Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND AND AIMS: Recently, an increasing number of athletes (from recreational to professional) have chosen autologous platelet-rich plasma (PRP) therapy to treat their sports-related injuries. However, its clinical outcomes vary among individuals and are thought to be influenced mainly by the athletes' PRP quality and physical condition. Thus, for successful PRP therapy, it is crucial to evaluate platelet activities in addition to soluble bioactive factors. In previous studies, we examined male professional athletes and female elite student-athletes. To expand the findings, in this study, we focused on male recreational student-athletes and characterized their platelet energetics. METHODS: PRP was prepared from healthy male soccer club members (college student-athletes, CA) and sedentary adults of similar ages (non-athletes, NA) at rest. Plasma lactate, platelet adenosine triphosphate (ATP), and oxygen (O2) consumption levels were quantified using biochemical and bioelectrical methods. RESULTS: The body composition indices of the CA generally showed characteristics that fell between those of professional athletes and the NA. Changes in platelet lactate, ATP, or O2 consumption levels, during the 24 h incubation period did not differ significantly between the two groups. Nevertheless, the changes in ATP levels were strongly and positively correlated with those in O2 consumption only in the CA group. CONCLUSIONS: Energy generation in CAs' platelets is suggested to be more closely related to O2 consumption than that of the NA. Habitual exercise may impact platelet energetics as well as muscle cell energetics; however, further validation should be conducted with large samples to provide more insights into this hypothesis.

DOI： 10.1002/hsr2.70784

PubMed

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Language：English   Publishing type：Research paper (scientific journal)  

Plasma gel (PG) is a protein matrix prepared from platelet-poor plasma and can be utilized as a drug carrier for controlled release. We previously demonstrated its applicability as a carrier of polyphosphate. Epigallocatechin-3-gallate (EGCG) is the main flavonoid found in green tea and functions as a strong antioxidant. To explore the applicability of PG as an EGCG carrier, we examined the release of EGCG from the PG matrix using an in vitro system. Pooled platelet-poor plasma (PPP) was prepared from four healthy adult male donors, mixed with EGCG, and heated at 75 °C for 10 or 20 min to prepare the PG matrix. The PG-EGCG matrix was incubated in PBS at 37 °C, and the EGCG released into PBS was determined using spectrophotometry. The antioxidant capacity was determined based on the principle of the iodine decolorization reaction. EGCG precipitated and incorporated into the PG matrix during thermal preparation. Trypsin, used to simulate the in vivo degradation of PG, released EGCG from the PG matrix over time. The released EGCG maintained its antioxidant capacity during incubation. These results indicate that thermally prepared PG matrices can be utilized as a promising EGCG carrier in the fields of tissue engineering and regenerative medicine.

DOI： 10.3390/jfb15040098

PubMed

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Authorship：Lead author  

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND/AIM: Tumour repopulation is a major obstacle for successful cancer treatment. This study investigated whether anticancer agents contribute to tumour repopulation in TP53-mutated bile duct cancer cells. MATERIALS AND METHODS: TP53-mutated HuCCT1 and HuH28 cells were exposed to anticancer agents, and recipient cells were exposed to their conditioned media or exosomes. The effect of inhibitors and siRNA-mediated gene silencing of p38 mitogen-activated protein kinase (MAPK) and of TP53 was analyzed by cell proliferation assays and western blotting. RESULTS: Conditioned media from genotoxic agent-treated cells promoted proliferation of recipient cells (p<0.05), and this effect was abrogated by exosome inhibitors. Exosomes from gemcitabine- or cisplatin-treated cells increased cell proliferation by 1.6- to 2.2-fold (p<0.05) through p38 MAPK signalling. These effects of exosomes were inhibited by inhibition/silencing of p38 MAPK but not by TP53 silencing. CONCLUSION: Exosomal p38 MAPK plays a pivotal role in tumour repopulation in a TP53-independent manner.

DOI： 10.21873/anticanres.15533

PubMed

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND/AIM: Sorafenib is a multikinase inhibitor approved as a first-line therapy for hepatocellular carcinoma. This study examined the sorafenib resistance mechanism. MATERIALS AND METHODS: Hepatoma HepG2 cells were exposed to sorafenib, and the biological activity of the conditioned media was analyzed using cell proliferation/apoptosis assays, multiplex immunoassays, ELISA, and western blot analyses. The effect of urokinase-type plasminogen activator (uPA) inhibitors or siRNA-mediated gene silencing was examined in culture experiments and a mouse xenograft tumor model. RESULTS: Sorafenib increased uPA secretion, which was abrogated by an Akt inhibitor. The growth-inhibitory effect of sorafenib was significantly enhanced by the uPA inhibitors UK122 and amiloride. Sorafenib-induced apoptosis was increased 2.4-fold in uPA siRNA-transduced cells (p<0.05). Combined therapy with sorafenib and amiloride significantly decreased tumor volumes [mean volume: 759 mm3 (sorafenib) vs. 283 mm3 (sorafenib plus amiloride), p<0.05]. CONCLUSION: uPA may play a critical role in sorafenib resistance.

DOI： 10.21873/anticanres.14816

PubMed

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Total pages：156p   Language：Japanese

CiNii Books

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Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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Language：Japanese   Publisher：(一社)日本医療検査科学会  

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Authorship：Lead author  

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Language：Japanese   Publisher：(NPO)日本小児外科学会  

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Language：English  

CiNii Article

CiNii Books

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Other Link： http://hdl.handle.net/10191/38942

Presentation type：Oral presentation (general)  

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Presentation type：Symposium, workshop panel (nominated)  

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Presentation type：Oral presentation (general)  

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Presentation type：Oral presentation (general)  

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Presentation type：Oral presentation (general)  

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Type：Academic society, research group, etc. 

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