Updated on 2024/04/27

写真a

 
MIURA Shiori
 
Organization
. . Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
External link

Degree

  • 博士 (農学) ( 2017.3   岐阜大学 )

Research Interests

  • Biosafety

  • Biorisk management

  • 移植学

  • 実験動物学

  • 遺伝子発現解析

Research Areas

  • Life Science / Laboratory animal science

  • Life Science / Molecular biology

Research History (researchmap)

  • Niigata University   Institute for Research Administration   Specially Appointed Assistant Professor

    2020.7

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  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2017.10 - 2020.6

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  • Kagoshima University   Center for Advanced Biomedical Science and Swine Research

    2012 - 2015

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  • 株式会社しか屋   バイオ事業部

    2010 - 2012

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Research History

  • Niigata University   Specially Appointed Assistant Professor

    2024.1

  • Niigata University   Institute for Research Promotion   Specially Appointed Assistant Professor

    2020.7 - 2022.3

  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2020.5 - 2020.6

  • Niigata University   Brain Research Institute Center for Bioresources   Specially Appointed Assistant

    2017.10 - 2020.5

Education

  • Gifu University   Graduate School, Division of Agriculture   生物資源科学専攻

    - 2017.3

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    Country： Japan

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Papers

  • Production of marmoset eggs and embryos from xenotransplanted ovary tissues

    Runa Hirayama, Hiroaki Taketsuru, Ena Nakatsukasa, Rie Natsume, Nae Saito, Shuko Adachi, Sayaka Kuwabara, Jun Miyamoto, Shiori Miura, Nobuyoshi Fujisawa, Yoshitaka Maeda, Keizo Takao, Manabu Abe, Toshikuni Sasaoka, Kenji Sakimura

    Scientific Reports   13 ( 1 )   2023.10

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    The common marmoset (Callithrix jacchus) has attracted attention as a valuable primate model for the analysis of human diseases. Despite the potential for primate genetic modification, however, its widespread lab usage has been limited due to the requirement for a large number of eggs. To make up for traditional oocyte retrieval methods such as hormone administration and surgical techniques, we carried out an alternative approach by utilizing ovarian tissue from deceased marmosets that had been disposed of. This ovarian tissue contains oocytes and can be used as a valuable source of follicles and oocytes. In this approach, the ovarian tissue sections were transplanted under the renal capsules of immunodeficient mice first. Subsequent steps consist of development of follicles by hormone administrations, induction of oocyte maturation and fertilization, and culture of the embryo. This method was first established with rat ovaries, then applied to marmoset ovaries, ultimately resulting in the successful acquisition of the late-stage marmoset embryos. This approach has the potential to contribute to advancements in genetic modification research and disease modeling through the use of primate models, promoting biotechnology with non-human primates and the 3Rs principle in animal experimentation.

    DOI： 10.1038/s41598-023-45224-x

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    Other Link： https://www.nature.com/articles/s41598-023-45224-x

  • Establishment of a Long-Term Survival Swine Model for the Observation of Transplanted Islets: a Preliminary Step in an Allogeneic Transplant Experiment International journal

    Kohei Miura, Takashi Kobayashi, Zhengkun Zhang, Pankaj Prasoon, Yuki Hirose, Hirosuke Ishikawa, Kazuyasu Takizawa, Jun Sakata, Shiori Miura, Toshikuni Sasaoka, Toshifumi Wakai

    Transplantation Proceedings   54 ( 2 )   507 - 512   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    BACKGROUND: Evaluation of an experimental and preclinical islet transplantation (IsletTx) model to elucidate associated clinical problems is vital. This study aimed to introduce a simple methodology for producing a swine autologous IsletTx model as a preliminary step in an allogeneic transplant experiment. METHODS AND MATERIALS: Twenty-seven pigs were included in the study. Total pancreatectomy (TP) was performed in 8 pigs (TP group), TP with autologous IsletTx in 9 (TP + IsletTx group), and distal pancreatectomy (DP) with autologous IsletTx in 10 (DP + IsletTx group). An open biopsy was performed on all pigs during postoperative day 14 using an infrared imaging (IRI) system. Laboratory data and postoperative survival were analyzed and compared according to the procedures done. RESULTS: Postoperative survival rate was significantly higher in the pigs with autologous IsletTx than in those without (P = .026). There were no significant differences in survival between the TP + IsletTx and DP + IsletTx groups (P = .746). Significant hyperglycemia was not observed in both groups, but the DP + IsletTx group remained relatively stable throughout the postoperative course. There were no differences in serum creatinine, aspartate aminotransferase, and alanine aminotransferase levels between the 2 groups. By selective liver lobe transplantation and administration of the IRI system, localization of the transplanted islets via open biopsy was achieved. CONCLUSIONS: We successfully developed an autologous IsletTx model and an open biopsy system using a swine model. This study will aid in the development of an allogeneic IsletTx experiment that may improve transplantation outcomes.

    DOI： 10.1016/j.transproceed.2021.10.029

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  • Effects of carbon monoxide on early dysfunction and microangiopathy following GalT-KO porcine pulmonary xenotransplantation in cynomolgus monkeys Reviewed

    Hisashi Sahara, Mitsuhiro Sekijima, Yuichi Ariyoshi, Akihiro Kawai, Kohei Miura, Shiori Waki, Louras Nathan, Yusuke Tomita, Takehiro Iwanaga, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Date, Hiroshi Nagashima, Akira Shimizu, Kazuhiko Yamada

    Xenotransplantation   25 ( 1 )   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Inc.  

    Background: Despite progress in the current genetic manipulation of donor pigs, most non-human primates were lost within a day of receiving porcine lung transplants. We previously reported that carbon monoxide (CO) treatment improved pulmonary function in an allogeneic lung transplant (LTx) model using miniature swine. In this study, we evaluated whether the perioperative treatment with low-dose inhalation of CO has beneficial effects on porcine lung xenografts in cynomolgus monkeys (cynos). Methods: Eight cynos received orthotopic left LTx using either α-1,3-galactosyltransferase knockout (GalT-KO
    n = 2) or GalT-KO with human decay accelerating factor (hDAF) (GalT-KO/hDAF
    n = 6) swine donors. These eight animals were divided into three groups. In Group 1 (n = 2), neither donor nor recipients received CO therapy. In Group 2 (n = 4), donors were treated with inhaled CO for 180-minute. In Group 3 (n = 2), both donors and recipients were treated with CO (donor: 180-minute
    recipient: 360-minute). Concentration of inhaled CO was adjusted based on measured levels of carboxyhemoglobin in the blood (15%-20%). Results: Two recipients survived for 3 days
    75 hours (no-CO) and 80 hours (CO in both the donor and the recipient), respectively. Histology showed less inflammatory cell infiltrates, intravascular thrombi, and hemorrhage in the 80-hour survivor with the CO treatment than the 75-hours non-CO treatment. Anti–non-Gal cytotoxicity levels did not affect the early loss of the grafts. Although CO treatment did not prolong overall xeno lung graft survival, the recipient/donor CO treatment helped to maintain platelet counts and inhibit TNF-α and IL-6 secretion at 2 hours after revascularization of grafts. In addition, lung xenografts that were received recipient/donor CO therapy demonstrated fewer macrophage and neutrophil infiltrates. Infiltrating macrophages as well as alveolar epithelial cells in the CO-treated graft expressed heme oxygenase-1. Conclusion: Although further investigation is required, CO treatment may provide a beneficial strategy for pulmonary xenografts.

    DOI： 10.1111/xen.12359

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  • Evaluation of the physiology of miniature pig fed Shochu distillery waste using mRNA expression profiling Reviewed

    Shiori Miura, Junko Takahashi, Hitoshi Iwahashi

    Journal of Material Cycles and Waste Management   20 ( 1 )   237 - 244   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Tokyo  

    Shochu distillery waste is the discarded material generated during Shochu production, and it has been used as feed for livestock. In this study, we evaluated the applicability of Shochu distillery waste as a livestock diet. Shochu distillery waste diet was fed to Clawn miniature pigs. At the end of the dietary period, blood samples were collected for biochemical examination and microarray analysis. No significant differences were observed between the control and Shochu distillery waste treatment groups based on physical and biochemical examination. Gene expression patterns were also similar. In addition, gene profiling of these two groups was compared with those of hyperlipidemia and toxicant model groups. Expression profiling of the two groups was different from those of the hyperlipidemia and toxicant model groups. In conclusion, the Shochu distillery waste diet did not affect pig physiology and it is a suitable substitute for standard feed. Moreover, these results promote the potential for microarray analysis use in the evaluation of food safety.

    DOI： 10.1007/s10163-016-0567-z

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  • The role of transcriptomics: physiological equivalence based on gene expression profiles Reviewed

    Miura S, Himaki T, Takahashi J, Iwahashi H

    Reviews in Agricultural Science   5   21 - 35   2017

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  • Development of the Intestinal Transplantation Model With Major Histocompatibility Complex Inbred CLAWN Miniature Swine Reviewed

    K. Miura, H. Sahara, S. Waki, A. Kawai, M. Sekijima, T. Kobayashi, Z. Zhang, T. Wakai, A. Shimizu, K. Yamada

    TRANSPLANTATION PROCEEDINGS   48 ( 4 )   1315 - 1319   2016.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

    Background. Clinical intestinal transplantation (Int-Tx) is associated with some problems such as rejection, infection, graft-versus-host disease, and ischemia-reperfusion injury (IRI). To determine mechanisms of rejection as well as to develop treatment strategies for Int-Tx, this study was designed to establish both heterotopic and orthotropic Int-Tx models using major histocompatibility antigen complex (MHC) inbred CLAWN miniature swine.
    Materials and Methods. Eleven CLAWN miniature swine received MHC matched but minor antigen mismatched allogenic intestinal grafts. Four animals received intestinal grafts heterotopically and kept host intestine intact. The remaining 7 animals received intestinal grafts orthotopically and resected host small intestine. Continuous infusion of tacrolimus was given from day 0 for 12 days.
    Results. Heterotopically transplanted small intestine were well perfused after revascularization; however, grafts easily underwent ischemic changes during or soon after abdomen closure due to oppression of the grafts in the limited abdominal space. In contrast, all of 7 orthotopically transplanted intestinal grafts in which recipients' small intestine was removed from the jejunum to the ileum had no signs of severe ischemia associated with compartment syndrome. Elevation of the serum concentration of inflammatory cytokines and the progression of lethal acidosis seen in recipients of heterotipic transplantation were markedly less in the case of orthotopic transplantation. Two recipients survived more than 30 days, and 1 long-term survivor showed no evidence of rejection at day 90 despite the fact that tacrolimus was stopped at day 12.
    Conclusions. In this study, we demonstrated the establishment of a clinically relevant orthotopic Int-Tx model with long survival in MHC inbred CLAWN miniature swine. We believe that this unique MHC inbred swine Int-Tx model is useful for developing treatment strategies for clinical Int-Tx.

    DOI： 10.1016/j.transproceed.2016.01.023

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  • Protective Effect of Neutralization of the Extracellular High-Mobility Group Box 1 on Renal Ischemia-Reperfusion Injury in Miniature Swine Reviewed

    Kohei Miura, Hisashi Sahara, Mitsuhiro Sekijima, Akihiro Kawai, Shiori Waki, Hiroaki Nishimura, Kentaro Setoyama, Eric S. Clayman, Akira Shimizu, Kazuhiko Yamada

    TRANSPLANTATION   98 ( 9 )   937 - 943   2014.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：LIPPINCOTT WILLIAMS & WILKINS  

    Background Strategies that reduce ischemia-reperfusion injury (IRI) have the potential to expand the numbers of available organs for transplantation. Recent reports in rodent models have demonstrated that high-mobility group box 1 (HMGB1) acts as an alarm in initiating the inflammatory response resulting from ischemic injury. The aim of this study was to evaluate the cytoprotective effects of anti-HMGB1 antibodies on renal IRI in preclinical large animals.
    Methods One hundred twenty minutes of warm and 60 min of cold renal ischemia were induced in 8 CLAWN miniature swine. Three of eight animals received intravenous anti-HMGB1 antibody at 1 mg/kg just before the reperfusion of renal blood flow. Renal function was assessed by serum creatinine and renal biopsy. Serum levels of interleukin (IL)-1, IL-6, and HMGB1 were measured.
    Results The concentration of HMGB1 increased as early as 30 min after reperfusion and before the elevation of IL-1 and IL-6. Serum creatinine levels were markedly elevated, peaking at a median of 5 days (peak creatinine levels: 11.61.6 mg/dL) and recovering by day 14. Anti-HMGB1 antibody injection dramatically decreased renal damage as well as serum levels of HMGB1 associated with IRI. Renal function returned to near normal by day 9, and peak creatinine levels were markedly lower (7.4 +/- 0.2 mg/dL), and biopsies possessed fewer pathologic changes when compared to the control group.
    Conclusion In this study, we demonstrated the beneficial effects of perioperative administration of anti-HMGB1 antibody in reducing renal IRI in a clinically relevant, large animal model.

    DOI： 10.1097/TP.0000000000000358

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  • Results of Life-Supporting Galactosyltransferase Knockout Kidneys in Cynomolgus Monkeys Using Two Different Sources of Galactosyltransferase Knockout Swine Reviewed

    Mitsuhiro Sekijima, Shiori Waki, Hisashi Sahara, Masayuki Tasaki, Robert A. Wilkinson, Vincenzo Villani, Yoshiki Shimatsu, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Nagashima, Jay A. Fishman, Akira Shimizu, Kazuhiko Yamada

    TRANSPLANTATION   98 ( 4 )   419 - 426   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：LIPPINCOTT WILLIAMS & WILKINS  

    Background. Various durations of survival have been observed in the xenotransplantation of life-supporting alpha-1,3-galactosyltransferase knockout (GalT-KO) porcine kidneys into nonhuman primates. Although others have demonstrated loss of GalT-KOV-transplanted kidneys within 2 weeks, we have reported an average survival of 51 days with the cotransplantation of the kidney and vascularized thymus and an average of 29 days with the kidney alone. To determine the factors responsible for this difference in survival time, we performed xenogeneic kidney transplantations into cynomolgus monkeys with an anti-CD40L/based regimen using two different strains of GalT-KO swine, one derived from MGH miniature swine and the other obtained from Meji University.
    Materials and Methods. Eight cynomolgus moneys received GalT-KO kidneys. Three kidney grafts were from Massachusetts General Hospital (MGH)-Nippon Institute for Biological Science (NIBS) GalT-KO pigs and five GalT-KO grafts were from MEIJI GalT-KO swine. All cynomolgus recipients were treated identically.
    Results. Recipients of kidneys from the MGH GalT-KO kidneys swine, produced by nuclear transfer in Japan, survived an average of 28.7 days, whereas recipients of MEIJI GalT-KO kidneys swine survived an average of 9.2 days. Among the differences between these two groups, one potentially revealing disparity was that the MEIJI swine were positive for porcine cytomegalovirus, whereas the MGH-derived swine were negative.
    Conclusion. This is the first study comparing renal xenotransplantation from two different sources of GalT-KO swine into nonhuman primates at a single center. The results demonstrate that porcine cytomegalovirus may be responsible for early loss of GalT-KO swine kidney xenografts.

    DOI： 10.1097/TP.0000000000000314

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  • Production of cloned NIBS (Nippon Institute for Biological Science) and alpha-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates Reviewed

    Yoshiki Shimatsu, Kazuhiko Yamada, Wataru Horii, Atsushi Hirakata, Yuji Sakamoto, Shiori Waki, Junichi Sano, Toshiki Saitoh, Hisashi Sahara, Akira Shimizu, Hajime Yazawa, David H. Sachs, Tetsuo Nunoya

    XENOTRANSPLANTATION   20 ( 3 )   157 - 164   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

    Background Nuclear transfer (NT) technologies offer a means for producing the genetically modified pigs necessary to develop swine models for mechanistic studies of disease processes as well as to serve as organ donors for xenotransplantation. Most previous studies have used commercial pigs as surrogates. Method and Results In this study, we established a cloning technique for miniature pigs by somatic cell nuclear transfer (SCNT) using Nippon Institute for Biological Science (NIBS) miniature pigs as surrogates. Moreover, utilizing this technique, we have successfully produced an -1, 3-galactosyltransferase knockout (GalT-KO) miniature swine. Fibroblasts procured from a NIBS miniature pig fetus were injected into 1312 enucleated oocytes. The cloned embryos were transferred to 11 surrogates of which five successfully delivered 13 cloned offspring; the production efficiency was 1.0% (13/1312). In a second experiment, lung fibroblasts obtained from neonatal GalT-KO MGH miniature swine were used as donor cells and 1953 cloned embryos were transferred to 12 surrogates. Six cloned offspring were born from five surrogates, a production efficiency of 0.3% (6/1953). Conclusions These results demonstrate successful establishment of a miniature pig cloning technique by SCNT using NIBS miniature pigs as surrogates. To our knowledge, this is the first demonstration of successful production of GalT-KO miniature swine using miniature swine surrogates. This technique could help to ensure a stable supply of the cloned pigs through the use of miniature pig surrogates and could expand production in countries with limited space or in facilities with special regulations such as specific pathogen-free or good laboratory practice.

    DOI： 10.1111/xen.12031

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  • Oligonucleotide Microarray Analysis of Dietary-Induced Hyperlipidemia Gene Expression Profiles in Miniature Pigs Reviewed

    Junko Takahashi, Shiori Waki, Rena Matsumoto, Junji Odake, Takayuki Miyaji, Junichi Tottori, Takehiro Iwanaga, Hitoshi Iwahashi

    PLOS ONE   7 ( 5 )   e37581   2012.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

    Background: Hyperlipidemia animal models have been established, but complete gene expression profiles of the transition from normal lipid levels have not been obtained. Miniature pigs are useful model animals for gene expression studies on dietary-induced hyperlipidemia because they have a similar anatomy and digestive physiology to humans, and blood samples can be obtained from them repeatedly.
    Methodology: Two typical dietary treatments were used for dietary-induced hyperlipidemia models, by using specific pathogen-free (SPF) Clawn miniature pigs. One was a high-fat and high-cholesterol diet (HFCD) and the other was a high-fat, high-cholesterol, and high-sucrose diet (HFCSD). Microarray analyses were conducted from whole blood samples during the dietary period and from white blood cells at the end of the dietary period to evaluate the transition of expression profiles of the two dietary models.
    Principal Findings: Variations in whole blood gene expression intensity within the HFCD or the HFCSD group were in the same range as the controls provide with normal diet at all periods. This indicates uniformity of dietary-induced hyperlipidemia for our dietary protocols. Gene ontology-(GO) based functional analyses revealed that characteristics of the common changes between HFCD and HFCSD were involved in inflammatory responses and reproduction. The correlation coefficient between whole blood and white blood cell expression profiles at 27 weeks with the HFCSD diet was significantly lower than that of the control and HFCD diet groups. This may be due to the effects of RNA originating from the tissues and/or organs.
    Conclusions: No statistically significant differences in fasting plasma lipids and glucose levels between the HFCD and HFCSD groups were observed. However, blood RNA analyses revealed different characteristics corresponding to the dietary protocols. In this study, whole blood RNA analyses proved to be a useful tool to evaluate transitions in dietary-induced hyperlipidemia gene expression profiles in miniature pigs.

    DOI： 10.1371/journal.pone.0037581

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Presentations

  • Production of marmoset oocytes in mice: Current status and challenges in ovarian xenotransplantation.

    26th Japanese Society for Xenotransplantation  2024.2 

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    Event date： 2024.2

    Presentation type：Oral presentation (general)  

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  • Approaches to Establish a System for the Appropriate Management of Pathogens at Niigata University

    Shiori Miura, Katsuya Fukami, Kazumi Nomizu, Megumi Soma, Ryo Nakayama, Toshikuni Sasaoka, Tomotake Kanki, Kuni Sueyoshi

    20th The Japanese Biological Safety Association  2021.12 

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    Event date： 2021.12

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  • Generation of functional oocytes of common marmoset by xeno-transplantation of ovarian tissue. International conference

    Miura S, Maeda Y, Miyamoto J, Nakatsukasa E, Fujisawa N, Nakamura K, Sakimura K, Sasaoka T

    International symposium on animal production and conservation for sustainable development  2018.10 

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    Language：English   Presentation type：Symposium, workshop panel (public)  

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  • Efficacious combination of substrate and medium for feeder-free culture of naïve porcine iPS. International conference

    Waki (Miura) S, Sahara H, Mitsui K, Sekijima M, Murokawa T, Iwanaga T, Ichinari,Y, Kosai K, Yamada K

    26th International Congress of the Transplantation Society  2016.8 

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  • Establishment of feeder free culture of naive state of porcine iPS cells. International conference

    Sahara H, Waki (Miura) S, Mitsui K, Sekijima M, Iwanaga T, Ichinari Y, Kosai K, Yamada K

    IPITA - IXA - CTS 2015 Joint Congress  2015.11 

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  • Development of the intestinal transplantation model with MHC inbred CLAWN miniature swine. International conference

    Miura K, Sahara H, Waki (Miura) S, Kawai A, Sekijima M, Wakai T, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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  • MHC-inbred CLAWN miniature swine as preclinical large animal model for transplantation. International conference

    Sahara H, Iwanaga T, Sekijima M, Waki (Miura) S, Ichinari Y, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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  • Beneficial effects of hydrogen sulfide on reducing ischemia-reperfusion injury and prolonging pulmonary allograft survival of fully MHC-disparate lung in CLAWN miniature swine. International conference

    Sahara H, Sekijima M, Ichinari Y, Iwanaga T, Waki (Miura) S, Shimizu A, Yamada K

    The 14th Congress of the Asian Society of Transplantation.  2015.8 

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  • Hydrogen sulfide (H2S) prevents ischemia‐reperfusion injury (IRI) and prolongs survival of the fully MHC‐disparate lung graft in CLAWN miniature swine. International conference

    Sahara H, Sekijima M, Waki (Miura) S, Ichinari Y, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 35th Annual Meeting.  2015.4 

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  • Carbon monoxide attenuates hyperacute dysfunction and microangiopathy in GalT‐KO pulmonary xenotransplantation. International conference

    Sahara H, Nagashima H, Sekijima M, Miura K, Kawai A, Waki (Miura) S, Nakano K, Matsunari H, Date H, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 35th Annual Meeting.  2015.4 

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  • Hydrogen sulfide therapy prolongs survival of the fully MHC-disparate lung graft from brain-dead donors in CLAWN miniature swine. International conference

    Sahara H, Miura K, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    World Transplant Congress 2014  2014.7 

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  • Establishment of clinically applicable strategy of CO therapy - Beneficial effects of inhaled carbon monoxide to brain-dead donors on prolonging pulmonary allograft survival in MHC-inbred CLAWN miniature swine. International conference

    Sahara H, Miura K, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    World Transplant Congress 2014  2014.7 

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  • Medical gas (carbon monoxide, hydrogen sulfide) therapy prolongs survival of the fully MHC-disparate lung graft from brain-dead donors in miniature swine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 34th Annual Meeting.  2014.4 

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  • Attenuation of hyperacute dysfunction and microangiopathy by the treatment of carbon monoxide in GalT-KO pulmonary xenotransplantation. International conference

    Sahara H, Nagashima H, Miura K, Waki (Miura) S, Kawai A, Nakano K, Matsunari H, Arai Y, Sekijima M, Tasaki M, Setoyama K, Shimizu A, Date H, Yamada K

    12th Congress IXA (International Xenotransplantation Association 2013)  2013.11 

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  • Results of GalT-KO kidney transplantation in nonhuman primate using two different sources of donors, MGH/Nibs GalT-KO pigs and Meiji GalT-KO pigs at Kagoshima University. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Sekijima M, Tasaki M, Shimizu A, Nakano K, Matsunari H, Arai Y, Nagashima H, Yamada K

    The 2nd Symposium of the East Asia Xenotransplantation Association  2013.11 

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  • Porcine CMV may be the causative agent of porcine kidney rejection in GalT-KO pig to nonhuman primate preclinical xenotransplantation. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Sekijima M, Nakano K, Matsunari H, Arai Y, Tasaki M, Shimizu A, Nagashima H, Yamada K

    12th Congress IXA (International Xenotransplantation Association 2013)  2013.11 

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  • Prolongation of xenogeneic islet function using an HGF-based immunosuppressive regimen in a CLAWN miniature swine-to-cynomolgous monkey model. International conference

    Kawai A, Sahara H, Tasaki M, Okitsu T, Oku M, Nishimura H, Sekijima M, Miura K, Waki (Miura) S, Setoyama K, Shimizu A, Yamada K

    he 2nd Symposium of the East Asia Xenotransplantation Association.  2013.11 

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  • Porcine CMV may be the causative agent of porcine kidney rejection in GalT-KO pig to nonhuman primate preclinical xenotransplantation. International conference

    Waki (Miura) S, Sahara H, Miura K, Kawai A, Tasaki M, Sekijima M, Arai Y, Matsunari H, Nakano K, Shimizu A, Nagashima H, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Potential benefit of subnormothermic preservation for transplanting kidneys which experience extended warm ischemic time in large animal model. International conference

    Kawai A, Sahara H, Sekijima M, Miura K, Waki (Miura) S, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • MHC-inbred CLAWN miniature swine is a highly valuable preclinical model for transplantation and regenerative medicine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Okumi M, Sekijima M, Tasaki M, Oku M, Nishimura H, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Beneficial effects of inhaled carbon monoxide (CO) to brain-dead (BD) donors on prolonging pulmonary allograft survival in MHC-inbred CLAWN miniature swine. International conference

    Miura K, Sahara H, Waki (Miura) S, Kawai A, Shimizu A, Yamada K

    The 13th Congress of the Asian Society of Transplantation  2013.9 

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  • Carbon monoxide (CO) inhalation prolongs survival of the fully MHC-disparate lung graft from brain death donors in miniature swine. International conference

    Sahara H, Miura K, Kawai A, Waki (Miura) S, Sekijima M, Shimizu A, Yamada K

    International Society for Heart and Lung Transplantation 33rd Annual Meeting  2013.4 

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    Language：English   Presentation type：Oral presentation (general)  

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  • The Effect of Carbon Dioxide to Yeast Saccharomyces cerevisiae. International conference

    Waki (Miura) S, Hachisuka Y, Nomura K, Iwahashi H, Matsuoka H, Kawachi S, Suzuki Y, Tamura K

    7th International conference on High Pressure Bioscience and Biotechnology (HPBB2012)  2012.10 

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    Language：English   Presentation type：Oral presentation (general)  

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Awards

  • Best Presentation Award

    2024.2   26th Japanese Society for Xenotransplantation  

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  • Best Oral Presentation Award 2013

    2013   日本異種移植研究会  

    三浦 詩織

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  • Young Investigator Travel Award

    2013   12th Congress IXA (International Xenotransplantation Association 2013)  

    MIURA Shiori

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  • Commendation from the United Graduate school of Agricultural Science, Gifu University

    2013   Gifu University  

    Shiori Miura

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  • Best Oral Presentation Award

    2012   HPBB2012 (High Pressure Bioscience and Biotechnology)   The Effect of Carbon Dioxide to Yeast Saccharomyces cerevisiae.

    MIURA Shiori

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Research Projects

  • 卵巣異種移植の成績を飛躍的に改善するカプセル化卵巣の開発

    Grant number：23K15831

    2023.4 - 2026.3

    System name：科学研究費助成事業

    Research category：若手研究

    Awarding organization：日本学術振興会

    三浦 詩織

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

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  • Overseas Dispatch Support Program

    2023 - 2024

    Awarding organization：Japanese Biological Safety Association

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  • Preclinical study on protection strategy and function recovery strategy of preimplantation for marginal graft

    Grant number：26293355

    2014.4 - 2017.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Kusaka Mamoru, HOSHINAGA Kiyotaka, MIURA Kouhei, KAWAI Akihiro, WAKI Shiori

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    Grant amount：\16250000 （ Direct Cost: \12500000 、 Indirect Cost：\3750000 ）

    In this study, we focused on agents with various cytoprotective effects on marginal donor kidneys where activation of inflammatory precursors caused tissue injury. Using established MHC miniture swine which can evaluate detailed immune reaction, we investigated the effect of organ protection and repair by drug administration to donor, recipient, graft persistent perfusion preservation. We conducted experiments to establish a strategy to restore and recover marginal donor organs to conditions comparable to living organs.

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