研究者詳細 - 竹中　彰治

論文 - 竹中　彰治

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Hyperglycemia in obese type 2 diabetes mellitus rats affects pulp wound healing

Rosa Baldeon-Gutierrez, Naoto Ohkura, Nagako Yoshiba, Shintaro Takahara, Susan Gomez-Kasimoto, Takako Ida, Naoki Edanami, Kunihiko Yoshiba, Shoji Takenaka, Yuichiro Noiri

JOURNAL OF ORAL BIOSCIENCES 67 ( 4 ) 2025年12月

異種歯乳頭由来幹細胞を用いた再生歯内療法における有用性の評価

高原信太郎, 大倉直人, Baldeon-Gutierrez Rosa, Gomez-Kasimoto Susan, Thongtade Pemika, 竹中彰治, 廣瀬陽菜, 吉羽永子, 松崎英津子, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 163回 79 - 79 2025年10月

Role of Dystrophic Calcification in Reparative Dentinogenesis After Rat Molar Pulpotomy. 国際誌

Naoki Edanami, Kunihiko Yoshiba, Razi Saifullah Ibn Belal, Nagako Yoshiba, Shoji Takenaka, Naoto Ohkura, Shintaro Takahara, Takako Ida, Rosa Baldeon, Susan Kasimoto, Pemika Thongtade, Yuichiro Noiri

International journal of molecular sciences 26 ( 15 ) 2025年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Vital pulp therapy with calcium hydroxide or mineral trioxide aggregate (MTA) rapidly induces dystrophic calcification and promotes the accumulation of two members of small integrin-binding ligand N-linked glycoproteins: osteopontin (OPN) and dentin matrix protein-1 (DMP1). However, the precise relationship between these initial events and their roles in reparative dentinogenesis remain unclear. This study aimed to clarify the relationship between dystrophic calcification, OPN and DMP1 accumulation, and reparative dentin formation. Pulpotomy was performed on rat molars using MTA or zirconium oxide (ZrO2). ZrO2 was used as a control to assess pulp healing in the absence of dystrophic calcification. Pulpal responses were evaluated from 3 h to 7 days postoperatively via elemental mapping, micro-Raman spectroscopy, and histological staining. In the MTA-treated group, a calcium-rich dystrophic calcification zone containing calcite and hydroxyapatite was observed at 3 h after treatment; OPN and DMP1 accumulated under the dystrophic calcification zone by day 3; reparative dentin formed below the region of OPN and DMP1 accumulation by day 7. In contrast, these reactions did not occur in the ZrO2-treated group. These results suggest that dystrophic calcification serves as a key trigger for OPN and DMP1 accumulation and plays a pivotal role in reparative dentinogenesis.

DOI： 10.3390/ijms26157130

歯根膜における5型コラーゲンの組織学的解析

井田貴子, 大倉直人, Gomez Kasimoto Susan Kiara, Baldeon-Gutierrez Rosa, 枝並直樹, 竹中彰治, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 162回 140 - 140 2025年6月

歯周病原細菌が産生する外膜小胞の血行性胎盤移行と胎盤栄養膜細胞への内在化

山本倫子, 大倉直人, 稲垣舞, 竹中彰治, 近藤もも香, 立川正憲

日本薬剤学会年会講演要旨集 40年会 133 - 133 2025年5月

Influence of Tooth Maturity on Healing Outcomes in Regenerative Endodontics

S. Takahara, N. Ohkura, N. Yoshiba, R. Baldeon-Gutierrez, S. Gomez-Kasimoto, N. Edanami, T. Ida, S. Takenaka, K. Yoshiba, Y. Noiri

Journal of Dental Research 2025年4月

掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE Publications

Regenerative endodontic procedures using blood clots (BC-REP) for immature teeth typically exhibit a periodontal ligament–like healing pattern. However, a pulp-like healing pattern is observed in the presence of residual pulp. This study aimed to clarify the healing phenotype according to tooth maturity when performing BC-REP in the presence of residual pulp, focusing on migrated mesenchymal stem/stromal cells (MSCs). BC-REP rat molar models were created in the presence of residual pulp at ages corresponding to tooth developmental stages, from immature to mature (5 wk: immature, root is still growing; 8 wk: near mature, root has finished growing in length but the apex is not formed; 11 wk: mature, the apex is formed). The healing pattern and histological MSC markers (α–smooth muscle actin [α-SMA], CD73, CD90, and CD146) were investigated. The frequency of periodontal ligament–like healing was higher in mature teeth than in immature teeth. In addition, more healing macrophages were observed at the apical site 28 d after BC-REP, which is the final stage of healing. In immature teeth, double-immunopositive cells for proliferation markers (ki67 and proliferating cell nuclear antigen [PCNA]) and α-SMA were frequently observed in the vicinity of the root canal orifice 7 d after treatment, which is the early stage of healing. By contrast, in mature teeth, the number of CD73-, CD90-, and CD146-immunopositive cells increased at the apical site after 7 and 28 d. CD90- and CD146-immunopositive cells expressed cell proliferation markers (ki67 or PCNA) after 7 d. MSC migration after BC-REP likely varies based on tooth maturity, resulting in different healing phenotypes.

DOI： 10.1177/00220345251325826

その他リンク： https://journals.sagepub.com/doi/full-xml/10.1177/00220345251325826

ヒト血液脳関門を模倣したマイクロ流体モデルにおける歯周病原性細菌由来ナノ粒子の取り込み動態

近藤もも香, 万々桜, 大倉直人, 竹中彰治, 梶弘和, 稲垣舞, 立川正憲

生体医歯工学共同研究拠点成果報告書 2024年度 61 - 61 2025年4月

An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars. 国際誌

Shintaro Takahara, Naoki Edanami, Razi Saifullah Ibn Belal, Kunihiko Yoshiba, Shoji Takenaka, Naoto Ohkura, Nagako Yoshiba, Susan Gomez-Kasimoto, Yuichiro Noiri

Journal of functional biomaterials 16 ( 1 ) 2025年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

This study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) and Nishika Canal Sealer BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial root canals of the upper first molars were pulpectomized and overfilled with EBC, NBG, or, as reference, epoxy resin-based AH Plus (AHP). After 28 days, periapical tissue reactions were assessed using microcomputed tomography and histological staining. The elemental composition and chemical composition of the extruded EBC and NBG were analyzed at Day 1 and 28 using an electron probe microanalyzer and micro-Raman spectroscopy. No periapical lesions were observed with the sealer extrusion. Additionally, inflammation around the extruded EBC and NBG was minimal to mild on Day 28, whereas moderate inflammation was found around the extruded AHP. Silicon concentration in the extruded EBC and NBG decreased significantly from Day 1 to 28, with almost no silicon present on Day 28. Furthermore, the extruded EBC and NBG became calcium- and phosphorus-rich, showing a Raman band for hydroxyapatite on Day 28. In conclusion, EBC and NBG demonstrated favorable biocompatibility and the ability to release silicon elements and produce hydroxyapatite when extruded into the periapical tissues. AHP showed moderate periapical tissue irritancy.

DOI： 10.3390/jfb16010014

Interaction between the human dental microbiome and host gingival model

Maki Sotozono, Ryouhei Takahashi, Takako Ida, Ryoko Nagata, Rui Saito, Niraya Kornsombut, Jutharat Manuschai, Shoji Takenaka, Yuichiro Noiri

Microbiota and Host 3 ( 1 ) 2024年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：Bioscientifica

Objective

Interactions between the dental biofilm and host are important to study infectious oral diseases such as dental caries and periodontitis. Previous studies have reported the reaction of a host gingival model cocultured with bacteria or biofilms. However, these studies only report results of a gingival model, and a biofilm cocultured with a gingival model has never been investigated, although the microbiome of dental biofilms is associated with the maintenance of health and the onset of infectious oral diseases. Therefore, we aimed to investigate the reaction of a gingival model and experimental biofilm. An in situ dental biofilm model, which enables biofilm formation in the oral cavity, was used to prepare experimental biofilms.

Methods

The in situ biofilm was cocultured with a reconstructed human gingival (RHG) model. We performed 16S rRNA sequence analysis, metagenomic function prediction analysis, total bacterial cell counts using quantitative polymerase chain reaction and confocal laser microscopy observation in addition to gene expression and histological investigation of RHG.

Results

We found a change in Veillonella abundance in the in situ biofilms cocultured with RHG. Metagenomic function prediction analysis revealed that the activity of certain pathways associated with amino acid production, lactic acid fermentation and the glycolysis system significantly increased in biofilms cocultured with RHG.

Conclusion

The microbiome and functions of dental biofilms can be affected by host gingival tissues. Our findings will contribute to the establishment of a host–biofilm interaction model to understand the pathophysiology of oral infectious diseases.

DOI： 10.1530/mah-24-0008

その他リンク： https://mah.bioscientifica.com/downloadpdf/journals/mah/3/1/MAH-24-0008.xml

各種受容細胞におけるP.gingivalis由来外膜小胞の取り込み動態解析

大倉直人, 吉羽永子, 立川正憲, 中馬吉郎, Baldeon Gutierrez Rosa Edith, 高原信太郎, Gomez Kasimoto Susan, 井田貴子, 枝並直樹, 竹中彰治, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 161回 150 - 150 2024年11月

Effects of Tooth Desensitizers on Streptococcus mutans Biofilm Formation Using a Modified Robbins Device Flow Cell System

Niraya Kornsombut, Shoji Takenaka, Jutharat Manuschai, Maki Sotozono, Ryoko Nagata, Takako Ida, Risako Sato, Rui Saito, Ryouhei Takahashi, Daichi Sato, Yuichiro Noiri

International Journal of Molecular Sciences 25 ( 19 ) 10703 - 10703 2024年10月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study aimed to assess the antibiofilm effects of dentin desensitizers using a modified Robbins device flow cell system. The test desensitizers were Saforide, Caredyne Shield, and Clinpro White Varnish. Standardized dentin specimens were prepared from human single-rooted premolars, treated with one of the materials, and mounted on the modified Robbins device flow cell system. Streptococcus mutans biofilms were developed for 24 h at 37 °C under anaerobic conditions. Scanning electron microscopy, fluorescence confocal laser scanning microscopy, viable and total cell counts, acid production, and gene expression analyses were performed. A wavelength-dispersive X-ray spectroscopy electron probe microanalyzer was used to analyze the ion incorporations. Clinpro White Varnish showed the greatest inhibition, suggesting its suppression of bacterial adherence and transcription of genes related to biofilm formation. Saforide reduced only the number of viable bacteria, but other results showed no significant difference. The antibiofilm effects of Caredyne Shield were limited. The uptake of ions released from a material into dentin varies depending on the element. Clinpro White Varnish is effective for the short-term treatment of tooth sensitivity due to dentin demineralization. It prioritizes remineralization by supplying calcium and fluoride ions while resisting biofilm formation.

DOI： 10.3390/ijms251910703

In Vitro Inhibitory Effect of Silver Diamine Fluoride Combined with Potassium Iodide against Mixed-Species Biofilm Formation on Human Root Dentin 査読

Manuschai J., Sotozono M., Takenaka S., Kornsombut N., Takahashi R., Saito R., Nagata R., Ida T., Noiri Y.

Antibiotics 13 ( 8 ) 2024年8月

歯髄創傷治癒および歯髄再生過程におけるリン酸トランスポーター(Pit-1)の免疫組織学的解析

大倉直人, Baldeon Gutierrez Rosa Edith, 高原信太郎, Gomez Kasimoto Susan Kiara, 枝並直樹, 井田貴子, 外園真規, 永田量子, 竹中彰治, 吉羽邦彦, 吉羽永子, 野杁由一郎

日本歯科保存学雑誌 67 ( 3 ) 165 - 173 2024年6月

A novel 12-membered ring non-antibiotic macrolide EM982 attenuates cytokine production by inhibiting IKKβ and IκBα phosphorylation

Rui Saito, Hisanori Domon, Takumi Hiyoshi, Satoru Hirayama, Tomoki Maekawa, Shoji Takenaka, Yuichiro Noiri, Akari Ikeda, Tomoyasu Hirose, Toshiaki Sunazuka, Yutaka Terao

Journal of Biological Chemistry 107384 - 107384 2024年5月

In situデンタルバイオフィルムモデルを用いた宿主バイオフィルム間相互作用の解明

高橋竜平, 外園真規, 井田貴子, 永田量子, Kornsombut Niraya, 齋藤瑠郁, 竹中彰治, 野杁由一郎

BACTERIAL ADHERENCE & BIOFILM 37 35 - 41 2024年5月

Antibiofilm Properties and Demineralization Suppression in Early Enamel Lesions Using Dental Coating Materials 査読

Niraya Kornsombut, Shoji Takenaka, Maki Sotozono, Ryoko Nagata, Takako Ida, Jutharat Manuschai, Rui Saito, Ryouhei Takahashi, Yuichiro Noiri

Antibiotics 13 106 2024年1月

担当区分：責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study aimed to investigate the effects of dental coating materials on Streptococcus mutans biofilm formation. The test materials were PRG Barrier Coat (PRG), BioCoat Ca (BioC), and FluorDental Jelly (FluorJ). Bovine enamel specimens were demineralized to mimic early enamel lesions. The biofilm was developed on a specimen treated with one of the materials by using a modified Robbins device flow-cell system. Scanning electron and fluorescence confocal laser scanning microscopy, viable and total cell counts, and gene expression assessments of the antibiofilm were performed. Ion incorporation was analyzed using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer. All materials allowed biofilm formation but reduced its volume. FluorJ was the only material that inhibited biofilm accumulation and had a bactericidal effect, revealing 0.66 log CFU in viable cells and 1.23 log copy reduction in total cells compared with the untreated group after 24 h of incubation. The ions released from PRG varied depending on the element. BioC contributed to enamel remineralization by supplying calcium ions while blocking the acid produced from the biofilm. In summary, the dental coating materials physically prevented acid attacks from the biofilm while providing ions to the enamel to improve its mechanical properties.

DOI： 10.3390/antibiotics13010106

再生歯内療法後の治癒過程初期における各種間葉系幹細胞マーカー陽性細胞の局在性

高原信太郎, 大倉直人, 吉羽永子, 竹中彰治, 枝並直樹, 吉羽邦彦, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 159回 47 - 47 2023年10月

歯根形成時におけるTissue nonspecific alkaline phosphataseの機能解析

大倉直人, 吉羽永子, 高原信太郎, Baldeon Gutierrez Rosa Edith, Gomez-Kasimoto Susan, 井田貴子, 枝並直樹, 竹中彰治, 吉羽邦彦, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 159回 92 - 92 2023年10月

Preparing of Point-of-Care Reagents for Risk Assessment in the Elderly at Home by a Home-Visit Nurse and Verification of Their Analytical Accuracy 査読

Shoji Takenaka, Hiroshi Moro, Utako Shimizu, Takeshi Koizumi, Kei Nagano, Naoki Edanami, Naoto Ohkura, Hisanori Domon, Yutaka Terao, Yuichiro Noiri

Diagnistics ahead of online ( 14 ) 2407 - 2407 2023年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

With the rising number of older adults residing at home, there is a growing need for risk assessment and patient management in home nursing. This study aims to develop point-of-care test (POCT) reagents that can aid in risk assessment and home care, especially in settings with limited resources. Our focus was on creating a C-reactive protein (CRP) POCT, which can accurately diagnose clinically significant judgment values in home nursing. Additionally, we assessed the utility of the HemoCue WBC DIFF system in providing differential counts of white blood cells (WBC). These performances were compared with a laboratory test using blood samples from patients with pneumonia. The CRP POCT showed a comparable result to that of a laboratory method, with an average kappa index of 0.883. The leukocyte count showed good agreement with the reference method. While the correlation coefficients for both neutrophil and lymphocyte counts were deemed acceptable, it was observed that the measured values tended to be smaller in cases where the cell count was higher. This proportional error indicates a weak correlation with the neutrophil-to-lymphocyte ratio. CRP POCT and WBC counts provided reliable and accurate judgments. These tools may benefit risk management for older adults at home, patients with dementia who cannot communicate, and those living in depopulated areas.

DOI： 10.3390/diagnostics13142407

歯髄再生療法モデルラットを用いた歯根成長段階による治癒形態の比較解析

高原信太郎, 大倉直人, 吉羽邦彦, 吉羽永子, 竹中彰治, 枝並直樹, 庭野和明, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 158回 31 - 31 2023年5月

在宅高齢者のリスクアセスメントを容易にする感染症迅速診断キットの開発(第一報) C-reactive protein(CRP)イムノクロマト試薬の開発と性能評価

竹中彰治, 枝並直樹, 齋藤瑠郁, 大倉直人, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 158回 148 - 148 2023年5月

In Vivo Assessment of the Apatite-Forming Ability of New-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model. 国際誌

Naoki Edanami, Shoji Takenaka, Razi Saifullah Ibn Belal, Kunihiko Yoshiba, Shintaro Takahara, Nagako Yoshiba, Naoto Ohkura, Yuichiro Noiri

Journal of functional biomaterials 14 ( 4 ) 2023年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating the hard-tissue inductivity of the materials. This study evaluated the in vivo apatite-forming ability of 13 new-generation HCSCs using an original HCSC (white ProRoot MTA: PR) as a positive control. The HCSCs were loaded into polytetrafluoroethylene tubes and implanted in the subcutaneous tissue of 4-week-old male Wistar rats. At 28 days after implantation, hydroxyapatite formation on the HCSC implants was assessed with micro-Raman spectroscopy, surface ultrastructural and elemental characterization, and elemental mapping of the material-tissue interface. Seven new-generation HCSCs and PR had a Raman band for hydroxyapatite (v1 PO43- band at 960 cm-1) and hydroxyapatite-like calcium-phosphorus-rich spherical precipitates on the surfaces. The other six HCSCs with neither the hydroxyapatite Raman band nor hydroxyapatite-like spherical precipitates did not show calcium-phosphorus-rich hydroxyapatite-layer-like regions in the elemental mapping. These results indicated that 6 of the 13 new-generation HCSCs possessed little or no ability to produce hydroxyapatite in vivo, unlike PR. The weak in vivo apatite-forming ability of the six HCSCs may have a negative impact on their clinical performance.

DOI： 10.3390/jfb14040213

Degradation of EGFR on lung epithelial cells by neutrophil elastase contributes to the aggravation of pneumococcal pneumonia

Toshihito Isono, Satoru Hirayama, Hisanori Domon, Tomoki Maekawa, Hikaru Tamura, Takumi Hiyoshi, Kridtapat Sirisereephap, Shoji Takenaka, Yuichiro Noiri, Yutaka Terao

Journal of Biological Chemistry 104760 - 104760 2023年4月

In Vivo Assessment of the Calcium Salt-Forming Ability of a New Calcium Silicate-Based Intracanal Medicament: Bio-C Temp. 国際誌

Naoki Edanami, Razi Saifullah Ibn Belal, Shoji Takenaka, Kunihiko Yoshiba, Rosa Edith Baldeon Gutierrez, Shintaro Takahara, Nagako Yoshiba, Naoto Ohkura, Yuichiro Noiri

Dentistry journal 11 ( 4 ) 2023年3月

記述言語：英語掲載種別：研究論文（学術雑誌）

Calcium salt precipitation induced by intracanal medicaments contributes to the formation of apical hard tissue during apexification. This study compared the calcium salt-forming ability of a new calcium silicate-based intracanal medicament (Bio-C Temp) with that of two commercial calcium hydroxide pastes (Calcipex Plane II and Vitapex) in a rat subcutaneous implantation model. Polytetrafluoroethylene tubes containing each of the three materials were subcutaneously implanted in 4-week-old male Wistar rats. After 28 days, the composition and amount of calcium salts formed at the material-tissue interface were assessed using micro-Raman spectroscopy, X-ray diffraction, and elemental mapping. The tested materials produced white precipitates that had Raman spectra with peaks corresponding to hydroxyapatite and calcite. X-ray diffraction detected hydroxyapatite formation on Calcipex Plane II and Vitapex implants, as well as calcite formation on all three materials. Elemental mapping revealed that Bio-C Temp generated significantly smaller calcium- and phosphorus-rich calcified regions within the subcutaneous connective tissue than Vitapex. These results indicate that Bio-C Temp produced less calcium salt in rat subcutaneous tissue than Vitapex, although all materials formed hydroxyapatite and calcite in rat subcutaneous tissue. Bio-C Temp could be less effective than Vitapex in promoting apical hard tissue formation during apexification.

DOI： 10.3390/dj11040091

Prostaglandin E2-Transporting Pathway and Its Roles via EP2/EP4 in Cultured Human Dental Pulp. 国際誌

Naoto Ohkura, Kunihiko Yoshiba, Nagako Yoshiba, Yohei Oda, Naoki Edanami, Hayato Ohshima, Shoji Takenaka, Takashi Okiji, Yuichiro Noiri

Journal of endodontics 49 ( 4 ) 410 - 418 2023年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

INTRODUCTION: Prostaglandin E2 (PGE2) exerts biological actions through its transport pathway involving intracellular synthesis, extracellular transport, and receptor binding. This study aimed to determine the localization of the components of the PGE2-transporting pathway in human dental pulp and explore the relevance of PGE2 receptors (EP2/EP4) to angiogenesis and dentinogenesis. METHODS: Protein localization of microsomal PGE2 synthase (mPGES), PGE2 transporters [(multidrug resistance-associated protein-4 (MRP4) and prostaglandin transporter (PGT)], and EP2/EP4 was analyzed using double immunofluorescence staining. Tooth slices from human third molars were cultured with or without butaprost (EP2 agonist) or rivenprost (EP4 agonist) for 1 week. Morphometric analysis of endothelial cell filopodia was performed to evaluate angiogenesis, and real-time polymerase chain reaction was performed to evaluate angiogenesis and odontoblast differentiation markers. RESULTS: MRP4 and PGT were colocalized with mPGES and EP2/EP4 in odontoblasts and endothelial cells. Furthermore, MRP4 was colocalized with mPGES and EP4 in human leukocyte antigen-DR-expressing dendritic cells. In the tooth slice culture, EP2/EP4 agonists induced significant increases in the number and length of filopodia and mRNA expression of angiogenesis markers (vascular endothelial growth factor and fibroblast growth factor-2) and odontoblast differentiation markers (dentin sialophosphoprotein and collagen type 1). CONCLUSIONS: PGE2-producing enzyme (mPGES), transporters (MRP4 and PGT), and PGE2-specific receptors (EP2/EP4) were immunolocalized in various cellular components of the human dental pulp. EP2/EP4 agonists promoted endothelial cell filopodia generation and upregulated angiogenesis- and odontoblast differentiation-related genes, suggesting that PGE2 binding to EP2/EP4 is associated with angiogenic and dentinogenic responses.

DOI： 10.1016/j.joen.2023.01.009

Analysis of Genetic Relatedness between Gastric and Oral Helicobacter pylori in Patients with Early Gastric Cancer Using Multilocus Sequence Typing

Ryoko Nagata, Hiroki Sato, Shoji Takenaka, Junji Yokoyama, Shuji Terai, Hitomi Mimuro, Yuichiro Noiri

International Journal of Molecular Sciences 24 ( 3 ) 2211 - 2211 2023年1月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

The oral cavity is the second most colonized site of Helicobacter pylori after the stomach. This study aimed to compare the genetic relatedness between gastric and oral H. pylori in Japanese patients with early gastric cancer through multilocus sequence typing (MLST) analysis using eight housekeeping genes. Gastric biopsy specimens and oral samples were collected from 21 patients with a fecal antigen test positive for H. pylori. The number of H. pylori allelic profiles ranged from zero to eight since the yield of DNA was small even when the nested PCR was performed. MLST analysis revealed that only one patient had a matching oral and gastric H. pylori genotype, suggesting that different genotypes of H. pylori inhabit the oral cavity and gastric mucosa. The phylogenetic analysis showed that oral H. pylori in six patients was similar to gastric H. pylori, implying that the two strains are related but not of the same origin, and those strains may be infected on separate occasions. It is necessary to establish a culture method for oral H. pylori to elucidate whether the oral cavity acts as the source of gastric infection, as our analysis was based on a limited number of allele sequences.

DOI： 10.3390/ijms24032211

SVCT2-GLUT1-mediated ascorbic acid transport pathway in rat dental pulp and its effects during wound healing 査読国際誌

Naoto Okura, Kunihiko Yoshiba, Nagako Yoshiba, Shoji Takenaka, Naoki Edanami, Hayato Oshima, Yuichiro Noiri

Scientific Reports ahead of online ( 1 ) 1251 - 1251 2023年1月

Efficacy of Combining an Extraoral High-Volume Evacuator with Preprocedural Mouth Rinsing in Reducing Aerosol Contamination Produced by Ultrasonic Scaling. 査読国際誌

Shoji Takenaka, Maki Sotozono, Asaka Yashiro, Rui Saito, Niraya Kornsombut, Traithawit Naksagoon, Ryoko Nagata, Takako Ida, Naoki Edanami, Yuichiro Noiri

International journal of environmental research and public health 19 ( 10 ) 2022年5月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

The coronavirus disease pandemic has afforded dental professionals an opportunity to reconsider infection control during treatment. We investigated the efficacy of combining extraoral high-volume evacuators (eHVEs) with preprocedural mouth rinsing in reducing aerosol contamination by ultrasonic scalers. A double-masked, two-group, crossover randomized clinical trial was conducted over eight weeks. A total of 10 healthy subjects were divided into two groups; they received 0.5% povidone-iodine (PI), essential oil (EO), or water as preprocedural rinse. Aerosols produced during ultrasonic scaling were collected from the chest area (PC), dentist's mask, dentist's chest area (DC), bracket table, and assistant's area. Bacterial contamination was assessed using colony counting and adenosine triphosphate assays. With the eHVE 10 cm away from the mouth, bacterial contamination by aerosols was negligible. With the eHVE 20 cm away, more dental aerosols containing bacteria were detected at the DC and PC. Mouth rinsing decreased viable bacterial count by 31-38% (PI) and 22-33% (EO), compared with no rinsing. The eHVE prevents bacterial contamination when close to the patient's mouth. Preprocedural mouth rinsing can reduce bacterial contamination where the eHVE is positioned away from the mouth, depending on the procedure. Combining an eHVE with preprocedural mouth rinsing can reduce bacterial contamination in dental offices.

DOI： 10.3390/ijerph19106048

Comparison of calcium and hydroxyl ion release ability and in vivo apatite-forming ability of three bioceramic-containing root canal sealers. 査読国際誌

Razi Saifullah Ibn Belal, Naoki Edanami, Kunihiko Yoshiba, Nagako Yoshiba, Naoto Ohkura, Shoji Takenaka, Yuichiro Noiri

Clinical oral investigations 26 ( 2 ) 1443 - 1451 2022年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Science and Business Media LLC

OBJECTIVE: Bioceramic-containing root canal sealers promote periapical healing via Ca2+ and OH- release and apatite formation on the surface. This study aimed to compare Ca2+ and OH- release and in vivo apatite formation of three bioceramic-containing root canal sealers: EndoSequence BC sealer (Endo-BC), MTA Fillapex (MTA-F), and Nishika Canal Sealer BG (N-BG). MATERIALS AND METHODS: Polytetrafluoroethylene tubes filled with sealers were immersed in distilled water for 6 and 12 h and for 1, 7, 14, and 28 days to measure Ca2+ and OH- release. Additionally, tubes filled with sealers were implanted in the backs of rats for 28 days, and in vivo apatite formation was analyzed using an electron probe microanalyzer. RESULTS: Endo-BC released significantly more Ca2+ than the other sealers at 6 and 12 h and 1 day. Ca2+ release was significantly lower from N-BG than from Endo-BC and MTA-F at 14 and 28 days. OH- release was significantly higher from Endo-BC than from the other sealers throughout the experiment, except at 1 day. OH- release was lower from N-BG than from MTA-F at 6 h and 7 days. Only Endo-BC implants exhibited apatite-like calcium-, phosphorus-, oxygen-, and carbon-rich spherulites and apatite layer-like calcium- and phosphorus-rich, but radiopaque element-free, surface regions. CONCLUSIONS: Ca2+ and OH- release is ranked as follows: Endo-BC > MTA-F > N-BG. Only Endo-BC demonstrated in vivo apatite formation. CLINICAL RELEVANCE: Endo-BC could promote faster periapical healing than MTA-F and N-BG.

DOI： 10.1007/s00784-021-04118-w

その他リンク： https://link.springer.com/article/10.1007/s00784-021-04118-w/fulltext.html

Evidence on the use of mouthwash for the control of supragingival biofilm and its potential adverse effects 査読国際誌

Takenaka S, Sotozono S, Ohkura N, Noiri Y

Antibiotics 11 ( Ahead of online ) 2022年

Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis. 査読国際誌

Shoji Takenaka, Naoki Edanami, Yasutaka Komatsu, Ryoko Nagata, Traithawit Naksagoon, Maki Sotozono, Takako Ida, Yuichiro Noiri

Microorganisms 9 ( 11 ) 2021年11月

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

We performed a comprehensive microbiome analysis of root caries lesions using 22 teeth extracted from patients with severe periodontitis. The carious lesions were mechanically collected and cryo-pulverized following tooth extraction. Differences in the microbiome were compared between independent lesions at the supragingival site (SG) and lesions extending beyond the gingival margin (GCB). DNA was extracted and the microbiome was characterized on the basis of the V3-V4 hypervariable region of the 16S rRNA gene using paired-end sequencing on an Illumina MiSeq device. The microbiota in root caries lesions showed compositionally distinct microbiota depending on the location. The most abundant OTUs in the SG group were Streptococcus (26.0%), Actinomyces (10.6%), and Prevotella (7.6%). GCB presented Prevotella (11.1%) as the most abundant genus, followed by Fusobacterium (9.6%) and Actinomyces (8.7%). The SG group showed a lack of uniformity in microbiota compared with the GCB group. The bacterial profiles of GCB varied considerably among patients, including periodontal pathogens such as Porphyromonas, Selenomonas, Filifactor, Peptococcus, and Tannerella. Periodontal pathogens inhabit root caries lesions that extend beyond the gingival margin. This study provides a new perspective for elucidating the microbial etiology of root caries.

DOI： 10.3390/microorganisms9112349

Effect of a resin-modified calcium silicate cement on inflammatory cell infiltration and reparative dentin formation after pulpotomy in rat molars. 査読国際誌

Naoki Edanami, Razi Saifullah Ibn Belal, Kunihiko Yoshiba, Nagako Yoshiba, Naoto Ohkura, Shoji Takenaka, Yuichiro Noiri

Australian endodontic journal : the journal of the Australian Society of Endodontology Inc 48 ( 2 ) 297 - 304 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Resin monomers and polymerisation initiators have been shown to be cytotoxic for pulp cells and to disturb odontoblast differentiation. This study aimed to compare the effect of a resin-modified calcium silicate cement (TheraCal LC; TC) and a resin-free calcium silicate cement (ProRoot MTA; PR) on pulpal healing after pulpotomy. Pulpotomy was performed on the maxillary first molars of 8-week-old rats using either PR or TC. After 1, 3, 7, 14 and 28 days, pulpal responses were assessed by micro-computed tomography, haematoxylin-eosin staining and immunostaining against CD68, which is a pan-macrophage marker. The results showed that pulpotomy with TC induced persistent infiltration of inflammatory cells, including CD68-positive macrophages, and delayed the formation of reparative dentin as compared with that with PR, although both materials allowed pulpal healing over the long term. Therefore, resin-modified TC was not as biocompatible nor bioinductive as resin-free PR when applied on the healthy pulp of rat molars.

DOI： 10.1111/aej.12568

Apatite-Forming Ability of Flowable vs. Putty Formulations of Newly Developed Bioactive Glass-Containing Endodontic Cement 査読

Naoki Edanami, Razi Saifullah Ibn Belal, Shoji Takenaka, Kunihiko Yoshiba, Nagako Yoshiba, Naoto Ohkura, Shintaro Takahara, Yuichiro Noiri

Applied Sciences 11 ( 19 ) 8969 - 8969 2021年9月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study compared the apatite-forming ability (AFA) levels of flowable and putty formulations of Nishika Canal Sealer BG Multi (F-NBG and P-NBG, respectively) and attempted to clarify the cause of differences in the AFA levels of F-NBG and P-NBG. NBG samples were aged in simulated body fluid (SBF) or 1-, 5-, or 10-g/L bovine serum albumin-containing SBF (BSA-SBF) and analyzed in terms of their ultrastructures, elemental compositions, and Raman spectra to identify apatite formation. The phosphate ion consumption rates of NBG samples in the media were evaluated as an indicator of apatite growth. The original elemental composition, calcium ion release, and alkalizing ability levels of F-NBG and P-NBG were also evaluated. Apparent apatite formation was detected on all NBG samples except F-NBG aged in 10-g/L BSA-SBF. P-NBG consumed phosphate ions faster than F-NBG. As-prepared P-NBG showed more silicon elements on its surface than as-prepared F-NBG. P-NBG released more calcium ions than F-NBG, although their alkalizing ability levels did not differ statistically. In conclusion, the AFA of P-NBG was greater than that of F-NBG, probably because of the greater ability of P-NBG to expose silanol groups on the surface and release calcium ions.

DOI： 10.3390/app11198969

A Repeated State of Acidification Enhances the Anticariogenic Biofilm Activity of Glass Ionomer Cement Containing Fluoro-Zinc-Silicate Fillers. 査読国際誌

Traithawit Naksagoon, Shoji Takenaka, Ryoko Nagata, Maki Sotozono, Tatsuya Ohsumi, Takako Ida, Naoki Edanami, Takeyasu Maeda, Yuichiro Noiri

Antibiotics (Basel, Switzerland) 10 ( 8 ) 2021年8月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

This study aimed to evaluate the anticariogenic biofilm activity of a novel zinc-containing glass ionomer cement, Caredyne Restore (CR), using a flow-cell system that reproduces Stephan responses. Streptococcus mutans biofilms were cultured on either CR or hydroxyapatite (HA) discs mounted on a modified Robbins device. The media were allowed to flow at a speed of 2 mL/min for 24 h while exposed to an acidic buffer twice for 30 min to mimic dietary uptake. Acid exposure enhanced biofilm inhibition in the CR group, which showed 2.6 log CFU/mm2 in viable cells and a 2 log copies/mL reduction in total cells compared to the untreated group after 24 h of incubation, suggesting enhanced anticariogenic activity due to the release of fluoride and zinc ions. However, there was no difference in the number of viable and total cells between the two experimental groups after 24 h of incubation in the absence of an acidic environment. The anticariogenic biofilm activity of CR occurs in acidic oral environments, for example in the transient pH drop following dietary uptake. CR restorations are recommended in patients at high risk of caries due to hyposalivation, difficulty brushing, and frequent sugar intake.

DOI： 10.3390/antibiotics10080977

Current Prevalence of Oral Helicobacter pylori among Japanese Adults Determined Using a Nested Polymerase Chain Reaction Assay. 査読国際誌

Ryoko Nagata, Tatsuya Ohsumi, Shoji Takenaka, Yuichiro Noiri

Pathogens (Basel, Switzerland) 10 ( 1 ) 10 2020年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

In Japan, gastric Helicobacter pylori infection prevalence has markedly decreased with socioeconomic development. We aimed to investigate the prevalence of oral H. pylori in Japanese adults in 2020 by sex, age, sampling site, and medical history. Unstimulated saliva, supragingival biofilm, and tongue coating were obtained from 88 subjects-with no complaints of upper digestive symptoms-attending a dentist's office for dental check-up or disorders. Supragingival biofilm was collected from the upper incisors, lower incisors, upper right molars and lower left molars to analyze the characteristic distribution. Oral H. pylori was detected using nested polymerase chain reaction. Oral H. pylori prevalence did not statistically differ by sex or age. Supragingival biofilm (30.7%) was the most common oral H. pylori niche; it was also detected in 4.5% of saliva and 2.3% of tongue samples. The lower incisor was the most common site among the supragingival biofilm samples, followed by the upper incisors, lower left molars, and upper right molars. Oral H. pylori DNA was frequently detected in patients with a history of gastric H. pylori infection. Oral H. pylori has a characteristic distribution independent of sex and age, suggesting that it is part of the normal microflora in the adult oral cavity.

DOI： 10.3390/pathogens10010010

Sulfated vizantin causes detachment of biofilms composed mainly of the genus Streptococcus without affecting bacterial growth and viability. 査読国際誌

Taisuke Hasegawa, Shoji Takenaka, Masataka Oda, Hisanori Domon, Takumi Hiyoshi, Karin Sasagawa, Tatsuya Ohsumi, Naoki Hayashi, Yasuko Okamoto, Hirofumi Yamamoto, Hayato Ohshima, Yutaka Terao, Yuichiro Noiri

BMC microbiology 20 ( 1 ) 361 - 361 2020年11月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Sulfated vizantin, a recently developed immunostimulant, has also been found to exert antibiofilm properties. It acts not as a bactericide, but as a detachment-promoting agent by reducing the biofilm structural stability. This study aimed to investigate the mechanism underlying this activity and its species specificity using two distinct ex vivo oral biofilm models derived from human saliva. RESULTS: The biofilm, composed mainly of the genus Streptococcus and containing 50 μM of sulfated vizantin, detached significantly from its basal surface with rotation at 500 rpm for only 15 s, even when 0.2% sucrose was supplied. Expression analyses for genes associated with biofilm formation and bacterial adhesion following identification of the Streptococcus species, revealed that a variety of Streptococcus species in a cariogenic biofilm showed downregulation of genes encoding glucosyltransferases involved in the biosynthesis of water-soluble glucan. The expression of some genes encoding surface proteins was also downregulated. Of the two quorum sensing systems involved in the genus Streptococcus, the expression of luxS in three species, Streptococcus oralis, Streptococcus gordonii, and Streptococcus mutans, was significantly downregulated in the presence of 50 μM sulfated vizantin. Biofilm detachment may be facilitated by the reduced structural stability due to these modulations. As a non-specific reaction, 50 μM sulfated vizantin decreased cell surface hydrophobicity by binding to the cell surface, resulting in reduced bacterial adherence. CONCLUSION: Sulfated vizantin may be a candidate for a new antibiofilm strategy targeting the biofilm matrix while preserving the resident microflora.

DOI： 10.1186/s12866-020-02033-w

Effect of water aging on the anti-biofilm properties of glass ionomer cement containing fluoro-zinc-silicate fillers. 査読国際誌

Traithawit Naksagoon, Tatsuya Ohsumi, Shoji Takenaka, Ryoko Nagata, Taisuke Hasegawa, Takeyasu Maeda, Yuichiro Noiri

Biofouling 36 ( 9 ) 1090 - 1099 2020年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

A previous study has reported that a novel fluoro-zinc-silicate glass ionomer cement (Caredyne Restore) showed superior anti-biofilm effects by interfering with bacterial adhesion. However, the active ions may degrade with time. This study aimed to assess the valid anti-biofilm effects of Caredyne Restore after being aged by water immersion for 3 weeks. Streptococcus mutans biofilm was allowed to grow on the surface before and after water aging for 24 h using a modified Robbins device flow-cell system. The results showed water aging promoted biofilm formation. Insufficient amount of fluoride and zinc ions were released from Caredyne Restore after water aging under neutral pH condition. An acidic pH is needed to exert effective anti-biofilm properties. As the release of active ions from Caredyne Restore will gradually decrease after the restoration, the restoration may not prevent biofilm formation after 3 weeks while neutral pH is maintained by the buffering capacity of saliva.

DOI： 10.1080/08927014.2020.1856371

Sulfated vizantin inhibits biofilm maturation by Streptococcus mutans. 査読国際誌

Masataka Oda, Mie Kurosawa, Hirofumi Yamamoto, Hisanori Domon, Shoji Takenaka, Tatsuya Ohsumi, Tomoki Maekawa, Naoto Yamasaki, Yui Furue, Yutaka Terao

Microbiology and immunology 64 ( 7 ) 493 - 501 2020年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Streptococcus mutans is the main pathogen of dental caries and adheres to the tooth surface via soluble and insoluble glucans produced by the bacterial glucosyltransferase enzyme. Thus, the S. mutans glucosyltransferase is an important virulence factor for this cariogenic bacterium. Sulfated vizantin effectively inhibits biofilm formation by S. mutans without affecting its growth. In this study, less S. mutans biofilm formation occurred on hydroxyapatite discs coated with sulfated vizantin than on noncoated discs. Sulfated vizantin showed no cytotoxicity against the human gingival cell line Ca9-22. Sulfated vizantin dose-dependently inhibited the extracellular release of cell-free glucosyltransferase from S. mutans and enhanced the accumulation of cell-associated glucosyltransferase, compared with that observed with untreated bacteria. Sulfated vizantin disrupted the localization balance between cell-associated glucosyltransferase and cell-free glucosyltransferase, resulting in inhibited biofilm maturation. These results indicate that sulfated vizantin can potentially serve as a novel agent for preventing dental caries.

DOI： 10.1111/1348-0421.12797

Adjunct use of mouth rinses with a sonic toothbrush accelerates the detachment of a Streptococcus mutans biofilm: an in vitro study. 査読国際誌

Tatsuya Ohsumi, Shoji Takenaka, Yuuki Sakaue, Yuki Suzuki, Ryoko Nagata, Taisuke Hasegawa, Hayato Ohshima, Yutaka Terao, Yuichiro Noiri

BMC oral health 20 ( 1 ) 161 - 161 2020年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: The aim of this in vitro study was to examine the possible enhancement of the biofilm peeling effect of a sonic toothbrush following the use of an antimicrobial mouth rinse. METHODS: The biofilm at a noncontact site in the interdental area was treated by sound wave convection with the test solution or by immersion in the solution. The biofilm peeling effect was evaluated by determining the bacterial counts and performing morphological observations. A Streptococcus mutans biofilm was allowed to develop on composite resin discs by cultivation with stirring at 50 rpm for 72 h. The specimens were then placed in recesses located between plastic teeth and divided into an immersion group and a combination group. The immersion group was treated with phosphate buffer, chlorhexidine digluconate Peridex™ (CHX) mouth rinse or Listerine® Fresh Mint (EO) mouth rinse. The combination group was treated with CHX or EO and a sonic toothbrush. RESULTS: The biofilm thickness was reduced by approximately one-half compared with the control group. The combination treatment produced a 1 log reduction in the number of bacteria compared to the EO immersion treatment. No significant difference was observed in the biofilm peeling effect of the immersion group compared to the control group. CONCLUSIONS: The combined use of a sonic toothbrush and a mouth rinse enhanced the peeling of the biofilm that proliferates in places that are difficult to reach using mechanical stress.

DOI： 10.1186/s12903-020-01144-0

Effects of a sub-minimum inhibitory concentration of chlorhexidine gluconate on the development of in vitro multi-species biofilms. 査読国際誌

Yuki Suzuki, Tatsuya Ohsumi, Toshihito Isono, Ryoko Nagata, Taisuke Hasegawa, Shoji Takenaka, Yutaka Terao, Yuichiro Noiri

Biofouling 36 ( 2 ) 146 - 158 2020年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Following antimicrobial administrations in oral environments, bacteria become exposed to a sub-minimum inhibitory concentration (sub-MIC), which can induce in vitro single-species biofilms. This study explored the effects of chlorhexidine gluconate (CHG) at a sub-MIC on in vitro multi-species biofilms comprising Streptococcus mutans, Streptococcus oralis and Actinomyces naeslundii. CHG at a sub-MIC was found to induce in vitro biofilm growth, although the bacterial growth was not significantly different from that in the control. The gene transcription related to S. mutans multi-species biofilm formation with CHG at a sub-MIC was significantly higher than that of the control, but this was not found in S. mutans single-species biofilms. The bio-volume of extracellular polysaccharides with CHG at a sub-MIC was significantly higher than that of the control. This suggests that CHG at a sub-MIC may promote the development of multi-species biofilms by affecting the gene transcription related to S. mutans biofilm formation.

DOI： 10.1080/08927014.2020.1739271

Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation 査読

Taisuke Hasegawa, Shoji Takenaka, Tatsuya Ohsumi, Takako Ida, Hayato Ohshima, Yutaka Terao, Traithawit Naksagoon, Takeyasu Maeda, Yuichiro Noiri

CLINICAL ORAL INVESTIGATIONS 24 ( 2 ) 963 - 970 2020年2月

Treatment of severe pneumonia by hinokitiol in a murine antimicrobial-resistant pneumococcal pneumonia model. 査読国際誌

Toshihito Isono, Hisanori Domon, Kosuke Nagai, Tomoki Maekawa, Hikaru Tamura, Takumi Hiyoshi, Katsunori Yanagihara, Eiji Kunitomo, Shoji Takenaka, Yuichiro Noiri, Yutaka Terao

PloS one 15 ( 10 ) e0240329 2020年

記述言語：英語掲載種別：研究論文（学術雑誌）

Streptococcus pneumoniae is often isolated from patients with community-acquired pneumonia. Antibiotics are the primary line of treatment for pneumococcal pneumonia; however, rising antimicrobial resistance is becoming more prevalent. Hinokitiol, which is isolated from trees in the cypress family, has been demonstrated to exert antibacterial activity against S. pneumoniae in vitro regardless of antimicrobial resistance. In this study, the efficacy of hinokitiol was investigated in a mouse pneumonia model. Male 8-week-old BALB/c mice were intratracheally infected with S. pneumoniae strains D39 (antimicrobial susceptible) and NU4471 (macrolide resistant). After 1 h, hinokitiol was injected via the tracheal route. Hinokitiol significantly decreased the number of S. pneumoniae in the bronchoalveolar lavage fluid (BALF) and the concentration of pneumococcal DNA in the serum, regardless of whether bacteria were resistant or susceptible to macrolides. In addition, hinokitiol decreased the infiltration of neutrophils in the lungs, as well as the concentration of inflammatory cytokines in the BALF and serum. Repeated hinokitiol injection at 18 h intervals showed downward trend in the number of S. pneumoniae in the BALF and the concentration of S. pneumoniae DNA in the serum with the number of hinokitiol administrations. These findings suggest that hinokitiol reduced bacterial load and suppressed excessive host immune response in the pneumonia mouse model. Accordingly, hinokitiol warrants further exploration as a potential candidate for the treatment of pneumococcal pneumonia.

DOI： 10.1371/journal.pone.0240329

A Horizontal Sequential Cutting Method to Estimate the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy. 査読国際誌

Taisuke Hasegawa, Shoji Takenaka, Rika Wakamatsu, Tatsuya Ohsumi, Hisanori Domon, Hayato Ohshima, Yutaka Terao, Yuichiro Noiri

Journal of endodontics 45 ( 9 ) 1142 - 1147 2019年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

INTRODUCTION: This study aimed to develop a technique to create sequential slices, allowing the fluorescent visualization of bacterial viability in all parts of an infected dentin. METHODS: Cylindrical dentin blocks were prepared from freshly extracted human teeth with a single-rooted canal. Each block was immersed in 5% sodium hypochlorite (NaOCl) and 17% EDTA for 5 minutes before being infected with Enterococcus faecalis. The bacteria were allowed to develop inside dentin specimens for 28 days under anaerobic conditions. The specimens were exposed in 2% NaOCl for either 2 minutes or 20 minutes at 20°C, 37°C, and 45°C, respectively. After staining with calcein AM (Thermo Fisher Scientific, Waltham, MA) and propidium iodide, the samples were cryoembedded, mounted on an adhesive film, and sectioned at a thickness of 10 μm along the running of the dentinal tubules. Stacks of fluorescent images were collected in the z dimension using confocal laser scanning microscopy, and the maximum affected distance from a root canal was measured from the 3-dimensional reconstructed image. The reliability of this technique was verified by comparison with a dye bleaching test. RESULTS: Horizontal sequential sections preserving 3-dimensional bacterial distribution and their viabilities could be made without decalcification. The treatment time contributed to the penetration of NaOCl into dentinal tubules, whereas temperature did not significantly affect the penetration. The judgment by confocal laser scanning microscopic analysis was consistent with that of a dye bleaching test. CONCLUSIONS: The horizontal sectioning method has the advantage of creating sequential sections, allowing information to be imaged at every portion.

DOI： 10.1016/j.joen.2019.06.004

Evidence-based strategy for dental biofilms: Current evidence of mouthwashes on dental biofilm and gingivitis 査読国際誌

Takenaka S, Ohsumi T, Noiri Y

Japanese Dental Science Review 55 ( 1 ) 33 - 40 2019年

The effect of chlorhexidine on dental calculus formation: An in vitro study 査読

Yuuki Sakaue, Shoji Takenaka, Tatsuya Ohsumi, Hisanori Domon, Yutaka Terao, Yuichiro Noiri

BMC Oral Health 18 ( 1 ) 52 2018年3月

Limitations and adverse influences of antimicrobial strategy for the control of oral biofilm. 査読

Takenaka S, Noiri Y

J Dent Oral Biol 2 ( 3 ) 1034 2017年

Vizantin inhibits bacterial adhesion without affecting bacterial growth and causes Streptococcus mutans biofilm to detach by altering its internal architecture 査読

Shoji Takenaka, Masataka Oda, Hisanori Domon, Tatsuya Ohsumi, Yuki Suzuki, Hayato Ohshima, Hirofumi Yamamoto, Yutaka Terao, Yuichiro Noiri

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 480 ( 2 ) 173 - 179 2016年11月

Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans 査読

Yuuki Sakaue, Hisanori Domon, Masataka Oda, Shoji Takenaka, Miwa Kubo, Yoshiyasu Fukuyama, Takashi Okiji, Yutaka Terao

MICROBIOLOGY AND IMMUNOLOGY 60 ( 1 ) 10 - 16 2016年1月

Residual Structure of Streptococcus mutans Biofilm following Complete Disinfection Favors Secondary Bacterial Adhesion and Biofilm Re-Development 査読

Tatsuya Ohsumi, Shoji Takenaka, Rika Wakamatsu, Yuuki Sakaue, Naoki Narisawa, Hidenobu Senpuku, Hayato Ohshima, Yutaka Terao, Takashi Okiji

PLOS ONE 10 ( 1 ) e0116647 2015年1月

Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization 査読

Rika Wakamatsu, Shoji Takenaka, Tatsuya Ohsumi, Yutaka Terao, Hayato Ohshima, Takashi Okiji

CLINICAL ORAL INVESTIGATIONS 18 ( 2 ) 625 - 634 2014年3月

What happens when you rinse? 査読

Takenaka S, Pitts B, Stewart PS

Cell (Journal's online) 2012年

Current and future strategies for the control of mature oral biofilms—Shift from a bacteria-targeting to a matrix-targeting approach. 査読

Takenaka S, Ohshima H, Ohsumi T, Okiji T

J Oral Biosci 54 ( 4 ) 173 - 179 2012年

Translucency and color change of tooth-colored temporary coating materials 査読

Shoji Takenaka, Rika Wakamatsu, Yumiko Ozoe, Fumihito Tomita, Masayoshi Fukushima, Takashi Okiji

AMERICAN JOURNAL OF DENTISTRY 22 ( 6 ) 361 - 365 2009年12月

Diffusion of Macromolecules in Model Oral Biofilms 査読

Shoji Takenaka, Betsey Pitts, Harsh M. Trivedi, Philip S. Stewart

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75 ( 6 ) 1750 - 1753 2009年3月

Impact of Streptococcus mutans on the generation of fluorescence from artificially induced enamel and dentin carious lesions in vitro 査読

Yoshimi Shigetani, Shoji Takenaka, Akira Okamoto, Neamat Abu-Bakr, Masaaki Iwaku, Takashi Okiji

ODONTOLOGY 96 ( 1 ) 21 - 25 2008年7月

Direct visualization of spatial and temporal patterns of antimicrobial action within model oral biofilms 査読

Shoji Takenaka, Harsh M. Trivedi, Audrey Corbin, Betsey Pitts, Philip S. Stewart

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 74 ( 6 ) 1869 - 1875 2008年3月

Removal of resin-based root canal filling materials with K3 rotary instruments: Relative efficacy for different combinations of filling materials 査読

Naoyuki Iizuka, Shoji Takenaka, Yoshimi Shigetani, Takashi Okiji

DENTAL MATERIALS JOURNAL 27 ( 1 ) 75 - 80 2008年1月

Aerotolerance of Peptostreptococcus anaerobius biofilms 査読

Takashi Fukuda, Shoji Takenaka, Kohichi Kota, Masaaki Iwaku, Etsuro Hoshino

Microbial Ecology in Health and Disease 16 ( 4 ) 205 - 210 2004年12月

Bactericidal effects of different root canal cleaning methods on the microroganisms in the deep layers of root canal dentine. 査読

Kota K, Takenaka S, Kashiwada T, Iwaku M

Niigata Dent J 32 ( 2 ) 251 - 255 2002年10月

Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S-mutans biofilm 査読

M Zhu, S Takenaka, M Sato, E Hoshino

ORAL MICROBIOLOGY AND IMMUNOLOGY 16 ( 1 ) 54 - 56 2001年2月

Influence of starvation and biofilm formation on acid resistance of Streptococcus mutans 査読

M Zhu, S Takenaka, M Sato, E Hoshino

ORAL MICROBIOLOGY AND IMMUNOLOGY 16 ( 1 ) 24 - 27 2001年2月

Artificial Pseudomonas aeruginosa biofilms and confocal laser scanning microscopic analysis 査読

S. Takenaka, M. Iwaku, E. Hoshino

Journal of Infection and Chemotherapy 7 ( 2 ) 87 - 93 2001年

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