Updated on 2025/09/01

写真a

 
MIURA Shiori
 
Organization
. . Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
External link

Degree

  • 博士 (農学) ( 2017.3   岐阜大学 )

Research Interests

  • Biosafety

  • Biorisk management

  • 移植学

  • 実験動物学

  • 遺伝子発現解析

Research Areas

  • Life Science / Laboratory animal science

  • Life Science / Molecular biology

Research History (researchmap)

  • Niigata University   Institute for Research Administration   Assistant professor

    2024.1

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  • Niigata University   Institute for Research Promotion   Visiting researcher

    2022.4 - 2024.12

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  • Niigata University   Institute for Research Administration   Specially Appointed Assistant Professor

    2020.7 - 2022.3

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  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2017.10 - 2020.6

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  • Kagoshima University   Center for Advanced Biomedical Science and Swine Research

    2012 - 2015

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  • 株式会社しか屋   バイオ事業部

    2010 - 2012

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Research History

  • Niigata University   Specially Appointed Assistant Professor

    2024.1

  • Niigata University   Institute for Research Promotion   Specially Appointed Assistant Professor

    2020.7 - 2022.3

  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2020.5 - 2020.6

  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2017.10 - 2020.5

Education

  • Gifu University   Graduate School, Division of Agriculture   生物資源科学専攻

    - 2017.3

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    Country： Japan

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Professional Memberships

  • Japanese Association for Laboratory Animal Science

    2024.10

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  • Japanese Association for Experimental Animal Technologists

    2022

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  • Japanese Biological Safety Association

    2021

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  • Japan Society for Marmoset Research

    2018

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Committee Memberships

  • The Japanese Biological Safety Association   学術企画委員会  

    2025.7   

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    Committee type：Academic society

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  • The Japanese Biological Safety Association   バイオセーフティ編集委員会  

    2025.7   

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    Committee type：Academic society

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  • Public Relations Committee,The Japanese Biological Safety Association   Chairperson  

    2025.4   

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    Committee type：Academic society

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  • The Japanese Biological Safety Association   Director  

    2025.1   

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Papers

  • Carbon Monoxide as a Molecular Modulator of Ischemia–Reperfusion Injury: New Insights for Translational Application in Organ Transplantation Reviewed

    Zhouyu Li, Kazuhiro Takeuchi, Yuichi Ariyoshi, Akira Kondo, Takehiro Iwanaga, Yurika Ichinari, Akiyuki Iwamoto, Kenya Shimizu, Kohei Miura, Shiori Miura, Lina Ma, Mitsuhiro Sekijima, Masayoshi Okumi, Hisashi Sahara

    International Journal of Molecular Sciences   26 ( 16 )   7825 - 7825   2025.8

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    Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Carbon monoxide (CO) is generally recognized as a toxic gas; however, it has recently been identified as an endogenous gasotransmitter with significant cytoprotective properties. CO modulates key molecular pathways, including anti-inflammatory, anti-apoptotic, antioxidant, and vasodilatory signaling pathways, by targeting heme- and non-heme-containing proteins. These proteins include soluble guanylate cyclase, cytochrome P450 enzymes, MAPKs, and NLRP3. This review summarizes recent advances in understanding the molecular mechanisms associated with the protective effects of CO, particularly in the context of ischemia–reperfusion injury relevant to organ transplantation. We discuss preclinical data from rodent and large animal models, as well as therapeutic delivery strategies, such as inhalation, CO-releasing molecules, and gas-entrapping materials. We also reviewed early-phase clinical trials. The objective of this review is to provide a thorough exploration of CO as a potential therapeutic gas, with special emphasis on its application in transplantation.

    DOI： 10.3390/ijms26167825

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  • Biosafety and Biosecurity Management in Spain Reviewed

    Shiori Miura

    Biosafety   2 ( 1 )   19 - 25   2025.3

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    Authorship：Lead author  

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  • Production of marmoset eggs and embryos from xenotransplanted ovary tissues

    Runa Hirayama, Hiroaki Taketsuru, Ena Nakatsukasa, Rie Natsume, Nae Saito, Shuko Adachi, Sayaka Kuwabara, Jun Miyamoto, Shiori Miura, Nobuyoshi Fujisawa, Yoshitaka Maeda, Keizo Takao, Manabu Abe, Toshikuni Sasaoka, Kenji Sakimura

    Scientific Reports   13 ( 1 )   2023.10

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    The common marmoset (Callithrix jacchus) has attracted attention as a valuable primate model for the analysis of human diseases. Despite the potential for primate genetic modification, however, its widespread lab usage has been limited due to the requirement for a large number of eggs. To make up for traditional oocyte retrieval methods such as hormone administration and surgical techniques, we carried out an alternative approach by utilizing ovarian tissue from deceased marmosets that had been disposed of. This ovarian tissue contains oocytes and can be used as a valuable source of follicles and oocytes. In this approach, the ovarian tissue sections were transplanted under the renal capsules of immunodeficient mice first. Subsequent steps consist of development of follicles by hormone administrations, induction of oocyte maturation and fertilization, and culture of the embryo. This method was first established with rat ovaries, then applied to marmoset ovaries, ultimately resulting in the successful acquisition of the late-stage marmoset embryos. This approach has the potential to contribute to advancements in genetic modification research and disease modeling through the use of primate models, promoting biotechnology with non-human primates and the 3Rs principle in animal experimentation.

    DOI： 10.1038/s41598-023-45224-x

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    Other Link： https://www.nature.com/articles/s41598-023-45224-x

  • Online Systems for the Management of Pathogens

    Shiori Miura, Kuni Sueyoshi, Katsuya Fukami

    JBSA Newsletter   13 ( 1 )   18 - 24   2023.3

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    Authorship：Lead author  

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  • 新潟大学における研究リスク管理 病原体等の適正管理のための取り組み

    三浦 詩織, 深見 克哉, 相馬 恵, 野水 和美, 中山 亮, 笹岡 俊邦, 神吉 智丈, 松本 壮吉, 末吉 邦

    新潟医学会雑誌   136 ( 4 )   117 - 126   2022.4

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  • Establishment of a Long-Term Survival Swine Model for the Observation of Transplanted Islets: a Preliminary Step in an Allogeneic Transplant Experiment International journal

    Kohei Miura, Takashi Kobayashi, Zhengkun Zhang, Pankaj Prasoon, Yuki Hirose, Hirosuke Ishikawa, Kazuyasu Takizawa, Jun Sakata, Shiori Miura, Toshikuni Sasaoka, Toshifumi Wakai

    Transplantation Proceedings   54 ( 2 )   507 - 512   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    BACKGROUND: Evaluation of an experimental and preclinical islet transplantation (IsletTx) model to elucidate associated clinical problems is vital. This study aimed to introduce a simple methodology for producing a swine autologous IsletTx model as a preliminary step in an allogeneic transplant experiment. METHODS AND MATERIALS: Twenty-seven pigs were included in the study. Total pancreatectomy (TP) was performed in 8 pigs (TP group), TP with autologous IsletTx in 9 (TP + IsletTx group), and distal pancreatectomy (DP) with autologous IsletTx in 10 (DP + IsletTx group). An open biopsy was performed on all pigs during postoperative day 14 using an infrared imaging (IRI) system. Laboratory data and postoperative survival were analyzed and compared according to the procedures done. RESULTS: Postoperative survival rate was significantly higher in the pigs with autologous IsletTx than in those without (P = .026). There were no significant differences in survival between the TP + IsletTx and DP + IsletTx groups (P = .746). Significant hyperglycemia was not observed in both groups, but the DP + IsletTx group remained relatively stable throughout the postoperative course. There were no differences in serum creatinine, aspartate aminotransferase, and alanine aminotransferase levels between the 2 groups. By selective liver lobe transplantation and administration of the IRI system, localization of the transplanted islets via open biopsy was achieved. CONCLUSIONS: We successfully developed an autologous IsletTx model and an open biopsy system using a swine model. This study will aid in the development of an allogeneic IsletTx experiment that may improve transplantation outcomes.

    DOI： 10.1016/j.transproceed.2021.10.029

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  • Effects of carbon monoxide on early dysfunction and microangiopathy following GalT-KO porcine pulmonary xenotransplantation in cynomolgus monkeys Reviewed

    Hisashi Sahara, Mitsuhiro Sekijima, Yuichi Ariyoshi, Akihiro Kawai, Kohei Miura, Shiori Waki, Louras Nathan, Yusuke Tomita, Takehiro Iwanaga, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Date, Hiroshi Nagashima, Akira Shimizu, Kazuhiko Yamada

    Xenotransplantation   25 ( 1 )   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Inc.  

    DOI： 10.1111/xen.12359

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  • Evaluation of the physiology of miniature pig fed Shochu distillery waste using mRNA expression profiling Reviewed

    Shiori Miura, Junko Takahashi, Hitoshi Iwahashi

    Journal of Material Cycles and Waste Management   20 ( 1 )   237 - 244   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10163-016-0567-z

    Web of Science

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  • The role of transcriptomics: physiological equivalence based on gene expression profiles Reviewed

    Miura S, Himaki T, Takahashi J, Iwahashi H

    Reviews in Agricultural Science   5   21 - 35   2017

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  • Development of the Intestinal Transplantation Model With Major Histocompatibility Complex Inbred CLAWN Miniature Swine Reviewed

    K. Miura, H. Sahara, S. Waki, A. Kawai, M. Sekijima, T. Kobayashi, Z. Zhang, T. Wakai, A. Shimizu, K. Yamada

    TRANSPLANTATION PROCEEDINGS   48 ( 4 )   1315 - 1319   2016.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.transproceed.2016.01.023

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  • Protective Effect of Neutralization of the Extracellular High-Mobility Group Box 1 on Renal Ischemia-Reperfusion Injury in Miniature Swine Reviewed

    Kohei Miura, Hisashi Sahara, Mitsuhiro Sekijima, Akihiro Kawai, Shiori Waki, Hiroaki Nishimura, Kentaro Setoyama, Eric S. Clayman, Akira Shimizu, Kazuhiko Yamada

    TRANSPLANTATION   98 ( 9 )   937 - 943   2014.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1097/TP.0000000000000358

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  • Results of Life-Supporting Galactosyltransferase Knockout Kidneys in Cynomolgus Monkeys Using Two Different Sources of Galactosyltransferase Knockout Swine Reviewed

    Mitsuhiro Sekijima, Shiori Waki, Hisashi Sahara, Masayuki Tasaki, Robert A. Wilkinson, Vincenzo Villani, Yoshiki Shimatsu, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Nagashima, Jay A. Fishman, Akira Shimizu, Kazuhiko Yamada

    TRANSPLANTATION   98 ( 4 )   419 - 426   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1097/TP.0000000000000314

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  • Production of cloned NIBS (Nippon Institute for Biological Science) and alpha-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates Reviewed

    Yoshiki Shimatsu, Kazuhiko Yamada, Wataru Horii, Atsushi Hirakata, Yuji Sakamoto, Shiori Waki, Junichi Sano, Toshiki Saitoh, Hisashi Sahara, Akira Shimizu, Hajime Yazawa, David H. Sachs, Tetsuo Nunoya

    XENOTRANSPLANTATION   20 ( 3 )   157 - 164   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/xen.12031

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  • Oligonucleotide Microarray Analysis of Dietary-Induced Hyperlipidemia Gene Expression Profiles in Miniature Pigs Reviewed

    Junko Takahashi, Shiori Waki, Rena Matsumoto, Junji Odake, Takayuki Miyaji, Junichi Tottori, Takehiro Iwanaga, Hitoshi Iwahashi

    PLOS ONE   7 ( 5 )   e37581   2012.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0037581

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MISC

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Presentations

  • Production of marmoset oocytes in mice: Current status and challenges in ovarian xenotransplantation.

    26th Japanese Society for Xenotransplantation  2024.2 

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    Event date： 2024.2

    Presentation type：Oral presentation (general)  

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  • Approaches to Establish a System for the Appropriate Management of Pathogens at Niigata University

    Shiori Miura, Katsuya Fukami, Kazumi Nomizu, Megumi Soma, Ryo Nakayama, Toshikuni Sasaoka, Tomotake Kanki, Kuni Sueyoshi

    20th The Japanese Biological Safety Association  2021.12 

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    Event date： 2021.12

    Presentation type：Oral presentation (general)  

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  • Generation of functional oocytes of common marmoset by xeno-transplantation of ovarian tissue. International conference

    Miura S, Maeda Y, Miyamoto J, Nakatsukasa E, Fujisawa N, Nakamura K, Sakimura K, Sasaoka T

    International symposium on animal production and conservation for sustainable development  2018.10 

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    Language：English   Presentation type：Symposium, workshop panel (public)  

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  • Efficacious combination of substrate and medium for feeder-free culture of naïve porcine iPS. International conference

    Waki (Miura) S, Sahara H, Mitsui K, Sekijima M, Murokawa T, Iwanaga T, Ichinari,Y, Kosai K, Yamada K

    26th International Congress of the Transplantation Society  2016.8 

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    Language：English   Presentation type：Poster presentation  

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  • Establishment of feeder free culture of naive state of porcine iPS cells. International conference

    Sahara H, Waki (Miura) S, Mitsui K, Sekijima M, Iwanaga T, Ichinari Y, Kosai K, Yamada K

    IPITA - IXA - CTS 2015 Joint Congress  2015.11 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Development of the intestinal transplantation model with MHC inbred CLAWN miniature swine. International conference

    Miura K, Sahara H, Waki (Miura) S, Kawai A, Sekijima M, Wakai T, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Beneficial effects of hydrogen sulfide on reducing ischemia-reperfusion injury and prolonging pulmonary allograft survival of fully MHC-disparate lung in CLAWN miniature swine. International conference

    Sahara H, Sekijima M, Ichinari Y, Iwanaga T, Waki (Miura) S, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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  • MHC-inbred CLAWN miniature swine as preclinical large animal model for transplantation. International conference

    Sahara H, Iwanaga T, Sekijima M, Waki (Miura) S, Ichinari Y, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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  • Hydrogen sulfide (H2S) prevents ischemia‐reperfusion injury (IRI) and prolongs survival of the fully MHC‐disparate lung graft in CLAWN miniature swine. International conference

    Sahara H, Sekijima M, Waki (Miura) S, Ichinari Y, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 35th Annual Meeting.  2015.4 

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  • Carbon monoxide attenuates hyperacute dysfunction and microangiopathy in GalT‐KO pulmonary xenotransplantation. International conference

    Sahara H, Nagashima H, Sekijima M, Miura K, Kawai A, Waki (Miura) S, Nakano K, Matsunari H, Date H, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 35th Annual Meeting.  2015.4 

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  • Hydrogen sulfide therapy prolongs survival of the fully MHC-disparate lung graft from brain-dead donors in CLAWN miniature swine. International conference

    Sahara H, Miura K, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    World Transplant Congress 2014  2014.7 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Establishment of clinically applicable strategy of CO therapy - Beneficial effects of inhaled carbon monoxide to brain-dead donors on prolonging pulmonary allograft survival in MHC-inbred CLAWN miniature swine. International conference

    Sahara H, Miura K, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    World Transplant Congress 2014  2014.7 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Medical gas (carbon monoxide, hydrogen sulfide) therapy prolongs survival of the fully MHC-disparate lung graft from brain-dead donors in miniature swine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 34th Annual Meeting.  2014.4 

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  • Attenuation of hyperacute dysfunction and microangiopathy by the treatment of carbon monoxide in GalT-KO pulmonary xenotransplantation. International conference

    Sahara H, Nagashima H, Miura K, Waki (Miura) S, Kawai A, Nakano K, Matsunari H, Arai Y, Sekijima M, Tasaki M, Setoyama K, Shimizu A, Date H, Yamada K

    12th Congress IXA (International Xenotransplantation Association 2013)  2013.11 

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  • Results of GalT-KO kidney transplantation in nonhuman primate using two different sources of donors, MGH/Nibs GalT-KO pigs and Meiji GalT-KO pigs at Kagoshima University. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Sekijima M, Tasaki M, Shimizu A, Nakano K, Matsunari H, Arai Y, Nagashima H, Yamada K

    The 2nd Symposium of the East Asia Xenotransplantation Association  2013.11 

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  • Porcine CMV may be the causative agent of porcine kidney rejection in GalT-KO pig to nonhuman primate preclinical xenotransplantation. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Sekijima M, Nakano K, Matsunari H, Arai Y, Tasaki M, Shimizu A, Nagashima H, Yamada K

    12th Congress IXA (International Xenotransplantation Association 2013)  2013.11 

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  • Prolongation of xenogeneic islet function using an HGF-based immunosuppressive regimen in a CLAWN miniature swine-to-cynomolgous monkey model. International conference

    Kawai A, Sahara H, Tasaki M, Okitsu T, Oku M, Nishimura H, Sekijima M, Miura K, Waki (Miura) S, Setoyama K, Shimizu A, Yamada K

    he 2nd Symposium of the East Asia Xenotransplantation Association.  2013.11 

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  • Porcine CMV may be the causative agent of porcine kidney rejection in GalT-KO pig to nonhuman primate preclinical xenotransplantation. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Tasaki M, Sekijima M, Arai Y, Matsunari H, Nakano K, Shimizu A, Nagashima H, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Potential benefit of subnormothermic preservation for transplanting kidneys which experience extended warm ischemic time in large animal model. International conference

    Kawai A, Sahara H, Sekijima M, Miura K, Waki (Miura) S, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • MHC-inbred CLAWN miniature swine is a highly valuable preclinical model for transplantation and regenerative medicine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Okumi M, Sekijima M, Tasaki M, Oku M, Nishimura H, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Beneficial effects of inhaled carbon monoxide (CO) to brain-dead (BD) donors on prolonging pulmonary allograft survival in MHC-inbred CLAWN miniature swine. International conference

    Miura K, Sahara H, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Carbon monoxide (CO) inhalation prolongs survival of the fully MHC-disparate lung graft from brain death donors in miniature swine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Sekijima M, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 33rd Annual Meeting  2013.4 

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  • The Effect of Carbon Dioxide to Yeast Saccharomyces cerevisiae. International conference

    Waki (Miura) S, Hachisuka Y, Nomura K, Iwahashi H, Matsuoka H, Kawachi S, Suzuki Y, Tamura K

    7th International conference on High Pressure Bioscience and Biotechnology (HPBB2012)  2012.10 

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Awards

  • Best Presentation Award

    2024.2   26th Japanese Society for Xenotransplantation  

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  • Best Oral Presentation Award 2013

    2013   日本異種移植研究会  

    三浦 詩織

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  • Young Investigator Travel Award

    2013   12th Congress IXA (International Xenotransplantation Association 2013)  

    MIURA Shiori

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  • Commendation from the United Graduate school of Agricultural Science, Gifu University

    2013   Gifu University  

    Shiori Miura

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  • Best Oral Presentation Award

    2012   HPBB2012 (High Pressure Bioscience and Biotechnology)   The Effect of Carbon Dioxide to Yeast Saccharomyces cerevisiae.

    MIURA Shiori

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Research Projects

  • 卵巣異種移植の成績を飛躍的に改善するカプセル化卵巣の開発

    Grant number：23K15831

    2023.4 - 2027.3

    System name：科学研究費助成事業

    Research category：若手研究

    Awarding organization：日本学術振興会

    三浦 詩織

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

    女性疾患の患者の卵巣凍結と移植は妊孕性温存のための唯一の手段である。凍結卵巣の保存および移植後の生着効率を向上させることを目的として、凍結卵巣の免疫不全マウスへの異種移植が行われており、患者に対する負担や、がん細胞の再播種のリスクを回避する新たな妊孕性温存の治療法として注目されている。しかし、移植後に、卵巣組織の損傷と原始卵胞の大量喪失が起こるため、異種移植の遠隔成績は極めて低い。
    申請者らはこれまで、マーモセット卵巣のマウスへの異種移植実験を実施してきている。すなわち、卵巣を除去したヌードマウスの腎被膜下にマーモセットの卵巣を異種移植し、ヌードマウスの性周期の再開や卵巣切片の生着、卵胞の発育を確認したほか、卵胞から採取した卵子による体外受精および胚発生に成功している。本研究では、移植成績を改善する手段として、脱細胞化組織 (Decellularized Tissue; DT)に着目した。移植組織の生着と機能回復に必要な「複雑な脈管構造」と「免疫防御機能」を持ち合わせているDTを卵巣異種移植に応用することで、移植組織の虚血障害と免疫反応による生着不良を克服し、移植組織の生着および機能回復の促進を目指す。
    令和5年度はブタ卵巣を用いて脱細胞化の実験プロトコルの最適化をすすめている。化学的方法による脱細胞化を行い、含有DNA量の測定や組織学的評価を実施している。
    ヌードマウスへの卵巣の異種移植については、入手数が少ないマーモセット卵巣の代替としてブタ卵巣を用いて移植条件を検討中である。
    また、卵巣異種移植の現状を調査し、その結果および実施した研究成果を第26回日本異種移植研究会にて発表し、優秀演題賞を受賞した（演題名「マーモセット卵子をマウス体内で作る～卵巣異種移植の現状と課題～」）。

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  • 脂質メディエータS1Pの血管新生作用を応用した新たな移植膵島生着率改善の試み

    Grant number：23K08085

    2023.4 - 2026.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

    三浦 宏平, 小林 隆, 坂田 純, 滝沢 一泰, 石川 博補, 安部 舜, 河内 裕介, 若井 俊文, 三浦 詩織

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    2023年度は、In vitro実験としてブタ膵臓よりRicordi法で膵島を分離し、UW液にて保存することで、ブタ膵島の分離と長時間の保存が可能であることを確認した。In vivo実験として6頭のブタ自家膵島移植モデルを作製した。20kgのブタで膵亜全摘術（80％膵切除）および脾臓摘出術を実施した。切除膵の主膵管から逆行性にコラゲナーゼを注入し膵実質を膨化させたのち、Ricordi法にて膵島を分離した。ドナーブタの脾静脈からカニュレーションし、分離した膵島を膵島混濁液として経門脈的に肝内に自家移植した。本モデルをコントロール群（n=2）とし、FTY720 0.5mg/kgを膵島移植後5日間連続で経静脈投与するFTY群（n=4）を作成した。28日間生存モデルとし、両者の血糖値とケトン値の連日の推移、活動性、および移植後28日目の経静脈ブドウ糖負荷試験の結果を比較した。また、移植後28日目に摘出した肝臓の組織に生着する膵島数を測定するため、現在標本を作製中である。

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  • Overseas Dispatch Support Program

    2023 - 2024

    Awarding organization：Japanese Biological Safety Association

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  • Preclinical study on protection strategy and function recovery strategy of preimplantation for marginal graft

    Grant number：26293355

    2014.4 - 2017.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Kusaka Mamoru, HOSHINAGA Kiyotaka, MIURA Kouhei, KAWAI Akihiro, WAKI Shiori

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    Grant amount：\16250000 （ Direct Cost: \12500000 、 Indirect Cost：\3750000 ）

    In this study, we focused on agents with various cytoprotective effects on marginal donor kidneys where activation of inflammatory precursors caused tissue injury. Using established MHC miniture swine which can evaluate detailed immune reaction, we investigated the effect of organ protection and repair by drug administration to donor, recipient, graft persistent perfusion preservation. We conducted experiments to establish a strategy to restore and recover marginal donor organs to conditions comparable to living organs.

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