Updated on 2025/11/07

写真a

 
SUGIYAMA Toshie
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Professor
Faculty of Agriculture Professor
Title
Professor
Other name(s)
Toshie Sugiyama
External link

Degree

  • 博士(学術) ( 1994.3   新潟大学 )

Research Interests

  • Medullary Bone

  • Calcium Metabolism

  • Leg Weakness

  • Eggshell Quality

  • Bone Metabolism

  • Avian

  • Animal Science

Research Areas

  • Life Science / Applied molecular and cellular biology

  • Life Science / Veterinary medical science

  • Life Science / Animal production science

Research History (researchmap)

  • Niigata University   Faculty of Agriculture   Professor

    2018.4

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  • Niigata University   Faculty of Agriculture   Associtae Professor

    2010.7 - 2018.3

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  • North Carolina State University   Department of Marine, Earth, & Atmospheric Sciences   Visiting Assistant Professor

    2007.8 - 2008.3

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    Country：United States

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  • Pennsylvania State University   Department of Biochemistry and Molecular Cell Biology   Visting Researcher

    2004.6 - 2004.9

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    Country：United States

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  • National Institute of Livestock and Grassland Science   Department of Physiology   Invited Researcher

    2000.6 - 2000.7

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  • Pennsylvania State University   Department of Biochemistry and Molecular Cell Biology   Visting Researcher

    1998.2 - 1998.12

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    Country：United States

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  • Niigata University   Faculty of Agriculture   Assistant Professor

    1997.1 - 2010.6

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  • Niigata University   Graduate School of Science and Technology   Research Assistant

    1995.7 - 1996.12

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  • JSPS Research Fellowship for Young Scientists

    1994 - 1995

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Research History

  • Niigata University   Faculty of Agriculture   Professor

    2018.4

  • Niigata University   Faculty of Agriculture Department of Agriculture   Associate Professor

    2017.4 - 2018.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2010.7 - 2018.3

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2010.7 - 2018.3

  • Niigata University   Faculty of Agriculture Department of Agrobiology   Associate Professor

    2010.7 - 2017.3

  • Niigata University   Faculty of Agriculture Department of Agrobiology   Assistant Professor

    2004.4 - 2010.6

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Education

  • Niigata University   Graduate School of Science and Technology   Biosystem Science

    - 1994

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    Country： Japan

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  • Niigata University   Graduate School of Agriculture

    - 1991

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  • Niigata University   Faculty of Agriculture   Department of Animal Science

    - 1989

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    Country： Japan

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Professional Memberships

  • The Japanese Society of Veterinary Science

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  • Japanese Society of Animal Science

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  • Japan Poultry Science Association

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Papers

  • Efficient Isolation and Characterization Methods for Chicken Serum Extracellular Vesicles. International journal

    Hatsune Mukohda, Muhammet Mustafa Binici, Katsuya Miyake, Yoshitaka Sano, Takahisa Yamada, Takumi Terada, Toshie Sugiyama

    Animal science journal   96 ( 1 )   e70092   2025.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    Extracellular vesicles (EVs) are small cell membrane-derived vesicles that are known as mediators of cell-to-cell communication. Efficient methods for chicken serum EV isolation and identification have not been established to satisfy the international guidelines of the Minimal Information for Studies of Extracellular Vesicles (MISEV). Therefore, the present study isolated EVs from chicken serum using four conventional methods-ultracentrifugation (UC), two polymer-based precipitations (PP), and filtration (FL)-and characterized them by western blotting (WB) using polyclonal antibodies (anti-CD9, anti-PDCD6IP, anti-TSG101, and anti-calnexin), nanoparticle tracking analyses, and transmission electron microscopy of MISEV guidelines. As a result, all isolated samples represented specific EV marker proteins, small particles sized 30-150 nm, and spherical morphology with a double membrane structure. In particular, FL-isolated EVs showed the most distinct expression of the marker proteins by WB analysis and the highest expression of miR-451, which is a stable microRNA of EVs, by quantitative PCR, suggesting that FL could isolate highly purified EVs. In conclusion, we established an optimal characterization method for chicken EVs and estimated the efficiency of EV isolation methods from chicken serum.

    DOI： 10.1111/asj.70092

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  • Renal Endocytic Regulation of Vitamin D Metabolism during Maturation and Aging in Laying Hens. International journal

    Nami Kuwata, Hatsune Mukohda, Hiroto Uchida, Ryo Takamatsu, Muhammet Mustafa Binici, Takahisa Yamada, Toshie Sugiyama

    Animals   14 ( 3 )   502   2024.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    Egg-laying hens undergo a specific and dramatic calcium metabolism to lay eggs with eggshells composed of calcium carbonate. Calcium metabolism is mainly regulated by vitamin D3. Although vitamin D3 metabolism is closely related to the deterioration of eggshell quality associated with aging and heat stress, the details of the mechanisms regulating vitamin D3 metabolism are not clear. In mammals, the vitamin D3 metabolite (25(OH)D3) produced in the liver binds to the vitamin binding protein (DBP), is subsequently taken up by renal proximal tubular cells via the endocytic receptors megalin (Meg) and cubilin (CUB), and is metabolized to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Therefore, the present study aimed to examine the expression and localization of Meg and CUB in the kidneys of immature chicks and mature and aged laying hens to prevent eggshell quality deterioration. As a result, we showed that as circulating 1,25(OH)2D3 concentrations increased from 156.0 ± 13.5 pg/mL to 815.5 ± 61.4 pg/mL with maturation in immature chicks, relative expression levels (arbitrary units; AU) of Meg and CUB mRNA in the kidneys of mature hens significantly increased 1.92- and 2.75-fold, respectively, compared to those in immature chicks. On the other hand, the Meg mRNA expression levels of mature hens did not change with age, while CUB mRNA expression levels (1.03 ± 0.11 AU) were significantly decreased compared to mature hens (2.75 ± 0.24 AU). Immunohistochemical observations showed that Meg and CUB proteins were localized to the apical membrane of renal proximal tubular epithelial cells in immature chicks, mature hens, and aged hens, and that DBP protein was observed as granular endosomes in the cytoplasm of proximal tubular cells from the apical membrane to the cell nucleus. Especially in mature hens, the endosomes were larger and more numerous than those in immature chicks. In contrast, in aged hens, DBP-containing endosomes were smaller and limited to the apical cytoplasm. These results indicate that with maturation, the expression of Meg and CUB is promoted in the renal proximal tubules of laying hens, facilitating the uptake of the 25(OH)D3-DBP complex and its conversion to 1,25(OH)2D3, and regulating calcium metabolism in eggshell formation. On the other hand, it is suggested that the age-related decrease in CUB expression suppresses the uptake of the 25(OH)D3-DBP complex in the kidney, resulting in a deterioration of eggshell quality.

    DOI： 10.3390/ani14030502

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  • CD41+ extracellular vesicles produced by avian thrombocytes contain microRNAs. International journal

    Kenkichi Sugimoto, Takamasa Nishikawa, Toshie Sugiyama

    Genes to cells : devoted to molecular & cellular mechanisms   28 ( 12 )   915 - 928   2023.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    Avians have thrombocytes in their blood circulation rather than mammalian platelets. However, many details of thrombocyte characteristics have not been determined. Here, chicken thrombocytes were isolated, and extracellular vesicle (EV) production was investigated. The thrombocyte-specific markers cd41 and cd61 were expressed in the yolk sac at 24 h. According to the embryonic developmental stage, the cd41-expressing tissues changed from the yolk sac to the bone marrow and spleen. Accordingly, the bone marrow and spleen were the main tissues producing thrombocytes in adult chickens. Avian thrombocytes were separated from adult spleen cells through a combination of discontinuous density gradient centrifugation, phagocytic cell removal, and fluorescence-activated cell sorting. Isolated thrombocytes produced CD41+ EVs (CD41+ EVs), and the CD41+ EVs also expressed CD9. Microarray analysis revealed that CD41+ EVs contain many microRNAs. Macrophage lines (RAW264.7) phagocytosed CD41+ EVs, and their phagocytosis and migration activity were suppressed. Microarray analysis also revealed that EVs altered gene expression in macrophages. These data indicated that the CD41+ EV was a carrier of microRNAs produced from thrombocytes and affected the cell characteristics of the received cells. Therefore, the CD41+ EVs of avians worked as a communication tool.

    DOI： 10.1111/gtc.13078

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  • Mating in the cold. Prolonged sperm storage provides opportunities for forced copulation by male bats during winter

    Takahiro Sato, Toshie Sugiyama, Tsuneo Sekijima

    Frontiers in Physiology   14   1241470   2023.9

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    In a wide range of heterothermic mammals, hibernation interrupts the reproductive cycle by forcing reproductive delays. In hibernating bats with delayed fertilization, an opportunity for sperm competition is enhanced by extending a time-window between copulations and fertilization. In order to achieve greater fertilization success, males are expected to show adaptations for sperm competition by increasing their opportunities for mating over an extended period. We aimed to clarify the physiological and behavioral characteristics of male bats experiencing increased risks of sperm competition. We investigated the characteristics of the reproductive cycle of the little horseshoe bat (Rhinolophus cornutus), and examined whether males retain reproductive physiology related to sexual behavior, and attempt to copulate with females even during the hibernation period. Field observations and histological examinations of the reproductive cycle confirmed that females, having mated in the autumn, store spermatozoa in the uterus during hibernation and give birth in the early summer to just one offspring per year, thus males face a low certainty of successful fertilization. Although their testes regressed rapidly and their testosterone levels were lower during winter than in autumn, males stored motile spermatozoa in their cauda epididymides from autumn throughout the winter. During hibernation, we found that males occasionally aroused from torpor and attempted to mate forcibly with torpid females. Forced copulations appear to increase a male’s chances of obtaining a mate while avoiding pre-copulatory female choice. Epididymal sperm storage could be advantageous for males in allowing them to extend their potential mating period even though their testes have regressed. We also found that some hibernating nulliparous females were ready for fertilization in spring after hibernation, whereas few parous females appeared in the same roost. In contrast to males, forced copulations would be maladaptive for females because they cannot opt for higher-quality males while in torpor. Females that have experienced sexual coercion when young may subsequently avoid hibernacula where adult males are present.

    DOI： 10.3389/fphys.2023.1241470

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  • Reduced bone formation and increased bone resorption drive bone loss in Eimeria infected broilers

    Yuguo Hou Tompkins, Janghan Choi, Po-Yun Teng, Masayoshi Yamada, Toshie Sugiyama, Woo Kyun Kim

    SCIENTIFIC REPORTS   13 ( 1 )   2023.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-023-27585-5

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  • Overexpression of miR-125b in Osteoblasts Improves Age-Related Changes in Bone Mass and Quality through Suppression of Osteoclast Formation. International journal

    Shota Ito, Tomoko Minamizaki, Shohei Kohno, Yusuke Sotomaru, Yoshiaki Kitaura, Shinsuke Ohba, Toshie Sugiyama, Jane E Aubin, Kotaro Tanimoto, Yuji Yoshiko

    International journal of molecular sciences   22 ( 13 )   2021.6

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    We recently reported an unexpected role of osteoblast-derived matrix vesicles in the delivery of microRNAs to bone matrix. Of such microRNAs, we found that miR-125b inhibited osteoclast formation by targeting Prdm1 encoding a transcriptional repressor of anti-osteoclastogenesis factors. Transgenic (Tg) mice overexpressing miR-125b in osteoblasts by using human osteocalcin promoter grow normally but exhibit high trabecular bone mass. We have now further investigated the effects of osteoblast-mediated miR-125b overexpression on skeletal morphogenesis and remodeling during development, aging and in a situation of skeletal repair, i.e., fracture healing. There were no significant differences in the growth plate, primary spongiosa or lateral (periosteal) bone formation and mineral apposition rate between Tg and wild-type (WT) mice during early bone development. However, osteoclast number and medial (endosteal) bone resorption were less in Tg compared to WT mice, concomitant with increased trabecular bone mass. Tg mice were less susceptible to age-dependent changes in bone mass, phosphate/amide I ratio and mechanical strength. In a femoral fracture model, callus formation progressed similarly in Tg and WT mice, but callus resorption was delayed, reflecting the decreased osteoclast numbers associated with the Tg callus. These results indicate that the decreased osteoclastogenesis mediated by miR-125b overexpression in osteoblasts leads to increased bone mass and strength, while preserving bone formation and quality. They also suggest that, in spite of the fact that single miRNAs may target multiple genes, the miR-125b axis may be an attractive therapeutic target for bone loss in various age groups.

    DOI： 10.3390/ijms22136745

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  • Effects of early high nutrition related to metabolic imprinting events on growth, carcass characteristics, and meat quality of grass-fed Wagyu (Japanese Black cattle). International journal

    Sithyphone Khounsaknalath, Kotaro Etoh, Kaori Sakuma, Kunihiko Saito, Akira Saito, Tsuyoshi Abe, Fumio Ebara, Toshie Sugiyama, Eiji Kobayashi, Takafumi Gotoh

    Journal of animal science   99 ( 6 )   2021.6

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    The study was conducted to clarify how early high plane of nutrition related to metabolic imprinting affected growth, carcass characteristics, and meat quality of grass-fed Wagyu (Japanese Black cattle). Wagyu steers were allocated randomly into 2 dietary groups: (1) steers fed milk replacer (crude protein 26.0%, crude fat 25.5%; maximum intake 0.6 kg/d) until 3 mo of age and then fed roughage (orchard grass hay) ad libitum from 4 to 10 mo of age (roughage group, RG; n = 11); (2) steers fed milk replacer (maximum intake of 1.8 kg/d) until 3 mo of age and then fed a high-concentrate diet from 4 to 10 mo of age (early high nutrition, EHN; n = 12). After 11 mo of age, all steers were fed roughage ad libitum until 31 mo of age and then slaughtered. Growth performance, carcass traits, longissimus muscle (LM) meat quality and intramuscular fat (IMF) content, plasma insulin-like growth factor I (IGF-I) concentration, and bone mineral density were measured. Body weight was greater in EHN steers (571 kg) than RG steers (520 kg; P < 0.01). Plasma IGF-I levels were higher in EHN steers than in RG steers at 3, 10, and 14 mo of age (P < 0.01, P < 0.005, P < 0.001, respectively); however, plasma IGF-I levels were lower in EHN steers compared with RG steers at 30 mo of age (P < 0.01). The total weight of the muscles and bones of the left half of the carcass was not different between the 2 groups (P = 0.065). Five of the 19 muscles investigated (semimembranosus, P = 0.036; infraspinatus, P = 0.024; supraspinatus, P = 0.0019; serratus ventralis cervicis, P = 0.032; serratus ventralis thoracis, P = 0.027) were heavier in EHN steers. Total fat weight in the left half of the carcass was 30% greater (P = 0.025) in HNE carcasses. Subcutaneous and perirenal fat weights were 53% and 84% greater (P = 0.008, P = 0.002, respectively) in EHN carcasses. The LM IMF content was greater in EHN loins (13.2%) compared with RG loins (9.4%) at 31 mo of age (P = 0.038); however, no differences were found for shear force, tenderness, and cook loss. These results suggested early high-nutrition affected the growth and meat quality of livestock.

    DOI： 10.1093/jas/skab123

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  • Effect of Age on Bone Structure Parameters in Laying Hens

    Masayoshi Yamada, Chongxiao Chen, Toshie Sugiyama, Woo Kyun Kim

    ANIMALS   11 ( 2 )   2021.2

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    DOI： 10.3390/ani11020570

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  • The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice. International journal

    Tomoko Minamizaki, Yuko Nakao, Yasumasa Irie, Faisal Ahmed, Shota Itoh, Nushrat Sarmin, Hirotaka Yoshioka, Asako Nobukiyo, Chise Fujimoto, Shumpei Niida, Yusuke Sotomaru, Kotaro Tanimoto, Katsuyuki Kozai, Toshie Sugiyama, Edith Bonnelye, Yuichiro Takei, Yuji Yoshiko

    Communications Biology   3 ( 1 )   30 - 30   2020.1

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    Communication between osteoblasts and osteoclasts plays a key role in bone metabolism. We describe here an unexpected role for matrix vesicles (MVs), which bud from bone-forming osteoblasts and have a well-established role in initiation of bone mineralization, in osteoclastogenesis. We show that the MV cargo miR-125b accumulates in the bone matrix, with increased accumulation in transgenic (Tg) mice overexpressing miR-125b in osteoblasts. Bone formation and osteoblasts in Tg mice are normal, but the number of bone-resorbing osteoclasts is reduced, leading to higher trabecular bone mass. miR-125b in the bone matrix targets and degrades Prdm1, a transcriptional repressor of anti-osteoclastogenic factors, in osteoclast precursors. Overexpressing miR-125b in osteoblasts abrogates bone loss in different mouse models. Our results show that the MV cargo miR-125b is a regulatory element of osteoblast-osteoclast communication, and that bone matrix provides extracellular storage of miR-125b that is functionally active in bone resorption.

    DOI： 10.1038/s42003-020-0754-2

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  • Salt Tolerance Improvement in Rice through Efficient SNP Marker-Assisted Selection Coupled with Speed-Breeding

    Md Masud Rana, Takeshi Takamatsu, Marouane Baslam, Kentaro Kaneko, Kimiko Itoh, Naoki Harada, Toshie Sugiyama, Takayuki Ohnishi, Tetsu Kinoshita, Hiroki Takagi, Toshiaki Mitsui

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   20 ( 10 )   2019.5

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    DOI： 10.3390/ijms20102585

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  • An estimation of number of birds to be consecutively released in the reintroduction of Japanese Crested Ibises (Nipponia nippon)

    Yuichi Wajiki, Yoshinori Kaneko, Toshie Sugiyama, Takahisa Yamada, Hiroaki Iwaisaki

    WILSON JOURNAL OF ORNITHOLOGY   130 ( 4 )   874 - 880   2018.12

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    DOI： 10.1676/1559-4491.130.4.874

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  • Short communication: Temporal changes in the skin morphology of dairy cows during the periparturient period

    Banri Suzuki, Toshie Sugiyama, Chikako Yoshida, Toshihiko Nakao

    JOURNAL OF DAIRY SCIENCE   101 ( 7 )   6616 - 6621   2018.7

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    DOI： 10.3168/jds.2017-13831

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  • Immunohistohemical Localization of Collagen and Glycosaminoglycans in Chicken Tibial Dyschondroplasia

    Sho IKEDA, Toshie SUGIYAMA

    55   J1 - J7   2018.4

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  • Chemistry supports the identification of gender-specific reproductive tissue in Tyrannosaurus rex International journal

    Mary Higby Schweitzer, Wenxia Zheng, Lindsay Zanno, Sarah Werning, Toshie Sugiyama

    SCIENTIFIC REPORTS   6   23099 - 23099   2016.3

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    DOI： 10.1038/srep23099

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  • Genetic Analyses in the Japanese Captive Population of Japanese Crested Ibis (Nipponia nippon) using Pedigree Information

    Yuichi Wajiki, Yoshinori Kaneko, Toshie Sugiyama, Takahisa Yamada, Hiroaki Iwaisaki

    JOURNAL OF POULTRY SCIENCE   53 ( 1 )   1 - 7   2016.1

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    DOI： 10.2141/jpsa.0150040

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  • Alligator osteoderms as a source of labile calcium for eggshell formation

    C. G. Dacke, R. M. Elsey, P. L. Trosclair, T. Sugiyama, J. G. Nevarez, M. H. Schweitzer

    JOURNAL OF ZOOLOGY   297 ( 4 )   255 - 264   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/jzo.12272

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  • Bone morphological analyses in Spontaneously Diabetic Toni (SDT) fatty rats

    Takeshi Ohta, Shuichi Kimura, Masaya Hirata, Takahisa Yamada, Toshie Sugiyama

    JOURNAL OF VETERINARY MEDICAL SCIENCE   77 ( 10 )   1327 - 1330   2015.10

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    DOI： 10.1292/jvms.15-0154

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  • ASSOCIATION OF THE EXPRESSION LEVEL OF THE MYBPC1 GENE IN SKELETAL MUSCLE WITH MARBLING TRAIT IN JAPANESE BLACK BEEF CATTLE

    Bin Tong, Youji Muramatsu, Takeshi Ohta, Hiroyuki Kose, Hideaki Yamashiro, Toshie Sugiyama, Takashisa Yamada

    Annals of Animal Science   15 ( 2 )   349 - 358   2015.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1515/aoas-2015-0014

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  • Structure and Polymorphism of the Major Histocompatibility Complex Class II Region in the Japanese Crested Ibis, Nipponia nippon

    Yukio Taniguchi, Keisuke Matsumoto, Hirokazu Matsuda, Takahisa Yamada, Toshie Sugiyama, Kosuke Homma, Yoshinori Kaneko, Satoshi Yamagishi, Hiroaki Iwaisaki

    PLOS ONE   9 ( 9 )   2014.9

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    DOI： 10.1371/journal.pone.0108506

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  • Use of In Ovo Chorioallantoic Membrane Engraftment to Culture Testes from Neonatal Mice

    Emi Uematsu, Sachio Takino, Hidemi Okajima, Bin Tong, Toshie Sugiyama, Takahisa Yamada, Sueo Niimura, Hideaki Yamashiro

    COMPARATIVE MEDICINE   64 ( 4 )   264 - 269   2014.8

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  • Identification of novel genetic markers and evaluation of genetic structure in a population of Japanese crested ibis.

    Kanako Tsubono, Yukio Taniguchi, Hirokazu Matsuda, Takahisa Yamada, Toshie Sugiyama, Kosuke Homma, Yoshinori Kaneko, Satoshi Yamagishi, Hiroaki Iwaisaki

    ANIMAL SCIENCE JOURNAL   85 ( 4 )   356 - 364   2014.4

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    DOI： 10.1111/asj.12155

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  • Polymorphisms of the dopamine receptor D4 gene in the Sado population of the Japanese crested ibis Reviewed

    Mai YAMASHITA, Yuuta KOMATSU, Takahisa YAMADA, Bin TONG, Takeshi OHTA, Toshie SUGIYAMA, Hideaki YAMASHIRO, Yoshinori KANEKO, Hisashi NAGATA, Hiroaki IWAISAKI

    The Journal of Animal Genetics   42 ( 2 )   65 - 69   2014

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Breeding and Genetics  

    DOI： 10.5924/abgri.42.65

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  • The nucleotide sequences for the dopamine receptor D4 gene in the Japanese crested ibis Reviewed

    The Journal of Animal Genetics   42 ( 1 )   3 - 9   2014

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    Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Breeding and Genetics  

    DOI： 10.5924/abgri.42.3

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  • Genome-Wide SNP and STR Discovery in the Japanese Crested Ibis and Genetic Diversity among Founders of the Japanese Population

    Yukio Taniguchi, Hirokazu Matsuda, Takahisa Yamada, Toshie Sugiyama, Kosuke Homma, Yoshinori Kaneko, Satoshi Yamagishi, Hiroaki Iwaisaki

    PLOS ONE   8 ( 8 )   2013.8

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    DOI： 10.1371/journal.pone.0072781

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  • Genetic Diversity and Structure in the Sado Captive Populationof the Japanese Crested Ibis

    Kensuke Urano, Kanako Tsubono, Yukio Taniguchi, Hirokazu Matsuda, Takahisa Yamada, Toshie Sugiyama, Kosuke Homma, Yoshinori Kaneko, Satoshi Yamagishi, Hiroaki Iwaisaki

    ZOOLOGICAL SCIENCE   30 ( 6 )   432 - 438   2013.6

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    DOI： 10.2108/zsj.30.432

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  • Effects of 25-hydroxy-cholecalciferol on the development of osteochondrosis in swine

    Toshie Sugiyama, Seiji Kusuhara, Thau Kiong Chung, Hiroshi Yonekura, Elisabeth Azem, Takehiko Hayakawa

    ANIMAL SCIENCE JOURNAL   84 ( 4 )   341 - 349   2013

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    DOI： 10.1111/asj.12000

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  • High ambient temperature influences eggshell quality and calbindin-D28k localization of eggshell gland and all intestinal segments of laying hens. International journal

    T A Ebeid, T Suzuki, T Sugiyama

    Poultry science   91 ( 9 )   2282 - 7   2012.9

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    Eggshell quality deterioration associated with heat stress is a well-known phenomenon. The involvement of the 28-kDa calcium-binding protein (calbindin, CaBP-D28k) localization in this failure is not clearly understood. To test a possible direct effect of ambient temperature on calbindin-D28k localization, 40 White Leghorn laying hens were housed in individual cages and exposed to high ambient temperature (30-33°C) and thermoneutral temperature (20-22°C) which served as a control. Eggshell quality characteristics and immunohistochemical localization of all intestinal segments and eggshell gland calbindin-D28k were performed under both environmental conditions. As expected, egg weight, eggshell thickness, eggshell percentage, and eggshell density were negatively affected by high ambient temperature (P ≤ 0.01). Immunohistochemistry showed that calbindin was localized in the intestinal enterocyte cytoplasm and glandular cell cytoplasm under thermoneutral conditions. However, the calbindin intensity was prominently decreased in ileum, cecum, colon, and eggshell gland under heat stress conditions. Therefore, it could be concluded that calbindin-D28k localization in intestinal segments and eggshell gland is negatively affected by high ambient temperature which might be related to the deterioration of eggshell quality characteristics under heat stress conditions.

    DOI： 10.3382/ps.2011-01898

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  • Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica) Reviewed

    Shinji Hiyama, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida

    JOURNAL OF VETERINARY SCIENCE   13 ( 3 )   223 - 227   2012.9

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    DOI： 10.4142/jvs.2012.13.3.223

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  • Sequential Expression of Osteoblast PhenotypicGenes During Medullary Bone Formation and Resorption in Estrogen-Treated Male Japanese Quails Reviewed

    Shinji Hiyama, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   318B ( 5 )   344 - 352   2012.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jez.b.22451

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  • The W- and Z-linked EE0.6 sequences used for molecular sexing of captive Japanese crested ibis on Sado Island. International journal

    Kaori Kasuga, Maho Higashi, Takahisa Yamada, Toshie Sugiyama, Yukio Taniguchi, Hiroaki Iwaisaki, Kosuke Homma, Yuuichi Wajiki, Yoshinori Kaneko, Satoshi Yamagishi

    Animal science journal = Nihon chikusan Gakkaiho   83 ( 1 )   83 - 7   2012.1

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    The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR-based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W- and Z-linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR-based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR-based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis.

    DOI： 10.1111/j.1740-0929.2011.00971.x

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  • Polymorphism in Promoter Region of CDC10 Gene Showing Marbling-Associated Expression Changes

    Bin Tong, Youji Muramatsu, Takuji Yamamoto, Hideki Tanomura, Takeshi Ohta, Hiroyuki Kose, Toshie Sugiyama, Takahisa Yamada

    JOURNAL OF ANIMAL AND VETERINARY ADVANCES   11 ( 11 )   1930 - 1933   2012

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  • Effects of beta-Cryptoxanthin on Bone Metabolism in a Rat Model of Osteoporosis Reviewed

    Noriko Ikeda, Toshie Sugiyama, Toshiko Suzuki, Katsuyuki Mukai, Seiji Kusuhara

    JOURNAL OF ANIMAL AND VETERINARY ADVANCES   11 ( 1 )   30 - 35   2012

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  • Preliminary Association Study of SNPs in MFN2 Gene Showing Marbling-Associated Expression Changes

    Bin Tong, Youji Muramatsu, Takuji Yamamoto, Hideki Tanomura, Takeshi Ohta, Hiroyuki Kose, Toshie Sugiyama, Takahisa Yamada

    JOURNAL OF ANIMAL AND VETERINARY ADVANCES   11 ( 15 )   2796 - 2800   2012

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  • Useful Marker Around TRDN Gene Showing Marbling-Associated Expression Changes

    Bin Tong, Youji Muramatsu, Takuji Yamamoto, Hideki Tanomura, Takeshi Ohta, Hiroyuki Kose, Toshie Sugiyama, Takahisa Yamada

    JOURNAL OF ANIMAL AND VETERINARY ADVANCES   11 ( 15 )   2792 - 2795   2012

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  • Mechanism of Visceral Fat Reduction in Tsumura Suzuki Obese, Diabetes (TSOD) Mice Orally Administered beta-Cryptoxanthin from Satsuma Mandarin Oranges (Citrus unshiu Marc) International journal

    Katsuhiko Takayanagi, Sho-ichi Morimoto, Yoshiyuki Shirakura, Katsuyuki Mukai, Toshie Sugiyama, Yoshihiko Tokuji, Masao Ohnishi

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   59 ( 23 )   12342 - 12351   2011.12

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    DOI： 10.1021/jf202821u

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  • Non-invasive sampling technique for DNA extraction from captive Japanese Crested Ibis on Sado Island

    Kensuke Urano, Takahisa Yamada, Yukio Taniguchi, Hiroaki Iwaisaki, Toshie Sugiyama, Kosuke Homma, Yoshinori Kaneko, Satoshi Yamagishi

    ANIMAL SCIENCE JOURNAL   82 ( 4 )   616 - 619   2011

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    DOI： 10.1111/j.1740-0929.2011.00902.x

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  • Intensified nursing dramatically accelerates growth performance and the size of the body frame in Japanese black and holstein crossbred steers Reviewed

    Sunao Inada, Fumio Ebara, Sohei Asaoka, Kenichi Asada, Yoshihiro Isozaki, Akira Saito, Toshie Sugiyama, Takafumi Gotoh

    Journal of Animal and Veterinary Advances   9 ( 6 )   1037 - 1047   2010

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    DOI： 10.3923/javaa.2010.1037.1047

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  • Cartilage Pathology of Intra-articular Papain-induced Leg Weakness in a Pig Model

    Sachiko ARAI, Seigo ITOH, Toshie SUGIYAMA, Seiji KUSUHARA, Fujiko SUNAGA

    Nihon Yoton Gakkaishi   47 ( 3 )   136 - 138   2010

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    DOI： 10.5938/youton.47.136

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  • Clinical model of leg weakness induced by intraarticular administration of papain in pigs

    S Arai, S Itoh, M Kachi, T Sugiyama, S Kusuhara

    Japanese Journal of Swine Science   46 ( 4 )   183 - 189   2009.12

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  • トキの頚部筋系の形態学的観察 Reviewed

    金子良則, 遠藤秀紀, 成島悦雄, 橋崎文隆, 杉山稔恵, 楠原征治

    動物園水族館雑誌   50 ( 3-4 )   79 - 87   2009.8

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  • Morphological Observations on the Cervical Vertebrae of a Japanese Crested Ibis, Nipponia nippon

    金子良則, 遠藤秀紀, 成島悦雄, 橋崎文隆, 杉山稔恵, 楠原征治

    動物園水族館雑誌   50 ( 2 )   47 - 55   2009.5

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  • Evidence for the expression of estrogen receptors in osteogenic cells isolated from hen medullary bone Reviewed

    Shinji Hiyama, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida

    ACTA HISTOCHEMICA   111 ( 6 )   501 - 507   2009

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    DOI： 10.1016/j.acthis.2008.06.003

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  • Ultrastructural study of chondrocytes and cartilage canals in tibial dyschondroplasia-induced chicks Reviewed

    The Japanese journal of animal hygiene   33 ( 4 )   143 - 149   2008.3

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  • Endochondral ossification of chick embryonic femora in vitro and on chorioallantoic membrane Reviewed

    Manami Kachi, Toshie Sugiyama, Seiji Kusuhara

    Journal of Poultry Science   45 ( 1 )   51 - 56   2008

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    DOI： 10.2141/jpsa.45.51

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  • Morphological observation of induced lesions of tibial dyschondroplasia in broiler chicken Reviewed

    The Japanese journal of animal hygiene   33 ( 3 )   71 - 78   2007.11

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  • Changes in localization of lysosomal enzymes in muscle induced by conditioning or high pressure treatment. Reviewed

    Fukumura, M, Sugiyama, T, Nishiumi, T, Suzuki, A

    High Pressure Bioscience and Biotechnology   1   239 - 244   2007.8

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  • Expression and localisation of calbindin D28k in all intestinal segments of the laying hen. International journal

    T Sugiyama, H Kikuchi, S Hiyama, K Nishizawa, S Kusuhara

    British poultry science   48 ( 2 )   233 - 8   2007.4

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    1. The aim of the present study was to investigate expression and localisation of a 28-kDa calcium-binding protein (CaBP-D28k) related to active calcium (Ca) absorption, in the entire intestine of egg-laying hens. 2. Western blotting analysis showed that the entire intestine expressed CaBP-D28k to the following degree: duodenum > jejunum > caecum > ileum > colon. Immunohistochemistry showed strong CaBP-D28k localisation in enterocytes along the villus tip-crypt axis in the duodenum and in villus tips in the caecum and colon. The jejunum and ileum had moderate localisation with respect to the number of immunoreactive cells and staining intensity. 3. These results suggest that laying hens actively absorb Ca in both the large and small intestines.

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  • Changes in the Outer Shape, Inner Structure and Bone Mineral Density of the Tibia in Growing Japanese Quail, Coturnix japonica

    Kouichi Nishimura, Susumu Miyamoto, Takao Takeda, Eishi Ayukawa, Toshie Sugiyama, Seiji Kusuhara

    Journal of Poultry Science   44 ( 4 )   426 - 432   2007

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  • Changes in the immunogold electron-microscopic localization of calpain in bovine skeletal muscle induced by conditioning and high-pressure treatment Reviewed

    Gerelt Borjigin, Shuhei Yamamoto, Toshie Sugiyama, Tadayuki Nishiumi, Atsushi Suzuki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   70 ( 5 )   1249 - 1254   2006.5

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    DOI： 10.1271/bbb.70.1249

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  • Expression and localization of estrogen receptors α and β mRNA in medullary bone of laying hens Reviewed

    Tomohiko Imamura, Toshie Sugiyama, Seiji Kusuhara

    Animal Science Journal   77 ( 2 )   223 - 229   2006.4

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    DOI： 10.1111/j.1740-0929.2006.00341.x

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  • Cellular and molecular phenotypes of osteogenic cells isolated from the medullary bone of the hen in vitro Reviewed

    Shinji Hiyama, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida

    Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   142 ( 4 )   419 - 425   2005.12

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    DOI： 10.1016/j.cbpb.2005.09.006

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  • Medullary Bone Formation and Resorption in Avian Calcium Metabolism

    SUGIYAMA Toshie

    Japanese journal of poultry science   42 ( 4 )   J197 - J208   2005.11

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  • Effects of Calcium Regulating Hormones on Osteoclast-Like Cell Formation in Hen Medullary Bone Marrow Culture

    Toshie Sugiyama, Midori Sakurai, Shinji Hiyama, Seiji Kusuhara

    Journal of Poultry Science   41 ( 4 )   307 - 314   2004.10

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    DOI： 10.2141/jpsa.41.307

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  • The Japanese journal of animal hygiene

    28 ( 2 )   65 - 70   2002.12

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  • Osteoclast-like cell formation in medullary bone marrow cell culture of laying hens "jointly worked"

    SUGIYAMA Toshie, SAKURAI Midori, HIYAMA Shinji, KUSUHARA Seiji

    Journal of Poultry Science   39 ( 4 )   256 - 265   2002.10

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  • Changes in immunoelectron microscopic localization of cathepsin D in muscle induced by conditioning or high-pressure treatment

    T Kubo, B Gerelt, GD Han, T Sugiyama, T Nishiumi, A Suzuki

    MEAT SCIENCE   61 ( 4 )   415 - 418   2002.8

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    DOI： 10.1016/S0309-1740(01)00214-5

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  • Morphological comparison of subcultured Typanosoma theileri from grazing cattle

    The Japanese journal of animal hygiene   27 ( 2 )   65 - 70   2002.3

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  • Immunohistochemical study of matrix metalloproteinase-3 (MMP-3) at the articular cartilage in oseteochondrotic pigs "jointly worked"

    Animal Science Journal   73, 517-522   2002

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  • Avian calcium metabolism and bone function Invited Reviewed

    T Sugiyama, S Kusuhara

    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES   14   82 - 90   2001.12

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  • Pressure-induced changes in the connectin/titin localization in the myofibrils revealed by immunoelectron microscopy

    A Suzuki, Y Homma, K Kim, Y Ikeuchi, T Sugiyama, M Saito

    MEAT SCIENCE   59 ( 2 )   193 - 197   2001.10

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    DOI： 10.1016/S0309-1740(01)00070-5

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  • Model selection and genetic parameter estimation for performance traits, body measurement traits and leg seore traits in a closed population of Duroc pigs

    K. Fukawa, T. Sugiyama, S. Kusuhara, O. Kudoh, K. Kameyama

    Animal Science Journal   72 ( 2 )   97 - 106   2001.3

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  • Egg-Laying and calcium Metabolism of Domestic Hens

    T. Sugiyama

    Hokushinetsu Journal of Animal Science   83   17 - 24   2001

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  • Estimation of Genetic Parameters on Leg Score and Joint Cartilage Lesion Scores in a Closed Population of Duroc Pig "jointly worked"

    FUKAWA Kazuo, SUGIYAMA Toshie, KUSUHARA Seiji, KUDOH Osamu, KAMEYAMA Kenji

    Animal Science Journal   71 ( 4 )   353 - 362   2000.7

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    Closed population of 6 generations Duroc pigs was selected for higher productivity and better leg soundness. The leg joint lesions of 174 slaughtered pigs at approximately 90kg live weight were scored from 0 (no lesion) to 4 (very severe lesion) in optical, radiographical and histological standpoints throughout the six generations. Moreover, one thousand and three hundred eighty three pigs were scored from 1 (very good) to 5 (very weak) for leg weakness. Significant differences in the leg weakness score and joint lesion scores were observed among selected generations. Significant differences were observed between gilt and boar/barrow group in 9 traits. The heritabilities of joint lesion scores were low through intermediate. The heritabilities of histological joint scores were also low through intermediate. The phenotypic and genetic correlations between the scores of front (humerus and ulna) and rear legs (femur) were low. The genetic correlations between optical, radiographical and histological scores of the same joint were generally high. The phenotypic correlations between the leg weakness score and joint lesion scores were positive although statistically not significant from zero. The genetic correlations between the leg weakness score and joint lesion scores were generally intermediately or highly positive. The result suggested that selection based on joint lesion scores could lead to a better leg joint condition optically and histologically. It also suggested that selection based on leg weakness score could lead to the reduction of leg joint lesion according to the correlated response.

    DOI： 10.2508/chikusan.71.353

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  • Perspectives on osteoblast and osteoclast function

    CV Gay, VR Gilman, T Sugiyama

    POULTRY SCIENCE   79 ( 7 )   1005 - 1008   2000.7

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  • Effects of the different dose of estrogen and androgen on the development of chicken medullary bone

    T.Sugiyama, N. Nunomura, S. Kusuhara, N. Tsushima

    Hokushinetsu Journal of Animal Science   79   42 - 45   1999

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  • Characteristic of the Bone Matrix Formed by Osteogenic Cells Isolated from Embryonic Chick Calvaria and Hen Medullary Bones "jointly worked"

    Shinji HIYAMA, Toshie SUGIYAMA, Seiji KUSUHARA

    35 ( 4 )   228 - 233   1998.7

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    The characteristics of the bone matrices formed by osteogenic cells isolated from both chick embryonic calvaria and hen medullary bones were examined. Isolated calvarial cells had developed organelles in the cytoplasm compared to isolated medullary bone cells. The cells isolated from both types of bones exhibited alkaline phosphatase (ALP) activity. Isolated calvarial cells formed a matrix that contained small amounts of acid mucopolysaccharide and densely-distributed thick collagen fibers. On the other hand, isolated medullary bone cells formed a matrix that contained a large amount of acid mucopolysaccharide and sparsely-distributed thin collagen fibers. Both matrices displayed calcium deposits. These results suggest that cells isolated from embryonic chick calvaria and hen medullary bones have the capacity for osteogenesis, forming cortical bone-like matrix and medullary bone-like matrix, respectively.

    DOI： 10.2141/jpsa.35.228

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  • Adhesive and Bone Resorptive Activitties of Isolated Osteoclasts from Hen Medullary Bone "jointly worked"

    Toshie SUGIYAMA, Seiji KUSUHARA

    The Journal of Veterinaty Medical Science   60 ( 5 )   573 - 578   1998

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    In the present study, osteoclasts were isolated from hen medullary bones at the formative and resorptive phases. The cells were cultured on glass culture dishes and bone slices. After culturing, the adhesion activity of the isolated osteoclasts with the substrates was estimated with a light microscope, and the surfaces of the bone slices were observed with a scanning electron microscope. The results showed that the adhesion activity of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts is higher at the bone resorptive phase than at the bone formative phase, and this tendency in isolated osteoclasts was observed more frequently on the bone slices than on the glass culture dishes. Furthermore, scanning electron microscopy showed that the isolated osteoclasts in the bone resorptive phase adhered to the bone surface with developed-cytoplasmic projections and formed broad pits where collagen fibrils were exposed. On the other hand, isolated osteoclasts in the bone formative phase adhered to the bone slice with board-shaped cytoplasmic projections and did not form any pits. These results suggest that isolated osteoclasts in the bone resorptive phase have a high level of adhesion activity and actively resorb the bone, whereas isolated osteoclasts in the bone formative phase have a low level of adhesion activity and cease bone resorption. The procedure reported here is useful for studying the bone-resorptive mechanism of authentic osteoclasts.

    DOI： 10.1292/jvms.60.573

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  • Immunohistochemical studies of Ca<sup>2+</sup>-ATPase and carbonic anhydrase II in the shell gland of egg-laying hens

    Tatsuya ARAI, Toshie SUGIYAMA, Seiji KUSUHARA

    Japanese Poultry Science   33 ( 6 )   371 - 376   1996.11

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    The localizations of calcium-adenosine triphosphatase (Ca<sup>2+</sup> -ATPase) and carbonic anhydrase II (CAII) in the shell gland of the hen oviduct were examined by immunohistochemical technique.<br>Immunoreactions showing Ca<sup>2+</sup> -ATPase localization were observed strongly in the luminal surface of the tubular gland cells and in the excretory duct of the tubular gland, but not in the epithelial cells. On the other hand, Immunoreactions showing CAII localization were observed strongly in the cytoplasm of epithelial cells and moderately in the cytoplasm of tubular gland cells. In the shell gland, these immunoreactions showing Ca<sup>2+</sup> -ATPase and CAII localization were not different at between 3 hours and 12 hours after oviposition.<br>These results suggest that Ca<sup>2+</sup> is secreted by active transport of Ca<sup>2+</sup> -ATPase into the shell gland lumen from the tubular gland and HCO<sub>3</sub><sup>-</sup> is produced and secreted in the surface epithelium rather than in the tubular gland.

    DOI： 10.2141/jpsa.33.371

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    Other Link： http://agriknowledge.affrc.go.jp/RN/2010542236

  • Effects of parathyroid hormone and calcitonin on kinetics of actin filaments in chicken osteoclasts of cultured medullary bone

    SUGIYAMA Toshie, KUSUHARA Seiji

    Animal Science and Technology   67 ( 6 )   526 - 532   1996.6

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    Medullary bones dissected from hen femurs at 3 hours (when osteoclasts cease bone resorption) and 12 hours (when osteoclasts actively resorb bone) after oviposition were cultured in BGJb media containing synthetic human parathyroid hormone (PTH) and synthetic eel calcitonin (CT), respectively, and the effects of PTH and. CT on the distributional patterns of actin filaments in the medullary bone osteoclasts were observed. Three hours after oviposition, the osteoclasts displayed strong fluorescence of actin filaments in the form of amorphous bands in the apical region of the cytoplasm, corresponding to clear zones. There was no change in the distributional patterns of actin filaments in osteoclasts after culturing without PTH for 24 hours. However, after culturing with PTH for 24 hours, central parts of the actin bands in the osteoclasts became obscure, and the osteoclasts displayed actin filaments as linear forms perpendicular to the bone surface. On the other hand, 12 hours after oviposition, actin filaments appeared as lines perpen-dicular to the bone surface in the apical region of the cytoplasm, where ruffled borders had developed. There was no change in the distributional patterns of actin filaments in osteoclasts after culturing without CT for 24 hours. However, after culturing with PTH for 24 hours, the linear actin filaments disappeared and the osteoclasts displayed amorphous bands of actin filaments in the apical region of the cytoplasm. These results suggested that PTH and CT would regulate osteoclastic bone resorption by modifying the organization of actin filaments in osteoclasts.

    DOI： 10.2508/chikusan.67.526

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  • Effect of Sex Hormones on Medullary Bone Formation in Cultured Jpapanese Quail Bones

    Kumiko Kato, Toshie Sugiyama, Seiji Kusuhara

    Anim. Sci. Technol. (Jpn.)   67 ( 1 )   64 - 68   1996.1

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  • THE KINETICS OF ACTIN-FILAMENTS IN OSTEOCLASTS ON CHICKEN MEDULLARY BONE DURING THE EGG-LAYING CYCLE

    T SUGIYAMA, S KUSUHARA

    BONE   15 ( 3 )   351 - 353   1994.5

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  • Effect of parathyroid hormone on osteoclasts in organ-cultured medullary bone "jointly worked"

    Toshie Sugiyama, Seiji Kushara

    Japanese Poultry Science   31 ( 6 )   392 - 399   1994.1

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  • ULTRASTRUCTURAL-CHANGES OF OSTEOCLASTS ON HEN MEDULLARY BONE DURING THE EGG-LAYING CYCLE

    T SUGIYAMA, S KUSUHARA

    BRITISH POULTRY SCIENCE   34 ( 3 )   471 - 477   1993.7

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  • Inhibition of osteoclastic bone resorption by calcitonin in the cultured medullary bone of laying hens "jointly worked"

    Toshie SUGIYAMA, Tomoo OHASHI, Seiji KUSUHARA

    Japanese Poultry Science   30 ( 1 )   16 - 23   1993.1

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  • Electron microscopic observations of osteoclasts and osteoblasts in cultured medullary bones of laying hens

    SUGIYAMA Toshie, OHASHI Tomoo, KUSUHARA Seiji

    Animal Science and Technology   65 ( 5 )   468 - 473   1992.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Animal Science  

    Ultrastructural features of osteoclasts and osteoblasts on the medullary bone surface were observed in the cultured bones of laying hens via an electron microscope. The medullary bones were excised from the femur during the formative phase when the eggs were in the magnum of the oviduct and during the resorptive phase when the eggs were in the shell gland of the oviduct. During the formative phase, osteoclasts had abundant cytoplasm but no ruffled border and osteoblasts showed well developed cytoplasm containing a large amount of rough-surfaced endoplasmic reticulum within 48 hours of culturing. These characteristics of osteoclasts and osteoblasts did not differ from those before culturing. During the resorptive phase, on the other hand, osteoclasts had a ruffled border of finger-like cell processes within 72 hours and osteoblasts had poorly developed cytoplasm within 24 hours. These features of osteoclasts and osteoblasts did not differ from those before culturing. These results indicate that the function of osteoclasts and osteoblasts on the medullary bone surface is maintained for a short period of culturing.

    DOI： 10.2508/chikusan.63.468

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Books

  • 家禽の事典

    日本家禽学会

    朝倉書店  2025.6  （ ISBN:9784254450330 ）

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    Total pages：xv, 431p, 図版8p(ページ付なし)   Language：Japanese

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  • 獣医組織学

    日本獣医解剖学会

    学窓社  2023.3  （ ISBN:9784873627878 ）

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    Total pages：439p   Language：Japanese

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  • Sturkie's avian physiology

    Scanes, C. G., Sturkie, Paul D.

    Academic Press  2015  （ ISBN:9780124071605 ）

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    Total pages：xxv, 1028 p.   Language：English

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  • ニワトリの科学 (シリーズ・家畜の科学)

    古瀬 充宏

    朝倉書店  2014.7  （ ISBN:4254455046 ）

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    Total pages：202  

    ASIN

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  • 生物科学・食品科学への高圧利用

    山本 克博, 林 力丸

    さんえい出版  2003.8  （ ISBN:4915592766 ）

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    Total pages：142  

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  • Calcium Metabolism : Comparative Endocrinology

    Bio Scientifica  1999 

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  • The Comparative Endocrinology of Calcium Regulation

    Journal of Endocrinology Limited  1996 

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Awards

  • Prize for emcouragement of Japan Poultry Science Association

    2004  

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    Country：Japan

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Research Projects

  • Elucidating Extracellular Vesicle–Mediated Bone–Muscle Crosstalk Mechanisms for Robust Skeletal Development in Poultry

    Grant number：23K05518

    2023.4 - 2026.3

    System name：Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

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  • Cross-talk mechanism between muscle-derived irisin and bone-derived osteocalcin for robust skeletal constriction in poultry

    Grant number：20K06361

    2020.4 - 2023.3

    System name：Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

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  • Bone-muscle crosstalk for avian bone health

    Grant number：17K08041

    2017.4 - 2020.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    SUGIYAMA Toshie

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    A large amount of economic loss in poultry industry is produced by specific disorders such as leg weakness and eggshell broken. These disorders are caused by the insufficiency of the bone growth and mineralization.
    This research project demonstrated that bone-derived osteokine osteocalcin was highly localized and expressed in the sternum of broiler embryo as compared with layer embryo. It was also suggested that the expression of irisin as muscle-derived myokine was highest in pectoralis of broiler embryo. These results indicate the bone growth may be closely related with muscle-derived myokines, and conversely the muscle growth may be closely related with bone-derived osteokine. Therefore, the bone-muscle crosstalk should contribute to improve the bone growth and mineralization.

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  • Phosphorus Metabolism in Poultry: Prevention of Eggshell Broken and Leg Weakness

    Grant number：26450374

    2014.4 - 2017.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    SUGIYAMA Toshie

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    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    A large amount of economic loss in poultry industry is produced by specific disorders such as the leg weakness and eggshell broken. The disorders are closely related with bone metabolism. Bone is composed of hydroxyapatite (phosphorus and calcium). Therefore, phosphorus metabolism also plays an important role in bone health.
    In this research project, the mRNAs of sodium-dependent phosphate transporter (NaPi-II) were expressed in intestines and kidney, and the NaPi-II mRNAs were increased with the growth and maturation of female chickens. The NaPi-II mRNA expression was also stimulated by 1,25-hydroxyvitamin D3 (1,25-OH2D3) and estrogen. These results demonstrated that phosphorus metabolism during sexual maturation is regulated by the estrogen-induced 1,25-OH2D3 production.

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  • The mechanism of synchronization among the shell gland and medullary bone on the eggshell calcification in Japanese quail.

    Grant number：24659813

    2012.4 - 2015.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Challenging Exploratory Research

    Awarding organization：Japan Society for the Promotion of Science

    YAMAMOTO Toshio, IKEGAME Mika, KAWAI Mariko, SUGIYAMA Toshie

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    To study the synchronizing mechanism among shell gland and medullary bone for the eggshell calcification in Japanese quail, the effects of progesterone and the mechanical tension caused by plumped shell gland itself by an egg over the osteoclastic activity of medullary bone were examined. Progesterone administered at the active stage of medullary bone osteoclasts inhibited the osteoclasts. And the extraction of an egg from shell gland to remove mechanical tension also caused the inhibition of osteoclastic activity in medullary bone. These results strongly suggest that progesterone, and the mechanical tension in shell gland by pumping, respectively may play important roles for the periodic change of the osteoclastic activity synchronized with egg laying cycle in medullary bone.

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  • Novel Metabolism of Vitamin D in Poultry: Prevention of the Leg Weakness and Eggshell Broken

    Grant number：23580386

    2011 - 2013

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    SUGIYAMA Toshie, YAMADA Takahisa

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    Grant amount：\5330000 （ Direct Cost: \4100000 、 Indirect Cost：\1230000 ）

    A large amount of economic loss is produced by several disorders related with calcium metabolism, the leg weakness and eggshell thinning, in the poultry industry. These disorders have been considered as a consequence of the disruption of vitamin D metabolism. In general, the biologically active form (1a,25-(OH)2D3) is converted from vitamin D3 via a two-step hydroxylation process catalyzed by 25-hydroxylase in the liver and 1a-hydroxylase in the kidney.
    In this research project, the mRNA expression of 1a-hydroxylase was indicated in the extra-renal vitamin D-target tissues such as intestines and eggshell gland. The expression was also stimulated by estrogen, and inhibited with aging. These results suggested that 1a,25-(OH)2D3 is locally synthesized from 25-hydroxycholecalciferol (25-(OH)D3) in target-tissues, and directly regulates calcium metabolism. Here, we furthermore suggested that 25-(OH)D3 supplement improves eggshell quality by modification of local vitamin D metabolism.

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  • HIGH PRESSURE REGULATION OF MUSCLE PROTEIN

    Grant number：15208025

    2003 - 2005

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    SUZUKI Atsushi, NISHIUMI Tadayuki, HARA Takashi, SUGIYAMA Toshie, IKEUCHI Yoshihide

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    Grant amount：\27040000 （ Direct Cost: \20800000 、 Indirect Cost：\6240000 ）

    The regulation of muscle protein by high pressure was investigated to elucidate the mechanism of pressure-induced meat tenderization and acceleration of meat conditioning focussing on the endogenous protease in the muscle. The elimination of meat allergenicity by high pressure and pressure effects on the enzymes producing taste-related components in the muscle were also investigated.
    1) Effect on tropomyosin and α-actinin
    (1)α-helix content of α-actinin decreased from 60% to 40% with the increase of pressure applied, while tropomyosin was resistive to high pressure.
    (2)Structural changes of α-actinin by high pressure seem to be reversible under 300 MPa and not reversible over 300 MPa.
    (3)Superprecipitation(SPPT)-inducing activity of α-actinin highly correlates with actin gelation activity, rather than that with ATPase acceleration.
    2)Combined effects of high pressure and heat
    (1)The combination of high pressure and heat treatments seem to be effective to tenderize tough meat.
    (2)The shear force value may have some relationship with deformation of intramuscular connective tissue and myofibrils.
    3)Effect on intramuscular connective tissue
    (1)Not only muscle fibrils but also connective tissue was tenderized by high-pressure treatment.
    (2)Pressure-dependent decrease of thermal stability and structural weakening of intramuscular collagen was observed.
    4)Effect on endogenous proteases in muscle
    (1)High pressure led to proteasome (one of endogenous proteases in the muscle) activation and irreversible change in the tertiary structure as detected from fluorescent measurement, whereas the change in the secondary structure was slight compared with that by heat treatment. Therefore pressure-induced activation of the proteasome seems to have been due to a small amount of unfolding of the active cites of proteasome.
    (2)Changes in the localization of calpain (one of endogenous proteases in the muscle) in conditioned and pressurized bovine skeletal muscles were investigated by immunogold electron microscopy. It was clear that pressure-induced changes in calpain localization were drastic in comparison with those during conditioning.
    5)Effect on allergenicity of bovine serum albumin (BSA)
    (1)The effect of high pressure treatment on the elimination of BSA was evaluated on the basis of histamine release from human basophilic KU812 F cells sensitized with sera from allergic patients, and the structural changes of BSA responsible for reducing allergenicity was investigated.
    (2)The reducing effect on the allergenicity gradually increased with the increase of pressure to BSA.
    (3)The pressure-induced elimination of BSA allergenicity seems to be related to the tertiary structural change of BSA.
    6)Effect on the enzymes producing taste-related components in the muscle
    Inosinic acid (IMP; one of the umami components) was produced in meat even by high pressurization, because the enzymatic properties of the enzymes producing taste-related components in the muscle were retained in pressurized meat.

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  • The sensitivity to endocrine disruptors and the establishment of sensitive bioassay for endocrine disruptors in poultry

    Grant number：15580232

    2003 - 2005

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    SUGIYAMA Toshie, KUSUHARA Seiji, NISHIMURA Kouichi

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    Grant amount：\3600000 （ Direct Cost: \3600000 ）

    1.Aromatic hydrocarbon receptor (AhR ; dioxin receptor) mRNA expression with aging.
    We detected the expression of AhR mRNA in various tissues of female chickens with aging (0 to 390 days old) by RT-PCR. As a result, AhR mRNA was detected in gizzard, liver, lung, kidney and heart during all stages. However, the oviduct and bone marrow of female chickens represented no AhR mRNA at 0-day stage and less at 30-90-day stages, respectively. These results suggest that the sensitivities of oviduct and bone marrow to dioxin are altered according to the age of female chickens.
    2.Expression and localization of AhR in oviduct of laying chickens.
    AhR expression was detected in magnum, isthmus and shell gland of oviducts by western blotting analysis. Especially in isthmus and shell gland, AhR was strongly localized in the cytoplasm of their epithelial cells. These results suggest that dioxin directly disrupts the metabolism of isthmus and shell gland, resulting in the eggshell weakness.
    3.In Vivo bioassay of endocrine disruptors using avian medullary bone.
    The treatment of male Japanese quail with DDE (10mg/day) for 7 days induced medullary bone formation in marrow cavities of femurs. Concomitantly, the treatment increased the serum vitellogenin levels. These results suggest that avian medullary bone formation is superior as in vivo bioassay model of endocrine disruptors.
    4.Expression and localization of endocrine disrupter receptors in avian medullary bone.
    Estrogen receptor (α and β) and androgen receptor are well known as receptors for endocrine disruptor. In the present study by in situ hybridization and immunohistochemical analyses, both receptors (especially α subtype in estrogen receptor) were localized in osteoblasts of chicken medullary bone. These results suggest that endocrine disruptors directly act on osteoblastic bone formation.
    5.RT-PCR analysis of vitellogenin mRNA expression in avian liver.
    We tried to detect vitellogenin mRNA expression in liver of male quails treated with estrogen receptor agonists. As a result, vitellogenin mRNA expression was induced by estrogen receptor α agonist but not estrogen receptor β agonist. This indicates that vitellogenin synthesis in liver is stimulated through estrogen receptor α.

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  • Molecular Analysis and Expression Study of the Calcitonin Receptor Gene in Chickens

    Grant number：12760185

    2000 - 2001

    Awarding organization：Japan Society for the Promotion of Science

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    Grant amount：\2300000 （ Direct Cost: \2300000 ）

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  • Studies on the denaturation of muscle proteins under high pressure.

    Grant number：10460118

    1998 - 2000

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B).

    Awarding organization：Japan Society for the Promotion of Science

    SUZUKI Atsushi, NAKAHARA Masaru, IKEUCHI Yoshihide, SUGIYAMA Toshie

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    Grant amount：\13700000 （ Direct Cost: \13700000 ）

    The denaturation of muscle proteins under high pressure was investigated by histological, biochemical and physicochemical methods.
    Actin
    1) The rate of dissociation of nucleotides from actin molecule, disappearance of the characterized ^1H NMR signal at 2.055 ppm and loss in biochemical activities (DNAasel inhibition capacity, polymerizability) at 300 MPa were almost identical. suggesting that the rapid collapse of steric structure around the upper region called "pointed end" of actin molecule following the dissociation of the bound nucleotide.
    2) The decreases of α-helix content were observed with the pressure applied and the denaturation was irreversible in the actin pressurized at 300 MPa or more.
    Myosin and Tropomyosin
    1) The structural changes in myosin molecule pressurized at 200 MPa or more were irreversible.
    2) Tropomyosin which is resistive to acid and heat was also resistive to high pressure.
    Mitochondria and Connective Tissue
    1) The decrease of Ca^<2+> uptaking ability seemed to be correlated with the decrease of ATPase activity which may be caused by the release of ATPase subunits (α β) from mitochondria by pressure.
    2) The deformation of the honeycomb-like structure of endomysium was accelerated with increase of pressure applied, but the changes in the extractability of PGs were not observed in the pressurized muscle.
    Proteasome
    The high pressure treatments up to 50〜100MPa caused the activation of the proteasome. This activation is probably due to the increase of the interaction of the substrate and active site of the proteasome unfolded by high pressure treatment.
    β-Lactoglobulin (BLg) and High Pressure-Thawing
    1) No difference in the ^1NMR spectrum was observed between pressurized and unpressurized BLg. NMR detection of the H/D exchange of aromatic residues indicated that the conformation at the vicinity of tryptophan residues can be refolded after the release of pressure.
    2) It was cleared that the high pressure-thawing was applicable to meat processing.

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Teaching Experience

  • 科学英語演習

    2023
    Institution name：新潟大学

  • 卒業論文I

    2023
    Institution name：新潟大学

  • 卒業論文II

    2023
    Institution name：新潟大学

  • 資源動物科学概論

    2022
    Institution name：新潟大学

  • 生物資源科学概論

    2022
    Institution name：新潟大学

  • 応用農力

    2022
    Institution name：新潟大学

  • スタディ・スキルズAIIb

    2021
    -
    2023
    Institution name：新潟大学

  • スタディ・スキルズAIb

    2021
    -
    2023
    Institution name：新潟大学

  • スタディ・スキルズAIa

    2021
    -
    2022
    Institution name：新潟大学

  • 地域交流ｻﾃﾗｲﾄ実習

    2021
    -
    2022
    Institution name：新潟大学

  • スタディ・スキルズAIIa

    2021
    -
    2022
    Institution name：新潟大学

  • スタディ・スキルズAIc

    2021
    -
    2022
    Institution name：新潟大学

  • スタディ・スキルズAIIc

    2021
    -
    2022
    Institution name：新潟大学

  • キャリアビジョン研修

    2021
    Institution name：新潟大学

  • 実践型食づくりプロジェクト

    2021
    Institution name：新潟大学

  • 動物生産学実験実習Ⅲ

    2020
    Institution name：新潟大学

  • 動物生産学実験実習Ⅳ

    2020
    Institution name：新潟大学

  • 基礎動植物生産学実験

    2020
    -
    2023
    Institution name：新潟大学

  • 基礎農力

    2020
    -
    2022
    Institution name：新潟大学

  • 応用農力

    2020
    -
    2022
    Institution name：新潟大学

  • 生物資源科学演習Ⅱ

    2020
    Institution name：新潟大学

  • スタディ・スキルズA a

    2020
    Institution name：新潟大学

  • スタディ・スキルズA b

    2020
    Institution name：新潟大学

  • 生物資源科学演習Ⅰ

    2020
    Institution name：新潟大学

  • スタディ・スキルズA c

    2020
    Institution name：新潟大学

  • 動物衛生福祉学

    2019
    Institution name：新潟大学

  • 牧場実習

    2019
    Institution name：新潟大学

  • 地域交流サテライト実習

    2019
    -
    2020
    Institution name：新潟大学

  • 植物生産実地見学

    2019
    Institution name：新潟大学

  • 動物解剖生理学

    2018
    Institution name：新潟大学

  • 生命を知る

    2018
    Institution name：新潟大学

  • 農学入門Ⅱ

    2018
    -
    2022
    Institution name：新潟大学

  • 農学入門Ⅰ

    2018
    -
    2022
    Institution name：新潟大学

  • 動物生産学概論

    2018
    -
    2021
    Institution name：新潟大学

  • 農業生産科学特論Ⅱ

    2018
    Institution name：新潟大学

  • 基礎生物学

    2017
    Institution name：新潟大学

  • トキをシンボルとした自然再生

    2015
    -
    2020
    Institution name：新潟大学

  • スタディ・スキルズA1

    2015
    -
    2016
    Institution name：新潟大学

  • 研究発表

    2015
    Institution name：新潟大学

  • 自然科学総論Ⅳ

    2014
    Institution name：新潟大学

  • 研究発表演習(中間発表)

    2012
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅰ

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学特定研究AⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • 生命・食料科学セミナーAⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • 文献詳読Ⅱ

    2012
    -
    2014
    Institution name：新潟大学

  • 生命・食料科学セミナーAⅡ

    2012
    -
    2014
    Institution name：新潟大学

  • 生命・食料科学特定研究AⅡ

    2012
    -
    2014
    Institution name：新潟大学

  • 動物組織細胞化学論

    2011
    Institution name：新潟大学

  • 動物機能形態論

    2011
    Institution name：新潟大学

  • 実践型食づくりプロジェクト

    2011
    -
    2021
    Institution name：新潟大学

  • 企画提案型食づくりプロジェクト

    2011
    -
    2014
    Institution name：新潟大学

  • 生物資源論

    2009
    -
    2016
    Institution name：新潟大学

  • 動物生産学実験実習Ⅰ

    2008
    Institution name：新潟大学

  • 動物生産学実験実習Ⅱ

    2008
    Institution name：新潟大学

  • 動物生産学演習Ⅰ

    2008
    -
    2021
    Institution name：新潟大学

  • 動物生産学演習Ⅱ

    2008
    -
    2021
    Institution name：新潟大学

  • 動物のバイオサイエンス

    2008
    -
    2018
    Institution name：新潟大学

  • 動物生体機構学

    2007
    -
    2018
    Institution name：新潟大学

  • 動物衛生管理学

    2007
    -
    2018
    Institution name：新潟大学

  • 応用動物科学序説

    2007
    -
    2010
    Institution name：新潟大学

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