Updated on 2022/06/26

写真a

 
OKAMOTO Yuki
 
Organization
Brain Research Institute Specially Appointed Assistant
Title
Specially Appointed Assistant
External link

The Best Research Achievement in Research Career

    • 【Papers】 Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset.  2013

Degree

  • 博士(理学) ( 2013.9   総合研究大学院大学 )

  • 修士(工学) ( 2006.3   名古屋大学 )

  • 学士(工学) ( 2004.3   名古屋大学 )

Research History

  • Niigata University   Brain Research Institute   Specially Appointed Assistant

    2020.4

 

Papers

  • Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset. International journal

    Yuki Nakagami, Akiya Watakabe, Tetsuo Yamamori

    Frontiers in neural circuits   7   43 - 43   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

    We investigated the time course of the expression of several activity-dependent genes evoked by visual inputs in the primary visual cortex (V1) in adult marmosets. In order to examine the rapid time course of activity-dependent gene expression, marmosets were first monocularly inactivated by tetrodotoxin (TTX), kept in darkness for two days, and then exposed to various length of light stimulation. Activity-dependent genes including HTR1B, HTR2A, whose activity-dependency were previously reported by us, and well-known immediate early genes (IEGs), c-FOS, ZIF268, and ARC, were examined by in situ hybridization. Using this system, first, we demonstrated the ocular dominance type of gene expression pattern in V1 under this condition. IEGs were expressed in columnar patterns throughout layers II-VI of all the tested monocular marmosets. Second, we showed the regulation of HTR1B and HTR2A expressions by retinal spontaneous activity, because HTR1B and HTR2A mRNA expressions sustained a certain level regardless of visual stimulation and were inhibited by a blockade of the retinal activity with TTX. Third, IEGs dynamically changed its laminar distribution from half an hour to several hours upon a stimulus onset with the unique time course for each gene. The expression patterns of these genes were different in neurons of each layer as well. These results suggest that the regulation of each neuron in the primary visual cortex of marmosets is subjected to different regulation upon the change of activities from retina. It should be related to a highly differentiated laminar structure of marmoset visual systems, reflecting the functions of the activity-dependent gene expression in marmoset V1.

    DOI: 10.3389/fncir.2013.00043

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  • Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. International journal

    Akiya Watakabe, Shigeki Kato, Kazuto Kobayashi, Masafumi Takaji, Yuki Nakagami, Osamu Sadakane, Masanari Ohtsuka, Hiroyuki Hioki, Takeshi Kaneko, Hiroyuki Okuno, Takashi Kawashima, Haruhiko Bito, Yoshihiro Kitamura, Tetsuo Yamamori

    PloS one   7 ( 10 )   e46157   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

    We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species.

    DOI: 10.1371/journal.pone.0046157

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  • Identification of cis-acting regions that contribute to neuron-specific expression of the GAP-43 gene. International journal

    Mikio Takahashi, Yoshitaka Sato, Yuki Nakagami, Katsuhide Miyake, Shinji Iijima

    Bioscience, biotechnology, and biochemistry   70 ( 6 )   1492 - 5   2006.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    There are two transcription start sites in the growth-associated protein 43 (GAP-43) promoter, and several repressive elements have been reported in the control region. But the repressive effects have been analyzed only for the distal transcription start site. Among the repressive elements reported, we found that modulator I repressed GAP-43 gene expression from the proximal promoter in non-neuronal cells. We also found a novel stimulative element immediately downstream of modulator I.

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