Updated on 2024/02/02
学士(医学) ( 2007.3 新潟大学 )
全身麻酔薬
Life Science / Neuroscience-general
新潟大学医歯学総合研究科 生体機能調節医学専攻 器官制御医学 助教
2019.4
Niigata University Graduate School of Medical and Dental Sciences Biological Functions and Medical Control Cardiovascular and Vital Control Assistant Professor
2019.4
Niigata University University Medical and Dental Hospital Anesthesiology Assistant Professor
2017.3 - 2019.3
Niigata University University Medical and Dental Hospital Anesthesiology Specially Appointed Assistant Professor
2016.2 - 2017.2
Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord. Reviewed International journal
Yuka Nakamura, Miyuki Kurabe, Mami Matsumoto, Tokiharu Sato, Satoshi Miytashita, Kana Hoshina, Yoshinori Kamiya, Kazuki Tainaka, Hitoshi Matsuzawa, Nobuhiko Ohno, Masaki Ueno
eLife 12 2023.2
Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation Reviewed
Miyuki Kurabe, Mika Sasaki, Kenta Furutani, Hidemasa Furue, Yoshinori Kamiya, Hiroshi Baba
iScience 25 ( 12 ) 105555 - 105555 2022.12
A mechanism of re-sedation caused by remimazolam. Reviewed
Tomohiro Yamamoto, Miyuki Kurabe, Yoshinori Kamiya
Journal of anesthesia 35 ( 3 ) 467 - 468 2021.6
Re-sleeping after reversal of remimazolam by flumazenil. Reviewed
Tomohiro Yamamoto, Miyuki Kurabe, Yoshinori Kamiya
Journal of anesthesia 35 ( 2 ) 322 - 322 2021.4
Serotonin plays a key role in the development of opioid-induced hyperalgesia in mice. Reviewed International journal
Mika Sasaki, Yoshinori Kamiya, Keiko Bamba, Takeshi Onishi, Keiichiro Matsuda, Tatsuro Kohno, Miyuki Kurabe, Kenta Furutani, Harue Yanagimura
The journal of pain 22 ( 6 ) 715 - 729 2021.1
Congenital Tracheal Aplasia Without Prenatal Diagnosis Masked by Maternal Obesity and Gestational Diabetes: A Case Report. Reviewed International journal
Tomohiro Yamamoto, Miyuki Kurabe, Kensuke Matsumoto, Shunya Sugai, Hiroshi Baba
A&A practice 14 ( 6 ) e01200 2020.4
Intravenous administration of lidocaine directly acts on spinal dorsal horn and produces analgesic effect: An in vivo patch-clamp analysis (vol 6, 26253, 2016) Reviewed
Miyuki Kurabe, Hidemasa Furue, Tatsuro Kohno
SCIENTIFIC REPORTS 7 46814 2017.6
Perforation of the superior vena cava 5 days after insertion of a central venous catheter through the left internal jugular vein Reviewed
Miyuki Kurabe, Tatsunori Watanabe, Tatsuro Kohno
JOURNAL OF CLINICAL ANESTHESIA 31 193 - 196 2016.6
Intravenous administration of lidocaine directly acts on spinal dorsal horn and produces analgesic effect: An in vivo patch-clamp analysis Reviewed
Miyuki Kurabe, Hidemasa Furue, Tatsuro Kohno
SCIENTIFIC REPORTS 6 26253 2016.5
トラネキサム酸の脊髄後角における抑制性シナプス伝達に対する作用
大橋 宣子, 佐々木 美佳, 山本 豪, 倉部 美起, 古谷 健太, 大橋 正幸, 紙谷 義孝, 馬場 洋, 河野 達郎
脊髄機能診断学 35 ( 1 ) 52 - 57 2015.1
痛みの薬物治療 (痛みのScience & Practice)
山本 達郎
文光堂 2013.7 （ ISBN:4830628340 ）
Intravenous administration of lidocaine inhibits excitatory synaptic transmission in spinal dorsal horn neurons
倉部 美起, 大橋 宣子, 山本 豪
脊髄機能診断学 35 ( 1 ) 46 - 51 2014
4 消化器手術後のC. diffidile腸炎の検討(II.主題,第59回新潟大腸肛門病研究会)
倉部 美起, 酒井 靖夫, 坪野 俊広, 武者 信行, 田邊 匡, 桑原 明史, 角田 和彦
新潟医学会雑誌 122 ( 7 ) 390 - 390 2008.7
Manipulating Descending Pain Suppression Systems Using Chemogenetics to Find Seeds for Chronic Pain Treatment
Grant number：22H03167
2022.4 - 2026.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (B)
Awarding organization：Japan Society for the Promotion of Science
Grant amount：\16510000 （ Direct Cost: \12700000 、 Indirect Cost：\3810000 ）
光遺伝学を利用した青斑核ニューロン制御による全身麻酔薬作用機序の解明
Grant number：21K08943
2021.4 - 2024.3
System name：科学研究費助成事業
Research category：基盤研究(C)
Awarding organization：日本学術振興会
倉部 美起, 佐々木 美佳, 上野 将紀
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
全身麻酔薬の作用機序は現代においても未解明である。睡眠・覚醒と密接な関わりを持つ、“青斑核”を起始核とするノルアドレナリン（NA）ニューロンが全身麻酔時の意識消失や覚醒にも関与していると考えられているが、詳細は不明である。近年、青斑核ニューロンが一様ではなく、NAニューロン以外にGABA（gamma-Aminobutyric acid）ニューロンを含み、相互作用していることが明らかになったことから、GABAニューロンとNAニューロンとの相互の関係性が、全身麻酔薬の作用機序に大きく関与しているのではないかと考えた。そこで、全身麻酔時の青斑核NA/GABAニューロンの活動が果たす機能的役割とそれぞれの神経の相互関連性を、光遺伝学的手法を用いて解明することを目的とした。
初年度は光遺伝学的手法の導入と確認を組織学的・生理学的両面から行った。青斑核NAニューロンを特異的に操作するために、NAニューロン特異的プロモーターPRSx8下でチャネルロドプシン・アーキロドプシンを発現するアデノ随伴ウイルスを作製し、C57BL/6マウス青斑核に脳定位固定装置を用いて投与した。免疫組織学的に、青斑核ニューロンに特異的に発現することを確認した。さらにこれらのマウスから青斑核スライス標本を作製し近赤外線
微分干渉顕微鏡を用いて可視下にホールセルパッチクランプ記録を行った。NAニューロンは電流固定モード下で３～４Hzの活動電位を発していた。また、光刺激によって活動電位の発火頻度が増加あるいは減少することを確認した。
これらの結果を基に、次年度はNA/GABAニューロン制御下に電気生理学解析を進め、無麻酔状態のマウスからの脳波・行動解析を行う予定である。
Why does neuropathic pain spread beyond the area of control of the injured peripheral nerves?
Grant number：20H03775
2020.4 - 2023.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (B)
Awarding organization：Japan Society for the Promotion of Science
Grant amount：\10660000 （ Direct Cost: \8200000 、 Indirect Cost：\2460000 ）
Elucidating mechanisms of action of general anesthetics using chemogenetics
Grant number：19K22652
2019.6 - 2021.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Challenging Research (Exploratory)
Awarding organization：Japan Society for the Promotion of Science
Kamiya Yoshinori
Grant amount：\6240000 （ Direct Cost: \4800000 、 Indirect Cost：\1440000 ）
The purpose of this study was to use chemogenetics to manipulate noradrenergic (NA) neurons in the locus coeruleus to determine the role of NA neurons in the loss of consciousness and arousal during general anesthesia. To specifically manipulate the NA neurons, an adeno-associated virus (AAV1-PRSx8-hM3Dq-HA) was prepared and injected into the rat locus coeruleus. NA neuron activation only slightly altered the induction and arousal times of volatile anesthetics, but markedly altered the induction and arousal times of intravenous anesthetics. Furthermore, we established a method for electrophysiological analysis under free moving and under the control of the NA neurons
Brain-Spinal Cord Plasticity Changes during the Establishment of Chronic Pain: Elucidation of the Role of Inhibitory Neural Circuits
Grant number：18K08811
2018.4 - 2021.3
System name：Grants-in-Aid for Scientific Research
Research category：Grant-in-Aid for Scientific Research (C)
Awarding organization：Japan Society for the Promotion of Science
Miyuki Kurabe
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
We hypothesized that not only hyperexcitability of excitatory neurotransmission but also hyperexcitability of inhibitory neuronal circuits may be involved in the synaptic plasticity changes that develop during the establishment of chronic pain. We aimed to elucidate the role of inhibitory circuits in spinal plasticity from the early stage of peripheral nerve injury to the establishment of chronic pain, focusing especially on the descending inhibitory system.
Using the DREADD system, we selectively activated neurons in locus coeruleus, one of the originating nuclei of the descending inhibitory system, and analyzed their effects on pain responses. In addition to the in vivo patch-clamp method, behavioral and immunohistological analyses were also performed to conduct an integrated study to elucidate the role of the descending inhibitory system in pain.
Physiological analysis of spinal cord-brain coupling in neuropathic pain and elucidation of glial cell activation mechanism.
Grant number：18H02897
2018.4 - 2021.3
System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category：Grant-in-Aid for Scientific Research (B)
Awarding organization：Japan Society for the Promotion of Science
Kamiya Yoshinori
Grant amount：\15730000 （ Direct Cost: \12100000 、 Indirect Cost：\3630000 ）
In this study, physiological changes in neuronal excitation in the spinal dorsal horn, the relay point of perception, and in the primary sensory cortex, the endpoint of perception, were measured longitudinally after peripheral nerve injury using flavoprotein fluorescence imaging and in vivo patch-clamp techniques. Although pain thresholds were consistently lowered after peripheral nerve injury, excitation in the cerebral cortex increased over time, while excitation in the spinal cord tended to decrease. The patch-clamp technique revealed that excitatory and inhibitory synaptic transmission in the spinal cord changes dynamically over time. The changes in synaptic transmission in the dorsal horn of the spinal cord could be suppressed by astrocyte inhibition, but astrocyte inhibition was ineffective in different chronic pain models.
Contribution of glycinnergic neurons to spinal plasticity in neuropathic pain
Grant number：16K20083
2016.4 - 2018.3
System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Research category：Grant-in-Aid for Young Scientists (B)
Awarding organization：Japan Society for the Promotion of Science
KUEABE MIYUKI
Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）
Little is known about the role of glycineergic neuron of spinal dorsal horn in neuropathic pain. We analyzed changes of inhibitory neuronal transmission between normal rats and neuropathic pain model rats using in vivo patch-clamp technique. As a result, only the amplitude of the inhibitory postsynaptic current decreased. There was no difference in the frequency and amplitude of glycineergic IPSC between the two groups. Furthermore, in model rats relative GABAergic neurons increased. The balance changes of these inhibitory neurons were thought to play a part of plasticity change in neuropathic pain.
Underlying mechanisms of intravenous administration of lidocaine in spinal dorsal horn
Grant number：25861365
2013.4 - 2015.3
System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Research category：Grant-in-Aid for Young Scientists (B)
Awarding organization：Japan Society for the Promotion of Science
KURABE Miyuki
Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）
Intravenous administration of lidocaine (IVL) is used for the management of postoperative and neuropathic pain. However, the analgesic mechanisms of IVL are yet to be elucidated. We hypothesized that IVL may affect excitatory synaptic transmission in spinal dorsal horn neurons. Herein, we used in vivo patch-clamp recording to examine the effects of IVL on synaptic transmission in the dorsal horn neurons of adult male Wistar rats. The experiments were performed in the voltage-clamp mode at a holding potential of -70 mV. IVL (3 and 10 mg/kg) decreased sEPSC frequencies, but did not affect the amplitudes. IVL (10 mg/kg) also decreased the area under the curve of EPSCs evoked by peripheral pinch stimuli to the receptive field. However IVL, superfusion of lidocaine (100 μM) on the spinal surface did not affect sEPSC frequencies or amplitudes. Our results suggest that IVL inhibits excitatory synaptic transmission in spinal dorsal horn neurons, which may be one of the analgesic mechanisms.
