Updated on 2025/10/27

写真a

 
YANAGITA Kengo
 
Organization
Graduate School of Medical and Dental Sciences Specially Appointed Assistant Professor
Title
Specially Appointed Assistant Professor
Homepage
http://bb-c.gs.niigata-u.ac.jp/
External link

Degree

  • 医学博士 ( 2016.3   北里大学 )

Research Interests

  • モノクローナル抗体

  • 質量分析装置

  • プロテオーム解析

  • プロテオミクス

  • 表面プラズモン共鳴法 (SPR)

Research Areas

  • Life Science / Medical systems

  • Life Science / Nephrology

Research History (researchmap)

  • Niigata University   Specially Appointed Assistant Professor

    2019.10

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    Country：Japan

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  • Kitasato University   School of Allied Health Sciences

    2016.4 - 2019.3

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Research History

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2020.8

  • Niigata University   Graduate School of Medical and Dental Sciences   Specially Appointed Assistant Professor

    2020.1 - 2020.7

Education

  • Kitasato University   医療系研究科 博士課程

    2012.4 - 2016.3

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    Country： Japan

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  • Kitasato University   Graduate School of Medical Sciences

    2010.4 - 2012.3

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    Country： Japan

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  • Kitasato University   School of Science   化学科

    2005.4 - 2010.3

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    Country： Japan

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Professional Memberships

 

Papers

  • Association Between CKAP4 Expression and Poor Prognosis in Patients with Bladder Cancer Treated with Radical Cystectomy Reviewed International journal

    Hiroki Katsumata, Dai Koguchi, Shuhei Hirano, Anna Suzuki, Kengo Yanagita, Yuriko Shimizu, Wakana Hirono, Soichiro Shimura, Masaomi Ikeda, Hideyasu Tsumura, Daisuke Ishii, Yuichi Sato, Kazumasa Matsumoto

    Cancers   17 ( 8 )   1278 - 1278   2025.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/cancers17081278

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  • Investigating and Annotating the Human Peptidome Profile from Urine under Normal Physiological Conditions. International journal

    Amr Elguoshy, Keiko Yamamoto, Yoshitoshi Hirao, Tomohiro Uchimoto, Kengo Yanagita, Tadashi Yamamoto

    Proteomes   12 ( 3 )   2024.6

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    DOI： 10.3390/proteomes12030018

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  • Expression of S100A16 Is Associated with Biological Aggressiveness and Poor Prognosis in Patients with Bladder Cancer Who Underwent Radical Cystectomy International journal

    Hiroki Katsumata, Kazumasa Matsumoto, Kengo Yanagita, Yuriko Shimizu, Shuhei Hirano, Kazuki Kitajima, Dai Koguchi, Masaomi Ikeda, Yuichi Sato, Masatsugu Iwamura

    International Journal of Molecular Sciences   24 ( 19 )   14536 - 14536   2023.9

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    DOI： 10.3390/ijms241914536

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  • Differentiation of endogenous erythropoietin and exogenous ESAs by Western blotting. International journal

    Yukiko Yasuoka, Takashi Fukuyama, Yuichiro Izumi, Tetsuro Yamashita, Yushi Nakayama, Hideki Inoue, Kengo Yanagita, Tomomi Oshima, Taiga Yamazaki, Takayuki Uematsu, Noritada Kobayashi, Yoshitaka Shimada, Yasushi Nagaba, Masashi Mukoyama, Yuichi Sato, Jeff M Sands, Katsumasa Kawahara, Hiroshi Nonoguchi

    Heliyon   6 ( 11 )   e05389   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.heliyon.2020.e05389

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  • Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation. Reviewed International journal

    Yukiko Yasuoka, Takashi Fukuyama, Yuichiro Izumi, Yushi Nakayama, Hideki Inoue, Kengo Yanagita, Tomomi Oshima, Taiga Yamazaki, Takayuki Uematsu, Noritada Kobayashi, Yoshitaka Shimada, Yasushi Nagaba, Masashi Mukoyama, Tetsuro Yamashita, Yuichi Sato, Jeff M Sands, Katsumasa Kawahara, Hiroshi Nonoguchi

    Physiological reports   8 ( 12 )   e14485   2020.6

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    DOI： 10.14814/phy2.14485

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  • Prognostic significance of G6PD expression and localization in lung adenocarcinoma. Reviewed International journal

    Ryo Nagashio, Shota Oikawa, Kengo Yanagita, Daisuke Hagiuda, Yuki Kuchitsu, Satoshi Igawa, Katsuhiko Naoki, Yukitoshi Satoh, Masaaki Ichinoe, Yoshiki Murakumo, Makoto Saegusa, Yuichi Sato

    Biochimica et biophysica acta. Proteins and proteomics   1867 ( 1 )   38 - 46   2019.1

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    DOI： 10.1016/j.bbapap.2018.05.005

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  • Fludrocortisone stimulates erythropoietin production in the intercalated cells of the collecting ducts. Reviewed International journal

    Yukiko Yasuoka, Yuichiro Izumi, Takanori Nagai, Takashi Fukuyama, Yushi Nakayama, Hideki Inoue, Kahori Horikawa, Miho Kimura, Masayoshi Nanami, Kengo Yanagita, Tomomi Oshima, Taiga Yamazaki, Takayuki Uematsu, Rui Yamamura, Noritada Kobayashi, Yoshitaka Shimada, Yasushi Nagaba, Takeshi Nakanishi, Tetsuro Yamashita, Masashi Mukoyama, Yuichi Sato, Katsumasa Kawahara, Hiroshi Nonoguchi

    Biochemical and biophysical research communications   503 ( 4 )   3121 - 3127   2018.9

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    DOI： 10.1016/j.bbrc.2018.08.102

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  • 肺腺癌におけるNAP1L1の機能解析

    長塩 亮, 柳田 憲吾, 萩生田 大介, 朽津 有紀, 鉢村 和男, 土屋 紅緒, 一戸 昌明, 村雲 芳樹, 佐藤 雄一

    電気泳動   62 ( Suppl. )   s40 - s40   2018.8

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    Language：Japanese   Publisher：日本電気泳動学会  

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  • Cytoskeleton-Associated Protein 4 Is a Novel Serodiagnostic Marker for Lung Cancer. Reviewed International journal

    Kengo Yanagita, Ryo Nagashio, Shi-Xu Jiang, Yuki Kuchitsu, Kazuo Hachimura, Masaaki Ichinoe, Satoshi Igawa, Eriko Fukuda, Naoki Goshima, Yukitoshi Satoh, Yoshiki Murakumo, Makoto Saegusa, Yuichi Sato

    The American journal of pathology   188 ( 6 )   1328 - 1333   2018.6

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    DOI： 10.1016/j.ajpath.2018.03.007

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  • ショットガン解析により同定された膜タンパク質の有用性

    柳田 憲吾, 朽津 有紀, 一戸 昌明, 三枝 信, 土屋 紅緒, 長塩 亮, 鉢村 和男, 佐藤 雄一

    日本病理学会会誌   107 ( 1 )   415 - 415   2018.4

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    Language：Japanese   Publisher：(一社)日本病理学会  

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  • Detection of membranous proteins by the shot-gun analysis and theis utility as sero-diagnostic markers for lung cancer Reviewed

    Kengo Yanagita

    Electrophoresis Letters   62 ( 1 )   9 - 12   2018

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    Language：Japanese   Publisher：Japanese Electrophoresis Society  

    To gain novel sero-diagnostic markers for lung cancer, membranous proteins from three different histological types of lung cancer cell line were analyzed by the shot-gun proteomics method. Biotinylated membranous proteins in each lung cancer cell lines were collected and identified by the shot-gun analysis. From identified 92 membranous proteins, we focused on CD109 and CD155 proteins. Serum levels of these proteins from patients with lung cancer and healthy controls were also examined by the reverse-phase protein array (RPPA) method. Serum levels of CD109 and CD155 were significantly higher in patients with adenocarcinoma and squamous cell carcinoma of the lung than those in healthy controls. And the area under the curve of receiver-operating characteristic analysis of above tumors to healthy control of CD109 were 0.94 and 0.90, and CD155 were 0.96 and 0.77, respectively. The approach for membranous proteins using the shot-gun proteomics method is useful for acquiring novel sero-diagnostic markers.

    DOI： 10.2198/electroph.62.9

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  • Search of sero-diagnostic marker using the Reverse-phase protein array Reviewed

    Kengo Yanagita, Daisuke Hagiuda, Yuki Kuchitsu, Anna Suzuki, Wataru Inoue, Risa Matsumoto, Shinichiro Ryuge, Makoto Saegusa, Yoshiki Murakumo, Ryo Nagashio, Kazuo Hachimura, Yuichi Sato

    Electrophoresis Letters   61 ( 1 )   23 - 28   2017

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    <p>To develop sero-diagnostic markers for lung cancer, surface proteins were labeled and collected, then identified by the shotgun analysis. Surface proteins were biotinylated from three each of lung cancer cell lines and collected them. Biotinylated proteins were identified by the shotgun analysis. Expression levels of identified proteins in four typical lung cancer cell lines were confirmed by immunoblotting and immunohistochemistry. And also examined CD109 levels as a sero-diagnostic marker for lung cancer, sera from 268 lung cancer patients and 100 healthy controls were analyzed by the reverse-phase protein array (RPPA) method. Serum levels of CD109 were significantly higher in patients with adenocarcinoma and squamous cell carcinoma than in healthy controls (p < 0.0001), and the area under the curve (AUC) of receiver-operating characteristic curve (ROC) analysis was 0.93, when an optimal cut-off value of 1.13 was applied, the diagnostic sensitivity and specificity for lung cancer were 84 and 88%, respectively. In addition, because CD109 was detected even in stage I disease, serum levels of CD109 antigen should be applicable early sero-diagnostic markers for lung cancer patients discriminating from healthy controls. To our knowledge, this is the first report providing evidence that CD109 may be an early sero diagnostic marker for lung cancer.</p>

    DOI： 10.2198/electroph.61.23

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  • Detection method for membranous proteins and their utility as a sero-diagnostic markers for lung cancer Reviewed

    Kengo Yanagita, Daisuke Hagiuda, Yuki Kuchitsu, Wataru Inoue, Satoshi Igawa, Shinichiro Ryuge, Makoto Saegusa, Ryo Nagashio, Kazuo Hachimura, Yuichi Sato

    Electrophoresis Letters   61 ( 2 )   120 - 123   2017

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    <p>To develop novel sero-diagnostic markers for membranous proteins of lung cancer, surface proteins were identified in combined with various proteomic methods. Biotinylated membranous proteins in lung cancer cell lines were collected and identified by the shot-gun analysis. Expression levels of identified proteins in four different histological types of lung cancer cell line were examined by the immunoblot and immunohistochemical analyses. Serum levels of these proteins were also examined in patients with lung cancer and healthy controls by the reverse-phase protein array (RPPA) method. From identified 92 membranous proteins including CD109, CD155 and Roundabout Guidance Receptor 1 (ROBO1), we focused on the CD155 protein. Serum levels of CD155 were significantly higher in patients with adenocarcinoma and squamous cell carcinoma than in healthy controls (p < 0.0001), and the area under the curve (AUC) of receiver-operating characteristic curve (ROC) analysis was 0.87, when an optimal cut-off value of 0.31 was applied, the diagnostic sensitivity and specificity for lung cancer were 81 and 82%, respectively. To our knowledge, this is the first report providing evidence that CD155 may be a sero-diagnostic marker for non-small cell lung cancer. This methodology was shown to be useful for acquisition of novel biomarkers for membranous proteins.</p>

    DOI： 10.2198/electroph.61.120

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  • Proteome analysis using the cancer stem cell-like micropapillary adenocarcinoma cell line

    Ryo Nagashio, Daisuke Hagiuda, Yuki Kuchitsu, Kengo Yanagita, Kazuo Hachimura, Yuichi Sato

    Electrophoresis Letters   61 ( 1 )   39 - 44   2017

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    The existence of the cancer stem cell relates to prognosis and resistance to chemotherapy and radiotherapy. However, the existing cancer stem cell is very few in a cancer tissue, and it is difficult to conduct various studies. In this study, we aimed at the acquisition of the candidate marker protein to detect a cancer stem cell, we established cancer stem cell-like micropapillary adenocarcinoma cell line using reprogramming factor-induced iPS cells. Here, we compared protein profiles with cancer stem cell-like micropapillary adenocarcinoma cell line and the parent cells to discover biomarker proteins.

    DOI： 10.2198/electroph.61.39

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  • Application of markers obtained by various proteomic methods to resected non-small cell lung cancer treated with platinum-based adjuvant chemotherapy

    Sato Yuichi, Nagashio Ryo, Yanagita Kengo, Hagiuda Daisuke, Kuchitsu Yuki, Inoue Wataru

    Electrophoresis Letters   61 ( 2 )   128 - 131   2017

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    <p>We have generated several tumor markers for lung cancer using various proteomic methods including generation of monoclonal antibodies by using the random immunization method, the two-dimensional electrophoresis, and autoantibody analysis using lung cancer tissues, cell lines and patients' sera. Although the adjuvant platinum-based chemotherapy has been shown to improve survival of patients with completely resected stage II and IIIA non-small cell lung cancer (NSCLC), its effect is limited. In addition, the prior clinical examination whether this therapy is effective has not been performed. In the present study, we investigated the utility as the therapeutic effect prediction markers about the candidate marker proteins for lung cancer that we obtained previously. We review mainly on our recent study about the utility of nestin, S100A16, and myosin-9 proteins as the effect prediction factor for the adjuvant platinum-based chemotherapy.</p>

    DOI： 10.2198/electroph.61.128

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    Other Link： https://search.jamas.or.jp/link/ui/2018148640

  • Valosin-containing protein is a possible sero-diagnostic marker of psoriatic arthritis Reviewed

    Hideki Maejima, Makoto Kobayashi, Kengo Yanagita, Yuko Hamada, Ryo Nagashio, Yuichi Sato, Yasuyuki Amoh

    BIOMEDICAL RESEARCH-INDIA   28 ( 1 )   442 - 446   2017

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  • Diagnostic and prognostic significances of MUC5B and TTF-1 expressions in resected non-small cell lung cancer. Reviewed International journal

    Ryo Nagashio, Junpei Ueda, Shinichiro Ryuge, Hiroyasu Nakashima, Shi-Xu Jiang, Makoto Kobayashi, Kengo Yanagita, Ken Katono, Yukitoshi Satoh, Noriyuki Masuda, Yoshiki Murakumo, Kazuo Hachimura, Yuichi Sato

    Scientific reports   5   8649 - 8649   2015.3

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    DOI： 10.1038/srep08649

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  • Serum Anti-Gal-3 Autoantibody is a Predictive Marker of the Efficacy of Platinum-Based Chemotherapy against Pulmonary Adenocarcinoma. Reviewed International journal

    Kengo Yanagita, Ryo Nagashio, Shinichiro Ryuge, Ken Katono, Shi-Xu Jiang, Benio Tsuchiya, Hiroyasu Nakashima, Eriko Fukuda, Naoki Goshima, Makoto Saegusa, Yukitoshi Satoh, Noriyuki Masuda, Yuichi Sato

    Asian Pacific journal of cancer prevention : APJCP   16 ( 17 )   7959 - 65   2015

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    BACKGROUND: Identification of predictive markers for the efficacy of platinum-based chemotherapy is necessary to improve the quality of the life of cancer patients. MATERIALS AND METHODS: We detected proteins recognized by autoantibodies in pretreated sera from patients with lung adenocarcinoma (AC) evaluated as showing progressive disease (PD) or a partial response (PR) after cisplatin-based chemotherapy by proteomic analysis. Then, the levels of the candidate autoantibodies in the pretreated serum were validated by dot-blot analysis for 22 AC patients who received platinum-based chemotherapy, and the expression of identified proteins was immunohistochemically analyzed in 40 AC biopsy specimens. RESULTS: An autoantibody against galectin-3 (Gal-3) was detected in pretreated sera from an AC patient with PD. Serum IgG levels of anti-Gal-3 autoantibody were significantly higher in patients evaluated with PD than in those with PR and stable disease (SD) (p = 0.0084). Furthermore, pretreated biopsy specimens taken from patients evaluated as showing PD following platinum- based chemotherapy showed a tendency to have a higher positive rate of Gal-3 than those with PR and SD (p = 0.0601). CONCLUSIONS: These results suggest that serum IgG levels of anti-Gal-3 autoantibody may be useful to predict the efficacy of platinum-based chemotherapy for patients with lung AC.

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  • High expression level of preoperative serum Uroplakin III is associated with biologically aggressive bladder cancer. Reviewed International journal

    Hideyasu Tsumura, Kazumasa Matsumoto, Masaomi Ikeda, Kengo Yanagita, Shuhei Hirano, Masahiro Hagiwara, Ryo Nagashio, Tetsuo Fujita, Yuichi Sato, Masatsugu Iwamura

    Asian Pacific journal of cancer prevention : APJCP   16 ( 4 )   1539 - 43   2015

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    BACKGROUND: Uroplakins have been widely investigated as potential markers in patients with bladder cancer because these proteins are specific to the urothelium. However, the role of uroplakin proteins in bladder cancer remains unknown. In this study, preoperative serum levels of uroplakin III were measured in patients with urothelial carcinoma of the urinary bladder and examined for possible association with clinicopathological features and clinical outcomes. MATERIALS AND METHODS: This study included 52 bladder cancer patients at various stages and 28 healthy controls. Uroplakin III levels were detected in preoperative sera using an automated dot blot system and a micro-dot blot array. RESULTS: There was a significant increase in serum uroplakin III levels in patients with bladder cancer as compared to healthy controls (p<0.05). In addition, serum uroplakin III levels were associated with muscle-invasive status, high grade and lymphovascular invasion (p<0.02). Log-rank tests indicated high serum uroplakin III to be significantly associated with cancer-specific mortality. CONCLUSIONS: Determination of serum uroplakin III level could be valuable for identifying patients with biologically aggressive bladder cancer.

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  • Acquisition of tumor-associated antigens using autoantibodies in sera from patients with cancer

    Sato Yuichi, Nagashio Ryo, Kobayashi Makoto, Tsuchiya Benio, Yanagita Kengo, Hachimura Kazuo

    Electrophoresis Letters   59 ( 2 )   129 - 131   2015

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    Language：Japanese   Publisher：Japanese Electrophoresis Society  

    DOI： 10.2198/electroph.59.129

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    Other Link： https://jlc.jst.go.jp/DN/JLC/20016223408?from=CiNii

  • Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues. Reviewed

    Makoto Kobayashi, Ryo Nagashio, Shinichiro Ryuge, Yurie Murakami, Kengo Yanagita, Hiroyasu Nakashima, Toshihide Matsumoto, Shi-Xu Jiang, Makoto Saegusa, Yukitoshi Satoh, Noriyuki Masuda, Yuichi Sato

    Biomedical research (Tokyo, Japan)   35 ( 2 )   133 - 43   2014

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    DOI： 10.2220/biomedres.35.133

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  • Moesin and stress-induced phosphoprotein-1 are possible sero-diagnostic markers of psoriasis. Reviewed International journal

    Hideki Maejima, Ryo Nagashio, Kengo Yanagita, Yuko Hamada, Yasuyuki Amoh, Yuichi Sato, Kensei Katsuoka

    PloS one   9 ( 7 )   e101773   2014

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    DOI： 10.1371/journal.pone.0101773

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  • Serum expression of S100A6 is a potential detection marker in patients with urothelial carcinoma in the urinary bladder. Reviewed

    Morihiro Nishi, Kazumasa Matsumoto, Makoto Kobayashi, Kengo Yanagita, Toshihide Matsumoto, Ryo Nagashio, Daisuke Ishii, Tetsuo Fujita, Yuichi Sato, Masatsugu Iwamura

    Biomedical research (Tokyo, Japan)   35 ( 6 )   351 - 6   2014

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    DOI： 10.2220/biomedres.35.351

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  • Development of detection method for antigenic proteins that recognized by autoantibodies

    Sato Y, Nagashio R, Kobayashi M, Yanagita K, Hachimura K

    SEIBUTSU BUTSURI KAGAKU   58 ( 2 )   68 - 70   2014

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    DOI： 10.2198/sbk.58.68

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    Other Link： https://jlc.jst.go.jp/DN/JALC/10040886465?from=CiNii

  • CAXII Is a sero-diagnostic marker for lung cancer. Reviewed International journal

    Makoto Kobayashi, Toshihide Matsumoto, Shinichiro Ryuge, Kengo Yanagita, Ryo Nagashio, Yoshitaka Kawakami, Naoki Goshima, Shi-Xu Jiang, Makoto Saegusa, Akira Iyoda, Yukitoshi Satoh, Noriyuki Masuda, Yuichi Sato

    PloS one   7 ( 3 )   e33952   2012

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    DOI： 10.1371/journal.pone.0033952

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  • Utility of CAXII as a novel sero-diagnostic marker in patients with lung cancer

    Kobayashi Makoto, Matsumoto Toshihide, Nagashio Ryo, Yanagita Kengo, Ryuge Shinichiro, Shi-Xu Jiang, Saegusa Makoto, Sato Yuichi

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011   167 - 167   2011

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    Language：Japanese   Publisher：Japanese Proteomics Society (Japan Human Proteome Organisation)  

    DOI： 10.14889/jhupo.2011.0.167.0

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  • High expression of CKAP4 in sera and tissues from patients with squamous cell carcinoma of the lung

    Yanagita Kengo, Matsumoto Toshihide, Nagashio Ryo, Kobayashi Makoto, Ryuge Shinichiro, Jiang Shi-Xu, Saegusa Makoto, Sato Yuichi

    Abstracts for Annual Meeting of Japanese Proteomics Society   2011   166 - 166   2011

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    DOI： 10.14889/jhupo.2011.0.166.0

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  • 非小細胞肺癌患者血清中の抗hnRNP K 自己抗体の検出

    小林 信, 松本 俊英, 影山 泰平, 柳田 憲吾, 龍華 慎一郎, 蒋 世旭, 岡安 勲, 佐藤 雄一

    日本プロテオーム学会大会要旨集   2010   115 - 115   2010

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    Language：Japanese   Publisher：日本プロテオーム学会（日本ヒトプロテオーム機構）  

    DOI： 10.14889/jhupo.2010.0.115.1

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Books

  • 特集 電気泳動のフロンティア

    佐藤 雄一, 長塩 亮, 小林 信, 土屋 紅緒, 栁田 憲吾, 鉢村 和男

    電気泳動 

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  • 最新の電気泳動技術

    佐藤 雄一, 長塩 亮, 小林 信, 栁田 憲吾, 鉢村 和男

    生物物理化学 

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MISC

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Presentations

  • Discovery of aging markers in urinary proteins by quantitative proteomics

    Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    2025.8 

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    Event date： 2025.8

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  • Proteomics and peptidomics of urine samples during 3-day transportation

    Tomohiro Uchimoto, Keiko yamamoto, Amr Elguoshy, Kenngo Yanagita, Tadashi Yamamoto

    2025.8 

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    Event date： 2025.8

    Language：Japanese   Presentation type：Poster presentation  

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  • Acquiring urinary biomarkers that can identify the state of heart failure in diabetic patients

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    2025.8 

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    Event date： 2025.8

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Detection of age- and sex-related urinary proteins by quantitative proteomics

    Keiko Yamamoto, Kengo Yanagi, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamo

    2025.6 

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    Event date： 2025.6

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  • Advantages of quantitative proteomics in the discovery of urinary biomarkers

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro. Uchimoto, Tadashi Yamamoto

    2025.6 

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    Event date： 2025.6

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  • A Shiny Application to Create a Library from DDA Data for DIA-NN Analysis and Its Visualization

    Amr Elguoshy, Keiko Yamamoto, Kengo Yanagita, Tomohiro Uchimoto, Tadashi Yamamoto

    HUPO2024 World Congress  2024.10 

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    Event date： 2024.10

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  • Urine Bank in Biofluid Biomarker Center and Its Usability for Biomarker Discovery

    Tadashi Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Keiko Yamamoto

    HUPO2024 World Congress  2024.10 

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  • Discovery and validation of urine biomarkers for kidney nephron/site-specific damages by proteomics and immunoassay

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Chizuko Kitabayashi, Yoshio Konishi, Tadashi Yamamoto

    HUPO2024 World Congress  2024.10 

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  • Reproducibility and reliability of protein quantity analyzed by DIA quantitative proteomics for urine biomarker discovery

    Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    HUPO2024 World Congress  2024.10 

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  • Improved Protocol for Preparation of Urine Native Peptides

    Tomohiro Uchimoto, Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tadashi Yamamoto

    HUPO2024 World Congress  2024.10 

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  • Comparison of relative amounts and creatinine-corrected concentration of mass spectrometer quantitative proteomics data for urine biomarker discovery

    Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    The 67th Annual Meeting of the Japanese Society of Nephrology  2024.6 

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  • Measurement of novel renal site-specific damage biomarkers in urine of patients with kidney disease and their significance.

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Chizuko Kitabayashi, Yoshio Konishi, Tadashi Yamamoto

    The 67th Annual Meeting of the Japanese Society of Nephrology  2024.6 

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    Event date： 2024.6

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  • Proteomics based search for novel urinary kidney injury biomarkers and validation of antibody based assay system

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Chizuko Kitabayashi, Yoshio Konishi, Tadashi Yamamoto

    JPrOS2024 & JSCP20th (22nd JHUPO & 20th JSCP)  2024.6 

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    Event date： 2024.6

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  • Comparison of urine DIA proteomic data analyzed by SWATH Micro Application and DIA-NN software

    Tomohiro Uchimoto, Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tadashi Yamamoto

    JPrOS2024 & JSCP20th (22nd JHUPO & 20th JSCP)  2024.6 

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  • Limits of quantification of trace urine proteins by quantitative proteomics for urine biomarker discovery

    Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    JPrOS2024 & JSCP20th (22nd JHUPO & 20th JSCP)  2024.6 

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    Event date： 2024.6

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  • Shiny Application for Enhanced Post-Processing Analysis and Visualization of Quantitative Urine Proteomics and Peptidomics by MS and DIA-NN analysis

    Amr Elguoshy, Keiko Yamamoto, Kengo Yanagita, Tomohiro Uchimoto, Tadashi Yamamoto

    JPrOS2024 & JSCP20th (22nd JHUPO & 20th JSCP)  2024.6 

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    Event date： 2024.6

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  • 尿バンクとその利用

    栁田 憲吾

    第19回腎研究セミナー  2024.5 

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  • Discovery of urine biomarkers for early detection of kidney injuries in diabetic patients by quantitative proteomics

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    HUPO2023 World Congress  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Poster presentation  

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  • Discovery of urinary proteins correlating with HbA1c in plasma by quantitative proteomics

    K. Yamamoto, K. Yanagita, A. Elguoshy, T. Uchimoto, T. Yamamoto

    HUPO2023 World Congress  2023.9 

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    Event date： 2023.9

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  • Quantitative analysis of endogenous native peptides in urine by DIA-NN after generating a comprehensive spectral library from DDA Mascot data

    Amr Elguoshy, Keiko Yamamoto, Yoshitoshi Hirao Tomohiro Uchimoto, Shuichiro Shimada, Tadashi Yamamoto

    HUPO2023 World Congress  2023.9 

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    Event date： 2023.9

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  • Precision Analysis of Pediatric and Adult Urinary Proteins using DIA method Elucidates Fundamental of Homeostasis with Aging

    Tomohiro Uchimoto, Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tadashi Yamamoto

    HUPO2023 World Congress  2023.9 

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    Event date： 2023.9

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  • Discovery of early biomarkers for diabetic kidney disease in urine by quantitative proteomics

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Yoshitoshi Hirao, Tadashi Yamamoto

    Japanese Proteomics Society 2023  2023.7 

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    Event date： 2023.7

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Profiling of Urine Peptidome of Healthy Volunteers by clustering peptide variants for Biomarker discovery.

    Amr Elguoshy, Keiko Yamamoto, Yoshitoshi Hirao, Tomohiro Uchimoto, Kengo Yanagita, Shu-ichiro Shimada, Tadashi Yamamoto

    Japanese Proteomics Society 2023  2023.7 

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    Event date： 2023.7

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  • Discovery of urinary proteins correlating with HbA1c by quantitative proteomics

    K. Yamamoto, K. Yanagita, A. Elguoshy, T. Uchimoto, T. Yamamoto

    Japanese Proteomics Society 2023  2023.7 

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    Event date： 2023.7

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  • DIA-MS analysis for gender- and age-difference in proteome of healthy volunteer urine

    Tomohiro Uchimoto, Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tadashi Yamamoto

    Japanese Proteomics Society 2023  2023.7 

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    Event date： 2023.7

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  • Comprehensive search for markers of diabetic kidney disease by quantitative proteomics

    K. Yanagita, K. Yamamoto, A. Elguoshy, T. Uchimoto, Chizuko Kitabayashi, Yoshio Konishi, T. Yamamoto

    2023.6 

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    Event date： 2023.6

    Language：Japanese   Presentation type：Poster presentation  

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  • Discovery of biomarkers for urinary tract tissue injuries by quantitative proteomics after subtraction ofplasma proteome from urine proteome

    Keiko Yamamoto, Kengo Yanagita, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    2023.6 

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    Event date： 2023.6

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  • Search for biomarkers for early detection of renal impairment in diabetic patients

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    HUPO2022 World Congress  2022.12 

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    Event date： 2022.12

    Language：Japanese   Presentation type：Poster presentation  

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  • Endogenous peptide cluster approach for human peptidome profiling in the context of gender-age variability

    Amr Elguoshy, Keiko Yamamoto, Yoshitoshi Hirao, Tomohiro Uchimoto, Shuichiro Shimada, Tadashi Yamamoto

    HUPO2022 World Congress  2022.12 

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    Event date： 2022.12

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  • Quantitative Proteomics for Net Amount of Urinary Tract Tissue-derived Urine Proteins by Subtraction of Plasma proteins from Urine proteome

    Keiko Yamamoto, Tomohiro Uchimoto, Amr Elguoshy, Kengo Yanagita, Shuichiro Shimada, Tadashi Yamamoto

    HUPO2022 World Congress  2022.12 

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    Event date： 2022.12

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  • Quantitative Proteomics of Urine to Discover Biomarkers for Diabetes Patients

    Kengo Yanagita, Keiko Yamamoto, Amr Elguoshy, Tomohiro Uchimoto, Tadashi Yamamoto

    Japanese Proteomics Society 2022  2022.8 

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    Event date： 2022.8

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Urinary Glycoproteomic for discovery of Urine Biomarkers

    Amr Elguoshy, Keiko Yamamoto, Yoshitoshi Hirao, Tomohiro Uchimoto, Shuichiro Shimada, Tadashi Yamamoto

    Japanese Proteomics Society 2022  2022.8 

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    Event date： 2022.8

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  • Quantitative evaluation of proteins excreted from urinary tract tissues in urine by subtracting plasma proteome from urine proteome

    K. Yamamoto, K. Yanagita, A. Elguoshy, T. Uchimoto, T. Yamamoto

    Japanese Proteomics Society 2022  2022.8 

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  • 肺癌におけるCKAP4の新規腫瘍マーカーとしての有用性の検討

    栁田 憲吾, 松本 俊英, 小林 信, 龍華 慎一郎, 蒋 世旭, 岡安 勲, 佐藤 雄一

    第23回バイオサイエンスフォーラム  2010.8 

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    Event date： 2010.8

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  • 肺腺癌におけるCD239の血清診断マーカーとしての有用性

    栁田 憲吾, 朽津 有紀, 三枝 信, 長塩 亮, 土屋 紅緒, 鉢村 和男, 佐藤 雄一

    第69回日本電気泳動学会総会  2018.8 

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  • Study for the utility of membranous proteins identified by shotgun proteomics

    栁田 憲吾, 朽津 有紀, 一戸 昌明, 三枝 信, 土屋 紅緒, 長塩 亮, 鉢村 和男, 佐藤 雄一

    第107回日本病理学会総会  2018.6 

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  • 肺癌における細胞膜タンパク質同定と血清診断マーカーとしての有用性の検討

    栁田 憲吾

    第68回日本電気泳動学会総会  2017.11 

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  • CD155 antigen is a useful tissue and sero-diagnostic marker for lung cancer

    栁田 憲吾, 長塩 亮, 朽津 有紀, 松本 梨沙, 井上 航, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第106回日本病理学会総会  2017.4 

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  • Reverse-phase protein array法を用いた血清診断マーカーの探索

    栁田 憲吾

    第67回日本電気泳動学会総会  2016.8 

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  • ショットガン・プロテオミクス法による肺腺癌由来細胞の膜タンパク質の解析

    栁田 憲吾, 長塩 亮, 朽津 有紀, 松本 梨沙, 井上 航, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第105回日本病理学会総会  2016.5 

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    回日本電気泳動学会総会

    2015.9 

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  • 蛍光色素を用いたGlass array法により血清中のCKAP4抗原の測定

    栁田 憲吾, 長塩 亮, 萩生田 大介, 斎藤 慶太, 龍華 慎一郎, 蒋 世旭, 三枝 信, 佐藤 雄一

    日本プロテオーム学会2015年大会  2015.7 

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  • 肺腺癌患者血清中の膜タンパク質と反応する自己抗体を利用した分子標的薬の候補タンパク質の探索

    栁田 憲吾, 長塩 亮, 萩生田 大介, 斎藤 慶汰, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第104回日本病理学会総会  2015.4 

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  • 抗癌剤感受性予測マーカーとしての抗Galectin-3自己抗体

    栁田 憲吾, 長塩 亮, 上田 惇平, 萩生田 大介, 斉藤 慶汰, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第103回日本病理学会総会  2014.4 

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  • 肺腺癌患者におけるプラチナ製剤耐性とGalectin-3の発現との関連性について

    栁田 憲吾, 長塩 亮, 南 尚, 小林 信, 及川 将太, 杉本 愛, 上田 惇平, 萩生田 大介, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第64回日本電気泳動学会総会  2013.11 

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  • Study of Usefulness of Established Monoclonal Antibodies as a Sero Diagnostic Marker for Lung Cancer

    Kengo Yanagita, Ryo Nagashio, Benio Tsuchiya, Shinichiro Ryuge, Shi-Xu Jiang, Makoto Saegusa, Yuichi Sato

    HUPO 12th Annual World Congress  2013.9 

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  • 肺腺癌患者血清中の自己抗体を利用したGalectin-3のプラチナ製剤感受性予測マーカーとしての有用性

    栁田 憲吾, 長塩 亮, 及川 将太, 杉本 愛, 龍華 慎一郎, 中島 祐康, 土屋 紅緒, 蒋 世旭, 三枝 信, 佐藤 雄一

    第102回日本病理学会総会  2013.6 

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  • 自己抗体を用いた肺癌患者血清中のシスプラチン感受性に関わる分子の探索

    栁田 憲吾, 長塩 亮, 及川 将太, 龍華 慎一郎, 蒋 世旭, 佐藤 雄一

    第63回日本電気泳動学会総会  2012.8 

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  • 肺腺癌におけるシスプラチン感受性予測マーカーの網羅的解析

    栁田 憲吾, 長塩 亮, 松本 俊英, 南 尚, 小林 信, 村上 友利恵, 龍華 慎一郎, 蒋 世旭, 三枝 信, 鉢村 和男, 佐藤 雄一

    第101回日本病理学会総会  2012.4 

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  • 樹立抗体を用いた肺癌の血清診断マーカーの獲得

    栁田 憲吾, 長塩 亮, 鉢村 和男, 松本 俊英, 影山 泰平, 南 尚, 小林 信, 村上 友利恵, 龍華 慎一郎, 蒋 世旭, 三枝 信, 佐藤 雄一

    第62回日本電気泳動学会総会  2011.11 

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  • 肺癌患者血清中におけるCKAP4の有用性

    栁田 憲吾, 松本 俊英, 長塩 亮, 小林 信, 龍華 慎一郎, 蒋 世旭, 三枝 信, 佐藤 雄一

    第24回北里大学バイオサイエンスフォーラム  2011.8 

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  • 肺扁平上皮癌の組織・血清中において高発現するCKAP4

    栁田 憲吾, 松本 俊英, 長塩 亮, 小林 信, 龍華 慎一郎, 蒋 世旭, 三枝 信, 佐藤 雄一

    日本プロテオーム学会2011年大会  2011.7 

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  • 肺癌組織・患者血清におけるCKAP4の発現検討

    栁田 憲吾, 松本 俊英, 南 尚, 小林 信, 蒋 世旭, 三枝 信, 佐藤 雄一

    第100回日本病理学会総会  2011.4 

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Awards

  • HUPO 2022 Travel Award

    2022.11   Japanese Proteomics Society   Search for biomarkers for early detection of renal impairment in diabetic patients

    Kengo Yanagita

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  • 第68回日本電気泳動学会総会 服部賞

    2017.11   日本電気泳動学会  

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  • 優秀ポスター賞

    2015.7   日本プロテオーム学会  

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  • 修士課程優秀学位論文賞

    2011.3   北里大学  

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Research Projects

  • Discovery and Selection of Urinary Protein Biomarkers for Diabetic Kidney Injuries Detectable before Microalbuminuria

    Grant number：21K16182

    2021.4 - 2024.3

    System name：Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Research category：Grant-in-Aid for Early-Career Scientists

    Awarding organization：Japan Society for the Promotion of Science

    Yanagita Kengo

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    The number of collected urine samples increased from 75,000 at the time of this application to 150,000, and the collection is still on-going. In this study, urine samples of healthy subjects and diabetic patients who developed microalbuminuria (an index of renal impairment) were analyzed for discovery of biomarkers of early kidney injuries by quantitative proteomics and surface plasmon resonance (SPR) assay using antibodies. These assays elucidated several proteins, which increased in urine more than 1 year before development of microalbuminuria in diabetic patients.

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Other research activities

  • 令和６年度(6月期)新潟大学医学部JA新潟厚生連基金

    2024

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  • 平成26年度北里大学院生プロジェクト採用

    2014
    -
    2015

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  • 公益信託石津俊記念奨学基金

    2014
    -
    2015

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  • 平成25年度北里大学院生プロジェクト採用

    2013

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  • NPO法人つくし奨学・研究基金

    2011
    -
    2012

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