記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND AND AIMS: Recently, an increasing number of athletes (from recreational to professional) have chosen autologous platelet-rich plasma (PRP) therapy to treat their sports-related injuries. However, its clinical outcomes vary among individuals and are thought to be influenced mainly by the athletes' PRP quality and physical condition. Thus, for successful PRP therapy, it is crucial to evaluate platelet activities in addition to soluble bioactive factors. In previous studies, we examined male professional athletes and female elite student-athletes. To expand the findings, in this study, we focused on male recreational student-athletes and characterized their platelet energetics. METHODS: PRP was prepared from healthy male soccer club members (college student-athletes, CA) and sedentary adults of similar ages (non-athletes, NA) at rest. Plasma lactate, platelet adenosine triphosphate (ATP), and oxygen (O2) consumption levels were quantified using biochemical and bioelectrical methods. RESULTS: The body composition indices of the CA generally showed characteristics that fell between those of professional athletes and the NA. Changes in platelet lactate, ATP, or O2 consumption levels, during the 24 h incubation period did not differ significantly between the two groups. Nevertheless, the changes in ATP levels were strongly and positively correlated with those in O2 consumption only in the CA group. CONCLUSIONS: Energy generation in CAs' platelets is suggested to be more closely related to O2 consumption than that of the NA. Habitual exercise may impact platelet energetics as well as muscle cell energetics; however, further validation should be conducted with large samples to provide more insights into this hypothesis.

DOI： 10.1002/hsr2.70784

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Plasma gel (PG) is a protein matrix prepared from platelet-poor plasma and can be utilized as a drug carrier for controlled release. We previously demonstrated its applicability as a carrier of polyphosphate. Epigallocatechin-3-gallate (EGCG) is the main flavonoid found in green tea and functions as a strong antioxidant. To explore the applicability of PG as an EGCG carrier, we examined the release of EGCG from the PG matrix using an in vitro system. Pooled platelet-poor plasma (PPP) was prepared from four healthy adult male donors, mixed with EGCG, and heated at 75 °C for 10 or 20 min to prepare the PG matrix. The PG-EGCG matrix was incubated in PBS at 37 °C, and the EGCG released into PBS was determined using spectrophotometry. The antioxidant capacity was determined based on the principle of the iodine decolorization reaction. EGCG precipitated and incorporated into the PG matrix during thermal preparation. Trypsin, used to simulate the in vivo degradation of PG, released EGCG from the PG matrix over time. The released EGCG maintained its antioxidant capacity during incubation. These results indicate that thermally prepared PG matrices can be utilized as a promising EGCG carrier in the fields of tissue engineering and regenerative medicine.

DOI： 10.3390/jfb15040098

PubMed

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担当区分：筆頭著者  

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND/AIM: Tumour repopulation is a major obstacle for successful cancer treatment. This study investigated whether anticancer agents contribute to tumour repopulation in TP53-mutated bile duct cancer cells. MATERIALS AND METHODS: TP53-mutated HuCCT1 and HuH28 cells were exposed to anticancer agents, and recipient cells were exposed to their conditioned media or exosomes. The effect of inhibitors and siRNA-mediated gene silencing of p38 mitogen-activated protein kinase (MAPK) and of TP53 was analyzed by cell proliferation assays and western blotting. RESULTS: Conditioned media from genotoxic agent-treated cells promoted proliferation of recipient cells (p<0.05), and this effect was abrogated by exosome inhibitors. Exosomes from gemcitabine- or cisplatin-treated cells increased cell proliferation by 1.6- to 2.2-fold (p<0.05) through p38 MAPK signalling. These effects of exosomes were inhibited by inhibition/silencing of p38 MAPK but not by TP53 silencing. CONCLUSION: Exosomal p38 MAPK plays a pivotal role in tumour repopulation in a TP53-independent manner.

DOI： 10.21873/anticanres.15533

PubMed

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND/AIM: Sorafenib is a multikinase inhibitor approved as a first-line therapy for hepatocellular carcinoma. This study examined the sorafenib resistance mechanism. MATERIALS AND METHODS: Hepatoma HepG2 cells were exposed to sorafenib, and the biological activity of the conditioned media was analyzed using cell proliferation/apoptosis assays, multiplex immunoassays, ELISA, and western blot analyses. The effect of urokinase-type plasminogen activator (uPA) inhibitors or siRNA-mediated gene silencing was examined in culture experiments and a mouse xenograft tumor model. RESULTS: Sorafenib increased uPA secretion, which was abrogated by an Akt inhibitor. The growth-inhibitory effect of sorafenib was significantly enhanced by the uPA inhibitors UK122 and amiloride. Sorafenib-induced apoptosis was increased 2.4-fold in uPA siRNA-transduced cells (p<0.05). Combined therapy with sorafenib and amiloride significantly decreased tumor volumes [mean volume: 759 mm3 (sorafenib) vs. 283 mm3 (sorafenib plus amiloride), p<0.05]. CONCLUSION: uPA may play a critical role in sorafenib resistance.

DOI： 10.21873/anticanres.14816

PubMed

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総ページ数：156p   記述言語：日本語

CiNii Books

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担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（学術雑誌）  

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記述言語：日本語   出版者・発行元：(一社)日本医療検査科学会  

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担当区分：筆頭著者  

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記述言語：日本語   出版者・発行元：(NPO)日本小児外科学会  

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記述言語：英語   出版者・発行元：新潟大学医学部保健学科  

Background & Aims: The therapeutic potential of marrow stem cells for liver disease is currently unclear. The aim of this study was to investigate whether potentiating the chemotaxis ability of stromal cell-derived factor 1 could augment the engraftment of marrow stem cells and ameliorate tissue damage. Methods: Female mice reconstructed with β-galactosidase-positive male marrow cells were repeatedly injected with carbon tetrachloride to induce liver cirrhosis. The statuses of stromal cell-derived factor 1 and its inhibitor CD26 were examined by immunohistochemical staining, enzyme-linked immunosorbent assays and colorimetric assays. Recipients were treated with an inhibitory peptide for CD26（Diprotin A）together with granulocyte colony-stimulating factor, and the marrow-derived cells were tracked through the β-galactosidase activity or sex-determining region on the Y chromosome. Results: Stromal cell-derived factor 1 expression and CD26 activity were both significantly increased and marrow-derived hepatocytes were not observed in liver cirrhosis, suggesting that the potential of stem cell homing is impaired by liver damage. Granulocyte colony-stimulating factor only induced a few cell clusters comprising 2-6 marrow-derived hepatocytes at a late stage of liver cirrhosis. In contrast, when animals with liver cirrhosis were treated with Diprotin A after granulocyte colonystimulating factor-induced stem cell mobilization, marrow-derived hepatocytes increased to 2-4% of the total hepatocytes and both the serum albumin and hepatic hydroxyproline contents were ameliorated（p < .05）. Conclusions: Maintaining the chemotaxis ability of stromal cell-derived factor 1 may represent an effective strategy for validating stem cell therapy.

CiNii Article

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その他リンク： http://hdl.handle.net/10191/38942

会議種別：口頭発表（一般）  

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会議種別：シンポジウム・ワークショップ パネル（指名）  

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会議種別：口頭発表（一般）  

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会議種別：口頭発表（一般）  

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会議種別：口頭発表（一般）  

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種別：学会・研究会等 

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