRPV1-responsive trigeminal nerves that supply intraocular and ocular surface tissues form a unified integrative circuit in the caudal brainstem. Intensity coding of capsaicin concentration and facilitation of Fos-LI expression after UV irradiation strongly supported the hypothesis that the Vc/C1 junction was critical for nociceptive processing related to ocular pain, whereas the Vi/Vcvl transition region likely served other functions in ocular homeostasis under naive and inflamed conditions. Published by Elsevier Ltd on behalf of IBRO.

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  • BEHAVIORAL AND NEUROPHYSIOLOGICAL CORRELATES OF NOCICEPTION IN AN ANIMAL MODEL OF PHOTOKERATITIS 査読

    A. Tashiro, K. Okamoto, Z. Chang, D. A. Bereiter

    NEUROSCIENCE   169 ( 1 )   455 - 462   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Ocular exposure to ultraviolet irradiation (UVR) induces photokeratitis, a common environmental concern that inflames ocular tissues and causes pain. The central neural mechanisms that contribute to the sensory aspects of photokeratitis after UVR are not known. In awake male rats, ocular surface application of hypertonic saline evoked eye wipe behavior that was enhanced 2-3 days after UVR and returned to control levels by 7 days. Similarly, under isoflurane anesthesia, hypertonic saline-evoked activity of ocular neurons in superficial laminae at the trigeminal subnucleus caudalis/cervical (Vc/C1) region was enhanced 2 days, but not 7 days, after UVR. By contrast, the response of neurons at the interpolaris/caudalis (Vi/Vc) transition region to hypertonic saline was not affected by UVR. The background activity and convergent cutaneous receptive field areas of Vc/C1 or Vi/Vc neurons were not affected by UVR. Aqueous humor protein levels were elevated 2 and 7 days after UVR. UVR enhanced nociceptive behavior, after a latent period, with a time course similar to that of ocular neurons in superficial laminae at the Vc/C1 region. The Vc/C1 region plays a key role in primary hyperalgesia induced by UVR, whereas the Vi/Vc region likely mediates other aspects of ocular function. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Bright light activates a trigeminal nociceptive pathway 査読

    Keiichiro Okamoto, Akimasa Tashiro, Zheng Chang, David A. Bereiter

    PAIN   149 ( 2 )   235 - 242   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Bright light can cause ocular discomfort and/or pain; however, the mechanism linking luminance to trigeminal nerve activity is not known. In this study we identify a novel reflex circuit necessary for bright light to excite nociceptive neurons in superficial laminae of trigeminal subnucleus caudalis (Vc/C1). Vc/C1 neurons encoded light intensity and displayed a long delay (>10 s) for activation. Microinjection of lidocaine into the eye or trigeminal root ganglion (TRG) inhibited light responses completely, whereas topical application onto the ocular surface had no effect. These findings indicated that light-evoked Vc/C1 activity was mediated by an intraocular mechanism and transmission through the TRG. Disrupting local vasomotor activity by intraocular microinjection of the vasoconstrictive agents, norepinephrine or phenylephrine, blocked light-evoked neural activity, whereas ocular surface or intra-TRG microinjection of norepinephrine had no effect. Pupillary muscle activity did not contribute since light-evoked responses were not altered by atropine. Microinjection of lidocaine into the superior salivatory nucleus diminished light-evoked Vc/C1 activity and lacrimation suggesting that increased parasympathetic outflow was critical for light-evoked responses. The reflex circuit also required input through accessory visual pathways since both Vc/C1 activity and lacrimation were prevented by local blockade of the olivary pretectal nucleus. These findings support the hypothesis that bright light activates trigeminal nerve activity through an intraocular mechanism driven by a luminance-responsive circuit and increased parasympathetic outflow to the eye. (C) 2010 International Association for the Study of Pain. Published by Elsevier B. V. All rights reserved.

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  • Involvement of descending facilitation from the rostral ventromedial medulla in the enhancement of formalin-evoked nocifensive behavior following repeated forced swim stress 査読

    Hiroki Imbe, Keiichiro Okamoto, Tomohiro Donishi, Emiko Senba, Akihisa Kimura

    BRAIN RESEARCH   1329   103 - 112   2010年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In the present study we examined whether the descending facilitation from the rostral ventromedial medulla (RVM) is required for the enhancement of formalin-evoked nocifensive behavior following repeated forced swim stress. Rats were subjected to forced or sham swim stress for 3 days. Withdrawal latency to noxious thermal stimuli and mechanical withdrawal threshold to von Frey filaments did not change significantly in both groups at 24h after the last stress session. The forced swim stress showed significantly enhanced nocifensive behavior to the subcutaneous administration of formalin at 2 days after the last stress session (1330.1 +/- 62.8 s), compared to the sham swim (1076 +/- 102.4 s, p<0.05) and naive groups (825.9 +/- 83.2 s, p<0.01). The destruction of the RVM with ibotenic acid led to prevent the enhancement of formalin-evoked nocifensive behavior in the forced swim group. These findings suggest that the descending facilitation from the RVM may be involved in the enhancement of formalin-evoked nocifensive behavior following the forced swim stress. (C) 2010 Elsevier B.V. All rights reserved.

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  • NMDA RECEPTOR BLOCKADE REDUCES TEMPOROMANDIBULAR JOINT-EVOKED ACTIVITY OF TRIGEMINAL SUBNUCLEUS CAUDALIS NEURONS IN AN ESTROGEN-DEPENDENT MANNER 査読

    A. Tashiro, K. Okamoto, D. A. Bereiter

    NEUROSCIENCE   164 ( 4 )   1805 - 1812   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Estrogen status is a risk factor in painful temporomandibular disorders (TMJD). Previously we reported that estradiol (E2) enhanced nociceptive processing of TMJ input by neurons in superficial laminae at the spinomedullary (Vc/C(1-2)) region; however, the mechanisms for this enhancement are not known. The present study determined if ionotropic glutamate receptors contribute to TMJ nociceptive processing in an E2-dependent manner. Ovariectomized (OvX) female rats were treated with high E2 (HE2) or low dose E2 (LE2) for 2 days and neural activity was recorded in laminae I-II at the VC/C(1-2) region. TMJ-responsive units were activated by ATP injections into the joint space. ATP-evoked unit responses in HE2 rats were reduced significantly by topical application of the N-methyl-D-aspartate receptor antagonist, D(-)-2-amino-5-phosphonopentanoic acid (AP5) in a dose-related manner, while units from LE2 were not affected. Application of the non-NMDA receptor antagonist, 6,7-dinitroquinoxaline-2,3-dione (DNOX), inhibited the ATP-evoked responses in both groups. Spontaneous activity of TMJ units was not influenced by AP5, whereas it was reduced by DNQX similarly in both groups. The high threshold convergent cutaneous receptive field area of TMJ units was not changed by AP5, whereas DNQX caused a significant reduction in both groups. These results suggest that NMDA-dependent mechanisms contribute to the enhanced ATP-evoked responses of TMJ units in superficial laminae at the Vc/C(1-2) region under high E2 conditions, while non-NMDA-dependent mechanisms modify the encoding properties of TMJ units independent of E2 status. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • CHRONIC INFLAMMATION AND ESTRADIOL INTERACT THROUGH MAPK ACTIVATION TO AFFECT TMJ NOCICEPTIVE PROCESSING BY TRIGEMINAL CAUDALIS NEURONS 査読

    A. Tashiro, K. Okamoto, D. A. Bereiter

    NEUROSCIENCE   164 ( 4 )   1813 - 1820   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway plays a key role in mediating estrogen actions in the brain and neuronal sensitization during inflammation. Estrogen status is a risk factor in chronic temporomandibular muscle/joint (TMJ) disorders; however, the basis for this relationship is not known. The present study tested the hypothesis that estrogen status acts through the MAPK/ERK signaling pathway to alter TMJ nociceptive processing. Single TMJ-responsive neurons were recorded in laminae I-II at the spinomedullary (Vc/C(1-2)) junction in naive ovariectomized (OvX) female rats treated for 2 days with high-dose (20 mu g/day; HE2) or low-dose estradiol (2 mu g/day; LE2) and after chronic inflammation of the TMJ region by complete Freund's adjuvant for 12-14 days. Intra-TMJ injection of ATP (1 mM) was used to activate Vc/C(1-2) neurons. The MAPK/ERK inhibitor (PD98059, 0.01-1 mM) was applied topically to the dorsal VC/C(1-2) surface at the site of recording 10 min prior to each ATP stimulus. In naive HE2 rats, low-dose PD98059 caused a maximal inhibition of ATP-evoked activity, whereas even high doses had only minor effects on units in LE2 rats. By contrast, after chronic TMJ inflammation, PD98059 produced a marked and similar dose-related inhibition of ATP-evoked activity in HE2 and LE2 rats. These results suggested that E2 status and chronic inflammation acted, at least in part, through a common MAPK/ERK-dependent signaling pathway to enhance TMJ nociceptive processing by laminae I-II neurons at the spinomedullary junction region. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • BRIGHT LIGHT PRODUCES Fos-POSITIVE NEURONS IN CAUDAL TRIGEMINAL BRAINSTEM 査読

    K. Okamoto, R. Thompson, A. Tashiro, Z. Chang, D. A. Bereiter

    NEUROSCIENCE   160 ( 4 )   858 - 864   2009年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Excessive discomfort after exposure to bright light often occurs after ocular injury and during headache. Although the trigeminal nerve is necessary for light-evoked discomfort, the mechanisms underlying this phenomenon, often referred to generally as photophobia, are not well defined. Quantitative Fos-like immunoreactivity (Fos-LI) was used to determine the pattern of neuronal activation in the caudal brainstem after bright light stimulation and, secondly, whether a neurovascular mechanism within the eye contributes to this response. Under barbiturate anesthesia, male rats were exposed to low (1 x 10(4) Ix) or high intensity (2 x 104 Ix) light delivered from a thermal neutral source for 30 min (30 s ON, 30 s OFF) and allowed to survive for 90 min. Intensity-dependent increases in Fos-LI were seen in laminae I-II at the trigeminal caudalis/cervical cord junction region (Vc/C1) and nucleus tractus solitarius (NTS). Fos-LI also increased at the trigeminal interpolaris/caudalis transition (Vi/Vc(vI)) and dorsal paratrigeminal (dPa5) regions independent of intensity. Intravitreal injection of norepinephrine greatly reduced light-evoked Fos-LI at the Vc/C1, dPa5 and NTS, but not at the Vi/Vc transition. Lidocaine applied to the ocular surface had no effect on Fos-LI produced in trigeminal brainstem regions. These results suggested that multiple regions of the caudal trigeminal brainstem complex integrate light-related sensory information. Fos-LI produced at the dPa5 and NTS, coupled with norepinephrine-induced inhibition, was consistent with the hypothesis that light-evoked activation of trigeminal brainstem neurons involves an intraocular neurovascular mechanism with little contribution from neurons that supply the ocular surface. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • OCULAR SURFACE-EVOKED Fos-LIKE IMMUNOREACTIVITY IS ENHANCED IN TRIGEMINAL SUBNUCLEUS CAUDALIS BY PRIOR EXPOSURE TO ENDOTOXIN 査読

    K. Okamoto, D. F. Bereiter, A. Tashiro, D. A. Bereiter

    NEUROSCIENCE   159 ( 2 )   787 - 794   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Endotoxin-induced uveitis (EIU) is a common animal model for anterior uveitis in humans that causes long-term changes in trigeminal brain stem neurons. This study used c-fos immunohistochemistry to assess the effects of different routes of administration of endotoxin on activation of trigeminal brain stem neurons produced by ocular surface stimulation. A single dose of endotoxin (lipopolysaccharide (LPS)) given to male rats by systemic (i.p., 1 mg/kg) or intraocular (ivt, 20 mu g) routes increased the number of Fos-positive neurons in rostral (trigeminal subnucleus interpolaris/subnucleus transition (Vi/Vc)) and caudal portions of trigeminal subnucleus caudalis (trigeminal subnucleus caudalis/upper cervical spinal cord transition (VC/C(1-2))) by 20% mustard oil (MO) applied to the ocular surface 7 days, but not at 2 days, after LPS compared with naive rats. l.c.v. (20 mu g) LPS did not affect MO-evoked Fos. To determine if the pattern of enhanced Fos expression after systemic LPS also depended on the nature of the ocular surface stimulus, additional groups received ocular stimulation by 10% histamine or dry eye conditions. Seven days, but not 2 days, after i.p. LPS both histamine- and dry eye-evoked Fos was increased at the Vi/Vc transition, while smaller effects were seen at other regions. These results suggested that EIU modulation of trigeminal brain stem neuron activity was mediated mainly by peripheral actions of LPS. Enhancement of Fos at the Vi/Vc region after MO, histamine and dry eye conditions supports the hypothesis that this region integrates innocuous as well as noxious sensory information, while more caudal portions of Vc process mainly nociceptive signals from the eye. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Activation of ERK in the locus coeruleus following acute noxious stimulation 査読

    Hiroki Imbe, Keiichiro Okamoto, Tomohiro Donishi, Shintaro Kawai, Keisuke Enoki, Emiko Senba, Akihisa Kimura

    BRAIN RESEARCH   1263   50 - 57   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In the present study, the activation of extracellular signal-regulated kinase (ERK) in the locus coeruleus (LC) following injection of formalin or complete Freund's adjuvant (CFA) into the rat hindpaw was examined in order to clarify the mechanisms underlying the dynamic changes in the descending pain modulatory system after acute noxious stimulation or chronic inflammation. In naive rats there were few phospho-extracellular signal-regulated kinase-immunoreactive (p-ERK-IR) neurons in the LC. Formalin-, CFA- and saline-injections induced an increase in p-ERK-IR in the LC. The number of p-ERK-IR neurons in the LC in the formalin group was significantly higher than those in all other groups from 5 min to 1 h after the injection (p<0.05). CFA injection induced only a transient significant increase in the number of p-ERK-IR neurons and there was no significant difference in the number of p-ERK-IR neurons between the CFA and saline groups. At 5 min after formalin injection, almost all p-ERK-IR neurons in the LC were tyrosine hydroxylase (TH) -positive. These findings suggest that activation of ERK in the LC is induced by acute noxious stimulation, such as formalin injection, but not by CFA-induced chronic inflammation. The activation of ERK in the LC may be involved in the plasticity of the descending pain modulatory systems following acute noxious stimulation. (C) 2009 Elsevier B.V. All rights reserved.

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  • Morphine modulation of temporomandibular joint-responsive units in superficial laminae at the spinomedullary junction in female rats depends on estrogen status 査読

    A. Tashiro, K. Okamoto, D. A. Bereiter

    EUROPEAN JOURNAL OF NEUROSCIENCE   28 ( 10 )   2065 - 2074   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The influence of analgesic agents on neurons activated by stimulation of the temporomandibular joint (TMJ) region is not well defined. The spinomedullary junction [trigeminal subnucleus caudalis (Vc)/C(1-2)] is a major site of termination for TMJ sensory afferents. To determine whether estrogen status influences opioid-induced modulation of TMJ units, the classical opioid analgesic, morphine, was given to ovariectomized (OvX) rats and OvX rats treated for 2 days with low-dose (LE2) or high-dose (HE2) 17 beta-estradiol-3-benzoate. Under thiopental anesthesia, TMJ units in superficial and deep laminae at the Vc/C(1-2) junction were activated by injection of ATP (1 mm) directly into the joint space. In superficial laminae, morphine inhibited evoked activity in units from OvX and LE2 rats in a dose-related and naloxone-reversible manner, whereas units from HE2 rats were not inhibited. By contrast, in deep laminae, morphine reduced TMJ-evoked unit activity similarly in all groups. Morphine reduced the background activity of units in superficial and deep laminae and resting arterial pressure similarly in all groups. Morphine applied to the dorsal surface of the Vc/C(1-2) junction inhibited all units independently of E2 treatment. Quantitative polymerase chain reaction and immunoblots revealed a similar level of expression for mu-opioid receptors at the Vc/C(1-2) junction in LE2 and HE2 rats. These results indicated that estrogen status differentially affected morphine modulation of TMJ unit activity in superficial, but not deep, laminae at the Vc/C(1-2) junction in female rats. The site(s) for estrogen influence on morphine-induced modulation of TMJ unit activity was probably outside the medullary dorsal horn.

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  • ESTRADIOL REPLACEMENT MODIFIES c-fos EXPRESSION AT THE SPINOMEDULLARY JUNCTION EVOKED BY TEMPOROMANDIBULAR JOINT STIMULATION IN OVARIECTOMIZED FEMALE RATS 査読

    K. Okamoto, D. F. Bereiter, R. Thompson, A. Tashiro, D. A. Bereiter

    NEUROSCIENCE   156 ( 3 )   729 - 736   2008年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The influence of estradiol (E2) treatment on temporomandibular joint (TMJ) nociceptive processing in the caudal trigeminal sensory brain stem complex was assessed in ovariectomized female rats by quantitative Fos-immunore-activity (Fos-LI). After 2 days of daily injections of high (HE2) or low (LE2) dose E2 rats were anesthetized and the small fiber excitant, mustard oil (MO, 0-20%), was injected into the TMJ and after 2 h brains were processed for Fos-LI. TMJ-evoked Fos-Ll in laminae I-II at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C1-2) junction and the dorsal paratrigeminal region (dPa5) was significantly greater in HE2 than LE2 rats, while Fos-LI produced at the ventral trigeminal interpolaris/caudalis transition region (Vi/Vc(vl)) was similar. E2 treatment also modified the influence of N-methyl-D-aspartate (NMDA) and AMPA receptor antagonists on TMJ-evoked Fos-LI. The NMDA antagonist, MK-801, dose-dependently reduced the Fos-Ll response at the Vc/C1-2 junction in HE2 rats, while only high dose MK-801 was effective in LE2 rats. MK801 reduced equally the Fos-LI response at the Vi/Vc transition in both groups, while only minor effects were seen at the dPa5 region. The AMPA receptor antagonist, NBQX, reduced Fos-LI at the VC/C1-2 and Vi/Vc(vl) regions in HE2 rats, while only high dose NBQX was effective in LE2 rats. NBQX did not reduce Fos-LI at the dPa5 region in either group. These results suggest that estrogen status plays a significant role in TMJ nociceptive processing at the Vc/C1-2 junction mediated, in part, through ionotropic glutamate receptor-dependent mechanisms. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Descending facilitation in chronic stress and chronic pain state 査読

    Emiko Senba, Hiroki Imbe, Keiichiro Okamoto

    Japanese Journal of Neuropsychopharmacology   28 ( 1 )   29 - 35   2008年2月

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    記述言語:日本語  

    The spino-thalamic tract consists of two systems
    the lateral system terminates in the somato-sensory cortex, and participates in the sensory discrimination of pain, and the medial system terminates in the anterior cingulated cortex (ACC) and insular cortex (IC) to mediate affective components of pain. Persistent pain induces plastic changes in cortical neurons, especially in the ACC and IC. Activation of these neurons is transmitted to the periaqueductal gray and rostroventromedial medulla (RVM) (descending pain control system). This system has long been considered to exert descending inhibition, but recent studies revealed that it also causes facilitation in certain pathological conditions. A variety of stressful stimuli have been shown to affect pain sensitivity. We demonstrated that chronic restraint stress induced thermal hyperalgesia in rats, in which phosphorylated ERK and levels of tryptophan hydroxylase, a key enzyme of 5-HT production, were increased in the RVM. 5HT released from the bulbospinal neurons may exert facilitatory effects on spinal nociceptive processing probably through 5HT3 receptors. Patients suffering chronic pain originating from deep tissues, such as temporo-mandibular disorder, fibromyalgia, or low back pain, often complain of pain and tenderness in various parts of the body. We injected complete Freund's adjuvant into a temporo-mandibular joint of rats unilaterally, and then injected 5% formalin into the ipsilateral or contralateral masseter muscle 2 weeks later. Pain-related behavior and neuronal activation in the spinal trigeminal nucleus were enhanced on both sides compared to those in non-inflammatory controls. Systemic enhancement of pain and hyperalgesia induced by unilateral joint inflammation may have been caused by the central sensitization and descending facilitation.

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  • Activation of ERK in the rostral ventromedial medulla is involved in hyperalgesia during peripheral inflammation 査読

    Hiroki Imbe, Akihisa Kimura, Keiichiro Okamoto, Tomohiro Donishi, Fumiko Aikawa, Emiko Senba, Yasuhiko Tamai

    BRAIN RESEARCH   1187   103 - 110   2008年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We have previously shown that the extracellular signal-regulated kinase (ERK) is activated in the rostral ventromedial medulla (RVM) during peripheral inflammation. In the present study, the relationship between ERK signaling in the RVM and pain hypersensitivity was investigated in the rat. Microinjection of U0126, a mitogen-activated protein kinase kinase inhibitor, into the RVM decreased phosphorylated ERK at 7 h after complete Freund's adjuvant (CFA) injection into the hindpaw. The U0126 microinjection also attenuated thermal hyperalgesia in the ipsilateral hindpaw at 24 h after CFA injection. The ipsilateral paw withdrawal latency in the U0126 group (67.9%+/- 5.3% vs. baseline, n = 7) was significantly longer than that in the control group (52.0%+/- 3.6% vs. baseline, n = 8). These findings suggest that activation of ERK in the RVM contributes to thermal hyperalgesia. during peripheral inflammation. (c) 2007 Elsevier B.V. All rights reserved.

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  • Differential effects of estradiol on encoding properties of TMJ units in laminae I and V at the spinomedullary junction in female rats 査読

    A. Tashiro, K. Okamoto, S. B. Milam, D. A. Bereiter

    JOURNAL OF NEUROPHYSIOLOGY   98 ( 6 )   3242 - 3253   2007年12月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Differential effects of estradiol on encoding properties of TMJ units in laminae I and V at the spinomedullary junction in female rats. J Neurophysiol 98: 3242-3253, 2007. First published October 10, 2007; doi:10.1152/jn.00677.2007. To determine whether estrogen status modulated dorsal horn neural activity relevant to temporomandibular joint ( TMJ) processing single units were recorded in superficial and deep laminae at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C1-2) junction of ovariectomized (OvX) female rats under barbiturate anesthesia after 17 beta-estradiol (E2) treatment for 2 days. E2 dose-dependently enhanced the response to intra-TMJ stimulation by adenosine triphosphate (ATP) of neurons classified as nociceptive specific (NS), but not wide dynamic range (WDR), in superficial laminae. ATP caused similar responses among NS and WDR neurons from deep laminae in all groups. By contrast, the cutaneous receptive field areas of WDR, but not NS, units in superficial and deep laminae were enlarged in high E2-treated (HE2) compared with low E2-treated (LE2) females. Units from untreated or vehicle-treated male rats displayed responses similar to those of LE2 females. TMJ units in superficial laminae from females were more likely to receive convergent cutaneous input and respond to jaw movement than males, independent of E2 treatment. Western blot analysis revealed similar levels of P2X2 and P2X3 receptor protein in Vc/C1-2 or trigeminal ganglion samples in all groups. Immunohistochemistry revealed dense terminal labeling for P2X3 receptors in superficial laminae and moderate labeling in deep laminae at the Vc/C1-2 junction. These data indicated a significant linkage between estrogen status and the magnitude of articular input evoked by ATP from TMJ neurons in the superficial laminae at the Vc/C1-2 junction, whereas estrogenic modulation of TMJ neurons in deep laminae affected only the convergent input from overlying facial skin.

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  • Activation of central 5HT2A receptors reduces the craniofacial nociception of rats 査読

    K. Okamoto, H. Imbe, A. Kimura, T. Donishi, Y. Tamai, E. Senba

    NEUROSCIENCE   147 ( 4 )   1090 - 1102   2007年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    We assessed the contribution of central 5HT2A receptors to the craniofacial tissue nociception in naive male rats. First, we tested whether activation of central 5HT2A receptors affected nociceptive neural activities recorded from superficial laminae of the trigeminal subnucleus caudalis (Vc)/upper cervical spinal cord junction (Vc/C2) region. Two types of units, such as deep-nociceptive or skin-wide dynamic range (WDR) units were identified from extracellular recordings. Topical administration of 5HT2A receptor agonist, (+/-)-2,5-dimethoxy-4-iodoamphetamine (DOI) onto the Vc/C2 region significantly reduced deep-nociceptive unit discharges evoked by formalin injection into the masseter muscle. Noxious pinch stimulation to the facial skin-evoked skin-WDR unit discharges was significantly reduced by topical administration of 0.1 mg/rat DOI onto the Vc/C2 region. Second, we tested whether i.c.v. administration of DOI affected Fos-like immunoreactivity (-LI) evoked by formalin injection into the masseter muscle. Fos-LI was significantly induced mainly at the ventrolateral (vl) area of trigeminal subnucleus interpolaris (Vi)/Vc junction (vl-Vi/Vc) region and Vc/C2 region in vehicle-treated rats. Formalin-evoked Fos-LI was significantly reduced in laminae I-II of the Vc/C2, but not vl-Vi/Vc region after i.c.v. administration of DOI. Finally, orofacial nocifensive behavioral activities evoked by formalin injection into the masseter muscle were significantly reduced by intracisternal administration of DOI. These results suggest that 5HT2A receptors in the Vc/C2 region mediate antinociceptive effects in the craniofacial nociception. (C) 2007 Published by Elsevier Ltd on behalf of IBRO.

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  • Efferent connections of the ventral auditory area in the rat cortex: implications for auditory processing related to emotion 査読

    A. Kimura, T. Donishi, K. Okamoto, H. Imbe, Y. Tamai

    EUROPEAN JOURNAL OF NEUROSCIENCE   25 ( 9 )   2819 - 2834   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    In the rat auditory cortex, ventral (VA) and posterodorsal (PD) areas are the two major auditory fields that receive thalamic afferents from the dorsal division of the medial geniculate body (MGD). VA and PID are presumed to serve distinct functions in tandem as the pair of major cortical recipients of extralemniscal thalamic inputs. To deduce the functional significance of VA, efferent connections of VA were examined with the anterograde tracer biocytin. VA lies primarily in the ventral margin of area Tel and represents frequencies primarily < 15 kHz [Donishi, T., Kimura, A., Okamoto, K. & Tamai, Y. (2006) Neuroscience, 141, 1553-1567.) Biocytin was iontophoretically injected into cortical regions which were defined as VA based on histological location, auditory response and thalamocortical connectivity. Anterograde labelling revealed two important aspects of cortical projections. First, VA sent a projection to a well-confined region in the caudal end of the insular cortex (Ins) pivotal for fear memory formation during aversive conditioning. Second, VA sent parallel projections to cortical regions that probably comprise the other nonprimary auditory fields, including PD. The results suggest that VA relays auditory input from the MGD to the Ins for affective memory formation and at the same time dispatches the auditory signal, which may represent emotional content, to the remaining nonprimary auditory fields. PD is assumed to play a pivotal role in auditory spatial processing for directed attention (Kimura et al, 2004). As the counterpart of PD, VA is assumed to give rise to another major stream of cortical information processing, most probably related to emotion.

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  • Effects of peripheral inflammation on activation of p38 mitogen-activated protein kinase in the rostral ventromedial medulla 査読

    Hiroki Imbe, Keiichiro Okamoto, Fumiko Aikawa, Akihisa Kimura, Tomohiro Donishi, Yasuhiko Tamai, Yasutomo Iwai-Liao, Emiko Senba

    BRAIN RESEARCH   1134 ( 1 )   131 - 139   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In the present study, the activation of p38 mitogen-activated protein kinase (p38 MAPK) in the rostral ventromedial medulla (RVM) following the injection of complete Freund's adjuvant (CFA) into the rat hindpaw was examined in order to clarify the mechanisms underlying the dynamic changes in the descending pain modulatory system after peripheral inflammation. Phospho-p38 MAPK-immunoreactive (p-p38 MAPKAR) neurons were observed in the nucleus raphe magnus (NRM) and nucleus reticularis gigantocellularis pars alpha (GiA). Inflammation induced the activation of p38 MAPK in the RVM, with a peak at 30 min after the injection of CFA into the hindpaw, which lasted for 1 h. In the RVM, the number of p-p38 MAPK-IR neurons per section in rats killed at 30 min after CFA injection (19.4 +/- 2.0) was significantly higher than that in the naive group (8.4 +/- 2.4) [p < 0.05]. At 30 min after CFA injection, about 40% of p-p38 MAPKAR neurons in the RVM were serotonergic neurons (tryptophan hydroxylase, TPH, positive) and about 70% of TPH-IR neurons in the RVM were p-p38 MAPK positive. The number of p-p38 MAPK- and TPH-double-positive RVM neurons in the rats with inflammation was significantly higher than that in naive rats [p < 0.05]. These findings suggest that inflammation-induced activation of p38 MAPK in the RVM may be involved in the plasticity in the descending pain modulatory system following inflammation. (c) 2006 Elsevier B.V. All rights reserved.

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  • Contribution of peripheral 5-HT2A or 5-HT3 receptors to Fos expression in the trigeminal spinal nucleus produced by acute injury to the masseter muscle during persistent temporomandibular joint inflammation in rats 査読

    K. Okamoto, A. Kimura, T. Donishi, H. Imbe, Y. Nishie, H. Matsushita, Y. Tamai, E. Senba

    NEUROSCIENCE   143 ( 2 )   597 - 606   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    We investigated the contribution of peripheral 5-HT2A or 5-HT3 receptors to Fos expression in the trigeminal spinal nucleus (VSP) following acute masseter muscle injury in male rats with or without temporomandibular joint (TMJ) inflammation persisting for 7 days. TMJ inflammation was evoked by an injection of complete Freund's adjuvant (CFA). Two hours after formalin injection into the masseter muscle produced Fos-like immunoreactivity (Fos-LI) in several regions of the VSP and upper cervical spinal cord (C2), such as ventrolateral (vI) area of the trigeminal subnucleus caudalis (Vc)/subnucleus interpolaris (Vi) transition (vI-Vi/Vc), paratrigeminal nucleus (dPa5), middle portion of the Vc (mid-Vc) and Vc/C2 transition (Vc/C2) regions in both groups. Significant increases in the number of Fos-LI were observed in these areas in CFA group compared with non-CFA group. TMJ inflammation alone did not induce a significant level of Fos-LI in the VSP. In order to assess the effect of antagonizing 5-HT2A or 5-HT3 receptors on formal in-induced Fos-LI, rats were pre-treated with local (masseter muscle) administration of ketanserin or tropisetron (0.01, 0.1 mg/rat) 20 min prior to formalin injection. In CFA group, these antagonists given locally reduced the Fos-LI response in the laminae I-II at the mid-Vc and Vc/C2 regions. These antagonists reduced the Fos-LI response in the dPa5, but not in the vI-Vi/Vc region. The Fos-LI response was not affected by i.v. administration of ketanserin (0.01, 0.1 mg/rat) or tropisetron (0.01 mg/rat). In non-CFA group, these antagonists given locally did not reduce the Fos-LI response. These results suggest that peripheral 5-HT2A and 5-HT3 receptors contribute to nociceptive processing in the masseter muscle in TMJ inflammatory conditions. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Persistent monoarthritis of the temporomandibular joint region enhances nocifensive behavior and lumbar spinal Fos expression after noxious stimulation to the hindpaw in rats 査読

    K Okamoto, A Kimura, T Donishi, H Imbe, K Goda, K Kawanishi, Y Tamai, E Senba

    EXPERIMENTAL BRAIN RESEARCH   170 ( 3 )   358 - 367   2006年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Effects of persistent temporomandibular joint (TMJ) inflammation on nociceptive responses of remote bodily areas of the rat were investigated. Monoarthritis of the TMJ region was evoked by the injection of complete Freund's adjuvant (CFA) into the left TMJ region. Rats without injection of CFA into the TMJ region served as controls (non-CFA group). Time spent on licking behavior evoked by the injection of formalin into the left hindpaw and withdrawal thresholds of mechanical stimulation to both sides of the hindpaw were measured during TMJ inflammation for 3 weeks. Furthermore, expression of Fos protein in the lumbar dorsal horn was immunohistochemically investigated following the injection of formalin into the hindpaw during TMJ inflammation. Formalin-evoked nocifensive behavioral activities were significantly enhanced at 10 and 14 days after CFA injection in the late phase, while the withdrawal threshold to mechanical stimulation was significantly decreased bilaterally at 8, 10 and 14 days after CFA injection. Both formalin-evoked licking behavior and mechanical withdrawal thresholds to bilateral hindpaw at 21 days after CFA injection were similar to those in the non-CFA group. The number of Fos-positive neurons in the lumbar dorsal horn ipsilateral to the formalin injection at 1 and 7 days after CFA injection into the TMJ were similar to those in the non-CFA group; however, those were significantly increased in the laminae I-II and V-VI of the lumbar dorsal horn at 14 days after CFA injection. TMJ inflammation for 7 and 14 days alone produced a small number of Fos-expressing neurons in the lumbar dorsal horn. These results provide evidence that persistent unilateral inflammation of the TMJ region causes an increase in behavioral hyperalgesia of the hindpaw, which is attributed to the modulation of neural activities, in part, in the lumbar dorsal horn, likely mediated by supraspinal neural mechanisms.

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  • 炎症後の吻側延髄腹内側部におけるp38MAPKの活性化

    井辺 弘樹, 岡本 圭一郎, 岡村 友玄, 隈部 俊二, 中塚 美智子, 相川 文子, 岩井 康智, 仙波 恵美子

    解剖学雑誌   81 ( Suppl. )   236 - 236   2006年3月

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    記述言語:英語   出版者・発行元:(一社)日本解剖学会  

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  • "Ventral" area in the rat auditory cortex: A major auditory field connected with the dorsal division of the medial geniculate body 査読

    T. Donishi, A. Kimura, K. Okamoto, Y. Tamai

    NEUROSCIENCE   141 ( 3 )   1553 - 1567   2006年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The rat auditory cortex is made up of multiple auditory fields. A precise correlation between anatomical and physiological areal extents of auditory fields, however, is not yet fully established, mainly because non-primary auditory fields remain undetermined. In the present study, based on thaiamocortical connection, electrical stimulation and auditory response, we delineated a non-primary auditory field in the cortical region ventral to the primary auditory area and anterior auditory field. We designated it as "ventral" area after its relative location. At first, based on anterograde labeling of thalamocortical projection with blocytin, ventral auditory area was delineated as a main cortical terminal field of thalamic afferents that arise from the dorsal division of the medial geniculate body. Cortical terminal field (ventral auditory area) extended into the ventral margin of temporal cortex area 1 (Te1) and the dorsal part of temporal cortex area 3, ventral (Te3V), from 3.2-4.6 mm posterior to bregma. Electrical stimulation of the dorsal division of the medial geniculate body; evoked epicortical field potentials confined to the comparable cortical region. On the basis of epicortical field potentials evoked by pure tones, best frequencies were further estimated at and around the cortical region where electrical stimulation of the dorsal division of the medial geniculate body evoked field potentials. Ventral auditory area was found to represent frequencies primarily below 15 kHz, which contrasts with our previous finding that the posterodorsal area, the other major recipient of the dorsal division of the medial geniculate body; projection, represents primarily high frequencies (> 15 kHz). The posterodorsal area is thought to play a pivotal role in auditory spatial processing [Kimura A, Donishi T, Okamoto K, Tamai Y (2004) Efferent connections of "posterodorsal" auditory area in the rat cortex: implications for auditory spatial processing. Neuroscience 128:399419]. The ventral auditory area, as the other main cortical region that would relay auditory input from the dorsal division of the medial geniculate body to higher cortical information processing, could serve an important extralemniscal function in tandem with the posterodorsal area. The results provide insight into structural and functional organization of the rat auditory cortex. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Endotoxin-induced uveitis causes long-term changes in trigeminal subnucleus caudalis neurons 査読

    DA Bereiter, K Okamoto, A Tashiro, H Hirata

    JOURNAL OF NEUROPHYSIOLOGY   94 ( 6 )   3815 - 3825   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Endotoxininduced uveitis (EIU) is commonly used in animals to mimic ocular inflammation in humans. Although the peripheral aspects of EIU have been well studied, little is known of the central neural effects of anterior eye inflammation. EIU was induced in male rats by endotoxin or lipopolysaccharide (LPS, 1 mg/kg ip) given 2 or 7 days earlier. Neurons responsive to mechanical stimulation of the ocular surface were recorded under barbiturate anesthesia at the trigeminal subnucleus interpolaris/ caudalis (Vi/Vc) transition and subnucleus caudalis/cervical cord (Vc/C1) junction, the main terminal regions for corneal nociceptors. Two days after LPS, Vc/C1 units had reduced responses to histamine, nicotine, and CO2 gas applied to the ocular surface, whereas unit responses were increased 7 days after LPS. Those units with convergent cutaneous receptive fields at Vc/C1 were enlarged 7 days after LPS. Units at the Vi/Vc transition also had reduced responses to histamine and CO2 2 days after LPS but no enhancement was seen at 7 days. Tear volume evoked by CO2 was reduced 2 days after LPS and returned toward control values by 7 days, whereas CO 2-evoked eye blinks were normal at 2 days and increased 7 days after LPS. These results indicate that a single exposure to endotoxin causes long-term changes in the excitability of second-order neurons responsive to noxious ocular stimulation. The differential effects of EIU on tear volume and eye blink lend further support for the hypothesis that ocular-sensitive neurons at the Vi/Vc transition and Vc/C1 junction regions mediate different aspects of pain during intraocular inflammation.

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  • Effects of peripheral inflammation on activation of ERK in the rostral ventromedial medulla 査読

    H Imbe, K Okamoto, T Okamura, S Kumabe, M Nakatsuka, F Aikawa, Y Iwai-Liao, E Senba

    BRAIN RESEARCH   1063 ( 2 )   151 - 158   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    In the present study, the activation of extracellular signal-regulated kinase (ERK) in the rostral ventromedial medulla (RVM) following the injection of complete Freund's adjuvant (CFA) into the rat hindpaw was examined in order to clarify the mechanisms underlying the dynamic changes in the descending pain modulatory system after peripheral inflammation. Phospho-extracellular signal-regulated kinase-immunoreactive (p-ERK-IR) neurons were observed in the nucleus raphe magnus (NRM) and nucleus reticularis gigantocellularis pars alpha (GiA). Inflammation induced the activation of ERK in the RVM, with a peak at 7 h after the injection of CFA into the hindpaw and a duration of 24 h. In the RVM, the number of p-ERK-IR neurons per section in rats killed at 7 h after CFA injection (14.2 +/- 1.7) was significantly higher than that in the control group (4.5 +/- 0.9) [P < 0.01]. At 7 h after CFA injection, about 60% of p-ERK-IR neurons in the RVM were serotonergic neurons. The percentage of RVM serotonergic neurons that are also p-ERK positive in the rats with inflammation (20.5% +/- 2.3%) was seven times higher than that in control rats (2.7% +/- 1.4%) [P < 0.01]. These findings suggest that inflammation-induced activation of ERK in the RVM may be involved in the plasticity in the descending pain modulatory system following inflammation. (C) 2005 Elsevier B.V. All rights reserved.

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  • Effect of persistent monoarthritis of the temporomandibular joint region on acute mustard oil-induced excitation of trigeminal subnucleus caudalis neurons in male and female rats 査読

    DA Bereiter, K Okamoto, DF Bereiter

    PAIN   117 ( 1-2 )   58 - 67   2005年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The effect of persistent inflammation of the temporomandibular (TMJ) region on Fos-like immunoreactivity (Fos-LI) evoked by acute noxious stimulation of the same or opposite TMJ was assessed in male and cycling female rats. Two weeks after inflammation of the TMJ by complete Freund's adjuvant (CFA, 25 mu g) the selective small fiber excitant, mustard oil (MO, 20%), was injected into the arthritic or opposite TMJ under barbiturate anesthesia. MO stimulation of the arthritic TMJ increased Fos-LI ipsilateral, but not contralateral, to MO compared to naive subjects in superficial laminae at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C-2) junction independent of sex hormone status. Unexpectedly, MO stimulation of the opposite TMJ in arthritic rats also produced a greater Fos-LI response ipsilateral to MO than naive animals. Fos-LI produced in the dorsal paratrigeminal region (dPa5) and Vc/C-2 junction after MO stimulation of the normal TMJ was significantly greater in proestrous than diestrous females or male monoarthritic rats. In contrast to naive animals, Fos-LI was produced in deep laminae at the Vc/C-2 junction ipsilateral to MO in CFA-treated animals independent of the site of prior CFA inflammation or sex hormone status. These results indicated that persistent monoarthritis of the TMJ region enhanced the excitability of trigeminal brainstem neurons to subsequent TMJ injury that occurred bilaterally in multiple regions of the lower trigeminal brainstem complex and depended on sex hormone status. (c) 2005 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

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  • Differential modulation of TMJ neurons in superficial laminae of trigeminal subnucleus caudalis/upper cervical cord junction region of male and cycling female rats by morphine 査読

    K Okamoto, A Tashiro, H Hirata, DA Bereiter

    PAIN   114 ( 1-2 )   203 - 211   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Sex differences in the cellular responses to morphine were examined in an animal model of temporomandibular joint (TMJ) pain. TMJ-responsive neurons were recorded in the superficial laminae at the trigeminal subnucleus caudalis/upper cervical cord (Vc/C-2) junction region, the initial site of synaptic integration for TMJ afferents, in male and cycling female rats under barbiturate anesthesia. Unit activity was evoked by local injection of bradykinin into the TMJ capsule at 30 min intervals and the effects of morphine sulfate (0.03-3 mg/kg, i.v.) were assessed by a cumulative dose regimen. Morphine caused a dose-related inhibition of bradykinin-evoked unit activity in males and diestrous females in a naloxone-reversible manner, while evoked unit activity in proestrous females was not reduced. The apparent sex hormone-related aspect of morphine analgesia was selective for evoked unit activity, since the spontaneous activity of TMJ units was reduced similarly in all groups, while the convergent cutaneous receptive field area of TMJ units did not change in any group. These results were consistent with the hypothesis that sex hormone status interacts with pain control systems to modify neural activity at the level of the Vc/C-2 junction region relevant for TMD pain. (c) 2004 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

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  • The role of peripheral 5HT2A and 5HT1A receptors on the orofacial formalin test in rats with persistent temporomandibular joint inflammation 査読

    K Okamoto, H Imbe, A Tashiro, A Kimura, T Donishi, Y Tamai, E Senba

    NEUROSCIENCE   130 ( 2 )   465 - 474   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The role of peripheral serotonin (5HT) 2A and 5HT1A receptors on the orofacial nocifensive behavioral activities evoked by the injection of formalin into the masseter muscle was evaluated in the rats with persistent temporomandibular joint (TMJ) inflammation evoked by Complete Freund's Adjuvant (CFA). The orofacial nocifensive behavioral activities evoked by the injection of formalin into masseter muscle were significantly enhanced at I day (CFA day 1 group) or 7 days (CFA day 7 group) during TMJ inflammation. Pretreatment with local administration of 5HT2A receptor antagonist, ketanserin (0.01, 0.1 mg/rat) into the masseter muscle or systemic administration of ketanserin via i.p. injection (1 mg/kg) reduced the orofacial nocifensive behavioral activities of the late phase evoked by formalin injection into masseter muscle on the side of TMJ inflammation (CFA day 7 group). However, local (0.001-0.1 mg/rat) or systemic (1 mg/kg) administration of 5HT1A receptor antagonist, propranolol, into masseter muscle did not produce the antinoci- ceptive effect in CFA day 7 group. Moreover, local administration of ketanserin (0.1 mg) or propranolol (0.1 mg) into masseter muscle did not inhibit nocifensive orofacial behavior in rats without TMJ inflammation. These data suggest that persistent TMJ inflammation causes the elevation of the orofacial nocifensive behavior, and peripheral 5HT2A receptors play an important role in mediating the deep craniofacial tissue nociception in rats with TMJ inflammation. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Topography of projections from the primary and non-primary auditory cortical areas to the medial geniculate body and thalamic reticular nucleus in the rat 査読

    A Kimura, T Donishi, K Okamoto, Y Tamai

    NEUROSCIENCE   135 ( 4 )   1325 - 1342   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The functional significance of parallel and redundant information processing by multiple cortical auditory fields remains elusive. A possible function is that they may exert distinct corticofugal modulations on thalamic information processing through their parallel connections with the medial geniculate body and thalamic reticular nucleus. To reveal the anatomical framework for this function, we examined corticothalamic projections of tonotopically comparable subfields in the primary and non-primary areas in the rat auditory cortex. Biocytin was injected in and around cortical area Tel after determining best frequency at the injection site on the basis of epicortical field potentials evoked by pure tones. The rostral part of area Tel (primary auditory area) and area temporal cortex, area 2, dorsal (Te2D) (posterodorsal auditory area) dorsal to the caudal end of area Tel, which both exhibited high best frequencies, projected to the ventral zone of the ventral division of the medial geniculate body. The caudal end of area Tel (auditory area) and the rostroventral part of area Tel (a part of anterior auditory field), which both exhibited low best frequencies, projected to the dorsal zone of the ventral division of the medial geniculate body. In contrast to the similar topography in the projections to the ventral division of the medial geniculate body, collateral projections to the thalamic reticular nucleus terminated in the opposite dorsal and ventral zones of the lateral and middle tiers of the nucleus in each pair of the tonotopically comparable cortical subfields. In addition, the projections of the non-primary cortical subfields further arborized in the medial tier of the thalamic reticular nucleus. The results suggest that tonotopically comparable primary and non-primary subfields in the auditory cortex provide corticofugal excitatory effects to the same part of the ventral division of the medial geniculate body. On the other hand, corticofugal inhibition via the thalamic reticular nucleus may operate in different parts of the ventral division of the medial geniculate body or different thalamic nuclei. The primary and non-primary cortical auditory areas are presumed to subserve distinct gating functions for auditory attention. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.

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  • Central serotonin 3 receptors play an important role in the modulation of nociceptive neural activity of trigeminal subnucleus caudalis and nocifensive orofacial behavior in rats with persistent temporomandibular joint inflammation 査読

    K Okamoto, A Kimura, T Donishi, H Imbe, E Senba, Y Tamai

    NEUROSCIENCE   135 ( 2 )   569 - 581   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The role of central serotonin 3 receptors on neural activities recorded from superficial laminae of trigeminal subnucleus caudalis/upper cervical spinal cord junction region was investigated using rats with (Complete Freund's Adjuvant day 7 group) or without (non-Complete Freund's Adjuvant group) persistent temporomandibular joint inflammation evoked by Complete Freund's Adjuvant for 7 days. We identified two types of units, Deep-wide dynamic range units and Skin-wide dynamic range units from extracellular recordings. Deep-wide dynamic range units have mechanoreceptive fields in the deep cranlofacial tissues including masseter muscle but do not have cutaneous mechanoreceptive fields. Deep-wide dynamic range unit discharges evoked by the formalin injection into masseter muscle were significantly enhanced in the late phase in Complete Freund's Adjuvant day 7 group. Discharges of Skin-wide dynamic range units evoked by the noxious pinch stimulation to facial skin in Complete Freund's Adjuvant day 7 group were significantly enhanced compared with those in non-Complete Freund's Adjuvant group. Topical administration of central serotonin 3 receptor antagonist, tropisetron, onto trigeminal subnucleus caudalis/upper cervical spinal cord junction region significantly reduced both formalin-evoked Deep-wide dynamic range unit and pinch-evoked Skin-wide dynamic range unit discharges in non-Complete Freund's Adjuvant and Complete Freund's Adjuvant day 7 groups significantly. The inhibitory effects of tropisetron on pinch-evoked Skin-wide dynamic range unit discharges were prolonged in Complete Freund's Adjuvant day 7 group compared with those in non-Complete Freund's Adjuvant group. The role of central serotonin 3 receptors in trigeminal subnucleus caudalis/upper cervical spinal cord junction region was also tested by oro-facial formalin test in Complete Freund's Adjuvant day 7 group. Intracisternal administration of tropisetron decreased the orofacial nocifensive behavior in the late phase evoked by the injection of formalin into the masseter muscle. These results suggest that central serotonin 3 receptors in trigeminal subnucleus caudalis/upper cervical spinal cord junction region are involved in mediating pronociceptive effects in both superficial and deep craniofacial tissues nociception during persistent temporomandibular joint inflammation. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.

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  • The effects of acute and chronic restraint stress on activation of ERK in the rostral ventromedial medulla and locus coeruleus 査読

    H Imbe, S Murakami, K Okamoto, Y Iwai-Liao, E Senba

    PAIN   112 ( 3 )   361 - 371   2004年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Extracellular signal-regulated kinase (ERK) is a key molecule in numerous cellular and physiological processes in the CNS. Exposure to stressors causes substantial effects on the perception and response to pain. The rostral ventromedial medulla (RVM) and the locus coeruleus (LC) play crucial roles in descending pain modulation system. In the present study, the activation of ERK in the RVM and the LC in rats following acute and chronic restraint stress was examined in order to characterize the mechanisms underlying stress induced analgesic and hyperalgesic responses. Rats were stressed by restraint 6 h daily for 3 weeks. The acute and chronic restraint stresses produced analgesic and hyperalgesic reactions, respectively, to thermal stimuli applied to the tail. The phospho-ERK-immunoreactive (p-ERK-IR) neurons were observed in the nucleus raphe magnus (NRM), nucleus reticularis gigantocellularis pars alpha (GiA) and LC. In the RVM, the number of p-ERK-IR neurons per section in the 3-week restraint rats (14.3+/-1.2) was significantly higher than that in the control rats (8.9+/-0.7) [P<0.01]. About 75% of p-ERK-IR neurons in the RVM in the 3-week restraint rats were serotonergic neurons. Protein levels of tryptophan hydroxylase were significantly increased in the RVM region in the 3-week restraint rats. On the other hand, the chronic restraint stress significantly decreased p-ERK-IR in the LC [P<0.05]. These findings suggest that chronic restraint stress-induced activation of ERK in the RVM and the suppression in the LC may be involved in the modulation of the pain threshold by chronic stress. (C) 2004 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

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  • Blockade of peripheral 5HT3 receptor attenuates the formalin-induced nocifensive behavior in persistent temporomandibular joint inflammation of rat 査読

    K Okamoto, H Imbe, A Tashiro, S Kumabe, E Senba

    NEUROSCIENCE LETTERS   367 ( 2 )   259 - 263   2004年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    The role of peripheral 5HT3 receptors in the orofacial nocifensive behavior induced by the injection of formalin into masseter muscle was evaluated. The behavioral activities evoked by the formalin injection exhibited a biphasic response in the rats with or without temporomandibular joint (TMJ) inflammation (CFA group or non-CFA group). The orofacial nocifensive behavioral activity was enhanced after TMJ inflammation. Systemic administration of tropisetron, 5HT3 receptor antagonist, reduced the nocifensive behavioral activities in the late phase of orofacial formalin test in CEA group, but not in non-CFA group. Local administration of tropisetron into the masseter muscle in CFA group, but not in non-CFA group also attenuated the behavioral activities in the late phase. Unexpectedly, low dose of local tropisetron reduced the nocifensive behavioral activities in the early phase of orofacial formalin test in CFA group. These data suggest that induction of TMJ inflammation causes the elevation of the orofacial nocifensive behavioral activities evoked by formalin injection into masseter muscle, and that peripheral 5HT3 receptors may play a critical role in nociception and the transmission of orofacial pain. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

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  • Increase of galanin-like immunoreactivity in rat hypothalamic arcuate neurons after peripheral nerve injury 査読

    H Imbe, T Abe, K Okamoto, M Sato, H Ito, S Kumabe, E Senba

    NEUROSCIENCE LETTERS   368 ( 1 )   102 - 106   2004年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI IRELAND LTD  

    Galanin and galanin receptors are widely distributed within the central nervous system, and may play important roles in pain signaling and modulation. In the present study, we examined the galanin immunoreactivity (IR) in the hypothalamus and the amygdala following peripheral nerve injury. Four weeks after the operation, the ipsilateral mechanical threshold in the spared nerve injury (SNI) group (0.87 +/- 0.33 g) was significantly lower than that in the sham group (12.53 +/- 3.41 g; P < 0.05). In the SNI group, the number of galanin-IR neurons per section in the arcuate nucleus (Arc) of the hypothalamus was 10.2 +/- 1.7, significantly higher than that in the sham group (5.6 +/- 1.0; P < 0.05). These data suggest that the galanin-ergic neurons in the Arc may be involved in the functional modulation of descending pain modulation system following peripheral nerve injury. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

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  • A novel class of neurons at the trigeminal subnucleus interpolaris/caudalis transition region monitors ocular surface fluid status and modulates tear production 査読

    H Hirata, K Okamoto, A Tashiro, DA Bereiter

    JOURNAL OF NEUROSCIENCE   24 ( 17 )   4224 - 4232   2004年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC NEUROSCIENCE  

    Reflex tears are produced by many conditions, one of which is drying of the ocular surface. Although peripheral neural control of the lacrimal gland is well established, the afferent pathways and properties of central premotor neurons necessary for this reflex are not known. Male rats under barbiturate anesthesia were used to determine whether neurons at the ventral trigeminal subnucleus interpolaris-caudalis (Vi/Vc) transition or the trigeminal subnucleus caudalis-cervical cord (Vc/C1) junction region in the lower brainstem were necessary for tears evoked by noxious chemical stimulation (CO2 pulses) or drying of the ocular surface. Both the Vi/Vc transition and Vc/C1 junction regions receive a dense direct projection from corneal nociceptors. Synaptic blockade of the Vi/Vc transition, but not the Vc/C1 junction, by the GABA(A) receptor agonist muscimol inhibited CO2-evoked tears. Glutamate excitation of the Vi/Vc transition, but not the Vc/C1 junction, increased tear volume. Single units recorded at the Vi/Vc transition, but not at the Vc/C1 junction, were inhibited by wetting and excited by drying the ocular surface. Nearly all moisture-sensitive Vi/Vc units displayed an initial inhibitory phase to noxious concentrations of CO2 followed by delayed excitation and displayed an inhibitory surround receptive field from periorbital facial skin. Drying of the ocular surface produced many Fos-positive neurons at the Vi/Vc transition, but not at the Vc/C1 junction. This is the first report of a unique class of moisture-sensitive neurons that exist only at the ventral Vi/Vc transition, and not at more caudal portions of Vc, that may underlie fluid homeostasis of the ocular surface.

    DOI: 10.1523/JNEUROSCI.0381-04.2004

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  • Topography of corticothalamic projections from the auditory cortex of the rat 査読

    M Hazama, A Kimura, T Donishi, T Sakoda, Y Tamai

    NEUROSCIENCE   124 ( 3 )   655 - 667   2004年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Corticothalamic projections from cortical auditory field to the medial geniculate body (MG) in the rat were systematically examined by making small injections of biocytin in cortical area Te1. All injections, confined to 400 mum in diameter, resulted in two projections terminating in the ventral (MGV) and dorsal divisions (MGD) of the MG. The projections to the MGV were evidently topographic. The rostral and caudal portions of area Te1 projected to the ventromedial and dorsolateral parts of the MGV, respectively, forming narrow bands of terminal axons that extended in the mediolateral direction in the coronal plane of the MGV. The minimum dorsoventral width of the bands ranged approximately from 100 to 300 mum. Besides, the more rostral portion of area Te1 tended to project to the more rostral side of the MGV. The projections to the MGD consistently arborized in its ventral margin made up of the deep dorsal nucleus of the MGD. A similar weak topography along the rostrocaudal direction was observed in the projections to the MGD. Large terminals were occasionally found in the MGD after the injections involving cortical layer V. The distribution of large terminals also appeared topographic along with small terminals that were the major component of labeling. Collaterals of labeled axons produced slabs of terminal field in the thalamic reticular nucleus, which also exhibited a weak topography of distribution. These results provide insights into the structural basis of corticofugal modulations related to the tonotopic organizations in the cortex and MG. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.

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  • Galectin-1 is involved in the potentiation of neuropathic pain in the dorsal horn 査読

    H Imbe, K Okamoto, T Kadoya, H Horie, E Senba

    BRAIN RESEARCH   993 ( 1-2 )   72 - 83   2003年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Galectin-1 is one of the endogenous-galactoside-binding lectins, suggested to be involved in a variety of functions, such as neurite outgrowth, synaptic connectivity, cell proliferation and apoptosis. This protein is expressed in the dorsal root ganglion (DRG) and the spinal cord in the developing and adult rats, especially intensely in small DRG neurons. In the present study, we examined whether galectin-1 is colocalized with TrkA or c-Ret mRNA in small DRG neurons and the effect of axotomy on the expression of galectin-1 in the spinal cord. About 20% of the DRG neurons showed intense galectin-l-immunoreactivity (IR). Of the intensely galectin-1-IR DRG neurons, 93.9% displayed c-Ret mRNA positive signals. On the other hand, only 6.8% displayed TrkA mRNA positive signals. Galectin-1-IR was increased in the dorsal horn at 1 to 2 weeks after axotomy. Intrathecal administration of anti-recombinant human galectin-1 antibody (anti-rhGAL-1 Ab) partially but significantly attenuated the upregulation of substance P receptor (SPR) in the spinal dorsal horn and the mechanical hypersensitivity induced by the peripheral nerve injury. These data suggest that endogenous galectin-1 may potentiate neuropathic pain after the peripheral nerve injury at least partly by increasing SPR in the dorsal horn. (C) 2003 Elsevier B.V. All rights reserved.

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  • GABA(A) receptor activation modulates corneal unit activity in rostral and caudal portions of trigeminal subnucleus caudalis 査読

    H Hirata, K Okamoto, DA Bereiter

    JOURNAL OF NEUROPHYSIOLOGY   90 ( 5 )   2837 - 2849   2003年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Corneal nociceptors terminate at the trigeminal subnucleus interpolaris/caudalis (Vi/Vc) transition and subnucleus caudalis/upper cervical spinal cord (Vc/C1) junction regions of the lower brain stem. The aims of this study were to determine if local GABA(A) receptor activation modifies corneal input to second-order neurons at these regions and if GABA(A) receptor activation in one region affects corneal input to the other region. In barbiturate-anesthetized male rats, corneal nociceptors were excited by pulses of CO2 gas, and GABA(A) receptors were activated by microinjections of the selective agonist muscimol. Local muscimol injection at the site of recording inhibited all Vi/Vc and Vc/C1 units tested and was reversed partially by bicuculline. To test for ascending intersubnuclear communication, muscimol injection into the caudal Vc/C1 junction, remote from the recording site at the Vi/Vc transition, inhibited the evoked response of most corneal units, although some neurons were enhanced. Injection of the nonselective synaptic blocking agent, CoCl2, remotely into the Vc/C1 region inhibited the evoked response of all Vi/Vc units tested. To test for descending intersubnuclear communication, muscimol was injected remotely into the rostral Vi/Vc transition and enhanced the evoked activity of all corneal units tested at the caudal Vc/C1 junction. These results suggest that GABA(A) receptor mechanisms play a significant role in corneal nociceptive processing by second-order trigeminal brain stem neurons. GABA(A) receptor mechanisms act locally at both the Vi/Vc transition and Vc/C1 junction regions to inhibit corneal input and act through polysynaptic pathways to modify corneal input at multiple levels of the trigeminal brain stem complex.

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  • Response properties of TMJ units in superficial laminae at the spinomedullary junction of female rats vary over the estrous cycle 査読

    K Okamoto, H Hirata, S Takeshita, DA Bereiter

    JOURNAL OF NEUROPHYSIOLOGY   89 ( 3 )   1467 - 1477   2003年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Neurons responsive to stimulation of the temporomandibular joint (TMJ) region were recorded from superficial laminae at the trigeminal subnucleus caudalis/ upper cervical cord (VC/C-2) junction region of cycling female rats under barbiturate anesthesia. To determine if receptive field (RF) properties or sensitivity to algesic chemicals of TMJ units vary over the estrous cycle, animals were selected from proestrous (high estrogen) or early diestrous (low estrogen) stages. More than 90% of TMJ units from each group received convergent nociceptive input [wide dynamic range (WDR) or nociceptive specific (NS)-like] from facial skin. The cutaneous high-threshold RF areas of WDR units from proestrous rats were 30% larger than diestrous units, while RF areas of NS units were similar. Bradykinin (BK, 0.1-10 muM) injection into the TMJ region excited a high percentage of units (>80% of total) from both groups in a dose-related manner. However, BK-evoked response magnitude (R-mag, +140%) and duration (+64%) were greater for proestrous than diestrous units. Both WDR and NS-like TMJ units of proestrous females displayed enhanced BK-evoked R-mag values and response duration. Glutamate or mustard oil excitation of TMJ units was not affected by stage of the estrous cycle. Several TMJ units from proestrous and diestrous females were activated antidromically from the contralateral posterior thalamus, indicating that projection and nonprojection units were included in the sample population. These results were consistent with the hypothesis that factors related to stage of the estrous cycle modify the processing of deep craniofacial inputs by superficial dorsal horn neurons at the spinomedullary junction, a key region for the initial integration of sensory signals from the TMJ.

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  • 5-HT2A receptor subtype in the peripheral branch of sensory fibers is involved in the potentiation of inflammatory pain in rats 査読

    K Okamoto, H Imbe, Y Morikawa, M Itoh, M Sekimoto, K Nemoto, E Senba

    PAIN   99 ( 1-2 )   133 - 143   2002年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    One of the major serotonin (5-HT) receptor subtypes expressed in the rat dorsal root ganglion (DRG) neurons is the 5-HT2A receptor. We have previously shown that 5-HT2A receptors in the peripheral sensory terminals are responsible for 5-HT-induced pain and hyperalgesia. In the present study, we characterized neurons expressing 5-HT2A receptors in the rat DRG neurons by means of in situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and behavioral tests. In situ hybridization on consecutive sections revealed that 5-HT2A receptor mRNA is colocalized with calcitonin-gene related peptide (CGRP) mRNA (100/104; 96.2%) but not with c-Ret mRNA (1/115; 0.9%). Signals for 5-HT2A receptor mRNA were found in 9.4 +/- 2.2% of normal DRG (L5) neurons, most of which were small to medium in size. Four days of complete Freund's adjuvant-induced inflammation of the hindpaw doubled the incidence of 5-HT2A receptor mRNA-expressing neurons to 19.3 +/- 2.8%. The level of 5-HT2A receptor mRNA in DRGs of normal and various pathological conditions was then determined by RT-PCR. The level was up-regulated by peripheral inflammation, but not by axotomy or chronic constriction of the peripheral nerve. Systemic administration of 5-HT2A receptor antagonist (Sarpogrelate HCI) produced analgesic effects on thermal hyperalgesia caused by peripheral inflammation, but failed to attenuate thermal hyperalgesia in chronic constriction injury model. These findings suggest that 5-HT2A receptors are mainly expressed in CGRP-synthesizing small DRG neurons and may be involved in the potentiation of inflammatory pain in the periphery. (C) 2002 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0304-3959(02)00070-2

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  • A novel analgesic compound OT-7100 attenuates nociceptive responses in animal models of inflammatory and neuropathic hyperalgesia: A possible involvement of adenosinergic anti-nociception 査読

    T Yasuda, K Okamoto, T Iwamoto, S Miki, N Yoshinaga, S Sato, K Noguchi, E Senba

    JAPANESE JOURNAL OF PHARMACOLOGY   87 ( 3 )   214 - 225   2001年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    We studied the effects of OT-7100 (5-n-butyl-7-(3,4,5-trimethoxybenzoylamino)pyrazolo [1,5-a]pyrimidine), a novel analgesic compound, on the inhibitory action of adenosine on the contraction of guinea pig ileum and investigated the effects of OT-7100 on the nociceptive responses in animal models of inflammatory and peripheral neuropathic hyperalgesia and decreases spinal c-Fos expression. OT-7100 at 0.3 - 3 muM significantly enhanced the inhibitory effect of adenosine on the contraction of guinea pig ileum. The efficacy of OT-7100 (1, 3 or 10 mg/kg, p.o.) on hyperalgesia induced by yeast or substance P and in the Bennett model was significantly suppressed by coadministration of the adenosine A(1) antagonist DPCPX (0.01 or 0.1 pmol/animal, i.t.), while OT-7100 without DPCPX significantly increased the nociceptive threshold in each rat model. OT-7100 (3, 10 and 30 mg/kg per day, p.o.) significantly inhibited the mechanical nociceptive threshold in the injured paw in the Chung model. OT-7100 (30 mg/kg, p.o.) significantly decreased the number of Fos-LI neurons in the spinal dorsal horn in the Bennett model. These finding suggest that OT-7100 inhibits hyperalgesia in these animal models possibly by enhancing adenosinergic neurotransmission in the dorsal horn, although we still lack direct evidence for it.

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  • Myofascial flap without skin for intra-oral reconstruction 2: Clinical studies 査読

    Takeshi Wada, Keiichirou Okamoto, Yasuhiro Nakanishi, Hideshi Nakano, Yoshinobu Iwagami, Nobuo Morita

    International Journal of Clinical Oncology   6 ( 3 )   143 - 148   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background. Based on the results of our animal experiment, we evaluated the clinical usefulness of myofascial graft materials in the reconstruction of intra-oral soft-tissue defects. Methods. An axial-pattern or random-pattern myofascial flap was grafted to reconstruct oral soft-tissue defects caused by tumor resection in ten patients. A pectoralis major myofascial flap was employed in four patients, and a platysma myofascial flap was employed in six patients. Results. All flaps survived, and epithelialization progressed gradually from the surrounding incised mucosal margin. Cicatricial contracture of the wound seemed to be mild and the regenerated mucosa was more flexible than skin. Because the myofascial tissue had only raw surfaces, the handling of the flaps in the oral cavity was very flexible. Moreover, cosmetically unsatisfactory scar formation and dysfunction at the donor site were mild. Conclusion. We feel that the myofascial graft procedure is a very useful option for the reconstruction of intra-oral soft-tissue defects, and will soon become a common procedure. We refer to this procedure as the "biological-guided mucosa regeneration (BGMR)" technique.

    DOI: 10.1007/PL00012097

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  • Myofascial flap without skin for intra-oral reconstruction 1: Animal studies 査読

    Takeshi Wada, Keiichirou Okamoto, Yasuhiro Nakanishi, Yoshinobu Iwagami, Nobuo Morita

    International Journal of Clinical Oncology   6 ( 1 )   40 - 44   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background. Although myofascial flaps are already used clinically in intra-oral reconstruction, the source and process of epithelialization remain unclear. Methods. The process of epithelialization of grafted myofascial in the oral cavity was investigated using the latissimus dorsi muscle of Wistar strain rats. Results. The use of myofascial tissue to fill in defects may maintain the space by preventing shrinkage and contracture of the wound, and this myofascial tissue may induce regeneration of the mucosal epithelium. Myofascial itself did not have the potential to epithelialize. Epithelialization progressed gradually along the surface of the granulated myofascial from the surrounding incised mucosal margin of the recipient site to the central portion. Immunohistochemical staining showed that keratinocyte growth factor expressing cells were found mainly in the granulated-myofascial. Within the study period, regenerated epithelium with orthokeratosis consisting of stratified squamous cells was thin and had scanty rete pegs, but it was similar to normal epithelium. Conclusion. Grafted myofascial in the oral cavity may play a space-making role, and induce regeneration of the mucosal epithelium, associated with the production of keratinocyte growth factor.

    DOI: 10.1007/PL00012078

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  • 一次知覚伝達系におけるgalectin-1の局在とその機能について

    井辺 弘樹, 岡本 圭一郎, 森川 吉博, 仙波 恵美子

    PAIN RESEARCH   15 ( 3 )   130 - 130   2000年12月

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    記述言語:日本語   出版者・発行元:日本疼痛学会  

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  • 神経損傷後の後根神経節におけるGM1 ganglioside発現とCholera toxin B標識の変化

    仙波 恵美子, 井辺 弘樹, 岡本 圭一郎, 森川 吉博

    PAIN RESEARCH   15 ( 3 )   131 - 131   2000年12月

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    記述言語:日本語   出版者・発行元:日本疼痛学会  

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  • ラット知覚神経節における5-HT2A及び5-HT3リセプター産生細胞

    岡本 圭一郎, 森川 吉博, 仙波 恵美子

    解剖学雑誌   75 ( 1 )   125 - 125   2000年2月

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    記述言語:英語   出版者・発行元:(一社)日本解剖学会  

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  • 国保橋本市民病院歯科口腔外科における顎顔面骨骨折症例の臨床統計的観察 査読

    岡本 圭一郎, 松平 淳, 川島 稔弘

    和歌山医学   49 ( 2 )   113 - 117   1998年5月

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    記述言語:日本語   出版者・発行元:和歌山医学会  

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  • 診断・治療に苦慮した口唇炎に対しトラニラスト(リザベン)の著効した1例

    川島 稔弘, 岡本 圭一郎, 坂本 忠幸

    診療と新薬   35 ( 1 )   59 - 61   1998年1月

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    記述言語:日本語   出版者・発行元:(株)医事出版社  

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  • 口腔症状を契機に発見された白血病症例の臨床的観察 査読

    岡本 圭一郎, 和田 健, 大亦 哲司

    日本口腔科学会雑誌   47 ( 1 )   108 - 114   1998年1月

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    記述言語:日本語   出版者・発行元:(NPO)日本口腔科学会  

    DOI: 10.11277/stomatology1952.47.108

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  • 犬による顔面部咬傷の2例と文献的考察 査読

    岡本 圭一郎, 川島 稔弘

    和歌山医学   48 ( 3 )   407 - 410   1997年8月

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    記述言語:日本語   出版者・発行元:和歌山医学会  

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  • 口腔顎顔面外傷の入院症例における臨床統計的観察 査読

    川島 稔弘, 岡本 圭一郎, 湯浅 祥司

    和歌山医学   48 ( 1 )   65 - 68   1997年2月

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    記述言語:日本語   出版者・発行元:和歌山医学会  

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  • 下顎枝矢状分割後,顎動脈領域に発現した動脈瘤の1例 査読

    岡本 圭一郎, 森田 展雄, 大亦 哲司, 坂本忠幸

    日本口腔外科学会雑誌   42 ( 12 )   1221 - 1223   1996年12月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔外科学会  

    DOI: 10.5794/jjoms.42.1221

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  • 高校生集団における顎関節症の自覚症状に関する疫学的検討 査読

    河野 聡子, 森田 展雄, 宮田 和幸, 岡本圭一郎, 坂本忠幸

    日本顎関節学会雑誌   8 ( 2 )   435 - 442   1996年9月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • 口腔の脂肪腫の臨床病理学的検討 過去10年間の当科症例と文献的考察 査読

    岡本 圭一郎, 和田 健, 川島 稔弘

    日本口腔外科学会雑誌   42 ( 3 )   270 - 276   1996年3月

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    記述言語:日本語   出版者・発行元:(公社)日本口腔外科学会  

    DOI: 10.5794/jjoms.42.270

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  • 食道癌発生後,6年間に口腔粘膜の4ヶ所から発生した多発癌の1症例と文献的考察 査読

    岡本 圭一郎, 和田 健, 原田 昌和, 坂本忠幸

    日本口腔腫瘍学会誌   7 ( 4 )   354 - 358   1995年12月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔腫瘍学会  

    DOI: 10.5843/jsot.7.354

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書籍等出版物

  • 歯科衛生士国家試験対策1

    岡本圭一郎( 担当: 分担執筆)

    医歯薬出版  2018年 

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  • 口腔顔面痛の診断と治療

    岡本圭一郎( 担当: 分担執筆 ,  範囲: 神経障害性疼痛)

    医歯薬出版  2017年9月 

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MISC

  • 酒粕GABAによるストレス誘発痛の抑制機構の解明 招待

    岡本圭一郎

    公益財団法人飯島唐十郎記念食品科学振興財団   34   30 - 37   2019年8月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(その他)  

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  • 顎関節痛を調節する下行性疼痛制御系について 招待 査読

    黒瀬雅之, 岡本圭一郎

    日本顎関節学会雑誌   31   149 - 158   2019年

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    担当区分:最終著者, 責任著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

    添付ファイル: 日本顎関節学会雑誌31.149-.2019.pdf

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  • 反復的な強制水泳がもたらす心理的ストレスは、吻側延髄腹内側部(RVM)における顎顔面領域での侵害情報伝達を変調する

    黒瀬 雅之, 長谷川 真奈, 清水 志保, 中谷 暢佑, 藤井 規孝, 佐藤 義英, 山村 健介, 岡本 圭一郎

    Journal of Oral Biosciences Supplement   2018   467 - 467   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 情動ストレスによるセロトニン機構の変調は大縫線核および青斑核の咬筋侵害応答を増大させる

    清水 志保, 中谷 暢佑, 黒瀬 雅之, 長谷川 真奈, 高木 律男, 山村 健介, 岡本 圭一郎

    Journal of Oral Biosciences Supplement   2018   257 - 257   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 日本酒によるストレス誘発性の咬筋侵害応答の軽減効果は日本酒含有エタノールの直接作用ではない

    中谷 暢佑, 柿原 嘉人, 清水 志保, 黒瀬 雅之, 佐伯 万騎男, 高木 律男, 山村 健介, 岡本 圭一郎

    Journal of Oral Biosciences Supplement   2018   260 - 260   2018年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 三叉神経支配領域への侵害刺激を受容する吻側延髄腹側部ニューロンの電気生理学的特性

    長谷川 真奈, 黒瀬 雅之, 岡本 圭一郎, 中谷 暢佑, 清水 志保, 山村 健介, 藤井 規孝

    新潟歯学会雑誌   48 ( 1 )   59 - 59   2018年6月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • 強制水泳ストレスは顎顔面領域における吻側延髄腹側部での侵害受容を変調する

    黒瀬 雅之, 長谷川 真奈, 岡本 圭一郎, 中谷 暢佑, 清水 志保, 藤井 規孝, 山村 健介

    新潟歯学会雑誌   48 ( 1 )   58 - 59   2018年6月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • 多チャネル容量性触圧センサを用いた頬粘膜の咀嚼時接触面圧測定

    長谷川 真奈, 黒瀬 雅之, 岡本 圭一郎, 渡辺 啓介, 新井 啓介, 佐藤 秋彦, 家亀 里佳, 山村 健介, 山田 好秋

    日本咀嚼学会雑誌   27 ( 2 )   115 - 115   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本咀嚼学会  

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  • 多チャネル容量性触圧センサを用いた咀嚼時の頬粘膜による接触面圧測定

    長谷川 真奈, 黒瀬 雅之, 岡本 圭一郎, 清水 志保, 中谷 暢佑, 山村 健介, 藤井 規孝, 高木 律男, 山田 好秋

    Journal of Oral Biosciences Supplement   2017   297 - 297   2017年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • セロトニン再取り込み阻害薬は繰り返しストレスによる咬筋の侵害受容反応の増強を抑制する

    中谷 暢佑, 黒瀬 雅之, 清水 志保, 柿原 嘉人, 木口 哲郎, 長谷川 真奈, 佐伯 万騎男, 高木 律男, 山村 健介, 岡本 圭一郎

    Journal of Oral Biosciences Supplement   2017   463 - 463   2017年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 顎顔面皮膚および深部組織への侵害刺激がもたらす吻側廷髄腹側部ニューロンの応答特性

    黒瀬 雅之, 長谷川 真奈, 岡本 圭一郎, 清水 志保, 中谷 暢佑, 藤井 規孝, 高木 律男, 山田 好秋, 山村 健介

    Journal of Oral Biosciences Supplement   2017   456 - 456   2017年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 『酒は百薬の長』の根拠を科学的に解明するストレス誘発性の咬筋侵害受容反応に対する日本酒の影響について

    岡本 圭一郎, 中谷 暢佑, 黒瀬 雅之, 柿原 嘉人, 木口 哲郎, 長谷川 真奈, 藤井 規孝, 佐伯 万騎男, 高木 律男, 山村 健介

    Journal of Oral Biosciences Supplement   2017   462 - 462   2017年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • セロトニン再取り込み阻害薬は繰り返しストレスによる咬筋の侵害受容反応の増強を抑制する

    中谷 暢佑, 岡本 圭一郎, 高木 律男

    日本顎関節学会雑誌   29 ( Suppl. )   136 - 136   2017年7月

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    記述言語:日本語   出版者・発行元:(一社)日本顎関節学会  

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  • 大気圧センサを用いた健常者の嚥下時咽頭内圧変化の測定

    長谷川 真奈, 岡本 圭一郎, 黒瀬 雅之, 山田 好秋, 山村 健介

    日本咀嚼学会雑誌   26 ( 2 )   98 - 98   2016年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本咀嚼学会  

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  • 口唇随意運動時の脳活動の変化 fNIRSによる分析

    黒瀬 雅之, 岡本 圭一郎, 長谷川 真奈, 中谷 暢佑, 藤井 規孝, 山田 好秋, 佐藤 義英, 山村 健介, 増田 裕次

    Journal of Oral Biosciences Supplement   2016   515 - 515   2016年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 大気圧センサを用いた咽頭内圧測定システムによる嚥下機能の評価

    長谷川 真奈, 岡本 圭一郎, 黒瀬 雅之, 山田 好秋, 藤井 規孝, 山村 健介

    Journal of Oral Biosciences Supplement   2016   318 - 318   2016年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 持続性の顎関節炎が両側性の咬筋に痛覚過敏を引き起こす中枢メカニズム

    岡本 圭一郎, 黒瀬 雅之, 中谷 暢佑, 長谷川 真奈, 藤井 規孝, 高木 律夫, 山村 健介

    Journal of Oral Biosciences Supplement   2016   504 - 504   2016年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • 大気圧センサを用いた嚥下時咽頭内圧変化の測定

    長谷川 真奈, 山田 好秋, 黒瀬 雅之, 岡本 圭一郎, 藤井 規孝, 山村 健介

    新潟歯学会雑誌   46 ( 1 )   49 - 49   2016年7月

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    記述言語:日本語   出版者・発行元:新潟歯学会  

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  • 口腔生理学分野の研究最前線Frontier in the field of Oral Physiology 乾燥に"潤い"をもたらすメカニズムの解明

    黒瀬 雅之, 八田 あずさ, 岡本 圭一郎, Meng Ian D, 山村 健介

    Journal of Oral Biosciences Supplement   2015   112 - 112   2015年9月

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    記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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  • A comment on experimental occlusal interference induces long-term masticatory muscle hyperalgesia in rats by Cao et al. 査読

    David A. Bereiter, Keiichiro Okamoto, Akimasa Tashiro, Donald R. Nixdorf

    PAIN   148 ( 3 )   519 - 519   2010年3月

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    記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.pain.2010.01.003

    Web of Science

    PubMed

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  • ストレスと痛み 脳による疼痛の制御 査読

    仙波 恵美子, 井辺 弘樹, 岡本 圭一郎

    日本神経精神薬理学雑誌   28 ( 1 )   29 - 35   2008年2月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:(一社)日本神経精神薬理学会  

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  • Contribution of peripheral 5HT2A or 5HT3 receptors to Fos expression in the trigeminal spinal nucleus (VSP) produced by the masseter muscle injury of rats

    Keiichiro Okamoto, Akihisa Kimura, Tomohiro Donishi, Yasuhiko Tamai

    NEUROSCIENCE RESEARCH   55   S188 - S188   2006年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER IRELAND LTD  

    Web of Science

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  • 【痛みシグナルの制御機構と最新治療エビデンス】 疼痛の生理機構と分子メカニズム セロトニン受容体サブタイプと慢性痛

    岡本 圭一郎, 仙波 恵美子

    医学のあゆみ   211 ( 5 )   370 - 374   2004年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)   出版者・発行元:医歯薬出版(株)  

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  • ラット知覚神経節における5-HT_<2A>リセプター産生細胞

    岡本 圭一郎, 関本 征史, 根本 清光, 森川 吉博, 仙波 恵美子

    Pain research : the journal of the Japanese Society for the Study of Pain = 日本疼痛学会誌   14 ( 3 )   1999年12月

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    記述言語:日本語  

    CiNii Article

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講演・口頭発表等

  • 米発酵エキスが象牙芽細胞様細胞(KN-3)のタンパク質発現におよぼす影響について

    岡本圭一郎, 柿原嘉人, 佐伯万騎男

    第40回歯科薬物療法学会 (横浜市)  2020年6月 

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    開催年月日: 2020年6月 - 2020年7月

    記述言語:日本語   会議種別:口頭発表(一般)  

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  • トレッドミル走は社会的敗北ストレスが引き起こす咬筋痛を軽減する

    長谷川真奈, 岡本圭一郎

    第25回日本口腔顔面痛学会学術大会  2020年11月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • 繰り返しトレッドミル走は社会的敗北ストレスによる顎顔面領域の侵害応答を抑制する

    長谷川真奈, 岡本圭一郎, 藤井規孝, 山村健介

    第62回歯科基礎医学会学術大会  2020年9月 

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    会議種別:口頭発表(一般)  

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  • Talking about Japanese Culture ~Is Sake the best of a hundred medicine? ~ 招待

    岡本圭一郎

    International Collabotrative Symposium on Development of Human Resources in Practical Oral Health and Treatment  2020年2月 

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    記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(指名)  

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  • 顎顔面部への侵害刺激による吻側延髄腹内側部(RVM)の興奮性は繰り返しストレスによって変化する

    長谷川真奈, 黒瀬雅之, 岡本圭一郎, 藤井規孝, 山村健介

    三叉神経系領域の感覚ー運動統合機構研究会(軽井沢)  2019年11月 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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  • 酒粕エキスが象牙芽細胞様細胞の分化におよぼす影響

    K Okamoto, Y Kakihara, L Thant, A Washio, C Kitamura, K Yamamura, M Saeki

    第61回歯科基礎医学会  2019年10月 

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    記述言語:日本語   会議種別:ポスター発表  

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  • Sake (Japanese Rice Wine) reduces Fos responses in the medullary dorsal horn under psychophysical stress conditions in the rats. 招待 国際会議

    岡本圭一郎

    Store Kro Club, Dunkeld, Scotland, UK.  2019年6月 

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    記述言語:英語   会議種別:口頭発表(招待・特別)  

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  • Psychological stress modulates On- and Off-cell activity in the rostral ventromedial medulla. 国際会議

    Masayuki Kurose, Mana Hasegawa, Yosuke Nakatani, Shiho Shimizu, Noritaka Fujii, Yoshihide Satoh, Kensuke Yamamura, Keiichiro Okamoto

    FAOPS 2019 (Kobe Japan)  2019年3月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Effect of repeated and acute psychophysical stress on masseter muscle nociception in the trigeminal subnucleus caudalis region of the rats. 国際会議

    Shiho Shimizu, Yoshito Kakihara, Mayumi Taiyoji, Yosuke Nakatani, Nobuyuki Ikeda, Makio Saeki, Ritsuo Takagi, Kensuke Yamamura, Keiichiro Okamoto

    FAOPS 2019 (Kobe, Japan)  2019年3月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Brain Mechanisms for enhanced masseter muscle nociception under psychophysical stress conditions. 招待 国際会議

    岡本圭一郎

    2019 International Niigata-Taiwan Universities Collaborative Dental Research Symposium(Taipei, Taiwan)  2019年3月 

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    記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

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  • 第23回うま味研究助成成果発表会 招待

    岡本圭一郎

    うま味研究会(東京)  2019年1月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • 日本酒は百薬の長を科学的に解明する方法 招待

    岡本圭一郎

    ライスエキスポ 2018 (新潟市)  2018年11月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • Modulatory effects of psychological stress on descending pain controls 招待

    Oral Neuroscience 2018 (大阪)  2018年10月 

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    記述言語:英語   会議種別:口頭発表(招待・特別)  

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  • ストレスと日本酒 招待

    岡本圭一郎

    日本酒学シンポジウム(新潟市)  2018年8月 

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    記述言語:日本語   会議種別:メディア報道等  

    添付ファイル: スキャン.jpeg

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  • 教育講演 顎関節痛を調節する下行性疼痛制御機構 招待

    岡本圭一郎

    日本口腔顔面痛学会(北九州市)  2018年7月 

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    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • Psychophysical Stress Enhances Orofacial Nociception in the Rostral Ventromedial Medulla 国際会議

    Shiho Shimizu, Yosuke Nakatani, Masayuki Kurose, Mana Hasegawa, Nobuyuki Ikeda, Noritaka Fujii, Ritsuo Takagi, Kensuke Yamamura, Keiichiro Okamoto

    2018年7月  IADR

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    記述言語:英語   会議種別:ポスター発表  

    開催地:London  

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  • Japanese Rice Wine, Sake, inhibits Stress-Induced Jaw Muscle Nociception 国際会議

    Keiichiro Okamoto, Yosuke Nakatani, Shiho Shimizu, Masayuki Kurose, Yoshito Kakihara, Makio Saeki, Ritsuo Takagi, Kensuke Yamamura

    IADR  2018年7月  IADR

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    記述言語:英語   会議種別:ポスター発表  

    開催地:London  

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  • Japanese Rice Wine (Sake) reduced enhanced masseter muscle nociception under repeated forced swim stress conditions in rats

    Keiichiro Okamoto, Yosuke Nakatani, Shiho Shimizu, Masayuki Kurose, Yoshito kakihara, Ritsuo Takagi, Kensuke Yamamura

    第95回日本生理学会大会  2018年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:高松  

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  • Modulatory effect of psychophysical stress on orofacial nociception at the rostral ventromedial medulla in the rats 国際会議

    Masayuki Kurose, Mana Hasegawa, Shiho Shimizu, Yousuke Nakatani, Noritaka Fujii, Kensuke Yamamura, Keiichiro Okamoto

    2018年  Society of Neuroscience

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    記述言語:英語   会議種別:ポスター発表  

    開催地:San Diego  

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  • 反復的な強制水泳がもたらす心理的ストレスは、吻側廷髄腹側部(RVM)における顎顔面領域での侵害情報伝達を変調する

    黒瀬雅之, 長谷川真奈, 清水志保, 中谷暢佑, 藤井規孝, 佐藤義英, 山村健介, 岡本圭一郎

    第60回 歯科基礎医学会学術大会  2018年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:塩尻  

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  • ディープラーニングによる 「食べ易さ」「飲み込み易さ」 判定の可能性

    黒瀬 雅之, 長谷川 真奈, 岡本 圭一郎, 山田 好秋, 山村 健介, 佐藤 大祐

    日本咀嚼学会第29回学術大会  2018年  日本咀嚼学会

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    記述言語:日本語  

    開催地:松本  

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  • うまみは減塩につながるか? 塩味応答性の弧束核ニューロンに対するうまみ刺激の影響

    渡部清人, 黒瀬雅之, 岡本圭一郎, 山村健介

    日本咀嚼学会第29回学術大会  2018年  日本咀嚼学会

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    記述言語:日本語  

    開催地:松本  

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  • 強制水泳ストレスは顎顔面領域における侵害受容を変調する

    黒瀬雅之, 長谷川真奈, 岡本圭一郎, 中谷暢佑, 清水志保, 藤井規孝, 山村健介

    第51回新潟歯学会総会  2018年  新潟歯学会

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    記述言語:日本語  

    開催地:新潟  

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  • 三叉神経支配領域への侵害刺激を受容する吻側延髄腹側部ニューロンの電気生理学的特性

    長谷川真奈, 黒瀬雅之, 岡本圭一郎, 中谷暢佑, 清水志保, 山村健介, 藤井規孝

    第51回新潟歯学会総会  2018年  新潟歯学会

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    記述言語:日本語  

    開催地:新潟  

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  • Psychophysical stress increases nociceptive neural activities evoked by masseter muscle injury in the rostral ventral medulla (RVM) in rats. 国際会議

    Shiho Shimizu, Yosuke Nakatani, Masayuki Kurose, Kensuke Yamamura, Ritsuo Takagi, Keiichiro Okamoto

    第95回日本生理学会大会  2018年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:高松  

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  • 顎顔面皮膚および深部組織への侵害刺激がもたらす吻側廷髄腹側部ニューロンの応答特性

    黒瀬雅之, 長谷川真奈, 岡本圭一郎, 清水志保, 中谷暢佑, 藤井規孝, 高木律男, 山田好秋, 山村健介

    第59回 歯科基礎医学会学術大会  2017年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:塩尻  

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  • Bilateral Increases in ERK Activation at the Spinomedullary Junction Region and Orofacial Nocifensive Behavioral Activity by Masseter Muscle Stimulation During Temporomandibular Joint Inflammation in the Rat 国際会議

    Keiichiro Okamoto, Masayuki Kurose, Hiroki Imbe, Emiko Senba, Kensuke Yamamura

    16th World Congress on Pain  2017年  IASP

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    記述言語:英語   会議種別:ポスター発表  

    開催地:_浜  

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  • 大気圧センサを用いた健常者の嚥下時咽頭内圧変化の測定

    長谷川真奈, 山田好秋, 黒瀬雅之, 岡本圭一郎, 山村健介

    日本咀嚼学会第27回学術大会  2017年  日本咀嚼学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:広島  

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  • 多チャネル容量性触圧センサを用いた頬粘膜の咀嚼時接触面圧測定

    長谷川真奈, 黒瀬雅之, 岡本圭一郎, 渡辺啓介, 新井啓介, 佐藤秋彦, 家亀里佳, 山村健介, 山田好秋

    日本咀嚼学会第28回学術大会  2017年  日本咀嚼学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:東京  

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  • Effect of repeated and acute psychophysical stress on masseter muscle nociception in the trigeminal subnucleus caudalis region of the rats 国際会議

    Yosuke Nakatani, Keiichiro Okamoto, Masayuki Kurose, Mana Hasegawa, Noritaka Fujii, Ritsuo Takagi, Kensuke Yamamura

    第94回日本生理学会大会  2017年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:札幌  

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  • 『酒は百薬の長』の根拠を科学的に解明するストレス誘発性の咬筋侵害受容反応に対する日本酒の影響について

    岡本圭一郎, 中谷暢佑, 黒瀬雅之, 柿原嘉人, 木口哲郎, 長谷川真奈, 藤井規孝, 佐伯万騎男, 高木律男, 山村健介

    第59回 歯科基礎医学会学術大会  2017年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:塩尻  

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  • セロトニン再取り込み阻害薬は繰り返しストレスによる咬筋の侵害受容反応の増強を抑制する

    中谷暢佑, 黒瀬雅之, 清水志保, 柿原嘉人, 木口哲郎, 長谷川真奈, 佐伯万騎男, 高木律男, 山村健介, 岡本 圭一郎

    第59回 歯科基礎医学会学術大会  2017年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:塩尻  

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  • 多チャネル容量性触圧センサを用いた咀嚼時の頬粘膜による接触面圧測定

    長谷川真奈, 黒瀬雅之, 岡本圭一郎, 清水志保, 中谷暢佑, 山村健介, 藤井規孝, 高木律男, 山田好秋

    第59回 歯科基礎医学会学術大会  2017年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:塩尻  

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  • 口唇随意運動時の脳活動の変化 fNIRSによる分析

    黒瀬雅之, 岡本圭一郎, 長谷川真奈, 中谷暢佑, 藤井規孝, 山田好秋, 佐藤義英, 山村健介, 増田 裕次

    第58回 歯科基礎医学会学術大会  2016年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:札幌  

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  • 乾燥に"潤い"をもたらすメカニズムの解 明. 招待

    黒瀬雅之, 八田あずさ, 岡本圭一郎, Meng ID, 山, 村健介

    第57回 歯科基礎医学会学術大会  2016年  歯科基礎医学会

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    記述言語:日本語  

    開催地:新潟  

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  • 大気圧センサを用いた咽頭内圧測定システムによる嚥下機能の評価

    長谷川真奈, 岡本圭一郎, 黒瀬雅之, 山田好秋, 藤井 規孝, 山村 健介

    第58回 歯科基礎医学会学術大会  2016年  歯科基礎医学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:札幌  

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  • Visual-feedback を用 いた口唇随意閉鎖運動時の脳活動の変化:fNIRS に よる分析

    黒瀬雅之, 増田裕次, 竹花快恵, 井上正雄, 岡本圭一郎, 山本悠, 山村健介

    第 18 回日本ヒト脳機能マッピング学会  2016年  日本ヒト脳機能マッピング学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:京都  

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  • Cerebral blood flow during adjusting voluntary lip-closing force using the visual feedback 国際会議

    Yuji Masuda, Masayuki Kurose, Yoshie Takehana, Keiichiro Okamoto, Kensuke Yamamura

    第93回日本生理学会大会  2016年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:札幌  

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  • photophobia(羞明)の神経機構 招待

    岡本圭一郎

    第21回日本口腔顔面痛学会学術大会  2016年  日本口腔顔面痛学会学術大会

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    記述言語:日本語  

    開催地:_浜  

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  • Bright light activates trigeminal pathways 招待 国際会議

    Keiichiro Okamoto, Akimasa Tasshiro, Masayuki Kurose, Kensuke Yamamura, David A Bereiter

    第94回日本生理学会大会  2016年  日本生理学会

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    記述言語:英語  

    開催地:札幌  

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  • A new method for the study of velopharyngeal function using digital barometer. 国際会議

    Mana Hasegawa, Keiichiro Okamoto, Masayuki Kurose, Yoshiaki Yamada, Kensuke Yamamura

    第93回日本生理学会大会  2016年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:札幌  

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  • Dry eye sensitizes cold cells to capsaicin-mediated inhibition via TRPV1. 国際会議

    Masayuki Kurose, Azusa Hatta, Keiichiro Okamoto, Kensuke Yamamura, Ian D. Meng

    第93回日本生理学会大会  2016年  日本生理学会

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    記述言語:英語   会議種別:ポスター発表  

    開催地:札幌  

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  • 大気圧センサを用いた健常者の嚥下時咽頭内圧変化の測定

    長谷川真奈, 山田好秋, 黒瀬雅之, 岡本圭一郎, 山村健介

    日本咀嚼学会第27回学術大会  2016年  日本咀嚼学会

     詳細を見る

    記述言語:日本語   会議種別:ポスター発表  

    開催地:広島  

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  • Measurements of Pharyngeal Barometric Pressure During Swallowing in the Healthy Subjects

    Mana Hasegawa, Keiichiro Okamoto, Masayuki Kurose, Takanori Tsujimura, Makoto Inoue, Noritaka Fujii, Yoshiaki Yamada, Kensuke Yamamura

    第22 回日本摂食嚥下リハビリテーション学会学術大会  2016年  日本摂食嚥下リハビリテーション学会

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    記述言語:日本語   会議種別:ポスター発表  

    開催地:新潟  

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  • Bilateral Increases in ERK Activation at the Spinomedullary Junction Region and Orofacial Nocifensive Behavioral Activity by Masseter Muscle Stimulation During Temporomandibular Joint Inflammation in the Rat 国際会議

    Keiichiro Okamoto, Masayuki Kurose, Hiroki Imbe, Emiko Senba, Kensuke Yamamura

    16th World Congress on Pain  2016年  IASP

     詳細を見る

    記述言語:英語   会議種別:ポスター発表  

    開催地:_浜  

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  • 持続性の顎関節炎が両側性の咬筋に痛覚過敏を引き起こす中枢メカニズム

    岡本圭一郎, 黒瀬雅之, 中谷暢佑, 長谷川真奈, 藤井規孝, 高木律男, 山村健介

    第58回 歯科基礎医学会学術大会  2016年  歯科基礎医学会

     詳細を見る

    記述言語:日本語   会議種別:ポスター発表  

    開催地:札幌  

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  • 乾燥に"潤い"をもたらすメカニズムの解 明.

    黒瀬雅之, 八田あずさ, 岡本圭一郎, Meng ID, 山, 村健介

    第57回 歯科基礎医学会学術大会  2015年  歯科基礎医学会

     詳細を見る

    記述言語:日本語  

    開催地:新潟  

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  • photophobia(羞明)の神経機構 招待

    岡本圭一郎

    第21回日本口腔顔面痛学会学術大会  2012年  日本口腔顔面痛学会学術大会

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    記述言語:日本語  

    開催地:_浜  

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  • Repeated treadmill running exercises reduced orofacial hyperalgesia following social defeat stress in mice.

    Mana Hasegawa, Keiichiro Okamoto, Noritaka Fujii, Kensuke Yamamura

    第62回日本基礎歯科医学会総会 

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    記述言語:日本語   会議種別:口頭発表(一般)  

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共同研究・競争的資金等の研究

  • 運動療法がストレス誘発性の顎顔面痛を軽減する脳メカニズム

    2019年4月 - 2022年3月

    日本学術振興会 基盤研究C 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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  • 酒粕トリプトファンが社会的敗北ストレスに起因する痛みと睡眠障害を改善する脳神経機構の解明

    2018年9月 - 2019年8月

    公益財団法人 ひと・健康・未来研究財団 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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  • 介護予防促進のための『むせる』を予測するシステムの開発

    2018年4月 - 2022年3月

    日本学術振興会  基盤研究B 

    山村健介

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    資金種別:競争的資金

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  • 孤束核の塩味応答性神経細胞の興奮性が、うま味刺激によって増大するメカニズムの解明

    2018年4月 - 2020年3月

    うまみ研究会  研究助成 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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  • 酒粕GABAによるストレス誘発性のウツ・痛みの軽減機構の解明

    2018年4月 - 2019年3月

    公益財団法人飯島藤十郎記念食品科学振興財団 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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  • ストレスが顎関節痛を増強させる脳神経メカニズム

    2016年4月 - 2019年3月

    日本学術振興会  基盤研究C 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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  • ラットの三叉神経脊髄路核尾側亜核の5HT2A受容体が顎顔面痛におよぼす影響と性差

    2005年4月 - 2006年3月

    日本学術振興会  若手研究B 

    岡本圭一郎

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    担当区分:研究代表者  資金種別:競争的資金

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担当経験のある授業科目(researchmap)

  • 口腔生理学

    機関名:新潟大学大学院医歯学総合研究科(歯学部歯学科

     詳細を見る

  • 生理学2

    機関名:新潟大学大学院医歯学総合研究科(歯学部歯学科

     詳細を見る

  • 機能生理学

    機関名:新潟大学工学部

     詳細を見る

  • 人体の解剖と生理

    機関名:明倫短期大学・歯科衛生士科

     詳細を見る

  • 人体のしくみ

    機関名:新潟大学大学院医歯学総合研究科 口腔生命福祉学科

     詳細を見る

  • 生理学1

    機関名:新潟大学大学院医歯学総合研究科(歯学部歯学科

     詳細を見る

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担当経験のある授業科目

  • 人体のしくみ

    2019年
    -
    現在
    機関名:新潟大学

  • 口腔生理学

    2018年
    -
    現在
    機関名:新潟大学

  • 神経科学演習ⅡA

    2018年
    機関名:新潟大学

  • 生理学Ⅱ

    2017年
    -
    現在
    機関名:新潟大学

  • 生理学Ⅰ

    2017年
    -
    現在
    機関名:新潟大学

  • 機能生理学

    2017年
    -
    現在
    機関名:新潟大学

  • 神経科学演習ⅠA

    2017年
    機関名:新潟大学

  • 神経科学演習ⅠB

    2017年
    機関名:新潟大学

  • 基本生理学

    2016年
    -
    現在
    機関名:新潟大学

  • 器官生理学

    2016年
    -
    現在
    機関名:新潟大学

  • 基礎科学演習

    2016年
    機関名:新潟大学

  • 生体機能学

    2015年
    -
    現在
    機関名:新潟大学

▶ 全件表示

 

社会貢献活動

  • 新潟大学 日本酒学体験講座 文部科学省

    役割:講師, 運営参加・支援

    日本酒学ことはじめ 日本酒と健康  2019年12月

     詳細を見る

  • 新潟大学 市民公開講座(新潟市)

    役割:講師, 運営参加・支援

    新潟大学  日本酒学ことはじめ 日本酒と健康  2018年12月

     詳細を見る

  • 明倫短期大学 非常勤講師

    役割:講師

    2016年4月 - 現在

     詳細を見る