記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2344/anpr-63-16-65

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：American Dental Society of Anesthesiology (ADSA)  

DOI： 10.2344/23-00043

researchmap

その他リンク： https://anesthesiaprogress.kglmeridian.com/downloadpdf/journals/anpr/71/1/article-p42.xml

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

Abstract

Introduction

As the population ages and more patients experience medical emergencies during dental treatments, dentists must competently and confidently manage these situations. We developed a simulation training course for medical emergencies in the dental setting using an inexpensive vital sign simulation app for smartphones/tablets without the need for an expensive simulator. However, the duration for which this effect is maintained is unclear. This study was performed to evaluate the long‐term educational effect at 3, 6, and 12 months after taking the course.

Materials and Methods

Thirty‐nine dental residents participated in this course. Scenarios included vasovagal syncope, anaphylaxis, hyperventilation syndrome, and acute coronary syndrome, each of which the participants had to diagnose and treat. The participants were evaluated using a checklist for anaphylaxis diagnosis and treatment skills immediately after and 3, 6, and 12 months after the course. The participants were also surveyed about their confidence in diagnosing and treating these conditions by questionnaire before, immediately after, and 3, 6, and 12 months after the course.

Results

The checklist scores for anaphylaxis were significantly lower at 3, 6, and 12 months after the course than immediately after the course. The percentage of participants who provided a correct diagnosis and appropriate treatment for vasovagal syncope, hyperventilation syndrome, and acute coronary syndrome was lower at all reassessments than immediately after the course.

Conclusion

Because medical emergency management skills and confidence declined within 3 months, it would be useful to introduce a refresher course approximately 3 months after the initial course to maintain skills and confidence.

DOI： 10.1111/eje.12996

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

DOI： 10.7759/cureus.52320

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2344/anpr-70-03-07

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

OBJECTIVE: The impact of the COVID-19 pandemic on dental anesthesiologists has not been examined. This study aimed to determine how the COVID-19 pandemic affected Japanese dental anesthesiologists' professional lives. METHODS: An online questionnaire related to the effects of COVID-19 on dental anesthesiologists' professional lives was emailed to 351 board-certified dental anesthesiology specialists from the Japanese Dental Society of Anesthesiology. The endpoints of this study were changes in income and job satisfaction as a dental anesthesiologist from 2019 prior to the COVID-19 pandemic. RESULTS: A total of 141 dental anesthesiologists participated in the survey. Most respondents reported no change in income relative to 2019 for 2020 or 2021. Significantly more dental anesthesiologists reported income decreases relative to 2019 for 2020 (39%) vs 2021 (21.3%; P = .001). Very few dental anesthesiologists reported income increases relative to 2019 for 2020 (2.1%) vs 2021 (15.6%; P < .001). Job satisfaction as a dental anesthesiologist remained unchanged for all 3 years. CONCLUSION: Even though many Japanese dental anesthesiologist specialists lost income because of COVID-19, they maintained their job satisfaction.

DOI： 10.2344/930714

PubMed

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：American Dental Society of Anesthesiology (ADSA)  

DOI： 10.2344/anpr-70-03-02

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.jds.2022.08.018

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：Cureus, Inc.  

DOI： 10.7759/cureus.33733

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can cause loss or alteration of taste and smell as early symptoms or sequelae, but the detailed mechanism behind this phenomenon remains unclear. Here, we investigated whether the SARS-CoV-2 spike protein induces taste cell apoptosis and expression of the apoptosis-related cytokine TNF-α in male Sprague-Dawley rats. Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-fluorescein nick end labeling (TUNEL) assay results revealed a significantly higher apoptosis index for taste cells in the SARS-CoV-2 group than for those in the control group. An immunohistochemistry analysis indicated significantly more TNF-α-positive cells in the SARS-CoV-2 group compared with the control group. These data suggest that the SARS-CoV-2 spike protein promotes taste cell apoptosis and the release of apoptosis-related cytokine TNF-α, implicating its contribution to the taste malfunction caused by coronavirus disease 2019 (COVID-19).

DOI： 10.1016/j.jds.2022.08.016

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections cause loss or alteration of taste and smell as early symptoms and sequelae, but the detailed mechanism remains unclear. This study investigated whether coronavirus disease 2019-induced taste disorders are caused by direct effects on taste bud cells. SARS-CoV-2 recombinant spike and nucleocapsid proteins were applied to circumvallate papillae of male Sprague-Dawley rats. Immunohistochemistry and image analysis were used to compare the number of taste buds, and taste bud cells and area, together with confirmation of angiotensin-converting enzyme 2 (ACE2) expression. Immunohistochemical analysis revealed ACE2 expression in the taste buds of rat circumvallate papillae. Decreases in the number of taste buds, taste bud cells, and their area were observed at 12 days after application of SARS-CoV-2 recombinant spike and nucleocapsid proteins. These data suggest that SARS-CoV-2 proteins induce degeneration of taste buds.

DOI： 10.1016/j.jds.2022.03.009

PubMed

researchmap

担当区分：筆頭著者,　責任著者  

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.jds.2021.10.015

PubMed

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本歯科薬物療法学会  

researchmap

掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/jdd.12481

Scopus

researchmap

担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

DOI： 10.1002/ca.23724

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2344/anpr-66-04-04

PubMed

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.jds.2019.11.007

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）  

researchmap

記述言語：日本語   出版者・発行元：(一社)日本歯科麻酔学会  

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Blackwell Publishing Ltd  

Introduction: Every dental provider needs to be educated about medical emergencies to provide safe dental care. Simulation training is available with simulators such as advanced life support manikins and robot patients. However, the purchase and development costs of these simulators are high. We have developed a simulation training course on medical emergencies using an inexpensive software application. The purpose of this study was to evaluate the educational effectiveness of this course. Materials and Methods: Fifty-one dental providers participated in this study from December 2014 to March 2015. Medical simulation software was used to simulate a patient's vital signs. We evaluated participants' ability to diagnose and treat vasovagal syncope or anaphylaxis with an evaluation sheet and conducted a questionnaire before and after the scenario-based simulation training. Results: The median evaluation sheet score for vasovagal syncope increased significantly from 7/9 before to 9/9 after simulation training. The median score for anaphylaxis also increased significantly from 8/12 to 12/12 (P &lt
.01). For the item “I can treat vasovagal syncope/anaphylaxis adequately,” the percentage responding “Strongly agree” or “Agree” increased from 14% to 56% for vasovagal syncope and from 6% to 42% for anaphylaxis with simulation training. Conclusions: This simulation course improved participants' ability to diagnose and treat medical emergencies and improved their confidence. This course can be offered inexpensively using a software application.

DOI： 10.1111/eje.12301

Scopus

researchmap

記述言語：英語  

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Netherlands  

Elderly people whose daily activities have declined due to a cerebrovascular disorder may suffer from dysphagia and may find oral hygiene difficult. Therefore, it is important to establish an effective therapy for the underlying cerebrovascular disorder. Dedifferentiated fat cells (DFAT) were obtained from mature adipocytes isolated from human buccal adipose pads in a ceiling culture. DFAT expressed the neural markers Nestin and SOX2. Flow cytometric analysis revealed that the cells had properties similar to mesenchymal stem cells. Although the transplantation of DFAT did not change the infarction area and volume ratios in a murine cerebral infarction model, functional recovery was observed in behavioral tests. Furthermore, DFAT administered to mice were later detected in cerebral infarctions. It therefore appears that transplanted DFAT affect the brain after infarction and contribute to the promotion of functional recovery. This finding may provide new cell replacement therapy options for treating disorders of the central nervous system.

DOI： 10.1007/s10616-018-0193-9

Web of Science

Scopus

PubMed

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語  

DOI： 10.1111/odi.12832

PubMed

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語  

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   出版者・発行元：Association for Dental Sciences of the Republic of China  

DOI： 10.1016/j.jds.2016.02.005

Web of Science

Scopus

researchmap

記述言語：英語  

DOI： 10.3892/mco.2017.1242

PubMed

researchmap

担当区分：責任著者   記述言語：英語  

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語  

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier Ltd  

Purpose Human dedifferentiated fat (hDFAT) cells are thought to be a promising cell source for cartilage regeneration therapy. Nevertheless, the responses of hDFAT cells to bone morphogenetic proteins (BMPs) are still unclear. Here, we elucidated the effects of BMP-4 on the mRNA expression of early chondrogenic markers in hDFAT cells under monolayer or pellet cell culture conditions. Materials and methods Monolayer and pellet cell cultures of hDFAT cells were grown with control medium or chondrogenic medium (CM) with or without BMP-2, BMP-4, or BMP-7. Real-time polymerase chain reaction was used to analyze the mRNA expression levels of nine genes: chondrogenic markers, i.e., SOX9, SOX5, SOX6, aggrecan, type 2 collagen, type 10 collagen, and matrix metalloproteinase (MMP) 13
type 1 collagen
and MMP3. The BMP signaling inhibitor dorsomorphin was used to verify the mechanisms of BMP-4-induced chondrogenesis. Results Recombinant BMP-4 (100 ng/mL) increased the expression of SOX9, SOX6, and aggrecan mRNAs in monolayer cells compared with that in cells treated with BMP-2 or BMP-7 on day 3. Chondrogenically differentiated hDFAT cells induced by CM containing BMP-4 showed higher expression of eight genes (excluding SOX5) in monolayer cultures and nine genes (including SOX5) in pellet cultures compared with those in control medium on day 14. Dorsomorphin attenuated the effects of BMP-4. Conclusion These results showed that BMP-4 had the potential to modulate the early chondrogenesis of hDFAT cells under both monolayer and pellet cell culture conditions.

DOI： 10.1016/j.odw.2016.10.006

Web of Science

Scopus

researchmap

記述言語：英語   出版者・発行元：大阪歯科学会  

Lidocaine hydrochloride and alkalinized lidocaine hydrochloride solutions were filled in en­ dotracheal tube cuffs to determine the rate of diffusion of lidocaine across the cuffs, and assess the usefulness of these cuffs as a drug delivery system. Mallinckrodt^TMOral RAE^®tracheal tubes were filled with three different lidocaine solutions, i.e., mixtures of 4% lido­ caine hydrochloride solution and distilled water, 4% lidocaine hydrochloride solution an 8.4% sodium bicarbonate solution (LSB­Gr), and 4% lidocaine hydrochloride solution and dipotassium phosphate solution (LDP­Gr). Cuffs filled with the relevant lidocaine solution were placed in beakers filled with distilled water. A 100 μL sample of the water in the vessel was taken from each beaker every 30 minutes for 360 minutes to determine the concentra­ tion of lidocaine diffused across the cuff using fluorescence polarization immunoassay.The cuff surface was observed after 60, 180, and 360 minutes of exposure for changes in the structure of the material. Lidocaine in LSB­Gr and LDP­Gr diffused across the cuffs at 30 minutes of exposure and thereafter. The lidocaine concentration in water in the vessel was 133.8 μg/mL for LSB­Gr, and 119.0 μg/mL for LDP­Gr. Although the cuffs did not rup­ ture during exposure, the cuff material deteriorated over time. The results indicate that alka­ linization of intracuff lidocaine increases the rate of diffusion of lidocaine across the en­ dotracheal tube cuff and affects the cuff material, which increases the risk of complications due to cuff rupture. (J Osaka Dent Univ 2016 ;50 : 1−6)

DOI： 10.18905/jodu.50.1_1

CiNii Article

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   出版者・発行元：ELSEVIER SCIENCE INC  

DOI： 10.1016/j.jclinane.2015.10.004

Web of Science

PubMed

researchmap

担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

Dedifferentiated fat cells (DFAT cells) isolated from adipose tissue have been demonstrated to differentiate into chondrogenic cells in vitro. Nevertheless, an efficient method to facilitate its chondrogenic differentiation is still unexplored, hampering the extensive application of these cells in cartilage regeneration therapies. Here we provide the evidence that supplementation of strontium ions (Sr) in a chondrogenic medium (CM) significantly promotes early chondrogenic differentiation of DFAT cells. Human DFAT cells and the mesenchymal stem cell line (RCB2153) were subjected to the CM supplemented with/without Sr. After 14 days, alcian blue staining intensity significantly increased in DFAT cells, but not in RCB2153, subjected to CM with Sr. mRNA expression analysis revealed that the CM with 1.5 mM Sr increased the expression of chondrogenic marker, collagen type 2 alpha 1, whereas there was no significant change in osteogenic markers, collagen type 1 alpha 1, runt-related transcription factor 2, and osteocalcin, and hypertrophic chondrogenic marker, collagen type 10 alpha 1. Inhibitors for extracellular signal-regulated kinase 1/2 (ERK1/2), Akt, and calcium-sensing receptor (CaSR) pathways significantly diminished the alcian blue staining intensity, providing the first evidence that these signal pathways are associated with chondrogenic differentiation of DFAT cells. CaSR and ERK1/2 pathways independently induced Sr-mediated early chondrogenic differentiation. These results suggest that Sr supplementation into the CM may provide a powerful platform for preparing chondrogenically differentiated DFAT cells for cartilage regeneration.

DOI： 10.1089/ten.tea.2014.0282

Web of Science

PubMed

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語  

DOI： 10.1002/ccr3.197

Web of Science

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

We compared the osteoblastic differentiation abilities of dedifferentiated fat cells (DFATs) and human bone marrow mesenchymal stem cells (hMSCs) as a cell source for bone regeneration therapies. In addition, the utility of DFATs in bone tissue engineering in vitro was assessed by an alpha-tricalcium phosphate (alpha-TCP)/collagen sponge (CS). Human DFATs were isolated from the submandibular of a patient by ceiling culture. DFATs and hMSCs at passage 3 were cultured in control medium or osteogenic medium (OM) for 14 days. Runx2 gene expression, alkaline phosphatase (ALP) activity, as well as osteocalcin (OCN) and calcium contents were analyzed to evaluate the osteoblastic differentiation ability of both cell types. DFATs seeded in a alpha-TCP/CS and cultured in OM for 14 days were analyzed by scanning electron microscopy (SEM) and histologically. Compared with hMSCs, DFATs cultured in OM generally underwent superior osteoblastogenesis by higher Runx2 gene expression at all days tested, as well as higher ALP activity at day 3 and 7, OCN expression at day 14, and calcium content at day 7. In SEM analyses, DFATs seeded in a alpha-TCP/CS were well spread and covered the alpha-TCP/CS by day 7. In addition, numerous spherical deposits were found to almost completely cover the alpha-TCP/CS on day 14. Von Kossa staining showed that DFATs differentiated into osteoblasts in the alpha-TCP/CS and formed cultured bone by deposition of a mineralized extracellular matrix. The combined use of DFATs and an alpha-TCP/CS may be an attractive option for bone tissue engineering.

DOI： 10.1007/s10616-013-9659-y

Web of Science

PubMed

researchmap

記述言語：英語   出版者・発行元：大阪歯科学会  

We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat pad (BFP). We isolated the cells from two patients who underwent oral and maxillofacial surgery. Chondrocyte differentiation was evaluated based on gene and protein expression analysis in addition to histological analysis of DFATs and ASCs seeded in an atelocollagen sponge and cultured for up to 21 days. The gene expression levels of chondrocyte differentiation markers such as aggrecan, collagen type 2, and SOX9 were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested. Additionally, the levels of the embryonic stem cell markers Nanog, SOX2, and OCT4 were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcian blue-positive, indicating production of sulfated glycosaminoglycans, and was aggrecan-positive.<br> The chondrocyte differentiation ability of human DFATs was higher than that of ASCs. Isolation of DFATs from the BFP offers an aesthetic advantage, as the BFP can be obtained from the oral cavity without surface scarring. Therefore, we propose that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering. This study provides evidence that DFATs from the BFP are an ideal cell source for cartilage tissue engineering.

DOI： 10.18905/jodu.49.2_185

CiNii Article

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER HEIDELBERG  

The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP).
We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types.
ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures.
The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP.
Isolation of DFAT cells from the BFP has an esthetic advantage because the BFP can be obtained via the oral cavity without injury to the external body surface. Therefore, we consider that DFAT cells from the BFP are an ideal cell source for bone tissue engineering.

DOI： 10.1007/s00784-013-1166-1

Web of Science

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japanese Association of Regenerative Dentistry  

Strontium (Sr) ions are widely applied in bone regeneration therapy. However, it remains unclear whether Sr has the capacity for application in cartilage regeneration therapy using adipose-derived stem cells (ADSCs). Here, we demonstrate that the Sr ions contained in chondrogenic medium (CM) potently enhanced the chondrogenic differentiation of human ADSCs <i>in vitro</i>. Human ADSCs were isolated from the buccal fat pad of a middle-aged woman during oral surgery. ADSCs were then exposed to CM with or without Sr (0.15–15.00 mM) for up to 14 days. CM containing 1.50 mM Sr significantly increased the secretion of glycosaminoglycans (GAGs) and the mRNA expression of SRY(sex determining region Y)-box 9 and collagen type 2 alpha 1 compared with CM alone and basal medium. There were no obvious increases in adipogenic, osteoblastic, or hypertrophic chondrogenic differentiation markers. The results indicated that Sr was prospective agent to induce the chondrogenic differentiation of ADSCs.

DOI： 10.11223/jarde.12.27

CiNii Article

researchmap

記述言語：英語   出版者・発行元：ELSEVIER TAIWAN  

DOI： 10.1016/j.jds.2012.12.003

Web of Science

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

Mature adipocyte-derived dedifferentiated fat (DFAT) cells rapidly differentiate into osteoblasts under three-dimensional culture conditions. However, it has not been demonstrated that DFAT cells can differentiate into osteoblasts in a rigid scaffold consisting of titanium fiber mesh (TFM). We examined the proliferation and osteogenic differentiation ability of DFAT cells using TFM as a scaffold. DFAT cells derived from rabbit subcutaneous fat were seeded into TFM and cultured in osteogenic medium containing dexamethasone, l-ascorbic acid 2-phosphate and beta-glycerophosphate for 14 days. In scanning electron microscopy (SEM) analysis, well-spread cells covered the titanium fibers on day 3, and appeared to increase in number from day 3 to 7. Numerous globular accretions were found and almost completely covered the fibers on day 14. Cell proliferation, as measured by DNA content in the TFM, was significantly higher on day 7 compared with that of day 1. Osteocalcin and calcium content in the TFM were significantly higher on day 14 compared to those of days 1, 3, and 7, indicating DFAT cells differentiated into osteoblasts. We theorize that globular accretions observed in SEM analysis may be calcified matrix resulting from osteocalcin secreted by osteoblasts binding calcium contained in fetal bovine serum. In this study, we demonstrated that DFAT cells differentiate into osteoblasts and deposit mineralized matrices in TFM. Therefore, the combination of DFAT cells and TFM may be an attractive option for bone tissue engineering.

DOI： 10.1007/s10616-012-9456-z

Web of Science

PubMed

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   出版者・発行元：大阪歯科学会  

DOI： 10.11223/jarde.8.151

CiNii Article

researchmap

記述言語：英語  

DOI： 10.2344/0003-3006-57.1.13

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER TOKYO  

We tested our hypothesis that a Parker-tipped tracheal tube could improve the reliability of tracheal tube passage as compared to a Murphy-tipped tracheal tube during Airway Scope-assisted intubation in a manikin.
Twenty-seven resident doctors performed Airway Scope-assisted intubations using both tracheal tubes, and the number of times the tracheal tube tip touched the glottis or other laryngeal structures was counted on the Airway Scope monitor.
The incidence of the tracheal tube tip touching the glottis during Airway Scope-assisted intubation using the Parker-tipped tracheal tube (7.4%; 2 of 27 attempts) was significantly less than that obtained using the Murphy-tipped tracheal tube (33.3%; 9 of 27 attempts; P = 0.039).
When the advancement of a conventional tracheal tube during Airway Scope-assisted intubation is likely to result in collision with the glottis or other laryngeal structures, the selection of a Parker-tipped tracheal tube is a better option.

DOI： 10.1007/s00540-009-0812-x

Web of Science

PubMed

researchmap

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：W B SAUNDERS CO-ELSEVIER INC  

Purpose: This study compared the Portex introducer, ivory type (PII) (Portex Tracheal Tube Introducer; SIMS Portex, Hythe, Kent, England), and Cook Airway Exchange Catheter (CAEC) (Cook, Letchworth, Hertfordshire, England) with regard to success rate of and time required for tracheal intubation by use of each device by oral and maxillofacial surgeons on a manikin.
Materials and Methods: The subjects comprised 17 oral and maxillofacial surgeons who intubated the trachea of the manikin with each device. The PII or CAEC was inserted into the trachea of the manikin and advanced 26 cm from the upper incisor, and the time required for intubation was measured. The subjects were then asked to rate the difficulty of intubation using a 10-cm visual analog scale.
Results: The mean intubation time, averaged over 3 attempts, was significantly shorter for the PII (7.92 +/- 3.15 seconds) than for the CAEC (11.44 +/- 3.98 seconds) (P &lt; .001). The rate of successful intubation with the PII (88.2%) was significantly greater than that with the CAEC (37.3%) (P &lt; .001). With regard to the overall difficulty of intubation, as rated by the 10-cm visual analog scale, the PII (median, 45 mm [10th-90th percentile, 32-53 mm]) was considered less difficult than the CAEC (median, 66 mm [10th-90th percentile, 52-84 mm]) (P &lt; .001).
Conclusions: intubation is faster, more Sure, and easier by use of the PII as a tube exchanger than with the CAEC. This may be particularly important for patients undergoing oral and maxillofacial surgery as a result of anatomic changes in the airway, because edema or hematoma in the tipper airway often leads to failed intubation. We conclude that the PII is useful as a tube exchanger. (C) 2009 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 67:1615-1618, 2009

DOI： 10.1016/j.joms.2008.09.017

Web of Science

PubMed

researchmap

記述言語：英語   出版者・発行元：ELSEVIER IRELAND LTD  

DOI： 10.1016/j.resuscitation.2008.12.011

Web of Science

PubMed

researchmap

記述言語：英語  

DOI： 10.18905/jodu.43.1_51

researchmap

記述言語：英語  

DOI： 10.18905/jodu.43.1_57

researchmap

記述言語：英語  

CiNii Article

CiNii Books

researchmap

担当区分：筆頭著者,　責任著者   記述言語：英語   出版者・発行元：日本再生歯科医学会  

Dedifferentiated fat cells (DFATs) possess osteogenic potential making them a promising cell source for bone regeneration. PuraMatrix^<TM> (PM), a self-assembling peptide scaffold, produces a nanoscale environment for cells. We examined radiologically the osteogenic potential and application of DFATs with PM for bone regeneration in rabbit calvarial defect models. DFATs were obtained from ceiling culture, and subsequently cultured in normal media (NM) or osteogenic media (OM). Their in vitro osteogenic differentiation potential was assessed using alizarin red staining, and by measuring osteocalcin expression and calcium concentration. Eighteen 6-mm calvarial defects were randomly treated in vivo with osteo-induced DFATs+PM, DFATs+PM, and PM scaffold, or no treatment. After 8 weeks, calvaria were harvested and the degree of healing between treatment groups was radiologically compared. Osteocalcin expression in OM significantly increased at 7 and 14 days, as well as calcium concentration at 21 days. There was no difference in healing among the four groups radiologically. Rabbit DFATs efficiently differentiate into osteoblasts when cultured in OM. To demonstrate DFAT usefulness in vivo, progressively more sophisticated animal models must be developed.

DOI： 10.11223/jarde.6.127

CiNii Article

CiNii Books

researchmap

researchmap

researchmap

担当区分：筆頭著者   掲載種別：書評論文，書評，文献紹介等  

researchmap

担当区分：筆頭著者   掲載種別：書評論文，書評，文献紹介等  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

会議種別：シンポジウム・ワークショップ パネル（指名）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

会議種別：口頭発表（招待・特別）  

researchmap

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

researchmap

記述言語：日本語   会議種別：口頭発表（招待・特別）  

researchmap

記述言語：日本語   会議種別：シンポジウム・ワークショップ パネル（指名）  

researchmap

記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

researchmap

記述言語：英語   会議種別：口頭発表（招待・特別）  

researchmap

記述言語：日本語   会議種別：口頭発表（招待・特別）  

researchmap

researchmap