Updated on 2024/05/19

写真a

 
SUMIYA Nobuko
 
Organization
Academic Assembly Institute of Science and Technology CHIKYU SEIBUTSU KAGAKU KEIRETU Associate Professor
Faculty of Science Department of Science Associate Professor
Title
Associate Professor
External link

Research Areas

  • Life Science / Plant molecular biology and physiology

  • Life Science / Cell biology

  • Life Science / Evolutionary biology

Research History (researchmap)

  • 東京大学大学院新領域創成科学研究科先端生命科学専攻   客員連携研究員

    2022.12

      More details

  • Niigata University   Faculty of Science Department of Science   Associate Professor

    2022.12

      More details

  • The University of Tokyo   Department of Integrated Biosciences, Graduate School of Frontier Sciences   Project Research Associate

    2022.6 - 2022.11

      More details

  • 浦和大学 非常勤講師

    2022.4 - 2023.3

      More details

  • The University of Tokyo   Department of Integrated Biosciences, Graduate School of Frontier Sciences   Postdoctoral researcher

    2021.9 - 2022.6

      More details

  • Keio University   Department of Biology   Assistant professor

    2016.9 - 2021.9

      More details

  • National Institute of Genetics   Symbiosis and Cell Evolution Laboratory   Postdoctoral researcher

    2012.1 - 2016.8

      More details

  • RIKEN   Molecular Membrane Biology Laboratory   Postdoctoral researcher

    2008.10 - 2011.12

      More details

▶ display all

Research History

  • Niigata University   Institute of Science and Technology, Academic Assembly   Associate Professor

    2022.12

  • Niigata University   Department of Science, Faculty of Science   Associate Professor

    2022.12

Education

  • The University of Tokyo   Graduate School of Frontier Science   Department of Integrated Bioscience

    2003.4 - 2008.9

      More details

  • Ochanomizu University   Faculty of Science   Department of Biology

    1999.4 - 2003.3

      More details

 

Papers

  • Nucleomorph: A Fascinating Remnant of Endosymbiosis Reviewed

    Shunnosuke Mori, Nobuko Sumiya, Sachihiro Matsunaga

    CYTOLOGIA   87 ( 3 )   203 - 208   2022.9

     More details

    Publishing type:Research paper (scientific journal)   Publisher:International Society of Cytology  

    DOI: 10.1508/cytologia.87.203

    researchmap

  • Coordination mechanism of cell and cyanelle division in the glaucophyte alga Cyanophora sudae Reviewed

    Nobuko Sumiya

    Protoplasma   259 ( 4 )   855 - 867   2022.7

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00709-021-01704-3

    researchmap

    Other Link: https://link.springer.com/article/10.1007/s00709-021-01704-3/fulltext.html

  • Cis-acting elements involved in the G2/M-phase-specific transcription of the cyclin B gene in the unicellular alga Cyanidioschyzon merolae Reviewed

    Nobuko Sumiya

    Journal of Plant Research   134 ( 6 )   1301 - 1310   2021.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media {LLC}  

    DOI: 10.1007/s10265-021-01334-z

    researchmap

  • ESCRT Machinery Mediates Cytokinetic Abscission in the Unicellular Red Alga Cyanidioschyzon merolae. Reviewed International journal

    Fumi Yagisawa, Takayuki Fujiwara, Tokiaki Takemura, Yuki Kobayashi, Nobuko Sumiya, Shin-Ya Miyagishima, Soichi Nakamura, Yuuta Imoto, Osami Misumi, Kan Tanaka, Haruko Kuroiwa, Tsuneyoshi Kuroiwa

    Frontiers in cell and developmental biology   8   169 - 169   2020

     More details

    Language:English  

    In many eukaryotes, cytokinesis proceeds in two successive steps: first, ingression of the cleavage furrow and second, abscission of the intercellular bridge. In animal cells, the actomyosin contractile ring is involved in the first step, while the endosomal sorting complex required for transport (ESCRT), which participates in various membrane fusion/fission events, mediates the second step. Intriguingly, in archaea, ESCRT is involved in cytokinesis, raising the hypothesis that the function of ESCRT in eukaryotic cytokinesis descended from the archaeal ancestor. In eukaryotes other than in animals, the roles of ESCRT in cytokinesis are poorly understood. To explore the primordial core mechanisms for eukaryotic cytokinesis, we investigated ESCRT functions in the unicellular red alga Cyanidioschyzon merolae that diverged early in eukaryotic evolution. C. merolae provides an excellent experimental system. The cell has a simple organelle composition. The genome (16.5 Mb, 5335 genes) has been completely sequenced, transformation methods are established, and the cell cycle is synchronized by a light and dark cycle. Similar to animal and fungal cells, C. merolae cells divide by furrowing at the division site followed by abscission of the intercellular bridge. However, they lack an actomyosin contractile ring. The proteins that comprise ESCRT-I-IV, the four subcomplexes of ESCRT, are partially conserved in C. merolae. Immunofluorescence of native or tagged proteins localized the homologs of the five ESCRT-III components [charged multivesicular body protein (CHMP) 1, 2, and 4-6], apoptosis-linked gene-2-interacting protein X (ALIX), the ESCRT-III adapter, and the main ESCRT-IV player vacuolar protein sorting (VPS) 4, to the intercellular bridge. In addition, ALIX was enriched around the cleavage furrow early in cytokinesis. When the ESCRT function was perturbed by expressing dominant-negative VPS4, cells with an elongated intercellular bridge accumulated-a phenotype resulting from abscission failure. Our results show that ESCRT mediates cytokinetic abscission in C. merolae. The fact that ESCRT plays a role in cytokinesis in archaea, animals, and early diverged alga C. merolae supports the hypothesis that the function of ESCRT in cytokinesis descended from archaea to a common ancestor of eukaryotes.

    DOI: 10.3389/fcell.2020.00169

    PubMed

    researchmap

  • Day/Night Separation of Oxygenic Energy Metabolism and Nuclear DNA Replication in the Unicellular Red Alga Cyanidioschyzon merolae Reviewed

    Shin-ya Miyagishima, Atsuko Era, Tomohisa Hasunuma, Mami Matsuda, Shunsuke Hirooka, Nobuko Sumiya, Akihiko Kondo, Takayuki Fujiwara

    mBio   10   e00833-19   2019.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/mBio.00833-19

    researchmap

  • Mechanism of coordination between cell and chloroplast division in unicellular algae Invited Reviewed

    Nobuko Sumiya

    Plant Morphology   30 ( 1 )   83 - 89   2018.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    researchmap

  • Hierarchal order in the formation of chloroplast division machinery in the red alga Cyanidioschyzon merolae Reviewed

    Nobuko Sumiya, Shin-ya Miyagishim

    Communicative and Integrative Biology   10 ( 2 )   e1294298   2017.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Taylor and Francis Inc.  

    Chloroplasts have evolved from a cyanobacterial endosymbiont and multiply by dividing. Chloroplast division is performed by constriction of the ring-like protein complex (the PD machinery), which forms at the division site. The PD machinery is composed of cyanobacteriadescended components such as FtsZ and eukaryote-derived proteins such as the dynamin-related protein, DRP5B. In the red alga Cyanidioschyzon merolae, FtsZ ring formation on the stromal side precedes PDR1 and DRP5B ring formation on the cytosolic side. In this study, we impaired FtsZ ring formation in C. merolae by overexpressing FtsZ just before FtsZ ring formation. As a result, PDR1 and DRP5B failed to localize at the chloroplast division site, suggesting that FtsZ ring formation is required for the PDR1 and DRP5B rings. We further found, by expressing a dominant negative form of DRP5B, that DRP5B ring formation begins on the nuclear side of the chloroplast division site. These findings provide insight into how the PD machinery forms in red algae.

    DOI: 10.1080/19420889.2017.1294298

    Scopus

    PubMed

    researchmap

  • Chloroplast division checkpoint in eukaryotic algae Reviewed

    Nobuko Sumiya, Takayuki Fujiwara, Atsuko Era, Shin-ya Miyagishima

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 47 )   E7629 - E7638   2016.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATL ACAD SCIENCES  

    Chloroplasts evolved from a cyanobacterial endosymbiont. It is believed that the synchronization of endosymbiotic and host cell division, as is commonly seen in existing algae, was a critical step in establishing the permanent organelle. Algal cells typically contain one or only a small number of chloroplasts that divide once per host cell cycle. This division is based partly on the S-phase-specific expression of nucleus-encoded proteins that constitute the chloroplast-division machinery. In this study, using the red alga Cyanidioschyzon merolae, we show that cell-cycle progression is arrested at the prophase when chloroplast division is blocked before the formation of the chloroplastdivision machinery by the overexpression of Filamenting temperaturesensitive (Fts) Z2-1 (Fts72-1), but the cell cycle progresses when chloroplast division is blocked during division-site constriction by the overexpression of either FtsZ2-1 or a dominant-negative form of dynamin-related protein 5B (DRP5B). In the cells arrested in the prophase, the increase in the cyclin B level and the migration of cyclin-dependent kinase B (CDKB) were blocked. These results suggest that chloroplast division restricts host cell-cycle progression so that the cell cycle progresses to the metaphase only when chloroplast division has commenced. Thus, chloroplast division and host cell-cycle progression are synchronized by an interactive restriction that takes place between the nucleus and the chloroplast. In addition, we observed a similar pattern of cell-cycle arrest upon the blockage of chloroplast division in the glaucophyte alga Cyanophora paradoxa, raising the possibility that the chloroplast division checkpoint contributed to the establishment of the permanent organelle.

    DOI: 10.1073/pnas.1612872113

    Web of Science

    PubMed

    researchmap

  • Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae Reviewed

    Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima

    PLANT AND CELL PHYSIOLOGY   56 ( 10 )   1962 - 1980   2015.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Nitrogen starvation is known to induce the accumulation of triacylglycerol (TAG) in many microalgae, and potential use of microalgae as a source of biofuel has been explored. However, nitrogen starvation also stops cellular growth. The expression of cyanobacterial acyl-acyl carrier protein (ACP) reductase in the unicellular red alga Cyanidioschyzon merolae chloroplasts resulted in an accumulation of TAG, which led to an increase in the number and size of lipid droplets while maintaining cellular growth. Transcriptome and metabolome analyses showed that the expression of acyl-ACP reductase altered the activities of several metabolic pathways. The activities of enzymes involved in fatty acid synthesis in chloroplasts, such as acetyl-CoA carboxylase and pyruvate dehydrogenase, were up-regulated, while pyruvate decarboxylation in mitochondria and the subsequent consumption of acetyl-CoA by the tricarboxylic acid (TCA) cycle were down-regulated. Aldehyde dehydrogenase, which oxidizes fatty aldehydes to fatty acids, was also up-regulated in the acyl-ACP reductase expresser. This activation was required for the lipid droplet accumulation and metabolic changes observed in the acyl-ACP reductase expresser. Nitrogen starvation also resulted in lipid droplet accumulation in C. merolae, while cell growth ceased as in the case of other algal species. The metabolic changes that occur upon the expression of acyl-ACP reductase are quite different from those caused by nitrogen starvation. Therefore, there should be a method for further increasing the storage lipid level while still maintaining cell growth that is different from the metabolic response to nitrogen starvation.

    DOI: 10.1093/pcp/pcv120

    Web of Science

    PubMed

    researchmap

  • A nitrogen source-dependent inducible and repressible gene expression system in the red alga Cyanidioschyzon merolae Reviewed

    Takayuki Fujiwara, Yu Kanesaki, Shunsuke Hirooka, Atsuko Era, Nobuko Sumiya, Hirofumi Yoshikawa, Kan Tanaka, Shin-Ya Miyagishima

    FRONTIERS IN PLANT SCIENCE   6   657   2015.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    The unicellular red alga Cyanidioschyzon merolae is a model organism for studying the basic biology of photosynthetic organisms. The C. merolae cell is composed of an extremely simple set of organelles. The genome is completely sequenced. Gene targeting and a heat-shock inducible gene expression system has been recently established. However, a conditional gene knockdown system has not been established, which is required for the examination of function of genes that are essential to cell viability and primary mutant defects. In the current study, we first evaluated the expression of a transgene from two chromosomal neutral loci located in the intergenic region between CMD184C and CMD185C, and a region upstream of the URA5.3 gene. There was no significant difference in expression between them and this result suggests that both may be used as neutral loci. We then designed an inducible and repressible gene expression by using promoters of nitrate-assimilation genes. The expression of nitrate-assimilation genes such as NR (nitrate reductase), NIR (nitrite reductase), and NRT (the nitrate/nitrite transporter) are reversibly regulated by their dependence on nitrogen sources. We constructed stable strains in which a cassette containing the NR, NIR or NRT promoter and sfGFP gene was inserted in a region upstream of URA5.3 and examined the efficacy of the promoters. The NR, NIR, and NRT promoters were constitutively activated in the nitrate medium, whereas their activities were extremely low in presence of ammonium. The activation of each promoter was immediately inhibited within a period of 1 h by the addition of ammonium. Thus, a conditional knockdown system in C. merolae was successfully established. The activity varies among the promoters, and each is selectable according to the expression level of a target gene estimated by RNA-sequencing. This method is applicable to defects in genes of interest in photosynthetic organism.

    DOI: 10.3389/fpls.2015.00657

    Web of Science

    Scopus

    researchmap

  • Development of a Heat-Shock Inducible Gene Expression System in the Red Alga Cyanidioschyzon merolae Reviewed

    Nobuko Sumiya, Takayuki Fujiwara, Yusuke Kobayashi, Osami Misumi, Shin-ya Miyagishima

    PLOS ONE   9 ( 10 )   e111261   2014.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    The cell of the unicellular red alga Cyanidioschyzon merolae contains a single chloroplast and mitochondrion, the division of which is tightly synchronized by a light/dark cycle. The genome content is extremely simple, with a low level of genetic redundancy, in photosynthetic eukaryotes. In addition, transient transformation and stable transformation by homologous recombination have been reported. However, for molecular genetic analyses of phenomena that are essential for cellular growth and survival, inducible gene expression/suppression systems are needed. Here, we report the development of a heat-shock inducible gene expression system in C. merolae. CMJ101C, encoding a small heat shock protein, is transcribed only when cells are exposed to an elevated temperature. Using a superfolder GFP as a reporter protein, the 200-bp upstream region of CMJ101C orf was determined to be the optimal promoter for heat-shock induction. The optimal temperature to induce expression is 50 degrees C, at which C. merolae cells are able to proliferate. At least a 30-min heat shock is required for the expression of a protein of interest and a 60-min heat shock yields the maximum level of protein expression. After the heat shock, the mRNA level decreases rapidly. As an example of the system, the expression of a dominant negative form of chloroplast division DRP5B protein, which has a mutation in the GTPase domain, was induced. Expression of the dominant negative DRP5B resulted in the appearance of aberrant-shaped cells in which two daughter chloroplasts and the cells are still connected by a small DRP5B positive tube-like structure. This result suggests that the dominant negative DRP5B inhibited the final scission of the chloroplast division site, but not the earlier stages of division site constriction. It is also suggested that cell cycle progression is not arrested by the impairment of chloroplast division at the final stage.

    DOI: 10.1371/journal.pone.0111261

    Web of Science

    PubMed

    researchmap

  • Translation-independent circadian control of the cell cycle in a unicellular photosynthetic eukaryote Reviewed

    Shin-ya Miyagishima, Takayuki Fujiwara, Nobuko Sumiya, Shunsuke Hirooka, Akihiko Nakano, Yukihiro Kabeya, Mami Nakamura

    NATURE COMMUNICATIONS   5   3807   2014.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Circadian rhythms of cell division have been observed in several lineages of eukaryotes, especially photosynthetic unicellular eukaryotes. However, the mechanism underlying the circadian regulation of the cell cycle and the nature of the advantage conferred remain unknown. Here, using the unicellular red alga Cyanidioschyzon merolae, we show that the G1/S regulator RBR-E2F-DP complex links the G1/S transition to circadian rhythms. Time-dependent E2F phosphorylation promotes the G1/S transition during subjective night and this phosphorylation event occurs independently of cell cycle progression, even under continuous dark or when cytosolic translation is inhibited. Constitutive expression of a phospho-mimic of E2F or depletion of RBR unlinks cell cycle progression from circadian rhythms. These transgenic lines are exposed to higher oxidative stress than the wild type. Circadian inhibition of cell cycle progression during the daytime by RBR-E2F-DP pathway likely protects cells from photosynthetic oxidative stress by temporally compartmentalizing photosynthesis and cell cycle progression.

    DOI: 10.1038/ncomms4807

    Web of Science

    PubMed

    researchmap

  • Localization and evolution of septins in algae Reviewed

    Tomokazu Yamazaki, Satomi Owari, Shuhei Ota, Nobuko Sumiya, Maki Yamamoto, Koichi Watanabe, Tamotsu Nagumo, Shinichi Miyamura, Shigeyuki Kawano

    Plant Journal   74 ( 4 )   605 - 614   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Septins are a group of GTP-binding proteins that are multi-functional, with a well-known role in cytokinesis in animals and fungi. Although the functions of septins have been thoroughly studied in opisthokonts (fungi and animals), the function and evolution of plant/algal septins are not as well characterized. Here we describe septin localization and expression in the green algae Nannochloris bacillaris and Marvania geminata. The present data suggest that septins localize at the division site when cytokinesis occurs. In addition, we show that septin homologs may be found only in green algae, but not in other major plant lineages, such as land plants, red algae and glaucophytes. We also found other septin homolog-possessing organisms among the diatoms, Rhizaria and cryptomonad/haptophyte lineages. Our study reveals the potential role of algal septins in cytokinesis and/or cell elongation, and confirms that septin genes appear to have been lost in the Plantae lineage, except in some green algae. © 2013 John Wiley &amp
    Sons Ltd.

    DOI: 10.1111/tpj.12147

    Scopus

    PubMed

    researchmap

  • ROLE OF MULTIPLE FTSZ RINGS IN CHLOROPLAST DIVISION UNDER OLIGOTROPHIC AND EUTROPHIC CONDITIONS IN THE UNICELLULAR GREEN ALGA NANNOCHLORIS BACILLARIS (CHLOROPHYTA, TREBOUXIOPHYCEAE) Reviewed

    Nobuko Sumiya, Satomi Owari, Koichi Watanabe, Shigeyuki Kawano

    JOURNAL OF PHYCOLOGY   48 ( 5 )   1187 - 1196   2012.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    Chloroplasts of the unicellular green alga Nannochloris bacillaris Naumann cultured under nutrient-enriched conditions have multiple rings of FtsZ, a prokaryote-derived chloroplast division protein. We previously reported that synthesis of excess chloroplast DNA and formation of multiple FtsZ rings occur simultaneously. To clarify the role of multiple FtsZ rings in chloroplast division, we investigated chloroplast DNA synthesis and ring formation in cells cultured under various culture conditions. Cells transferred from a nutrient-enriched medium to an inorganic medium in the light showed a drop in cell division rate, a reduction in chloroplast DNA content, and changes in the shape of chloroplast nucleoids as cells divided. We then examined DNA synthesis by immunodetecting BrdU incorporated into DNA strands using the anti-BrdU antibody. BrdU-labeled nuclei were clearly observed in cells 48 h after transfer into the inorganic medium, while only weak punctate signals were visible in the chloroplasts. In parallel, the number of FtsZ rings decreased from 6 to only 1. When the cells were transferred from an inorganic medium to a nutrient-enriched medium, the number of cells increased only slightly in the first 12 h after transfer; after this time, however, they started to divide more quickly and increased exponentially. Chloroplast nucleoids changed from punctate to rod-like structures, and active chloroplast DNA synthesis and FtsZ ring formation were observed. On the basis of our results, we conclude that multiple FtsZ ring assembly and chloroplast DNA duplication under nutrient-rich conditions facilitate chloroplast division after transfer to oligotrophic conditions without further duplication of chloroplast DNA and formation of new FtsZ rings.

    DOI: 10.1111/j.1529-8817.2012.01204.x

    Web of Science

    researchmap

  • Lipid Droplets of Bacteria, Algae and Fungi and a Relationship between their Contents and Genome Sizes as Revealed by BODIPY and DAPI Staining Reviewed

    Tsuneyoshi Kuroiwa, Mio Ohnuma, Yuuta Imoto, Osami Misumi, Takayuki Fujiwara, Shin-ya Miyagishima, Nobuko Sumiya, Haruko Kuroiwa

    CYTOLOGIA   77 ( 3 )   289 - 299   2012.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:UNIV TOKYO CYTOLOGIA  

    We have developed a convenient method to visualize triacylglycerol-filled lipid droplets (LDs) in some species of bacteria, algae and fungi by staining with borondipyrromethene difluoride (BODIPY). When BODIPY was excited by blue light, LDs emitted green fluorescence, which was distinguished easily from the red autofluorescence of chloroplasts. This makes BODIPY staining suitable for the identification of small amounts of LDs, especially in plants. We first ensured that in Chlamydomonas reinhardtii cells growing in nitrogen-replete (+N) and -deficient (-N) media, the spots of BODIPY-stained LDs coincided with those of Nile Red-stained LDs. In addition, it was shown that the LD content per cell in N-starved cells was 200-fold higher than those of the control (+N) using a video-intensified microscope photoncounting system (VIMPCS). BODIPY staining was applied to visualize LD in bacteria, algae and fungi, and included those algae regarded as non-oleaginous. We identified LD spots in unicellular and multicellular bacteria and eukaryotes, namely Cyanidioschyzon merolae, Cyanidium caldarium delta, Chlamydomonas reinhardtii, Klebsormidium nitens and Penicillium sp., but not in Anabaena flos-aquae. We also examined the relationship between the contents of LDs and the genome size in the algae and fungi using VIMPCS but were unable to find a strong relationship between genome size and production of LDs. Finally, the location of LDs was considered in relation to organelles including the endoplasmic reticulum and chloroplasts, which are related to the formation of LDs.

    DOI: 10.1508/cytologia.77.289

    Web of Science

    researchmap

  • Chloroplast Division and Differentially Regulated Expression of FtsZ1 and FtsZ2 in the Synchronous Culture of Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae) Reviewed

    Nobuko Sumiya, Tomokazu Yamazaki, Satomi Owari, Maki Yamamoto, Koichi Watanabe, Shigeyuki Kawano

    CYTOLOGIA   77 ( 1 )   59 - 66   2012.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:UNIV TOKYO CYTOLOGIA  

    In plants, the prokaryote-derived chloroplast division protein FtsZ is divided into the FtsZ1 and FtsZ2 families. To clarify the relationship between FtsZ1 and FIsZ2 expression and chloroplast division in the unicellular green alga Nannochloris bacillaris, we compared FtsZ1 and FriZ2 expression in alga cultured in 2 nutritionally different media. Cells grown in a rich medium had 1 FtsZ ring throughout the cell division cycle, whereas cells in an inorganic medium showed only 1 FtsZ ring during the chloroplast division phase. Both FtsZ1 and FtsZ2 mRNA levels in the rich medium were higher than those in the inorganic medium. However, a much larger difference in the amount of transcripts between cells cultured in the rich and in the inorganic media was observed in FtsZ2 than that in FtsZ1, suggesting different gene regulatory mechanisms between the FtsZ genes. N. bacillaris cells cultured in inorganic medium showed cell division photoperiodicity, whereas cells cultured in organic media proliferated continuously under a light/dark cycle. Although FtsZ1 was expressed constantly throughout the chloroplast division cycle in the rich medium, the increase in FisZ1 mRNA level was simultaneous with chloroplast division under a light/dark cycle in the inorganic medium. FtsZ2 was expressed constantly in both media. The control of chloroplast division by regulating FtsZ ring formation is discussed.

    DOI: 10.1508/cytologia.77.59

    Web of Science

    researchmap

  • MULTIPLE FtsZ RING FORMATION AND REDUPLICATED CHLOROPLAST DNA IN NANNOCHLORIS BACILLARIS (CHLOROPHYTA, TREBOUXIOPHYCEAE) UNDER PHOSPHATE-ENRICHED CULTURE Reviewed

    Nobuko Sumiya, Aiko Hirata, Shigeyuki Kawano

    JOURNAL OF PHYCOLOGY   44 ( 6 )   1476 - 1489   2008.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    We examined the effects of phosphate enrichment on chloroplasts of the unicellular green alga Nannochloris bacillaris Naumann. The doubling time of cells was similar in phosphate-limited (no beta-glycerophosphate) and phosphate-enriched (2 mM beta-glycerophosphate) media. The lengths of cells and chloroplasts were similar, regardless of phosphate concentration. The relationship between the ring formation of the prokaryote-derived chloroplast division protein FtsZ and phosphate concentration was examined using indirect fluorescent antibody staining. The number of FtsZ rings increased as the phosphate concentration of the medium increased. Multiple FtsZ rings were formed in cells in phosphate-enriched medium; up to six FtsZ rings per chloroplast were observed. The number of FtsZ rings increased as the chloroplast grew. The FtsZ ring located near the center of the chloroplast had the strongest fluorescence. The FtsZ ring at the relative center of all FtsZ rings was used for division. Plastid division rings did not multiply in phosphate-enriched culture. The chloroplast DNA content was 2.3 times greater in phosphate-enriched than in phosphate-limited culture and decreased in cells cultured in phosphate-enriched medium containing 5-fluorodeoxyuridine (FdUr). In the presence of FdUr, only one FtsZ ring formed, even under phosphate enrichment. This finding suggests that excessive chloroplast DNA replication induces multiple FtsZ ring formation in phosphate-enriched culture. We propose a multiple FtsZ ring formation model under phosphate enrichment.

    DOI: 10.1111/j.1529-8817.2008.00589.x

    Web of Science

    CiNii Article

    researchmap

  • Molecular divergence and characterization of two chloroplast division genes, Ftsz1 and Ftsz2, in the unicellular green alga Nannochloris bacillaris (Chlorophyta) Reviewed

    T Koide, T Yamazaki, M Yamamoto, M Fujishita, H Nomura, Y Moriyama, N Sumiya, S Matsunaga, W Sakamoto, S Kawano

    JOURNAL OF PHYCOLOGY   40 ( 3 )   546 - 556   2004.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL PUBLISHING INC  

    Two FtsZ paralogues (NbFtsZ1 and NbFtsZ2) were isolated from the unicellular green alga Nannochloris bacillaris Naumann. These sequences encoded proteins of 435 and 439 amino acids with tubulin signature motifs (GGGTG[T/S]G), which are important for GTP binding activity. NbFtsZ1 and NbFtsZ2 had four and three introns, respectively, and two different putative core promoters; a TATA box (TATAAAA) and an initiator element (CCCAGG) were located 40 bp and 80 bp upstream of the coding regions of NbFtsZ1 and NbFtsZ2, respectively. Southern blot hybridization and contour-clamped homogeneous electric field electrophoresis showed that N. bacillaris contained at least one copy of each gene and that NbFtsZ1 was located on chromosome 5 and NbFtsZ2 on chromosome 3 or 4. Phylogenetically, NbFtsZ1 and NbFtsZ2 belong to the vascular plant protein families FtsZ1 and FtsZ2, respectively. The FtsZ1 proteins do not contain carboxy-terminal consensus sequences, whereas all FtsZ2 proteins possess the consensus sequence (I/V)PxFL(R/K)(K/R)(K/R). Our study has shown that NbFtsZ2 possesses a similar consensus sequence (VPDFLRRK), whereas NbFtsZ1 does not, further supporting their classification as FtsZ2 and FtsZ1. Escherichia coli ftsZ mutants transformed with cloned NbFtsZ1, and NbFtsZ2 cDNAs were restored for the capacity to divide by binary fission, suggesting that the proteins retained the ability to function in the bacterium. An anti-NbFtsZ2 antibody specifically recognized a single protein band of approximately 51 kDa on an immunoblot of N. bacillaris cellular proteins. Immunostaining of the algal cells with this antibody produced an intense fluorescent signal as a ring near the middle of the cell, which corresponded to the chloroplast division site.

    DOI: 10.1111/j.1529-8817.2004.03105.x

    Web of Science

    researchmap

▶ display all

Books

  • Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology

    Sumiya N, Miyagishima S( Role: Contributor ,  Chap. 21 Metabolic Engineering of Cyanidioschyzon merolae)

    Springer, Singapore  2017 

     More details

MISC

  • 単細胞藻の細胞と葉緑体の分裂の相互制御 Invited

    墨谷 暢子

    アグリバイオ   3 ( 3 )   49 - 53   2019.3

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

    researchmap

  • トレボキシア藻綱の分裂様式:細胞伸長の方向を決める壁合成とセプチンホモログの局在

    尾張智美, 墨谷暢子, 山本真紀, 河野重行

    日本植物学会大会研究発表記録   72nd   151   2008.9

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • トレボキシア藻綱Nannochloris bacillarisとMarvania geminataの細胞分裂とセプチンホモログの局在

    尾張智美, 山崎誠和, 墨谷暢子, 山本真紀, 河野重行

    藻類   56 ( 1 )   93   2008.3

     More details

    Language:Japanese  

    J-GLOBAL

    researchmap

  • Induction of multiple FtsZ rings in the micro green alga, Nannochloris bacillaris with phosphate enriched medium

    SUMIYA Nobuko, YAMAMOTO Maki, HIRATA Aiko, KAWANO Shigeyuki

    119 ( 0 )   123 - 123   2006.12

     More details

Presentations

  • 単細胞藻における細胞と葉緑体の分裂の協調機構 Invited

    墨谷暢子

    第63回新潟生化学懇話会  2023.7 

     More details

    Event date: 2023.7

    Presentation type:Oral presentation (invited, special)  

    researchmap

  • 単細胞藻類における細胞と葉緑体の分裂の協調 Invited

    インターキャンパスセミナー in Niigata  2023.11 

     More details

  • 単細胞紅藻Cyanidioschyzon merolaeにおけるG2/M期移行

    墨谷 暢子

    日本植物学会第83 回大会  2019.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 単細胞紅藻Cyanidioschyzon merolaeにおけるR1R2R3 Myb の機能解析

    墨谷 暢子

    日本植物形態学会第 31 回大会  2019.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 灰色藻Cyanophora sudaeの細胞周期進行と葉緑体分裂

    墨谷 暢子

    日本植物学会第82回大会  2018.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 単細胞藻類における細胞分裂と葉緑体分裂の協調機構

    墨谷 暢子

    日本植物形態学会第29回大会  2017.9 

     More details

  • 真核藻類における栄養環境と葉緑体分裂制御 Invited

    墨谷暢子

    河野重行教授退職記念シンポジウム  2017.5 

     More details

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    researchmap

  • Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae International conference

    Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima

    6th Asian Symposium on Plant Lipids  2015.12 

     More details

    Language:English   Presentation type:Poster presentation  

    researchmap

  • 真核藻類における葉緑体分裂チェックポイントの解析

    墨谷暢子, 藤原崇之, 恵良厚子, 宮城島進也

    日本植物学会第79回大会  2015.9 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 真核藻類における葉緑体分裂チェックポイントの解析

    墨谷暢子, 藤原崇之, 恵良厚子, 宮城島進也

    日本植物形態学会第 27 回大会  2015.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 単細胞紅藻の細胞周期進行における葉緑体分裂チェックポイント

    墨谷暢子, 藤原崇之, 恵良厚子, 宮城島進也

    第67回日本細胞生物学会大会  2015.7 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 単細胞紅藻Cyanidioschyzon merolaeにおけるシアノバクテリアAcyl-ACP reductaseの発現はトリアシルグリセロールの量を増加させる

    墨谷 暢子, 河瀬 泰子, 早川 准平, 松田 真実, 中村 真心, 恵良 厚子, 田中 寛, 近藤 明彦, 蓮沼 誠久, 今村 壮輔, 宮城島 進也

    第27回植物脂質シンポジウム  2014.11 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 単細胞藻類の細胞周期進行における葉緑体分裂チェックポイント

    墨谷暢子, 藤原崇之, 恵良厚子, 宮城島進也

    日本植物学会 第78回大会  2014.9 

     More details

    Language:Japanese   Presentation type:Oral presentation (general)  

    researchmap

  • 単細胞紅藻の細胞周期における葉緑体分裂チェックポイント

    墨谷暢子, 藤原崇之, 恵良厚子, 宮城島進也

    日本植物形態学会第26回大会  2014.9 

     More details

    Language:Japanese   Presentation type:Poster presentation  

    researchmap

  • 単細胞紅藻Cyanidioschyzon merolaeの遺伝子発現誘導系の確立と応用

    墨谷 暢子, 小林 優介, 三角 修己, 宮城島 進也

    日本植物学会第77回大会  2013.9 

     More details

    Presentation type:Oral presentation (general)  

    Venue:札幌  

    国立遺伝学研究所以外との共同発表あり

    researchmap

  • 細胞周期の進行と連携した葉緑体とミトコンドリア分裂の制御機構の解析

    Fujiwara, T, Sumiya, N, Miyagishima, S

    日本植物学会第77回大会  2013.9 

     More details

    Presentation type:Oral presentation (general)  

    Venue:札幌  

    researchmap

  • 単細胞紅藻Cyanidioschyzon merolaeの遺伝子発現誘導系の開発

    墨谷 暢子, 小林 優介, 三角 修己, 宮城島 進也

    日本植物形態学会第 25 回大会  2013.9 

     More details

    Presentation type:Poster presentation  

    Venue:札幌  

    国立遺伝学研究所以外との共同発表あり

    researchmap

  • 単細胞紅藻Cyanidioschyzon merolaeの遺伝子発現誘導系の開発

    墨谷 暢子, 小林 優介, 三角 修己, 宮城島 進也

    日本植物学会第76回大会  2012.9 

     More details

    Presentation type:Poster presentation  

    Venue:姫路  

    国立遺伝学研究所以外との共同発表あり

    researchmap

▶ display all

Awards

  • The Young Scientist Award for Plant Morphology

    2017.9   The Japanese Society of Plant Morphology  

    Sumiya Nobuko

     More details

  • Hirase Award

    2017.9   The Japanese Society of Plant Morphology  

    Sumiya Nobuko

     More details

  • 第27回植物脂質シンポジウム ベストポスター賞

    2014.11  

    墨谷 暢子

     More details

Research Projects

  • 真核藻の細胞と葉緑体の分裂協調機構の分子細胞形態学的解析

    Grant number:23K05827

    2023.4 - 2026.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    墨谷 暢子

      More details

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    researchmap

  • 真核藻類の葉緑体分裂開始による細胞周期のチェックポイントの解除機構とその普遍性

    2017.4 - 2020.3

    System name:科学研究費補助金(若手研究(B))

    Awarding organization:文部科学省

    墨谷 暢子

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

  • 出芽酵母RabカスケードにおけるRab活性変化の視覚化

    Grant number:21870050

    2009 - 2010

    System name:科学研究費補助金(若手研究(スタートアップ))

    Research category:若手研究(スタートアップ)

    Awarding organization:文部科学省

    墨谷暢子

      More details

    Authorship:Principal investigator  Grant type:Competitive

    researchmap

 

Teaching Experience (researchmap)

  • 理学基礎演習(自然環境科学プログラム)

    2024.6

     More details

  • 植物生理学演習

    2024.4

     More details

  • 生物学実習III

    2024.4

     More details

  • 課題研究I(生物学)

    2024.4

     More details

  • 環境生物学演習

    2023.10

     More details

  • 自然環境科学研究演習

    2023.10

     More details

  • 自然環境科学実験B1

    2023.10

     More details

  • 生物学総合演習

    2023.10

     More details

  • 進化生物学特論III

    2023.10

     More details

  • 自然環境科学総論

    2023.10

     More details

  • 生物学基礎B

    2023.6

     More details

  • 自然環境科学実験B2

    2023.6

     More details

  • 自然科学総論V

    2023.6

     More details

  • 課題研究(自然環境)B

    2023.4

     More details

  • 生物学基礎実習b

    2023.1

     More details

  • 生命の倫理

    2022.9
    -
    2023.3

     More details

  • 自然科学の成立と発展

    2022.4
    -
    2022.9

     More details

  • 生物学実験A

    2019
    -
    2021
    Institution name:慶應義塾大学通信教育課程

     More details

  • 生物学I(実験を含む)

    2017
    -
    2021

     More details

  • 生物学実験B

    2017
    -
    2018
    Institution name:慶應義塾大学通信教育課程

     More details

  • 生物学II(実験を含む)

    2016
    -
    2020

     More details

▶ display all

Teaching Experience

  • 自然環境科学研究演習

    2023
    Institution name:新潟大学

  • 課題研究(自然環境)B

    2023
    Institution name:新潟大学

  • 共生進化学特論

    2023
    Institution name:新潟大学

  • 生物学総合演習

    2023
    Institution name:新潟大学

  • 安全教育

    2023
    Institution name:新潟大学

  • 生物学基礎実習b

    2023
    Institution name:新潟大学

  • 理学スタディ・スキルズ

    2023
    Institution name:新潟大学

  • 環境生物学演習

    2023
    Institution name:新潟大学

  • 自然環境科学実験B2

    2023
    Institution name:新潟大学

  • 自然環境科学実験B1

    2023
    Institution name:新潟大学

  • 自然環境科学総論

    2023
    Institution name:新潟大学

  • 課題研究II(生物学)

    2023
    Institution name:新潟大学

  • 課題研究I(生物学)

    2023
    Institution name:新潟大学

  • 生物学総合演習

    2023
    Institution name:新潟大学

  • 生物学基礎B

    2023
    Institution name:新潟大学

  • 自然科学総論V

    2023
    Institution name:新潟大学

  • 進化生物学特論III

    2023
    Institution name:新潟大学

▶ display all