Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

This study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. Water samples were collected four times from two ponds with different vegetation types during the late spring to autumn seasons in Japan. eDNA was extracted from dissolved particles fractionated by sequential filtration through pore sizes of 200 µm, 5 µm, and 0.45 µm, followed by high-throughput amplicon sequencing targeting the plant rbcL gene. By comparing field surveys with the eDNA data, we identified 79% and 63% of plant families and genera, respectively, suggesting that pond water eDNA may reflect the surrounding terrestrial plant ecosystem. Additionally, different trends were observed in the seasonal variation of plant taxa and their composition detected in eDNA, based on particle size. This study highlights the potential of pond water eDNA to provide valuable insights into forest plant richness and seasonal dynamics, offering a novel approach for ecological monitoring.

DOI： 10.3390/biology14010062

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s10333-024-00986-6

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Other Link： https://link.springer.com/article/10.1007/s10333-024-00986-6/fulltext.html

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

Terraced paddy field landscapes in valleys have linear seminatural grasslands on surfaces adjacent to forest and paddy fields. Paddy levees are traditionally managed by regular mowing and burning, and such landscapes are important habitat for biological communities. Furthermore, these biological communities may contribute to organic matter decomposition in paddy levees. However, seminatural grasslands on paddy levees have recently decreased in Japan due to the use of herbicides. Therefore, we investigated the effects of burning and herbicide disturbances on microenvironmental factors, soil animal communities, and organic matter decomposition. Nine experimental belt transects were set up at paddy levees located on Sado Island, Japan. The burning and herbicide experiments were performed three times per year. The investigations were performed before the experiments and after the first and third experiments. The soil moisture, litter amount, pH, and electrical conductivity were measured as microenvironmental factors. Soil animals were collected and were classified into soil macrofauna and mesofauna. The organic matter decomposition rate was measured using the Bait-Lamina test. In the burning plot, the litter amount and the soil macrofauna abundance decreased after the first experiment. In the herbicide plot, the litter amount, the soil macrofauna abundance, and organic matter decomposition rate increased after the first experiment. Furthermore, with repeated herbicide spraying, the values of these parameters decreased. However, soil mesofauna abundance after the third experiment was not significantly different between all experimental plots. The change in the litter amount was different for each experiment. Furthermore, the changes in litter amount and the soil macrofauna abundance tended to coincide. Additionally, the current paper suggested that soil mesofauna community was less affected by burning and herbicide spraying compared to soil macrofauna.

DOI： 10.1007/s42729-021-00646-2

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Other Link： https://link.springer.com/article/10.1007/s42729-021-00646-2/fulltext.html

Authorship：Lead author,　Corresponding author   Language：Japanese   Publisher：The Japanese Society of Irrigation, Drainage and Rural Engineering  

<p>We investigated the influence of different levels of connectivity between forest and paddy levees on soil animals and nutrient cycles in satoyama. Three study sites were selected on Sado Island, central Japan, each of which has experienced different modifications in the transitional zone between forest and paddy levees. Twelve belt transects were set from the forest to paddy levee. In each belt transect, we collected soil animals and measured the organic matter decomposition rate. Collected soil animals were classified based on different guilds and compared between study sites. The result indicated some differences in the functional group composition of soil animals and organic matter decomposition rate in the forest and paddy levees among the study sites. At the site with high landscape connectivity, high amount of litter and high organic matter decomposition rate were observed at the forest edge. On the other hand, at the site with low landscape connectivity, relatively low densities of predator, relatively high densities of decomposers and shredders, and high organic decomposition rate were observed at paddy levees. Thus, the results suggest that even when a forest and agricultural land are adjacent to each other, the ecological function of the forest edge differs owing to differences in landscape connectivity.</p>

DOI： 10.11408/jsidre.88.I_165

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Language：Japanese  

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Language：Japanese  

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Language：Japanese   Publisher：Japan Society of Forest Planning  

DOI： 10.20659/jjfp.57.1_23

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Language：Japanese  

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Language：Japanese  

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