Updated on 2025/09/18

写真a

 
UCHIGASHIMA Motokazu
 
Organization
Brain Research Institute Associate Professor
. Research Professor
Title
Associate Professor
External link

Degree

  • 博士（医学） ( 2011.3   北海道大学 )

Research Interests

  • 分子イメージング

  • 包括脳ネットワーク

  • Monoamine

  • Striatum

  • Endocannabinoid

  • Neurotransmission

  • Synaptic cell adhesion molecules

Research Areas

  • Life Science / Anatomy and histopathology of nervous system

  • Life Science / Neuroscience-general

Research History (researchmap)

  • Niigata University   研究教授

    2022.11

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  • International Research Center for Neurointelligence, The University of Tokyo Institutes for Advanced Study   Affiliated Faculty

    2021.10

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  • Niigata University   Brain Research Institute   Associate Professor

    2019.3

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  • Hokkaido University   Assistant Professor

    2017.4 - 2019.2

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  • University of Massachusetts Medical School   Brudnick Neuropsychiatric Research Institute   Visiting Scholar

    2016.2 - 2018.3

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  • Hokkaido University   Graduate School of Medicine   Assistant Professor

    2011.4 - 2017.3

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  • 日本学術振興会特別研究員   DC1

    2008.4 - 2011.3

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  • Hokkaido University   Graduate School of Medicine

    2007.4 - 2011.3

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  • Hokkaido University   School of Medicine

    2001.4 - 2007.3

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Research History

  • Niigata University   Brain Research Institute Basic Neuroscience Branch Department of Cellular Neuropathology   Associate Professor

    2019.3

 

Papers

  • SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging. Reviewed International journal

    Xinyi Liu, Motokazu Uchigashima, Ikumi Oomoto, Yoshihito Saito, Hitoshi Uchida, Shinya Oginezawa, Keiko Masuda, Daisuke Satoh, Manabu Abe, Kenji Sakimura, Yoshihiro Shimizu, Masanori Murayama, Kazuki Tainaka, Takayasu Mikuni

    Nature communications   16 ( 1 )   7584 - 7584   2025.8

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Light scattering in the skull limits optical access to the brain. Here we present SeeThrough, a skull-clearing technique that enables simple, high-resolution, and minimally-invasive brain imaging without skull removal. Through systematic screening of over 1600 chemicals, we rationally developed a refractive index-matching solution that combines water- and organic solvent-based components, achieving both high clearing efficiency and biocompatibility. The reagents exhibit minimal brain penetration, maintain tissue integrity, and avoid inflammatory responses. Notably, SeeThrough provides imaging sensitivity and contrast comparable to open-skull window imaging, while permitting minimally-invasive monitoring of brain border macrophages as well as blood and cerebrospinal fluid dynamics. Combined with two-photon imaging, SeeThrough enables spatially and temporally scalable imaging applications in the mouse brain, including ~400 µm deep imaging, one-month longitudinal imaging, and mesoscale, cellular-resolution monitoring of brain activity for network-level analysis. Thus, SeeThrough offers a broadly accessible platform for high-throughput, physiology-preserving imaging of the brain parenchyma and brain-skull interface.

    DOI： 10.1038/s41467-025-62836-1

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  • Single-cell synaptome mapping: its technical basis and applications in critical period plasticity research Reviewed International journal

    Motokazu Uchigashima, Takayasu Mikuni

    Front Neural Circuits   18   1523614 - 1523614   2024

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Our brain adapts to the environment by optimizing its function through experience-dependent cortical plasticity. This plasticity is transiently enhanced during a developmental stage, known as the "critical period," and subsequently maintained at lower levels throughout adulthood. Thus, understanding the mechanism underlying critical period plasticity is crucial for improving brain adaptability across the lifespan. Critical period plasticity relies on activity-dependent circuit remodeling through anatomical and functional changes at individual synapses. However, it remains challenging to identify the molecular signatures of synapses responsible for critical period plasticity and to understand how these plasticity-related synapses are spatiotemporally organized within a neuron. Recent advances in genetic tools and genome editing methodologies have enabled single-cell endogenous protein labeling in the brain, allowing for comprehensive molecular profiling of individual synapses within a neuron, namely "single-cell synaptome mapping." This promising approach can facilitate insights into the spatiotemporal organization of synapses that are sparse yet functionally important within single neurons. In this review, we introduce the basics of single-cell synaptome mapping and discuss its methodologies and applications to investigate the synaptic and cellular mechanisms underlying circuit remodeling during the critical period.

    DOI： 10.3389/fncir.2024.1523614

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  • Neuroligin-3: A Circuit-Specific Synapse Organizer That Shapes Normal Function and Autism Spectrum Disorder-Associated Dysfunction Invited Reviewed International journal

    Motokazu Uchigashima, Amy Cheung, Kensuke Futai

    Frontiers in Molecular Neuroscience   14   749164 - 749164   2021.10

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Chemical synapses provide a vital foundation for neuron-neuron communication and overall brain function. By tethering closely apposed molecular machinery for presynaptic neurotransmitter release and postsynaptic signal transduction, circuit- and context- specific synaptic properties can drive neuronal computations for animal behavior. Trans-synaptic signaling via synaptic cell adhesion molecules (CAMs) serves as a promising mechanism to generate the molecular diversity of chemical synapses. Neuroligins (Nlgns) were discovered as postsynaptic CAMs that can bind to presynaptic CAMs like Neurexins (Nrxns) at the synaptic cleft. Among the four (Nlgn1-4) or five (Nlgn1-3, Nlgn4X, and Nlgn4Y) isoforms in rodents or humans, respectively, Nlgn3 has a heterogeneous expression and function at particular subsets of chemical synapses and strong association with non-syndromic autism spectrum disorder (ASD). Several lines of evidence have suggested that the unique expression and function of Nlgn3 protein underlie circuit-specific dysfunction characteristic of non-syndromic ASD caused by the disruption of Nlgn3 gene. Furthermore, recent studies have uncovered the molecular mechanism underlying input cell-dependent expression of Nlgn3 protein at hippocampal inhibitory synapses, in which trans-synaptic signaling of specific alternatively spliced isoforms of Nlgn3 and Nrxn plays a critical role. In this review article, we overview the molecular, anatomical, and physiological knowledge about Nlgn3, focusing on the circuit-specific function of mammalian Nlgn3 and its underlying molecular mechanism. This will provide not only new insight into specific Nlgn3-mediated trans-synaptic interactions as molecular codes for synapse specification but also a better understanding of the pathophysiological basis for non-syndromic ASD associated with functional impairment in Nlgn3 gene.

    DOI： 10.3389/fnmol.2021.749164

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  • Specific Neuroligin3-αNeurexin1 signaling regulates GABAergic synaptic function in mouse hippocampus. Reviewed International journal

    Motokazu Uchigashima, Kohtarou Konno, Emily Demchak, Amy Cheung, Takuya Watanabe, David G Keener, Manabu Abe, Timmy Le, Kenji Sakimura, Toshikuni Sasaoka, Takeshi Uemura, Yuka Imamura Kawasawa, Masahiko Watanabe, Kensuke Futai

    eLife   9   2020.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Synapse formation and regulation require signaling interactions between pre- and postsynaptic proteins, notably cell adhesion molecules (CAMs). It has been proposed that the functions of neuroligins (Nlgns), postsynaptic CAMs, rely on the formation of trans-synaptic complexes with neurexins (Nrxns), presynaptic CAMs. Nlgn3 is a unique Nlgn isoform that localizes at both excitatory and inhibitory synapses. However, Nlgn3 function mediated via Nrxn interactions is unknown. Here we demonstrate that Nlgn3 localizes at postsynaptic sites apposing vesicular glutamate transporter 3-expressing (VGT3+) inhibitory terminals and regulates VGT3+ inhibitory interneuron-mediated synaptic transmission in mouse organotypic slice cultures. Gene expression analysis of interneurons revealed that the αNrxn1+AS4 splice isoform is highly expressed in VGT3+ interneurons as compared with other interneurons. Most importantly, postsynaptic Nlgn3 requires presynaptic αNrxn1+AS4 expressed in VGT3+ interneurons to regulate inhibitory synaptic transmission. Our results indicate that specific Nlgn-Nrxn signaling generates distinct functional properties at synapses.

    DOI： 10.7554/eLife.59545

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  • Differential expression of neurexin genes in the mouse brain. Reviewed International journal

    Motokazu Uchigashima, Amy Cheung, Julie Suh, Masahiko Watanabe, Kensuke Futai

    The Journal of comparative neurology   527 ( 12 )   1940 - 1965   2019.8

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Synapses, highly specialized membrane junctions between neurons, connect presynaptic neurotransmitter release sites and postsynaptic ligand-gated channels. Neurexins (Nrxns), a family of presynaptic adhesion molecules, have been characterized as major regulators of synapse development and function. Via their extracellular domains, Nrxns bind to different postsynaptic proteins, generating highly diverse functional readouts through their postsynaptic binding partners. Not surprisingly given these versatile protein interactions, mutations and deletions of Nrxn genes have been identified in patients with autism spectrum disorders, intellectual disabilities, and schizophrenia. Therefore, elucidating the expression profiles of Nrxns in the brain is of high significance. Here, using chromogenic and fluorescent in situ hybridization, we characterize the expression patterns of Nrxn isoforms throughout the brain. We found that each Nrxn isoform displays a unique expression profile in a region-, cell type-, and sensory system-specific manner. Interestingly, we also found that αNrxn1 and αNrxn2 mRNAs are expressed in non-neuronal cells, including astrocytes and oligodendrocytes. Lastly, we found diverse expression patterns of genes that encode Nrxn binding proteins, such as Neuroligins (Nlgns), Leucine-rich repeat transmembrane neuronal protein (Lrrtms) and Latrophilins (Adgrls), suggesting that Nrxn proteins can mediate numerous combinations of trans-synaptic interactions. Together, our anatomical profiling of Nrxn gene expression reflects the diverse roles of Nrxn molecules.

    DOI： 10.1002/cne.24664

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  • Dopamine synapse is a neuroligin-2-mediated contact between dopaminergic presynaptic and GABAergic postsynaptic structures Reviewed

    Motokazu Uchigashima, Toshihisa Ohtsuka, Kazuto Kobayashi, Masahiko Watanabe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 15 )   4206 - 4211   2016.4

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    DOI： 10.1073/pnas.1514074113

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  • Single-cell endogenous protein labeling via CRISPR-Cas9-mediated genome editing in the mouse brain. Invited Reviewed

    Motokazu Uchigashima, Takayasu Mikuni

    Anatomical science international   2025.7

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    High-precision mapping of endogenous proteins is essential for understanding the molecular mechanism underlying neuronal functions in the brain. The SLENDR (single-cell labeling of endogenous proteins by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-mediated homology-directed repair) technique provides single-cell endogenous protein labeling with genetically encoded tags within the mammalian brain through precise genome editing via homology-directed repair (HDR). This technique is based on the introduction of HDR-mediated genome editing into neuronal progenitors in embryonic brains by in utero electroporation. Subsequent histological analyses enable high-resolution interrogation of the subcellular distribution of endogenous proteins within a single neuron using conventional fluorescent microscopy. Here, we describe a step-by-step protocol for the SLENDR technique to label endogenous proteins with genetically encoded tags in single pyramidal cells of the mouse primary somatosensory cortex. This protocol would be helpful to visualize the molecular organization underlying biological processes at single-neuron levels in the brain, such as signal processing from synaptic inputs to neuronal outputs across different scales.

    DOI： 10.1007/s12565-025-00866-x

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  • Regulation of Presynaptic Release Machinery by Cell Adhesion Molecules International journal

    Motokazu Uchigashima, Yasunori Hayashi, Kensuke Futai

    Advances in Neurobiology   33   333 - 356   2023

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    Authorship：Lead author   Language：English   Publishing type：Part of collection (book)  

    DOI： 10.1007/978-3-031-34229-5_13

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  • General Design Strategy to Precisely Control the Emission of Fluorophores via a Twisted Intramolecular Charge Transfer (TICT) Process. Reviewed International journal

    Kenjiro Hanaoka, Shimpei Iwaki, Kiyoshi Yagi, Takuya Myochin, Takayuki Ikeno, Hisashi Ohno, Eita Sasaki, Toru Komatsu, Tasuku Ueno, Motokazu Uchigashima, Takayasu Mikuni, Kazuki Tainaka, Shinya Tahara, Satoshi Takeuchi, Tahei Tahara, Masanobu Uchiyama, Tetsuo Nagano, Yasuteru Urano

    Journal of the American Chemical Society   144 ( 43 )   19778 - 19790   2022.10

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    Fluorogenic probes for bioimaging have become essential tools for life science and medicine, and the key to their development is a precise understanding of the mechanisms available for fluorescence off/on control, such as photoinduced electron transfer (PeT) and Förster resonance energy transfer (FRET). Here we establish a new molecular design strategy to rationally develop activatable fluorescent probes, which exhibit a fluorescence off/on change in response to target biomolecules, by controlling the twisted intramolecular charge transfer (TICT) process. This approach was developed on the basis of a thorough investigation of the fluorescence quenching mechanism of N-phenyl rhodamine dyes (commercially available as the QSY series) by means of time-dependent density functional theory (TD-DFT) calculations and photophysical evaluation of their derivatives. To illustrate and validate this TICT-based design strategy, we employed it to develop practical fluorogenic probes for HaloTag and SNAP-tag. We further show that the TICT-controlled fluorescence off/on mechanism is generalizable by synthesizing a Si-rhodamine-based fluorogenic probe for HaloTag, thus providing a palette of chemical dyes that spans the visible and near-infrared range.

    DOI： 10.1021/jacs.2c06397

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  • Enhanced Retrieval of Taste Associative Memory by Chemogenetic Activation of Locus Coeruleus Norepinephrine Neurons. Reviewed International journal

    Ryoji Fukabori, Yoshio Iguchi, Shigeki Kato, Kazumi Takahashi, Satoshi Eifuku, Shingo Tsuji, Akihiro Hazama, Motokazu Uchigashima, Masahiko Watanabe, Hiroshi Mizuma, Yilong Cui, Hirotaka Onoe, Keigo Hikishima, Yasunobu Yasoshima, Makoto Osanai, Ryo Inagaki, Kohji Fukunaga, Takuma Nishijo, Toshihiko Momiyama, Richard Benton, Kazuto Kobayashi

    The Journal of neuroscience : the official journal of the Society for Neuroscience   40 ( 43 )   8367 - 8385   2020.10

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    The ability of animals to retrieve memories stored in response to the environment is essential for behavioral adaptation. Norepinephrine (NE)-containing neurons in the brain play a key role in the modulation of synaptic plasticity underlying various processes of memory formation. However, the role of the central NE system in memory retrieval remains unclear. Here, we developed a novel chemogenetic activation strategy exploiting insect olfactory ionotropic receptors (IRs), termed "IR-mediated neuronal activation," and used it for selective stimulation of NE neurons in the locus coeruleus (LC). Drosophila melanogaster IR84a and IR8a subunits were expressed in LC NE neurons in transgenic mice. Application of phenylacetic acid (a specific ligand for the IR84a/IR8a complex) at appropriate doses induced excitatory responses of NE neurons expressing the receptors in both slice preparations and in vivo electrophysiological conditions, resulting in a marked increase of NE release in the LC nerve terminal regions (male and female). Ligand-induced activation of LC NE neurons enhanced the retrieval process of conditioned taste aversion without affecting taste sensitivity, general arousal state, and locomotor activity. This enhancing effect on taste memory retrieval was mediated, in part, through α1- and β-adrenergic receptors in the basolateral nucleus of the amygdala (BLA; male). Pharmacological inhibition of LC NE neurons confirmed the facilitative role of these neurons in memory retrieval via adrenergic receptors in the BLA (male). Our findings indicate that the LC NE system, through projections to the BLA, controls the retrieval process of taste associative memory.SIGNIFICANCE STATEMENT Norepinephrine (NE)-containing neurons in the brain play a key role in the modulation of synaptic plasticity underlying various processes of memory formation, but the role of the NE system in memory retrieval remains unclear. We developed a chemogenetic activation system based on insect olfactory ionotropic receptors and used it for selective stimulation of NE neurons in the locus coeruleus (LC) in transgenic mice. Ligand-induced activation of LC NE neurons enhanced the retrieval of conditioned taste aversion, which was mediated, in part, through adrenoceptors in the basolateral amygdala. Pharmacological blockade of LC activity confirmed the facilitative role of these neurons in memory retrieval. Our findings indicate that the LC-amygdala pathway plays an important role in the recall of taste associative memory.

    DOI： 10.1523/JNEUROSCI.1720-20.2020

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  • Neuroligin3 splice isoforms shape inhibitory synaptic function in the mouse hippocampus. Reviewed International journal

    Motokazu Uchigashima, Ming Leung, Takuya Watanabe, Amy Cheung, Timmy Le, Sabine Pallat, Alexandre Luis Marques Dinis, Masahiko Watanabe, Yuka Imamura Kawasawa, Kensuke Futai

    The Journal of biological chemistry   295 ( 25 )   8589 - 8595   2020.6

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    Synapse formation is a dynamic process essential for the development and maturation of the neuronal circuitry in the brain. At the synaptic cleft, trans-synaptic protein-protein interactions are major biological determinants of proper synapse efficacy. The balance of excitatory and inhibitory synaptic transmission (E-I balance) stabilizes synaptic activity, and dysregulation of the E-I balance has been implicated in neurodevelopmental disorders, including autism spectrum disorders. However, the molecular mechanisms underlying the E-I balance remain to be elucidated. Here, using single-cell transcriptomics, immunohistochemistry, and electrophysiology approaches to murine CA1 pyramidal neurons obtained from organotypic hippocampal slice cultures, we investigate neuroligin (Nlgn) genes that encode a family of postsynaptic adhesion molecules known to shape excitatory and inhibitory synaptic function. We demonstrate that the NLGN3 protein differentially regulates inhibitory synaptic transmission in a splice isoform-dependent manner at hippocampal CA1 synapses. We also found that distinct subcellular localizations of the NLGN3 isoforms contribute to the functional differences observed among these isoforms. Finally, results from single-cell RNA-Seq analyses revealed that Nlgn1 and Nlgn3 are the major murine Nlgn genes and that the expression levels of the Nlgn splice isoforms are highly diverse in CA1 pyramidal neurons. Our results delineate isoform-specific effects of Nlgn genes on the E-I balance in the murine hippocampus.

    DOI： 10.1074/jbc.AC120.012571

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  • Methodological approaches to understand the molecular mechanism of structural plasticity of dendritic spines. International journal

    Takayasu Mikuni, Motokazu Uchigashima

    The European journal of neuroscience   54 ( 8 )   6902 - 6911   2020.4

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    Dendritic spines are tiny protrusions emanating from the neuronal dendrites, typically housing single excitatory postsynapses. Structural plasticity of dendritic spines is considered to be essential for synaptic functional plasticity and also reorganization of neural circuits during learning and memory. Structural plasticity of spines is mediated by complex biochemical signaling with various spatial and temporal scales. A variety of methods based on pharmacological, genetic, molecular, imaging and optical approaches has been developed and applied to dissect the complex signal transduction pathways. In this review, we overview both conventional and new methodological approaches to identify, monitor and manipulate key molecules for structural plasticity of dendritic spines, ultimately aiming to understand the molecular mechanism of learning and memory in behaving animals.

    DOI： 10.1111/ejn.14734

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  • Endocannabinoid and nitric oxide systems of the hypothalamic paraventricular nucleus mediate effects of NPY on energy expenditure. Reviewed International journal

    Zoltán Péterfi, Imre Farkas, Raphael G P Denis, Erzsébet Farkas, Motokazu Uchigashima, Tamás Füzesi, Masahiko Watanabe, Ronald M Lechan, Zsolt Liposits, Serge Luquet, Csaba Fekete

    Molecular metabolism   18   120 - 133   2018.12

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    OBJECTIVE: Neuropeptide Y (NPY) is one of the most potent orexigenic peptides. The hypothalamic paraventricular nucleus (PVN) is a major locus where NPY exerts its effects on energy homeostasis. We investigated how NPY exerts its effect within the PVN. METHODS: Patch clamp electrophysiology and Ca2+ imaging were used to understand the involvement of Ca2+ signaling and retrograde transmitter systems in the mediation of NPY induced effects in the PVN. Immuno-electron microscopy were performed to elucidate the subcellular localization of the elements of nitric oxide (NO) system in the parvocellular PVN. In vivo metabolic profiling was performed to understand the role of the endocannabinoid and NO systems of the PVN in the mediation of NPY induced changes of energy homeostasis. RESULTS: We demonstrated that NPY inhibits synaptic inputs of parvocellular neurons in the PVN by activating endocannabinoid and NO retrograde transmitter systems via mobilization of Ca2+ from the endoplasmic reticulum, suggesting that NPY gates the synaptic inputs of parvocellular neurons in the PVN to prevent the influence of non-feeding-related inputs. While intraPVN administered NPY regulates food intake and locomotor activity via NO signaling, the endocannabinoid system of the PVN selectively mediates NPY-induced decrease in energy expenditure. CONCLUSION: Thus, within the PVN, NPY stimulates the release of endocannabinoids and NO via Ca2+-influx from the endoplasmic reticulum. Both transmitter systems appear to have unique roles in the mediation of the NPY-induced regulation of energy homeostasis, suggesting that NPY regulates food intake, energy expenditure, and locomotor activity through different neuronal networks of this nucleus.

    DOI： 10.1016/j.molmet.2018.08.007

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  • Monitoring and Updating of Action Selection for Goal-Directed Behavior through the Striatal Direct and Indirect Pathways. Reviewed International journal

    Satoshi Nonomura, Kayo Nishizawa, Yutaka Sakai, Yasuo Kawaguchi, Shigeki Kato, Motokazu Uchigashima, Masahiko Watanabe, Ko Yamanaka, Kazuki Enomoto, Satomi Chiken, Hiromi Sano, Shogo Soma, Junichi Yoshida, Kazuyuki Samejima, Masaaki Ogawa, Kazuto Kobayashi, Atsushi Nambu, Yoshikazu Isomura, Minoru Kimura

    Neuron   99 ( 6 )   1302 - 1314   2018.9

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    The basal ganglia play key roles in adaptive behaviors guided by reward and punishment. However, despite accumulating knowledge, few studies have tested how heterogeneous signals in the basal ganglia are organized and coordinated for goal-directed behavior. In this study, we investigated neuronal signals of the direct and indirect pathways of the basal ganglia as rats performed a lever push/pull task for a probabilistic reward. In the dorsomedial striatum, we found that optogenetically and electrophysiologically identified direct pathway neurons encoded reward outcomes, whereas indirect pathway neurons encoded no-reward outcome and next-action selection. Outcome coding occurred in association with the chosen action. In support of pathway-specific neuronal coding, light activation induced a bias on repeat selection of the same action in the direct pathway, but on switch selection in the indirect pathway. Our data reveal the mechanisms underlying monitoring and updating of action selection for goal-directed behavior through basal ganglia circuits.

    DOI： 10.1016/j.neuron.2018.08.002

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  • Activity-Induced Regulation of Synaptic Strength through the Chromatin Reader L3mbtl1 Reviewed

    Wenjie Mao, Anna C. Salzberg, Motokazu Uchigashima, Yuto Hasegawa, Hanno Hock, Masahiko Watanabe, Schahram Akbarian, Yuka Imamura Kawasawa, Kensuke Futai

    Cell Reports   23 ( 11 )   3209 - 3222   2018.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier B.V.  

    DOI： 10.1016/j.celrep.2018.05.028

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  • Endocytosis following dopamine D-2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFR beta signaling in striatopallidal medium spiny neurons Reviewed

    N. Shioda, Y. Yabuki, Y. Wang, M. Uchigashima, T. Hikida, T. Sasaoka, H. Mori, M. Watanabe, M. Sasahara, K. Fukunaga

    MOLECULAR PSYCHIATRY   22 ( 8 )   1205 - 1222   2017.8

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    DOI： 10.1038/mp.2016.200

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  • LUsing a novel PV-Cre rat model to characterize pallidonigral cells and their terminations Reviewed

    Yoon-Mi Oh, Fuyuki Karube, Susumu Takahashi, Kenta Kobayashi, Masahiko Takada, Motokazu Uchigashima, Masahiko Watanabe, Kayo Nishizawa, Kazuto Kobayashi, Fumino Fujiyama

    BRAIN STRUCTURE & FUNCTION   222 ( 5 )   2359 - 2378   2017.7

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    DOI： 10.1007/s00429-016-1346-2

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  • Ventrolateral Striatal Medium Spiny Neurons Positively Regulate Food-Incentive, Goal-Directed Behavior Independently of D1 and D2 Selectivity Reviewed

    Akiyo Natsubori, Iku Tsutsui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban de Kerchove d'Exaerde, Masaru Mimura, Norio Takata, Kenji F. Tanaka

    JOURNAL OF NEUROSCIENCE   37 ( 10 )   2723 - 2733   2017.3

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    DOI： 10.1523/JNEUROSCI.3377-16.2017

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  • Dysfunction of ventrolateral striatal dopamine receptor type 2-expressing medium spiny neurons impairs instrumental motivation Reviewed

    Iku Tsutsui-Kimura, Hiroyuki Takiue, Keitaro Yoshida, Ming Xu, Ryutaro Yano, Hiroyuki Ohta, Hiroshi Nishida, Youcef Bouchekioua, Hideyuki Okano, Motokazu Uchigashima, Masahiko Watanabe, Norio Takata, Michael R. Drew, Hiromi Sano, Masaru Mimura, Kenji F. Tanaka

    Nature Communications   8   14304   2017.2

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    DOI： 10.1038/ncomms14304

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  • The Metabotropic Glutamate Receptor Subtype 1 Mediates Experience-Dependent Maintenance of Mature Synaptic Connectivity in the Visual Thalamus Reviewed

    Madoka Narushima, Motokazu Uchigashima, Yuki Yagasaki, Takeshi Harada, Yasuyuki Nagumo, Naofumi Uesaka, Kouichi Hashimoto, Atsu Aiba, Masahiko Watanabe, Mariko Miyata, Masanobu Kano

    NEURON   91 ( 5 )   1097 - 1109   2016.9

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    DOI： 10.1016/j.neuron.2016.07.035

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  • Crucial Roles of the Endocannabinoid 2-Arachidonoylglycerol in the Suppression of Epileptic Seizures Reviewed

    Yuki Sugaya, Maya Yamazaki, Motokazu Uchigashima, Kenta Kobayashi, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    CELL REPORTS   16 ( 5 )   1405 - 1415   2016.8

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    DOI： 10.1016/j.celrep.2016.06.083

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  • Transsynaptic Modulation of Kainate Receptor Functions by C1q-like Proteins Reviewed

    Keiko Matsuda, Timotheus Budisantoso, Nikolaos Mitakidis, Yuki Sugaya, Eriko Miura, Wataru Kakegawa, Miwako Yamasaki, Kohtarou Konno, Motokazu Uchigashima, Manabu Abe, Izumi Watanabe, Masanobu Kano, Masahiko Watanabe, Kenji Sakimura, A. Radu Aricescu, Michisuke Yuzaki

    NEURON   90 ( 4 )   752 - 767   2016.5

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    DOI： 10.1016/j.neuron.2016.04.001

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  • Territories of heterologous inputs onto Purkinje cell dendrites are segregated by mGluR1-dependent parallel fiber synapse elimination Reviewed

    Ryoichi Ichikawa, Kouichi Hashimoto, Taisuke Miyazaki, Motokazu Uchigashima, Miwako Yamasaki, Atsu Aiba, Masanobu Kano, Masahiko Watanabe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 8 )   2282 - 2287   2016.2

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    DOI： 10.1073/pnas.1511513113

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  • Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo Reviewed

    Taku Nagai, Shinichi Nakamuta, Keisuke Kuroda, Sakura Nakauchi, Tomoki Nishioka, Tetsuya Takano, Xinjian Zhang, Daisuke Tsuboi, Yasuhiro Funahashi, Takashi Nakano, Junichiro Yoshimoto, Kenta Kobayashi, Motokazu Uchigashima, Masahiko Watanabe, Masami Miura, Akinori Nishi, Kazuto Kobayashi, Kiyofumi Yamada, Mutsuki Amano, Kozo Kaibuchi

    NEURON   89 ( 3 )   550 - 565   2016.2

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  • Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay Reviewed

    Hiroyuki Takeda, Tomio Ogasawara, Tatsuhiko Ozawa, Atsushi Muraguchi, Pei-Ju Jih, Ryo Morishita, Motokazu Uchigashima, Masahiko Watanabe, Toyoshi Fujimoto, Takahiro Iwasaki, Yaeta Endo, Tatsuya Sawasaki

    SCIENTIFIC REPORTS   5   11333   2015.6

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  • VGluT3-Expressing CCK-Positive Basket Cells Construct Invaginating Synapses Enriched with Endocannabinoid Signaling Proteins in Particular Cortical and Cortex-Like Amygdaloid Regions of Mouse Brains Reviewed

    Yuki Omiya, Motokazu Uchigashima, Kohtarou Konno, Miwako Yamasaki, Taisuke Miyazaki, Takayuki Yoshida, Ichiro Kusumi, Masahiko Watanabe

    JOURNAL OF NEUROSCIENCE   35 ( 10 )   4215 - 4228   2015.3

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    DOI： 10.1523/JNEUROSCI.4681-14.2015

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  • Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice Reviewed

    Kohtarou Konno, Hiromi Takahashi-Iwanaga, Motokazu Uchigashima, Kyoko Miyasaka, Akihiro Funakoshi, Masahiko Watanabe, Toshihiko Iwanaga

    HISTOCHEMISTRY AND CELL BIOLOGY   143 ( 3 )   301 - 312   2015.3

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  • Retrograde Signaling for Climbing Fiber Synapse Elimination Reviewed

    Naofumi Uesaka, Motokazu Uchigashima, Takayasu Mikuni, Hirokazu Hirai, Masahiko Watanabe, Masanobu Kano

    CEREBELLUM   14 ( 1 )   4 - 7   2015.2

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  • Neuron type- and input pathway-dependent expression of Slc4a10 in adult mouse brains Reviewed

    Xiaohong Song, Miwako Yamasaki, Taisuke Miyazaki, Kohtarou Konno, Motokazu Uchigashima, Masahiko Watanabe

    EUROPEAN JOURNAL OF NEUROSCIENCE   40 ( 5 )   2797 - 2810   2014.9

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  • GABAergic neurons in the ventral tegmental area receive dual GABA/enkephalin-mediated inhibitory inputs from the bed nucleus of the stria terminalis Reviewed

    Takehiro Kudo, Kohtarou Konno, Motokazu Uchigashima, Yuchio Yanagawa, Ichiro Sora, Masabumi Minami, Masahiko Watanabe

    EUROPEAN JOURNAL OF NEUROSCIENCE   39 ( 11 )   1796 - 1809   2014.6

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  • Retrograde semaphorin signaling regulates synapse elimination in the developing mouse brain Reviewed

    Naofumi Uesaka, Motokazu Uchigashima, Takayasu Mikuni, Takanobu Nakazawa, Harumi Nakao, Hirokazu Hirai, Atsu Aiba, Masahiko Watanabe, Masanobu Kano

    SCIENCE   344 ( 6187 )   1020 - 1023   2014.5

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  • Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum Reviewed

    Kohtarou Konno, Keiko Matsuda, Chihiro Nakamoto, Motokazu Uchigashima, Taisuke Miyazaki, Miwako Yamasaki, Kenji Sakimura, Michisuke Yuzaki, Masahiko Watanabe

    JOURNAL OF NEUROSCIENCE   34 ( 22 )   7412 - 7424   2014.5

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    DOI： 10.1523/JNEUROSCI.0628-14.2014

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  • Chronic Alterations in Monoaminergic Cells in the Locus Coeruleus in Orexin Neuron-Ablated Narcoleptic Mice Reviewed

    Natsuko Tsujino, Tomomi Tsunematsu, Motokazu Uchigashima, Kohtarou Konno, Akihiro Yamanaka, Kazuto Kobayashi, Masahiko Watanabe, Yoshimasa Koyama, Takeshi Sakurai

    PLOS ONE   8 ( 7 )   e70012   2013.7

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  • Type 2K+Cl cotransporter is preferentially recruited to climbing fiber synapses during development and the stellate cell-targeting dendritic zone at adulthood in cerebellar Purkinje cells Reviewed

    Issei Kawakita, Motokazu Uchigashima, Kohtarou Konno, Taisuke Miyazaki, Miwako Yamasaki, Masahiko Watanabe

    EUROPEAN JOURNAL OF NEUROSCIENCE   37 ( 4 )   532 - 543   2013.2

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  • Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice Reviewed

    Takehiro Kudo, Motokazu Uchigashima, Taisuke Miyazaki, Kohtarou Konno, Miwako Yamasaki, Yuchio Yanagawa, Masabumi Minami, Masahiko Watanabe

    JOURNAL OF NEUROSCIENCE   32 ( 50 )   18035 - 18046   2012.12

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    DOI： 10.1523/JNEUROSCI.4057-12.2012

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  • Striatal Indirect Pathway Contributes to Selection Accuracy of Learned Motor Actions Reviewed

    Kayo Nishizawa, Ryoji Fukabori, Kana Okada, Nobuyuki Kai, Motokazu Uchigashima, Masahiko Watanabe, Akira Shiota, Masatsugu Ueda, Yuji Tsutsui, Kazuto Kobayashi

    JOURNAL OF NEUROSCIENCE   32 ( 39 )   13421 - 13432   2012.9

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    DOI： 10.1523/JNEUROSCI.1969-12.2012

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  • Localization of Acetylcholine-Related Molecules in the Retina: Implication of the Communication from Photoreceptor to Retinal Pigment Epithelium Reviewed

    Hidetaka Matsumoto, Koji Shibasaki, Motokazu Uchigashima, Amane Koizumi, Masashi Kurachi, Yasuhiro Moriwaki, Hidemi Misawa, Koichiro Kawashima, Masahiko Watanabe, Shoji Kishi, Yasuki Ishizaki

    PLOS ONE   7 ( 8 )   e42841   2012.8

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  • Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression Reviewed

    Asami Tanimura, Motokazu Uchigashima, Maya Yamazaki, Naofumi Uesaka, Takayasu Mikuni, Manabu Abe, Kouichi Hashimoto, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   109 ( 30 )   12195 - 12200   2012.7

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  • Lack of Molecular-Anatomical Evidence for GABAergic Influence on Axon Initial Segment of Cerebellar Purkinje Cells by the Pinceau Formation Reviewed

    Atsushi Iwakura, Motokazu Uchigashima, Taisuke Miyazaki, Miwako Yamasaki, Masahiko Watanabe

    JOURNAL OF NEUROSCIENCE   32 ( 27 )   9438 - 9448   2012.7

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    DOI： 10.1523/JNEUROSCI.1651-12.2012

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  • TRIM67 Protein Negatively Regulates Ras Activity through Degradation of 80K-H and Induces Neuritogenesis Reviewed

    Hiroaki Yaguchi, Fumihiko Okumura, Hidehisa Takahashi, Takahiro Kano, Hiroyuki Kameda, Motokazu Uchigashima, Shinya Tanaka, Masahiko Watanabe, Hidenao Sasaki, Shigetsugu Hatakeyama

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 15 )   12050 - 12059   2012.4

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  • Striatal direct pathway modulates response time in execution of visual discrimination Reviewed

    Ryoji Fukabori, Kana Okada, Kayo Nishizawa, Nobuyuki Kai, Kenta Kobayashi, Motokazu Uchigashima, Masahiko Watanabe, Yuji Tsutsui, Kazuto Kobayashi

    EUROPEAN JOURNAL OF NEUROSCIENCE   35 ( 5 )   784 - 797   2012.3

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  • Unbalance of CB1 receptors expressed in GABAergic and glutamatergic neurons in a transgenic mouse model of Huntington's disease Reviewed

    Valentina Chiodi, Motokazu Uchigashima, Sarah Beggiato, Antonella Ferrante, Monica Armida, Alberto Martire, Rosa Luisa Potenza, Luca Ferraro, Sergio Tanganelli, Masahiko Watanabe, Maria Rosaria Domenici, Patrizia Popoli

    NEUROBIOLOGY OF DISEASE   45 ( 3 )   983 - 991   2012.3

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    DOI： 10.1016/j.nbd.2011.12.017

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  • Selective Neural Pathway Targeting Reveals Key Roles of Thalamostriatal Projection in the Control of Visual Discrimination Reviewed

    Shigeki Kato, Masahito Kuramochi, Kenta Kobayashi, Ryoji Fukabori, Kana Okada, Motokazu Uchigashima, Masahiko Watanabe, Yuji Tsutsui, Kazuto Kobayashi

    JOURNAL OF NEUROSCIENCE   31 ( 47 )   17169 - 17179   2011.11

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    DOI： 10.1523/JNEUROSCI.4005-11.2011

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  • Molecular and morphological configuration for 2-arachidonoylglycerol-mediated retrograde signaling at mossy cell-granule cell synapses in the dentate gyrus. Reviewed

    Uchigashima M, Yamazaki M, Yamasaki M, Tanimura A, Sakimura K, Kano M, Watanabe M

    The Journal of neuroscience : the official journal of the Society for Neuroscience   31 ( 21 )   7700 - 7714   2011.5

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  • Unique inhibitory synapse with particularly rich endocannabinoid signaling machinery on pyramidal neurons in basal amygdaloid nucleus Reviewed

    Takayuki Yoshida, Motokazu Uchigashima, Miwako Yamasaki, Istvan Katona, Maya Yamazaki, Kenji Sakimura, Masanobu Kano, Mitsuhiro Yoshioka, Masahiko Watanabe

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   108 ( 7 )   3059 - 3064   2011.2

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    DOI： 10.1073/pnas.1012875108

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  • Rho/Rho-kinase signaling pathway controls axon patterning of a specified subset of cranial motor neurons Reviewed

    Kenta Kobayashi, Tomoyuki Masuda, Masanori Takahashi, Jun-ichi Miyazaki, Masahiro Nakagawa, Motokazu Uchigashima, Masahiko Watanabe, Hiroyuki Yaginuma, Noriko Osumi, Kozo Kaibuchi, Kazuto Kobayashi

    EUROPEAN JOURNAL OF NEUROSCIENCE   33 ( 4 )   612 - 621   2011.2

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    DOI： 10.1111/j.1460-9568.2010.07554.x

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  • Cellular expression and subcellular localization of secretogranin II in the mouse hippocampus and cerebellum Reviewed

    Taisuke Miyazaki, Miwako Yamasaki, Motokazu Uchigashima, Ayano Matsushima, Masahiko Watanabe

    EUROPEAN JOURNAL OF NEUROSCIENCE   33 ( 1 )   82 - 94   2011.1

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    DOI： 10.1111/j.1460-9568.2010.07472.x

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  • Neurochemical characterization of neurons in the bed nucleus of the stria terminalis projecting to the ventral tegmental area Reviewed

    Kudo Takehiro, Uchigashima Motokazu, Miyazaki Taisuke, Yamasaki Miwako, Minami Masabumi, Watanabe Masahiko

    NEUROSCIENCE RESEARCH   71   E322   2011

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  • Invaginating inhibitory synapse with particularly rich endocannabinoid signaling machinery in the basal nucleus of the amygdala Reviewed

    Yoshida Takayuki, Uchigashima Motokazu, Yamasaki Miwako, Katona Istvan, Yamazaki Maya, Sakimura Kenji, Kano Masanobu, Yoshioka Mitsuhiro, Watanabe Masahiko

    NEUROSCIENCE RESEARCH   71   E93 - E94   2011

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  • Cytochemical and cytological properties of perineuronal oligodendrocytes in the mouse cortex Reviewed

    Chihiro Takasaki, Miwako Yamasaki, Motokazu Uchigashima, Kohtarou Konno, Yuchio Yanagawa, Masahiko Watanabe

    European Journal of Neuroscience   32 ( 8 )   1326 - 1336   2010.10

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    DOI： 10.1111/j.1460-9568.2010.07377.x

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  • The Endocannabinoid 2-Arachidonoylglycerol Produced by Diacylglycerol Lipase alpha Mediates Retrograde Suppression of Synaptic Transmission Reviewed

    Asami Tanimura, Maya Yamazaki, Yuki Hashimotodani, Motokazu Uchigashima, Shinya Kawata, Manabu Abe, Yoshihiro Kita, Kouichi Hashimoto, Takao Shimizu, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    NEURON   65 ( 3 )   320 - 327   2010.2

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    DOI： 10.1016/j.neuron.2010.01.021

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  • Endocannabinoid-Mediated Control of Synaptic Transmission Reviewed

    Masanobu Kano, Takako Ohno-Shosaku, Yuki Hashimotodani, Motokazu Uchigashima, Masahiko Watanabe

    PHYSIOLOGICAL REVIEWS   89 ( 1 )   309 - 380   2009.1

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  • Key Modulatory Role of Presynaptic Adenosine A(2A) Receptors in Cortical Neurotransmission to the Striatal Direct Pathway Reviewed

    Cesar Quiroz, Rafael Lujan, Motokazu Uchigashima, Ana Patricia Simoes, Talia N. Lerner, Janusz Borycz, Anil Kachroo, Paula M. Canas, Marco Orru, Michael A. Schwarzschild, Diane L. Rosin, Anatol C. Kreitzer, Rodrigo A. Cunha, Masahiko Watanabe, Sergi Ferre

    THESCIENTIFICWORLDJOURNAL   9   1321 - 1344   2009

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    DOI： 10.1100/tsw.2009.143

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  • Predominant expression of phospholipase C beta 1 in telencephalic principal neurons and cerebellar interneurons, and its close association with related signaling molecules in somatodendritic neuronal elements Reviewed

    Masahiro Fukaya, Motokazu Uchigashima, Sachi Nomura, Yuta Hasegawa, Hisaya Kikuchi, Masahiko Watanabe

    EUROPEAN JOURNAL OF NEUROSCIENCE   28 ( 9 )   1744 - 1759   2008.11

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    DOI： 10.1111/j.1460-9568.2008.06495.x

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  • Evidence against GABA release from glutamatergic mossy fiber terminals in the developing hippocampus Reviewed

    Motokazu Uchigashima, Masahiro Fukaya, Masahiko Watanabe, Haruyuki Kamiya

    JOURNAL OF NEUROSCIENCE   27 ( 30 )   8088 - 8100   2007.7

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    DOI： 10.1523/JNEUROSCI.0702-07.2007

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  • Subcellular arrangement of molecules for 2-arachidonoyl-glycerol-mediated retrograde signaling and its physiological contribution to synaptic modulation in the striatum Reviewed

    Motokazu Uchigashima, Madoka Narushima, Masahiro Fukaya, Istvan Katona, Masanobu Kano, Masahiko Watanabe

    JOURNAL OF NEUROSCIENCE   27 ( 14 )   3663 - 3676   2007.4

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    DOI： 10.1523/JNEUROSCI.0448-07.2007

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  • Tonic enhancement of endocannabinoid-mediated retrograde suppression of inhibition by cholinergic interneuron activity in the striatum Reviewed

    Madoka Narushima, Motokazu Uchigashima, Masahiro Fukaya, Minoru Matsui, Toshiya Manabe, Kouichi Hashimoto, Masahiko Watanabe, Masanobu Kano

    JOURNAL OF NEUROSCIENCE   27 ( 3 )   496 - 506   2007.1

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    DOI： 10.1523/JNEUROSCI.4644-06.2007

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  • Subcellular arrangement of molecules for 2-arachidonoyl-glycerol-mediated retrograde signaling in the striatum Reviewed

    Uchigashima Motokazu, Narushima Madoka, Fukaya Masahiro, Katona Istven, Kano Masanobu, Watanabe Masahiko

    NEUROSCIENCE RESEARCH   58   S75   2007

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  • Depolarization-induced suppression of inhibition mediated by endocannabinoids at synapses from fast-spiking interneurons to medium spiny neurons in the striatum Reviewed

    Madoka Narushima, Motokazu Uchigashima, Kouichi Hashimoto, Masahiko Watanabe, Masanobu Kano

    EUROPEAN JOURNAL OF NEUROSCIENCE   24 ( 8 )   2246 - 2252   2006.10

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    DOI： 10.1111/j.1460-9568.2006.05119.x

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  • Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor. Reviewed

    Yoshida T, Fukaya M, Uchigashima M, Miura E, Kamiya H, Kano M, Watanabe M

    The Journal of neuroscience : the official journal of the Society for Neuroscience   26   4740 - 4751   2006.5

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  • Spine targeting of endocannabinoid synthesizing enzyme, diacylglycerol lipase-alpha in the cerebllum and hippocampus Reviewed

    Yoshida Takayuki, Fukaya Masahiro, Uchigashima Motokazu, Miura Eriko, Kamiya Haruyuki, Kano Masanobu, Watanabe Masahiko

    NEUROSCIENCE RESEARCH   55   S77   2006

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  • Development of High-throuput Methods to Visualize Synaptic Proteins in the Tissue

    内ヶ島基政

    新潟医学会雑誌   136 ( 3 )   2022

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  • 青斑核ノルアドレナリン神経細胞の活動亢進による情動記憶の想起の促進 新規神経活動活性化ツールによる検討

    井口 善生, 深堀 良二, 加藤 成樹, 高橋 和巳, 永福 智志, 辻 真伍, 挾間 章博, 内ヶ島 基政, 渡辺 雅彦, 水間 広, 崔 翼龍, 尾上 浩隆, 疋島 啓吾, 八十島 安伸, 小山内 実, 稲垣 良, 福永 浩司, 西條 琢真, 籾山 俊彦, リチャード・ベントン, 小林 和人

    日本生理学雑誌   83 ( 2 )   39 - 39   2021.5

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  • Enhanced emotional memory retrieval by chemogenetic activation of locus coeruleus norepinephrine neurons

    Fukabori, R, Iguchi, Y, Kato, S, Takahashi, K, Eifuku, S, Tsuji, S, Hazama, A, Uchigashima, M, Watanabe, M, Mizuma, H, Cui, Y, Onoe, H, Hikishima, K, Yasoshima, Y, Osanai, M, Inagaki, R, Fukunaga, K, Nishijo, T, Momiyama, T, Benton, R, Kobayashi, K

    Mendeley Data   2019.11

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  • Requirement of mGluR1 for Experience-Dependent Remodeling of Mouse Retinogeniculate Synapses

    M. Kano, M. Narushima, M. Uchigashima, M. Watanabe, A. Aiba, M. Miyata

    CURRENT NEUROPHARMACOLOGY   12   30 - 31   2014

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  • Lack of Molecular-Anatomical Evidence for GABAergic Influence on Axon Initial Segment of Cerebellar Purkinje Cells by the Pinceau Formation

    87 ( 6 )   273 - 273   2012.11

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  • Unique inhibitory synapse with particularly rich endocannabinoid signaling machinery on pyramidal neurons in basal amygdaloid nucleus

    87 ( 2 )   72 - 72   2012.4

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  • Unique inhibitory Synapse with Particularly Rich Endocannabinoid Signaling Machinery on Pyramidal Neurons in Basal Amygdaloid Nucleus

    T. Yoshida, M. Uchigashima, M. Yamasaki, I. Katona, M. Yamazaki, K. Sakimura, M. Kano, M. Yoshioka, M. Watanabe

    CURRENT NEUROPHARMACOLOGY   9   68 - 68   2011.9

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  • Behavioral role of thalamostriatal neural pathway in conditional discrimination paradigm

    Shigeki Kato, Masahito Kuramochi, Kenta Kobayashi, Ryoji Fukabori, Motokazu Uchigashima, Masahiko Watanabe, Yuji Tsutsui, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH   71   E280 - E280   2011

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    DOI： 10.1016/j.neures.2011.07.1222

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  • Striatal direct pathway modulates response time in execution of conditional discrimination

    Ryoji Fukabori, Kana Okada, Kayo Nishizawa, Nobuyuki Kai, Kenta Kobayashi, Motokazu Uchigashima, Masahiko Watanabe, Yuji Tsutsui, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH   71   E379 - E380   2011

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    DOI： 10.1016/j.neures.2011.07.1667

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  • Immunohistochemical characterization of dopaminergic synapses in the mouse striatum

    Motokazu Uchigashima, Masahiko Watanabe

    NEUROSCIENCE RESEARCH   71   E111 - E111   2011

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    DOI： 10.1016/j.neures.2011.07.470

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  • Synapse "non-specific" degradation of the endocannabinoid 2-arachidonoylglycerol mediating depolarization-induced retrograde synaptic suppression in cerebellar Purkinje cells

    Asami Tanimura, Maya Yamazaki, Motokazu Uchigashima, Naofumi Uesaka, Takayasu Mikuni, Kouichi Hashimoto, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    NEUROSCIENCE RESEARCH   71   E56 - E56   2011

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    DOI： 10.1016/j.neures.2011.07.235

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  • Molecular-anatomical basis for the spread of endocannabinoid 2-arachidonoylglycerol at mossy cell-granule cell synapses in the dentate gyrus

    Motokazu Uchigashima, Masahiko Watanabe

    NEUROSCIENCE RESEARCH   68   E55 - E55   2010

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    DOI： 10.1016/j.neures.2010.07.012

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  • Molecular mechanism of endocannabinoid-mediated retrograde signaling in basolateral amygdala

    Takayuki Yoshida, Motokazu Uchigashima, Hiroki Shikanai, Takashi Izumi, Taku Yamaguchi, Masahiko Watanabe, Mitsuhiro Yoshioka

    JOURNAL OF PHARMACOLOGICAL SCIENCES   112   62P - 62P   2010

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  • 2-Arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission in the CNS

    Asami Tanimura, Maya Yamazaki, Yuki Hashimotodani, Motokazu Uchigashima, Shinya Kawata, Manabu Abe, Yoshihiro Kita, Kouichi Hashimoto, Takao Shimizu, Masahiko Watanabe, Kenji Sakimura, Masanobu Kano

    NEUROSCIENCE RESEARCH   68   E88 - E88   2010

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    DOI： 10.1016/j.neures.2010.07.153

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  • Role of the striatonigral neurons in basal ganglia in the performance of conditional discrimination task

    Ryoji Fukabori, Kana Okada, Nobuyuki Kai, Kenta Kobayashi, Yuji Tsutsui, Motokazu Uchigashima, Masahiko Watanebe, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH   68   E185 - E185   2010

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    DOI： 10.1016/j.neures.2010.07.2391

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  • The role of dopamine D-2 receptor-expressing neurons in the nucleus accumbens core in operant matching

    Nobuyuki Kai, Ryoji Fukabori, Yuji Tsutsui, Motokazu Uchigashima, Masahiko Watanebe, Akira Shiota, Masatsugu Ueda, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH   68   E289 - E290   2010

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    DOI： 10.1016/j.neures.2010.07.1286

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  • Endocannabinoid and serotonin modulate GABAergic and glutamatergic transmission in the basolateral amygdala

    Takayuki Yoshida, Motokazu Uchigashima, Takeshi Izumi, Taku Yamaguchi, Masanobu Kano, Masahiko Watanabe, Mitsuhiro Yoshioka

    NEUROSCIENCE RESEARCH   68   E229 - E229   2010

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    DOI： 10.1016/j.neures.2010.07.1012

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  • Role of the striatopallidal neurons in basal ganglia in the performance of conditional discrimination task

    Kayo Nishizawa, Ryoji Fukabori, Nobuyuki Kai, Yuji Tsutsui, Motokazu Uchigashima, Masahiko Watanebe, Akira Shiota, Masatsugu Ueda, Kazuto Kobayashi

    NEUROSCIENCE RESEARCH   68   E185 - E185   2010

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    DOI： 10.1016/j.neures.2010.07.2390

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  • IMMUNOHISTOCHEMICAL LOCALIZATION OF MOLECULES FOR 2-ARACHIDONOYLGLYCEROL-MEDIATED RETROGRADE SIGNALING IN THE MOUSE DENTATE GYRUS

    Motokazu Uchigashima, Masahiko Watanabe

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   146 - 146   2009

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  • Retrograde signalling is induced by endocannabinoids in the basolateral amygdala

    Takayuki Yoshida, Motokazu Uchigashima, Hiroki Shikanai, Takashi Izumi, Taku Yamaguchi, Masahiko Watanabe, Mitsuhiro Yoshioka

    JOURNAL OF PHARMACOLOGICAL SCIENCES   109   220P - 220P   2009

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  • Involvement of type 1 metabotoropic glutamate receptor in developmental synapse elimination in the lateral geniculate nucleus

    Madoka Narushima, Uchigashima Motokazu, Asami Tanimura, Kouichi Hashimoto, Atsu Aiba, Masahiko Watanabe, Masanobu Kano

    NEUROSCIENCE RESEARCH   61   S57 - S57   2008

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Awards

  • 新潟大学学長賞（若手教員研究奨励）

    2022.9   新潟大学  

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  • 新潟大学優秀論文表彰

    2021.12   新潟大学  

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  • 新潟大学学長賞

    2021.8   新潟大学  

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  • 優秀論文賞

    2017.2   北海道大学大学院医学研究科   Dopamine synapse is a neuroligin-2–mediated contact between dopaminergic presynaptic and GABAergic postsynaptic structures

    内ヶ島基政

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  • フラテ研究奨励賞

    2022.2   北海道大学医学部同窓会  

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  • エクセレントティーチャー優秀賞

    2020.12   北海道大学医学部  

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  • ベストプレゼンテーション賞

    2018.9   第64回日本解剖学会東北・北海道連合支部学術集会   孤束核・分界条床核投射系のノルアドレナリン/グルタミン酸共放出ニューロンがもたらすユニークな神経伝達の分子形態学的基盤

    内ヶ島基政

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  • 若手優秀発表賞

    2015.12   包括脳ネットワーク   Stratal dopamine synapses are neuroligin-2-mediated heterologous contacts between dopaminergic pre synapse and GABAergic post synapse

    内ヶ島基政

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  • 優秀教員賞

    2014   北海道大学医学部  

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  • 第15回グリアクラブ賞

    2010.2   Molecular-anatomical architecture of 2-arachidonoyl-glycerol-mediated retrograde signaling in the mossy cell-recipient dentate molecular layer

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  • 奨励賞

    2007.12   日本顕微鏡学会北海道支部   マウス線条体における内因性カンナビノイド 2-アラキドノイルグリセロールを介した逆行性伝達抑制の分子機構

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  • 音羽博次奨学基金

    2006   北海道大学大学院医学研究科  

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Research Projects

  • 内在タンパク質のターンオーバーに基づく1細胞シナプス可塑性マッピングの開発と応用

    Grant number：24K02130

    2024.4 - 2028.3

    System name：科学研究費助成事業

    Research category：基盤研究(B)

    Awarding organization：日本学術振興会

    内ヶ島 基政

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    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

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  • AMPA受容体シナプス外膜プールに基づく認知予備能の理解

    2024 - 2030

    System name：戦略的な研究開発の推進 創発的研究支援事業

    Awarding organization：科学技術振興機構

    内ヶ島 基政

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    Authorship：Principal investigator 

    脳のニューロン間の情報伝達を担うシナプスは、幼年だけでなく大人でも可塑性と呼ばれる変化能を備えます。しかし、その生涯にわたる時空間変容の詳細は不明です。本研究では、シナプス可塑性を担うAMPA受容体シナプス外膜プールの脳内時空間定量マッピングを通じて、大人におけるシナプス可塑性の時空間的多様性とそれを生み出すメカニズムを解明し、加齢に伴う認知機能低下を防ぐ認知予備能の生物学的基盤の理解を目指します。

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  • 生きた脳組織における動的シナプトミクス解析への挑戦

    Grant number：23K18160

    2023.6 - 2025.3

    System name：科学研究費助成事業

    Research category：挑戦的研究(萌芽)

    Awarding organization：日本学術振興会

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    Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）

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  • Analysis of Molecular Dynamics for Structural Spine Plasticity during the Critical Period

    Grant number：20H05918

    2020.11 - 2025.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (A)

    Research category：Grant-in-Aid for Transformative Research Areas (A)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\59150000 （ Direct Cost: \45500000 、 Indirect Cost：\13650000 ）

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  • Inducing lifelong plasticity (iPlasticity) by brain rejuvenation: elucidation and manipulation of critical period mechanisms

    Grant number：20H05914

    2020.11 - 2025.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Transformative Research Areas (A)

    Research category：Grant-in-Aid for Transformative Research Areas (A)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\235430000 （ Direct Cost: \181100000 、 Indirect Cost：\54330000 ）

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  • Development of a novel method for analyzing the distribution of endogenous proteins in mammalian brain tissues

    Grant number：20K21461

    2020.7 - 2022.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Challenging Research (Exploratory)

    Awarding organization：Japan Society for the Promotion of Science

    Uchigashima Motokazu

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    Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）

    The localization of endogenous proteins is essential for understanding cellular functions. Although immunohistochemistry is a prevalent method to examine the anatomical information of proteins of interest (POIs), it sometimes fails the detection of POIs at dense structures due to an insufficient penetration of large antibodies against POIs. To overcome this issue, we developed a novel method to quantitatively detect endogenous POIs at single cell levels via small fluorescent ligand-mediated labeling of chemical tags which were fused to POIs by in vivo genome editing techniques.

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  • Interrogation of the spatiotemporal integration of dopaminergic signaling in striatal medium spiny neurons

    Grant number：20H03349

    2020.4 - 2023.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Uchigashima Motokazu

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    Grant amount：\17940000 （ Direct Cost: \13800000 、 Indirect Cost：\4140000 ）

    Dopaminergic transmission is essential for our normal brain function, and its dysfunction causes many brain disorders such as Parkinson's disease and schizophrenia. However, our current knowledge is not sufficient about where dopamine is released from and what it targets to, and how it functions on its target cells. To address these questions, we newly developed a genome editing-based platform to visualize molecularly-defined dopamine release sites and simultaneously introduce Cre recombinase for neuronal labeling or manipulation in the same neurons in mammalian brains.

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  • ドーパミンシナプスの生細胞イメージングを介したドーパミン神経伝達の理解

    2020.1 - 2020.12

    System name：研究助成

    Awarding organization：興和生命科学振興財団

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\1000000

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  • ドーパミンシナプスの構造的可塑性に関する研究

    2018

    System name：若手研究者研究加速事業

    Awarding organization：北海道大学

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    Authorship：Principal investigator 

    Grant amount：\8200000

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  • Study of the molecular-anatomical basis common to neuromodulatory synapses

    Grant number：15K06732

    2015 - 2017

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Uchigashima Motokazu

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    We examined by immunohistochemistry the molecular composition of synaptic structures which release neuromodulators including dopamine, acetylcholine, and noradrenaline in the brain. We revealed that these synapses were composed of non-GABAergic presynaptic structures and GABAergic postsynaptic membranes. This mismatch between presynaptic and postsynaptic structures at distinct neuromodulator synapses suggests GABAergic postsynaptic molecules commonly anchor the molecular machinery to release neuromodulators to target structures.

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  • Histological study of functional molecules involved in acquirement of developmental function for eating

    Grant number：25463163

    2013.4 - 2016.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TAKASAKI Chihiro, UCHIGASHIMA Motokazu, WATANABE Masahiko

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    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    Rapid transport of monocarboxylates is essential for carbohydrate, fat, and amino acid metabolisms. The transport is facilitated by proton-linked monocarboxylate transporters (MCTs). MCT1, MCT2 and MCT4 are highly expressed in the brain. In the present study, we examined cellular expression of MCT1 in the mouse brain by fluorescent in situ hybridization and immunohistochemistry. The results showed that dissociated transcription and translational control in neurons was found in pyramidal cells in the CA1 of hippocampus and cholinergic neurons in the dorsal motor nucleus of vagus nerve. Therefore, neuronal expression of MCT1 is transcriptionally active, but suppressed at the post-transcription levels. Through this mechanism, predominant MCT1 expression in astrocytes and capillary endothelial cells is constructed in the brain.

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  • Functional morphology of dopamine synapses in the mouse striatum

    Grant number：25830031

    2013 - 2014

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Young Scientists (B)

    Awarding organization：Japan Society for the Promotion of Science

    UCHIGASHIMA Motokazu

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    Midbrain dopaminergic neurons form a number of so-called dopamine synapses on stratal neurons, and have a strong influence on motor and cognitive functions. However, the molecular composition of dopamine synapses remains elusive. Here we found that dopamine synapses were heterologous contacts between a dopaminergic presynapse and GABAergic postsynapse in the mouse striatum, and synaptic adhesion molecule neuroligin-2 was involved in their formation. These suggest that dopamine synapses can increase the potency and selectivity of dopaminergic modulation by the anchorage of dopamine release sites to target stratal neurons.

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  • Molecular-anatomical research on multi-modal regulation of synaptic transmission in higher brain regions

    Grant number：24220007

    2012.5 - 2017.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (S)

    Awarding organization：Japan Society for the Promotion of Science

    WATANABE MASAHIKO, Yamasaki Miwako, Miyazaki Taisuke, Konno Kohtarou, Uchigashima Motokazu, Kano Masanobu, Shigemoto Ryuichi, Kobayashi Kazuto

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    Grant amount：\196820000 （ Direct Cost: \151400000 、 Indirect Cost：\45420000 ）

    state-dependent manners. We found that TARP and GluD families play important roles in input-, target cell-, activity-dependent regulations in the cerebellum and hippocampus. In the cortex and cortex-like amygdala, CCK-positive interneurons expressing VGluT3 constructed unique invaginating synapses, which were also characterized by intense expression of endocannabinoid signaling molecules to powerfully drive activity- and state-dependent disinhibition. Furthermore, so-called ‘dopamine synapses’ are important for state-dependent controls of motor and cognitive functions. We address that dopamine synapses were neuroligin-2-mediated heterologous contacts formed between dopaminergic presynapse and GABAergic postsynapse, from which we propose the third mode of neural transmission, anchored transmission, in addition to classical modes of wired and volume transmissions.

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  • Analysis for endocannabinoid signaling in the epileptogenic circuit

    Grant number：23800001

    2011 - 2012

    System name：Grants-in-Aid for Scientific Research(研究活動スタート支援)

    Research category：研究活動スタート支援, 研究活動スタート支援

    Awarding organization：Ministry of Education, Culture, Sports, Science and Technology

    Motokazu UCHIGASHIMA

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\3250000 （ Direct Cost: \2500000 、 Indirect Cost：\750000 ）

    This research project reveled the molecular anatomical basis fora major endocannabinoid, 2-arachidonoyl glycerol (2-AG), to effectively inhibit synaptic transmission at mossy cell-granule cell synapses, which constitute a potentiaepilectogenic circuit in the CNS. This suggests that 2-AG plays a role as a synapticcircuit breaker to prevent the epiletogenesis.

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  • 線条体におけるドーパミンD2受容体依存的シナプス長期抑制機構の解明

    Grant number：08J04030

    2008 - 2010

    System name：科学研究費助成事業

    Research category：特別研究員奨励費

    Awarding organization：日本学術振興会

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    Grant amount：\1800000 （ Direct Cost: \1800000 ）

    線条体は随意運動の調節や認知機能に重要な神経核であり、中脳からのドーパミン作動性投射による強力な制御を受けている。その中でも、皮質線条体路シナプスで認められるドーパミンD2受容体依存的長期抑圧は、線条体の機能発現に重要と考えられているが、その分子形態学的基盤および生理機能について不明点が多い。
    本年度は、間接路中型有棘ニューロンに発現するD2受容体と、ドーパミン作動性軸索との空間的位置関係を明らかにするため、免疫組織化学的手法を用いた解析を主に行った。間接路中型ニューロンにおけるD2受容体は、ポストシナプス側となる細胞要素にほぼ選択的に分布し、とりわけ樹状突起で最も豊富な分布を示したが、スパインとシナプスを作る大脳皮質由来のグルタミン酸作動性終末ではほとんど観察されなかった。一方、ドーパミン作動性軸索は、線条体内においてシナプス構造の形成を認めた。D2受容体を発現する中型有棘ニューロンともシナプス構造を高頻度に形成していたが、そのポストシナプス側となる細胞膜上にはD2受容体が集積するような傾向は認められなかった。
    これらの分子形態基盤は、シナプス部に受容体が集積するグルタミン酸あるいはGABA作動性シナプスとは異なっており、ドーパミン伝達がシナプス非選択的なボリューム伝達の様式をもって行われていることを示している。さらに、D2受容体を介した長期抑圧現象に対しても、ドーパミンはシナプス非選択的な制御を行っていると予想される。

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