2022/05/26 更新

写真a

オカモト ユキ
岡本 友貴
OKAMOTO Yuki
所属
脳研究所 特任助手
職名
特任助手
外部リンク

代表的な業績

    • 【論文】 Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset.  2013年

学位

  • 博士(理学) ( 2013年9月   総合研究大学院大学 )

  • 修士(工学) ( 2006年3月   名古屋大学 )

  • 学士(工学) ( 2004年3月   名古屋大学 )

経歴

  • 新潟大学   脳研究所   特任助手

    2020年4月 - 現在

 

論文

  • Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset. 国際誌

    Yuki Nakagami, Akiya Watakabe, Tetsuo Yamamori

    Frontiers in neural circuits   7   43 - 43   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We investigated the time course of the expression of several activity-dependent genes evoked by visual inputs in the primary visual cortex (V1) in adult marmosets. In order to examine the rapid time course of activity-dependent gene expression, marmosets were first monocularly inactivated by tetrodotoxin (TTX), kept in darkness for two days, and then exposed to various length of light stimulation. Activity-dependent genes including HTR1B, HTR2A, whose activity-dependency were previously reported by us, and well-known immediate early genes (IEGs), c-FOS, ZIF268, and ARC, were examined by in situ hybridization. Using this system, first, we demonstrated the ocular dominance type of gene expression pattern in V1 under this condition. IEGs were expressed in columnar patterns throughout layers II-VI of all the tested monocular marmosets. Second, we showed the regulation of HTR1B and HTR2A expressions by retinal spontaneous activity, because HTR1B and HTR2A mRNA expressions sustained a certain level regardless of visual stimulation and were inhibited by a blockade of the retinal activity with TTX. Third, IEGs dynamically changed its laminar distribution from half an hour to several hours upon a stimulus onset with the unique time course for each gene. The expression patterns of these genes were different in neurons of each layer as well. These results suggest that the regulation of each neuron in the primary visual cortex of marmosets is subjected to different regulation upon the change of activities from retina. It should be related to a highly differentiated laminar structure of marmoset visual systems, reflecting the functions of the activity-dependent gene expression in marmoset V1.

    DOI: 10.3389/fncir.2013.00043

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  • Visualization of cortical projection neurons with retrograde TET-off lentiviral vector. 国際誌

    Akiya Watakabe, Shigeki Kato, Kazuto Kobayashi, Masafumi Takaji, Yuki Nakagami, Osamu Sadakane, Masanari Ohtsuka, Hiroyuki Hioki, Takeshi Kaneko, Hiroyuki Okuno, Takashi Kawashima, Haruhiko Bito, Yoshihiro Kitamura, Tetsuo Yamamori

    PloS one   7 ( 10 )   e46157   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species.

    DOI: 10.1371/journal.pone.0046157

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  • Identification of cis-acting regions that contribute to neuron-specific expression of the GAP-43 gene. 国際誌

    Mikio Takahashi, Yoshitaka Sato, Yuki Nakagami, Katsuhide Miyake, Shinji Iijima

    Bioscience, biotechnology, and biochemistry   70 ( 6 )   1492 - 5   2006年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    There are two transcription start sites in the growth-associated protein 43 (GAP-43) promoter, and several repressive elements have been reported in the control region. But the repressive effects have been analyzed only for the distal transcription start site. Among the repressive elements reported, we found that modulator I repressed GAP-43 gene expression from the proximal promoter in non-neuronal cells. We also found a novel stimulative element immediately downstream of modulator I.

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