Authorship：Last author   Language：Japanese   Publisher：The Ecological Society of Japan  

Many endangered hygrophytes and hydrophytes appeared in habitats created by the tsunami disaster following the 2011 Tohoku Earthquake and subsequently disappeared following the reconstruction work. We surveyed the aquatic flora of a small wetland on Nono-shima Island, Miyagi Prefecture, Japan, in 2015 that had been disturbed by the 2011 Tohoku Earthquake and tsunami. Four brackish water submerged vascular plant species and one charalean alga were recorded. The brackish water submerged macrophyte species diversity of this wetland was higher than that of salt marshes in Japan. As shown by aerial photographs and satellite images, this wetland is behind a beach ridge and was a paddy field or fallow land for at least 50 years before the tsunami. There was insufficient time to consider conservation plans for the rare species that emerged after the disaster because of the rapid reconstruction of infrastructure required in the disaster-stricken areas. To provide information for species conservation rapidly, it may be effective to predict the emergence of species from buried soil seeds using land use history and topographical information.

DOI： 10.18960/hozen.1924

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Other Link： https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H04146/

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/ajb2.1462

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ajb2.1462

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATL SCIENCE MUSEUM & KOREAN NATL ARBORETUM  

A small semiartificial pond, revealing high species richness with respect to the aquatic macrophytes, was studied in the Aomori Prefecture, Japan. The pond had been completely formed ca. 10 years previously after excavation into a wet bog. To identify and evaluate its aquatic macrophyte flora during 2017-2018, we listed the species of aquatic vascular plants and charophytes in the pond and compared this list with those from the natural Japanese lakes using a database of aquatic flora in Japan. Two species were identified by molecular analyses because they lacked any reproductive organs, which were necessary for identifying the species level. We found a total of 57 taxa of aquatic macrophytes including 15 Red List species in Japan or Aomori Prefecture. Comparing the flora list with those from the 66 natural Japanese lakes surveyed since 2001, the pond was ranked fourth in the order of diversity (based on species richness) and rarity (based on numbers of Red List species). Therefore, the pond is an important aquatic environment in terms of aquatic macrophyte conservation in Japan. Interestingly, for a pond with such high species diversity, it is relatively small (0.14 km(2)), semiartificial, and relatively recently formed. (C) 2019 National Science Museum of Korea (NSMK) and Korea National Arboretum (KNA), Publishing Services by Elsevier.

DOI： 10.1016/j.japb.2019.02.006

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Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.24769/00000066

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Language：Japanese   Publisher：山梨大学教育学部  

山梨県の水田域において2016年に120定点で行った定性調査の結果と2017年に161定点で行った定量調査の結果を基に，各市町村間で群集類似度をHornの重複度指数により算出し，クラスター解析を最短距離法により行った。類似度は甲斐市と北杜市で最も高く，次いで甲府市と南アルプス市，昭和町と南アルプス市であり，隣接するか距離的に近い市町村間で高い傾向が見られた。これは距離的に近い場所では環境が似ていることや，水草・大型藻類の移入頻度などに起因すると考えられた。クラスター解析では東部・富士五湖地方の4市町から成るクラスターと中西部の12市町全てを含んだクラスターが形成された。これら2つの地域は山によって隔てられていることから，標高や気温などが異なり，それらが水草・大型藻類の種組成や生育量に影響したものと推察された。

DOI： 10.34429/00001081

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DOI： 10.3791/57761

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Netherlands  

Polymorphic simple sequence repeat (SSR) markers of Ecklonia radicosa were developed using Illumina MiSeq next-generation sequencing (NGS) analysis. In total, 3112 SSRs (di- to hexanucleotide motifs repeated more than six times) were identified from 61.5-Mb assembled genomic DNA using MISA perl script. Among the SSRs, di- (853 loci, 27.4%) and trinucleotides (1813 loci, 58.3%) were dominant, while tetra- (172 loci, 5.5%), penta- (175 loci, 5.6%), and hexanucleotides (99 loci, 3.2%) were not common. After specific amplification and polymorphic tests of 75 selected loci (tri- to hexanucleotide motifs repeated more than eight times), 11 SSR markers (Eradic01–11) were successfully developed. The range of the number of alleles, observed heterozygosity, and expected heterozygosity in the 11 markers was 3–13, 0.200–0.683, and 0.258–0.864, respectively. Polymorphic information content (PIC) analysis indicated that eight markers (Eradic01, 02, 04, 05, 06, 08, 09, and 11) were highly informative (PIC &gt
0.5) and the other three were reasonably informative (0.5 &lt
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0.25). In addition, intra-genus amplification was obtained in all markers except for Eradic02, 08, and 11. These markers could help to reveal the genetic diversity and population structure of E. radicosa.

DOI： 10.1007/s10811-018-1406-5

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

The taxonomic status of Nitella flexilis var. bifurcata (Charales, Characeae), which is endemic to Japan, has remained unclear because there have been no records since its description in 1964, and the detailed morphology of fully mature individuals as well as its molecular phylogeny have not been studied. Recently, we collected a fully mature N.flexilis var. bifurcata from two lakes near the type locality in Japan. The morphological characteristics of the thalli agreed well with that in the original description of the taxon, with distinctive fertile and sterile branchlets that divided twice and unicellular dactyls. Scanning electron microscopy showed that the fine oospore structure of N.flexilis var. bifurcata was different from that of related taxa. In addition, molecular phylogenetic and haplotype network analyses using the chloroplast rbcL DNA sequences and the intergenic spacer regions between atpB and rbcL genes demonstrated that N.flexilis var. bifurcata and N.flexilis var. flexilis were distinct.

DOI： 10.1111/pre.12085

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

Nitellopsis obtusa (Charales, Charophyceae) are widely distributed from Europe to Asia; however, this species has been recorded in only the five lakes in central Honshu in Japan. This species was thought to be extinct in Japan, but was rediscovered in limited areas of Lake Kawaguchi in central Honshu. More recently, we discovered more Japanese populations of N. obtusa in Lake Biwa in western Honshu, and it became clear that the species had a broader distribution in Japan than originally believed. In addition, although only male or female thalli have been collected at each lake, both male and female thalli were found from Lake Biwa. This is the first report of a potentially sexual population of N. obtusa in Japan. The DNA sequences of three chloroplast DNA markers, including both coding and non-coding regions, were identical in all specimens from Lake Kawaguchi and Lake Nojiri (Central Honshu), and differed from those of Lake Biwa and German specimens. Although Japanese and German specimens were genetically similar, Japanese specimens displayed considerable genetic diversity according to locality.

DOI： 10.1111/pre.12057

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：山梨県環境科学研究所  

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Language：Japanese   Publisher：日本藻類学会  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCI LTD  

Optimum conditions of temperature, salinity and glucose concentration were investigated for squalene production of the strain of Aurantiochytrium sp. 18W-13a, with a high content of squalene. Squalene production by this strain was optimum at 25 degrees C, 25-50% seawater concentration and 2-6% glucose concentration. When this strain was grown in the optimum condition, the squalene content and production of approximately 171 mg/g dry weight and 0.9 g/L. were much higher than that previously reported in thraustochytrids, plants and yeasts, respectively. Therefore, 18W-13a could be used as an alternative source of commercial squalene. (C) 2011 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.biortech.2011.09.127

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Language：English   Publisher：ELSEVIER SCIENCE BV  

A Botyrococcus braunii strain with high potential for biofuel production was isolated from a pond in Southeast Asia. This strain showed a higher growth rate (mu=0.101/day) and biomass productivity (2.61 dry cell weight (dcw) g/L), both of which are comparable to those of our benchmark strain B. braunii BOT-22. Chemical analyses indicated that this strain produced >44% of lipids (w/dcw), 21-37% of which was identified as C-34 botryococcene. 18S rDNA sequence analysis indicated this strain is closely related to BOT-22, suggesting that this strain can be affiliated into Race B. Interestingly, microscopic observations revealed that this strain harboured numerous bacterial ectosymbionts at the rim of host Botryococcus colonies. Our results suggest that algal-bacterial symbiotic consortia may have a potential to improve the productivity of biofuels, providing us with an alternative to pure culture of oil-producing algae. (C) 2012 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of International Organizing Committee for AOAIS-1

DOI： 10.1016/j.proenv.2012.05.005

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DOI： 10.1111/j.1529-8817.2011.01037.x

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：UNIV TOKYO CYTOLOGIA  

Chara altaica A. Braun (Charales, Charophyceae), a monoecious species of the section Desvauxia, was newly found in Japan and studied by light and scanning electron microscopy (SEM) as well as gene sequence analysis of the large subunit of Rubisco (rbcL). Our first SEM observations of C. altaica oospores revealed that small papillae were scattered on the fossa wall, an SEM oospore wall ornamentation that is essentially different from that of the monoecious species C. (sect. Desvauxia) evoluta T. F. Allen. Phylogenetic analysis demonstrated that C. altaica and 10 European samples of the dioecious species C. (sect. Desvauxia) canescens Desvaux & Loiseleur-Deslongchamps formed a robust monophyletic group, in which the rbcL gene sequence from C. altaica is identical to those from 4 parthenogenetic samples, but different from 6 other bisexual and parthenogenetic samples of C. canescens.

DOI： 10.1508/cytologia.75.211

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DOI： 10.1016/j.ympev.2009.08.015

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：INT PHYCOLOGICAL SOC  

Chara braunii is a charalean species that grows in a range of aquatic habitats from shallow water in paddy fields to the bottom of deep lakes. More than 30 infraspecific taxa have been proposed within this species because of its morphological variability. In order to reexamine the infraspecific taxa and habitat preference of C. braunii, we carried out molecular phylogenetic analyses and quantitative measurements of morphological characters used for infraspecific taxa, based on samples collected from various habitats and localities in Japan. Sequence data from the gene encoding the large subunit of Rubisco (rbcL) and the intergenic spacer regions between the beta subunit of the ATP synthase and rbcL genes demonstrated two robust clades (groups A and B). Samples of group A were collected mainly from shallow aquatic environments (< 15 cm in depth) such as paddy fields, while those of group B were mainly composed of samples found in deep aquatic environments (> 1 m in depth) such as lakes or ponds. Groups A and B therefore seem to represent closely related entities that have recently differentiated and might adapt to two different aquatic environments. However, the morphological measurements suggested these groups can be delineated by neither of the traditional diagnoses that were different between infraspecific taxa of Japanese plants of C. braunii.

DOI： 10.2216/07-27.1

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：UNIV TOKYO CYTOLOGIA  

Mature zygotes of the cosmopolitan snow alga Chloromonas nivalis (Chodat) Hoham & Mullet [=Scotiella nivalis (Chodat) Fritsch] collected from Mt. Gassan, Yamagata Prefecture, were examined by scanning electron microscopy (SEM) and single-cell sequencing of the large subunit of Rubisco (rbcL) genes. I-lie present SEM demonstrated that Japanese samples have at least two types of flanges on the zygote wall (straight and sigmate). Although these types fell within the morphological variability of C nivalis as reported previously, at least two types of rbcL (large Subunit of Rubisco) gene sequences were detected by Using the single-cell sequencing. Although the information of the rh(L genes were limited (340-400 bp), the present phylogenetic analyses revealed that these two Japanese types and C. nivalis originating front North America belong to the lineage composed of Chloromonas snow taxa. Thus, C. nivalis might involve multiple cryptic species of Chloromonas. Further ovservations of other stages of life cycle and more detailed phylogenetic analysis are needed to reveal the correct taxonomic identifications of C. nivalis.

DOI： 10.1508/cytologia.73.91

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DOI： 10.2216/0031-8884(2008)47[334:C]2.0.CO;2

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Other Link： http://orcid.org/0000-0002-9308-4498

Authorship：Lead author   Language：English  

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Total pages：611  

車軸藻類概要、シャジクモ、イトシャジクモ 、アメリカシャジクモ、ミルフラスコモ、ハナビフラスコモ、ヒメフラスコモ、フタマタフラスコモ、キヌフラスコモ、キヌイトフラスコモ、ニッポンフラスコモ 、モリオカフラスコモ、サキボソフラスコモ 、ケフラスコモ 、カラスフラスコモ 、ナガフラスコモ、ホンフサフラスコモ 、オニフラスコモ、サイトウフラスコモ 、タナカフラスコモ

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車軸藻類

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Total pages：623  

ハダシシャジクモ、オオシャジクモ、シャジクモ、テガヌマフラスコモ、マガリフラスコモ

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イトフラスコモ、ケフラスコモ、サキボソフラスコモ、サヌキフラスコモ、ダイシフラスコモ、トゲフラスコモ、ヒナフラスコモ

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Responsible for pages：p. 4-5  

(分担執筆 p.4-5) ｢シャジクモ属｣・｢シラタマモ属｣・｢ホシツリモ属｣

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Language：Japanese   Publishing type：Rapid communication, short report, research note, etc. (scientific journal)  

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Language：Japanese   Publisher：山梨大学教育学部  

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Language：Japanese   Publisher：山梨大学教育学部  

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Language：Japanese   Publisher：山梨大学教育学部  

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Language：Japanese   Publisher：山梨大学教育学部  

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Language：English   Publishing type：Research paper, summary (international conference)   Publisher：INT PHYCOLOGICAL SOC  

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Language：Japanese   Publisher：山梨大学教育人間科学部  

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Language：English   Publisher：INT PHYCOLOGICAL SOC  

DOI： 10.2216/07-27.1

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Language：English   Publishing type：Research paper, summary (international conference)   Publisher：WILEY-BLACKWELL  

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