Graduate School of Science and Technology Life and Food Sciences Associate Professor
Faculty of Science Department of Science Associate Professor
Updated on 2024/10/07
博士(理学) ( 1994.9 新潟大学 )
Cell Biology
細胞生物学
Life Science / Cell biology
Niigata University Faculty of Science Department of Biology Associate Professor
2004.4
Niigata University Graduate School of Science and Technology Life and Food Sciences Associate Professor
2004.4
Niigata University Faculty of Science Associate Professor (as old post name)
1994.11 - 2004.3
Niigata University Faculty of Science Research Assistant
1991.10 - 1994.10
Niigata University Graduate School, Division of Natural Science
- 1990
Niigata University 理学研究科 生物学
- 1990
Country: Japan
Niigata University Faculty of Science
- 1988
Niigata University Faculty of Science Department of Biology
- 1988
Country: Japan
日本血液学会
1988 - 2021
日本細胞生物学会
CD41+ extracellular vesicles produced by avian thrombocytes contain microRNAs
Kenkichi Sugimoto, Takamasa Nishikawa, Toshie Sugiyama
Genes to Cells 2023.12
Kenkichi Sugimoto, Kayano Toume
Genes to Cells 26 ( 10 ) 757 - 771 2021.7
Analysing the evolutional and functional differentiation of four types of Daphnia magna cryptochrome in Drosophila circadian clock. Reviewed International journal
Yohei Nitta, Sayaka Matsui, Yukine Kato, Yosuke Kaga, Kenkichi Sugimoto, Atsushi Sugie
Scientific reports 9 ( 1 ) 8857 - 8857 2019.6
MicroRNA-342 inhibits tumor growth via targeting chemokine CXCL12 involved in macrophages recruitment/activation. Reviewed International journal
Yijun Tian, Sayaka Matsui, Maki Touma, Qiong Wu, Kenkichi Sugimoto
Genes to cells : devoted to molecular & cellular mechanisms 23 ( 12 ) 1009 - 1022 2018.12
Aquaporin-1, a New Maternally Expressed Gene, Regulates Placental Development in the Mouse. Reviewed International journal
Jing Guo, Hongjuan He, Hui Liu, Qi Liu, Lili Zhang, Boqi Liu, Kenkichi Sugimoto, Qiong Wu
Biology of reproduction 95 ( 2 ) 40 - 40 2016.8
CCL11-induced eosinophils inhibit the formation of blood vessels and cause tumor necrosis. Reviewed International journal
Yanjiang Xing, Yijun Tian, Takamasa Kurosawa, Sayaka Matsui, Maki Touma, Takanori Yanai, Qiong Wu, Kenkichi Sugimoto
Genes to cells : devoted to molecular & cellular mechanisms 21 ( 6 ) 624 - 38 2016.6
The atypical IκB protein IκB(NS) is important for Toll-like receptor-induced interleukin-10 production in B cells. Reviewed International journal
Minami Miura, Naoki Hasegawa, Mitsuo Noguchi, Kenkichi Sugimoto, Maki Touma
Immunology 147 ( 4 ) 453 - 63 2016.4
Inhibition of blood vessel formation in tumors by IL-18-polarized M1 macrophages. Reviewed International journal
Yanjiang Xing, Yijun Tian, Takamasa Kurosawa, Sayaka Matsui, Maki Touma, Qiong Wu, Kenkichi Sugimoto
Genes to cells : devoted to molecular & cellular mechanisms 21 ( 3 ) 287 - 95 2016.3
MiR-1188 at the imprinted Dlk1-Dio3 domain acts as a tumor suppressor in hepatoma cells. Reviewed International journal
Wei Cui, Zhijun Huang, Hongjuan He, Ning Gu, Geng Qin, Jie Lv, Tao Zheng, Kenkichi Sugimoto, Qiong Wu
Molecular biology of the cell 26 ( 8 ) 1416 - 27 2015.4
Establishment of a sticky, large, oval-shaped thrombocyte cell line from tree frog as an ancestor of mammalian megakaryocytes. Reviewed International journal
Kenkichi Sugimoto
SpringerPlus 4 447 - 447 2015
Enhancement of phagocytosis and cytotoxicity in macrophages by tumor-derived IL-18 stimulation. Reviewed International journal
Xu Henan, Naoka Toyota, Xing Yanjiang, Yuuki Fujita, Huang Zhijun, Maki Touma, Wu Qiong, Kenkichi Sugimoto
BMB reports 47 ( 5 ) 286 - 91 2014.5
Role of FGF10 on tumorigenesis by MS-K. Reviewed International journal
Kenkichi Sugimoto, Suzuka Yoshida, Yuka Mashio, Naoka Toyota, Yanjiang Xing, Henan Xu, Yuki Fujita, Zhijun Huang, Maki Touma, Qiong Wu
Genes to cells : devoted to molecular & cellular mechanisms 19 ( 2 ) 112 - 25 2014.2
Dynamic expression pattern of Pde4d and its relationship with CpG methylation in the promoter during mouse embryo development. Reviewed International journal
Zhijun Huang, Zhengbin Han, Wei Cui, Fengwei Zhang, Hongjuan He, Tiebo Zeng, Kenkichi Sugimoto, Qiong Wu
Biochemical and biophysical research communications 441 ( 4 ) 982 - 7 2013.11
Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development. Reviewed International journal
Tiantian Gu, Hongjuan He, Zhengbin Han, Tiebo Zeng, Zhijun Huang, Qi Liu, Ning Gu, Yan Chen, Kenkichi Sugimoto, Huijie Jiang, Qiong Wu
Acta histochemica 114 ( 4 ) 392 - 9 2012.7
Effect of vegf gene knockdown on growth of the murine sarcoma cell line MS-K. Reviewed International journal
Xiu Y Zhong, Asami Yoshioka, Yuka Mashio, Toru Ikeda, Huijie Jiang, Maki Touma, Qiong Wu, ChangLiu Wang, Kenkichi Sugimoto
Genes to cells : devoted to molecular & cellular mechanisms 16 ( 6 ) 625 - 38 2011.6
Expression of non-coding RNA AB063319 derived from Rian gene during mouse development. Reviewed International journal
Tiantian Gu, Hongjuan He, Yanjiang Xing, Qi Liu, Ning Gu, Sugimoto Kenkichi, Huijie Jiang, Qiong Wu
Journal of molecular histology 42 ( 2 ) 105 - 12 2011.4
Cold stress and light signals induce the expression of cold-inducible RNA binding protein (cirp) in the brain and eye of the Japanese treefrog (Hyla japonica). Reviewed International journal
Kenkichi Sugimoto, Huijie Jiang
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 151 ( 4 ) 628 - 36 2008.12
HB-1, an acute myeloid leukemic cell line with the capability of infiltrating into the brain in CBA/N mice. Reviewed International journal
Hui-Jie Jiang, Hu-Shan Sun, Xu-Dong Wang, Chang-Liu Wang, Ze-Long Liu, Hiroyuki Gonda, Kenkichi Sugimoto
Sheng li xue bao : [Acta physiologica Sinica] 58 ( 4 ) 377 - 83 2006.8
Murine serum obtained from bone marrow-transplanted mice promotes the proliferation of hematopoietic stem cells by co-culture with MS-5 murine stromal cells. Reviewed International journal
Akemi Nakayama, Haruna Matsui, Teruaki Fukushima, Hiroshi Ichikawa, Kensuke Yamada, Takuji Amao, Masamichi Hosono, Kenkichi Sugimoto
Growth factors (Chur, Switzerland) 24 ( 1 ) 55 - 65 2006.3
Cloning of hibernation-related genes of bullfrog (Rana catesbeiana) by cDNA subtraction. Reviewed International journal
Qiong Wu, Kenkichi Sugimoto, Keiko Moriyama, Yasuhiro Adachi, Akemi Nakayama, Kazuhiro J Mori
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 133 ( 1 ) 85 - 94 2002.9
Identification of negative regulator of interleukin-3 (NIL-3) in bone marrow. Reviewed
Adachi Yasuhiro, Kenkichi Sugimoto, Asako K Sato, Kazuhiro John Mori
Cell structure and function 27 ( 2 ) 81 - 9 2002.4
Induction of the expression of SCF in mouse by lethal irradiation. Reviewed International journal
K Sugimoto, Y Adachi, K Moriyama, W Qiong, A Nakayama, M Hosono, K J Mori
Growth factors (Chur, Switzerland) 19 ( 4 ) 219 - 31 2001
Cloning and characterization of amphibian cold inducible RNA-binding protein Reviewed
Toshifumi Saito, Kenkichi Sugimoto, Yasuhiro Adachi, Qiong Wu, Kazuhiro J. Mori
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 125 ( 2 ) 237 - 245 2000
Mutual education between hematopoietic cells and bone marrow stromal cells through direct cell-to-cell contact: factors that determine the growth of bone marrow stroma-dependent leukemic (HB-1) cells. Reviewed International journal
H Jiang, K Sugimoto, H Sawada, E Takashita, M Tohma, H Gonda, K J Mori
Blood 92 ( 3 ) 834 - 41 1998.8
Establishment and characterization of bone marrow stroma-dependent leukemia cell line, HB-1. Reviewed
H Sawada, K Sugimoto, H Jiang, Y Tsurumaki, G Breckon, S A Lorimore, E G Wright, K J Mori
International journal of hematology 67 ( 3 ) 295 - 306 1998.4
Gene expression of lipid binding protein transferred the ability of specific attachment of hemopoietic cells to non-supportive stromal cell line, MS-K. Reviewed
E Takashita, K Sugimoto, H Jiang, M Tohma, E Urano, H Yokoyama, K J Mori
Cell structure and function 22 ( 6 ) 595 - 602 1997.12
Destruction of hematopoietic microenvironment by cytotoxic T cells. Reviewed International journal
E Takashita, K Sugimoto, Y Adachi, Y Aihara, H Inoue, H Jiang, T Katakai, K J Mori
Experimental hematology 25 ( 10 ) 1034 - 41 1997.9
Strain specific production of a negative regulator of IL-3 (NIL-3): difference in the negative feedback mechanism of hemopoiesis among mouse strains. Reviewed
K Sugimoto, H Jiang, E Takashita, H Inoue, S Kadowaki, K J Mori
Cell structure and function 22 ( 4 ) 407 - 11 1997.8
Cascade regulation of cytokine production in granulopoiesis Reviewed
Kenkichi Sugimoto, Kazuhiro J. Mori
Leukemia 11 ( 3 ) 464 - 467 1997
Implications for the assay and biological properties of interleukin-3. Results of a WHO international collaborative study Reviewed
Anthony R. Mire-Sluis, Rose Gaines Das, Robin Thorpe, Sandra Thomas, Darryl Maher, Peter Valent, Elisabeth Payer, De Groote, Kent Hayglass, H. Ziltener, Lars K. Poulsen, Pierre Miossec, P. Chomarat, Jacques Grassi, G. Pawelec, U. Fritzsche, M. Falk, M. Topp, G. Reisbach, John Sidiropoulos, S. Z. Ben-Sasson, Zeev Even-Chen, Luigi Pegoraro, Francesco Barboni, Kenkichi Sugimoto, K. Tsuji, Tomoaki Matsumoto, Kenrichi Arai, Takashi Yokota, J. Olobo, G. Wagemaker, T. Van Der Pouw-Kraan, Kirrikant V. Sheth, C. Gutierrez, D. Rivas, Nils Lyeke, Karin Schön, John P.A. Bews, K. Tullberg, R. Klotzbücher, L. Page, C. Bird, Colin P. McGuckin, John Gordon, Anita Katira, Peter Openshaw, Andrew Georgiou, Nydia G. Testa, Robin Callard, K. Paik, John McKearn, Marie Rock, Kathleen Harper, Steven J. Swanson, Jay P. Siegel, H. L. Spiegelberg, John Geigert, Kerri Carter, William E. Paul, Cynthia Watson, Monica Tsang, Li Zhou, Louis Westreich, Chun C. Chao, Terry L. Riss, Rich Moravec
Journal of Immunological Methods 194 ( 1 ) 1 - 12 1996.7
Implications for the assay and biological activity of interleukin-4. Results of a WHO international collaborative study Reviewed
Anthony R. Mire-Sluis, Rose Gaines Das, Robin Thorpe, Sandra Thomas, Darryl Maher, Peter Valent, Elisabeth Payer, De Groote, Kent Hayglass, H. Ziltener, Lars K. Poulsen, Pierre Miossec, P. Chomarat, Jacques Grassi, G. Pawelec, U. Fritzsche, M. Falk, M. Topp, G. Reisbach, John Sidiropoulos, S. Z. Ben-Sasson, Zeev Even-Chen, Luigi Pegoraro, Francesco Barboni, Kenkichi Sugimoto, K. Tsuji, Tomoaki Matsumoto, Kenrichi Arai, Takashi Yokota, J. Olobo, G. Wagemaker, T. Van Der Pouw-Kraan, Kirrikant V. Sheth, C. Gutierrez, D. Rivas, Nils Lyeke, Karin Schön, John P.A. Bews, K. Tullberg, R. Klotzbücher, L. Page, C. Bird, Colin P. McGuckin, John Gordon, Anita Katira, Peter Openshaw, Andrew Georgiou, Nydia G. Testa, Robin Callard, K. Paik, John McKearn, Marie Rock, Kathleen Harper, Steven J. Swanson, Jay P. Siegel, H. L. Spiegelberg, John Geigert, Kerri Carter, William E. Paul, Cynthia Watson, Monica Tsang, Li Zhou, Louis Westreich, Chun C. Chao, Terry L. Riss, Rich Moravec
Journal of Immunological Methods 194 ( 1 ) 13 - 25 1996.7
Separate control of the survival, the self-renewal and the differentiation of hemopoietic stem cells. Reviewed
T Yanai, K Sugimoto, E Takashita, Y Aihara, Y Tsurumaki, T Tsuji, K J Mori
Cell structure and function 20 ( 2 ) 117 - 24 1995.4
Induction of bcl-2 gene expression by intercellular information from hemopoietic supportive stromal cells to DA-1 cells. Reviewed International journal
E Takashita, K Sugimoto, Y Adachi, K J Mori
Journal of cellular physiology 161 ( 2 ) 367 - 73 1994.11
Effects of anti-CD44 monoclonal antibody on adhesion of erythroid leukemic cells (ELM-I-1) to hematopoietic supportive cells (MS-5): CD44, but not hyaluronate-mediated, cell-cell adhesion. Reviewed International journal
K Sugimoto, Y Tsurumaki, H Hoshi, S Kadowaki, M C LeBousse-Kerdiles, F Smadja-Joffe, K J Mori
Experimental hematology 22 ( 6 ) 488 - 94 1994.6
Age-related decreases in the reconstituting ability of hemopoietic cells and the ability of hemopoietic microenvironment to support hemopoietic reconstitution in senescence accelerated (SAM-P) mice. Reviewed International journal
A Sakuma, I Tsuboi, K Morimoto, T Horie, K Sugimoto, K J Mori
Mechanisms of ageing and development 73 ( 2 ) 127 - 35 1994.2
Constitutive production of IL-6 in the anemic mice of W/Wv genotype. Reviewed International journal
T Yanai, K Sugimoto, T Tsuji, E Takashita, K J Mori
Leukemia research 18 ( 2 ) 123 - 31 1994.2
INDUCTION OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) AND GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF) EXPRESSION IN BONE-MARROW AND FRACTIONATED MARROW CELL-POPULATIONS BY INTERLEUKIN-3 (IL-3) - IL-3-MEDIATED POSITIVE FEEDBACK MECHANISMS OF GRANULOPOIESIS Reviewed
T TSUJI, K SUGIMOTO, T YANAI, E TAKASHITA, KJ MORI
GROWTH FACTORS 11 ( 1 ) 71 - 79 1994
Colony promoting activity (CPA) is a novel factor distinct from IL-6. Reviewed International journal
K Sugimoto, S Taniguchi, T Yanai, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 47 ( 2-3 ) 67 - 72 1993
Application of poly(L-lactic acid) particles for the suppression of genetic resistance to bone marrow allografts by reticuloendothelial system-blockade. Reviewed International journal
S Kadowaki, K Sugimoto, Y Tsurumaki, Y Tabata, Y Ikada, J Fujita, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 47 ( 9 ) 385 - 91 1993
Production of a low molecular weight growth inhibitory factor by adenovirus 12-transformed cells. Reviewed International journal
T Tsuji, K Mori, K Sugimoto, C Hamada, K J Mori
Biochemical and biophysical research communications 187 ( 3 ) 1232 - 40 1992.9
Production of a negative regulator of IL-3 by bone marrow cells in response to the supernatant of IL-3-producing STIL-3 leukemia cells. Reviewed International journal
K Sugimoto, S Taniguchi, H Ogata, S Ikehara, J Fujita, K J Mori
Leukemia research 16 ( 9 ) 889 - 97 1992.9
Characterization of murine hemopoietic-supportive (MS-1 and MS-5) and non-supportive (MS-K) cell lines. Reviewed International journal
J Suzuki, J Fujita, S Taniguchi, K Sugimoto, K J Mori
Leukemia 6 ( 5 ) 452 - 8 1992.5
Adhesion of mouse mast cells to fibroblasts: adverse effects of steel (Sl) mutation. Reviewed International journal
Y Kaneko, J Takenawa, O Yoshida, K Fujita, K Sugimoto, H Nakayama, J Fujita
Journal of cellular physiology 147 ( 2 ) 224 - 30 1991.5
Long-term cultivation of an interleukin-3-producing cell line in a chemically defined protein-free medium. Reviewed
K Itoh, K Sugimoto, H Sawada, T Uchiyama, H Uchino, K J Mori
International journal of hematology 54 ( 2 ) 171 - 4 1991.4
Prevention of genetic resistance to bone marrow allografts by reticuloendothelial system blockade. Reviewed International journal
S Kadowaki, S Taniguchi, J Suzuki, K Sugimoto, J Fujita, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 45 ( 9 ) 417 - 22 1991
Hierarchical regulation of interleukin production: induction of interleukin 6 (IL-6) production from bone marrow cells and marrow stromal cells by interleukin 3 (IL-3). Reviewed International journal
M Miyashita, K Sugimoto, J Suzuki, S Taniguchi, K Aramaki, K J Mori
Leukemia research 15 ( 12 ) 1125 - 31 1991
Establishment of a sarcoma cell line, MS-K, expressing Ki-ras proto-oncogene product from mouse bone marrow stromal cells. Reviewed International journal
K Shirata, T Suzuki, N Yanaihara, K Sugimoto, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 45 ( 1 ) 1 - 8 1991
Age-related decrease in the number of hemopoietic stem cells and progenitors in senescence accelerated mice. Reviewed International journal
H Izumi-Hisha, Y Ito, K Sugimoto, H Oshima, K J Mori
Mechanisms of ageing and development 56 ( 1 ) 89 - 97 1990.10
Distribution of IL-3 activity in mice transplanted with IL-3 producing T cells (STIL-3): abrogation of the distribution of STIL-3 cells into the liver and liver hemopoiesis by splenectomy. Reviewed International journal
K Aramaki, N Takayama, S Taniguchi, M Miyashita, K Sugimoto, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 44 ( 8 ) 429 - 34 1990
Presence of an activity indispensable for the granulocyte/macrophage colony-stimulating activity of interleukin 3 in bovine serum albumin. Reviewed International journal
K Sugimoto, J Fujita, M Miyashita, K Aramaki, T Tamaki, K J Mori
Leukemia research 14 ( 2 ) 139 - 44 1990
Colony promoting activity: differences from interleukin-3 and similarities to interleukin-6. Reviewed International journal
K Sugimoto, K Shirata, S Kadowaki, I Tsuboi, K Morimoto, S Sawada, K J Mori
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 44 ( 2 ) 135 - 40 1990
Hemopoietic features of splenectomized mice bearing IL-3 producing T cell leukemia. Reviewed International journal
H Sawada, K Sugimoto, K Aramaki, K J Mori
Leukemia research 13 ( 12 ) 1131 - 8 1989
Suppression of mast cell colony formation by a low molecular weight fraction of fetal calf bone marrow extract. Reviewed International journal
M Lenfant, D Sotty, J Wdzieczak-Bakala, K Itoh, K Shirata, K Sugimoto, K J Mori
Leukemia research 13 ( 12 ) 1085 - 9 1989
理系のための基礎生物学
菊山, 宗弘, 酒泉, 満
化学同人 2010.4 ( ISBN:9784759814316 )
骨髄支持細胞による造血幹細胞の分化・増殖制御
別冊医学のあゆみ 血液疾患ver.2 1998
造血細胞と骨髄ストローマ細胞の相互教育
杉本健吉, 高下恵美, 森和博
日本細胞生物学会大会講演要旨集 50th 1997
骨髄間質細胞における造血支持関与遺伝子の検索
高下恵美, 杉本健吉, 森和博
日本細胞生物学会大会講演要旨集 50th 1997
杉本健吉, 森和
生体の科学 47 ( 3 ) 203 - 207 1996.6
森和博, 門脇信悦, 弦巻葉子, 杉本健吉
最新医学 49 ( 3 ) 145(475) - 153(483) 1994.3
老化促進モデル(SAM-P)に於ける幹細胞老化と癌化
森和博, 杉本健吉
18 ( 1 ) 10 - 22 1994
The mutual education between hemopoietic cell and hemopoiesis support intersitial cell.
杉本健吉, 箭内敬典, 高下恵美, 森和博
日本細胞生物学会大会講演要旨集 47th 1994
A role of extracellular matrix in the adhesion of hemopoietic stroma to hemopoiesis supporting cells.
箭内敬典, 杉本健吉, 高下恵美, 朔敬, てい くん, 森和博
日本細胞生物学会大会講演要旨集 47th 1994
骨髄ストローマ細胞と造血因子
辻孝, 杉本健吉, 森和博
Pharma Medica 別冊 11 ( 9 ) 19 - 27 1993
Negative feedback機構としてのNIL-3
杉本健吉
造血因子 (別冊) 4 ( 3 ) 55 - 63 1993
Elucidation of hemostasis control mechanism involving extracellular vesicles released by amphbian thrombocytes
Grant number:18K05552
2018.4 - 2021.3
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
Sugimoto Kenkichi
Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )
Many detailed characteristics of thrombocytes remain unclear. Here, we report the finding that thrombocytes produce integrin alpha IIb (CD41)-positive extracellular vesicles (EVs), which include microRNAs (miRs). FCM analysis revealed the expression of CD41+ on the surface of EVs. Nanotracking analysis demonstrated that these CD41+ EVs were approximately 100 nm in diameter. Microarray analysis revealed that the CD41+ EVs contain many kinds of miRs. These CD41+ EVs were phagocytosed by endothelial cells and macrophages. qPCR analysis revealed that many angiogenesis-related genes were upregulated in CD41+ EV-treated endothelial cells. These results indicated that thrombocytes produced CD41+ EVs, including miRs, that were received by endothelial cells to induce the expression of angiogenesis-related genes. These results indicated that the CD41+ EVs produced from thrombocytes act as signaling molecules to repair damaged blood vessels.
放射線照射後の骨髄細胞が産生する新規造血幹細胞増殖因子の探索
Grant number:13770577
2001 - 2002
System name:科学研究費助成事業
Research category:若手研究(B)
Awarding organization:日本学術振興会
杉本 健吉
Grant amount:\1200000 ( Direct Cost: \1200000 )
致死量X線マウスの骨髄・脾臓・肝臓におけるサイトカイン等の発現をRT-PCR法で確認するとともに、血清中におけるサイトカインの濃度をELISA法で測定した結果、有意に遺伝子の発現が上昇したのはSCFであったが、血清中のSCFの増加はflt3-ligandほど顕著ではなかった。ただし、いずれのサイトカインも生体外幹細胞増殖で用いられる濃度に比してはるかに低い濃度であった。SCFに関しては膜結合型のSCFの発現が誘導されたことが判明した。実際のSca-1/c-kit両陽性細胞(造血幹細胞)の動態をセルソーターを用いて解析し、いつ増幅が起きているのかを把握した。骨髄では骨髄移植後4日目から増加し始め9日目には正常時に比べ約13倍に増加し、増減を繰り返しながら30日目までにほぼ正常値に戻った。増殖期のSca-1/c-kit両陽性細胞の細胞周期はおよそ14時間であった。増殖期造血幹細胞におけるサイトカインレセプターの発現を磁気分離法によって選別したSca-1陽性細胞でPCRにより検討した結果、生体外幹細胞増殖に用いられるサイトカインレセプター(c-kit, IL-6R, flt-3等)およびtie-2の発現は認められるものの、発現が増強されているものは認められなかった。またCD34,GM-CSFRの発現は認められなかった。さらに造血幹細胞増殖阻害因子であるMIP-1αの骨髄細胞での発現を検討したが、発現は認められるものの顕著な変化は認められなかった。したがって放射線照射後に誘導される造血幹細胞の迅速な増殖は、微小環境中の造血幹細胞増殖抑制因子の産生量が低下するために引き起こされるのではなく、既存のサイトカイン以外に新たに誘導される支持細胞が産生する膜結合タイプのサイトカインが関与している可能性が強く示唆された(論文投稿中)。
造血因子依存性細胞に造血支持細胞との直接接触によって発現誘導される遺伝子の検索
Grant number:08740650
1996
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
杉本 健吉
Grant amount:\900000 ( Direct Cost: \900000 )
研究計画では造血因子依存性細胞に造血支持細胞との直接接触によって誘導される未知の遺伝子を見つけるまで遂行する予定であったが、平成9年3月現在の状況はサブトラクション後のクローンをスクリーニングしている段階で、未知の遺伝子の発見には至っていない。研究の遅延の原因には以下のようなものがあった。
1.サブトラクション後のcDNA増幅がうまくいかなかった。他社製品に比べて比較的安い耐熱性DNA合成酵素を汎用していたがうまく増幅できなかったため、Taq酵素への変更やその他のPCRコンディションを検討することにより、かなりの高分子のcDNAも増幅することが出来るようになった。
2.サブトラクテッドcDNAのライブラリー構築が困難であった。通常のラムダファージベクターの組み込みは、何回かチャレンジしたがうまくいかなかった。そこでTA cloningを試みたがインサート含むクローンが極端に少なかった。残念ながら理由の解明には至っていない。そこで方法を変え制限酵素処理/脱リン酸化したベクターにインサートを組み込む基本的な方法によってインサートを持つクローンを幾つか得ることができた。
3.実際の実験を遂行する上で幾つかの点の改良を試みた。
1)サブトラクション時に共培養後のDA-1由来のcDNAから更にMS-5由来のcDNAを引く操作をつけ加え、共培養後のDA-1に特異的なcDNAの割合を高めた。
2)サブトラクテッドクローンのノーザンハイブリダイゼーションによるスクリーニング操作を行い、大量クローンの処理に対応出来るようにした。
今後の計画
スクリーニング後ポジティブクローンを得たら、この遺伝子配列を決定し既知の遺伝子と同一であるかを検討すると共に、動物細胞発現ベクターにサブクローニングし、共培養前のDA-1細胞に組み込みその機能を調べる予定である。
Ageing mechanisms of hemopoietic system in senescence accelerated (SAM-P) mice.
Grant number:05834004
1993 - 1994
System name:Grants-in-Aid for Scientific Research
Research category:Grant-in-Aid for General Scientific Research (C)
Awarding organization:Japan Society for the Promotion of Science
MORI Kazuhiro, SUGIMOTO Kenkichi
Grant amount:\2200000 ( Direct Cost: \2200000 )
Senescence accelerated (SAM-p) mice show a significant decrease in the number of peripheral blood cells with age. Mutual bone marrow transplantation between young and old mice of this strain revealed that the differentiation-supporting function of the spleen of the old mice was less active than that in the young mice.
To elucidate the possible decrease in cytokine production by the hemopoietic microenvironmet of the old mice, we have assayd the production of granulocyte-macrophage colony stimulating factor (GM-CSF) in the young and old SAM-P mice.
Old mice produced much less GM-CSF compared to young mice. Degree of the response to IL-E stimulation, which is known to induce GM-CSF production by the murine bone marrow cells, was not different between young and old mice, although the total amount of GM-CSF was less in the old mice than that in the young mice in this case, as well.
There was no difference in the level of interleukin-6 production between young and old mice.
These results suggest that age-related decrease in cytokine production is responsible for the decreased production of peripheral blood cells in the old SAM-P mice.
インターロイキン3活性抑制因子の精製
Grant number:05780455
1993
System name:科学研究費助成事業
Research category:奨励研究(A)
Awarding organization:日本学術振興会
杉本 健吉
Grant amount:\900000 ( Direct Cost: \900000 )
今年度の実施結果
1.大量サンプルの精製に先だって少量のサンプルより精製ステップの検討を行い、硫安分画、疎水クロマトグラフ、陰イオンクロマトグラフ(1)、陽イオンクロマトグラフ、までの精製ステップを確立した。
2.次に陰イオンクロマトグラフ(2)で得られたいくつかの分画を更にゲル濾過クロマトグラフにかけてメジャーピークを単離したが、この段階でNIL-3活性は分散してしまった。この段階の幾つかのサンプルについてアミノ酸配列を決定したところ、プレアルブミンであることが判明した。
3.そこでプレアルブミンのNIL-3活性について検討したが、プレアルブミンにNIL-3活性は検出できなかった。但し、マウスのプレアルブミンは市販品が存在しない為、ヒトのプレアルブミンを使用した。
4.最終段階にまで精製を進めるため、NIL-3の精製源を約10リットル集めた。
今後の展開に関する計画
1.陰イオンクロマトグラフ(2)の段階で活性分画が分散してしまったので、メジャーピークとして活性分画を得るための条件を再検討する。
この条件検討の為に、ある程度のサンプル量を準備中である。
2.今回の予備精製で、最終段階近くまでプレアルブミンがメジャーピーク中に混ざってくることが判明したので、プレアルブミン除去の方法を検討する。
3.マウスのプレアルブミンについてもNIL-3活性を検討する必要がある。
4.最終段階までの精製めどがついたところで、サンプル取得時に得られた細胞よりライブラリーを構築する準備を進める。
冬眠制御
Grant type:Competitive
造血制御
Grant type:Competitive
Regulation of Hibernation
Grant type:Competitive
Regulation of Hematopoiesis
Grant type:Competitive
生物学実習IV
医学検査機器概論
生物学実習I
科学・技術と社会
日本事情自然系A
生物学実験
理学スタディ・スキルズ
生命科学への招待(生物学学習法)
基礎生物科学実習II
基礎生物科学実習I
生物学基礎演習
遺伝子工学概論
細胞分化制御学特論
医学検査機器概論
生物学特論 VI
理学スタディ・スキルズ
自然科学基礎実験
課題研究II(生物学)
生物学基礎実習a
動物分子生理学実習
生物学総合演習
課題研究I(生物学)
生物学基礎実習b
安全教育
生物学特論 III
基礎生命科学セミナー
外国語論文解説・討論Ⅰ
研究発表(博士)演習(学会発表含む)
生命・食料科学博士セミナーⅠ
生命・食料科学博士特定研究Ⅰ
生物学特論Ⅳ
動物生理学II
基礎生物科学実習I
課題研究I(生物学科)
研究発表演習(中間発表)
生命・食料科学セミナーBⅠ
文献詳読Ⅰ
生命・食料科学特定研究BⅡ
文献詳読Ⅱ
生命・食料科学特定研究BⅠ
生命・食料科学セミナーBⅡ
研究発表
生命科学への招待(生物学学習法)
生物学基礎演習
日本事情自然系A
進化生物学II
自然科学総論Ⅳ
分子生物学演習
生物学実験 I
生物学基礎B
分子生物学
基礎生物科学実習II
細胞分化制御学特論
遺伝子工学概論
分子生物学実習
課題研究II
動物機能制御学
課題研究I
細胞生物学演習