Updated on 2025/11/08

写真a

 
JOH Toshio
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Professor
Faculty of Agriculture Department of Agriculture Professor
Title
Professor
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Degree

  • Doctor (Agriculture) ( 1993.3   Kyushu University )

Research Areas

  • Life Science / Food sciences

Research History (researchmap)

  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2017.4

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  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry Applied Biological Chemistry   Professor

    2016.4 - 2017.3

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  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2016.3

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  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2016.3

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  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1999.4 - 2007.3

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  • Niigata University   Faculty of Agriculture   Assistant

    1993.4 - 1999.3

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Research History

  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2017.4

  • Niigata University   Abolition organization Applied Biological Chemistry   Professor

    2016.4 - 2017.3

  • Niigata University   Faculty of Agriculture Department of Applied Biological Chemistry   Associate Professor

    2007.4 - 2016.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2016.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2007.4 - 2016.3

  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1999.4 - 2007.3

  • Niigata University   Faculty of Agriculture   Research Assistant

    1993.4 - 1999.3

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Papers

  • A simple genotyping method for <i>RIM15</i> gene polymorphisms in sake yeast strains

    Takashi Kuribayashi, Nanako Asahi, Hina Satone, Jumpei Tanaka, Masamichi Sugawara, Keigo Sato, Yoshihito Nabekura, Toshio Joh, Toshio Aoki

    Mycoscience   2025

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    Publishing type:Research paper (scientific journal)   Publisher:The Mycological Society of Japan  

    DOI: 10.47371/mycosci.2024.12.001

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  • Genotypic analysis of the FAS2-F1279Y (3836T>A) polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains. Reviewed

    Takashi Kuribayashi, Toshiki Sakurai, Akira Hatakeyama, Toshio Joh, Mitsuoki Kaneoke

    The Journal of general and applied microbiology   68 ( 5 )   248 - 252   2023.1

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    In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, which is synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant with a single nucleotide substitution in the alpha subunit of fatty acid synthase (FAS2) gene (F1279Y; 3836T>A) that produced large amounts of ethyl caprylate. Here, we designed two primer sets (P1/P2 and P3/P4) with mismatches that incorporate restriction sites for the enzymes NdeI and SspI, respectively and developed an easy and rapid polymerase chain reaction-restriction fragment length polymorphism assay to identify yeasts harboring the FAS2-F1279Y mutation associated with high ethyl caprylate productivity.

    DOI: 10.2323/jgam.2022.05.001

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  • Black rice bran intake reduces phosphorylated tau levels and enhances insulin signaling in the brain of aged normal mice. Reviewed International journal

    Takashi Hara, Hanae Toyama, Yumi Ohata, Takeshi Ikeuchi, Sumiko Nakamura, Toshio Joh, Ken'ichi Ohtsubo

    Bioscience, biotechnology, and biochemistry   86 ( 11 )   1570 - 1575   2022.10

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    This study reports that black rice bran (BRB) intake for 50-52 consecutive weeks (∼12 months) reduces tau phosphorylation with a concomitant activation of insulin signaling and subsequent PI3K/AKT pathway in the brain of aged normal mice. BRB holds promise for preventing the formation of neurofibrillary tangles consisting of hyperphosphorylated tau, a pathological hallmark of Alzheimer's disease.

    DOI: 10.1093/bbb/zbac125

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  • Heat-killed Lactobacillus paracasei K71 isolated from Sakekasu (sake lees) enhances Th1 response, NK cell cytotoxicity and fecal IgA content in normal BALB/c mice. Reviewed

    Takashi Hara, Akira Igarashi, Takuya Teradaira, Toshio Joh

    Food Science and Technology Research   28 ( 4 )   335 - 341   2022.7

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  • Decomposition and effect as prebiotics of inulin in Jerusalem artichoke tubers during storage Reviewed

    Morita Akira, Hara Takashi, Joh Toshio

    Nippon Shokuhin Kagaku Kogaku Kaishi   69 ( 6 )   313 - 319   2022.6

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    In order to reveal the effect of normal temperature storage on the decomposition of inulin, Jerusalem artichoke tubers were stored at 20 °C and 5 °C for 90 days, and changes in the content and molecular weight of inulin were investigated. The rate of decrease on day 90 was 37.4 % at 20 °C and 31.1 % at 5 °C. Low-molecular-weight inulin with a chain length of 3 to 4 units increased significantly during storage at 20 °C. Inulin was divided into low- and high-molecular weight fractions by gel filtration, and the growth effect on <i>Bifidobacterium</i> spp. was examined. The low-molecular weight fraction showed a strong effect. In addition, an extract of Jerusalem artichoke stored at 20 °C had greater growth effect on <i>Bifidobacterium</i> spp. than the one immediately after harvest.

    DOI: 10.3136/nskkk.69.313

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    Other Link: https://www.jstage.jst.go.jp/article/nskkk/69/6/69_313/_pdf

  • Isolation and brewing properties of a sake yeast mutant with high ethyl caprylate productivity Reviewed

    Takashi Kuribayashi, Akira Hatakeyama, Jun Yarimizu, Keigo Arimoto, Mitsuoki Kaneoke, Yuji Tasaki, Takashi Hara, Toshio Joh

    Food Science and Technology Research   28 ( 3 )   217 - 224   2022.5

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    DOI: 10.3136/fstr.fstr-d-21-00308

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  • Effects of the method of Moromi-filtration on flavor components and free fatty acids of sake Reviewed

    Nagai, Y., Suzuki, T.

    115 ( 12 )   769 - 776   2020.12

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    Other Link: http://id.ndl.go.jp/bib/031200436

  • Screening and characterization of a house yeast strain for sake brewing using the loop-mediated isothermal amplification method Reviewed

    Hatakeyama, A., Watanabe, Y., Arimoto, K., Kuribayashi, T., Hara, T., Joh, T.

    115 ( 9 )   537 - 544   2020.9

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    Other Link: http://id.ndl.go.jp/bib/030663322

  • Antioxidant Activity and Ferulic Acid Content of Brown Rice Koji and Brown Rice Shio-Koji Prepared Using Three Species of Koji Molds Reviewed

    Han Baoxiang, Hara Takashi, Joh Toshio

    Nippon Shokuhin Kagaku Kogaku Kaishi   67 ( 7 )   236 - 241   2020.7

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    Language:Japanese   Publisher:Japanese Society for Food Science and Technology  

    For the purpose of improving rice functionality by effectively utilizing rice bran, brown rice (BR) koji and BR shio-koji were prepared using three species of koji molds and their antioxidant activities were investigated. The antioxidant activities of the three BR koji were higher than that of BR, and that of the BR koji of <i>Aspergillus luchuensis </i>(AL) was the highest. AL-BR koji contained about 250 times more ferulic acid, an antioxidant component, than BR, and the used AL showed high feruloyl esterase (FAE) activity. However, the ferulic acid content of AL-BR shio-koji was lower than that of AL-BR koji, and the FAE of AL was inhibited by NaCl.

    DOI: 10.3136/nskkk.67.236

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    Other Link: https://www.jstage.jst.go.jp/article/nskkk/67/7/67_236/_pdf

  • Genotypic analysis of the FDC1 polymorphism that inhibits ferulic acid decarboxylation activity in industrial yeasts Reviewed

    Hatakeyama, A., Watanabe, Y., Arimoto, K., Hara, T., Joh, T.

    115 ( 4 )   249 - 254   2020.4

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    Other Link: http://id.ndl.go.jp/bib/030420156

  • The simple determination of urea in sake using a commercial assay kit Reviewed

    Nagai, Y., Suzuki, T., Hoshino, A., Kuribayashi, T., Hara, T., Joh, T.

    115 ( 2 )   109 - 114   2020.2

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    Other Link: http://id.ndl.go.jp/bib/030266241

  • 米アミロース含量と玄米に対する湿熱処理が高脂肪飼料投与ラットの糖質・脂質代謝に及ぼす影響—Effect of Amylose Content in Rice and Heat-Moisture Treatment of Brown Rice on Carbohydrate and Lipid Metabolism in Rats Fed a High Fat Diet Reviewed

    細川 陽子, 春日 景太, 榎 康明, 斉藤 憲司, 城 斗志夫, 奥村 寿子, 菅原 正義

    ルミナコイド研究 : 日本食物繊維学会会誌   23 ( 2 )   47 - 54   2019.12

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    Other Link: https://search.jamas.or.jp/link/ui/2020209331

  • Variations in 1-octen-3-ol and lipoxygenase gene expression in the oyster mushroom Pleurotus ostreatus according to fruiting body development, tissue specificity, maturity, and postharvest storage Reviewed

    Yuji Tasaki, Daiki Kobayashi, Ryoji Sato, Shunya Hayashi, Toshio Joh

    Mycoscience   60 ( 3 )   170 - 176   2019.5

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    Publishing type:Research paper (scientific journal)   Publisher:The Mycological Society of Japan  

    DOI: 10.1016/j.myc.2019.02.005

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  • Simple Discrimination of Sake(a Japanese Traditional Alcoholic Beverage)based on Trace Inorganic Ions using Colorimetric Assay Kits

    KURIBAYASHI Takashi, MIYAO Shunsuke, JOH Toshio, KANEOKE Mitsuoki

    JOURNAL OF THE BREWING SOCIETY OF JAPAN   114 ( 5 )   268 - 274   2019

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    DOI: 10.6013/jbrewsocjapan.114.268

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  • イヌワシAquila chrysaetos japonicaの主要餌動物における栄養成分と消化速度—Nutritive components and digestive rates of principal preys of the Golden Eagle Aquila chrysaetos japonica Reviewed

    布野 隆之, 関島 恒夫, 城 斗志夫, 阿部 學

    野生復帰 = Reintroduction / 野生復帰編集委員会 編   6 ( 1 )   13 - 18   2018.3

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  • 新潟の食の魅力を通して、大学生の食と健康への関心を高める教育プログラム Reviewed

    山口智子, 渡邊令子, 藤村 忍, 井上 誠, 城斗志夫, 谷口正之, 西海理之, 門脇基二

    新潟大学高等教育研究   5   29 - 34   2018.3

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    Language:Japanese   Publisher:新潟大学教育・学生支援機構  

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  • Breeding of a non-urea-producing sake yeast carrying a FAS2 mutation Reviewed

    Mycoscience : official journal of the Mycological Society of Japan   58 ( 4 )   302 - 306   2017.7

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  • Effects of super-hard rice bread blended with black rice bran on amyloid peptide production and abrupt increase in postprandial blood glucose levels in mice Reviewed

    Sumiko Nakamura, Takashi Hara, Toshio Joh, Atsushi Kobayashi, Akira Yamazaki, Kensaku Kasuga, Takeshi Ikeuchi, Ken'ichi Ohtsubo

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   81 ( 2 )   323 - 334   2017.2

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    DOI: 10.1080/09168451.2016.1240605

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  • Stable Isotope Analysis of Hydrogen and Oxygen in a Traditional Japanese Alcoholic Beverage, Sake, from Niigata Prefecture in Japan and Other Countries. Reviewed International journal

    Takashi Kuribayashi, Masamichi Sugawara, Keigo Sato, Yoshihito Nabekura, Toshio Aoki, Naoki Kano, Toshio Joh, Mitsuoki Kaneoke

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   33 ( 8 )   979 - 982   2017

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    Stable hydrogen and oxygen isotopic compositions (δD and δ18O values, respectively) were analyzed for "sake," a traditional Japanese alcohol beverage, to assess these values for the identification of its geographic origin. We collected sake (Junmai-shu; made of only rice, water, and koji) and its source water (i.e., brewing water) from breweries in Niigata Prefecture, Japan, and measured the isotopic compositions of water in these samples. The δD and δ18O values for the sake are well correlated with their respective values for the corresponding brewing water (δD; r = 0.92, δ18O; r = 0.80). Furthermore, based on the δD-δ18O cross plot, sake brewed in Niigata Prefecture is distinguishable from that brewed in countries other than Japan. These results imply that this dual isotope (δD and δ18O) analysis is potentially useful in identifying the geographic origin of sake.

    DOI: 10.2116/analsci.33.979

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  • A Saccharomyces cerevisiae strain encoding a novel FAS2 mutation produces high levels of caprylic acid Reviewed

    Yutaka Nagai, Toshiaki Suzuki, Susumu Yamashita, Toshio Joh, Yuji Tasaki

    MYCOSCIENCE   57 ( 3 )   228 - 231   2016.5

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    DOI: 10.1016/j.myc.2016.02.004

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  • Simple differentiation of sake (Japanese alcoholic beverage) based on trace inorganic components using colorimetric methods. Reviewed

    Takashi Kuribayashi, Keigo Sato, Toshio Joh, Mitsuoki Kaneoke, Ken-Ichi Watanabe

    Journal of Bioscience and Bioengineering   121 ( 1 )   43 - 46   2016.1

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    Several colorimetric methods were combined and used for the discrimination of commercial sake samples, based on their constituent inorganic components. The method was very rapid, simple, and did not require expensive equipment. Further, we showed that this method has potential application in immediate differentiation of sake by the visual inspection.

    DOI: 10.1016/j.jbiosc.2015.05.004

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  • Inhibitory Effect of gamma-Aminobutyric Acid (GABA) on Histamine Release from Rat Basophilic Leukemia RBL-2H3 Cells and Rat Peritoneal Exudate Cells Reviewed

    Azusa Kawasaki, Takashi Hara, Toshio Joh

    JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI   61 ( 8 )   362 - 366   2014

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    DOI: 10.3136/nskkk.61.362

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  • Mushroom flavor and the enzymes involved in the biosynthesis Invited

    JOH Toshio, KUDO Takunobu, TASAKI Yuji, FUJII Nisei, HARA Takashi

    Journal of Japan Association on Odor Environment   44 ( 5 )   315 - 322   2013.9

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    <p>The flavor is an important element constituting mushroom tastes. Therefore, the flavor compounds have been analyzed in various species of mushrooms. Though some mushrooms such as Matsutake and Shiitake have a characteristic flavor compound, major flavor compounds in most mushrooms are volatile C8 compounds: 1-octen-3-ol, 1-octen-3-one, 3-octanone and 3-octanol. Lipid peroxidation enzyme, hydroperoxide-cleaving enzyme and oxidoreductase are presumed to be involved in the synthesis, but little information has been obtained about the enzymes. In this paper, the flavor compounds in mushrooms and the enzymes involved in the biosynthesis are described.</p>

    DOI: 10.2171/jao.44.315

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  • Lactobacillus paracasei K71 Isolated from sakekasu (Sake Lees) Suppresses Serum IgE Levels in Ovalbumin-immunized Balb/c Mice Reviewed

    Takehisa Kumagai, Mariko Iguchi, Naoya Shigeyama, Sanae Okada, Toshio Joh, Takashi Hara

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   19 ( 1 )   127 - 132   2013.1

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  • Molecular Characterization of a Lipoxygenase from the Basidiomycete Mushroom Pleurotus ostreatus Reviewed

    Yuji Tasaki, Shungo Toyama, Takashi Kuribayashi, Toshio Joh

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 1 )   38 - 45   2013.1

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    DOI: 10.1271/bbb.120484

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  • Polyphenol Contents and Processing Properties of Screenings of Black-kerneled Glutinous Rice Cultivar ‘Shiho’ Reviewed

    Kobayashi Kazuyuki, Joh Toshio, Fukuyama Toshinori

    Japanese Journal of Crop Science   82 ( 2 )   192 - 196   2013

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    Black-kerneled rice contains antioxidants, making it of interest to consumers. However, it is much more expensive than common rice, and this is one of the problems for the food industry to utilize. To supply cheaper raw materials for the food industry, we assessed the polyphenol content and heat gelatinization properties of the screenings of ‘Shiho’ black-kerneled glutinous rice developed in Niigata Prefecture. Screenings of 1.70−1.85 mm grain thickness had 26.1% less polyphenol than refined grain, and screenings of \<1.70 mm had 54.3% less. Therefore, to achieve the same polyphenol yield as from refined grain, it would be necessary to use 30% to 50% more rice screenings. The rate of decrease of polyphenol content was lower per unit weight than per kernel, and it seems that flour made from screenings would reduce the necessary amount of raw materials in comparison with whole grains. Thinner grain had a higher protein content than thicker grain, and screenings of \<1.7 mm had 8.6% protein. In addition, the gelatinization start temperature was lower, and the rice cake hardening rate of rice screenings seemed to be lower than those of refined grain. The heat gelatinization properties of rice screenings also seemed to be inferior. Flour made from screenings would have a lower swelling capacity, a weaker gel strength, and slower starch retrogradation than that made from refined grain. It will be necessary to solve these problems before rice screenings can be used in the food industry. A supply route also needs to be established.

    DOI: 10.1626/jcs.82.192

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    Other Link: https://www.jstage.jst.go.jp/article/jcs/82/2/82_192/_pdf

  • High pressure can promote enzymatic hydrolysis of allergenic proteins in egg white to reduce IgE-binding activity.

    Watanabe, M, Hara, T, Nishiumi, T, Joh, T, Suzuki, A

    Proceedings of 7th International Conference on High Pressure Bioscience and Biotechnology   60   2012.10

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  • Mushroom flavor compounds and the biosynthesis mechanism. Invited

    Toshio Joh, Takunobu Kudo, Yuji Tasaki, Takashi Hara

    Aroma research : Journal of aroma science and technology   13 ( 1 )   26 - 30   2012.2

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  • Treatment of household food waste at high temperature and high pressure, and utilization of the resulting material as pig and chicken feeds. Reviewed

    Takateru Ishimori, Ayyakkannu Saravanan, Toshiyuki Sanada, Toshio Joh, Shinobu Fujimura, Tadayuki Nishiumi, Akio Imai, Tomoyuki Fujii, Hideyuki Sato, Hiroaki Kojima, Hidetaka Hori

    Journal of the Japan Society of Waste Management Experts   22 ( 4 )   253 - 266   2011.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society of Material Cycles and Waste Management  

    Food wastes from households were treated with steam at 150 °C for 40 minutes (150SFW) or at 200 °C for 150 minutes (200SFW), and the possibilities of developing the resulting materials into livestock feeds were researched. Both SFWs were shown to be composed about 20% of proteins and 20% of lipids, which are higher than the ratios of commercial diets for finishing pigs. On the other hand, pepsin digestibility of both SFWs was 26-56%, which was lower than that of a commercial diet. To estimate the applicability of SFWs as feed, eight pigs, each weighing approximately 70kg, were divided into test and control groups of four pigs each. In the test group, the pigs were fed diets that were 20% 200SFW for an initial 29 days and were subsequently fed diets that were 15% 150SFW until they reached 110±5kg. Pigs were fed diets without SFWs in the control group. The daily gains (DG) of two pigs in the test group were similar to those of pigs in the control group through the test period. The other two pigs in the test group had relatively lower DG when 200SFW was given, but ultimately reached 110±5kg 29-46 days later than pigs in the control group.

    DOI: 10.3985/jjsmcwm.22.253

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    Other Link: https://jlc.jst.go.jp/DN/JALC/00377522808?from=CiNii

  • Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko Reviewed

    Takunobu Kudo, Yuki Sato, Yuji Tasaki, Takashi Hara, Toshio Joh

    MYCOSCIENCE   52 ( 4 )   283 - 287   2011.7

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    DOI: 10.1007/s10267-010-0097-9

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  • Ability to Convert Soy Isoflavone Glucosides to Aglycones by Lactic Acid Bacteria from Various Plant Food Materials Reviewed

    Yaeko Ueno, Rie Mizutani, Takunobu Kudo, Takashi Hara, Toshio Joh

    JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI   58 ( 4 )   173 - 177   2011

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    DOI: 10.3136/nskkk.58.173

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  • Cultivation methods for improvement of yield, polyphenol content and characteristics for rice cake processing of black kerneled glutinous rice cultivar 'Shiho' in Niigata prefecture Reviewed

    Kazuyuki Kobayashi, Toshio Joh, Yoshihiko Takahashi, Toshinori Fukuyama

    Japanese Journal of Crop Science   79 ( 4 )   518 - 527   2010

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    DOI: 10.1626/jcs.79.518

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  • Peptides obtained by α-chymotryptic hydrolysis of obomucoid under high pressure reduce immunoreactivity and induce oral tolerance in mice. Reviewed

    Odani, S, Kanda, Y, Hara, T, Joh, T, Yamamoto, S, Nishiumi, T, Suzuki, A

    Review of High Pressure Science and Technology   19 ( S )   336   2009.7

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  • Effect of Cooking Water pH on Commercial Sterility in the Production of Cooked Rice Packed under Semi-aseptic Condition Reviewed

    Kinya Sakuma, Haruhiko Katoda, Tetsuya Fukaya, Toshio Joh, Akira Ito, Atsuo Watanabe

    Japan Journal of Food Engineering   9 ( 3 )   157 - 165   2008

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    DOI: 10.11301/jsfe2000.9.157

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  • A Study on Commercial Sterility of Cooked Rice Packed Under Semi-aseptic Condition from the Viewpoint of Cooking Condition Reviewed

    Kinya Sakuma, Haruhiko Katoda, Tetsuya Fukaya, Toshio Joh, Akira Ito, Atsuo Watanabe

    NIHON SHOKUHIN KOUGAKUKAISHI (Japan Journal of Food Engineering)   8 ( 4 )   295 - 304   2007

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    DOI: 10.11301/jsfe2000.8.295

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  • Effects of gluten-constituent protein compositions on chewing and swallowing characteristics of cookies. Reviewed

    10 ( 2 )   142 - 151   2006.8

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  • Structure and expression of two genes encoding secreted acid phosphatases under phosphate-deficient conditions in Pholiota nameko strain N2 Reviewed

    Y Tasaki, A Azwan, J Yazaki, T Hara, T Joh

    CURRENT GENETICS   49 ( 5 )   323 - 332   2006.5

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    DOI: 10.1007/s00294-006-0058-1

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  • Operating Conditions of Ultrafiltration (UF) for Clarification of Rice Washing Drainage from Packed Rice Cake Manufacturing Plant Reviewed

    Aila Rahaman, Akira Ito, Toshio JoH, Atsuo Watanabe, Tatsuma Kataoka, Masato Oonishi

    Japan Journal of Food Engineering   7 ( 3 )   189 - 195   2006

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    DOI: 10.11301/jsfe2000.7.189

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  • 包装餅製造工場洗米等排水の再生・リサイクルに適した限外濾過膜の選定 Reviewed

    Rahaman Aila, 城 斗志夫, 伊東 章

    日本食品工学会誌   6 ( 2 )   57 - 66   2005.6

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  • A Correlation Study of Concentration between Airborne Microorganisms and Airborne Particles Reviewed

    Masami Matsunaga, Chiryo Tsunoda, Toshio Jo, Akira Ito, Atsuo Watanabe

    NIHON SHOKUHIN KOUGAKUKAISHI (Japan Journal of Food Engineering)   6 ( 3 )   197 - 204   2005

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    DOI: 10.11301/jsfe2000.6.197

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  • Selection of Ultrafiltration (UF) Membrane Modules for Clarification of Rice Washing Drainage from Packed Rice Cake Manufacturing Plant Reviewed

    Aila Rahaman, Toshio Jyou, Akira Itou, Atsuo Watanabe, Tatsura Kataoka, Masato Oonishi

    Japan Journal of Food Engineering   6 ( 2 )   151 - 160   2005

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    DOI: 10.11301/jsfe2000.6.151

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  • Basic Study for High Temperature and Short Time Sterilization by Continuous Microwave Heating for Solid Foods or High Viscous Foods Reviewed

    Tetsuya Takatomi, Akira Ito, Toshio Joh, Atsuo Watanabe

    NIHON SHOKUHIN KOUGAKUKAISHI (Japan Journal of Food Engineering)   6 ( 3 )   189 - 196   2005

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    DOI: 10.11301/jsfe2000.6.189

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  • Effect of high pressure treatment on the allergenicity of bovine serum albumin evaluated by histamine release assay using sera from allergic patients and human basophilic KU812F cells. Reviewed

    Suzuki, A, Hara, T, Koyama, H, Nogami, N, Han, G.D, Odani, S, Matsuno, M, Joh, T, Nishiumi, T

    Proc. 50th Int. Cong. Meat Sci. Technol.   CD-ROM   1315 - 1318   2004.8

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  • Three genes specifically expressed during phosphate deficiency in Pholiota nameko strain N2 encode hydrophobins Reviewed

    Y Tasaki, K Ohata, T Hara, T Joh

    CURRENT GENETICS   45 ( 1 )   19 - 27   2004.2

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    DOI: 10.1007/s00294-003-0457-5

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  • Structure and expression of a phosphate deficiency-inducible ribonuclease gene in Pholiota nameko Reviewed

    Y Tasaki, A Azwan, T Hara, T Joh

    CURRENT GENETICS   45 ( 1 )   28 - 36   2004.2

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    DOI: 10.1007/s00294-003-0463-7

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  • Development of new type clarified apple juice containing partially enzymatic degraded pectin through ceramic MF membrane Reviewed

    S Harada, R Suzuki, Y Tanii, T Joh, H Ohta, A Watanabe

    JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI   51 ( 3 )   123 - 130   2004

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    DOI: 10.3136/nskkk.51.123

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  • Development of New Type Optical Particle Counter System to Control Biological Clean Room for Aseptic Filling and Packaging Reviewed

    Masami Matsunaga, Chiryo Tsunoda, Toshio Jo, Akira Ito, Atsuo Watanabe

    Japan Journal of Food Engineering   5 ( 2 )   121 - 128   2004

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    DOI: 10.11301/jsfe2000.5.121

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  • Basic Study for Ultra High Temperature Short Time Sterilization with Microwave for Solid Foods and Viscous Foods Reviewed

    Tetsuya Takatomi, Akira Ito, Toshio Joh, Atsuo Watanabe

    NIHON SHOKUHIN KOUGAKUKAISHI (Japan Journal of Food Engineering)   5 ( 4 )   235 - 241   2004

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    DOI: 10.11301/jsfe2000.5.235

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  • Triacylglycerol lipase participates in the formation of n-hexanal in alfalfa seedlings Reviewed

    Chiharu Uno, Takashi Hara, Toshio Joh

    Food Science and Technology Research   9 ( 2 )   185 - 189   2003

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    DOI: 10.3136/fstr.9.185

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  • Purification and characterization of lipoxygenase from Pleurotus ostreatus Reviewed

    T Kuribayashi, H Kaise, C Uno, T Hara, T Hayakawa, T Joh

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   50 ( 5 )   1247 - 1253   2002.2

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    DOI: 10.1021/jf0112217

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  • Gene expression during Pi deficiency in pholiota nameko: Accumulation of mRNAs for two transporters Reviewed

    Yuji Tasaki, Yuki Kamiya, A. Azwan, Takashi Hara, Toshio Joh

    Bioscience, Biotechnology and Biochemistry   66 ( 4 )   790 - 800   2002

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    DOI: 10.1271/bbb.66.790

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  • Formation Mechanism of Grassy Odor Substance in Alfalfa Seedlings Reviewed

    Chiharu Uno, Takashi Hara, Toshio Joh

    Food Science and Technology Research   8 ( 4 )   347 - 352   2002

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    DOI: 10.3136/fstr.8.347

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  • Effect of phosphate deficiency on growth and protein profile in three strains of Pholiota nameko Reviewed

    Yuji Tasaki, A. Azwan, Takashi Hara, Toshio Joh

    Mycoscience   42 ( 6 )   489 - 498   2001.12

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    DOI: 10.1007/BF02460947

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  • Induction and secretion of RNA-degrading enzymes by phosphate deficiency in Pholiota nameko Reviewed

    Toshio Joh, Yuji Tasaki, Takashi Hara, Toshiro Hayakawa

    Bioscience, Biotechnology and Biochemistry   65 ( 11 )   2561 - 2564   2001.11

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    DOI: 10.1271/bbb.65.2561

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  • リン酸欠乏に伴うナメコ菌糸体および培養ろ液中のリンの量的変化

    田﨑裕二, 城斗志夫, 小熊千佳子, 土田努, 早川利郎

    新潟大農学部研究報告   52 ( 2 )   93 - 99   2000.3

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  • The pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases from wheat bran of triticum aestivum L. cv. nourin #61 Reviewed

    Tadao Nakano, Toshio Joh, Kazumasa Narita, Toshiro Hayakawa

    Bioscience, Biotechnology and Biochemistry   64 ( 5 )   995 - 1003   2000.1

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    DOI: 10.1271/bbb.64.995

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  • Cryoprotective activities of group 3 late embryogenesis abundant proteins from chlorella vulgaris C-27 Reviewed

    Ken-ichi Honjoh, Hiroko Matsumoto, Hideyuki Shimizu, Kanae Ooyama, Kageyuki Tanaka, Yuichi Oda, Ryoji Takata, Toshio Joh, Koushirou Suga, Takahisa Miyamoto, Masayoshi Iio, Shoji Hatano

    Bioscience, Biotechnology and Biochemistry   64 ( 8 )   1656 - 1663   2000

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    DOI: 10.1271/bbb.64.1656

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  • Characterization of the Manufacturing Process of Rice Crackers by Differential Scanning Calorimetry Reviewed

    WATANABE Toshiyuki, JOH Toshio, HAYAKAWA Toshiro

    NIPPON SHOKUHIN KOGYO GAKKAISHI   46 ( 2 )   51 - 58   1999.2

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    Production process of rice crackers (arare, okaki, senbei) is time consuming and complicating. Therefore, it is difficult to explain the process scientifically. For the explanation of the manufacturing process and the changes of the gelatinized and retrograded rice starch gel produced in its process, we analyzed the products in the manufacturing process and the imitative manufacturing process of glutinous rice crackers by differential scanning calorimetry. As the result, it was proved that the steaming process was to break hydrogen bonds by the gelatinization phenomenon of starch, the refrigeration process was to create the reformation structure by the retrogradation phenomenon of starch; to create new hydrogen bonds, drying process was to create more thermostable reformation structure by the annealing phenomenon of starch and baking process was to break its reformation structure. Furthermore, it was proved that all process was related to each other and affect the glutinous rice crackers (final products). Namely, it was proved that DSC could analyze the manufacturing process of glutinous rice crackers and the changes of the gelatinized and retrograded rice starch gel produced in its process.

    DOI: 10.3136/nskkk.46.51

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  • Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61 Reviewed

    Tadao Nakano, Toshio Joh, Emi Tokumoto, Toshiro Hayakawa

    Food Science and Technology Research   5 ( 1 )   18 - 23   1999

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    DOI: 10.3136/fstr.5.18

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  • Effect of starch properties of the extent of breakage non-glutinous dried rice cake Reviewed

    Youichi Yoshii, Masami Arisaka, Toshio Joh, Toshiro Hayakawa

    Food Sci. Technol. Int. Tokyo   4 ( 2 )   121 - 124   1998.12

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    This study was conducted to determine the reason why dried rice cakes for non-glutinous rice crackers (<I>Senbei</I>) often break in the production process. Breakage after drying at 40°C for 12 h was found to be due to milling and storage conditions of the rice flour. Gel permeation chromatography revealed that the molecular weight of starch decreased as the extent of breakage increased. Damage to rice flour starch differed according to the milling methods. When press roller milled rice flour was stored at 5-25°C, the activity of α-amylase increased as the temperature became higher. The total water-soluble carbohydrate content of dried rice cake may be a good criterion to judge the extent of breakage. Reduction of the molecular sizes of starch, due to milling and action of α-amylase, is considered to cause the breakage of dried rice cake.

    DOI: 10.3136/fsti9596t9798.4.121

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  • Purification and characterization of glucose-1-phosphatase from mycelia of Pholiota nameko Reviewed

    Junshi Yazaki, Toshio Joh, Kayo Suzuki, Kaya Ogawa, Toshiro Hayakawa

    Mushroom Science and Biotechnology   6 ( 1 )   1 - 8   1998.12

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  • Electrophoretic analysis of soluble proteins specifically synthesized under phosphate deficiency in the mycelia of Pholiota nameko Reviewed

    Toshio Joh

    Mycoscience   39 ( 2 )   195 - 198   1998.7

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    DOI: 10.1007/BF02464059

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  • Isolation and properties of glucose-1-phosphatase from mycelia of pholiota nameko Reviewed

    Toshio Joh, Junshi Yazaki, Kayo Suzuki, Toshiro Hayakawa

    Bioscience, Biotechnology and Biochemistry   62 ( 11 )   2251 - 2253   1998

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    DOI: 10.1271/bbb.62.2251

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  • Physicochemical properties of low-amylose rice Reviewed

    Youichi Yoshii, Masami Arisaka, Toshio Jou, Toshirou Hayakawa

    Nippon Shokuhin Kagaku Kogaku Kaishi   44 ( 5 )   353 - 360   1997

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    DOI: 10.3136/nskkk.44.353

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  • Acid phosphatase isozymes secreted under phosphate-deficient conditions in Pholiota nameko Reviewed

    Junshi Yazaki

    Mycoscience   38 ( 3 )   347 - 350   1997

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    DOI: 10.1007/BF02464095

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  • ナメコ菌糸体および培養ろ液のリン酸欠乏に伴う可溶性蛋白質の変化 Reviewed

    城斗志夫, 田﨑裕二, 矢崎潤史, 早川利郎

    日本菌学会会報   37 ( 1 )   147 - 154   1996.12

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  • Identification of myo-inositol monophosphates in mycelia of Pholiota nameko Reviewed

    Toshio Joh, Dia Habib Malick, Yuji Tasaki, Toshiro Hayakawa

    Mycoscience   37 ( 11 )   381 - 383   1996.11

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    DOI: 10.1007/BF02461313

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  • Modified preparation method and identification of inositol monophosphate

    Habib Malick Dia, Junko Takahashi, Toshio Joh, Toshiro Hayakawa

    Bull. Facul;Agric. Niigata Univ.   48   93 - 98   1996.3

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  • Purification and characterization of secreted acid phosphatase under phosphate-deficient condition in Pholiota nameko Reviewed

    Toshio Joh, Dia Habib Malick, Junshi Yazaki, Toshiro Hayakawa

    Mycoscience   37 ( 1 )   65 - 70   1996

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    DOI: 10.1007/BF02461459

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  • Isolation and characterization of hardening-induced proteins in chlorella vulgaris c-27: Identification of late embryogenesis abundant proteins Reviewed

    Ken-Ichi Honjoh, Makoto Yoshimoto, Toshio Joh, Taishin Kajiwara, Takahisa Miyamoto, Shoji Hatano

    Plant and Cell Physiology   36 ( 8 )   1421 - 1430   1995.12

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  • Molecular cloning and expression of hardening-induced genes in chlorella vulgaris C-27: The most abundant clone encodes a late embryogenesis abundant protein Reviewed

    Toshio Joh, Ken-Ichi Honjoh, Makoto Yoshimoto, Jun Funabashi, Takahisa Miyamoto, Shoji Hatano

    Plant and Cell Physiology   36 ( 1 )   85 - 93   1995.1

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  • グルタルアルデヒド架橋米糠酸性ホスファターゼの物理化学的諸性質に関する研究

    佐藤政幸, 金内喜昭, 大桃哲郎, 鐘国安, 城斗志夫, 早川利郎

    新潟大学農学部研究報告   46   79 - 90   1994.3

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  • Changes in Translatable RNA Population during Hardening of Chlorella ellipsoidea C-27

    Joh, T, Yoshimoto, M, Honjoh, K, Miyamoto, T, Hatano, S

    J. Fac. Agr., Kyushu Univ.   37 ( 3・4 )   257 - 263   1993.3

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  • Composition and positional distribution of fatty acids in polar lipids from Chlorella ellipsoidea differing in chilling susceptibility and frost hardiness Reviewed

    Toshio Joh, Tsuguhiko Yoshida, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano

    Physiologia Plantarum   89 ( 2 )   285 - 290   1993

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    DOI: 10.1111/j.1399-3054.1993.tb00155.x

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  • Preparation of Protoplasts from Chlorella ellipsoidea C-27. Reviewed

    Hatano, S, Joh, T, Miyamoto, M, Yoshimoto, M

    Plant Cell Physiol.   33 ( 5 )   651 - 655   1992.7

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  • Changes in Soluble and Membrane Proteins of Chlorella ellipsoidea during Early Time of Hardening

    YOSHIMOTO M., Okamura Hideki, Joh Toshio, Miyamoto Takahisa, Hatano Shoji

    J. Fac. Arg., Kyushu Univ.   36 ( 1 )   69 - 77   1991.10

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Books

  • 21世紀は『食べる』時代/『食べる』を考えたことありますか?

    ( Role: Joint author ,  日本人と新潟米)

    医歯薬出版株式会社  2016.8 

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  • 菌類の事典

    日本菌学会( Role: Joint author ,  香料)

    朝倉書店  2013.10  ( ISBN:9784254171471

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  • Traditional and modern Japanese soy foods : manufacturing, nutrition and cuisine of a variety of soy foods for health and joy of taste

    大山, 卓爾, 高橋, 能彦, 城, 斗志夫, Whitaker, Andrew Charles, 西脇, 俊和, 諸橋, 敬子, 渡辺, 聡, 下條, 明( Role: Joint author ,  Chapter4 Tonyu(Soymilk))

    Nova Science Publishers  2013.10  ( ISBN:9781626180017

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  • 高圧力下の生物科学「超高圧によるウシ血清アルブミンのアレルゲン性低減化と構造変化との関連」

    野上直行, 松野正知, 原崇, 城斗志夫, 西海理之, 鈴木敦士( Role: Joint author ,  95-101)

    さんえい出版  2006.11 

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  • 新潟発『食べる』

    城斗志夫( Role: Joint author ,  54-59)

    新潟日報事業社  2005.5  ( ISBN:4861321166

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MISC

  • 科学教育の重要性—物事を科学的に判断できる人間を育てるために—

    城斗志夫

    創造   2011.8

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  • Elimination of the allergenicity of food protein by high pressure

    Naoyuki Nogami, Toshio Joh, Masatomo Matsuno, Tadayuki Nishiumi, Takashi Hara, Atsushi Suzuki

    Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu   16 ( 1 )   11 - 16   2006

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    Language:Japanese   Publishing type:Book review, literature introduction, etc.  

    DOI: 10.4131/jshpreview.16.11

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  • 偉大なり!キノコパワー(第1回)

    城斗志夫

    月刊新潟バイタリティー   23 ( 1 )   33 - 35   2004.7

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  • 偉大なり!キノコパワー(第1回)

    城斗志夫

    月刊新潟バイタリティー   22 ( 12 )   52 - 53   2004.6

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  • 偉大なり!キノコパワー(第1回)

    城斗志夫

    月刊新潟バイタリティー   22 ( 11 )   38 - 39   2004.5

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  • Purification and Characterization of Phytase Isozymes from Wheat Bran

    NAKANO Tadao, JOH Toshio, HAYAKAWA Toshiro

    Bulletin of the Faculty of Agriculture, Niigata University   49 ( 2 )   119 - 128   1997.3

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  • ナメコ菌糸体における構成型酸性ホスファターゼ : 微生物

    矢崎 潤史, 富田 真一, 杉田 輝, 城斗 志夫, 早川 利郎

    日本農藝化學會誌   70   122 - 122   1996.3

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  • リン欠乏により誘導されるナメコ菌糸体の酸性ホスファターゼ : アイソザイムのペプチド部の構造と糖鎖の機能 : 微生物

    矢崎 潤史, 城 斗志夫, 早川 利郎

    日本農藝化學會誌   69   83 - 83   1995.7

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  • リン欠乏により誘導されるナメコ菌糸体の酸性ホスファターゼ : アイソザイムのサブユニット構造 : 微生物

    城 斗志夫, 矢崎 潤史, 石動 政直, 早川 利郎

    日本農藝化學會誌   69   83 - 83   1995.7

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  • Structure and Expression of Hardening-Induced Genes in Chlorellaellipsoidea

    Toshio Joh

    Memoirs of the Faculty of Agriculture, Niigata University   31   1 - 80   1994.3

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Presentations

  • ヒラタケにおける香気成分3-オクタノンの生合成に関与するエノンレダクターゼ遺伝子の解析

    工藤卓伸, 松山遥平, 原崇, 城斗志夫

    日本農芸化学会  2011.3 

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  • Gene cloning of a Pleurotus ostreatus lipoxygenase involved in the biosynthesis of mushroom aroma International conference

    Yuji TASAKI, Shungo TOYAMA, Toshio JOH

    The 6th Meeting of East Asia for Mushroom Science  2010.11 

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  • 血中高IgEレベルマウス脾細胞のIFN-γおよびIL-4産生におよぼすγ-アミノ酪酸(GABA)の影響

    石川公亮, 寺平拓也, 和田紘稔, 原崇, 城斗志夫

    日本食品科学工学会  2010.9 

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  • Lactobacillus paracasei K17の経口投与がBALB/cマウスの抗体産生並びに細胞傷害活性に及ぼす影響

    五十嵐晶, 寺平拓也, 熊谷武久, 斉藤真理子, 渡辺紀之, 城斗志夫, 原崇

    日本食品科学工学会  2010.9 

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  • 露地栽培したヒラタケの香気成分と香気生合成関連酵素の発現解析

    工藤卓伸, 松山遥平, 原崇, 城斗志夫

    日本きのこ学会  2010.9 

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  • ヒラタケの揮発性C8化合物生合成に及ぼすリポキシゲナーゼとエノンレダクターゼの影響

    工藤卓伸, 清水亮祐, 原崇, 城斗志夫

    日本農芸化学会  2010.3 

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  • Lactobacillus paracasei K17摂取によるTh1並びにTh2サイトカイン産生応答の抑制

    石川公亮, 熊谷武久, 斉藤真理子, 重山直也, 山崎春佳, 城斗志夫, 渡辺紀之, 原崇

    日本農芸化学会  2010.3 

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  • 大豆イソフラボンに対し高いアグリコン変換能を持つ植物由来乳酸菌のβ-グルコシダーゼ

    上野八重子, 李新剛, 工藤卓伸, 原崇, 城斗志夫

    日本農芸化学会  2010.3 

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  • 植物由来乳酸菌の大豆イソフラボンアグリコン変換能とβ-グルコシダーゼ活性の気質特異性

    上野八重子, 水谷理絵, 李新剛, 工藤卓伸, 原崇, 城斗志夫

    日本食品科学工学会  2009.9 

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  • 酒粕由来乳酸菌Lactobacillus paracasei K71のIgE産生抑制作用

    重山直也, 斉藤真理子, 熊谷武久, 渡辺紀之, 岡田早苗, 城斗志夫, 原崇

    日本食品科学工学会  2009.9 

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  • エンドウスプラウトのアンジオテンシンI変換酵素阻害活性

    中澤大樹, 村山里子, 原崇, 城斗志夫

    日本食品科学工学会  2009.9 

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  • オバルブミン感作したBALB/cマウスにおけるIgE並びにサイトカインの産生に及ぼすイノシトール6リン酸の影響

    石川公亮, 原崇, 城斗志夫

    日本食品科学工学会  2009.9 

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  • 白色系統マイタケ熱水抽出物のIgE産生抑制作用

    堀あゆみ, 原崇, 城斗志夫

    日本食品科学工学会  2009.9 

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  • 高圧処理がキノコ熱水抽出物の抗アレルギー活性におよぼす影響

    野澤祥史, 五十嵐晶, 佐藤可奈, 原崇, 城斗志夫

    生物関連高圧研究会  2009.7 

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  • 米発酵食品由来乳酸菌のIgE産生抑制作用

    原崇, 熊谷武久, 斉藤真理子, 重山直也, 城斗志夫, 渡辺紀之, 岡田早苗

    日本農芸化学会  2009.3 

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  • NC/Ngaマウスのアトピー性皮膚炎様症状に対する植物性乳酸菌Lactobacillus paracasei K71の改善効果

    斉藤真理子, 熊谷武久, 渡辺紀之, 重山直也, 城斗志夫, 原崇

    日本農芸化学会  2009.3 

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  • ナメコ菌糸体のリン酸欠乏適応機構

    工藤卓伸, 田崎裕二, 原崇, 城斗志夫

    日本農芸化学会  2009.3 

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  • ヒラタケのリポキシゲナーゼのcDNAクローニング

    田崎裕二, 笠原啓介, 外山峻吾, 栗林喬, 原崇, 城斗志夫

    日本農芸化学会  2009.3 

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  • 発光ダイオードの照射がトマトスプラウトの色素成分に及ぼす影響

    清塚桂輔, 牛田悠介, 松本年弘, 稲熊隆博, 原崇, 城斗志夫

    日本農芸化学会  2009.3 

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  • Quantification and Structure Analysis of Arabinoxylan in Rice Bran of Various Cultivars International conference

    Masahiro Kobayashi, Akira Goto, Takashi Hara, Toshio Joh

    Second International Symposium on Rice and Disease Prevention  2008.10 

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  • Inductive Effect of Inositol Hexaphosphate (IP6) on Production of β-defensin2 and β-defensin3 in Human Lung Epithelial A549 Cells International conference

    Yousuke Shinohara, Takashi Hara, Toshio Joh

    Second International Symposium on Rice and Disease Prevention  2008.10 

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  • ゴボウスプラウトに含まれる抗酸化物質

    村山里子, 沓澤真子, 原崇, 城斗志夫

    日本農芸化学会  2008.3 

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  • γ-アミノ酪酸のヒト好塩基球様KU812F細胞に対するヒスタミン遊離抑制効果

    本間香織, 原崇, 松野正知, 城斗志夫

    日本農芸化学会  2008.3 

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  • イノシトール6リン酸(InsP6)のヒト肺上皮細胞株A549に対するβ-デフェンシン2の産生誘導

    篠原陽介, 原崇, 城斗志夫

    日本農芸化学会  2008.3 

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  • ナメコ菌糸体がリン酸欠乏下で特異的に発現するハイドロフォビンの特性

    工藤卓伸, 佐藤有希, 原崇, 城斗志夫

    日本農芸化学会  2008.3 

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  • タラの芽葉茶の抗アレルギー作用に関する研究

    本間香織, 原崇, 大橋義治, 城斗志夫

    日本農芸化学会  2007.3 

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  • 超高圧下におけるオボムコイドのキモトリプシン消化促進とアレルゲン性低減化

    神田泰子, 鈴木敦士, 松野正知, 原崇, 城斗志夫, 小谷スミ子

    日本農芸化学会  2007.3 

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  • ヒト好中球様HL-60細胞のオプソニン化ザイモサン貪食に及ぼす種々のイノシトールリン酸の影響

    岸大輔, 原崇, 城斗志夫

    日本農芸化学会  2007.3 

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  • Biosynthesis pathway of 1-octen-3-ol in Fleurotus ostreatus International conference

    城斗志夫, 原崇

    Proceedings of The International Symposium of Mushroom Science  2006.9 

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  • 超高圧と酵素の併用により分解処理したオボムコイドの加水分解性と抗体結合性

    神田泰子, 鈴木敦士, 松野正知, 原崇, 城斗志夫, 小谷スミ子

    日本食品科学工学会  2006.8 

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  • タラの芽葉茶の含有成分と機能性に関する研究

    本間香織, 原崇, 城斗志夫

    日本食品科学工学会  2006.8 

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  • イノシトール6リン酸によるヒト好中球様HL-60細胞の貪食能増強

    岸大輔, 原崇, 篠原陽介, 城斗志夫

    日本食品科学工学会  2006.8 

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Awards

  • 日本缶詰協会技術賞

    2004.11   日本缶詰協会  

    原田三郎, 鈴木涼子, 谷猪由起, 渡辺敦夫, 城斗志夫, 太田英明

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Research Projects

  • 細胞壁からフェルラ酸を遊離する麹菌由来フェルラ酸エステラーゼの解析

    Grant number:24K08744

    2024.4 - 2027.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    城 斗志夫

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

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  • 植物由来乳酸菌が産生するイソフラボン配糖体特異的糖質分解酵素の解析

    Grant number:21K05437

    2021.4 - 2024.3

    System name:科学研究費助成事業

    Research category:基盤研究(C)

    Awarding organization:日本学術振興会

    城 斗志夫

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    大豆イソフラボンの大部分は吸収されにくい配糖体として存在し、吸収率の向上には腸内細菌が産生するβ-グルコシダーゼによる糖の分解が不可欠である。本研究は、申請者が植物性食材から単離したイソフラボン配糖体分解能が高い乳酸菌株Leuconostoc mesenteroides KM3を用い、この菌が産生するβ-グルコシダーゼの解析を通して配糖体分解能の高さの要因を解明するとともに、より配糖体分解能の高い酵素の作出を試みるものである。他の菌にはないイソフラボン配糖体に高い特異性を持つβ-グルコシダーゼの存在が配糖体分解能の高さの要因と推定しており、令和3年度は配糖体特異的β-グルコシダーゼの存在を明らかにすることを目的に研究を行った。β-グルコシダーゼの活性測定には合成基質であるp-ニトロフェニル-β-グルコピラノシド(β-pNPG)を通常用いるが、特異的酵素の検出のために配糖体の一種であるダイジンでも活性を測定した。培養したKM3の菌体懸濁液を酵素液として両基質で活性を測定した結果、β-pNPGに対しても活性を示したが、ダイジンに対する活性の方が非常に高かった。比較対象としてイソフラボン配糖体分解能が低い乳酸菌で同様に活性を測定した結果、β-pNPGに対する活性とダイジンに対する活性は同程度であった。次にKM3の菌体を超音波破砕し、遠心分離により細胞壁画分と細胞内画分に分離し、両画分を酵素液として2つの基質で酵素活性を測定した。その結果、β-pNPGに対する活性は細胞壁画分で高く、ダイジンに対する活性は細胞内画分で高かった。この結果は、KM3が配糖体特異的酵素と配糖体非特異的酵素の少なくとも2種類のβ-グルコシダーゼを産生し、その局在性が異なることを示している。また、配糖体特異的酵素は細胞内画分を用いることでさらに精製できるという次につながる重要な知見が得られた。

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  • Production of gluten free rice flour bread to which nutrients and functional ingredients are added

    Grant number:24500952

    2012.4 - 2015.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    ODANI Sumiko, JOH Toshio, YAMAGUCHI Tomoko

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    Grant amount:\5070000 ( Direct Cost: \3900000 、 Indirect Cost:\1170000 )

    Bread-making quality of the brown rice powder produced by the roll milling method was inferior to that of the white rice flour produced by the enzyme processing-milling method. This was because the average grain diameter, the starchy damage level and the water absorption coefficient of the brown rice powder were higher than those of the white rice flour. The amounts of γ-aminobutylic acid (GABA) which is one of the functional ingredients of brown rice were increased at 3.4 times after the first fermentation process. But that decreased at 2.2 times after the secondary fermentation and the baking process. It is suggested that the decrease of GABA was caused by the yeast work and the browning reaction (amino-carbonyl reaction) by baking bread. It will possible to produce the GABA rich brown rice bread by improving the bread-making conditions such as the dough's pH, the fermentation temperature and the baking time.

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  • Development of food products based on gluten for the nursing of individuals with eating and swallowing disorders

    Grant number:14370629

    2002 - 2004

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    NOMURA Akiko, NOMURA Shuichi, YAMADA Yoshiaki, KOHNO Shoji, TAKAHASHI Hajime, EGAWA Hiroko

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    Grant amount:\6100000 ( Direct Cost: \6100000 )

    The purpose of this study was to search for foods that are appropriate for dependent people with eating and swallowing disorders. To achieve this purpose, we investigated the following properties of foods : toughness, adherence, and aggregation.
    In the academic year 2002, we focused on gluten, which has high protein content, but is low in calories. We tried to separate from commercially available flour the two constituent proteins of gluten, gliadin and glutenin. However, we did not succeed in separating high-purity gliadin and glutenin.
    In the academic year 2003, we obtained from a food company glutenin and gliadin that were not of high purity and use them to produce various types of cookies by changing the blending ratio of these proteins. Consequently, we investigated the properties of these cookies and found out the blending ratio of glutenin and gliadin that was meeting the conditions for food recommended to dependent people with swallowing disorders.
    In the academic year 2004, referring to the results of the previous year, we increased the number of sample food types that we adjusted, measured their properties and then we confirmed their ease of mastication in a clinical trial. Moreover, we showed that oral functions and general condition of the dependent people are greatly influenced by the form of their meals.
    The results of this research were presented in part at the 15^<th> Annual Meeting of the Japanese Society of Gerodontology, the 10^<th> Annual Meeting of the Japanese Society of Dysphagia Rehabilitation, and the 3^<rd> Annual Meeting of the Scientific Society of the Meirin College.
    As a future project, we intend to develop instruments for oral care that can help in the rehabilitation of the eating and swallowing functions of people with a low degree of dependence.

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  • 食用キノコの香気分子生合成機構の解明と香気の安定生成に関する基礎的研究

    Grant number:11760101

    1999 - 2000

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    城 斗志夫

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    Grant amount:\2300000 ( Direct Cost: \2300000 )

    食用キノコの主要香気成分、1-オクテン-3-オールの生合成機構の解明を目的として、その生合成に深く関与すると考えられるリポキシゲナーゼ(LOX)を香り高いことで知られているヒラタケ(Pleurotus ostreatus)から単一に精製し、合成機構との関連を検討した。
    ヒラタケの傘をブレンダーと超音波破砕機でホモジナイズ後、遠心して粗酵素液を調製した。これをセファクリルS-400ゲルろ過カラム、ダイマトレックスグリーンAアフィニティーカラム、DEAE-トヨパールイオン交換カラムの3つのステップで精製した。その結果、LOXは126倍に精製され、回収率は5%、比活性33U/mgの蛋白質が得られた。精製酵素をSDS-PAGEで分析したところ一本のバンドしか検出されず、上記の方法でLOXは均一に精製されたことがわかった。精製酵素のゲルろ過による分子量は72,000で、SDS-PAGEでの分子量が67,000であったことから同酵素は単一のサブユニットから構成されていると考えられた。酵素反応の最適条件は25℃、pH8.0であり、本酵素は40℃以下、pH5〜9で安定だった。また、原子吸光分析と吸光スペクトル分析により本酵素は非ヘム型のFe原子を持つことがわかった。精製酵素は脂肪酸のうちリノール酸に高い特異性を示し、その反応生成物を調べた結果、13-ヒドロペルオキシドを特異的に生成していた。キノコの1-オクテン-3-オール生合成経路には9-ヒドロペルオキシドを経た経路と13-ヒドロペルオキシドを経た経路の2つの説があり、ヒラタケの結果は後者により1-オクテン-3-オールが合成されることを示唆している。さらに、露地栽培されたヒラタケのLOX活性を収穫時期である秋から冬にかけ測定したところ、収穫初期の10月頃で最も高く、寒くなるにつれ低下することがわかり、人が感じる香りの強度変化と一致していた。

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  • Studies on structure and generation of volatile formating gene in edible mushrooms

    Grant number:07456154

    1995 - 1997

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    HAYAKAWA Toshiro, JOH Toshio, MITSUI Toshiaki

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    Grant amount:\1100000 ( Direct Cost: \1100000 )

    Induction of lipoxygenase in mycelia of Shiitake mushroom (Lentinus edodes) was studied.
    Fresh weight of mycelia cultured for 3 weaks in soybean medium, in which soybean oil and flour were respectively used as carbon and nitrogen sources, was 11-fold that in GC medium, in which goucose and casamino acids were respectively used as carbon and nitrogen sources. Mycelial forms of both cultures differed greatly : GCculture, a large spherical form ; soybean soybean culture, a small spherical form or a fibriform. Lipoxygenase activity of mycelia cultured in soybean medium was 26-fold higher than the corresponding activity in GC medium, indicating the induction of lipoxygenase in mycelia by soybean culture. Optimum concentrations of soybean oil and flour in the medium for the growth of mycelia and the induction of lipoxygenase were 60 ml/1 and 12g/1, respectively. Active staining of lipoxygenase revealed that among 4 lipoxygenase isozymes in mycelia of GC culture only one isozyme is induced by soybean culture. The induced lipoxygenase showed high activity below pH 4.5, and was stable at pH 3.0 to 8.5 and below 50゚C.The enzyme had a higher specificity against linoleic acid and arachidonic acid than against linolenic acid. Cu^<2+>, N-ethylmaleimide and indoactic acid inhibited the enzyme activity.
    Analysis of Volatile compouds from Maitake mushroom (Grifola frondosa) homogenate was done by gaschromatograph equipped with PEG 20M fused silica capilary column (i.d.0.25 mm x 60 m).
    The capacity of maitgake lipoxygenase and hydroperoxidelyase to generate short-chaine volatile carbonyl compounds depended on the concentrations of enzyme, substrate fatty acids, and duration of incubation period. The major volatile compounds generated from the oxidation of linoleic acid by lipoxygenase and hydroperoxide lyase in maitake homogenate were 1-octen-3-ol (60%), 3-octenol (31%), 2-octenal (3%), n-heptanal (2.2%), methyl cinnamate (1.5%), nerolidol (1.3%), 2-dcanone (0.8%), n-octanal (0.4%), 3-octen-3one, and etc.
    The molecular weight of lipoxigenase from Hiratake mushroom (Pleurotuso streatus), was 87.00 by the use of gel chromatography with Sephacryl S-400.

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Teaching Experience

  • 地域交流サテライト実習

    2025
    Institution name:新潟大学

  • 1・2年生対象 長期・企業実践型プログラムI

    2024
    Institution name:新潟大学

  • 1・2年生対象 長期・企業実践型プログラムII

    2024
    Institution name:新潟大学

  • 有機化学実験(農)

    2023
    Institution name:新潟大学

  • 食の安全・安心論

    2022
    Institution name:新潟大学

  • 食品科学演習II

    2022
    -
    2023
    Institution name:新潟大学

  • 統合化学入門

    2022
    Institution name:新潟大学

  • Topics in Food Sciences

    2022
    Institution name:新潟大学

  • 応用生命・食品科学セミナーIII

    2022
    Institution name:新潟大学

  • 「食べる」

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIIb

    2021
    -
    2023
    Institution name:新潟大学

  • スタディ・スキルズAIb

    2021
    -
    2023
    Institution name:新潟大学

  • スタディ・スキルズAIa

    2021
    -
    2022
    Institution name:新潟大学

  • スタディ・スキルズAIIc

    2021
    -
    2022
    Institution name:新潟大学

  • スタディ・スキルズAIc

    2021
    -
    2022
    Institution name:新潟大学

  • スタディ・スキルズAIIa

    2021
    -
    2022
    Institution name:新潟大学

  • 食品素材機能論

    2021
    -
    2022
    Institution name:新潟大学

  • 食品科学セミナー

    2021
    Institution name:新潟大学

  • 農学入門II

    2021
    Institution name:新潟大学

  • 農学入門I

    2021
    Institution name:新潟大学

  • 水産食品学

    2020
    Institution name:新潟大学

  • 食品科学概論

    2020
    -
    2022
    Institution name:新潟大学

  • 産業スペシャリスト育成特論

    2020
    -
    2021
    Institution name:新潟大学

  • 食の安全・安心論

    2020
    Institution name:新潟大学

  • 卒業論文Ⅱ

    2020
    Institution name:新潟大学

  • 卒業論文Ⅰ

    2020
    Institution name:新潟大学

  • 食品安全学

    2019
    Institution name:新潟大学

  • 農学入門Ⅱ

    2019
    Institution name:新潟大学

  • 農学入門Ⅰ

    2019
    Institution name:新潟大学

  • 地域交流サテライト実習

    2019
    Institution name:新潟大学

  • 生命を知る

    2018
    Institution name:新潟大学

  • 基礎生物学

    2017
    Institution name:新潟大学

  • 食と健康の科学

    2017
    Institution name:新潟大学

  • 公衆衛生学Ⅱ

    2017
    -
    2019
    Institution name:新潟大学

  • Topics in Food Sciences

    2016
    -
    2022
    Institution name:新潟大学

  • 公衆衛生学Ⅰ

    2016
    -
    2020
    Institution name:新潟大学

  • 水産製造学

    2016
    -
    2018
    Institution name:新潟大学

  • 生命・食料科学博士特定研究Ⅲ

    2015
    Institution name:新潟大学

  • 外国語論文解説・討論Ⅲ

    2015
    Institution name:新潟大学

  • 基礎生命科学(博士)演習(中間発表)

    2015
    Institution name:新潟大学

  • 生命・食料科学博士セミナーⅢ

    2015
    Institution name:新潟大学

  • 生命・食料科学博士特定研究Ⅰ

    2014
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学博士セミナーⅡ

    2014
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学博士セミナーⅠ

    2014
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学博士特定研究Ⅱ

    2014
    -
    2015
    Institution name:新潟大学

  • 外国語論文解説・討論Ⅱ

    2014
    Institution name:新潟大学

  • 外国語論文解説・討論Ⅰ

    2014
    Institution name:新潟大学

  • 応用生命・食品科学概論

    2012
    -
    2020
    Institution name:新潟大学

  • 応用生命・食品科学特論

    2012
    -
    2020
    Institution name:新潟大学

  • 「食べる」

    2012
    -
    2020
    Institution name:新潟大学

  • 研究発表演習(中間発表)

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅱ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究BⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅡ

    2012
    -
    2015
    Institution name:新潟大学

  • 文献詳読Ⅰ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーBⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 応用生命・食品科学演習(学会発表)

    2012
    -
    2014
    Institution name:新潟大学

  • 実践型食づくりプロジェクト

    2011
    Institution name:新潟大学

  • 食品・栄養科学演習Ⅰ

    2011
    -
    2019
    Institution name:新潟大学

  • 食品・栄養科学演習Ⅱ

    2011
    -
    2018
    Institution name:新潟大学

  • 企画提案型食づくりプロジェクト

    2011
    -
    2014
    Institution name:新潟大学

  • 食=「人」に「良」

    2011
    Institution name:新潟大学

  • 食品・栄養科学実験

    2010
    -
    2018
    Institution name:新潟大学

  • 実地見学

    2010
    -
    2016
    Institution name:新潟大学

  • 自然科学総論Ⅳ

    2010
    -
    2014
    Institution name:新潟大学

  • 食品化学

    2007
    Institution name:新潟大学

  • 食品衛生学

    2007
    Institution name:新潟大学

  • 食品生化学

    2007
    Institution name:新潟大学

  • 生物化学実験

    2007
    -
    2022
    Institution name:新潟大学

  • 食品素材機能論

    2007
    -
    2022
    Institution name:新潟大学

  • くらしと微生物

    2007
    -
    2016
    Institution name:新潟大学

  • 生物学

    2007
    -
    2015
    Institution name:新潟大学

  • 新潟発『食べる』

    2007
    -
    2010
    Institution name:新潟大学

  • 免疫・血清学

    2007
    Institution name:新潟大学

  • 食品製造学

    2007
    Institution name:新潟大学

  • 公衆衛生学II

    2007
    Institution name:新潟大学

▶ display all