Faculty of Medicine School of Health Sciences Medical Technology Professor
Updated on 2026/08/25
博士(歯学) ( 1993.3 新潟大学 )
歯学士 ( 1989.3 新潟大学 )
口腔細菌
保健学
口腔衛生
臨床化学
Life Science / Lifelong developmental nursing
Life Science / Social dentistry / 口腔衛生学
Life Science / Oral pathobiological science
Niigata University Faculty of Medicine School of Health Sciences
2026.4
Country:Japan
Niigata University Professor
2026.4
Country:Japan
Niigata University Faculty of Medicine School of Health Sciences
2024.4 - 2026.3
Country:Japan
Niigata University Faculty of Medicine School of Health Sciences
2019.2 - 2022.1
Country:Japan
Niigata University Graduate School of Health Sciences Professor
2016.4 - 2026.3
Tohoku University Graduate School of Dentistry Lecturer
2003.7 - 2016.3
Tohoku University Graduate School of Dentistry Research Assistant
2001.4 - 2003.6
Tohoku University Graduate School of Dentistry
1999.4 - 2001.3
Country:Japan
ニューヨーク州立大学バッファロー校 歯学部 (海外研修)
1998.7 - 1999.3
Country:United States
Niigata University Faculty of Dentistry School of Dentistry Assistant
1998.4 - 1999.3
State University of New York at Buffalo Postdoctoral Associate
1997.4 - 1998.3
Country:United States
Niigata University Graduate School of Dentistry
1996.4 - 1997.3
Country:Japan
ブリストル大学 歯学部 海外研修(客員研究員)
1995.10 - 1995.11
Country:United Kingdom
ブリストル大学 歯学部 海外研修(客員研究員)
1995.1 - 1995.2
Country:United Kingdom
Niigata University Faculty of Dentistry
1993.4 - 1996.3
Country:Japan
Niigata University Faculty of Medicine School of Health Sciences Medical Technology Professor
2016.4
Niigata University Faculty of Dentistry School of Dentistry Research Assistant
1998.4 - 1999.3
Niigata University Graduate School, Division of Dental Research 歯学臨床系
1989.4 - 1993.3
Country: Japan
Niigata University Faculty of Dentistry 歯学科
1983.4 - 1989.3
Country: Japan
International Association for Dental Research
歯科基礎医学会
日本臨床検査学教育学会
日本臨床化学会
日本細菌学会(中部支部会)
口腔保健用機能性食品研究会
日本口腔衛生学会
大学女性協会 賛助会員
日本臨床化学会 評議員
2025.4
Committee type:Academic society
日本臨床化学会(甲信越支部) 幹事
2022.7
Committee type:Academic society
日本臨床検査学教育学会 学術評議員
2022.5
Committee type:Academic society
大学女性協会 賛助会員
2022.4
Committee type:Academic society
口腔保健用機能性食品研究会 理事
2018.2 - 2024.12
Committee type:Academic society
日本細菌学会(中部支部会) 評議員
2017.10
Committee type:Academic society
新潟大学全学同窓会・運営委員会 委員(歯学部同窓会)
2017.4 - 2022.3
Committee type:Other
日本臨床化学会 正会員
2015.11
Committee type:Academic society
歯科基礎医学会 代議員
2004.9
Committee type:Academic society
国際歯科研究学会(IADR)および日本部会(JADR) 正会員
1993.4
Committee type:Academic society
歯科基礎医学会 正会員
1993.4
Committee type:Academic society
歯科基礎医学会 学生会員
1992.4 - 1993.3
Committee type:Academic society
国際歯科研究学会(IADR)および日本部会(JADR) 学生会員
1991.7 - 1993.3
Committee type:Academic society
米国、ニューヨーク州立大学バッファロー校(State University of New York at Buffalo) ポストドクトラルフェロー
1998.7 - 1999.3
米国、ニューヨーク州立大学バッファロー校(State University of New York at Buffalo) ポストドクトラルフェロー
1997.4 - 1998.3
英国、ブリストル大学(University of Bristol) 訪問研究者
1995.10 - 1995.11
英国、ブリストル大学(University of Bristol) 訪問研究者
1995.1 - 1995.2
Dentist
Microbiological analysis of the antibacterial effects of sprays applied to non-woven fabric masks Reviewed International journal
Abe T., Sekiguchi M., Wakui A., Maruyama S., Sano H., Kawachi M., Imai M., Kato Y., Sato H., Okabe R., Abiko Y., Washio J., Tanaka K., Takahashi N., Sato T.
Journal of Microorganism Control 31 ( 3 ) in press 2026.9
Microbiota in green tea beverages remaining in plastic bottles after direct drinking Reviewed International journal
Kawachi M., Wakui A., Imai M., Sekiguchi M., Sato H., Kato Y., Okabe R., Abe T., Takahashi N., Kaku N., Miyazawa M., Sato A., Naruse Y., Kubo N., Misumi A., Sano H., Abiko Y., Washio J., Tanaka K., Takahashi N., Sato T.
Microbiology and Immunology 70 ( 3 ) 182 - 188 2026.3
Microbial profiling of bold green tea bottled beverages: a screening experiment Reviewed International journal
Imai M., Kawachi M., Wakui A., Miyazawa M., Sekiguchi M., Kato Y., Sato H., Naruse Y., Asano N., Morohashi M., Sano H., Abiko Y., Washio J., Tanaka K., Takahashi N., Sato T.
Journal of Oral Biosciences 67 ( 4 ) Article Number 100697 (5 pages) 2025.12
Intentionally perforating the pulp chamber floor promotes M2 macrophage polarization in the dental pulp following tooth replantation in mice Reviewed International journal
Sano H., Nakakura-Ohshima K., Quispe-Salcedo A., Okada Y., Sato T., Ohshima H.
Journal of Oral Biosciences 67 ( 3 ) Article Number 100681 (10 pages) 2025.9
Profiling of the microbes on the surface of smartphone touchscreens Reviewed International journal
Takahashi N., Wakui A., Sekizawa Y., Kawachi M., Sekiguchi M., Abe T., Sato A., Miyazawa M., Imai M., Kaku N., Maruyama S., Sano H., Kakihara N., Washio J., Abiko Y., Tanaka K., Takahashi N., Sato T.
Journal of Oral Biosciences 67 ( 2 ) Article Number 100607 (4 pages) 2025.3
Molecular microbiological profiling of bottled unsweetened tea beverages: a screening experiment Reviewed International journal
Kaku N, Kawachi M, Wakui A, Miyazawa M, Imai M, Takahashi N, Sato A, Abe T, Sato H, Kato Y, Okabe R, Naruse Y, Sato N, Asano N, Morohashi M, Sano H, Washio J, Abiko Y, Tanaka K, Takahashi N, Sato T
Journal of Oral Biosciences 66 ( 4 ) 628 - 632 2024.9
Lactic acid bacteria in the human oral cavity: assessing metabolic functions relevant to oral health and disease Invited Reviewed International coauthorship International journal
Washio J, Abiko Y, Sato T, Takahashi N
Methods in Molecular Biology 2851 ( 14 ) 151 - 172 2024.9
Intracanal microbiome profiles of two apical periodontitis cases in one patient: a comparison with saliva and plaque profiles Reviewed International journal
Yamaki K, Tamahara T, Washio J, Sato T, Shimizu R, Yamada S
Clinical and Experimental Dental Research 10 e862 (10 pages) 2024.3
Early revascularization activates quiescent dental pulp stem cells following tooth replantation in mice Reviewed International journal
Hiroto Sano, Kuniko Nakakura-Ohshima, Angela Quispe-Salcedo, Yasuo Okada, Takuichi Sato, Hayato Ohshima
Regenerative Therapy 24 582 - 591 2023.12
The effect of formalin-fixed paraffin embedded process on salivary microbiota profiling Reviewed International journal
Sano H, Sato T, Kanri Y, Ono J, Okada Y
Biomedical Research 44 ( 3 ) 117 - 126 2023.6
The effect of intentionally perforating the floor of the pulp chamber on pulpal healing after tooth replantation in mice Reviewed International journal
Sano H, Nakakura-Ohshima K, Okada Y, Sato T, Ohshima H
Journal of Oral Biosciences 65 ( 1 ) 31 - 39 2023.3
Profiling of the microbiota in the remaining sports drink and orange juice in plastic bottles after direct drinking. Reviewed International coauthorship International journal
Miho Kawachi, Anna Wakui, Nagara Kaku, Nanase Takahashi, Shingo Maruyama, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Journal of oral biosciences 64 ( 4 ) 437 - 444 2022.12
Profiling of the microbiota of breast milk before and after feeding with an artificial nipple. Reviewed International journal
Hiroto Sano, Anna Wakui, Miho Kawachi, Shingo Maruyama, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Journal of oral biosciences 64 ( 4 ) 431 - 436 2022.12
Microbiota profiles on the surface of non-woven fabric masks after wearing Reviewed International journal
Shingo Maruyama, Hiroto Sano, Anna Wakui, Miho Kawachi, Nagara Kaku, Nanase Takahashi, Misato Miyazawa, Takashi Abe, Aya Sato, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Journal of Oral Biosciences 64 ( 3 ) 376 - 379 2022.9
Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis Reviewed International journal
Hiroto Sano, Anna Wakui, Miho Kawachi, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Journal of Oral Biosciences 63 ( 3 ) 292 - 297 2021.9
Profiling of Microbiota at the Mouth of Bottles and in Remaining Tea after Drinking Directly from Plastic Bottles of Tea Reviewed International journal
Anna Wakui, Hiroto Sano, Yuka Hirabuki, Miho Kawachi, Ayaka Aida, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Dentistry Journal 9 ( 6 ) 58 (7 pages) - 58 2021.6
Bacterial amounts and compositions in liquid baby formula and a baby drink consumed with an artificial nipple Invited Reviewed International journal
Anna Wakui, Hiroto Sano, Miho Kawachi, Ayaka Aida, Yuta Takenaka, Akane Yonezawa, Nana Nakahata, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Journal of Oral Biosciences 63 ( 2 ) 161 - 168 2021.6
A longitudinal study on the relationship of oral health at 4 years of age with that in adulthood Reviewed International journal
Yamada S, Sakashita R, Ogura M, Nakanishi E, Sato T
Dentistry Journal 9 ( 2 ) 7 (10 pages) 2021.2
Responses of oral-microflora-exposed dental pulp to capping with a triple antibiotic paste or calcium hydroxide cement in mouse molars Reviewed International coauthorship International journal
Angela Quispe-Salcedo, Takuichi Sato, Junko Matsuyama, Hiroko Ida-Yonemochi, Hayato Ohshima
Regenerative Therapy 15 216 - 225 2020.12
Nitrite-producing oral microbiome in adults and children. Reviewed International journal
Yuria Sato-Suzuki, Jumpei Washio, Dimas Prasetianto Wicaksono, Takuichi Sato, Satoshi Fukumoto, Nobuhiro Takahashi
Scientific reports 10 ( 1 ) 16652 (11 pages) - 16652 2020.10
Effects of dining-focused life enhancement program in welfare facilities for seniors in Japan Reviewed International coauthorship International journal
Sakashita R, Ono H, Sato T, Takami M, Kim W, Nakanishi E, Kusumoto H, Hamasaki M, Hamada M
Asian/Pacific Island Nursing Journal 5 ( 2 ) 63 - 72 2020.8
Impact of the Consistency of Food Substances on Health and Related Factors of Residents in Welfare Facilities for Seniors in Japan. Reviewed International journal
Reiko Sakashita, Takuichi Sato, Hiroshi Ono, Akiko Hamaue, Misao Hamada
Dentistry journal 8 ( 1 ) 9 - (10 pages) 2020.1
Izumi Mashima, Tohru Miyoshi-Akiyama, Junko Tomida, Ryo Kutsuna, Jumpei Washio, Nobuhiro Takahashi, Futoshi Nakazawa, Takuichi Sato, Yoshiaki Kawamura
Microbiology Resource Announcements 8 ( 38 ) e00397-19 2019.9
Profiling of microbiota in liquid baby formula consumed with an artificial nipple Reviewed International coauthorship International journal
Hiroto Sano, Anna Wakui, Miho Kawachi, Rito Kato, Sachie Moriyama, Mayumi Nishikata, Jumpei Washio, Yuki Abiko, Gen Mayanagi, Keiko Yamaki, Reiko Sakashita, Junko Tomida, Yoshiaki Kawamura, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato
Biomedical Research 40 ( 4 ) 163 - 168 2019.8
Consideration to health care and gender in Sri Lanka Reviewed
Gunarathne TGNS, Nakamura M, Aoki H, Taguchi M, Hotta K, Ishida M, Yamazaki Y, Sakai S, Sakagami M, Sato M, Sato T, Yamamoto H, Iwafuchi M
Journal of Health Sciences of Niigata University 16 ( 1 ) 1 - 10 2019.5
Exhaled acetone and isoprene in perioperative lung cancer patients under intensive oral care: Possible indicators of inflammatory responses and metabolic changes Reviewed
Naoko Tanda, Yasushi Hoshikawa, Takuichi Sato, Nobuhiro Takahashi, Takeyoshi Koseki
Biomedical Research 40 ( 1 ) 29 - 36 2019.2
Oral health promotion program for fostering self-management of the elderly living in communities Reviewed
Reiko Sakashita, Misao Hamada, Takuichi Sato, Yuki Abiko, Miho Takami
INTELLIGENT AUTOMATION AND SOFT COMPUTING 23 ( 3 ) 535 - 541 2017
Profiling subgingival microbiota of plaque biofilms in the elderly Reviewed
Yuki Abiko, Takuichi Sato, Reiko Sakashita, Junko Tomida, Yoshiaki Kawamura, Nobuhiro Takahashi
Journal of Oral Biosciences 58 ( 2 ) 62 - 65 2016.5
PCR-dipstick DNA chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets Reviewed
Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi
Biomedical Research 37 ( 1 ) 29 - 36 2016
Oral microbiota in crevices around dental implants: profiling of oral biofilm Invited
Sato T, Kawamura Y, Yamaki K, Ishida N, Tian L, Takeuchi Y, Hashimoto K, Abiko Y, Mayanagi G, Washio J, Matsuyama J, Takahashi N
Interface Oral Health Science 2014 45 - 50 2015.9
Sakashita R, Takami M, Ono H, Nishihira T, Sato T, Hamada M
Interface Oral Health Science 2014 335 - 351 2015.9
Porphyromonas bronchialis sp nov Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer Reviewed
Takuichi Sato, Junko Tomida, Takashi Naka, Nagatoshi Fujiwara, Ayako Hasegawa, Yasushi Hoshikawa, Junko Matsuyama, Naoko Ishida, Takashi Kondo, Kaori Tanaka, Nobuhiro Takahashi, Yoshiaki Kawamura
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE 237 ( 1 ) 31 - 37 2015.9
Responses of infected dental pulp to αTCP containing antimicrobials in rat molars Reviewed
Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
Archives of Histology and Cytology 73 ( 4+5 ) 165 - 175 2015.7
Microbiota profiling of bronchial fluids of elderly patients with pulmonary carcinoma Invited Reviewed
Naoko Ishida, Takuichi Sato, Yasushi Hoshikawa, Naoko Tanda, Keiichi Sasaki, Takashi Kondo, Nobuhiro Takahashi
Journal of Oral Biosciences 57 ( 2 ) 110 - 117 2015.5
Porphyromonas pogonae sp nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 1988 Reviewed
Yoshiaki Kawamura, Saki Kuwabara, Stephen A. Kania, Hisayuki Kato, Manami Hamagishi, Nagatoshi Fujiwara, Takuichi Sato, Junko Tomida, Kaori Tanaka, David A. Bemis
SYSTEMATIC AND APPLIED MICROBIOLOGY 38 ( 2 ) 104 - 109 2015.3
Oral malodorous gases and oral microbiota: From halitosis to carcinogenesis Invited Reviewed
Naoko Tanda, Yasushi Hoshikawa, Naoko Ishida, Takuichi Sato, Nobuhiro Takahashi, Ryoichi Hosokawa, Takeyoshi Koseki
Journal of Oral Biosciences 57 ( 4 ) 175 - 178 2015
Detection and identification of oral anaerobes in intraoperative bronchial fluids of patients with pulmonary carcinoma Reviewed
Ayako Hasegawa, Takuichi Sato, Yasushi Hoshikawa, Naoko Ishida, Naoko Tanda, Yoshiaki Kawamura, Takashi Kondo, Nobuhiro Takahashi
MICROBIOLOGY AND IMMUNOLOGY 58 ( 7 ) 375 - 381 2014.7
Rapid and Sensitive PCR-Dipstick DNA Chromatography for Multiplex Analysis of the Oral Microbiota Reviewed
Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Anne C. R. Tanner, Nobuhiro Takahashi
BIOMED RESEARCH INTERNATIONAL 2014 180323 2014
Quantification and identification of bacteria in acrylic resin dentures and dento-maxillary obturator-prostheses Reviewed
Yasuhisa Takeuchi, Kazuko Nakajo, Takuichi Sato, Shigeto Koyama, Keiichi Sasaki, Nobuhiro Takahashi
AMERICAN JOURNAL OF DENTISTRY 25 ( 3 ) 171 - 175 2012.6
Komori R, Sato T, Takano-Yamamoto T, Takahashi N
Journal of Oral Biosciences 55 ( 2 ) 107 - 112 2012.5
Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods Reviewed
Yasuhiro Ito, Takuichi Sato, Keiko Yamaki, Gen Mayanagi, Kazuhiro Hashimoto, Hidetoshi Shimauchi, Nobuhiro Takahashi
JOURNAL OF MICROBIOLOGY 50 ( 1 ) 58 - 62 2012.2
Rapid quantification of bacteria in infected root canals using fluorescence reagents and a membrane filter: A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment Reviewed
Takuichi Sato, Keiko Yamaki, Naoko Ishida, Megumi Shoji, Emika Sato, Yuki Abiko, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi
International Journal of Dentistry 2012 172935 2012
Cultivable anaerobic microbiota of infected root canals Reviewed
Takuichi Sato, Keiko Yamaki, Naoko Ishida, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Megumi Shoji, Emika Sato, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi
International Journal of Dentistry 2012 609689 2012
Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria Reviewed
Kazuhiro Hashimoto, Takuichi Sato, Hidetoshi Shimauchi, Nobuhiro Takahashi
AMERICAN JOURNAL OF DENTISTRY 24 ( 5 ) 295 - 299 2011.10
Detection and identification of non-Candida albicans species in human oral lichen planus Reviewed
Mika Masaki, Takuichi Sato, Yumiko Sugawara, Takashi Sasano, Nobuhiro Takahashi
MICROBIOLOGY AND IMMUNOLOGY 55 ( 1 ) 66 - 70 2011.1
口腔内細菌叢のコントロール Invited
河村 好章, 長谷川 義明, 佐藤 拓一
ファルマシア 46 ( 10 ) 929 - 933 2010.10
Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR Reviewed
Abiko Y, Sato T, Mayanagi G, Takahashi N
Journal of Periodontal Research 45 ( 3 ) 389 - 395 2010.6
Host beta-globin gene fragments in crevicular fluid as a biomarker in periodontal health and disease Reviewed
B. Thaweboon, P. Laohapand, C. Amornchat, J. Matsuyama, T. Sato, P. P. Nunez, H. Uematsu, E. Hoshino
JOURNAL OF PERIODONTAL RESEARCH 45 ( 1 ) 38 - 44 2010.2
Nested PCR for the sensitive detection of cariogenic bacteria Invited
Sato T, Matsuyama J, Mayanagi G, Abiko Y, Kato K, Takahashi N
Cariology Today 3-4 ( 1 ) 17 - 20 2007.3
Profiling of bacterial flora in crevices around titanium orthodontic anchor plates Reviewed
Sato R, Sato T, Takahashi I, Sugawara J, Takahashi N
Clinical Oral Implants Research 18 ( 1 ) 21 - 6 2007.2
Profiling of subgingival plaque biofilm microflora of healthy and periodontitis subjects by real-time PCR Invited
Abiko Y, Sato T, Mayanagi G, Takahashi N
Interface Oral Health Science 2007 213 - 218 2007
Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats Reviewed
Hidetoshi Mitani, Ichiro Takahashi, Kazuyuki Onodera, Jin-Wan Bae, Takuichi Sato, Nobuhiro Takahashi, Yasuyuki Sasano, Kaoru Igarashi, Hideo Mitani
HISTOCHEMISTRY AND CELL BIOLOGY 126 ( 3 ) 371 - 380 2006.9
Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction Reviewed
M Yamaura, T Sato, S Echigo, N Takahashi
ORAL MICROBIOLOGY AND IMMUNOLOGY 20 ( 6 ) 333 - 338 2005.12
Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour Reviewed
J Washio, T Sato, T Koseki, N Takahashi
JOURNAL OF MEDICAL MICROBIOLOGY 54 ( 9 ) 889 - 895 2005.9
PCR for detection of mutans streptococci in human dental plaque Invited Reviewed
Matsuyama J, Sato T, Washio J, Mayanagi G, Ito Y, Abiko Y, Hashimoto K, Miyasawa-Hori H, Nakajo K, Kato K, Takahashi N
International Congress Series 1284 158 - 162 2005
Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects
G Mayanagi, T Sato, H Shimauchi, N Takahashi
ORAL MICROBIOLOGY AND IMMUNOLOGY 19 ( 6 ) 379 - 385 2004.12
PTC gene mutation and expression of SHH, PTC, SMO, and GLI-1 in odontogenic keratocysts Reviewed International journal
Ohki K, Kumamoto H, Ichinohasama R, Sato T, Takahashi N, Ooya K
International Journal of Oral and Maxillofacial Surgery 33 ( 6 ) 584 - 592 2004.9
A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo Reviewed
K Kato, T Sato, N Takahashi, K Fukui, K Yamamoto, H Nakagaki
CARIES RESEARCH 38 ( 5 ) 448 - 453 2004
Fermentation of five sucrose isomers by human dental plaque bacteria Reviewed
Matsuyama J, Sato T, Hoshino E, Noda T, Takahashi N
Caries Research 37 ( 6 ) 410 - 415 2003.10
Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes Reviewed
T Sato, JP Hu, K Ohki, M Yamaura, J Washio, J Matsuyama, N Takahashi
ORAL MICROBIOLOGY AND IMMUNOLOGY 18 ( 5 ) 323 - 326 2003.10
Nested PCR for detection of mutans streptococci in dental plaque Reviewed
T Sato, J Matsuyama, T Kumagai, G Mayanagi, M Yamaura, J Washio, N Takahashi
LETTERS IN APPLIED MICROBIOLOGY 37 ( 1 ) 66 - 69 2003
Rapid identification of cariogenic bacteria by 16S rRNA genes PCR-RFLP analysis Invited
Sato T, Hu JP, Matsuyama J, Takahashi N
Cariology Today 2 ( 1 ) 8 - 13 2002.9
Dipeptide utilization by the periodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucleatum Reviewed
N Takahashi, T Sato
ORAL MICROBIOLOGY AND IMMUNOLOGY 17 ( 1 ) 50 - 54 2002.2
Preferential utilization of dipeptides by Porphyromonas gingivalis Reviewed
N Takahashi, T Sato
JOURNAL OF DENTAL RESEARCH 80 ( 5 ) 1425 - 1429 2001.5
Polymerase chain reaction for the detection of flaA-1 genes of oral spirochaetes in human advanced periodontal pockets Reviewed
T Sato, HK Kuramitsu
ARCHIVES OF ORAL BIOLOGY 45 ( 10 ) 921 - 925 2000.10
Metabolic pathways for cytotoxic end product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis Reviewed
N Takahashi, T Sato, T Yamada
JOURNAL OF BACTERIOLOGY 182 ( 17 ) 4704 - 4710 2000.9
16S rRNA genes PCR-RFLP analysis for rapid identification of oral anaerobic gram-positive bacilli Reviewed
Sato T, Matsuyama J, Takahashi N
International Journal of Oral Biology 25 ( 3 ) 87 - 91 2000.9
Comparison between 16S rRNA genes PCR-RFLP analysis and biochemical tests for identification of Actinomyces naeslundii Reviewed
Matsuyama J, Sato T, Takahashi N
International Journal of Oral Biology 25 ( 3 ) 83 - 86 2000.9
Restriction fragment-length polymorphism analysis of 16S ribosomal RNA genes amplified by polymerase chain reaction for rapid identification of cultivable oral treponemes Reviewed
T Sato, HK Kuramitsu
ORAL MICROBIOLOGY AND IMMUNOLOGY 14 ( 2 ) 117 - 121 1999.4
Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism Reviewed
T Sato, J Matsuyama, N Takahashi, M Sato, J Johnson, C Schachtele, E Hoshino
ARCHIVES OF ORAL BIOLOGY 43 ( 3 ) 247 - 252 1998.3
Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA Reviewed
Marchesi, JR, T Sato, AJ Weightman, TA Martin, JC Fry, SJ Hiom, WG Wade
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 64 ( 2 ) 795 - 799 1998.2
Restriction fragment-length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction Reviewed
T Sato, M Sato, J Matsuyama, S Kalfas, G Sundqvist, E Hoshino
ORAL MICROBIOLOGY AND IMMUNOLOGY 13 ( 1 ) 23 - 29 1998.2
Differentiation of Veillonella atypica, Veillonella dispar and Veillonella parvula using restricted fragment-length polymorphism analysis of 16S rDNA amplified by polymerase chain reaction Reviewed
T Sato, J Matsuyama, M Sato, E Hoshino
ORAL MICROBIOLOGY AND IMMUNOLOGY 12 ( 6 ) 350 - 353 1997.12
PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp. Reviewed
T Sato, M Sato, J Matsuyama, E Hoshino
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY 47 ( 4 ) 1268 - 1270 1997.10
Matsuyama J, Sato T, Hoshino E
Japanese Journal of Oral Biology (Journal of Oral Biosciences) 39 ( 2 ) 91 - 99 1997.4
Eubacterium exiguum sp. nov., isolated from human oral lesions Reviewed
Sergio E. Poco Jr., Futoshi Nakazawa, Tetsuro Ikeda, Michiko Sato, Takuichi Sato, Etsuro Hoshino
International Journal of Systematic Bacteriology 46 ( 4 ) 1120 - 1124 1996
Eubacterium saphenum isolated from human periodontal pockets Invited
Sato T, Uematsu H, Sato M, Nakazawa F, Hoshino E
Medical and Dental Aspects of Anaerobes 33 - 37 1995.1
IN-VITRO ANTIMICROBIAL SUSCEPTIBILITY TO COMBINATIONS OF DRUGS OF BACTERIA FROM CARIOUS AND ENDODONTIC LESIONS OF HUMAN DECIDUOUS TEETH Reviewed
T SATO, E HOSHINO, H UEMATSU, T NODA
ORAL MICROBIOLOGY AND IMMUNOLOGY 8 ( 3 ) 172 - 176 1993.6
Predominant obligate anaerobes in necrotic pulps of human deciduous teeth Reviewed
T. Sato, E. Hoshino, H. Uematsu, T. Noda
Microbial Ecology in Health and Disease 6 ( 6 ) 269 - 275 1993
口腔Eubacterium Invited
星野 悦郎, 佐藤 尚美, 中澤 太, 上松 弘幸, 佐藤 ミチ子, 池田 哲郎, 栗原 直美, 佐藤 拓一, 佐藤 牧子
新潟歯学会雑誌 22 ( 1 ) 1 - 14 1992.6
Bactericidal efficacy of a mixture of ciprofloxacin, metronidazole, minocycline and rifampicin against bacteria of carious and endodontic lesions of human deciduous teeth in vitro Reviewed
T. Sato, E. Hoshino, H. Uematsu, K. Kota, M. Iwaku, T. Noda
Microbial Ecology in Health and Disease 5 ( 4 ) 171 - 177 1992
歯科衛生学用語辞典
高橋 信博, 鷲尾 純平, 真柳 弦, 安彦 友希, 佐藤 拓一, 山本 秀輝, 佐野 拓人, 涌井 杏奈( Role: Contributor)
永末書店 2019.7 ( ISBN:9784816013683 )
高橋 信博, 佐藤 拓一, 鷲尾 純平, 中條, 和子 他( Role: Joint translator)
医歯薬出版 2012.12 ( ISBN:9784263444023 )
花田 信弘, 今井 奨, 高橋 信博, 佐藤 拓一, 鷲尾 純平( Role: Contributor)
口腔保健協会 2003.6 ( ISBN:4896051912 )
口をつけて飲んだペットボトル飲料:再飲用の可否は? Invited
涌井杏奈, 佐藤拓一
「つながる研究」2026年版(新潟大学社会連携推進機構) 2026.3
スマホ画面から検出される細菌のプロファイリング:市販UV照射器の有効性 Invited
加藤優希, 佐藤拓一
「つながる研究」2026年版(新潟大学社会連携推進機構) 2026.3
口唇微生物バイオフィルムの分子生物学的解析
今井真奈美, 佐藤拓一
「つながる研究」2026年版(新潟大学社会連携推進機構) 2026.3
祝辞(第76回支部奨学賞授与式) Invited
佐藤拓一
大学女性協会新潟支部会報 16 4 2026.3
スマートフォン画面やエレベータボタン等からの微生物の検出・解析
涌井杏奈, 佐藤拓一
「つながる研究」2025年度版(新潟大学社会連携推進機構) 2025.4
夏場、口をつけて飲んだペットボトル飲料は危険?~再飲用の可能性を探る!~
河内美帆, 佐藤拓一
「つながる研究」2025年度版(新潟大学社会連携推進機構 2025.4
卒業後の進路を選択したきっかけ・これからの決意表明 Invited
阿部峰士 (指導担当, 佐藤拓一)
新大広報 231 ( (卒業記念号) ) p. 10, 2025.3
教員からのメッセージ(医学部保健学科) Invited
佐藤拓一
大学案内 2025(新潟大学) p. 38, 2024.5
口を付けて飲んだペットボトル飲料内での口腔細菌の増殖について Invited
河内美帆, 佐藤拓一
「つながる研究」2024年度版(新潟大学社会連携推進機構) 49 2024.4
哺乳瓶による授乳で口腔細菌が液体ミルクや搾乳母乳内へ流入する! Invited
涌井杏奈, 佐藤拓一
「つながる研究」2024年度版(新潟大学社会連携推進機構) 47 2024.4
スマホ画面や使用済み不織布マスクの微生物について
涌井杏奈, 佐藤拓一
「つながる研究」2024年度版(新潟大学社会連携推進機構) 48 2024.4
「タスク・シフト/シェア」について(教育現場から見て感じること) Invited
佐藤拓一
南山会会報「轍迹」 ( 39特別号 ) 7 2024.3
卒業後の進路を選択したきっかけ・これからの決意表明 Invited
高橋七瀬 (指導担当, 佐藤拓一)
新大広報 ( 229(卒業記念号) ) p. 8, 2024.3
お祝いのことば(第74回支部奨学賞授与式) Invited
佐藤拓一
大学女性協会新潟支部会報 12 4 2024.3
大学の研究室で身近な細菌について一緒に調べてみよう!(不織布マスクやスマホ画面の細菌に関する実験) Invited
佐藤拓一
高校生アカデミック・インターンシップ研修報告書(新潟県教育委員会) 33 - 45 2024.3
搾乳母乳と新生児口腔細菌叢との連関:網羅的・分子生物学的アプローチ Invited
涌井杏奈, 佐野拓人, 河内美帆, 佐藤拓一
臨床化学 53 ( 1 ) 44 - 46 2024.1
ワンランク上の臨床検査技師を目指して Invited
今井真奈美 (指導担当, 佐藤拓一)
保健学科案内パンフレット(2024年度版) 新潟大学 10 2023.7
私の研究事例(Study Case)03,医学部保健学科 検査技術科学プログラム Invited
今井真奈美 (指導担当, 佐藤拓一)
大学案内 2024(新潟大学) 9 - 10 2023.4
在校生からのメッセージ,医学部保健学科 Invited
宮沢美里 (指導担当, 佐藤拓一)
大学案内 2024(新潟大学) 54 2023.4
手指の常在菌叢:エタノール消毒および手洗いの効果 Invited
高橋七瀬, 佐藤拓一
「つながる研究紹介」2022年度版(新潟大学地域創生推進機構) 51 2023.4
使用済みの不織布マスクに付着・生息する常在菌叢の解析 Invited
丸山伸吾, 佐藤拓一
「つながる研究紹介」2022年度版(新潟大学地域創生推進機構) 50 2023.4
哺乳瓶ニプルを介して口腔細菌が液体ミルクや搾乳母乳内へ流入する! Invited
涌井杏奈, 佐藤拓一
「つながる研究紹介」2022年度版(新潟大学地域創生推進機構) 48 2023.4
ペットボトル飲料に口を付けて飲むと口腔細菌が流入する! Invited
河内美帆, 佐藤拓一
「つながる研究紹介」2022年版(新潟大学地域創生推進機構) 49 2023.4
《新潟大学》検査技術科学専攻1~3年生の近況 Invited
佐藤拓一
後援会だより《新潟大学医学部保健学科後援会》 ( 35 ) 8 2023.3
《新潟大学》令和4年度 検査技術科学専攻4年生の近況 Invited
佐藤拓一, 山本秀輝
後援会だより《新潟大学医学部保健学科後援会》 ( 35 ) 5 - 6 2023.3
コロナ禍の検査技術科学専攻を、保健学科HP掲載の記事をもとに振り返る Invited
佐藤拓一
南山会会報「轍迹」 ( 38 ) 5 2023.3
ワンランク上の臨床検査技師を目指して Invited
宮沢美里 (指導担当, 佐藤拓一)
保健学科案内パンフレット(2023年度版) 新潟大学 10 2022.7
新潟大学・大学院保健学研究科・臨床化学研究室の紹介,甲信越支部「支部研究室だより」 Invited
佐藤拓一
臨床化学 51 ( 3 ) 263 - 264 2022.7
使用済みマスクに付着・生息する皮膚常在菌叢プロファイリング Invited
丸山伸吾, 佐藤拓一
「つながる研究紹介」2021年度版(新潟大学地域創生推進機構) 45 2022.3
ペットボトル飲料をラッパ飲みすると口腔細菌が流入する! Invited
河内美帆, 佐藤拓一
つながる研究紹介」2021年度版(新潟大学地域創生推進機構) 44 2022.3
手洗い前後の手指の皮膚常在菌叢プロファイリング Invited
高橋七瀬, 佐藤拓一
「つながる研究紹介」2021年度版(新潟大学地域創生推進機構) 46 2022.3
クラス会報告;今年(2022年7月に)19期生クラス会を開催します! Invited
佐藤拓一
新潟大学歯学部同窓会誌 ( 42 ) 83 - 84 2022.3
哺乳瓶ニプルを介して口腔細菌が液体ミルク内へ流入する! Invited
涌井杏奈, 佐藤拓一
「つながる研究紹介」2021年度版(新潟大学地域創生推進機構) 43 2022.3
新潟大学大学院保健学研究科の活動報告=特別シリーズ=科学技術振興機構『さくらサイエンスプラン』友情と感激 Invited
佐藤 拓一
週刊『文教ニュース』 ( 2613 ) 46 - 47 2020.8
哺乳瓶乳首を介して乳児用飲料内へ流入する口腔細菌 Invited
涌井 杏奈, 佐藤 拓一
「つながる研究紹介」2020年版(新潟大学地域創生推進機構) 2020.8
Sato T
JST Sakura Science Plan, Activity Report of Open Application Course 25 2020.6
【巻頭言】ペットボトル飲料をラッパ飲みすると・・・ Invited
佐藤 拓一
高校への数学 45 ( 7 ) 1 - 1 2020.6
《活動レポート》スリランカの若手大学教員を招き保健学最先端研究の体験型演習を実施し、日本とスリランカの将来構想を共に考えた Invited
佐藤 拓一
日本・アジア青少年サイエンス交流事業(さくらサイエンスプラン)2019年度活動レポート(一般公募コース)(国立研究開発法人・科学技術振興機構) ( 273 ) 2020.3
《新潟大学》保健学科および保健学研究科における国際交流事業について【2019年の国際交流活動】 Invited
佐藤 拓一
後援会だより《新潟大学医学部保健学科後援会》 ( 33 ) 4 - 5 2020.1
《研究報告書》液体ミルクの安全性の立証に向けて:飲み残したペットボトルの口の部分に付着する細菌および飲料物内の細菌の量および構成の解析 Invited
佐藤 拓一, 鷲尾 純平
総合健康推進財団 第34回 一般研究奨励助成事業による研究報告書 49 - 56 2019.8
《新潟大学》全学同窓会交流会→次回は歯学部同窓会が担当!!! @2019年10月19日(土) Invited
佐藤 拓一
新潟大学歯学部同窓会誌 ( 39 ) 57 - 58 2019.3
液体ミルクの安全性の立証に向けて:飲料物の飲み口に付着する細菌の量および構成の解析 Invited Reviewed
佐野拓人, 佐藤拓一, 鷲尾純平
2017年度やずや食と健康助成研究【チャレンジ部門】研究成果報告書 (6ページ) 2019.1
中澤太教授の御退任に寄せて Invited
佐藤 拓一
中澤太教授退任記念誌 20 2018.3
教授就任の抱負、ご挨拶《新潟大学・大学院保健学研究科》 Invited
佐藤 拓一
新潟大学歯学部同窓会誌 ( 37 ) 53 - 54 2017.3
《JADR》理事会、評議員会および総会報告 Invited
佐藤 拓一
Newsletter for JADR 2015 ( 1 ) 13 - 15 2015.3
《歯学情報(受賞報告)》Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 5th International Symposium for Interface Oral Health Science【The Excellent Poster Award】 A rapid and simple detection of plaque bacteria using a novel molecular metho Invited
Tian L, Sato T
東北大学歯学雑誌 33 ( 1 ) 38 2014.6
《JADR》理事会、評議員会および総会報告 Invited
佐藤 拓一
Newsletter for JADR 2014 ( 1 ) 7 - 8 2014.2
蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法 Invited
庄司 恵, 佐藤 愛美加, 佐藤 拓一, 石田 直子, 八巻 惠子, 高橋 信博
歯界展望 ( 特別号 ) 294 2013
口腔バイオフィルムの分子生物学的プロファイリング-メタゲノム解析- Invited
佐藤 拓一, 河村 好章, 八巻 惠子, 島内 英俊, 高橋 信博
歯界展望 ( 特別号 ) 190 2013
A method for quantitatively evaluating plaque biofilm removing capacity of a dental water jet using EPMA
Kato K, Tamura K, Nakagaki H, Sakakibara S, Ou Y, Matsumoto S, Fujita K, Sato T
Interface Oral Health Science 2011 186 - 188 2012.2
Subgingival plaque biofilm microflora of elderly subjects: quantitative analysis of Porphyromonas gingivalis and genotyping of its virulence-associated fimA.
Abiko Y, Sato T, Sakashita R, Takahashi N
Interface Oral Health Science 2011 176 - 177 2012.2
Community oral health promotion program fostering self-management for elderly people
Nishihira T, Nishitani M, Sato T, Abiko Y, Matsushita K, Hamada M, Sakashita R
Interface Oral Health Science 2011 317 - 319 2012.2
Quantification and identification of bacteria in the maxillary obturator-prostheses
Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
Interface Oral Health Science 2011 209 - 211 2012.2
Change in infected root canal microflora during the course of root canal therapy
Yamaki K, Sato T, Hasegawa A, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
Interface Oral Health Science 2011 221 - 222 2012.2
Silent aspiration of oral bacteria – Microbiological analysis of intraoperative bronchial fluids from patients with pulmonary carcinoma
Hasegawa A, Sato T, Hoshikawa Y, Kondo T, Takahashi N
Interface Oral Health Science 2011 181 - 182 2012.2
編集後記《Newsletter@東北大学大学院歯学研究科》 Invited
佐藤 拓一
Newsletter 東北大学大学院歯学研究科 ( 8 ) 4 2012.1
Involvement of cough reflex impairment and silent aspiration of oral bacteria in postoperative pneumonia: a model of aspiration pneumonia
Takuichi Sato, Yasushi Hoshikawa, Takashi Kondo, Kazuhiro Hashimoto, Yuki Abiko, Ayako Hasegawa, Junko Matsuyama, Nobuhiro Takahashi
INTERFACE ORAL HEALTH SCIENCE 2009 273 - + 2010
Can symptom awareness of the elderly be a clue to find oral diseases and promote oral health behaviors?
Reiko Sakashita, Tomoko Miyashiba, Kumiko Otsuka, Takuichi Sato, Michiko Kamide, Kayo Watanabe, Naomi Takimoto, Mariko Kawaguchi, Tomoko Nishihira
INTERFACE ORAL HEALTH SCIENCE 2009 346 - + 2010
Profiling of dental plaque microflora on root caries lesions and the protein-degrading activity of these bacteria
Kazuhiro Hashimoto, Takuichi Sato, Hidetoshi Shimauchi, Nobuhiro Takahashi
INTERFACE ORAL HEALTH SCIENCE 2009 243 - + 2010
Profiling of dented plaque biofilm on first molars with orthodontic bands and brackets
Ryo Komori, Takuichi Sato, Teruko Takano-Yamamoto, Nobuhiro Takahashi
INTERFACE ORAL HEALTH SCIENCE 2009 248 - + 2010
Porphyromonas gingivalis is widely distributed in subgingival plaque biofilm of elderly subjects
Yuki Abiko, Takuichi Sato, Kenji Matsushita, Reiko Sakashita, Nobuhiro Takahashi
INTERFACE ORAL HEALTH SCIENCE 2009 240 - + 2010
《新潟大学歯学部》 口腔環境・感染防御学分野 星野悦郎教授の御定年退職に寄せて Invited
佐藤 拓一
歯学部ニュース《新潟大学》 ( 118 ) 33 - 34 2010
Detection of viable bacterial cells in acrylic resin denture bases
Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Sasaki K, Takahashi N
Interface Oral Health Science 2009 230 - 231 2010
Real-time PCR analysis of cariogenic bacteria in supragingival plaque biofilm microflora on caries lesions of children Invited
Junko Matsuyama, Takuichi Sato, Yuki Abiko, Ayako Hasegawa, Kazuo Kato, Etsuro Hoshino
INTERFACE ORAL HEALTH SCIENCE 2009 271 - + 2010
A method for determining the profiles of biomass volume and glucan within dental plaque Invited
Kazuo Kato, Kiyomi Tamura, Tran Thu Thuy, Haruo Nakagaki, Takuichi Sato
INTERFACE ORAL HEALTH SCIENCE 2009 237 - + 2010
Real-time PCR analysis of genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of children Invited
Matsuyama J, Sato T, Takahashi N, Sato M, Hoshino E
Interface Oral Health Science 2007 255 - 256 2008.4
Detection of periodontopathic bacteria in periodontal pockets by nested polymerase chain reaction Invited
Sato T, Abiko Y, Mayanagi G, Matsuyama J, Takahashi N
Interface Oral Health Science 2007 267 - 268 2008.4
Influence of yogurt products containing Lactobacillus reuteri on distributions of mutans streptococci within dental plaque Invited
Kato K, Tamura K, Sato T, Nakagaki H
Interface Oral Health Science 2007 259 - 260 2008.4
今の時代に思うこと 《新潟大学歯学部・野田忠教授御退官に寄せて》 Invited
佐藤 拓一
野田忠教授退官記念誌 20 - 21 2007.3
Candida species as members of oral microflora in oral lichen planus
Masaki M, Sato T, Sugawara Y, Sasano T, Takahashi N
Interface Oral Health Science 2007 273 - 274 2007
Microflora profiling of root canal utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes -Differences between before and after root canal treatments-
Ito Y, Sato T, Mayanagi G, Yamaki K, Shimauchi H, Takahashi N
Interface Oral Health Science 2007 265 - 266 2007
Quantication and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction. (vol 20, pg 333, 2005)
M Yamaura, T Sato, S Echigo, N Takahashi
ORAL MICROBIOLOGY AND IMMUNOLOGY 21 ( 2 ) 136 - 136 2006.4
《平成17年度新潟大学歯学部同窓会・総会学術講演》「象牙質・歯髄複合体研究の進展と再生医療への生物学的基盤」大島勇人教授の講演を拝聴して Invited
佐藤 拓一
新潟大学歯学部同窓会誌 ( 26 ) 14 2006.3
《平成17年度新潟大学歯学部同窓会・総会学術講演》「象牙質・歯髄複合体研究の進展と再生医療への生物学的基盤」大島勇人教授の講演を拝聴して Invited
佐藤 拓一
歯学部ニュース《新潟大学》 ( 107 ) 57 2005.10
Density profiles of total bacteria and S. mutans within dental plaque treated with stannous fluoride gel Invited Reviewed
K. Kato, K. Fukui, H. Nakagaki, T. Sato, N. Takahashi
International Congress Series 1284 185 - 186 2005.9
Microflora profiling of subgingival and supragingival plaque of healthy and periodontitis subjects by nested PCR Reviewed
G. Mayanagi, T. Sato, H. Shimauchi, N. Takahashi
International Congress Series 1284 195 - 196 2005.9
Relationship between hydrogen sulfide-producing bacteria of the tongue coating and oral malodor Invited Reviewed
J. Washio, T. Sato, K. Ikawa, N. Tanda, M. Iwakura, T. Koseki, N. Takahashi
International Congress Series 1284 199 - 200 2005.9
Quantification of total bacterial DNA by real-time PCR and detection of bacteria by nested PCR from postoperative maxillary cyst Reviewed
M. Yamaura, T. Sato, S. Echigo, N. Takahashi
International Congress Series 1284 193 - 194 2005.9
Microflora profiling of crevices around titanium orthodontic anchor plates Reviewed
R. Sato, T. Sato, I. Takahashi, J. Sugawara, N. Takahashi
International Congress Series 1284 197 - 198 2005.9
高齢者歯髄の免疫防御機構に関する研究
大島 勇人, 佐藤 拓一, 高橋 信博, 野村 修一, 大島 邦子, 監物 新一, 川岸 恵理子, 楯 泰昌
大和証券ヘルス財団研究業績集 ( 27 ) 98 - 103 2004.3
抗菌性薬剤に対するラット臼歯感染歯髄の反応
大島 勇人, 佐藤 拓一, 監物 新一, 高橋 信博
歯科基礎医学会雑誌 45 ( 5 ) 287 - 287 2003.9
Combination of genus-specific PCR and RFLP analysis for identification of oral streptococci.
T. Sato, M. Yamaura, J. Matsuyama, N. Takahashi
JOURNAL OF DENTAL RESEARCH 82 B80 - B80 2003.6
Detection of 25 periodontopathic bacteria from periodontal sites by nested PCR.
G. Mayanagi, T. Sato, H. Shimauchi, N. Takahashi
JOURNAL OF DENTAL RESEARCH 82 B153 - B153 2003.6
口腔領域の慢性感染症、特に下顎骨骨髄炎及び感染性歯根嚢砲に関する研究 Invited
佐藤 拓一, 高橋 信博, 山浦 みゆき, 越後 成志, 鷲尾 純平, 長坂 浩, 松山 順子, 大島 勇人
大和証券ヘルス財団の助成による研究業績集 26 10 - 14 2003.3
Microbiological comparison between root caries lesion and proximal dental plaque.
T Wakui, M Nakano, M Yajima, Y Momoi, T Sato, N Takahashi, N Maeda
JOURNAL OF DENTAL RESEARCH 81 A351 - A351 2002.3
Rapid detection of periodontal pathogens by multiplex PCR.
T Sato, J Matsuyama, H Miyasawa, N Takahashi
JOURNAL OF DENTAL RESEARCH 79 396 - 396 2000
Metabolic pathways for cytotoxic end-products formation by Porphyromonas gingivalis.
N Takahashi, T Sato, T Yamada
JOURNAL OF DENTAL RESEARCH 79 394 - 394 2000
東南アジアにおける歯周疾患に関する国際共同研究-特に日本、バングラデシュ、フィリピンの比較実態調査と文化的背景分析- Invited
星野 悦郎, 佐藤 拓一, M.A. Kalam Joarde
Pfizer Health Research News 10 21 - 24 1995.12
混合抗菌剤を添加した光硬化型グラスアイオノマーセメントの抗菌性について Invited
佐藤 拓一
新歯界 ( 527 ) 28 1995.12
《最近のトピックス》最近の細菌分類について Invited
中澤 太, 上松 弘幸, 佐藤 拓一, 星野 悦郎
新潟歯学会雑誌 25 ( 1 ) 61 - 62 1995.6
パ-シャルデンチャ-の装着と口腔病変 Invited
星野 悦郎, 佐藤 拓一, 斎藤)律子
日本歯科評論 ( 別冊「パ-シャルデンチャ-をみる目」 ) 67 - 77 1995.4
口腔の細菌学的環境 Invited
星野 悦郎, 佐藤 拓一, 斎藤)律子
日本歯科評論 ( 別冊「パ-シャルデンチャ-をみる目」 ) 59 - 66 1995.4
《海外レポート》 英国・ブリストル大学、3ケ月滞在記 Invited
佐藤 拓一
歯学部ニュース《新潟大学》 ( 83 ) 1995
『日本学術振興会特別研究員』をご存知ですか?
佐藤 拓一
歯学部ニュース《新潟大学》 ( 82 ) 1994
「歯学部ニュース」が廃刊って本当ですか?
佐藤 拓一
歯学部ニュース《新潟大学》 ( 82 ) 1994
《最近のトピックス》歯周ポケット内細菌叢の栄養学的特徴 Invited
上松 弘幸, 佐藤 拓一, 星野 悦郎
新潟歯学会雑誌 22 ( 2 ) 145 - 146 1992.12
Microbial screening in oolong tea beverages remaining in plastic bottles International conference
Kawachi M., Sato H., Wakui A., Sekiguchi M., Kato Y., Naruse Y., Kubo N., Misumi A., Abiko Y., Washio J., Sato T.
The 104th Annual Meeting of the IADR (San Diego, USA) 2026.3
Profiling of microbiota in the remaining bottled black-tea and coffee-beverages International conference
Sato H., Kawachi M., Wakui A., Sekiguchi M., Kato Y., Naruse Y., Abiko Y., Washio J., Sato T.
The 104th Annual Meeting of the IADR (San Diego, USA) 2026.3
スマートフォン/タブレット画面に付着・生息する細菌:手指との比較
五十嵐優翔, 伊藤菜々, 小田直太郎, 加藤優希, 涌井杏奈, 佐藤拓一
第99回日本細菌学会総会(広島)「中・高校生研究発表セッション」 2026.3
使用済みマスクに生息する細菌の分析~細菌の量からマスクの再利用を考える~
小田直太郎,丸山伸吾,阿部峰士,高橋七瀬,佐藤拓一
ジュニアドクター育成塾 サイエンスカンファレンス2022(オンライン) 2022.11
飲みかけのペットボトル飲料(お茶、スポーツ飲料、コーラ、オレンジジュース)に生息する細菌について
畑 歩花, 本間沙也花, 河内美帆, 関沢有芽, 佐藤拓一
ジュニアドクター育成塾 サイエンスカンファレンス2020(録画発表) 2020.11
An history of relationship between Faculty of Allied Health Sciences, University of Peradeniya and Niigata University School of Health Sciences Invited
Sato T
The 4th GSH International Symposium, “PAST and Future of International Exchange” 2019.12
Profiling of microbiota on smartphone touchscreens and elevator buttons: Comparison with finger skin International conference
Wakui A., Kato Y., Kawachi M., Imai M., Abe T., Sato H., Kubo N., Misumi A., Akagawa T., Washio J., Sato T.
IUMS 2026 (Lisbon, Portugal) 2026.11
Molecular biological profiling of microbial biofilms on the surface of the human lips International conference
Imai M., Okabe R., Wakui A., Kawachi M., Kato Y., Sato H., Kubo N., Misumi A., Akagawa T., Morohashi M., Asano N., Washio J., Sato T.
IUMS 2026 (Lisbon, Portugal) 2026.11
Microbiota profiling of the remaining bottled oolong tea beverages: a screening experiment International conference
Kawachi M., Sato H., Wakui A., Kato Y., Kubo N., Misumi A., Akagawa T., Imai M., Washio J., Sato T.
IUMS 2026 (Lisbon, Portugal) 2026.11
Detection and identification of putative oral bacteria on smartphone touchscreens International conference
Wakui A., Kato Y., Sato H., Kubo N., Misumi A., Kawachi M., Imai M., Abe T., Abiko Y., Washio J., Takahashi N., Sato T.
International Oral Health Symposium 2026 (Tokyo) 2026.10
Profiling of microbiota isolated from smartphone touchscreens: Detection of oral bacteria and effectiveness of UV irradiation International conference
Kato Y., Wakui A., Sato H., Misumi A., Kubo N., Abe T., Kawachi M., Imai M., Akagawa T., Morohashi M., Asano N., Washio J., Sato T.
The 64th Annual Scientific Meeting of the IADR ANZ (Perth, Australia) 2026.10
Molecular microbiological profiling in the remaining bottled black tea and coffee beverages International conference
Sato H., Kawachi M., Wakui A., Kato Y., Kubo N., Misumi A., Imai M., Okabe R., Akagawa T., Morohashi M., Asano N., Washio J., Sato T.
The 64th Annual Scientific Meeting of the IADR ANZ (Perth, Australia) 2026.10
Detection and identification of oral bacteria on smartphone touchscreens
Wakui A., Kato Y., Kawachi M., Sano H., Abiko Y., Washio J., Takahashi N., Sato T.
The Update Symposium in the 68th Annual Meeting of the JAOB (Nagoya) 2026.9
ワンランク上を目指す研究室:佐藤拓一研究室のとりくみ Invited
佐藤拓一, 涌井杏奈, 河内美帆
大学女性協会新潟支部2026年度総会(新潟) 2026.4
博士前期課程および学部学生による、海外で現地開催される国際学会発表への挑戦:(その3)欧米における、本格的な学会での発表体験 Invited
佐藤拓一
令和7年度 新潟大学全学FD・SD 主体的学修を推進する新潟大学の取り組み(学長教育助成制度成果報告会) 2026.3
保健学研究科におけるグローバル対応力養成教育の現状と今後 Invited
佐藤拓一
第9回グローバル推進機構FD・SD「大学院におけるグローバル対応力養成教育の現状と今後~国際共同学位プログラムの拡充に向けて~」 2025.12
夏季長期休暇を活用した、小中学生向けの科学体験実験「大学の研究室で身近な細菌について一緒に調べてみよう!」
佐藤拓一, 涌井杏奈, 佐野拓人, 河内美帆, 阿部峰士, 関口未来, 今井真奈美, 加藤優希, 佐藤遥菜, 岡部璃佳, 成瀬悠香, 久保七海, 三角彩華
新潟大学 附属学校部・教育学部・教職大学院・全学教職支援センター合同FD「附属校園✕大学 研究交流ポスターセッション」(新潟) 2025.12
スマートフォン/タブレット画面に付着・生息する細菌:手指との比較 Invited
五十嵐優翔, 伊藤菜々, 小田直太郎, 加藤優希, 阿部峰士, 涌井杏奈, 佐藤拓一
令和7年度N-Step新潟・成果発表会(新潟) 2025.12
使用済みの機能性不織布マスクから検出された細菌の分析 Invited
小田直太郎, 阿部峰士, 関口未来, 加藤優希, 涌井杏奈, 佐藤拓一
令和7年度N-Step新潟・成果発表会(新潟) 2025.12
Microbiota profiling on the surface of elevator buttons and fingers International conference
Kato Y., Wakui A., Sekiguchi M., Kawachi M., Sato H., Naruse Y., Kubo N., Misumi A., Sano H., Abiko Y., Washio J., Sato T.
IADR APR 2025 (New Delhi, India) 2025.9
Microbiota profiling of the remaining milk black-tea and coffee bottled-beverages International conference
Kawachi M., Sato H., Wakui A., Kato Y., Sano H., Abiko Y., Washio J., Sato T.
IADR APR 2025 (New Delhi, India) 2025.9
Microbiota profiling of the remaining bottled black tea and coffee beverages
Kawachi M., Sato H., Wakui A., Kato Y., Sano H., Abiko Y., Washio J., Sato T.
The Update Symposium in the 67th Annual Meeting of the JAOB (Kita-kyushu) 2025.9
Microbiota profiling of the remaining bottled black tea and coffee beverages: Effects of adding milk International conference
Sato H., Kawachi M., Wakui A., Sekiguchi M., Kato Y., Sano H., Abiko Y., Washio J., Takahashi N., Sato T.
Interface Summer Seminar 2025 (The 20th International Workshop on Biomaterials in Interface Science) (Sendai) 2025.8
Microbiota profiles on the multifunctional non-woven fabric masks surfaces International conference
Sekiguchi M., Wakui A., Kawachi M., Sato H., Kato Y., Sano H., Abiko Y., Washio J., Takahashi N., Sato T.
Interface Summer Seminar 2025 (The 20th International Workshop on Biomaterials in Interface Science) (Sendai) 2025.8
Profiling of microbiota in catechin-enriched green tea bottled beverages International conference
Imai M., Kawachi M., Wakui A., Sekiguchi M., Sato H., Kato Y., Naruse Y., Asano N., Morohashi M., Sano H., Abiko Y., Washio J., Sato T.
The 103rd Annual Meeting of the IADR (Barcelona, Spain) 2025.6
Profiling of microbiota on the multifunctional non-woven fabric masks surfaces International conference
Sekiguchi M., Sano H., Wakui A., Kawachi M., Imai M., Kato Y., Sato H., Naruse Y., Abiko Y., Washio J., Sato T.
The 103rd Annual Meeting of the IADR (Barcelona, Spain) 2025.6
臨床検査学分野の若手女性研究者が行っている細菌学研究の一例紹介 Invited
涌井杏奈, 佐藤拓一
第98回日本細菌学会総会(金沢)ワークショップ「臨床検査学分野の細菌学研究」 2025.5
スマートフォン画面から検出された細菌の分析
五十嵐優翔, 小田直太郎, 加藤優希, 涌井杏奈, 佐藤拓一
第98回日本細菌学会総会(金沢)「中・高校生研究発表セッション」 2025.5
使用済みの機能性不織布マスクから検出された細菌の分析
小田直太郎, 加藤優希, 関口未来, 涌井杏奈, 佐藤拓一
第98回日本細菌学会総会(金沢)「中・高校生研究発表セッション」 2025.5
保健学研究科における外国人留学生の獲得とグローバル人材育成の実績・課題・将来 〜横展開の可能性に向けて〜 Invited
佐藤拓一
第3回グローバル推進機構FD・SD「国際化はキャンパス全体の挑戦だ!〜医歯学系からはじまる学内連携未来戦略〜」 2025.5 新潟大学グローバル推進機構
博士前期課程および学部学生による、海外で現地開催される国際学会発表への挑戦(その2) Invited
佐藤拓一
令和6年度 新潟大学全学FD・SD 主体的学修を推進する新潟大学の取り組み(学長教育助成制度成果報告会) 2025.3
保健学研究科におけるグローバル対応力養成教育の取り組みについて Invited
佐藤拓一
令和6年度 国際交流センターFD・SD「大学院におけるグローバル対応力養成教育の取り組み状況と今後の展開」 2025.1 新潟大学国際交流センター
スマホ・タブレット画面の細菌:その量および構成について, Invited
五十嵐優翔, 小田直太郎, 丸山伸吾, 阿部峰士, 加藤優希, 佐藤 拓一
令和6年度N-Step新潟(ジャンピングステージ)研究発表会(新潟) 2024.12
Profiling of microbiota in the remaining bottled unsweetened tea and coffee beverages
Miyazawa M, Kawachi M, Wakui A, Imai M, Sano H, Abiko Y, Washio J, Takahashi N, Sato T
The Update Symposium in the 66th Annual Meeting of the JAOB (Nagasaki) 2024.11
Microbiological profiling of the surfaces of used-masks: Effects of non-woven fabric mask sprays International conference
Abe T, Wakui A, Sekiguchi M, Miyazawa M, Sato A, Kawachi M, Imai M, Maruyama, S, Sano H, Sato T
The 16th Japan-Korea International Symposium on Microbiology (Sapporo) 2024.8
Molecular microbiological profiling of green tea bottled beverages International conference
Kato Y, Wakui A, Miyazawa M, Kawachi M, Abe T, Sato A, Imai M, Sato H, Okabe R, Sato T
The 16th Japan-Korea International Symposium on Microbiology (Sapporo) 2024.8
使用済みマスクに付着・生息する細菌の分析:細菌の量および構成から不織布マスクの再利用を考える
小田直太郎, 阿部峰士, 丸山伸吾, 涌井杏奈, 佐藤拓一
第97回日本細菌学会総会(中・高校生研究発表セッション)(札幌) 2024.8
Profiling of microbiota in the remaining unsweetened tea and coffee drinks in plastic bottles International conference
Miyazawa M, Kawachi M, Wakui A, Imai M, Sato H, Kato Y, Okabe R, Naruse Y, Sato N, Asano N, Morohashi M, Abe T, Sato A, Sano H, Abiko Y, Washio J, Takahashi N, Sato T
The 19th International Workshop on Biomaterials in Interface Science (Interface Summer Seminar 2024) (Sendai) 2024.8
Molecular microbiological profiling of the tongue before/after tongue brushing International conference
Sato A, Miyazawa M, Abe T, Kaku N, Imai M, Takahashi N, Kawachi M, Wakui A, Washio J, Sato T
The 102nd General Session of the IADR (New Orleans, LA, USA) 2024.3
Microbiological profiling in the remaining tea/coffee drinks in plastic bottles International conference
Miyazawa M, Kawachi M, Wakui A, Kaku N, Takahashi N, Abe T, Sato A, Imai M, Arai H, Sato H, Kato Y, Okabe R, Naruse Y, Sato N, Abiko Y, Washio J, Sato T
The 102nd General Session of the IADR (New Orleans, LA, USA) 2024.3
博士前期課程および学部学生による、海外で現地開催される国際学会発表への挑戦:オンラインでは味わえないリアルな学会発表体験 Invited
佐藤拓一
令和5年度 新潟大学全学FD・SD 主体的学修を推進する新潟大学の取り組み(学長教育助成制度成果報告会) 2024.3
Profiling of the microbiota in the remaining green tea of the plastic bottles
Kawachi M, Wakui A, Sano H, Abiko Y, Washio J, Takahashi N, Sato T
The Update Symposium 2 in the 65th Annual Meeting of the JAOB (Tokyo) 2023.9
Profiling of the microbiota on the surfaces of non-woven fabric masks after wearing and smartphone touchscreens International conference
Wakui A, Kawachi M, Takahashi N, Abe T, Maruyama S, Kaku N, Miyazawa M, Sato A, Imai M, Arai H, Tanaka K, Abiko Y, Washio J, Takahashi N, Sato T
The 18th International Workshop on Biomaterials in Interface Science (Interface Summer Seminar 2023) (Sendai) 2023.8
Profiling of the microbiota in the remaining green tea drinks in plastic bottles International conference
Kawachi M, Wakui A, Kaku N, Takahashi N, Miyazawa M, Abe T, Sato A, Imai M, Arai H, Sato H, Kato Y, Okabe R, Naruse Y, Sato N, Tanaka K, Higuchi M, Abiko Y, Washio J, Takahashi N, Sato T
The 18th International Workshop on Biomaterials in Interface Science (Interface Summer Seminar 2023) (Sendai) 2023.8
飲みかけのペットボトル飲料物(清涼飲料水)および飲み口から検出される細菌のプロファイリング
河内美帆, 高橋七瀬, 賀来ながら, 樋口真由, 涌井杏奈, 佐藤拓一
第61回日本臨床化学会年次学術集会(学生シンポジウム)福岡 2021.11
手洗い前後の手指から検出される皮膚常在菌叢のプロファイリング:細菌学的・分子生物学的解析
高橋七瀬, 賀来ながら, 樋口真由, 涌井杏奈, 佐藤拓一
第61回日本臨床化学会年次学術集会(学生シンポジウム)福岡 2021.11
Profiling of microbiota in various drinks of plastic bottle after drinking directly from plastic bottles
Kawachi M, Takahashi N, Kaku N, Higuchi M, Wakui A, Washio J, Takahashi N, Sato T
The Update Symposium in the 63rd Annual Meeting of the JAOB (Zoom) 2021.10
PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム
涌井杏奈, 佐野拓人, 河内美帆, 丸山伸吾, 枡田菜々子, 佐藤拓一
第60回日本臨床化学会年次学術集会(学生シンポジウム;オンライン) 2020.11
Profiling of microbiota of baby-drinks after drinking with artificial nipples
Wakui A, Sano H, Kawachi M, Maruyama S, Masuda N, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Okada Y, Sato T
The Update Symposium in the 62nd Annual Meeting of the JAOB 2020.9
Profiling of microbiota in baby-drinks and liquid baby formula consumed with an artificial nipple
Wakui A, Sano H, Kawachi M, Washio J, Abiko Y, Mayanagi G, Yamaki K, Takahashi N, Sato T
The Update Symposium in the 61st Annual Meeting of the JAOB 2019.10
口腔から乳児用飲料物内への流入:ニプルを通して
涌井杏奈, 佐野拓人, 河内美帆, 佐藤拓一
第59回日本臨床化学会年次学術集会(学生シンポジウム)仙台 2019.9
母乳中の細菌叢解析:授乳による影響について Invited
佐野拓人, 涌井杏奈, 河内美帆, 佐藤拓一
第59回日本臨床化学会年次学術集会(学生シンポジウム)仙台 2019.9
液体ミルクの安全性の立証に向けて:飲料物の飲み口に付着する細菌の量および構成の解析 Invited
涌井杏奈, 平吹有香, 佐野拓人, 曾田彩花, 佐藤拓一
第57回日本臨床化学会年次学術集会(学生シンポジウム)札幌 2017.10
メタボローム解析:口腔疾患の発生機序解明への新たなアプローチ Invited International coauthorship
鷲尾純平, 小川珠生, 北村 淳, 森島浩允, Tian Lingyang, 石黒和子, 真柳 弦, 安彦友希, 佐藤拓一, 高橋信博
第68回東北大学歯学会 インターフェイス口腔健康科学研究紹介 2015.12
PCR-dipstick DNA chromatography for multiplex analysis of oral microbiota International coauthorship
Tian L, Sato T, Niwa K, Mayanagi G, Yamaki K, Kawase M, Tanner ACR, Takahashi N
The Satellite Symposium on The 56th Annual Meeting of the JAOB 2014.9
Oral microbiota in crevices around dental implants: profiling of the oral biofilm Invited International coauthorship International conference
Sato T, Kawamura Y, Mayanagi G, Washio J, Takahashi N
Innovative Research for Biosis-Abiosis Intelligent Interface Symposium: The 5th International Symposium for Interface Oral Health Science 2014.1
Microbiota profiling of bronchial fluids of elderly patients Invited
Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Takahashi N
2013.9
Tanda N, Ishida N, Hoshikawa Y, Sato T, Takahashi N, Hosokawa R, Koseki T
Hydrogen sulfide, methyl mercaptan, acetaldehyde in oral health care for perioperative, patients with, pulmonary carcinoma
The Satellite Symposium on The 55th Annual Meeting of the JAOB 2013.9
Molecular biological profiling of oral biofilm: Quantitative and qualitative analyses Invited International conference
Sato T, Kawamura Y, Ishida N, Yamaki K, Matsuyama J, Takahashi N
The 2nd IADR-APR (Asia Pacific Region): JADR Mini-Symposium 2013.8
Profiling of oral biofilm microbiota utilizing molecular biological methods Invited International conference
Sato T, Kawamura Y, Yamaki K, Shimauchi H, Takahashi N
Peking-Tohoku Dental Symposium: Innovative Research for Biosis-Abiosis Intelligent Interface 2013.7
口腔フローラのプロファイリング Invited
佐藤拓一, 安彦友希, 長谷川彩子, 真柳 弦, 鷲尾純平, 中條和子, 堀(宮澤)はるみ, 高橋信博
第58回東北大学歯学会 インターフェイス口腔健康科学研究紹介 2010.12
高齢者の歯肉縁下プラークバイオフィルム微生物叢の特徴 Invited
安彦友希, 佐藤拓一, 高橋信博
文科省特別経費対象事業「歯学連携ネットワークによる口腔からQOL向上を目指す研究」第2回「口腔環境制御研究」カテゴリー集会 2010.2
口腔細菌叢の重要性:研究の現在と未来 Invited
佐藤拓一
第47回歯科基礎医学会学術大会 サテライトシンポジウム「口腔微生物叢研究の新展開」 2005.9
口腔細菌叢の網羅的解析について
佐藤拓一, 高橋信博
第18回口腔嫌気性菌研究会 2003.9
FLAGs事業を活用した国際会議参加費及び旅費支援
2026.11 新潟大学
今井真奈美
給付型奨学金(一般財団法人)東洋水産財団 森記念奨学金
2026.8
久保七海
(独立行政法人)日本学生支援機構(第一種貸与型奨学金)返還免除
2026.7
今井真奈美
RA費(新潟大学大学院博士後期課程)
2026.7 ワセリンリップ塗布が口唇表面上の微生物バイオフィルムに与える影響(網羅的・分子生物学的解析)
今井真奈美
大学院生・国際誌掲載奨励プログラム(論文投稿支援)
2026.7 新潟大学
今井真奈美, 佐藤拓一
学長による学生個人表彰(新潟大学学生表彰に関する規程に基づく表彰)
2026.3
河内美帆
新潟大学 大学院博士後期課程(保健学研究科)代表@卒業式
2026.3
河内美帆
奨学賞(一般社団法人)大学女性協会 新潟支部 2025年度(第76回 新潟支部)
2025.11 「ジェンダー平等社会実現のためにすべきこと~女性の自立:大学院において女性が活躍できる進路を考える~」
河内美帆
最優秀発表賞(新潟大学院生会第5回2025年度研究発表会)
2025.11 「使用済み機能性マスク上の細菌 vs. 口唇上の細菌(バイオフィルムの分子生物学的解析)」
関口未来
RA費(新潟大学大学院博士後期課程)
2025.9 飲料保管に関する食品科学的・微生物学的研究(網羅的・分子生物学的解析)
河内美帆
新潟大学 大学院生・国際誌掲載奨励プログラム(論文投稿支援)
2025.9
賀来ながら, 高橋七瀬, 佐藤拓一
給付型奨学金(公益財団法人)佐々木環境技術振興財団
2025.7 スマートフォン画面やエレベータボタンの微生物プロファイリング:感染・伝播の可能性を探る
加藤優希
(独立行政法人)日本学生支援機構(第一種貸与型奨学金)返還免除
2025.7
阿部峰士, 佐藤 彩
給付型奨学金(公益財団法人)安藤スポーツ・食文化振興財団
2025.6 ペットボトル飲料の安全衛生に関する食品科学的・分子微生物学的アプローチ
加藤優希
学生ポスター発表奨励賞(第74回日本口腔衛生学会学術大会)
2025.5 「ミルク添加のペットボトル珈琲および紅茶飲料の微生物プロファイリング」
成瀬悠香, 河内美帆, 佐藤遥菜, 涌井杏奈
「最優秀賞」令和6年度 国立大学臨床検査学系博士後期課程
2025.3
河内美帆
(公益財団法人)ロッテ財団 研究論文作成・掲載・発表経費助成
2025.3 Microbiota of breast milk and liquid baby-formula after artificial-nipple feeding
涌井杏奈
新潟大学大学院博士前期課程(保健学研究科)代表@卒業式
2025.3
宮沢美里
奨学賞(一般社団法人)大学女性協会 新潟支部 2024年度(第75回 新潟支部)
2024.12 「ジェンダー平等社会実現のためにすべきこと~Z世代の観点から考える学業・雇用形態・家庭のあり方~」
宮沢美里
優秀発表賞(新潟大学院生会第4回2024年度研究発表会)
2024.12 「濃い茶ペットボトル飲料内の微生物プロファイリング:スクリーニング実験」
今井真奈美, 岡部璃佳, 宮沢美里, 河内美帆, 涌井杏奈
「若手賞」日本分析化学会関東支部新潟地区部会
2024.11 The effect of formalin-fixed paraffin embedded process on salivary microbiota profiling
佐野拓人
学生研究奨励賞
2024.9 第77回伝統食品研究会 「市販の緑茶内の微生物プロファイリング:ペットボトル飲料を口を付けて飲んだ場合を想定したモデル実験」
加藤優希, 宮沢美里, 河内美帆, 涌井杏奈
RA費(新潟大学大学院博士後期課程)
2024.9 新生児用の飲料を含めた飲料物の保管に関する食品科学的・微生物学的研究(網羅的・分子生物学的解析)
河内美帆
(独立行政法人)日本学生支援機構(第一種貸与型奨学金)返還免除
2024.7
高橋七瀬
JST(新・次世代)未来社会を牽引するグローバルな総合知を備えたフロントランナー育成プロジェクト
2024.4 不織布マスクの微生物プロファイリング:メタゲノム、メタボローム解析
丸山伸吾
給付型奨学金(公益財団法人)尚志社
2024.4 カテキン増量の緑茶ペットボトル飲料内の微生物について:分子生物学的プロファイリング
今井真奈美
YIA(Young Investigator Award,若手研究者賞)2023年度日本臨床化学会
2023.10 搾乳母乳と新生児口腔細菌叢との連関:網羅的・分子生物学的アプローチ
涌井杏奈
JST 科学技術イノベーション創出に向けた大学フェローシップ創設事業(欠員補充)
2023.10 不織布マスクの微生物プロファイリング:メタゲノム、メタボローム解析
丸山伸吾
RA費(新潟大学大学院博士後期課程)
2023.9 新生児用の飲料を含めた飲料物の保管に関する食品科学的・微生物学的研究(網羅的・分子生物学的解析)
河内美帆
(独立行政法人)日本学生支援機構(第一種貸与型奨学金)返還免除
2023.7
涌井杏奈, 河内美帆
「優秀賞」令和4年度 国立大学臨床検査学系博士後期課程
2023.5
涌井杏奈
新潟大学大学院博士課程(進学一時)奨学金
2023.4 ペットボトル飲料物の保管に関する食品科学的・細菌学的研究(河内美帆),使用済み不織布マスクの再活用を微生物学的観点から考察する(丸山伸吾)
河内美帆, 丸山伸吾
特別賞(奨励賞) 新潟ジュニアドクター育成塾ドクタープログラム
2023.3 「使用済みマスクに生息する細菌の分析~細菌の量からマスクの再利用の可能性を考える~」
小田直太郎, 阿部峰士, 丸山伸吾, 佐藤拓一
優秀賞 令和4年度新潟大学大学院保健学研究科研究奨励金 研究成果発表会
2023.3 「母乳の細菌学的探索:保管方法に関する新たな提案の試み」
涌井杏奈
奨学賞(一般社団法人)大学女性協会 新潟支部 2022年度(第73回 新潟支部)
2022.12 「ジェンダー平等社会への思い~保健学研究を5年間経験する中で~」
涌井杏奈
(公益財団法人)ロッテ財団 第10回奨励研究助成(B)
2022.12 母乳を含めた新生児用の飲料と口腔細菌叢との連関(網羅的・分子生物学的解析)
涌井杏奈
特別賞(アイディア賞)ジュニアドクター育成塾 サイエンスカンファレンス2022
2022.11 「使用済みマスクに生息する細菌の分析~細菌の量からマスクの再利用を考える~」
小田直太郎, 阿部峰士, 丸山伸吾, 佐藤拓一
最優秀賞(新潟大学院生会第2回2022年度研究発表会)
2022.11 「飲みかけの緑茶ペットボトルの細菌のプロファイリング」
加藤優希, 宮沢美里, 河内美帆, 涌井杏奈, 賀来ながら, 佐藤 彩, 阿部峰士
RA費(新潟大学大学院博士後期課程)
2022.9 母乳を含めた新生児用の飲料と口腔細菌叢との連関(網羅的・分子生物学的解析)
涌井杏奈
給付型奨学金(一般財団法人)東洋水産財団 森記念奨学金
2022.8
加藤優希
新潟大学博士育成のための論文投稿支援
2022.7 Bacterial concentration and composition in liquid baby formula and a baby drink consumed with an artificial nipple
涌井杏奈, 佐藤拓一
給付型奨学金(公益財団法人)尚志社
2022.4
高橋七瀬
学長による学生個人表彰(新潟大学学生表彰に関する規程に基づく表彰)
2022.3
涌井杏奈
奨学賞(一般社団法人)大学女性協会 新潟支部 2021年度(第72回 新潟支部)
2021.12 コロナ禍を通してジェンダー平等社会を考える ~「諦める」から「挑戦する」へ:保健学研究を目指すスタートラインに立って~
高橋七瀬
2021.11 飲みかけのペットボトル飲料物(清涼飲料水)および飲み口から検出される細菌のプロファイリング
河内美帆
2021.11 手洗い前後の手指から検出される皮膚常在菌叢のプロファイリング:細菌学的・分子生物学的解析
高橋七瀬, 関沢有芽
RA費(新潟大学大学院博士後期課程)
2021.9
佐野拓人
新潟大学論文投稿支援事業
2021.7 Profiling of microbiota at the mouth of bottles and in remaining tea after drinking directly from plastic bottles of tea(涌井杏奈),Profiling system of oral microbiota utilizing polymerase chain reaction-restriction fragment length polymorphism analysis(佐野拓人)
涌井杏奈, 佐野拓人, 佐藤拓一
2020.11 PCR-RFLP法を用いた口腔内細菌叢解析(プロファイリング)システム
涌井杏奈, 佐野拓人
審査員特別賞(ジュニアドクター育成塾 サイエンスカンファレンス2020 Web開催)
2020.11 飲みかけのペットボトル飲料(お茶、スポーツ飲料、コーラ、オレンジジュース)に生息する細菌について
畑 歩花, 本間沙也花, 河内美帆, 関沢有芽, 佐藤拓一
RA費(新潟大学大学院博士後期課程)
2020.9
佐野拓人
トラベルアワード(第59回日本臨床化学会年次学術集会@仙台)
2019.9 口腔から乳児用飲料物内への流入:ニプルを通して
涌井杏奈, 佐野拓人
トラベルアワード(第59回日本臨床化学会年次学術集会@仙台)
2019.9 母乳中の細菌叢解析:授乳による影響について
佐野拓人, 涌井杏奈
: The Excellent Poster Award (The 5th International Symposium for Interface Oral Health Science)
2014.1 A rapid and simple detection of plaque bacteria using a novel molecular method, PCR-PAS, and its clinical application
東北大学総長賞(大学院歯学研究科博士課程)
2011.3 高齢者の歯肉縁下プラークバイオフィルム微生物叢における歯周炎関連細菌の定量的解析
安彦友希
東北大学大学院修士課程(歯学研究科)総代
2007.3 定量PCR法による歯肉縁下プラークバイオフィルムの多様性解析
安彦友希
日本歯科保存学会奨励賞
2006 Nested PCR法を用いた歯垢細菌叢のプロファイリング
真柳 弦
東北大学大学院博士課程(歯学研究科)総代
2005.3 Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects
真柳 弦
臨床化学研究室OPEN LAB:大学研究室で体験する分子生物学最前線「遺伝子解析・操作技術の実践教育プログラム」
2026.6 - 2027.3
Research category:大学魅力向上支援事業補助金
Awarding organization:新潟県
佐藤拓一
Authorship:Principal investigator Grant type:Competitive
Grant amount:\500000 ( Direct Cost: \500000 )
大学で本格的に学ぶ微生物学・細菌学に関する先取り体験・研究活動
2024.9 - 2025.3
Research category:新潟県大学魅力向上支援事業補助金
佐藤 拓一
Authorship:Principal investigator Grant type:Competitive
Grant amount:\500000 ( Direct Cost: \500000 )
口腔マイクロバイオームの新・存在意義~全身・口腔健康に寄与する亜硝酸産生の全容~
Grant number:24K02657
2024.4 - 2028.3
System name:科学研究費助成事業
Research category:基盤研究(B)
Awarding organization:日本学術振興会
鷲尾 純平, 高橋 信博, 安彦 友希, 互野 亮, 佐藤 拓一, 柳田 保子
Grant amount:\18460000 ( Direct Cost: \14200000 、 Indirect Cost:\4260000 )
不織布マスクおよびタブレット画面の微生物プロファイリング:伝播の可能性を探る
2024.4 - 2027.3
System name:日本学術振興会 科学研究費助成事業
Research category:基盤研究(B)
佐藤拓一, 坂下玲子, 田中香お里, 河村好章, 鷲尾純平, 安彦友希, 佐野拓人, 富田洋介
Authorship:Principal investigator Grant type:Competitive
Grant amount:\18330000 ( Direct Cost: \14100000 、 Indirect Cost:\4230000 )
口腔マイクロバイオームの新機能:「副代謝系」の解明による口腔と全身の健康増進戦略
Grant number:23K21499
2024.4 - 2025.3
System name:科学研究費助成事業
Research category:基盤研究(B)
Awarding organization:日本学術振興会
高橋 信博, 佐藤 拓一, 鷲尾 純平, 坂本 光央
Grant amount:\3120000 ( Direct Cost: \2400000 、 Indirect Cost:\720000 )
博士前期課程および学部学生による、海外で現地開催される国際学会発表への挑戦
2023.12 - 2026.3
System name:新潟大学・学長教育助成制度
佐藤拓一, 涌井杏奈, 佐野拓人, 河内美帆
Authorship:Principal investigator Grant type:Competitive
Grant amount:\1200000 ( Direct Cost: \1200000 )
大学の研究室で身近な細菌について一緒に調べてみよう!
2023.7 - 2027.3
System name:科学技術知識普及事業費助成
Awarding organization:(公財)内田エネルギー科学振興財団
佐藤拓一, 涌井杏奈, 佐野拓人, 河内美帆
Authorship:Principal investigator Grant type:Competitive
Grant amount:\450000 ( Direct Cost: \450000 )
大学の研究室で身近な細菌について一緒に調べてみよう!
2023.7 - 2024.8
System name:高校生アカデミック・インターンシップ研修
Awarding organization:新潟県教育委員会
佐藤拓一
Authorship:Principal investigator Grant type:Collaborative (industry/university)
Grant amount:\362000 ( Direct Cost: \362000 )
大学の研究室で身近な微生物について一緒に調べてみよう!
2023.4 - 2027.3
System name:子どもゆめ基金 科学体験活動
Awarding organization:(独)国立青少年教育振興機構
佐藤拓一, 涌井杏奈, 佐野拓人, 河内美帆
Authorship:Principal investigator Grant type:Competitive
Grant amount:\1300250 ( Direct Cost: \1300250 )
女子学生の博士後期課程への進学プロモーション
2022.4 - 2026.3
System name:新潟大学・ジェンダーダイバーシティ部局自律化・地域共創プロジェクト
佐藤拓一
Authorship:Principal investigator Grant type:Competitive
Grant amount:\875000 ( Direct Cost: \875000 )
学生の研究活動の奨励・鼓舞を目指す!
2021.4 - 2026.3
System name:教員活性化応援制度・チャレンジマイル制度
佐藤 拓一
Authorship:Principal investigator Grant type:Competitive
Grant amount:\50000 ( Direct Cost: \50000 )
Relationship between breast milk & artificial formula and neonatal oral microbiota: Metagenomic and metabolomic analyses
Grant number:21H03247
2021.4 - 2025.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
Authorship:Principal investigator Grant type:Competitive
Grant amount:\17160000 ( Direct Cost: \13200000 、 Indirect Cost:\3960000 )
口腔マイクロバイオームの新機能:「副代謝系」の解明による口腔と全身の健康増進戦略
Grant number:21H03151
2021.4 - 2025.3
System name:科学研究費助成事業
Research category:基盤研究(B)
Awarding organization:日本学術振興会
高橋 信博, 佐藤 拓一, 鷲尾 純平, 坂本 光央
Authorship:Coinvestigator(s) Grant type:Competitive
Grant amount:\17810000 ( Direct Cost: \13700000 、 Indirect Cost:\4110000 )
メタ16S解析を用いた帝王切開出生児の口腔内細菌叢獲得機序の解明
Grant number:21K10266
2021.4 - 2024.3
System name:科学研究費助成事業 基盤研究(C)
Research category:基盤研究(C)
Awarding organization:日本学術振興会
佐野拓人, 岡田康男, 佐藤拓一
Authorship:Coinvestigator(s) Grant type:Competitive
Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )
帝王切開にて出生した新生児では腸内細菌叢および口腔内細菌叢に構成異常(dysbiosis)が起こることが報告されている。新生児期のdysbiosisは免疫系の発達不全や代謝系へ長期的な変化を引き起こすことが示されている。口腔内細菌叢においては帝王切開児では自然分娩児に比べて、早い時期からう蝕関連菌を獲得すること、幼児期う蝕のリスクが上がることが報告されている。口腔は消化管への門戸でもあり、細菌叢の口腸連関も示されていることから、口腔 dysbiosis は腸内細菌叢の撹乱、ひいては新生児の正常な発達にも密接に関連する問題である。本研究では分娩形式に関連した新生児口腔dysbiosisの発生プロセス及び、新生児の正常な口腔内細菌叢形成とは何かを明らかにするために、経時的に新生児口腔内細菌を質的(細菌叢構成)かつ量的(細菌量変動)に解析する。
本年度は新生児の口腔内細菌叢を解析するためのセットアップを行った。ヒト唾液から分離された細菌59種に関して、細菌の分類に用いられる16S rRNA遺伝子をPCR法で増幅し、制限酵素で切断後、アガロース電気泳動した像(PCR-RFLP像)を収集した。制限酵素であるHpaIIを用いて得られるPCR-RFLP像が、多くの口腔内細菌の分類に有用であることを明らかにし、成果を論文として公表した。新生児の口腔内擦過サンプルの採取にも着手しており、我々の構築したPCR-RFLP像を用いた細菌叢解析法を利用して細菌叢構成と細菌量変動の解析を行なっている。加えて、ヒト母乳には特有の細菌叢が存在していることが近年の研究で明らかになっていることから、本研究ではこのことにも配慮し、授乳形式(母乳育児・母乳と人工乳の混合育児・人工乳育児)によっても被験者を分類し、母乳と口腔内細菌叢形成の関連についても検討を行なっている。
新視点で探る口腔バイオフィルム細菌叢の存在意義~硝酸還元活性と全身・口腔健康~
Grant number:20K10241
2020.4 - 2023.3
System name:科学研究費助成事業 基盤研究(C)
Research category:基盤研究(C)
Awarding organization:日本学術振興会
鷲尾 純平, 佐藤 拓一, 高橋 信博, 互野 亮
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
口腔バイオフィルムは、これまでう蝕、歯周病、誤嚥性肺炎等の様々な病原因子としての“負”の作用がクローズアップされてきた。一方、構成細菌の持する硝酸還元活性により産生される亜硝酸塩は、血液循環の改善や、一部の口腔細菌の増殖を抑制することが近年示され、我々の健康維持に寄与しうるという“正”の作用を持つ可能性に注目が集まっている。
そこで私たちはこれまで、口腔バイオフィルム中の硝酸還元活性を持つ菌について網羅的探索を行い、口腔内における亜硝酸産生を担う細菌種として、Actinomyces, Shaalia, Veillonella, Neisseria, Rothia属がその大半を占めることを明らかにしてきた。
さらに、前述の細菌種のうち、いくつかを用いて、酸素濃度、pH、亜硝酸濃度、糖濃度、乳酸濃度等、種々の環境因子を変動させた際の硝酸還元活性の変化を測定した。その結果、いずれの菌においても、環境条件により、その産生能を大きく変動させることが明らかとなり、その変動は菌種によって傾向が異なることが示唆された。
本年度は、引き続きどのような条件下で最も効率的に亜硝酸が産生されるのかについて検討するとともに、亜硝酸をさらに分解する菌の探索にも着手し、いくつかの菌種で分解活性を持つことを認めた。なお、当初予定していた実際のプラーク試料をもちいた検討は、コロナの感染対策の影響により、少し計画を順延した。
次年度も引き続きこれらの点について明らかにしながら、研究を進める予定である。また、研究内容の公表に向けても取り組みたい。
日本の保健学最先端研究の体験型演習:日本とスリランカの将来構想を考える
2019.10 - 2020.3
System name:日本・アジア青少年サイエンス交流事業(さくらサイエンスプラン)
Research category:科学技術体験コース
Awarding organization:(国研)科学技術振興機構
Authorship:Principal investigator
「新潟地域ヘルスケア体制を持続可能なものとする高度化人材育成」に関する事業計画
2019.4 - 2022.3
佐藤拓一
液体ミルクの安全性の立証に向けて:飲み残したペットボトルの口の部分に付着する細菌および飲料物内の細菌の量および構成の解析
2018.1 - 2018.12
System name:一般研究奨励助成事業費助成金
Awarding organization:(公財)総合健康推進財団
佐藤拓一, 鷲尾純平
Authorship:Principal investigator Grant type:Competitive
Grant amount:\500000 ( Direct Cost: \500000 )
液体ミルクの安全性の立証に向けて:飲料物の飲み口に付着する細菌の量および構成の解析
2017.12 - 2018.11
Research category:やずや食と健康助成研究【チャレンジ部門】
佐野拓人, 佐藤拓一, 鷲尾純平
Authorship:Coinvestigator(s) Grant type:Competitive
Grant amount:\500000 ( Direct Cost: \500000 )
What is a healthy oral microbiome? Proposal of oral health indicators based on metabolic resilience
Grant number:17H04420
2017.4 - 2021.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
Takahashi Nobuhiro
Grant amount:\16900000 ( Direct Cost: \13000000 、 Indirect Cost:\3900000 )
1. The "metabolic database of oral microbiome" was expanded by newly discovering glucose metabolism of new caries-associated bacteria Bifidobacaterium and Scardvia, nitrate metabolism of Veillonella and Neisseria, and ethanol metabolism system of Streptococcus and Neisseria.
2. These new caries-related bacteria mainly produced acetic acid from sugars, and their metabolism showed high resistance to fluoride.
3. Furthermore, nitrate metabolism was biochemically coupled with lactic acid metabolism (lactic acid consumption), and the consumption of lactic acid improves the caries-induced environment, and nitrite, which is a nitrate metabolite, has an antibacterial effect. It has been clarified that multiple metabolic systems in the microbiome are coordinated with each other to bring about metabolic resilience.
Profiling microbiota of bronchial fluids in the elderly and its association with oral cavities
Grant number:17K12006
2017.4 - 2020.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Sato Takuichi
Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )
The source of the bacteria involved in silent aspiration remains to be completely defined. The purpose of the present study was to obtain reliable evidence on silent aspiration of oral bacteria in elderly patients. Our results suggest that bacteria in bronchial fluids associated with silent aspiration are derived from saliva, and that the bronchial fluids of elderly patients with an impaired swallowing reflex may have a characteristic microbiota. In addition, our results suggest that oral hygiene is important to reduce silent aspiration and to subsequently prevent the occurrence of aspiration pneumonia in elderly patients.
感染根管フローラの構造と機能解析-バイオインフォマティクスの歯内治療への応用
Grant number:17K11698
2017.4 - 2020.3
System name:科学研究費助成事業 基盤研究(C)
Research category:基盤研究(C)
Awarding organization:日本学術振興会
八巻 惠子, 佐藤 拓一, 鷲尾 純平
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
根尖性歯周炎の原因は根管内の細菌感染である。しかし感染根管から検出される細菌は多種多様で、「根尖性歯周炎の病原菌」はいまだ特定されておらず、根管内細菌の総量や、細菌集合体であるフローラとして病原性を発揮している可能性がある。本研究では根管内フローラをパンゲノムとして捉え、その構造や機能特性を追及し、口腔内細菌が根管内に侵入、根管壁象牙質に感染し、根尖歯周組織を傷害するメカニズムの解明に努めた。
感染根管治療が必要で東北大学病院の歯内療法科・歯周病科を受診した患者からインフォームドコンセントを得て根管壁象牙質削片を採取、細菌学的に検索した。解析した3例は、いずれも術前に強い根尖部圧痛を有していたが、1例からは実数カウントでも嫌気培養でも細菌を全く検出できなかった。この症例は髄角に近接したレジン充填による慢性閉鎖性歯髄炎による処置後数十年経って根尖歯周組織に炎症を誘発したものの、直前まで歯髄が生活していたため根管内への細菌侵入が起こらなかったと推測された。残り2例は1mL中の生菌数が4.03~5.65×10の4乗と高値で、Parvimonas micraが共通して検出された。うち1例ではMogibacterium timidumも検出され、P. micraとM. timidumは根尖部に急性炎症症状を伴う根管から高い頻度で検出され、かつ両者が共存していることも多い、というこれまでの我々の研究調査で認められた傾向と合致していた。
一方、飲みかけのペットボトル容器の蓋および容器内に残った飲料を細菌学的に検索したところ、最初は唾液と似た細菌構成であったが、翌日にはStreptococcus属のみとなり、付着・侵入した細菌間で競合・淘汰が起きることがわかった。これにより、感染根管内でも侵入細菌が競合淘汰して固有のフローラへと成熟していく可能性が示唆された。
How is the nitrite contributing to our general and oral health promotion produced in oral microflora?
Grant number:17K12003
2017.4 - 2020.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
WASHIO JUMPEI
Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )
In recent years, it attracts attention that oral bacteria can metabolize nitrate included in yellow-green vegetables or saliva to nitrite, that has antibacterial activity and vasodilatation effect, and which may contribute to maintain our general and oral health.However, the details about the production of nitrite in the oral cavity is still unknown. Therefore, we tried to clarify the whole aspect of nitrite production in the oral cavity.
The nitrite-producing activity in the oral cavity was higher in dental plaque than tongue coatings. The interindividual differences was also higher. It was clarified that the bacterial species such as Actinomyces, Shaalia, and Veillonella species known as commensal bacteria mainly contribute to the nitrite production in oral cavity. In addition, it was also clarified that those nitrite-producing activity can be fluctuated by the various environmental factors such as an oxygen concentration, pH and the lactate concentration.
Developing the model using pH/calcium fluorescent imaging for prevention and treatment of dental caries
Grant number:16K11540
2016.4 - 2020.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MAYANAGI Gen
Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )
This study aimed to develop the methods to assess pH change at the interface between material or tooth and bacteria continuously under the same kind of conditions as an oral microenvironment in vitro. The fluoride releasing materials and the silver diamine fluoride coated tooth inhibited pH fall at the interface between material/tooth and bacteria. The degree of inhibition of pH fall seemed to correspond to the amounts of fluoride and silver detected, suggesting that the fluoride and silver released from these materials inhibited pH fall.
Development of a life enhancement model focusing on dining for home care elderly patients
Grant number:15H05098
2015.4 - 2018.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
Sakashita Reiko, ONO Hiroshi, HAMADA Misao, NISHIHIRA Tomoko, FUJIWARA Miho
Grant amount:\19110000 ( Direct Cost: \14700000 、 Indirect Cost:\4410000 )
(1)Development of a monitoring system of eating and swallowing focused on a tongue movement: Surface EMGs around the lower jaw were taken at the time of the pronunciation. Based on the neural network analysis, a novel monitoring system was developed.(2)Development of a life support model for elderly patients: It was clarified what kind of care was done for supporting good dining by visiting nurses. Based on those findings, a model was developed. (3)Trials and evaluations of a life enhancement model: programs were developed and carried out for six months to improve dining for home care elderly patients, family members and visiting nurses based on the model created in (2). The effects were evaluated both three- and six-months later. In addition to the model proposed in (2), it was clarified that the processes of dialogue by teams and thinking about care centering on the patients enhance patients' abilities to "living themselves".
The elucidation of the pathogenic activity of periodontal disease-related bacteria by metabolomics approaches; The establishment of new method to monitor their metabolic activity in real-time
Grant number:26463154
2014.4 - 2018.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Washio Jumpei
Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )
It has been suggested that the periodontal disease-related bacterial "metabolism" was strongly related to the pathogenicity of periodontal disease. However, they were still unclear because the study about their metabolism was not done enough by technical limitation. Therefore, in this KAKENHI project, I established the new method to monitor their metabolic activity easily in real-time and improved the technical method for the metabolome analysis of periodontal disease-related bacteria. The results in this study suggested that their metabolism was affected greatly by the environmental factors and drugs in actual. These new methods and knowledges could contribute to elucidate the onset mechanism of periodontal diseases and the development of new strategy for their treatment and prevention.
Microbiota profiling of infected root canals using metagenomic analysis
Grant number:26462869
2014.4 - 2018.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Sato Takuichi
Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )
Apical periodontitis is an infectious and inflammatory disease of periapical tissues caused by oral bacteria invading the root canal system. In the present study, metagenomic analysis was performed in order to profile the microbiota of infected root canals.
Change in infected root canal microflora during the course of root canal therapy
Grant number:25861785
2013.4 - 2017.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Research category:Grant-in-Aid for Young Scientists (B)
Awarding organization:Japan Society for the Promotion of Science
Hashimoto Kazuhiro, SATO TAKUICHI
Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )
Obligate anaerobes have been reported to comprise the majority of oral biofilm including intracanal-microbiota. The bacterial profiling of infected root canals has been long performed. In the present study, metagenomic analysis was applied to the profiling of microbiota in infected root canals, and detailed components of the microbiota of infected root canals were found utilizing high-speed sequencers without random-cloning.
Evaluation of intracanal biota using metagenomics - a new strategy for endodontic treatment
Grant number:25462945
2013.4 - 2016.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
YAMAKI KEIKO, SATO TAKUICHI, MAYANAGI GEN, SHIMAUCHI HIDETOSHI
Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )
Intracanal microflora associated with apical periodontitis was investigated. Under informed consent of patients, dentin samples were collected and analyzed using two rapid microbial cell counters, anaerobic culture, and PCR targeting 16S rRNA genes. Bacterial species found from endodontically suffering canals showed a great diversity in both quality and quantity. Mechanical enlargement of the root canal combined with chemical disinfection could effectively reduce the pre-dominantly anaerobic pathogen. Propionibacterium sp. and Olsenella sp. were frequently recovered in contrast to previous reports. Evaluating the difference in bacterial profile between oral cavity and root canal through metagenomics could lead to a new strategy for diagnosis and treatment of apical periodontitis.
Multifaceted approach to the analyses of dental plaque biofilm targeting dental caries-associated bacteria, and its application to dental caries prevention
Grant number:25463237
2013.4 - 2016.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Matsuyama Junko, SATO Takuichi, WASHIO Jumpei, TAKENAKA Shoji, OHSHIMA Hayato
Grant amount:\5070000 ( Direct Cost: \3900000 、 Indirect Cost:\1170000 )
As an animal model of dental caries, mice have been generally utilized. In this study, dental plaque microbiota of pre- and post-weanling, were analyzed by using molecular biological techniques. Enterococcus, Escherichia, Lactobacillus and Lactococcus were predominant. From the findings of the present study, the bacterial composition of dental plaque microbiota of mice varies markedly from that of humans (in which Streptococcus, Actinomyces and Veillonella are predominant); and this may be due to differences in the daily diet.
Novel roles of antimicrobials in the process of pulpal regeneration: its relationship with dendritic cells and dental pulp stem cells
Grant number:25670777
2013.4 - 2015.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Research category:Grant-in-Aid for Challenging Exploratory Research
Awarding organization:Japan Society for the Promotion of Science
OHSHIMA Hayato, SATO Takuichi, MATSUYAMA Junko
Grant amount:\3770000 ( Direct Cost: \2900000 、 Indirect Cost:\870000 )
This study aimed to elucidate responses of the infected dental pulp to capping with 3Mix in mouse molars, compared with those to calcium hydroxide cement. A class I cavity was prepared on the maxillary first molars of 6-week-old mice to expose the dental pulp and maintained for 24 hours. Subsequently, the exposed pulp was capped with 3Mix in ointment (macrogol mixed with propylene glycol: MP; 3Mix group) or calcium hydroxide cement (CH group), in addition to MP alone (control group), followed by a glass ionomer cement filling. The samples were collected at intervals of 1, 2, and 3 weeks. Immunohistochemistry for nestin and Ki-67 and TUNEL assay were performed. The use of 3Mix-MP paste as a pulp-capping agent, compared with calcium hydroxide cement, positively affects the healing of infected dental pulp in mouse molars. Further studies are necessary to clarify the mechanisms eliciting pulpal responses to 3Mix-MP paste following tooth injuries and pulpal infection.
Microbiota of children with and without dental caries, Dental plaque as a biofilm and a microbial community: implications for treatment , Cultivation of previously-uncultivated oral bacteria
2013 - 2015
System name:「外国人学者等による学術講演」助成
Awarding organization:(一財)岩垂育英会
佐藤拓一
Authorship:Principal investigator
Development of a life enhancement model focusing on dining
Grant number:24390511
2012.4 - 2015.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
SAKASHITA Reiko, MASTUSHITA Kenji, SATO Takuichi, KIM Woesook, TAKAMI Miho, KAJI Hidesuke, MORIMOTO Michiko, FUJIO Yuko, ONO Hiroshi, HAMADA Misao, NISHIHIRA Tomoko, FUJIWARA Miho, TAO Motomi
Grant amount:\18720000 ( Direct Cost: \14400000 、 Indirect Cost:\4320000 )
This study aimed to create a life enhancement model focusing on dining which make the elderly rebuild their own life in welfare facilities for seniors in Japan. In 2012 the life enhancement model focusing on dining was created, in 2013 and 2014 the model was performed and evaluated to be refined and generalized.
After three months intervention, appetite, caloric intake, water intake and weight significantly increased (p<0.001-0.05), however after one year from the intervention had started, they slightly reduced. These results suggest that this model which encouraged both groups and individuals to promote their health were effective but the continuing support was important.
Profiling of Microbiota in Infected Root Canals Utilizing Metagenomic and Bioplorer Analyses: Their Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment
Grant number:23592791
2011 - 2013
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
SATO Takuichi, MAYANGI Gen, YAMAKI Keiko, KAWAMURA Yoshiaki, MATSUYAMA Junko
Grant amount:\5330000 ( Direct Cost: \4100000 、 Indirect Cost:\1230000 )
The bacterial examination has been performed during the course of the root canal treatment. In this study, metagenomic and Bioplorer (using fluorescence reagents and a membrane filter) analyses were applied to the profiling of microbiota in infected root canals, in order to evaluate the outcomes of the treatment. As a result, detailed components of the microbiota and rapid quantification of bacteria in infected root canals were found to be successfully performed utilizing these two methods.
Development of Diagnosis and Treatment Protocol for Apical Periodontitis based on the Profiling of Intra-canal Microflora
Grant number:22592112
2010 - 2012
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
YAMAKI Keiko, SATO Takuichi, MAYANAGI Gen, SHIMAUCHI Hidetoshi
Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )
Intracanal microflora with apical periodontitis was investigated using two rapid microbial cell counters, anaerobic culture, and PCR targeting 16S rRNA genes. Mechanical enlargement of the root canal combined with chemical disinfection could effectively reduce the pathogen, though some species were resistant to this conventional protocol. Also, there were species seemingly linked to apical symptom. Species-specific antimicrobial therapy might enhance minimum intervention in cases harboring such species.
The monitoring system of incipient caries, and its special attention o bacterial dynamics in dental plaque biofilm
Grant number:22592330
2010 - 2012
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MATSUYAMA Junko, TAKENAKA Syoji, SATO Takuichi
Grant amount:\3380000 ( Direct Cost: \2600000 、 Indirect Cost:\780000 )
Plaque biofilm of humans was investigated using a rapid microbial cell counters and anaerobic cultures. On the samples of around 10^5 CFU counted, the same levels of bacterial counts were observed by the anaerobic cultures. While, indigenous plaque microbiota of mice was also analysed, and as a result, Enterococcus faecalis, Escherichia coli, Lactococcus garvieae were detected as predominant bacteria.
Development of Community health promotion model focused on oral care for elderly
Grant number:21390602
2009 - 2011
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
SAKASHITA Reiko, MASTUSHITA Kenji, SATO Takuichi, KIM Woesook, ARAI Kanako, ARAKAWA Mitsue, MATSUO Kazue, KAJI Hidesuke, TAO Motomi
Grant amount:\17290000 ( Direct Cost: \13300000 、 Indirect Cost:\3990000 )
[Purpose] This study aimed to evaluate a health promotion program for the elderly to foster self-management of oral health.
[Method] The intervention program consisted of the group studies and private consultations for three months. The group study included 1) monitoring the oral condition and practicing oral self-care, 2) monitoring oral function and practicing oral exercise and 3) group discussion on continuing oral self-care. Outcomes were evaluated at the beginning, at the end, and at three months after the investigation by scores for 1) oral self-care, 2) oral condition(decayed teeth, CPI, deposits of plaque and tartar), 3) oral function(RSST, Oral Diadochokinesis), 4) QOL(SF-8 v2^<TM>, GOHAI), 5) cognitive function(MMSE-J).
[Results] Subjects consisted of 19 males and 131 females(average age, 73.6±7.3 years ; range, 60-94 years). On oral health care, subjects cleaned their teeth more often than before and the use of dental floss was significantly increased in number(p<0.001). Periodontal score, dental plaque and tartar were significantly lower after intervention(P<0.01-0.001). Oral function also improved significantly(p<0.05-0.01). Scores for oral QOL and cognitive function improved significantly(P<0.05-0.01).
[Conclusion] These results suggest that this program promotes not only oral self-care, resulting in good oral health conditions, but also improves cognitive function in the elderly.
[Ethical consideration] Informed consent was obtained from all subjects, and this study was approved by the Research Ethics Committee of University of Hyogo.
Contribution of impairment of cough and swallowing reflexes and silent aspiration of oropharyngeal bacteria to the mechanism of postoperative pneumonia
Grant number:20591661
2008 - 2010
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
HOSHIKAWA Yasushi, NODA Masafumi, SADO Tetsu, KONDO Takashi, SATO Takuichi, TAKAHASHI Nobuhiro
Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )
This study reconfirmed the close relevance of impairment of cough and swallowing reflexes to postoperative pneumonia after lung resection. We showed the bronchial fluid from patients developing postoperative pneumonia contained considerable oral anaerobic bacteria, by using oral biochemical technique. Our intensive perioperative oral care program, including professional assessment of oral status, dental cleaning, and patient education for self-oral care by a dentist, followed by intensive oral care at least 3 times a day by ICU nurses, and encouragement of self-oral care by floor nurses, tended to decrease anaerobic bacterial amounts in the bronchial fluid and prevented the incidence of postoperative pneumonia.
Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy
Grant number:20592220
2008 - 2010
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
SATO Takuichi, SHIMAUCHI Hidetoshi, TAKAHASHI Nobuhiro, YAMAKI Keiko, MAYANAGI Gen, MATSUYAMA Junko
Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )
Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy The aim of this study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacterial identification, before and after root canal treatment. Samples from infected root canals before and after treatment were collected, followed by anaerobic culture. After incubation, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. The findings of this study suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.
Metabolome analysis of oral biofilm using CE-TOFMS and its application possibility as an oral health indicator
Grant number:19390539
2007 - 2009
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
TAKAHASHI Nobuhiro, HANADA Nobuhiro, KOSEKI Takeyoshi, SENPUKU Hidenobu, SATO Takuichi, SAKAMOTO Mitsuo
Grant amount:\15470000 ( Direct Cost: \11900000 、 Indirect Cost:\3570000 )
Metabolome analysis for the representative plaque bacteria and human oral biofilm using a capillary electrophoresis equipped with a time-of-flight mass spectrometer was established. By this method, a new finding was obtained about the pentose-phosphate pathway and the Kreb's tricarboxylic acid cycle, as well as the Embden-Meyerhof-Parnas pathway. In addition, the metabolome analysis was applicable to a very small amount of oral biofilm. These results suggest that the metabolome data can be used as an indicator of oral health status.
Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.
Grant number:19592193
2007 - 2009
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
YAMAKI Keiko, SHIMAUCHI Hidetoshi, SATO Takuichi
Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )
Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.
Developing Guideline on Oral Health Behavior for Elderly
Grant number:18390605
2006 - 2008
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
SAKASHITA Reiko, SATOU Takuichi, MATSUSHITA Kenji, KAJI Hidesuke, KIM Woesook, ARAI Kanako, MISIGE Ukie, OTSUKA Kumiko, INOUE Masakazu, KUWAHARA Miyoko, NISHIHIRA Tomoko, ABIKO Yuki, HAMADA Misao, YAMAKAWA Tatsuya, OGAWA Hiroyuki, KAMIDE Michiko, TAKIMOTO Naomi
Grant amount:\10450000 ( Direct Cost: \8500000 、 Indirect Cost:\1950000 )
口腔微生物叢の「揺りかご」:舌苔バイオフィルムの生物学
Grant number:17659659
2005 - 2007
System name:科学研究費助成事業 萌芽研究
Research category:萌芽研究
Awarding organization:日本学術振興会
高橋 信博, 小関 健由, 佐藤 拓一, 鷲尾 純平, 今井 奨
Grant amount:\3200000 ( Direct Cost: \3200000 )
本年度は、昨年度に引き続き、口臭原因菌の一つと捉えられる口腔Veillonella属の硫化水素産生活性について検討し、(1)環境因子、とくに口腔内に多く存在する乳酸等の有機酸が硫化水素産生能に与える影響、及び、(2)硫化水素産生に関わる代謝酵素の検索を行った。培養したVeillonella atypica、 V. dispar、 V. parvulaの各標準株(ATCC株)の培養菌にシステインを加えて3時間インキュベートし、産生した硫化水素をメチレンブルー法で定量した。さらにこの反応系に乳酸等の各種有機酸を加え、硫化水素産生活性への影響を検討した。また、培養菌を超音波処理して無細胞抽出液を調整し、システインからの硫化水素産生に関わる酵素活性を検索した。Veillonellaのシステインからの硫化水素産生活性は、乳酸が存在すると著しく増加した。Veillonellaの無細胞抽出液は、システインから硫化水素を産生する酵素活性を示したが、無細胞抽出液に乳酸を添加しても活性の増加は認められなかった。以上のことから、Veillonellaは乳酸の存在下、システインから効率よく硫化水素を産生することが明らかになった。乳酸による促進作用は、酵素の活性化作用ではなく、システインの取込み促進であるものと予測された。口臭源の一つである硫化水素は口腔細菌によって産生されるが、その産生活性はpH(昨年度成果)や有機酸(今年度成果)という口腔環境によって影響を受けるものと考えられ、口腔環境のコントロールによって口臭を抑制し得るものと考えられる。
Comprehensive and quantitative profiling of microflora of human apical periodontitis
Grant number:17591985
2005 - 2007
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
SATO Takuichi, SHIMAUCHI Hidetoshi, TAKAHASHI Nobuhiro, YAMAKI Keiko, MATSUYAMA Junko
Grant amount:\2610000 ( Direct Cost: \2400000 、 Indirect Cost:\210000 )
The purpose of the present study was to profile microflora of root canals before and after root canal therapy, using real-time PCR, PCR-cloning, PCR-sequence analyses based on 16S rRNA genes. Informed consent was obtained from each subject and single-rooted teeth with periapical lesions were investigated. Upon access opening, each dentin sample was collected from the root canal. When the periapical lesion healed clinically through chemo-mechanical cleaning and intracanal medication, the root canal dentin sample was obtained again. The quantification of total bacterial DNA was performed by real-time PCR using universal primers based on 16S rRNA genes. PCR products were cloned and partially sequenced. The partial 16S rRNA gene sequences were then compared with those from the GenBank database using the Blast search program through the website of the NCBI (National Center for Biotechnology Information). The concentrations of bacterial DNA after root canal therapy were lower than those upon access opening. The PCR-cloning and PCR-sequence analyses revealed that Fusobacterium were initially predominant, and that Pseudomonas, Bradyrhizobium and Methylobacterium were predominant after root canal therapy. These results indicated that drastic shifts occurred in microflora of root canals by root canal therapy.
Quantification of oral bacterial flora and application to the risk test of oral diseases
Grant number:17592133
2005 - 2006
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MATSUYAMA Junko, SATO Takuichi, TOMIZAWA Mieko, TAKAHASHI Nobuhiro
Grant amount:\3400000 ( Direct Cost: \3400000 )
The genus Veillonella is small, nonfermentative, anaerobic, gram-negative cocci. Because Veillonella species obtain their energy by fermenting organic acids, e.g., lactate, this metabolism has the potential to remove a potent, dental-caries producing acid. Therefore, the presence of Veillonella in plaque biofilm may reduce the caries-producing potential of plaque biofilm. The aim of this study was to quantify genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of children. After informed consent was obtained, supragingival plaque was obtained from 44 orally-healthy children (1-16 years). Total bacteria and the target genera (Veillonella and Streptococcus) were quantified by real-time PCR using universal and species-specific primers, respectively, and the proportion of each genus was calculated. The proportion of genera Veillonella and Streptococcus were 2.1±4.1% and 19.4±16.7%, respectively. In addition, the proportion of genus Streptococcus was various among individuals, while that of genus Veillonella was comparatively stable in supragingival plaque biofilm, suggesting that genus Veillonella normally inhabits the mouths of both deciduous and permanent dentition.
Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.
Grant number:16390601
2004 - 2006
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
TAKAHASHI Nobuhiro, HATA Shinji, SATO Takuichi, MATSUYAMA Junko, NAKAJO Kazuko, WASHIO Jumpei
Grant amount:\12400000 ( Direct Cost: \12400000 )
Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-& real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.
Establishment of diagnosis of apical periodontitis utilizing the microbiological and immunological profiling of root canals
Grant number:16591905
2004 - 2006
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
YAMAKI Keiko, SHIMAUCHI Hidetoshi, SATO Takuichi, ENDO Hideaki
Grant amount:\3500000 ( Direct Cost: \3500000 )
This study aimed to profile microflora of root canals before and after root canal therapy, utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes. Informed consent was obtained from 5 patients, and 6 single-rooted teeth with periapical lesions were investigated. Upon access opening, dentin sample was collected from the root canal. When the periapical lesion healed clinically through chemo-mechanical cleaning and intracanal medication, the canal dentin was sampled again. The quantification of total bacteria was performed by real-time PCR using universal primers based on 16S rRNA genes. PCR products were cloned and partially sequenced, and bacterial identification was carried out by comparative analysis with the GenBank database. The concentrations of bacterial DNA were 0.36-152.0 (52.7±54.1) ng/mL upon access opening, and lowered to 0.02-36.00 (10.9±16.6) ng/mL after root canal therapy. The cloning-sequence analysis revealed that although Fusobacterium were initially predominant, Pseudomonas, Bradyrhizobium and Methylobacterium prevailed after root canal therapy. These results suggested that changes in bacterial flora brought by root canal therapy were not only in its quantity but also in quality, and that the dramatic shift in its components might also contribute to the healing process.
Combination of genus-specific PCR and RFLP analysis for identification of oral streptococci
2003.6
System name:国際研究集会出席旅費の一部援助
Awarding organization:(公財)井上科学振興財団
佐藤拓一
Authorship:Principal investigator
DNAチップを用いた口腔微生物叢の網羅的定量的プロファイリング
Grant number:15659500
2003 - 2004
System name:科学研究費助成事業 萌芽研究
Research category:萌芽研究
Awarding organization:日本学術振興会
高橋 信博, 小関 健由, 竹原 直道, 佐藤 拓一
Grant amount:\3400000 ( Direct Cost: \3400000 )
本年度は、1.前年度設計した口腔細菌特異的プローブを作成し、DNAチップへの搭載の可否(実用性)を検討し、2.DNAチップへの搭載が可能と考えられた24種のプローブを用いて口腔微生物叢解析用DNAチップを試作し、口腔微生物種特異性、使用法などを検討した。
1.DNAチップ搭載用口腔細菌特異的プローブの作成:
プローブの特異性についてはGeneBankなどの既存のDNAデータベースから、プローブのDNAチップへの搭載の可否についてはDNAチップ作成手順などの実用性から検討し、その結果、24種の口腔微生物に対する特異的プローブが完成した。さらに、この1年間で新たに入手した口腔微生物16S ribosomal RNA遺伝子配列データに基づき、新たなプローブを設計・作成中である。
2.DNAチップ搭載用の24種の口腔微生物特異的プローブを用いた口腔微生物叢解析用DNAチップの試作:
DNAチップ作成共同研究企業にて、24種の口腔微生物特異的プローブを搭載したDNAチップを試作した。各プローブに該当する標準菌株由来DNAを用いて検討したところ、試作したDNAチップは24種の標準菌体由来DNAを特異的に検出することができることがわかった。さらに、DNAチップ上のスポットは肉眼で明瞭に判別でき、市販の可視光用透過型スキャナーで計測・記録することが十分可能であることが分かった。
現在、実際の臨床サンプルを用いて検討中であり、臨床サンプルに含まれる24種の口腔微生物の従来法による検出(通常のnested PCR法での検出)を終え、DNAチップによる検出との照合を進めつつある。
高齢者歯髄の免疫防御機能に関する研究
2002.4 - 2003.3
System name:第29回調査研究助成
Awarding organization:(公財)大和証券ヘルス財団
大島勇人,佐藤拓一
Authorship:Coinvestigator(s)
Microbiological study on oral bacterial floral shifts in children
Grant number:14571944
2002 - 2004
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MATSUYAMA Junko, TOMIZAWA Mieko, SATO Takuichi, TAKAHASHI Nobuhiro, NODA Tadashi
Grant amount:\3400000 ( Direct Cost: \3400000 )
Mutans streptococci have been implicated as cariogenic bacteria in the oral cavity. It has been reported that the diversity of detection frequencies of mutans streptococci from human dental plaque, and these diversity may be due to the differences of subject age and/or target genes tested. In this study, detection of mutans streptococci from human dental plaque were performed with polymerase chain reaction(PCR) using primers base upon 16S ribosomal RNA, glucosyltransferase(gft) and dextranase(dex) genes, and their detection frequencies were compared. Detection frequencies of mutans streptococci by PCR with primers based upon the 16S rRNA genes were higher than those based upon the gtf and dex genes from human dental plaque of children. Utilizing PCR with primers based upon the 16S rRNA genes, S.mutans were detected from all of the samples tested in this study. On the other hand, the detection frequencies of S.sobrinus were lower (10%) than those of S.mutans, and S.sobrinus were likely detected in the subjects of deciduous attained-dentition and mixed-dentition stage. The 16S rRNA gene-based nested PCR method is a rapid and sensitive method for the detection of mutans streptococci, and may also be suitable for carrying out large-scale studies on the cariogenicity of mutans streptococci. Furthermore, this method can potentially detect not only mutans streptococci but also other oral bacterial species, such as periodontopathic bacteria, by substituting specific primers for each oral bacterial species during the second PCR.
Changes of Serum Lipid Levels induced by Periodontal Infection in Rats
Grant number:14571809
2002 - 2004
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
ITAGAKI Yumi, SHIMAUCHI Hidetoshi, SATO Takuichi
Grant amount:\3400000 ( Direct Cost: \3400000 )
In recent years, some evidence has been presented that serum antibody titer against Porphyromonas gingzvalis(Pg), cholesterol, triglyceride and blood glucose levels were significantly higher in periodontally diseased patients than in healthy subjects. Thus, it is suggested that periodontal disease could influence the metabolism of lipids and glucose. This study examined the effects of experimentally induced periodontitis on the serum level of triglyceride, glucose, cytokine(TNF-α) and C-reactive protein(CRP) in rats.
Twenty-nine female Wistar rats were divided into the following 3 groups : Pg-infected group(n=9), Pg-non infected group(n=8) and control group(n=12). Experimental periodontitis was induced by applying a small elastic ring around the mandibular first molar(M1) in Pg-infected and non infected groups. After one week, Pg soaked or sterile silk sutures were inserted into periodontal pockets and fixed by elastic rings around M1 of rats in the Pg-infected or non infected group, respectively. Silk sutures and elastic rings were changed after 2 weeks. Two more weeks after changing the sutures and elastic rings, rats were sacrificed by drawing blood from the hearts and removed mandibular bones. Rats of control group were not treated and maintained for 5 weeks.
Significant alveolar bone loss was found in the Pg-infected (2.20±0.14mm) and non infected (2.14±0.13mm) groups compared with that in the control group (1.36±0.11mm). However, there was no significant difference between Pg-infected and non infected groups. The blood glucose level was significantly higher in the Pg-infected group(143.1±6.1mg/dl) than that in the Pg-non infected (132.3±4.8mg/dl) and control groups (132.7±9.4mg/dnl). With regard to triglyceride, there was no significant difference in the three groups because of large individual differences. TNF-α levels of all groups were below the detectable level(<0.7pg/ml). There was no significant difference in the CRP level.
It was considered that Pg-infection of periodontium could raise the blood glucose level. However, TNF-α, which has been considered involved in the increased glucose level, was not detected in peripheral blood. Further studies are needed to determine the mechanisms underlying the association between Pginfection of the periodontium and an increase in the glucose level.
Possible role of immunocompetent cells and the expression of Hsp25 in the process of pulpal regeneration
Grant number:14571727
2002 - 2003
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
OHSHIMA Hayato, SATO Takuichi, FUJII Noritaka, NAKAKURA Kuniko, MAEDA Takeyasu
Grant amount:\3000000 ( Direct Cost: \3000000 )
The purpose of the present study was to clarify the relationship between the chronological changes of immunocompetent cells and the expression of heat shock protein(Hsp) 25 in the process of pulpal regeneration after tooth injury in rat molars by immunocytochemistry for Hsp 25 and class II major histocompatibility complex(MHC) antigen. An intense Hsp 25-immunoreactivity was found in the differentiated odontoblasts. Both cavity preparation and tooth replantation caused the degeneration of the odontoblast layer to result in the loss of Hsp 25-immunoreactions in the suffered dental pulp at the early stages after tooth injury. Numerous class II MHC-positive cells appeared along the pulp-dentin border and extend their cell processes into the dentinal tubules at 12-24 hours after cavity preparation and 3 days after tooth replantation. Newly differentiated odontoblast-like cells with Hsp 25-immunoreactivity were arranged at the pulp-dentin border, and the class II MHC-positive cells retreated towards the subodontoblastic layer by postoperative 3-5 days after tooth injury. Thus, the common cellular events occur during pulpal regeneration following two different experimental injuries. These findings indicate that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the degeneration/regeneration process of odontoblasts and that the temporal appearance of the class II MHC-positive cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. In the case of laser ablation, on the other hand, distinct abscess formation consisting of polymorphonuclear leukocytes was found in the dental pulp by 3-5 days postoperation. The penetration of masses of oral bacteria was recognizable in the dentinal tubules beneath the prepared cavity. These findings indicate that cavity preparation by laser ablation induces remarkable inflammation by continuous bacterial infections via dentinal tubules.
16S rRNA genes PCR-RFLPによる口腔細菌叢解析システムの構築
Grant number:14771000
2002 - 2003
System name:科学研究費助成事業 若手研究(B)
Research category:若手研究(B)
Awarding organization:日本学術振興会
佐藤 拓一
Grant amount:\2800000 ( Direct Cost: \2800000 )
口腔領域の細菌叢の解析手法は、古典的な培養法及び生化学的性状検査法から、PCR法を利用した分子生物学的解析法にシフトしつつある。そこで本研究では16S rRNA genes PCR-RFLP法を齲蝕や歯周病に関連する口腔細菌の同定に応用し、細菌叢解析における有効性について検討した。
口腔のグラム陽性菌(Actinomyces属、Streptococcus属など)及びグラム陰性菌(Porphyromonas属、Prevotella属、Fusobacterium属など)の各標準株と臨床分離株を用いた。16S rRNA genesのユニバーサルプライマーを用いてPCRを行ない、得られたPCR産物を制限酵素AluI、Hae IIあるいはHpaIIで処理した。制限酵素断片のアガロース電気泳動像を記録し、各菌種のパターンを、GenBankのデータベースを基に計算した結果と比較した。その結果、各制限酵素処理後の泳動像は、各種の口腔細菌に特異的なパターンを示し、また得られたパターンはデータベースから計算した結果と一致した。以上のことから16S rRNA genes PCR-RFLP法は、口腔細菌の同定に応用可能であり、さらに迅速・簡便であることが判明した。さらに、16S rRNA genes PCR-RFLP法(グループ分け)とシークエンス解析を組み合わせることにより、口腔細菌種同定のスタンダードな方法となりうることが示唆された。
APPLICATION OF SUGAR ALCOHOLS COCKTAILS TO HUMAN CARIES PREVENTION
Grant number:14370687
2002 - 2003
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
TAKAHASHI Nobuhiro, HATA Shinji, NODA Tadashi, MAYANAGI Hideaki, SATO Takuichi, MATSUYAMA Junko
Grant amount:\6800000 ( Direct Cost: \6800000 )
Xylitol inhibits the glycolysis and growth of Streptococcus mutans. We studied the inhibitory effect of xylitol on the acid production of S.mutans at several pH levels under the strictly anaerobic conditions found in the deep layer of dental plaque. Xylitol inhibited the rate of acid production from glucose and changed the profile of acidic end products to formate-acetate dominance, with a decrease in the intracellular level of fructose 1,6-bisphosphate and an intracellular accumulation of xylitol 5-phosphate (X5P). These results were notable at pH 5.5-7.0, but were not evident at pH 5.0. Since the activity of phosphoenolpyruvate phosphotransferase for xylitol was greater at higher pH, it is suggested that xylitol could be incorporated more efficiently at higher pH and that the resultant accumulation of X5P could inhibit the glycolysis of S.mutans more effectively.
In addtion, xylitol retarded the growth of mutans streptococci in the presence of glucose (G), galactose (Gal), maltose (M), lactose (L) or sucrose (5) as an energy source, though the inhibition of growth on fructose (Fr) was small. Xylitol inhibited acid production by washed cells of S.mutans from G,Gal,M,L or 5 (12-83% inhibition). S.mutans accumulated xylitol 5-phosphate (X5P) intracellularly through activity of the phosphoenolpyruvate-xylitol phosphotransferase system (PEP-xylitol PTS) when they fermented these sugars in the presence of xylitol. However, in the presence of Fr, no inhibition of acid production was observed. In addition, the amounts of X5P during the fermentationof Fr were smaller than those of other sugars in spite of the presence of PEP-xylitol PTS activity. These results suggest that along with the intracellular accumulation of XSP, xylitol decreases the growth and acid production of mutans streptococci in the presence of various dietary sugars except Fr.
口腔領域の慢性感染症、特に下顎骨骨髄炎及び感染性歯根嚢砲に関する研究
2001.4 - 2002.3
System name:第28回調査研究助成
Awarding organization:(公財)大和証券ヘルス財団
佐藤拓一, 高橋信博
Authorship:Principal investigator
MICROBIOLOGICAL STUDY ON DENTAL CARIES PREVENTION USING SUGAR SUBSTITUTES
Grant number:13470446
2001 - 2003
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
MAYANAGI Hideaki, MATSUYAMA Junko, HATA Shinji, TAKAHASHI Nobuhiro, SATO Takuichi
Grant amount:\8400000 ( Direct Cost: \8400000 )
Xylitol inhibits the glycolysis and growth of Streptococcus mutans (S. mutans). In this study, we studied the inhibitory effect of xylitol on the acid production of S. mutans at several pH levels under the strictly anaerobic conditions found in the deep layer of dental plaque. Xylitol inhibited the rate of acid production from glucose and changed the profile of acidic end products to formats-acetate dominance, with a decrease in the intracellular level of fructose 1,6-bisphosphate and an intracellular accumulation of xylitol 5-phosphate (X5P). These results were notable at pH 5.5-7.0,but were not evident at pH 5.0. Since the activity of phosphoenolpyruvate phosphotransferase for xylitol was greater at higher pH, it is suggested that xylitol could be incorporated more efficiently at higher pH and that the resultant accumulation of X5P could inhibit the glycolysis of S. mutans more effectively.
口腔細菌の菌種名同定のためのPCR-RFLP図鑑の作成
Grant number:13922019
2001
System name:科学研究費助成事業 奨励研究(B)
Research category:奨励研究(B)
Awarding organization:日本学術振興会
佐藤 拓一
Grant amount:\240000 ( Direct Cost: \240000 )
Study on the role of heat shock protein 27 in the process of development and regeneration of dental pulp
Grant number:12671765
2000 - 2001
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
OHSHIMA Hayato, SATO Takuichi, KAWANO Yoshiro, MAEDA Takeyasu
Grant amount:\3900000 ( Direct Cost: \3900000 )
The present study aims to clarify the functional significance of heat shock protein (Hsp) 25/27 during tooth development and pulpal regeneration. The present study demonstrated that Hsp 25/27 was expressed exclusively in fully-differentiated odontoblasts during tooth development. For the observation of pulp regeneration, Wistar rats, 4-week-old and 100-day-old, were used for tooth replantation and cavity preparation, respectively. Cavity preparation caused an edematous reaction between the injured odontoblasts and predentin, but the immunoreaction for Hsp 25/27 remained in the injured odontoblasts. Subsequently, Hsp 25/27-immunoreactivity disappeared in the degenerated odontoblast layer after 12 hours. On postoperative 3 days, newly differentiated odontoblasts replaced the degenerated odontoblasts, and became to exhibit the immunoreaction for Hsp 25/27. Tooth replantation also caused the disappearance of Hsp 25/27-immunopositive cells at the initial stages. On postoperative 5days, plump cells with clear nucleoli at the dentin-pulp border became to show Hsp 25/27-immureactivity. These findings indicate that newly differentiated odontoblasts acquire the immunoreaction for Hsp 25/27 in the regenerated pulpal tissue after both cavity preparation and tooth replantation. There was no Hsp 25/27-immunopositive cell along the pulp-dentin border in the case of bone-like tissue formation in the pulp space following tooth replatation. Thus, the alignment of Hsp 25/27-immunopostive cells along the pulp-dentin border is suggestive of the decisive factor to induce the reparative dentin formation after tooth replantation.
STUDY ON METABOLIC SYSTEMS FOR PEPTIDES AND AMINO ACIDS OF PERIODONTAL PATHOGENS, PORPHYROMONAS AND PREVOTELLA.
Grant number:11470386
1999 - 2001
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Research category:Grant-in-Aid for Scientific Research (B)
Awarding organization:Japan Society for the Promotion of Science
TAKAHASHI Nobuhiro, SATO Takuichi, IWAMI Yoshimichi
Grant amount:\10400000 ( Direct Cost: \10400000 )
1. Peptides and amino acids as sources for the growth and the energy production of Porphyromonas gingivalis (Pg) and Prevotella intermedia(Pi)
Periodontal pathogens, P. gingivalis and P. intermedia utilized peptides, especially peptides containing aspartate and/or glutamate, as the sources of their growth and energy production. In addition, P. gingivalis preferentially utilized aspartylaspartate and glutamylglutamate, while P. intermedia utilized aspartate and aspartylaspartate. The observation that both bacteria had no dipeptidase activity on their cell surfaces indicates that they incorporate dipeptides through a dipeptide-specific transport system. These findings suggest the existence of segregation or competition between P. gingivalis and P. intermedia for their nutrients.
2. Metabolic pathways for peptides and amino acids of P. gingivalis and P. intermedia
Metabolic system for peptides and amino acids of P. gingivalis and P. intermedia consists of the following steps: (l) peptide uptake (2) hydrolysis of peptide into amino acid (3) deamination of amino acid into 2-oxoacid (4) oxidation of 2-oxoacid into acyl-CoA and (5) production of short-chain fatty acid. 2-oxoacid oxidoreductases, which catalyze the reaction (4), utilized an artificial electron acceptor, methylviologen. This property is similar to that of enzymes found in archea.
3. Molecular biological method for identification of periodontal pathogens
From the ecological point of view as described 1, a molecular biological method for bacterial identification, was developed to know the distribution in dental plaque of periodontal pathogens, P. gingivalis and P. intermedia. A new method, PCR-RFLP based on bacterial 16S ribosomal RNA gene, was found to be not only simple and fast but also more reliable than the conventional method such as biological and morphological tests.
Joint study on oral asaccharolytic Eubacterium species.
Grant number:06044082
1994 - 1995
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for international Scientific Research
Research category:Grant-in-Aid for international Scientific Research
Awarding organization:Japan Society for the Promotion of Science
HOSHINO Etsuro, GREENMAN John, WEIGHTMAN Andrew j., ELWORTHY Alison j., HIOM Sarah j., WADE William g., NAKAZAWA Futoshi, SATO Takuichi
Grant amount:\6200000 ( Direct Cost: \6200000 )
Asaccharolytic Eubacterium species are strictly anaerobic, gram-positive rods and are frequently isolated and predominant bacterial species from different oral samples. The slow growth and oxygen sensitivity of the oral asaccharolytic Eubacterium species has hindered the determination of their significance in various oral infectious diseases. By adopting efficient anaerobic isolation procedures, we isolated several unclassified strains of Eubacterium that were distinct from the established Eubacterium species namely, E.brachy, E.lentum, E.nodatum and E.timidum. A polyphasic approach was used to examine the taxonomic positions of these organisms. These un-named strains were inert in conventional biochemical tests, however, the results of SDS-PAGE protein profile analysis and aminopeptidase activty (Rapid ID 32A) revealed that these strains were quite distinct compared from the established asaccharolytic species and assigned to four groups. Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity. The strains in the fourth group produced a trace (if any) of phenylacetate. The continuos development and improvement of anaerobic incubation and identification techniques resulted in the isolation of slow growing and culture-difficult asaccharolytic species of Eubacterium. Further studies are in progress to provide information on the roles of the unclassified strains and other Eubacterium species in oral disease.
小児の口腔内慢性感染病巣細菌の全身への免疫学的影響-その細菌学的・免疫学的アプロ-チ-
1993.4 - 1996.3
System name:科学研究費助成事業 特別研究員奨励費
Research category:特別研究員奨励費
Awarding organization:日本学術振興会
佐藤拓一
Authorship:Principal investigator
東南アジアにおける歯周疾患に関する国際共同研究-特に日本、バングラデシュ、フィリピンの比較実態調査と文化的背景分析-
1993.4 - 1994.3
System name:国際共同研究
Awarding organization:(公財)ファイザ-ヘルスリサ-チ振興財団
星野悦郎, 佐藤拓一, M.A. Kalam Joarder
Authorship:Coinvestigator(s)
東南アジアにおける歯周疾患に関する国際共同研究-特に日本、バングラデシュ、フィリピンの比較実態調査-
1993.4 - 1994.3
System name:APIC国際協力学術奨励金
Awarding organization:(一財)国際協力推進協会
佐藤拓一, 星野悦郎, M.A. Kalam Joarde
Authorship:Principal investigator
新しい乳歯齲蝕・感染根管治療法の確立-病巣無菌化療法-
1993.4 - 1994.3
System name:科学研究費助成事業 試験研究(B)
Research category:試験研究(B)(2)
Awarding organization:日本学術振興会
野田忠, 星野悦郎, 子田晃一, 鈴木 誠, 吉羽邦彦, 大島勇人, 佐藤拓一
Authorship:Coinvestigator(s)
Basic research for evaluating caroigenic potential of foods and sugar substitutes.
Grant number:04304045
1992.4 - 1994.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for Co-operative Research (A)
Research category:Grant-in-Aid for Co-operative Research (A)
Awarding organization:Japan Society for the Promotion of Science
YAMADA Tadashi, KANEHIRA Takashi, IIJIMA Yoichi, MATSUKUBO Takashi, NISHIZAWA Toshiki, HOSHINO Etsuro
Grant amount:\12000000 ( Direct Cost: \12000000 )
We have investigated for 3 years how we should evaluate cariogenicity of foods and materials for good processing. We reached the conclusions as follows :
1.It is important and essential to distinguish the evaluation of cariogenicity of foods from the evaluation of the materials for food processing.
2.It is reasonable to accept the definition at the San Antonio Consensus Conference in 1986 about the negative and the positive controls. These are sucrose as the positive and sorbitol as the negative control.
3.If a food does not drop plaque pH no less than 5.7by indwelling electrode method, this food could be concluded as noncariogenic. Non-acidogenic food should be noncariogenic.
4.On the other hand all the foods drop the pH less than 5.7 should not be considered cariogenic. This kind of misleading should be avoided.
5.If a food drops plaque pH less than 5.0, this food does not seem worth evaluating its cariogenicity by other methods. This food may have little chance to be evaluated noncariogenic, or even low cariogenic.
6.If a food drops plaque pH between 5.7 an 5.0 or 5.2, this food seems to be worth testing for its low cariogenicity by animal experiments and intraoral cariogenicity tests.
7.A test to find out the microorganisms in dental plaque to be able to ferment a sugar substitute gives us a useful information to find out if microorganisms in dental plaque have a possibility to adapt to this sugar substitute.
8.There are many difficulties to use animal experiments for evaluating cariogenicity of a food for human.
Bacteriological and Immunological study on endodontic lesions of human deciduous teeth
Grant number:04807150
1992.4 - 1994.3
System name:Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (C)
Research category:Grant-in-Aid for General Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
NODA Tadashi, SATO Takuichi, OHSHIMA Kuniko, ISHIKURA Yuuka, HOSHINO Etsuro
Authorship:Coinvestigator(s)
Grant amount:\1800000 ( Direct Cost: \1800000 )
The purpose of this study was to investigate the bacterial composition of necrotic pulps of human deciduous teeth by sampling the split surfaces of freshly extracted teeth and culturing the bacteria present with good anaerobic isolation techniques. Significantly more bacteria were recovered after the incubation in an anaerobic chamber than after aerobic incubation in air with 30 per cent CO_2. Of 276 bacterial isolates, 251 (91 per cent) were obligate anaerobes. These findings suggest that the environment of necrotic pulps in human deciduous teeth is anaerobic and thus favors the growth of anaerobes. Among the 251 obligate anaerobes isolated, strains belonging to the genera Peptostreptococcus (25 per cent), Propionibacterium (19 per cent), Eubacterium (17 per cent) and Fusobacterium (13 per cent) were major parts of the bacterial flora of the lesions of human deciduous teeth. Bifidobacterium (2 per cent), Lactobacillus (1 per cent), Actinomyces (1 per cent) and Veillonella (0.7 per cent) were minor parts of the flora. The microflora of necrotic pulps of human deciduous teeth is in some respects similar to that reported for the deep layrs of dentinal lesions of adults.
実験技術トレーニングコース
生体分子の科学IおよびII
博士論文特別研修担当
卒業研究
病態化学分析学実習Ⅱ
医療英語(検査)
分析系検査管理論
医学検査機器概論
病態化学分析学実習Ⅰ
病態化学分析学Ⅱ
病態化学分析学Ⅰ
保健学特別研究(検査技術科学)
医学検査管理総論
医療安全管理学
臨床検査実習
生体システム機能検査学特講演習
生体システム機能検査学特講
臨床検査管理概論
スタディスキルズ (検査)
生体情報解析学実習
生体情報解析学特論
入門医療英語
臨床検査実習I
臨床検査実習II
保健学総合
リサーチ・メソッズ・アドバンスト
保健学特定研究(検査技術科学)
生活習慣と健康
保健学特別研究(検査技術科学)
医療英語ベーシック(検査)
生体システム機能検査科学特講
生体システム機能検査科学特講演習
保健学特別研究(検査技術科学)
医学検査管理総論
医療安全管理学
医療英語(検査)
卒業研究
臨床検査実習
分析系検査管理論
病態化学分析学実習Ⅱ
医学検査機器概論
スタディスキルズ (検査)
生体情報解析学特論
病態化学分析学実習Ⅰ
生体情報解析学実習
病態化学分析学Ⅰ
病態化学分析学Ⅱ
入門医療英語
生体システム機能検査学特講
臨床検査管理概論
生体システム機能検査学特講演習
保健学科のタイムトラベル ~歩みを知り、今を体験する~(展示・出展)【飲みかけのペットボトル内の細菌を測ってみよう!】
Role(s): Advisor, Planner
新潟大学キャンパスまるごとミュージアム NEXUS 2026.11
飲みかけのペットボトル内の細菌を測ってみよう!
Role(s): Planner, Organizing member, Demonstrator
【新大WeeK・特別企画】開講!新潟大学@古町ルフル 2026.11
ワンランク上を目指す研究室 ~佐藤拓一研究室のとりくみ~(佐藤拓一,涌井杏奈,河内美帆)
Role(s): Lecturer
大学女性協会新潟支部2026年度総会(新潟) 2026.4
Role(s): Lecturer, Demonstrator
【新大WeeK・特別企画】開講!新潟大学@古町ルフル 2025.10
女性の博士後期課程進学に向けて《動画》(加藤優希,佐藤遥菜,佐藤 彩)
Role(s): Advisor
保健学研究科へ進学する意思決定を後押しできる広報コンテンツの作成 2024.6
保健学における次世代を担う女性研究者育成の取り組みについて(涌井杏奈,佐藤拓一)
Role(s): Appearance
新潟大学大学院保健学研究科Web site(動画) 2023.8
大学の研究室で身近な微生物について一緒に調べてみよう!(不織布マスクやスマホ画面の細菌に関する実験)
Role(s): Lecturer, Advisor, Planner, Demonstrator
新潟大学大学院保健学研究科臨床化学研究室 2023.7
(新潟県教育委員会)高校生アカデミック・インターンシップ研修「大学の研究室で身近な細菌について一緒に調べてみよう!」
Role(s): Lecturer, Advisor, Planner, Demonstrator
新潟大学医学部保健学科 2023.7 - 2024.8
N-Step新潟ジャンピングステージ
Role(s): Advisor
新潟大学 2023.4 - 2026.3
(新潟県長岡市内)小学5年生PTA活動(大学訪問)「ペットボトルのお茶をラッパ飲みすると…」
Role(s): Lecturer, Demonstrator
新潟大学附属長岡小学校 2022.11
新潟妙高会(第32回総会)講演の【企画】「新潟大学の将来ビジョン ー未来のライフ・イノベーションのフロントランナーになるー」
Role(s): Planner
ホテル・イタリア軒 2022.11
新潟ジュニアドクター育成塾およびサイエンスカンファレンスでの研究発表「使用済みマスクに生息する細菌の分析:細菌の量からマスクの再利用の可能性を考える」
Role(s): Advisor
新潟大学 2022.7 - 2023.3
(新潟市内)私立高等学校(進学セミナー:出張講義)「食品に関わる微生物学:飲み残したペットボトルの口の部分および飲料物中で増殖する微生物に関する最新知見」
Role(s): Lecturer
新潟明訓高等学校 2021.6
新潟ジュニアドクター育成塾およびサイエンスカンファレンスでの研究発表「飲みかけのペットボトル飲料(お茶、スポーツ飲料、コーラ、オレンジジュース)に生息する細菌について」
Role(s): Advisor
新潟大学 2020.8 - 2020.12
(新潟県)青少年のための科学の祭典「飲みかけのペットボトル内の細菌を測ってみよう!:お茶や清涼飲料水について」
Role(s): Lecturer, Demonstrator
アオーレ長岡 2020.2
(新潟市内)県立高等学校(大学講義体験)「食品に関わる微生物学:飲みかけのペットボトルのお茶や清涼飲料水に生息する細菌について」
Role(s): Lecturer
新潟南高等学校 2019.12
(新潟市内)小学6年生 総合的な学習の時間(夢先生に学ぼう)「大学教員(大学院)の紹介:新潟大学医学部保健学科」
Role(s): Lecturer
女池小学校 2019.11
新潟妙高会(第31回総会)講演の【企画】「顕微鏡に魅せられて」
Role(s): Planner
ホテル・イタリア軒 2019.11
(新潟市内)小学校全校遠足(なかよし班の友情で楽47ツアー)「ペットボトルのお茶をラッパ飲みすると・・・(微生物のはなし)」
Role(s): Lecturer
新潟大学総合教育研究棟(五十嵐キャンパス) 2019.9
(新潟県教育委員会)高校生アカデミック・インターンシップ研修「飲みかけのペットボトルのお茶や清涼飲料水に生息する細菌について」
Role(s): Lecturer, Advisor, Planner, Demonstrator
新潟大学医学部保健学科 2019.7 - 2019.12
新潟妙高会(第30回総会)講演の【企画】「見えない障害:難聴」
Role(s): Planner
ホテル・イタリア軒 2018.11
新潟妙高会(第29回総会)講演の【企画】「歯と健康」
Role(s): Presenter, Planner
ホテル・イタリア軒 2017.11
大学院生・河内美帆さんが,「学術研究活動において,特に顕著な成果を挙げ,学界または社会的に高い評価を受けた者」として学生表彰されました
Web site@新潟大学大学院保健学研究科 2026.3
大学院博士後期課程の河内美帆さん(検査技術科学分野)が、大学女性協会新潟支部「奨学賞」を受賞しました
Web site@新潟大学大学院保健学研究科 2025.12
大学院博士前期課程の関口未来さん(検査技術科学分野)が、院生会第5回研究発表会にて最優秀賞を受賞しました
Web site@新潟大学大学院保健学研究科 2025.11
第31回コカ・コーラ環境教育賞・最終選考参加団体紹介:新潟大学大学院保健学研究科・臨床化学研究室土曜ゼミ (新潟市) Internet
(公益財団法人)コカ・コーラ教育・環境財団 2025.10
保健学科4年の成瀬悠香さんが、第74回日本口腔衛生学会学術大会にて学生ポスター発表奨励賞を受賞しました
Web site@新潟大学大学院保健学研究科 2025.6
スマートフォン画面や不織布マスクの細菌について Newspaper, magazine
日本経済新聞 土曜朝刊「NIKKEIプラス1」 2025.5
保健学研究科大学院生・河内美帆さんが、国立大学臨床検査学系博士後期課程・最優秀賞を受賞しました
Web site@新潟大学大学院保健学研究科 2025.4
ゼミ研「ワンランク上を目指す研究室:医学部保健学科 佐藤拓一研究室」 Promotional material
新大広報 WEB版 2025.3
大学院生の今井真奈美さんが、院生会第4回研究発表会にて優秀賞を受賞しました!
Web site@新潟大学大学院保健学研究科 2024.12
検査技術科学分野の大学院生が、大学女性協会新潟支部「奨学賞」を受賞
Web site@新潟大学大学院保健学研究科 2024.12
検査技術科学分野の佐藤拓一教授らの研究論文が日本分析化学会関東支部新潟地区部会で表彰されました
Web site@新潟大学大学院保健学研究科 2024.11
保健学科4年の加藤優希さんが、第77回伝統食品研究会にて学生研究奨励賞を受賞しました
Web site@新潟大学大学院保健学研究科 2024.10
2023年3月に大学院を修了した涌井杏奈さんが、日本臨床化学会のYoung Investigator Award(若手研究者賞)を受賞しました
Web site@新潟大学大学院保健学研究科 2023.11
夏場、口をつけたペットボトルは危険は誤解? 麦茶では細菌数が増加も、スポーツ飲料は減少する理由 Newspaper, magazine
朝日新聞出版 AERAdot. 編集部 2023.8
この3月に大学院を修了した涌井杏奈さんが、国立大学臨床検査学系博士後期課程優秀賞を受賞しました
Web site@新潟大学大学院保健学研究科 2023.5
検査技術科学分野の大学院生・涌井杏奈さんが、大学女性協会新潟支部「奨学賞」を受賞しました
Web site@新潟大学大学院保健学研究科 2022.12
保健学科2年の加藤優希さんが、新潟大学院生会2022年度第2回研究発表会にて最優秀賞を受賞しました
Web site@新潟大学大学院保健学研究科 2022.12
大学院生・涌井杏奈さんが、「学術研究活動において,特に顕著な成果を挙げ,学界または社会的に高い評価を受けた者」として学生表彰されました
Web site@新潟大学大学院保健学研究科 2022.3
保健学科4年の高橋七瀬さんが、大学女性協会新潟支部「奨学賞」を受賞しました
Web site@新潟大学大学院保健学研究科 2021.12
大学院生・河内美帆さんと学部4年生の高橋七瀬さんが、第61回日本臨床化学会にて学術奨励賞を受賞しました
Web site@新潟大学大学院保健学研究科 2021.11
検査技術科学分野の大学院生・涌井杏奈さんが、第60回日本臨床化学会にて奨励賞を受賞しました
Web site@新潟大学大学院保健学研究科 2020.11
亜硝酸塩できる過程解明へ(関与の常在細菌判明)むし歯や循環器疾患の予防に効果 Newspaper, magazine
新潟日報 2020.11
口腔内細菌の亜硝酸塩産生活性は舌苔より歯垢で高く、個人差が大きい-新潟大ほか Internet
医療NEWS QLifePro 2020.10
科学技術振興機構『さくらサイエンスプラン』友情と感激=特別シリーズ=第225回 新潟大学の活動報告 Newspaper, magazine
文教ニュース 第2613号 p. 46-47 2020.8
スリランカの若手大学教員を招き保健学最先端研究の体験型演習を実施し、日本とスリランカの将来構想を共に考えた Internet
2019年度活動レポート(一般公募コース)第273号(日本-アジア青少年サイエンス交流事業・さくらサイエンスプラン) 2020.3
「科学って面白い!」を伝えた2日間:新潟大学 青少年のための科学の祭典(医学部 保健学科)飲みかけのペットボトル内の細菌を測ってみよう! Newspaper, magazine
朝日新聞 別刷り特集 4面 2020.3
新潟)飲み物にこんなに細菌!?長岡で科学の祭典 Newspaper, magazine
朝日新聞 2020.2
検査技術科学分野の大学院生・涌井杏奈さんらが、第59回日本臨床化学会にてトラベルアワードを受賞しました
Web site@新潟大学大学院保健学研究科 2019.10
The Update Symposium in The 68th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc.
歯科基礎医学会 (名古屋) 2026.9
The Update Symposium in The 67th Annual Meeting of the JAOB
Role(s): Planning, management, etc.
歯科基礎医学会 (北九州) 2025.9
第19回 日本臨床検査学教育学会学術大会(岡山)大学院生セッション
Role(s): Panel moderator, session chair, etc., Review, evaluation
日本臨床検査学教育協議会 2025.8
第65回 日本臨床化学会(名古屋)「学生シンポジウム」シンポジスト選出審査
Role(s): Review, evaluation
2025.7
第98回日本細菌学会(金沢)ワークショップ9「臨床検査学分野の細菌学研究」
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
日本細菌学会 2025.5
The Update Symposium in The 66th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc.
歯科基礎医学会 ( 長崎 ) 2024.11
第18回 日本臨床検査学教育学会学術大会(新潟)実行委員長
Role(s): Planning, management, etc.
新潟大学医学部保健学科検査技術科学専攻 2024.8
第63回日本臨床化学会年次学術集会(東京)「学生シンポジウム」およびシンポジスト選出審査
Role(s): Review, evaluation
2023.10
The Update Symposium in The 65th Annual Meeting of the JAOB
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 東京 ) 2023.9
第17回 日本臨床検査学教育学会学術大会(奈良)学部生セッション
Role(s): Review, evaluation
日本臨床検査学教育協議会 2023.8
第12回口腔保健用機能性食品研究会(新潟)大会長
Role(s): Planning, management, etc.
新潟大学大学院保健学研究科臨床化学研究室 2023.2
The Update Symposium in The 64th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc.
歯科基礎医学会 ( 徳島→台風にて録画+ハイブリッド開催 ) 2022.9
第16回 日本臨床検査学教育学会学術大会(埼玉)学部生セッション
Role(s): Review, evaluation
日本臨床検査学教育協議会 2022.8
The Update Symposium in The 63rd Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 横須賀→コロナ禍にてオンライン開催 ) 2021.10
The Update Symposium on The 62nd Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc.
2020.9
The Update Symposium on The 61st Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
2019.10
第59回日本臨床化学会年次学術集会(仙台)学生シンポジウム
Role(s): Panel moderator, session chair, etc.
日本臨床化学会 2019.9
The 3rd GSH International Symposium, “Health Sciences in Sri Lanka” International contribution
Role(s): Panel moderator, session chair, etc.
( 新潟大学 大学院保健学研究科 ) 2018.12
第13回 日本臨床検査学教育学会学術大会(札幌)学部生セッション
Role(s): Review, evaluation
日本臨床検査学教育協議会 2018.8
The 2nd GSH International Symposium, “Technology Supporting Medical Care” International contribution
Role(s): Panel moderator, session chair, etc.
( 新潟大学 大学院保健学研究科 ) 2018.2
第57回日本臨床化学会年次学術集会(札幌)学生シンポジウム
Role(s): Panel moderator, session chair, etc.
日本臨床化学会 2017.10
The Update Symposium on The 59th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 塩尻 ) 2017.9
第12回 日本臨床検査学教育学会学術大会(埼玉)学部生セッション
Role(s): Review, evaluation
日本臨床検査学教育協議会 2017.8
第9回NOA-METS講演会
Role(s): Panel moderator, session chair, etc.
( 新潟大学 大学院保健学研究科 ) 2017.2
The Main Symposium on The 58th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 札幌 ) 2016.8
The Satellite Symposium on The 57th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 新潟 ) 2015.9
The Satellite Symposium on The 56th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 福岡 ) 2014.9
The Satellite Symposium on The 55th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 岡山 ) 2013.9
The Satellite Symposium on The 54th Annual Meeting of the JAOB International contribution
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 郡山 ) 2012.9
第51回歯科基礎医学会学術大会(新潟) 一般演題:微生物2
Role(s): Panel moderator, session chair, etc.
歯科基礎医学会 2009.9
第48回歯科基礎医学会学術大会 サテライトシンポジウム「口腔生態系研究の新展開」
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 鶴見 ) 2006.9
第47回歯科基礎医学会学術大会 サテライトシンポジウム「口腔微生物叢研究の新展開」
Role(s): Planning, management, etc., Panel moderator, session chair, etc.
歯科基礎医学会 ( 仙台 ) 2005.9