記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本醸造協会  

佐瀬式および薮田式でのカプロン酸エチル高生産性酵母を使用した吟醸醪の上槽時の圧力増加に伴う，製成酒の香気成分と遊離脂肪酸の挙動を調査した。佐瀬式および薮田式では，上槽圧力の増加に伴い，香気成分は減少し，遊離脂肪酸は増加する傾向にあり，特に佐瀬式の「責め」部分の製成酒の酢酸イソアミルおよびカプロン酸エチルの減少が顕著であった。これらの結果から，上槽圧力が高い区分の製成酒は，上槽初期に得られる製成酒の香気バランスをそのまま維持することは難しいことが示唆された。

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その他リンク： http://id.ndl.go.jp/bib/031200436

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本醸造協会  

1548年に創業した吉乃川株式会社の酒母及び醪から，TTC染色法とK7_02212遺伝子およびPPT1遺伝子を標的とするLAMP法をスクリーニング法として利用し，6×10 3株の酵母より，K7_02212遺伝子とPPT1遺伝子の両方を有するK7グループ系清酒酵母とは異なるYS4株を分離した。YS4株の生理学的特徴(TTC染色性，酸性ホスファターゼ活性，細胞膜表面の疎水度，胞子形成率)が，K7グループ以外のきょうかい清酒酵母とも異なることが強く示唆されたため，蔵付き酵母と判断した。また，YS4株はSaccharomyces cerevisiaeとして同定された。実規模醸造試験の結果，YS4株からは酸味に特徴をもった酒質の清酒が得られることがわかった。

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その他リンク： http://id.ndl.go.jp/bib/030663322

記述言語：日本語   出版者・発行元：公益社団法人 日本食品科学工学会  

糠を有効に活用して米の機能性を向上させるために，玄米を原料に3種類の麹菌<i>Aspergillus oryzae</i>（AO），<i>Aspergillus sojae</i>（AS），<i>Aspergillus luchuensis</i>（AL）の種麹を用いて玄米麹と玄米塩麹を調製し，抗酸化活性を調べた．3種類の玄米麹の抗酸化活性は玄米より高く，中でもAL玄米麹で最も高かった．また，玄米塩麹の抗酸化活性は玄米より高いが玄米麹に比べ低かった．米に含まれる代表的抗酸化成分であるフェルラ酸を定量したところ，AL玄米麹はフェルラ酸を玄米の約250倍多く含み，これが玄米麹の抗酸化活性が高い一因であることが示された．また，そのフェルラ酸含量はAL白米麹に比べ1.3倍多かった．米中のフェルラ酸の大部分は細胞壁に結合して存在し，フェルロイルエステラーゼ（FAE）の作用により遊離する．そこで3種類の玄米麹のFAE活性を測定した．その結果，AL玄米麹で著しく高い活性が検出され，玄米麹のフェルラ酸含量の結果と一致した．一方，AL玄米塩麹のフェルラ酸含量はAL玄米麹より低く，ALのFAEが食塩により阻害されたことから，玄米塩麹のフェルラ酸が少ない理由は塩麹の発酵中にFAEが失活するとともにフェルラ酸が分解されたためと推察された．以上より，玄米をFAE産生能が高い麹菌で発酵させることにより，白米を用いた時に比べ，機能性成分であるフェルラ酸を豊富に含む麹を調製できることが明らかにされた．

DOI： 10.3136/nskkk.67.236

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その他リンク： https://www.jstage.jst.go.jp/article/nskkk/67/7/67_236/_pdf

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本醸造協会  

S. cerevisiaeのFDC1遺伝子中にp. Lys54Terを生じさせる160番塩基の変異(c. 160A>T)をミスマッチプライマーを用いたPCR-RFLP法により検出する方法を開発した。本法によって，FDC1遺伝子の変異(c. 160A>T)に起因する酵母のフェルラ酸脱炭酸能の有無を識別することができる。本法は迅速且つ信頼性の高い方法であり，今後清酒醸造用酵母の分離・育種において活用が期待される。

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その他リンク： http://id.ndl.go.jp/bib/030420156

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本醸造協会  

DetectX Urea Nitrogen Colorimetric Detection Kit(Arbor Assays社)を用いた清酒中の尿素定量について検討した。このキットは，清酒中に含まれるエタノールや有機酸の影響を受けずに尿素の定量が可能であり，測定精度も良好であった。市販清酒中の尿素を測定した結果，ジアセチルモノオキシム(DAMO)法と比較して相関性のある結果が得られ，簡便かつ迅速な尿素の定量分析法として確立できた。さらに，本キットを用いて，ウレアーゼによる清酒の尿素低減処理の成否を，目視で確認できることがわかった。また，適切な反応時間の検討が必要だが，様々な温度で呈色することを確認した。これらの結果から，本キットによる分析は，清酒製造に適した尿素分析法であり，日常的な分析法として実用性の高い手法である。

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その他リンク： http://id.ndl.go.jp/bib/030266241

記述言語：日本語   出版者・発行元：日本食物繊維学会  

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その他リンク： https://search.jamas.or.jp/link/ui/2020209331

掲載種別：研究論文（学術雑誌）   出版者・発行元：The Mycological Society of Japan  

DOI： 10.1016/j.myc.2019.02.005

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記述言語：日本語   出版者・発行元：公益財団法人 日本醸造協会  

近年，消費者の食の安心・安全に対する関心が高まり，食品メーカーには自社製品に関する消費者からの問い合わせが多く寄せられている。そのような問い合わせに対しては科学的根拠を元に対応することが求められるが，中小清酒メーカーでは困難な場合も多い。本稿では，無機成分の比色定量キットを用いた清酒の簡易識別法について解説していただいた。本法は，中小清酒メーカーでも応用可能であるので，参考にしていただきたい。

DOI： 10.6013/jbrewsocjapan.114.268

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記述言語：日本語   出版者・発行元：兵庫県立コウノトリの郷公園  

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記述言語：日本語   出版者・発行元：新潟大学教育・学生支援機構  

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記述言語：英語   出版者・発行元：Mycological Society of Japan ; 1994-  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：TAYLOR & FRANCIS LTD  

Alzheimer's disease and type 2 diabetes are very serious diseases with the latter having been suggested to cause the former. We prepared super-hard rice bread blended with black rice bran (SRBBB), which contained a high amount of resistant starch that showed strong inhibitory activities against beta-secretase and acetylcholinesterase even after heating. Black rice bran showed greater beta-secretase inhibitory activity (3.6-fold) than Koshihikari rice. The bran contained more oleic acid and anthocyanin, meaning that it is potentially a biofunctional food with a high antioxidant capacity. Furthermore, aged mice, which were fed a SRBBB diet for four weeks, showed lower amyloid beta 40 peptide in the blood than mice fed a commercial diet (p &lt; 0.01). Additionally, their initial blood glucose levels (BGLs) after 12 weeks of being fed SRBBB were significantly lower than those in the control group. Taken together, our results indicate SRBBB shows promise for inhibiting not only amyloid beta production, but also abrupt increases in postprandial BGLs.

DOI： 10.1080/09168451.2016.1240605

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Stable hydrogen and oxygen isotopic compositions (δD and δ18O values, respectively) were analyzed for "sake," a traditional Japanese alcohol beverage, to assess these values for the identification of its geographic origin. We collected sake (Junmai-shu; made of only rice, water, and koji) and its source water (i.e., brewing water) from breweries in Niigata Prefecture, Japan, and measured the isotopic compositions of water in these samples. The δD and δ18O values for the sake are well correlated with their respective values for the corresponding brewing water (δD; r = 0.92, δ18O; r = 0.80). Furthermore, based on the δD-δ18O cross plot, sake brewed in Niigata Prefecture is distinguishable from that brewed in countries other than Japan. These results imply that this dual isotope (δD and δ18O) analysis is potentially useful in identifying the geographic origin of sake.

DOI： 10.2116/analsci.33.979

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE BV  

Here, we describe C8-222, a novel cerulenin-resistant strain of Saccharomyces cerevisiae Meijo-9 that encodes a point mutation (FAS2-1253A mutation) resulting in a Gly to Ala substitution at position 1253 of the fatty acid synthase alpha subunit (Fas2 protein), which was isolated by traditional screening in the presence of cerulenin. Notably, this mutation yielded enhanced production of caprylic acid, but not of caproic acid. As caprylic acid is a precursor of ethyl caprylate, a compound that confers "fruity-flowery" flavors, usage of this mutant may enable the production of new types of sake. (C) 2016 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.

DOI： 10.1016/j.myc.2016.02.004

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Several colorimetric methods were combined and used for the discrimination of commercial sake samples, based on their constituent inorganic components. The method was very rapid, simple, and did not require expensive equipment. Further, we showed that this method has potential application in immediate differentiation of sake by the visual inspection.

DOI： 10.1016/j.jbiosc.2015.05.004

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN SOC FOOD SCIENCE TECHNOLOGY  

gamma-Aminobutyric acid (GABA) was found to inhibit both IgE-mediated and ionomycin-induced histamine release from rat basophilic leukemia RBL-2H3 cells and rat peritoneal exudate cells (PEC) dose-dependently at concentrations between 10 and 1000 mu mol/L. At the same concentrations, baclofen, a GABA(B) receptor agonist, also inhibited both IgE-mediated and ionomycin-induced histamine release from RBL-2H3 cells and PEC. These results suggest that GABA suppresses degranulation in basophils and mast cells via the GABA(B) receptor on the cell surface.

DOI： 10.3136/nskkk.61.362

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記述言語：日本語   出版者・発行元：社団法人 におい・かおり環境協会  

<p>キノコにおいて香りは美味しさを構成する大事な要素である．そのために多くのキノコにおいて香気成分が分析されている．キノコの中にはマツタケや干しシイタケのように特徴香を持つものもあるが，大部分のキノコにおける香りの主成分は1-オクテン-3-オールや1-オクテン-3-オン，3-オクタノン，3-オクタノールなどの揮発性C8化合物である．C8化合物の生合成には，脂質過酸化酵素，過酸化脂質開裂酵素，酸化還元酵素が関与すると考えられているが，よくわかっていない部分も多い．そこで本稿ではキノコの香気とその生合成に関わる酵素について述べる．</p>

DOI： 10.2171/jao.44.315

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KARGER  

We investigated 29 Lactobacillus strains isolated from Japanese traditional fermented food products made from rice grain or bran in order to select strains that potently suppress IgE production. First, in vitro screening was carried out to select candidates with strong induction activity for IL-12 and INF-gamma production and suppression activity for IL-4 production in mouse splenocytes. Based on the screening data, 3 strains were selected and orally administrated to ovalbumin (OVA)-immunized mice. Among these 3 strains, Lactobacillus paracasei K71 (L. paracasei K71) isolated from sakekasu (sake lees) was shown to be the most potent for decreasing total and OVA-specific IgE levels in serum. L. paracasei K71 may down-regulate IgE production via the induction of Th1 responses and suppression of Th2 responses in vivo.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：TAYLOR & FRANCIS LTD  

The full-length cDNA of the gene PoLOX1 encoding a lipoxygenase (LOX) and its corresponding genomic DNA were isolated from the basidiomycete mushroom Pleurotus ostreatus strain H1. The deduced amino acid sequence of PoLOX1 showed similarity to a valencene dioxygenase of Pleurotus sapidus, putative LOX-like proteins from ascomycete, basidiomycete, and deuteromycete fungi, and known LOXs from plants, animals, and bacteria. PoLOX1 also contained the LOX iron-binding catalytic domain in the C-terminal region, but not the polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain, which is usually found in the N-terminal region of eukaryotic LOXs. Genomic sequence analysis revealed that PoLOX1 was interrupted by one intron, and that the promoter region included TATA and CAAT boxes. Southern blot analysis indicated that PoLOX1 is a member of a small gene family comprising highly similar genes. Northern blot analysis revealed that it is transcribed more abundantly in the stipes of the fruit bodies than in the caps.

DOI： 10.1271/bbb.120484

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記述言語：日本語   出版者・発行元：日本作物学会  

紫黒米の生産現場で一定量発生する粒厚1.85 mm未満の米粒（一般には篩い下米あるいは屑米と称される）を，米加工食品産業界へ安価に提供することを前提とし，新潟県が開発した紫黒糯米品種「紫宝」を用い，粒厚別にポリフェノール含量と熱糊化特性を調査して，その加工特性を評価した．「紫宝」の米粒1粒あたりのポリフェノール含量は，粒厚1.85 mm以上の精玄米に比べ，1.85～1.70 mmの米粒で26.1％，1.70 mm未満の米粒で54.3％低下した．したがって，粒厚1.85 mm未満の篩い下米を一般食用米に添加して利用する場合，精玄米並のポリフェノール含量を確保するためには，通常量より30～50％程度増量する必要があると考えられた．単位重量あたりに換算した場合，1粒あたりより，ポリフェノール含量の低下率が低く，篩い下米を米粉として利用する場合には，10～25％程度の増量で精玄米並のポリフェノール含量を確保できると考えられた．一方，玄米のタンパク質含有率は，粒厚が薄くなるにつれて高まり，粒厚1.70 mm未満の米粒では8.6％に達した．また，精玄米に比べ，粒厚1.85 mm未満の篩い下米の糊化開始温度および各種粘度特性値は低く，餅生地の硬化性や澱粉の膨潤能，ゲル強度，老化性は低いと推定され，加工利用時に注意を要すると考えられた．生産現場で一定量発生する篩い下米を原材料として米加工食品産業界に提供するためには，こうした加工利用上の留意点を製造現場にあらかじめ十分に伝達すること，生産流通体制を整備することが重要と考えられた．

DOI： 10.1626/jcs.82.192

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その他リンク： https://www.jstage.jst.go.jp/article/jcs/82/2/82_192/_pdf

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）  

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：フレグランスジャーナル社  

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society of Material Cycles and Waste Management  

新潟県胎内市の家庭生ごみを150℃で40分間，または200℃で15分間蒸煮し飼料化を試みた。蒸煮後の生ごみは，粗蛋白質含量および粗脂肪含量が肥育ブタ用配合飼料に比べて高く，それぞれ18～23%および10～17%であった。一方，ペプシン消化率は低く，26～56%であった。蒸煮生ごみの有効性を評価するため，体重約70kgの肥育ブタ8頭を4頭ずつ試験群と対照群の2群に分け，給与試験を行った。試験群では，まず200℃で蒸煮した生ごみ (200℃生ごみ) を配合飼料に20%混合して29日間給与し，その後200℃生ごみの代わりに150℃で蒸煮した生ごみ (150℃生ごみ) を混合して体重が110±5kgに達するまで給与した。対照群には100%配合飼料のみを給与した。試験群の4頭中2頭は，試験期間を通じて対照群のブタと同等の日増体重を示した。他の2頭は，200℃生ごみ混合飼料を給与した期間に日増体重が低下したものの，その後回復し，対照群のブタに比べて29∼46日遅れて110±5kgに達した。

DOI： 10.3985/jjsmcwm.22.253

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00377522808?from=CiNii

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER TOKYO  

Hydrophobins secreted by filamentous fungi self-assemble into an amphipathic film at hydrophilic/hydrophobic interfaces. This unique property suggests that the hydrophobins have a high potential for industrial applications. However, the assemblages of class I hydrophobins are highly insoluble, making such commercial applications difficult. To enhance the solubility of class I hydrophobins, we have attempted to express class I hydrophobin PNH1 from Pholiota nameko fused with glutathione S-transferase (GST) in Escherichia coli. The GST-PNH1 was effectively isolated from the soluble fraction of transformed E. coli, and subsequent analysis revealed that the purified GST-PNH1 had almost the same emulsifying activity as PNH1.

DOI： 10.1007/s10267-010-0097-9

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3136/nskkk.58.173

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1626/jcs.79.518

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記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.9.157

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.8.295

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

Twenty-three polypeptides secreted in response to a deficiency of inorganic phosphate (Pi) were previously found by two-dimensional polyacrylamide gel electrophoresis analysis in mycelia of Pholiota nameko strain N2. In this study, N-terminal sequencing revealed three of them to be identical to known acid phosphatases of P. nameko strain N114. Two cDNAs and the corresponding genomic DNAs of genes PNAP1 and PNAP2 which encode two of the three acid phosphatases were cloned. The deduced amino acid sequences of PNAP1 and PNAP2 showed high similarity to other fungal acid phosphatases and contained a putative catalytic active site of acid phosphatase. PNAP1 and PNAP2 are comprised of five and seven exons interrupted by four and six introns, respectively. Their promoter regions include two cis-acting elements found in Pi deficiency-inducible genes of Saccharomyces cerevisiae, together with several known functional elements such as a TATA box. Northern blot analysis showed that PNAP1 and PNAP2 are expressed in response to a deficiency of Pi.

DOI： 10.1007/s00294-006-0058-1

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.7.189

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記述言語：日本語   出版者・発行元：日本食品工学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.6.151

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.6.189

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.6.197

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記述言語：英語   掲載種別：研究論文（その他学術会議資料等）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER-VERLAG  

We previously isolated 31 cDNAs corresponding to pdi [inorganic phosphate (Pi) deficiency-inducible] genes through the differential screening of a cDNA library constructed from the mycelium of Pholiota nameko strain N2 cultured in Pi-depleted medium. Among the cDNAs, pdi251, pdi263 and pdi315 were analyzed here. The deduced amino acid sequences of pdi251, pdi263 and pdi315 showed high similarity to fungal hydrophobins and genes corresponding to these cDNAs encoding P. nameko hydrophobins were designated pnh1, pnh2 and pnh3, respectively. PNH1, PNH2 and PNH3 had a conserved spacing of eight cysteine residues in hydrophobins and a hydropathy pattern characteristic of class I hydrophobins. Phylogenetic analysis showed that PNH1, PNH2 and PNH3 were phylogenetically similar and significantly related to the hydrophobin POH1 specifically expressed in fruiting bodies of Pleurotus ostreatus. Northern blot analysis indicated that, under conditions of Pi deficiency, pnh2 and pnh3 were also induced in strains N4 and N301.

DOI： 10.1007/s00294-003-0457-5

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER-VERLAG  

Thirty-one cDNAs corresponding to pdi genes [inorganic phosphate (Pi) deficiency-inducible genes] were previously isolated through the differential screening of a cDNA library constructed from the mycelium of Pholiota nameko. Among the cDNAs, pdi370 was analyzed here. The deduced amino acid sequence showed high similarity to fungal ribonucleases (RNases) and contained two signature sequences conserved in T2 family RNases: CAS1 and CAS2. Genomic DNA harboring the pdi370 gene was isolated from a genomic library of P. nameko. Sequence analysis showed that the pdi370 gene is interrupted by 16 introns and that the promoter region contains two cis-acting sequences found in Pi deficiency-induced genes from Saccharomyces cerevisiae, together with several known functional elements, such as a TATA box. RNase activity in the mycelium and culture filtrate increased 5.6-fold and 5.2-fold, respectively, under Pi-deficient conditions. Staining for RNase activity showed that at least four RNases are induced and secreted under the conditions. The N-terminal sequence of one of them agreed with that of the pdi370 gene product.

DOI： 10.1007/s00294-003-0463-7

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3136/nskkk.51.123

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.5.121

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.11301/jsfe2000.5.235

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：S. Karger AG  

The participation of lipase in the formation of n-hexanal by homogenization of alfalfa seedlings was investigated. n-Hexanal was increased by the addition of trilinolein, dilinolein or monolinolein to the homogenate of the seedlings. Taurodeoxycholic acid sodium salt (TDCA), an inhibitor of triacylglycerol lipase, inhibited the formation of n-hexanal by 36%. These findings show that the corresponding proportion of n-hexanal was formed through the action of triacylglycerol lipase. n-Hexanal was also increased by adding the total lipids of alfalfa seedlings to the homogenate prepared with TDCA. But, when the lipids removed free fatty adds from the total lipids were added to the homogenate, the increase decreased to 18% as compared with the addition of total lipids. n-Hexanal thus also increased through the pathway, not requiring the action of the lipase and 82% of the increased n-hexanal was formed from preexisting free fatty acids. The formation pathways of n-hexanal in the seedlings were discussed.

DOI： 10.3136/fstr.9.185

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER CHEMICAL SOC  

Lipoxygenase was purified homogeneously from cups of Pleurotus ostreatus by Sephacryl S-400 HR gel filtration, Dyematrex Green A affinity, and DEAE-Toyopearl 650M ion-exchange chromatographies. The molecular weight of the enzyme was estimated to be 67 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 66 000 by gel filtration; the isoelectric point was pH 5.1. The optimum pH and temperature of the enzymatic activity were 8.0 and 25 degreesC, respectively. The enzyme contained non-heme iron, and a thiol group seemed to be involved in its activity. The K-m, V-max and k(cat) values of the enzyme for linoleic acid were 0.13 mM, 23.4 mumol(.)min(-1.)mg(-1), and 25.7 s(-1), respectively. The enzyme showed high specificity toward linoleic acid. When linoleic acid was incubated with the enzyme, 13-hydroperoxy-9Z, 11E-octadecadienoic acid was found to be the main oxidative product.

DOI： 10.1021/jf0112217

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The effects of Pi deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)+ RNA isolated from mycelia cultured in Pi-depleted (P−) media, and 150 clones corresponding to Pi deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)+ RNAs from the mycelia cultured in the Pi-supplied (P+) and P− media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during Pi deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters. © 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

DOI： 10.1271/bbb.66.790

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：S. Karger AG  

Sensory test of the grassy odor with 2,3, 5 and 7-day-old seedlings of alfalfa showed that 2-day-old seedlings are the grassiest To identify the substance responsible for the grassy odor, the composition of fatty acid and the characteristics of lipoxygenase were investigated. The results showed that more than 50% of total fatty acid is linoleic acid
lipoxygenase has a high specificity to linoleic acid and produces mainly 13-Z, E-HPOD from linoleic acid. Because 13-Z, E-HPOD is converted to n-hexanal which has a grassy odor, the content of n-hexanal in the seedlings after germination was measured. It was found that it did not change remarkably throughout the tested growth period. Most of the n-hexanal was adsorbed weakly to insoluble materials with hydrophobic interaction. However, when the seedlings were homogenized, soluble n-hexanal increased rapidly and the increase was greatest in 2-day-old seedlings. Among three enzymes involved in the formation of n-hexanal, only the change in lipoxygenase activity correlated with that in the increase of n-hexanal.

DOI： 10.3136/fstr.8.347

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Mycological Society of Japan  

Changes in mycelial dry weight and soluble protein amounts and acid phosphatase activities on a mycelial dry weight basis in the mycelia and culture supernatants during the Pi-supplied (P+) and Pi-depleted (P-) cultures of three strains of Pholiota nameko were examined. Mycelial dry weights of the three strains were lower in the P- culture than in the P+ culture. However, soluble protein amounts in the culture supernatants and acid phosphatase activities in the mycelia and culture supernatants of the three strains were higher on a mycelial dry weight basis in the P- culture than in the P+ culture. Total proteins of strains N2 and N4 were analyzed by two-dimensional-PAGE. Comparison of electrophoretograms of the P+ and P- cultures showed that many polypeptides in the two strains were induced and secreted by Pi deficiency, but more than half of them were specific to each strain. Activity staining of acid phosphatase also revealed that two isozymes with the same molecular weights in the three strains were induced and secreted by Pi deficiency. Adaptive mechanisms for Pi deficiency in the three strains were discussed.

DOI： 10.1007/BF02460947

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Changes in activity of RNA-degrading enzyme and amounts of phosphorus in mycelia and culture filtrate during Pi-sufficient and -deficient cultures of Pholiota nameko were investigated. The results showed that the intracellular and extracellular activities are controlled by the Pi concentration in the medium. Moreover, the induction and secretion of RNA-degrading enzymes under the Pi-deficient condition were analyzed by activity staining.

DOI： 10.1271/bbb.65.2561

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記述言語：日本語   出版者・発行元：新潟大学農学部  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate (InsP6). The pathway of hydrolysis of InsP6by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a variety of nuclear magnetic resonance techniques (1H-, two-dimensional1H-1H coupling-correlation spectra and two-dimensional31P-1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP6to yield D/L-Ins (1, 2, 3, 5, 6) P5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1, 2, 5, 6) P4, D/L-Ins (1, 2, 6) P3and D/L-Ins (1, 2) P2, while the minor route proceeds via D/L-Ins (1, 2, 3, 6) P4, Ins (1, 2, 3) P3and D/L-Ins (1, 2) P2. D/L-Ins (1, 2) P2is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

DOI： 10.1271/bbb.64.995

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The nucleotide sequence of hiC12, isolated as a cDNA clone of hardening-induced Chlorella (hiC) genes, was identified. The clone encodes a late embryogenesis abundant (LEA) protein having six repeats of a 11-mer amino acid motif, although in a slightly imperfect form. To overexpress the hiC61) and hiC12 genes, their coding regions were PCR amplified and subcloned into a pGEX-1λT vector. The HIC6 and HIC12 proteins were expressed as GST fusion proteins inE. coli, then purified. The two HIC proteinswere found to be effective in protecting a freeze-labile enzyme, LDH, against freeze-inactivation. On a molar concentration basis, they were about 3.1×106 times more effective in protecting LDH than sucrose and as effective as BSA. Cryoprotection tests with five kinds of chain-shortened polypeptides, synthesized based on the 11-mer amino acid motif of the HIC6 protein showed that the cryoprotective activity decreased with a decrease in the repeating units of the 11-mer motif. In fact, cryoprotective activities of three kinds of single 11-mer amino acids were very low even at high concentrations. All the results suggested that the sufficiently repeated 11-mer motif is required for the cryoprotective activities of Chlorella LEA proteins. © 2000, Taylor &amp
Francis Group, LLC. All rights reserved.

DOI： 10.1271/bbb.64.1656

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記述言語：日本語   出版者・発行元：Japanese Society for Food Science and Technology  

米菓製造工程産物及び米菓製造模擬工程産物の熱分析(示差走査熱量測定)を行い,米菓製造工程とその工程中に生じている糊化・老化澱粉ゲルの挙動を次のステップで解析した.<BR>(1) 実際の糯米菓製造工程産物のDSC測定を行った.<BR>(2) 糯米粉を使用した糯米菓製造模擬工程産物のDSC測定を行った.<BR>(3) 糯米澱粉を使用した糯米菓製造模擬工程産物のDSC測定を行った.<BR>その結果,米菓製造工程における蒸し工程は,澱粉の糊化現象による水素結合の崩壊のプロセスである.冷蔵工程は,老化現象による再配列構造の生成すなわち新たな水素結合の生成のプロセスである.乾燥工程はアニーリングによる,さらに高温側に安定な再配列構造の生成のプロセスである.焙焼工程は再配列構造の崩壊のプロセスである.さらに各々の工程はお互いに関係しており,糯米菓の最終製品に影響することが推測される.従って,熱分析法を用いて,米菓製造工程とその工程中に生じている糊化・老化澱粉ゲルの挙動を解析することが可能であると結論できる.

DOI： 10.3136/nskkk.46.51

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その他リンク： https://jlc.jst.go.jp/DN/JALC/00059339519?from=CiNii

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：S. Karger AG  

Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration chromatography, DEAE-TOYOPEARL chromatography, CM-TOYOPEARL chromatography and second Sephacryl S-200 HR gel filtration chromatography. Molecular weights of the two enzymes were 71,000 and 66,000 by gel filtration, and 68,000 and 66,000 by SDS-PAGE, respectively. Optimum pH and temperatures were 6.0 and 45°C for PHY1, and 5.5 and 50°C for PHY2. The activity of both phytases was stable at pHs between pH 4.0-7.0 and below 40°C. The Km values for myo-inositol hexakisphosphate (IHP) were 0.48 μM for PHY1 and 0.77 μM for PHY2. The Ki values for Pi were 2.69 mM for PHY1 and 6.59 mM for PHY2. Both phytases showed relatively high specificity for IHP.

DOI： 10.3136/fstr.5.18

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本食品科学工学会  

This study was conducted to determine the reason why dried rice cakes for non-glutinous rice crackers (Senbei) often break in the production process. Breakage after drying at 40°C for 12 h was found to be due to milling and storage conditions of the rice flour. Gel permeation chromatography revealed that the molecular weight of starch decreased as the extent of breakage increased. Damage to rice flour starch differed according to the milling methods. When press roller milled rice flour was stored at 5-25°C, the activity of α-amylase increased as the temperature became higher. The total water-soluble carbohydrate content of dried rice cake may be a good criterion to judge the extent of breakage. Reduction of the molecular sizes of starch, due to milling and action of α-amylase, is considered to cause the breakage of dried rice cake.

DOI： 10.3136/fsti9596t9798.4.121

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Mycological Society of Japan  

Mycelial soluble proteins of Pholiota nameko labeled in vivo during the Pi-supplied (P+) and the Pi-depleted (P-) cultures were separated by SDS-polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis, and visualized by fluorography. A comparison of protein profiles from the P+ and P- cultures showed that Pi deficiency induces the synthesis of 15 polypeptides and an increase in the relative amount of 29 polypeptides. These result suggests that many proteins may be specifically synthesized de novo under Pi deficiency as part of the adaptive mechanism for this condition.

DOI： 10.1007/BF02464059

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

An acid phosphatase with a very high substrate specificity for glucose-1-phosphate was isolated for the first time from mycelia of Pholiota nameko. The molecular weight of the enzyme was estimated to be 31,000 on gel filtration and 35,000 on SDS-PAGE. The activity was inhibited by Cu2+, Hg2+, molybdate, and tartaric acid. The sequence of N-terminal 20 amino acid residues was analyzed. © 1998, Taylor &amp
Francis Group, LLC. All rights reserved.

DOI： 10.1271/bbb.62.2251

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記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3136/nskkk.44.353

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Japan  

We previously reported the purification of an acid phosphatase (APase52) secreted from the mycelia of Pholiota nameko under phosphate-deficient conditions. In the present study, two other isozymes (APase47 and APase48) were found and their structures were compared with that of APase52. Thirteen amino acid residues at the N-terminus of APase47 were completely identical with those of APase48 and had partial homology with those of APase52. The deglycosylation of proteins indicated that three APase isozymes differ in the N-linked oligosaccharide content. The protease-generated peptide maps of the APases differed from one another in the band pattern. These results suggest that the APases are the products of different genes.

DOI： 10.1007/BF02464095

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記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：日本菌学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/BF02461313

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Japan  

Activity of acid phosphatase secreted by mycelia of Pholiota nameko on cultivation for 30d in Pi-depleted medium was 88-fold higher than the corresponding activity in the Pi-supplied medium. One isozyme of the secreted acid phosphatases was purified from the culture filtrate of Pi-depleted medium by ammonium sulfate fractionation and cation exchange chromatography. The purified enzyme was homogeneous on electrophoresis. Gel filtration analysis showed that the native molecule had a molecular weight of 117,000. The molecular weight on gel electrophoresis with SDS was 52,000, indicating that the native form of the enzyme was a homodimer. The optimum pH and temperature of the enzyme were 5.5 and 45°C, respectively, and the isoelectric point of the enzyme was pH 6.9. Adsorption on Con A-Sepharose and periodic-Schiff stain suggested that the enzyme is a glycoprotein. The enzyme hydrolyzed a wide variety of phosphate esters, nucleoside phosphates, sugar phosphates, and phosphorylated amino acids. Cu2+, Fe2+, Hg2+, iodoacetate, molybdate, tartaric acid, and SDS inhibited the enzyme activity. Fe3+ (1 mM), Triton X-100, methanol, and ethanol activated it. Fifteen residues of the N-terminal amino acid sequence were determined.

DOI： 10.1007/BF02461459

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid chromatography (2D-HPLC) on an anion-exchange column, with subsequent reversed-phase chromatography. Some of the proteins were resolved by SDS-PAGE, characterized by amino-terminal sequencing and identified by searching for homologies in databases. Separation of the soluble proteins during the hardening of Chlorella by a combination of 2D-HPLC and SDS-PAGE revealed that at least 31 proteins were induced or increased in abundance. Of particular interest was the induction after 12 h of a 10-kDa protein with the amino-terminal amino acid sequence AGNKPITEQISDAVGAAGQKVG and the induction after 6 h of a 14-kDa protein with the amino-terminal sequence ALGEESLGDKAKNAFEDAKDAVKDAAGNVKEAV. The amino-terminal sequences of these proteins indicated that they were homologous to late embryogenesis abundant (LEA) proteins. Furthermore, the level of a 22-kDa protein also increased after 12 h. The amino-terminal sequence of this protein, AAPLVGGPAPDFTAAAVFD, indicated that it was homologous to thioredoxin peroxidase. Copyright © 1995. The Japanese Society of Plant Physiologists.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

To investigate the effects of hardening on gene expression in Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27), a frost-hardy strain, 17 cDNA clones corresponding to hardening-induced Chlorella (hiC) genes were isolated by differential screening of a cDNA library from 6-h hardened cells. Northern blot analysis of transcripts of hiC genes showed that these genes are specifically induced by hardening and that their patterns of induction vary. Southern blots of genomic DNAs from two strains (Chlorella ellipsoidea Gerneck IAM C-102, chilling-sensitive
and C. vulgaris C-27, frost-hardy) of Chlorella indicated that ten hiC clones out of 17 hybridized only with DNA of strain C-27 and the other seven clones hybridized with DNA of both strains. However, of these seven clones, transcripts corresponding to six clones did not accumulate in strain C-102 at low temperatures. The sequence of a deduced protein encoded by the most abundant clone, hiC6, exhibited homology to sequences of Group III LEA (late embryogenesis abundant) proteins and had an amino-terminal amino acid sequence that was similar to the sequences of chloroplast transit peptides. © 1995 The Japanese Society of Plant Physiologists (JSPP).

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

The composition and positional distribution of fatty acids in the polar lipids from 4 strains of Chlorella differing in chilling susceptibility and frost hardiness were analyzed by enzymatic hydrolysis and gas‐liquid chromatography. Analysis of the polar lipids from chilling‐sensitive, chilling‐resistant and chilling‐sensitive revertant strains of Chlorella ellipsoidea IAM C‐102 showed that the sum of palmitic and trans‐3‐hexadecenoic acid in phosphatidylglycerol (PG) is about 60% for the sensitive strains and 53% for the resistant strain. The sum of dipalmitoyl and 1‐palmitoyl‐2‐(trans‐3‐hexadecenoyl) PG as estimated from the positional distribution of their fatty acids, is about 10% in the case of each of the three strains. The contents of unsaturated fatty acids in phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were higher in the resistant than in the sensitive strain. This suggests that unsaturation of fatty acids in not only PG but also PC and PE is involved in chilling sensitivity of Chlorella. On the other hand, lipid changes during the development of frost hardiness of C. ellipsoidea IAM C‐27, a frost hardy strain, were examined. The results showed that fatty acids in most lipid classes are unsaturated in the hardening process but their degree of unsaturation is not greatly different from that of the chilling‐resistant strain, suggesting that not only unsaturation of fatty acids in lipids but also other factors are necessary for the development of frost hardiness. Copyright © 1993, Wiley Blackwell. All rights reserved

DOI： 10.1111/j.1399-3054.1993.tb00155.x

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記述言語：英語   出版者・発行元：九州大学  

Soluble and membrane proteins from Chlorella ellipsoidea Gerneck (IAM C-27) during the early time of hardening were separated by two-dimensional gel electrophoresis and visualized by silver staining. The analysis of soluble protein showed that 14 polypeptides newly appeared and 3 polypeptides increased in abundance within 24 h of cold treatment. Three polypeptides having the molecular weight 80 kDa (PI 5.58), 82 kDa (p1 5.46) and 66 kDa (p1 6.11) were detected after a 6-h or a 9-h hardening and markedly increased as the time of hardening proceeded. The result suggests that they may play an important role for the development of cold hardiness. One small polypeptide (29.8 kDa, p1 4.44) was detectable in membrane fraction of 12 h-hardened cells. Membrane protein of 29.2 kDa (p1 5.05) was pronounced after a 12-h hardening. Two major groups of membrane polypeptide (50.8-55.4 kDa, pI 5.29-5.76 and 50.8-55.4 kDa, p1 6.70-7.10) disappeared after a 3-h hardening and were not observed following a longer hardening period, suggesting the rapid degradation of these membrane polypeptides during cold treatment.

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記述言語：日本語   出版者・発行元：新潟大学  

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記述言語：日本語   出版者・発行元：社団法人日本農芸化学会  

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記述言語：日本語   出版者・発行元：社団法人日本農芸化学会  

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記述言語：日本語   出版者・発行元：社団法人日本農芸化学会  

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記述言語：日本語   出版者・発行元：新潟大学  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap

記述言語：日本語   会議種別：口頭発表（一般）  

researchmap