Publishing type：Research paper (scientific journal)   Publisher：American Dental Society of Anesthesiology (ADSA)  

DOI： 10.2344/anpr-70-03-02

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Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jds.2022.08.018

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Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)  

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Publishing type：Research paper (scientific journal)   Publisher：Cureus, Inc.  

DOI： 10.7759/cureus.33733

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can cause loss or alteration of taste and smell as early symptoms or sequelae, but the detailed mechanism behind this phenomenon remains unclear. Here, we investigated whether the SARS-CoV-2 spike protein induces taste cell apoptosis and expression of the apoptosis-related cytokine TNF-α in male Sprague-Dawley rats. Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-fluorescein nick end labeling (TUNEL) assay results revealed a significantly higher apoptosis index for taste cells in the SARS-CoV-2 group than for those in the control group. An immunohistochemistry analysis indicated significantly more TNF-α-positive cells in the SARS-CoV-2 group compared with the control group. These data suggest that the SARS-CoV-2 spike protein promotes taste cell apoptosis and the release of apoptosis-related cytokine TNF-α, implicating its contribution to the taste malfunction caused by coronavirus disease 2019 (COVID-19).

DOI： 10.1016/j.jds.2022.08.016

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections cause loss or alteration of taste and smell as early symptoms and sequelae, but the detailed mechanism remains unclear. This study investigated whether coronavirus disease 2019-induced taste disorders are caused by direct effects on taste bud cells. SARS-CoV-2 recombinant spike and nucleocapsid proteins were applied to circumvallate papillae of male Sprague-Dawley rats. Immunohistochemistry and image analysis were used to compare the number of taste buds, and taste bud cells and area, together with confirmation of angiotensin-converting enzyme 2 (ACE2) expression. Immunohistochemical analysis revealed ACE2 expression in the taste buds of rat circumvallate papillae. Decreases in the number of taste buds, taste bud cells, and their area were observed at 12 days after application of SARS-CoV-2 recombinant spike and nucleocapsid proteins. These data suggest that SARS-CoV-2 proteins induce degeneration of taste buds.

DOI： 10.1016/j.jds.2022.03.009

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jds.2021.10.015

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Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：日本歯科薬物療法学会  

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Publishing type：Research paper (scientific journal)  

DOI： 10.1002/jdd.12481

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Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)  

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/ca.23724

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Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)  

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Lithium carbonate is a medication used for the management of various mental disorders. The present report describes a case of prolongation of rocuronium bromide in a patient concurrently taking lithium carbonate. A 64-year-old woman was scheduled to undergo cystectomy under general anesthesia. The patient took lithium carbonate (600 mg/d) for treatment of bipolar affective disorder. General anesthesia was induced with propofol, fentanyl, remifentanil, and sevoflurane. After loss of consciousness, rocuronium bromide (50 mg) was administered, and the trachea was intubated. Approximately 1 hour after the administration of rocuronium, the degree of residual muscle relaxant was evaluated using a nerve stimulation device. No muscle contraction occurred with train-of-four (TOF) stimulation. Following administration of sugammadex (200 mg) the TOF ratio increased to 95%. The ionic size of lithium is similar to that of sodium; therefore, lithium is transported into the cell with sodium. The resting membrane potential decreases, leading to a reduction in the height of the action potential. Thus, the effect of the remaining lithium may have been superimposed on the rocuronium neuromuscular blockade. Evaluation with a nerve stimulation device in patients taking lithium is crucial before extubation because of the risk of rocuronium potentiation.

DOI： 10.2344/anpr-66-04-04

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jds.2019.11.007

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Language：English   Publishing type：Research paper (scientific journal)  

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Ltd  

Introduction: Every dental provider needs to be educated about medical emergencies to provide safe dental care. Simulation training is available with simulators such as advanced life support manikins and robot patients. However, the purchase and development costs of these simulators are high. We have developed a simulation training course on medical emergencies using an inexpensive software application. The purpose of this study was to evaluate the educational effectiveness of this course. Materials and Methods: Fifty-one dental providers participated in this study from December 2014 to March 2015. Medical simulation software was used to simulate a patient's vital signs. We evaluated participants' ability to diagnose and treat vasovagal syncope or anaphylaxis with an evaluation sheet and conducted a questionnaire before and after the scenario-based simulation training. Results: The median evaluation sheet score for vasovagal syncope increased significantly from 7/9 before to 9/9 after simulation training. The median score for anaphylaxis also increased significantly from 8/12 to 12/12 (P &lt
.01). For the item “I can treat vasovagal syncope/anaphylaxis adequately,” the percentage responding “Strongly agree” or “Agree” increased from 14% to 56% for vasovagal syncope and from 6% to 42% for anaphylaxis with simulation training. Conclusions: This simulation course improved participants' ability to diagnose and treat medical emergencies and improved their confidence. This course can be offered inexpensively using a software application.

DOI： 10.1111/eje.12301

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Language：English  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Netherlands  

Elderly people whose daily activities have declined due to a cerebrovascular disorder may suffer from dysphagia and may find oral hygiene difficult. Therefore, it is important to establish an effective therapy for the underlying cerebrovascular disorder. Dedifferentiated fat cells (DFAT) were obtained from mature adipocytes isolated from human buccal adipose pads in a ceiling culture. DFAT expressed the neural markers Nestin and SOX2. Flow cytometric analysis revealed that the cells had properties similar to mesenchymal stem cells. Although the transplantation of DFAT did not change the infarction area and volume ratios in a murine cerebral infarction model, functional recovery was observed in behavioral tests. Furthermore, DFAT administered to mice were later detected in cerebral infarctions. It therefore appears that transplanted DFAT affect the brain after infarction and contribute to the promotion of functional recovery. This finding may provide new cell replacement therapy options for treating disorders of the central nervous system.

DOI： 10.1007/s10616-018-0193-9

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Language：English  

DOI： 10.1111/odi.12832

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Language：English  

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Language：English   Publisher：Association for Dental Sciences of the Republic of China  

DOI： 10.1016/j.jds.2016.02.005

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DOI： 10.3892/mco.2017.1242

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

Purpose Human dedifferentiated fat (hDFAT) cells are thought to be a promising cell source for cartilage regeneration therapy. Nevertheless, the responses of hDFAT cells to bone morphogenetic proteins (BMPs) are still unclear. Here, we elucidated the effects of BMP-4 on the mRNA expression of early chondrogenic markers in hDFAT cells under monolayer or pellet cell culture conditions. Materials and methods Monolayer and pellet cell cultures of hDFAT cells were grown with control medium or chondrogenic medium (CM) with or without BMP-2, BMP-4, or BMP-7. Real-time polymerase chain reaction was used to analyze the mRNA expression levels of nine genes: chondrogenic markers, i.e., SOX9, SOX5, SOX6, aggrecan, type 2 collagen, type 10 collagen, and matrix metalloproteinase (MMP) 13
type 1 collagen
and MMP3. The BMP signaling inhibitor dorsomorphin was used to verify the mechanisms of BMP-4-induced chondrogenesis. Results Recombinant BMP-4 (100 ng/mL) increased the expression of SOX9, SOX6, and aggrecan mRNAs in monolayer cells compared with that in cells treated with BMP-2 or BMP-7 on day 3. Chondrogenically differentiated hDFAT cells induced by CM containing BMP-4 showed higher expression of eight genes (excluding SOX5) in monolayer cultures and nine genes (including SOX5) in pellet cultures compared with those in control medium on day 14. Dorsomorphin attenuated the effects of BMP-4. Conclusion These results showed that BMP-4 had the potential to modulate the early chondrogenesis of hDFAT cells under both monolayer and pellet cell culture conditions.

DOI： 10.1016/j.odw.2016.10.006

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Language：English   Publisher：Osaka Odontological Society  

Lidocaine hydrochloride and alkalinized lidocaine hydrochloride solutions were filled in en­ dotracheal tube cuffs to determine the rate of diffusion of lidocaine across the cuffs, and assess the usefulness of these cuffs as a drug delivery system. Mallinckrodt^TMOral RAE^®tracheal tubes were filled with three different lidocaine solutions, i.e., mixtures of 4% lido­ caine hydrochloride solution and distilled water, 4% lidocaine hydrochloride solution an 8.4% sodium bicarbonate solution (LSB­Gr), and 4% lidocaine hydrochloride solution and dipotassium phosphate solution (LDP­Gr). Cuffs filled with the relevant lidocaine solution were placed in beakers filled with distilled water. A 100 μL sample of the water in the vessel was taken from each beaker every 30 minutes for 360 minutes to determine the concentra­ tion of lidocaine diffused across the cuff using fluorescence polarization immunoassay.The cuff surface was observed after 60, 180, and 360 minutes of exposure for changes in the structure of the material. Lidocaine in LSB­Gr and LDP­Gr diffused across the cuffs at 30 minutes of exposure and thereafter. The lidocaine concentration in water in the vessel was 133.8 μg/mL for LSB­Gr, and 119.0 μg/mL for LDP­Gr. Although the cuffs did not rup­ ture during exposure, the cuff material deteriorated over time. The results indicate that alka­ linization of intracuff lidocaine increases the rate of diffusion of lidocaine across the en­ dotracheal tube cuff and affects the cuff material, which increases the risk of complications due to cuff rupture. (J Osaka Dent Univ 2016 ;50 : 1−6)

DOI： 10.18905/jodu.50.1_1

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Authorship：Lead author   Language：English   Publisher：ELSEVIER SCIENCE INC  

DOI： 10.1016/j.jclinane.2015.10.004

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：MARY ANN LIEBERT, INC  

Dedifferentiated fat cells (DFAT cells) isolated from adipose tissue have been demonstrated to differentiate into chondrogenic cells in vitro. Nevertheless, an efficient method to facilitate its chondrogenic differentiation is still unexplored, hampering the extensive application of these cells in cartilage regeneration therapies. Here we provide the evidence that supplementation of strontium ions (Sr) in a chondrogenic medium (CM) significantly promotes early chondrogenic differentiation of DFAT cells. Human DFAT cells and the mesenchymal stem cell line (RCB2153) were subjected to the CM supplemented with/without Sr. After 14 days, alcian blue staining intensity significantly increased in DFAT cells, but not in RCB2153, subjected to CM with Sr. mRNA expression analysis revealed that the CM with 1.5 mM Sr increased the expression of chondrogenic marker, collagen type 2 alpha 1, whereas there was no significant change in osteogenic markers, collagen type 1 alpha 1, runt-related transcription factor 2, and osteocalcin, and hypertrophic chondrogenic marker, collagen type 10 alpha 1. Inhibitors for extracellular signal-regulated kinase 1/2 (ERK1/2), Akt, and calcium-sensing receptor (CaSR) pathways significantly diminished the alcian blue staining intensity, providing the first evidence that these signal pathways are associated with chondrogenic differentiation of DFAT cells. CaSR and ERK1/2 pathways independently induced Sr-mediated early chondrogenic differentiation. These results suggest that Sr supplementation into the CM may provide a powerful platform for preparing chondrogenically differentiated DFAT cells for cartilage regeneration.

DOI： 10.1089/ten.tea.2014.0282

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DOI： 10.1002/ccr3.197

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

We compared the osteoblastic differentiation abilities of dedifferentiated fat cells (DFATs) and human bone marrow mesenchymal stem cells (hMSCs) as a cell source for bone regeneration therapies. In addition, the utility of DFATs in bone tissue engineering in vitro was assessed by an alpha-tricalcium phosphate (alpha-TCP)/collagen sponge (CS). Human DFATs were isolated from the submandibular of a patient by ceiling culture. DFATs and hMSCs at passage 3 were cultured in control medium or osteogenic medium (OM) for 14 days. Runx2 gene expression, alkaline phosphatase (ALP) activity, as well as osteocalcin (OCN) and calcium contents were analyzed to evaluate the osteoblastic differentiation ability of both cell types. DFATs seeded in a alpha-TCP/CS and cultured in OM for 14 days were analyzed by scanning electron microscopy (SEM) and histologically. Compared with hMSCs, DFATs cultured in OM generally underwent superior osteoblastogenesis by higher Runx2 gene expression at all days tested, as well as higher ALP activity at day 3 and 7, OCN expression at day 14, and calcium content at day 7. In SEM analyses, DFATs seeded in a alpha-TCP/CS were well spread and covered the alpha-TCP/CS by day 7. In addition, numerous spherical deposits were found to almost completely cover the alpha-TCP/CS on day 14. Von Kossa staining showed that DFATs differentiated into osteoblasts in the alpha-TCP/CS and formed cultured bone by deposition of a mineralized extracellular matrix. The combined use of DFATs and an alpha-TCP/CS may be an attractive option for bone tissue engineering.

DOI： 10.1007/s10616-013-9659-y

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Language：English   Publisher：Osaka Odontological Society  

We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat pad (BFP). We isolated the cells from two patients who underwent oral and maxillofacial surgery. Chondrocyte differentiation was evaluated based on gene and protein expression analysis in addition to histological analysis of DFATs and ASCs seeded in an atelocollagen sponge and cultured for up to 21 days. The gene expression levels of chondrocyte differentiation markers such as aggrecan, collagen type 2, and SOX9 were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested. Additionally, the levels of the embryonic stem cell markers Nanog, SOX2, and OCT4 were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcian blue-positive, indicating production of sulfated glycosaminoglycans, and was aggrecan-positive.<br> The chondrocyte differentiation ability of human DFATs was higher than that of ASCs. Isolation of DFATs from the BFP offers an aesthetic advantage, as the BFP can be obtained from the oral cavity without surface scarring. Therefore, we propose that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering. This study provides evidence that DFATs from the BFP are an ideal cell source for cartilage tissue engineering.

DOI： 10.18905/jodu.49.2_185

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER HEIDELBERG  

The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP).
We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types.
ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures.
The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP.
Isolation of DFAT cells from the BFP has an esthetic advantage because the BFP can be obtained via the oral cavity without injury to the external body surface. Therefore, we consider that DFAT cells from the BFP are an ideal cell source for bone tissue engineering.

DOI： 10.1007/s00784-013-1166-1

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Association of Regenerative Dentistry  

Strontium (Sr) ions are widely applied in bone regeneration therapy. However, it remains unclear whether Sr has the capacity for application in cartilage regeneration therapy using adipose-derived stem cells (ADSCs). Here, we demonstrate that the Sr ions contained in chondrogenic medium (CM) potently enhanced the chondrogenic differentiation of human ADSCs <i>in vitro</i>. Human ADSCs were isolated from the buccal fat pad of a middle-aged woman during oral surgery. ADSCs were then exposed to CM with or without Sr (0.15–15.00 mM) for up to 14 days. CM containing 1.50 mM Sr significantly increased the secretion of glycosaminoglycans (GAGs) and the mRNA expression of SRY(sex determining region Y)-box 9 and collagen type 2 alpha 1 compared with CM alone and basal medium. There were no obvious increases in adipogenic, osteoblastic, or hypertrophic chondrogenic differentiation markers. The results indicated that Sr was prospective agent to induce the chondrogenic differentiation of ADSCs.

DOI： 10.11223/jarde.12.27

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Language：English   Publisher：ELSEVIER TAIWAN  

DOI： 10.1016/j.jds.2012.12.003

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER  

Mature adipocyte-derived dedifferentiated fat (DFAT) cells rapidly differentiate into osteoblasts under three-dimensional culture conditions. However, it has not been demonstrated that DFAT cells can differentiate into osteoblasts in a rigid scaffold consisting of titanium fiber mesh (TFM). We examined the proliferation and osteogenic differentiation ability of DFAT cells using TFM as a scaffold. DFAT cells derived from rabbit subcutaneous fat were seeded into TFM and cultured in osteogenic medium containing dexamethasone, l-ascorbic acid 2-phosphate and beta-glycerophosphate for 14 days. In scanning electron microscopy (SEM) analysis, well-spread cells covered the titanium fibers on day 3, and appeared to increase in number from day 3 to 7. Numerous globular accretions were found and almost completely covered the fibers on day 14. Cell proliferation, as measured by DNA content in the TFM, was significantly higher on day 7 compared with that of day 1. Osteocalcin and calcium content in the TFM were significantly higher on day 14 compared to those of days 1, 3, and 7, indicating DFAT cells differentiated into osteoblasts. We theorize that globular accretions observed in SEM analysis may be calcified matrix resulting from osteocalcin secreted by osteoblasts binding calcium contained in fetal bovine serum. In this study, we demonstrated that DFAT cells differentiate into osteoblasts and deposit mineralized matrices in TFM. Therefore, the combination of DFAT cells and TFM may be an attractive option for bone tissue engineering.

DOI： 10.1007/s10616-012-9456-z

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Language：English   Publisher：Osaka Odontological Society  

DOI： 10.11223/jarde.8.151

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DOI： 10.2344/0003-3006-57.1.13

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER TOKYO  

We tested our hypothesis that a Parker-tipped tracheal tube could improve the reliability of tracheal tube passage as compared to a Murphy-tipped tracheal tube during Airway Scope-assisted intubation in a manikin.
Twenty-seven resident doctors performed Airway Scope-assisted intubations using both tracheal tubes, and the number of times the tracheal tube tip touched the glottis or other laryngeal structures was counted on the Airway Scope monitor.
The incidence of the tracheal tube tip touching the glottis during Airway Scope-assisted intubation using the Parker-tipped tracheal tube (7.4%; 2 of 27 attempts) was significantly less than that obtained using the Murphy-tipped tracheal tube (33.3%; 9 of 27 attempts; P = 0.039).
When the advancement of a conventional tracheal tube during Airway Scope-assisted intubation is likely to result in collision with the glottis or other laryngeal structures, the selection of a Parker-tipped tracheal tube is a better option.

DOI： 10.1007/s00540-009-0812-x

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：W B SAUNDERS CO-ELSEVIER INC  

Purpose: This study compared the Portex introducer, ivory type (PII) (Portex Tracheal Tube Introducer; SIMS Portex, Hythe, Kent, England), and Cook Airway Exchange Catheter (CAEC) (Cook, Letchworth, Hertfordshire, England) with regard to success rate of and time required for tracheal intubation by use of each device by oral and maxillofacial surgeons on a manikin.
Materials and Methods: The subjects comprised 17 oral and maxillofacial surgeons who intubated the trachea of the manikin with each device. The PII or CAEC was inserted into the trachea of the manikin and advanced 26 cm from the upper incisor, and the time required for intubation was measured. The subjects were then asked to rate the difficulty of intubation using a 10-cm visual analog scale.
Results: The mean intubation time, averaged over 3 attempts, was significantly shorter for the PII (7.92 +/- 3.15 seconds) than for the CAEC (11.44 +/- 3.98 seconds) (P &lt; .001). The rate of successful intubation with the PII (88.2%) was significantly greater than that with the CAEC (37.3%) (P &lt; .001). With regard to the overall difficulty of intubation, as rated by the 10-cm visual analog scale, the PII (median, 45 mm [10th-90th percentile, 32-53 mm]) was considered less difficult than the CAEC (median, 66 mm [10th-90th percentile, 52-84 mm]) (P &lt; .001).
Conclusions: intubation is faster, more Sure, and easier by use of the PII as a tube exchanger than with the CAEC. This may be particularly important for patients undergoing oral and maxillofacial surgery as a result of anatomic changes in the airway, because edema or hematoma in the tipper airway often leads to failed intubation. We conclude that the PII is useful as a tube exchanger. (C) 2009 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 67:1615-1618, 2009

DOI： 10.1016/j.joms.2008.09.017

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Language：English   Publisher：ELSEVIER IRELAND LTD  

DOI： 10.1016/j.resuscitation.2008.12.011

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DOI： 10.18905/jodu.43.1_57

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DOI： 10.18905/jodu.43.1_51

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Language：English   Publisher：Japanese Association of Regenerative Dentistry  

Dedifferentiated fat cells (DFATs) possess osteogenic potential making them a promising cell source for bone regeneration. PuraMatrix^<TM> (PM), a self-assembling peptide scaffold, produces a nanoscale environment for cells. We examined radiologically the osteogenic potential and application of DFATs with PM for bone regeneration in rabbit calvarial defect models. DFATs were obtained from ceiling culture, and subsequently cultured in normal media (NM) or osteogenic media (OM). Their in vitro osteogenic differentiation potential was assessed using alizarin red staining, and by measuring osteocalcin expression and calcium concentration. Eighteen 6-mm calvarial defects were randomly treated in vivo with osteo-induced DFATs+PM, DFATs+PM, and PM scaffold, or no treatment. After 8 weeks, calvaria were harvested and the degree of healing between treatment groups was radiologically compared. Osteocalcin expression in OM significantly increased at 7 and 14 days, as well as calcium concentration at 21 days. There was no difference in healing among the four groups radiologically. Rabbit DFATs efficiently differentiate into osteoblasts when cultured in OM. To demonstrate DFAT usefulness in vivo, progressively more sophisticated animal models must be developed.

DOI： 10.11223/jarde.6.127

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Authorship：Lead author   Publishing type：Book review, literature introduction, etc.  

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Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

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Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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