研究者詳細 - 竹中　彰治

論文 - 竹中　彰治

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Hyperglycemia in obese type 2 diabetes mellitus rats affects pulp wound healing

Rosa Baldeon-Gutierrez, Naoto Ohkura, Nagako Yoshiba, Shintaro Takahara, Susan Gomez-Kasimoto, Takako Ida, Naoki Edanami, Kunihiko Yoshiba, Shoji Takenaka, Yuichiro Noiri

JOURNAL OF ORAL BIOSCIENCES 67 ( 4 ) 2025年12月

異種歯乳頭由来幹細胞を用いた再生歯内療法における有用性の評価

高原信太郎, 大倉直人, Baldeon-Gutierrez Rosa, Gomez-Kasimoto Susan, Thongtade Pemika, 竹中彰治, 廣瀬陽菜, 吉羽永子, 松崎英津子, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 163回 79 - 79 2025年10月

Role of Dystrophic Calcification in Reparative Dentinogenesis After Rat Molar Pulpotomy. 国際誌

Naoki Edanami, Kunihiko Yoshiba, Razi Saifullah Ibn Belal, Nagako Yoshiba, Shoji Takenaka, Naoto Ohkura, Shintaro Takahara, Takako Ida, Rosa Baldeon, Susan Kasimoto, Pemika Thongtade, Yuichiro Noiri

International journal of molecular sciences 26 ( 15 ) 2025年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

Vital pulp therapy with calcium hydroxide or mineral trioxide aggregate (MTA) rapidly induces dystrophic calcification and promotes the accumulation of two members of small integrin-binding ligand N-linked glycoproteins: osteopontin (OPN) and dentin matrix protein-1 (DMP1). However, the precise relationship between these initial events and their roles in reparative dentinogenesis remain unclear. This study aimed to clarify the relationship between dystrophic calcification, OPN and DMP1 accumulation, and reparative dentin formation. Pulpotomy was performed on rat molars using MTA or zirconium oxide (ZrO2). ZrO2 was used as a control to assess pulp healing in the absence of dystrophic calcification. Pulpal responses were evaluated from 3 h to 7 days postoperatively via elemental mapping, micro-Raman spectroscopy, and histological staining. In the MTA-treated group, a calcium-rich dystrophic calcification zone containing calcite and hydroxyapatite was observed at 3 h after treatment; OPN and DMP1 accumulated under the dystrophic calcification zone by day 3; reparative dentin formed below the region of OPN and DMP1 accumulation by day 7. In contrast, these reactions did not occur in the ZrO2-treated group. These results suggest that dystrophic calcification serves as a key trigger for OPN and DMP1 accumulation and plays a pivotal role in reparative dentinogenesis.

DOI： 10.3390/ijms26157130

PubMed

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歯根膜における5型コラーゲンの組織学的解析

井田貴子, 大倉直人, Gomez Kasimoto Susan Kiara, Baldeon-Gutierrez Rosa, 枝並直樹, 竹中彰治, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 162回 140 - 140 2025年6月

歯周病原細菌が産生する外膜小胞の血行性胎盤移行と胎盤栄養膜細胞への内在化

山本倫子, 大倉直人, 稲垣舞, 竹中彰治, 近藤もも香, 立川正憲

日本薬剤学会年会講演要旨集 40年会 133 - 133 2025年5月

Influence of Tooth Maturity on Healing Outcomes in Regenerative Endodontics

S. Takahara, N. Ohkura, N. Yoshiba, R. Baldeon-Gutierrez, S. Gomez-Kasimoto, N. Edanami, T. Ida, S. Takenaka, K. Yoshiba, Y. Noiri

Journal of Dental Research 2025年4月

掲載種別：研究論文（学術雑誌）出版者・発行元：SAGE Publications

Regenerative endodontic procedures using blood clots (BC-REP) for immature teeth typically exhibit a periodontal ligament–like healing pattern. However, a pulp-like healing pattern is observed in the presence of residual pulp. This study aimed to clarify the healing phenotype according to tooth maturity when performing BC-REP in the presence of residual pulp, focusing on migrated mesenchymal stem/stromal cells (MSCs). BC-REP rat molar models were created in the presence of residual pulp at ages corresponding to tooth developmental stages, from immature to mature (5 wk: immature, root is still growing; 8 wk: near mature, root has finished growing in length but the apex is not formed; 11 wk: mature, the apex is formed). The healing pattern and histological MSC markers (α–smooth muscle actin [α-SMA], CD73, CD90, and CD146) were investigated. The frequency of periodontal ligament–like healing was higher in mature teeth than in immature teeth. In addition, more healing macrophages were observed at the apical site 28 d after BC-REP, which is the final stage of healing. In immature teeth, double-immunopositive cells for proliferation markers (ki67 and proliferating cell nuclear antigen [PCNA]) and α-SMA were frequently observed in the vicinity of the root canal orifice 7 d after treatment, which is the early stage of healing. By contrast, in mature teeth, the number of CD73-, CD90-, and CD146-immunopositive cells increased at the apical site after 7 and 28 d. CD90- and CD146-immunopositive cells expressed cell proliferation markers (ki67 or PCNA) after 7 d. MSC migration after BC-REP likely varies based on tooth maturity, resulting in different healing phenotypes.

DOI： 10.1177/00220345251325826

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その他リンク： https://journals.sagepub.com/doi/full-xml/10.1177/00220345251325826

ヒト血液脳関門を模倣したマイクロ流体モデルにおける歯周病原性細菌由来ナノ粒子の取り込み動態

近藤もも香, 万々桜, 大倉直人, 竹中彰治, 梶弘和, 稲垣舞, 立川正憲

生体医歯工学共同研究拠点成果報告書 2024年度 61 - 61 2025年4月

An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars. 国際誌

Shintaro Takahara, Naoki Edanami, Razi Saifullah Ibn Belal, Kunihiko Yoshiba, Shoji Takenaka, Naoto Ohkura, Nagako Yoshiba, Susan Gomez-Kasimoto, Yuichiro Noiri

Journal of functional biomaterials 16 ( 1 ) 2025年1月

記述言語：英語掲載種別：研究論文（学術雑誌）

This study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) and Nishika Canal Sealer BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial root canals of the upper first molars were pulpectomized and overfilled with EBC, NBG, or, as reference, epoxy resin-based AH Plus (AHP). After 28 days, periapical tissue reactions were assessed using microcomputed tomography and histological staining. The elemental composition and chemical composition of the extruded EBC and NBG were analyzed at Day 1 and 28 using an electron probe microanalyzer and micro-Raman spectroscopy. No periapical lesions were observed with the sealer extrusion. Additionally, inflammation around the extruded EBC and NBG was minimal to mild on Day 28, whereas moderate inflammation was found around the extruded AHP. Silicon concentration in the extruded EBC and NBG decreased significantly from Day 1 to 28, with almost no silicon present on Day 28. Furthermore, the extruded EBC and NBG became calcium- and phosphorus-rich, showing a Raman band for hydroxyapatite on Day 28. In conclusion, EBC and NBG demonstrated favorable biocompatibility and the ability to release silicon elements and produce hydroxyapatite when extruded into the periapical tissues. AHP showed moderate periapical tissue irritancy.

DOI： 10.3390/jfb16010014

PubMed

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Interaction between the human dental microbiome and host gingival model

Maki Sotozono, Ryouhei Takahashi, Takako Ida, Ryoko Nagata, Rui Saito, Niraya Kornsombut, Jutharat Manuschai, Shoji Takenaka, Yuichiro Noiri

Microbiota and Host 3 ( 1 ) 2024年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：Bioscientifica

Objective

Interactions between the dental biofilm and host are important to study infectious oral diseases such as dental caries and periodontitis. Previous studies have reported the reaction of a host gingival model cocultured with bacteria or biofilms. However, these studies only report results of a gingival model, and a biofilm cocultured with a gingival model has never been investigated, although the microbiome of dental biofilms is associated with the maintenance of health and the onset of infectious oral diseases. Therefore, we aimed to investigate the reaction of a gingival model and experimental biofilm. An in situ dental biofilm model, which enables biofilm formation in the oral cavity, was used to prepare experimental biofilms.

Methods

The in situ biofilm was cocultured with a reconstructed human gingival (RHG) model. We performed 16S rRNA sequence analysis, metagenomic function prediction analysis, total bacterial cell counts using quantitative polymerase chain reaction and confocal laser microscopy observation in addition to gene expression and histological investigation of RHG.

Results

We found a change in Veillonella abundance in the in situ biofilms cocultured with RHG. Metagenomic function prediction analysis revealed that the activity of certain pathways associated with amino acid production, lactic acid fermentation and the glycolysis system significantly increased in biofilms cocultured with RHG.

Conclusion

The microbiome and functions of dental biofilms can be affected by host gingival tissues. Our findings will contribute to the establishment of a host–biofilm interaction model to understand the pathophysiology of oral infectious diseases.

DOI： 10.1530/mah-24-0008

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その他リンク： https://mah.bioscientifica.com/downloadpdf/journals/mah/3/1/MAH-24-0008.xml

各種受容細胞におけるP.gingivalis由来外膜小胞の取り込み動態解析

大倉直人, 吉羽永子, 立川正憲, 中馬吉郎, Baldeon Gutierrez Rosa Edith, 高原信太郎, Gomez Kasimoto Susan, 井田貴子, 枝並直樹, 竹中彰治, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 161回 150 - 150 2024年11月

Effects of Tooth Desensitizers on Streptococcus mutans Biofilm Formation Using a Modified Robbins Device Flow Cell System

Niraya Kornsombut, Shoji Takenaka, Jutharat Manuschai, Maki Sotozono, Ryoko Nagata, Takako Ida, Risako Sato, Rui Saito, Ryouhei Takahashi, Daichi Sato, Yuichiro Noiri

International Journal of Molecular Sciences 25 ( 19 ) 10703 - 10703 2024年10月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study aimed to assess the antibiofilm effects of dentin desensitizers using a modified Robbins device flow cell system. The test desensitizers were Saforide, Caredyne Shield, and Clinpro White Varnish. Standardized dentin specimens were prepared from human single-rooted premolars, treated with one of the materials, and mounted on the modified Robbins device flow cell system. Streptococcus mutans biofilms were developed for 24 h at 37 °C under anaerobic conditions. Scanning electron microscopy, fluorescence confocal laser scanning microscopy, viable and total cell counts, acid production, and gene expression analyses were performed. A wavelength-dispersive X-ray spectroscopy electron probe microanalyzer was used to analyze the ion incorporations. Clinpro White Varnish showed the greatest inhibition, suggesting its suppression of bacterial adherence and transcription of genes related to biofilm formation. Saforide reduced only the number of viable bacteria, but other results showed no significant difference. The antibiofilm effects of Caredyne Shield were limited. The uptake of ions released from a material into dentin varies depending on the element. Clinpro White Varnish is effective for the short-term treatment of tooth sensitivity due to dentin demineralization. It prioritizes remineralization by supplying calcium and fluoride ions while resisting biofilm formation.

DOI： 10.3390/ijms251910703

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In Vitro Inhibitory Effect of Silver Diamine Fluoride Combined with Potassium Iodide against Mixed-Species Biofilm Formation on Human Root Dentin 査読

Manuschai J., Sotozono M., Takenaka S., Kornsombut N., Takahashi R., Saito R., Nagata R., Ida T., Noiri Y.

Antibiotics 13 ( 8 ) 2024年8月

歯髄創傷治癒および歯髄再生過程におけるリン酸トランスポーター(Pit-1)の免疫組織学的解析

大倉直人, Baldeon Gutierrez Rosa Edith, 高原信太郎, Gomez Kasimoto Susan Kiara, 枝並直樹, 井田貴子, 外園真規, 永田量子, 竹中彰治, 吉羽邦彦, 吉羽永子, 野杁由一郎

日本歯科保存学雑誌 67 ( 3 ) 165 - 173 2024年6月

A novel 12-membered ring non-antibiotic macrolide EM982 attenuates cytokine production by inhibiting IKKβ and IκBα phosphorylation

Rui Saito, Hisanori Domon, Takumi Hiyoshi, Satoru Hirayama, Tomoki Maekawa, Shoji Takenaka, Yuichiro Noiri, Akari Ikeda, Tomoyasu Hirose, Toshiaki Sunazuka, Yutaka Terao

Journal of Biological Chemistry 107384 - 107384 2024年5月

In situデンタルバイオフィルムモデルを用いた宿主バイオフィルム間相互作用の解明

高橋竜平, 外園真規, 井田貴子, 永田量子, Kornsombut Niraya, 齋藤瑠郁, 竹中彰治, 野杁由一郎

BACTERIAL ADHERENCE & BIOFILM 37 35 - 41 2024年5月

Antibiofilm Properties and Demineralization Suppression in Early Enamel Lesions Using Dental Coating Materials 査読

Niraya Kornsombut, Shoji Takenaka, Maki Sotozono, Ryoko Nagata, Takako Ida, Jutharat Manuschai, Rui Saito, Ryouhei Takahashi, Yuichiro Noiri

Antibiotics 13 106 2024年1月

担当区分：責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

This study aimed to investigate the effects of dental coating materials on Streptococcus mutans biofilm formation. The test materials were PRG Barrier Coat (PRG), BioCoat Ca (BioC), and FluorDental Jelly (FluorJ). Bovine enamel specimens were demineralized to mimic early enamel lesions. The biofilm was developed on a specimen treated with one of the materials by using a modified Robbins device flow-cell system. Scanning electron and fluorescence confocal laser scanning microscopy, viable and total cell counts, and gene expression assessments of the antibiofilm were performed. Ion incorporation was analyzed using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer. All materials allowed biofilm formation but reduced its volume. FluorJ was the only material that inhibited biofilm accumulation and had a bactericidal effect, revealing 0.66 log CFU in viable cells and 1.23 log copy reduction in total cells compared with the untreated group after 24 h of incubation. The ions released from PRG varied depending on the element. BioC contributed to enamel remineralization by supplying calcium ions while blocking the acid produced from the biofilm. In summary, the dental coating materials physically prevented acid attacks from the biofilm while providing ions to the enamel to improve its mechanical properties.

DOI： 10.3390/antibiotics13010106

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再生歯内療法後の治癒過程初期における各種間葉系幹細胞マーカー陽性細胞の局在性

高原信太郎, 大倉直人, 吉羽永子, 竹中彰治, 枝並直樹, 吉羽邦彦, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 159回 47 - 47 2023年10月

歯根形成時におけるTissue nonspecific alkaline phosphataseの機能解析

大倉直人, 吉羽永子, 高原信太郎, Baldeon Gutierrez Rosa Edith, Gomez-Kasimoto Susan, 井田貴子, 枝並直樹, 竹中彰治, 吉羽邦彦, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 159回 92 - 92 2023年10月

Preparing of Point-of-Care Reagents for Risk Assessment in the Elderly at Home by a Home-Visit Nurse and Verification of Their Analytical Accuracy 査読

Shoji Takenaka, Hiroshi Moro, Utako Shimizu, Takeshi Koizumi, Kei Nagano, Naoki Edanami, Naoto Ohkura, Hisanori Domon, Yutaka Terao, Yuichiro Noiri

Diagnistics ahead of online ( 14 ) 2407 - 2407 2023年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

With the rising number of older adults residing at home, there is a growing need for risk assessment and patient management in home nursing. This study aims to develop point-of-care test (POCT) reagents that can aid in risk assessment and home care, especially in settings with limited resources. Our focus was on creating a C-reactive protein (CRP) POCT, which can accurately diagnose clinically significant judgment values in home nursing. Additionally, we assessed the utility of the HemoCue WBC DIFF system in providing differential counts of white blood cells (WBC). These performances were compared with a laboratory test using blood samples from patients with pneumonia. The CRP POCT showed a comparable result to that of a laboratory method, with an average kappa index of 0.883. The leukocyte count showed good agreement with the reference method. While the correlation coefficients for both neutrophil and lymphocyte counts were deemed acceptable, it was observed that the measured values tended to be smaller in cases where the cell count was higher. This proportional error indicates a weak correlation with the neutrophil-to-lymphocyte ratio. CRP POCT and WBC counts provided reliable and accurate judgments. These tools may benefit risk management for older adults at home, patients with dementia who cannot communicate, and those living in depopulated areas.

DOI： 10.3390/diagnostics13142407

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歯髄再生療法モデルラットを用いた歯根成長段階による治癒形態の比較解析

高原信太郎, 大倉直人, 吉羽邦彦, 吉羽永子, 竹中彰治, 枝並直樹, 庭野和明, 野杁由一郎

特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集 158回 31 - 31 2023年5月

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