Updated on 2025/10/09

写真a

 
NAKANO Masaru
 
Organization
Academic Assembly Institute of Science and Technology NOUGAKU KEIRETSU Professor
Faculty of Agriculture Department of Agriculture Professor
Title
Professor
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Degree

  • 学術博士 ( 1993.3   千葉大学 )

Research Areas

  • Environmental Science/Agriculture Science / Horticultural science

Research History (researchmap)

  • Niigata University   Faculty of Agriculture, Department of Agriculture   Professor

    2017.4

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  • Niigata University   Faculty of Agriculture Department of Agrobiology   Associate Professor

    2004.4

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  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4

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  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1996.4 - 1997.3

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  • Niigata University   Graduate School of Science and Technology   Assistant

    1994.4 - 1996.3

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Research History

  • Niigata University   Faculty of Agriculture Department of Agriculture   Professor

    2017.4

  • Niigata University   College of Creative Studies   Professor

    2017.4

  • Niigata University   Graduate School of Science and Technology Life and Food Sciences   Associate Professor

    2004.4 - 2017.3

  • Niigata University   Faculty of Agriculture Department of Agrobiology   Associate Professor

    2004.4 - 2017.3

  • Niigata University   Faculty of Agriculture   Associate Professor (as old post name)

    1996.4 - 1997.3

  • Niigata University   Graduate School of Science and Technology   Research Assistant

    1994.4 - 1996.3

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Papers

  • Induction of dwarf and early flowering phenotypes in Tricyrtis sp. by ectopic expression of LEAFY from Arabidopsis thaliana

    Darunmas Sankhuan, Meiqiao Ji, Sota Takanashi, Yuto Imamura, Shoichi Sato, Kanyaratt Supaibulwatana, Masahiro Otani, Masaru Nakano

    PLANT BIOTECHNOLOGY   39 ( 2 )   205 - 208   2022.6

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    DOI: 10.5511/plantbiotechnology.22.0118a

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  • Production of colored foliage phenotypes in Kalanchoe blossfeldiana by ectopic expression of R2R3 MYB genes

    Takuo Fujimoto, Masahiro Otani, Masaru Nakano

    JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY   2022.1

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    DOI: 10.1007/s13562-021-00760-3

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  • Suppression of B function by chimeric repressor gene-silencing technology (CRES-T) reduces the petaloid tepal identity in transgenic Lilium sp.

    Masahiro Otani, Kaiki Aoyagi, Masaru Nakano

    PLOS ONE   15 ( 8 )   e0237176 - e0237176   2020.8

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0237176

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  • Plant regeneration and <i>Agrobacterium</i>-mediated genetic transformation systems in liliaceous ornamental plants Reviewed

    Nakano Masaru, Otani Masahiro

    Plant Biotechnology   2020

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    Language:English   Publisher:Japanese Society for Plant Cell and Molecular Biology  

    <p>The family Liliaceae (Cronquist system) contains various important ornamental plants. We have been examining for about 20 years the establishment of plant regeneration and genetic transformation systems in liliaceous ornamental plants for their biotechnological breeding and elucidation of the molecular mechanisms determining ornamental traits. In this review, studies on <i>in vitro</i> plant regeneration in 7 genera and on <i>Agrobacterium</i>-mediated production of transgenic plants in 4 genera are described. Plant regeneration was achieved via callus cultures in <i>Agapanthus</i>, <i>Hemerocallis</i>, <i>Hosta</i>, <i>Lilium</i>, <i>Muscari</i> and <i>Tricyrtis</i>. Auxins (2,4-dichrolophenoxyacetic acid, α-naphthaleneacetic acid and/or picloram) were effective for inducing regenerable calli. <i>Tulipa</i> species and cultivars were very recalcitrant to callus induction and plant regeneration. <i>Agrobacterium</i>-mediated transformation was examined in <i>Agapanthus</i>, <i>Lilium</i>, <i>Muscari</i> and <i>Tricyrtis</i>, and transgenic plants were obtained in all genera by using regenerable calli as a target material for <i>Agrobacterium</i> inoculation, inoculation and co-cultivation with <i>Agrobacterium</i> in the presence of acetosyringone, and selection of transgenic tissues and plantlets on hygromycin-containing media. Among 4 genera, <i>Tricyrtis</i> has several advantages for transformation studies: higher transformation efficiency, relatively small plant size, ease of cultivation, and taking only 1 year from <i>in vitro</i> regeneration to flowering. We are now investigating the molecular mechanisms for determining plant form, flower color and flower form by using <i>Tricyrtis</i> spp. as liliaceous model plants.</p>

    DOI: 10.5511/plantbiotechnology.20.0114a

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  • Production and characterization of intersectional hybrids between Tricyrtis sect. Brachycyrtis and sect. Hirtae via ovule culture Reviewed

    Toshiya Inamura, Manami Nakazawa, Mitsuyo Ishibe, Masahiro Otani, Masaru Nakano

    Plant Biotechnology   36 ( 3 )   175 - 180   2019.9

  • Production and characterization of wide hybrid plants in the genus Tricyrtis using transgenic plants carrying the gibberellin 2-oxidase gene from Torenia fournieri. Reviewed

    Masahiro Otani, Shoichi Sato, Mitsuyo Ishibe, Masaru Nakano

    Scientia Horticulturae   254   215 - 221   2019.5

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    DOI: 10.1016/j.scienta.2019.05.003

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  • Leaf color modification in Torenia concolor by ectopic expression of R2R3-MYB genes

    Takuo Fujimoto, Miho Takano, Masahiro Otani, Masaru Nakano

    Memoirs of the Faculty of Agriculture, Niigata University   71   35 - 42   2019.2

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

    Ornamental plants with red, purple and/or yellow leaves are generally called colored-leaf plants. Colored leaves are resulted from biosynthesis and accumulation of pigments in leaf cells. Among such pigments, anthocyanins are the most major ones, and they are synthesized through the flavonoid biosynthetic pathway. R2R3-MYB, a member of the MYB transcription factor family, is known to activate the flavonoid biosynthetic pathway. In the present study, we examined genetic transformation of Torenia concolor with R2R3-MYB genes from Tricyrtis sp. (TrMYB1) or Arabidopsis thaliana (PAP1) for producing novel colored-leaf plants. Totally ten and eight independent transgenic plants have so far been obtained by transformation with TrMYB1 and PAP1, respectively. Transgenic plants could be classified into three types according to the leaf color phenotype: green leaves as the wild-type plants (Type I), light red-purple leaves (Type II), and deep red-purple leaves (Type III). Three Type I, two Type II and five Type III transgenic plants were obtained for TrMYB1, whereas two Type I, three Type II and three Type III transgenic plants were obtained for PAP1. Total anthocyanin contents in leaves of Type III transgenic plants were significantly higher than the vector control. Real-time RT-PCR analysis showed that TrMYB1 and PAP1 expression levels generally correlated with the degree of leaf color alteration. These results indicate that ectopic expression of the heterogeneous R2R3-MYB genes in T. concolor may activate the flavonoid biosynthetic pathway in leaves leading to anthocyanin accumulation and leaf color alteration.

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    Other Link: http://hdl.handle.net/10191/50915

  • Isolation and functional analysis of FLOWERING LOCUS T homologous gene from Vanda hybrid. Reviewed

    Kanokwan Panjama, Eriko Suzuki, Masahiro Otani, Masaru Nakano, Norikuni Ohtake, Takuji Ohyama, Weenun Bundithya, Kuni Sueyoshi, Soraya Ruamrungsri

    Journal of Plant Biochemistry and Biotechnology   2019.1

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    DOI: 10.1007/s13562-019-00487-2

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  • Expression and Functional Analyses of Five B-class Genes in Grape Hyacinth (Muscari armeniacum) Reviewed

    Miura Kana, Nakada Mutsumi, Kubota Shosei, Sato Shusei, Nagano Soichiro, Kobayashi Akie, Teranishi Mika, Nakano Masaru, Kanno Akira

    THE HORTICULTURE JOURNAL   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2503/hortj.UTD-036

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  • Ectopic expression of the R2R3-MYB gene from Tricyrtis sp. results in leaf color alteration in transgenic Pelargonium crispum Reviewed

    Atsushi Kanemaki, Masahiro Otani, Miho Takano, Takuo Fujimoto, Hiroaki Okuhara, Toshikazu Nomizu, Masayoshi Kondo, Hitoshi Kobayashi, Fumi Tatsuzawa, Masaru Nakano

    Scientia Horticulturae   240   411 - 416   2018.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier B.V.  

    DOI: 10.1016/j.scienta.2018.06.029

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  • Comprehensive isolation and expression analysis of the flavonoid biosynthesis-related genes in Tricyrtis spp. Reviewed

    NAKANO Masaru

    Biologia Plantarum   62 ( 4 )   684 - 692   2018.9

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  • Chromosome doubling of Tricyrtis formosana by in vitro spindle toxin treatments

    Masaru Nakano, Chiaki Goto, Miki Yamakawa, Mitsuyo Ishibe, Toshiya Inamura, Masahiro Otani

    Memoirs of the Faculty of Agriculture, Niigata University   70   9 - 13   2018.2

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  • Erratum to: Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua L. (Plant Cell, Tissue and Organ Culture (PCTOC), (2017), 129, 2, (223-236), 10.1007/s11240-017-1171-1) Reviewed

    Phithak Inthima, Masaru Nakano, Masahiro Otani, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka, Kanyaratt Supaibulwatana

    Plant Cell, Tissue and Organ Culture   129 ( 2 )   223 - 236   2017.9

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    DOI: 10.1007/s11240-017-1171-1

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  • Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid of metabolites Artemisia annua L. (vol 129, pg 223, 2017) Reviewed

    Phithak Inthima, Masaru Nakano, Masahiro Otani, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka, Kanyaratt Supaibulwatana

    PLANT CELL TISSUE AND ORGAN CULTURE   130 ( 3 )   689 - 689   2017.9

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  • Corrigendum: Suppression of B function strongly supports the modified ABCE model in Tricyrtis sp. (Liliaceae) (Scientific Reports (2017) 6 (24549) DOI: 10.1038/srep24549) Reviewed

    Masahiro Otani, Ahmad Sharifi, Shosei Kubota, Kanako Oizumi, Fumi Uetake, Masayo Hirai, Yoichiro Hoshino, Akira Kanno, Masaru Nakano

    Scientific Reports   6   24549   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Publishing Group  

    DOI: 10.1038/srep24549

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    Other Link: http://orcid.org/0000-0002-9762-4842

  • Suppression of B function strongly supports the modified ABCE model in Tricyrtis sp. (Liliaceae) (vol 6, 24549, 2016) Reviewed

    Masahiro Otani, Ahmad Sharifi, Shosei Kubota, Kanako Oizumi, Fumi Uetake, Masayo Hirai, Yoichiro Hoshino, Akira Kanno, Masaru Nakano

    SCIENTIFIC REPORTS   7   42322   2017.2

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  • Chromosome doubling of a colchicaceous intergeneric hybrid by spindle toxin treatments

    Yamakawa Miki, Kishimoto Tomonari, Sato Takeru, Saito Tomoka, Amano Junji, Otani Masahiro, Nakano Masaru

    新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University   68   25 - 30   2016.2

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    The family Colchicaceae contains some important ornamental plants, such as Gloriosa spp. and Sandersonia aurantiaca. We have produced various intergeneric hybrid plants in this family in order to obtain wide variability in horticultural traits and to develop novel cultivars. In the present study, we examined chromosome doubling of a diploid intergeneric hybrid between L. modesta and G. superba &#039;Lutea&#039; (Lit×Gsu-1; 2n=2x=22) for fertility restoration and for further widening the variability. Shoot apical segments harvested from in vitro shoot cultures were treated with various concentrations of amiprophos-methyl (APM; 10, 20 or 40 mg L^&lt;-1&gt;), colchicine (COL; 100, 500 or 1000 mg L^&lt;-1&gt;) or oryzalin (ORY; 5, 10 or 20 mg L^&lt;-1&gt;) for 24 h. Two months after spindle toxin treatment, the highest percentage apical segments with shoot elongation (80.0%) was obtained in 10 mg L^&lt;-1&gt; APM treatment. Flow cytometry analysis of elongated shoots showed that a solid tetraploid (4x) was obtained from 40 mg L^&lt;-1&gt; APM treatment and ploidy chimeras (2x+4x) were obtained from treatments with 10 or 20 mg L^&lt;-1&gt; APM and 5 or 10 mg L^&lt;-1&gt; ORY. Tetraploid and polyploidy chimera shoots showed compact forms

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  • Molecular approaches to flower breeding

    Nakano Masaru, Mii Masahiro, Kobayashi Hitoshi, Otani Masahiro, Yagi Masafumi

    Breeding Research   18 ( 1 )   34 - 40   2016

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    Language:Japanese   Publisher:Japanese Society of Breeding  

    DOI: 10.1270/jsbbr.18.34

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  • Improvement of Vase Life in Cut Tulips by Pretreatments with 2-Chloroethylphosphonic Acid and 6-Benzylaminopurine

    Watanabe Yusuke, Miyajima Kazunori, Nakano Masaru, Ichimura Kazuo

    Horticultural Research (Japan)   15 ( 4 )   445 - 452   2016

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    Language:Japanese   Publisher:一般社団法人 園芸学会  

    &lt;p&gt;Ornamental values of cut tulip (&lt;i&gt;Tulipa gesneriana&lt;/i&gt; L.) flowers are reduced by elongation of the flower stalks and leaf yellowing. The effect of pretreatments with 2-chloroethylphosphonic acid (CEPA), 6-benzylaminopurine (BA), and gibberellic acid (GA&lt;sub&gt;3&lt;/sub&gt;) at various concentrations on the vase life of cut &#039;Christmas Dream&#039; flowers was investigated. Pretreatments with CEPA at 25, 50, and 100 mg・L&lt;sup&gt;–1&lt;/sup&gt; significantly suppressed flower stalk elongation, but shortened the flower longevity by 2 days. Pretreatments with BA at 5, 25, and 125 mg・L&lt;sup&gt;–1&lt;/sup&gt; suppressed leaf yellowing, but pretreatment with GA&lt;sub&gt;3&lt;/sub&gt; suppressed leaf yellowing only slightly. Flower longevity was not affected by pretreatments with BA or GA&lt;sub&gt;3&lt;/sub&gt;. A combined pretreatment with 50 mg・L&lt;sup&gt;–1&lt;/sup&gt; CEPA and 25 mg・L&lt;sup&gt;–1&lt;/sup&gt; BA suppressed flower stalk elongation and leaf yellowing in cut &#039;Christmas Dream&#039; flowers. Similar results were obtained from 7 cultivars of cut flowers. These results indicate that combined pretreatment with CEPA and BA is effective for suppressing flower stalk elongation and leaf yellowing of cut tulip flowers.&lt;/p&gt;

    DOI: 10.2503/hrj.15.445

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  • Double flower formation in Tricyrtis macranthopsis is related to low expression of AGAMOUS ortholog gene Reviewed

    Ahmad Sharifi, Kanako Oizumi, Shosei Kubota, Abdolreza Bagheri, Saeid Malekzadeh Shafaroudi, Masaru Nakano, Akira Kanno

    SCIENTIA HORTICULTURAE   193   337 - 345   2015.9

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    DOI: 10.1016/j.scienta.2015.06.050

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  • 'Niigata S3' is a new strawberry cultivar suitable for forcing culture under low temperature and insolation conditions Reviewed

    Naonori Hamato, Osamu Kotake, Nagaaki Ono, Hiroshi Kurashima, Masaru Nakano, Yuzuri Iwamoto, Yoshihiko Takahashi

    BREEDING SCIENCE   64 ( 4 )   427 - 434   2014.12

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    DOI: 10.1270/jsbbs.64.427

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  • Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH) Reviewed

    Tomonari Kishimoto, Miki Yamakawa, Daisuke Nakazawa, Junji Amano, Sachiko Kuwayama, Masaru Nakano

    EUPHYTICA   200 ( 2 )   251 - 257   2014.11

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    DOI: 10.1007/s10681-014-1152-y

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  • Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L. Reviewed

    Phithak Inthima, Masahiro Otani, Tomoya Hirano, Yoriko Hayashi, Tomoko Abe, Masaru Nakano, Kanyaratt Supaibulwatana

    PLANT CELL TISSUE AND ORGAN CULTURE   119 ( 1 )   131 - 139   2014.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11240-014-0519-z

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  • Characterization of CYCLOIDEA-like genes in controlling floral zygomorphy in the monocotyledon Alstroemeria Reviewed

    Yoichiro Hoshino, Toshiya Igarashi, Masumi Ohshima, Koichi Shinoda, Naho Murata, Akira Kanno, Masaru Nakano

    SCIENTIA HORTICULTURAE   169   6 - 13   2014.4

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    DOI: 10.1016/j.scienta.2014.01.046

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  • Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affecting plant morphology in transgenic Tricyrtis sp. Reviewed

    M. Otani, S. Meguro, H. Gondaira, M. Hayashi, M. Saito, D. S. Han, M. Nakano, S. Mori, T. Li, T. Niki, T. Nishijima, M. Koshioka

    Acta Horticulturae   1025   23 - 30   2014.3

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    Language:English   Publishing type:Research paper (international conference proceedings)   Publisher:International Society for Horticultural Science  

    DOI: 10.17660/ActaHortic.2014.1025.2

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  • Characterization of transgenic Agapanthus praecox ssp. orientalis plants ectopically expressing the class B MADS-box genes Reviewed

    N. Okuzumi, M. Otani, H. Otsubo, S. Meguro, Y. Hara, H. Umehara, M. Igarashi, D. S. Han, M. Nakano, Y. Hoshino, A. Kanno

    Acta Horticulturae   1025   93 - 98   2014.3

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    DOI: 10.17660/ActaHortic.2014.1025.14

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  • Isolation and characterization of the dihydroflavonol 4-reductase gene in the monocotyledonous ornamental Agapanthus praecox ssp orientalis (Leighton) Leighton Reviewed

    Shiro Mori, Masahiro Otani, Hitoshi Kobayashi, Masaru Nakano

    SCIENTIA HORTICULTURAE   166   24 - 30   2014.2

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    DOI: 10.1016/j.scienta.2013.12.009

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  • Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp carrying the gibberellin 2-oxidase gene Reviewed

    Masahiro Otani, Mitsuyo Ishibe, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka, Masaru Nakano

    PLANT BIOTECHNOLOGY   31 ( 4 )   335 - 340   2014

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    DOI: 10.5511/plantbiotechnology.14.0916a

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  • Horticultural Characterization of an Intergeneric Hybrid between a Septaploid Gloriosa Cultivar and Sandersonia aurantiaca Reviewed

    T. Kishimoto, D. Nakazawa, T. Sato, T. Saito, J. Amano, S. Kuwayama, D. S. Han, M. Nakano, H. Okuno, T. Godo, Y. Watanabe

    INTERNATIONAL SYMPOSIUM ON ORCHIDS AND ORNAMENTAL PLANTS   1025   51 - 56   2014

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  • Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp. Reviewed

    Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Yusuke Jikumaru, Yuji Kamiya, Tuoping Li, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka, Masaru Nakano

    Journal of Plant Physiology   170 ( 16 )   1416 - 1423   2013.11

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    DOI: 10.1016/j.jplph.2013.05.002

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  • Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii

    Masaru Nakano, Sachiko Kuwayama, Eriko Oka, Megumi Asano, Dong-Sheng Han, Toshinari Godo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   82 ( 1 )   57 - 62   2013.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2503/jjshs1.82.57

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  • Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii Reviewed

    Masaru Nakano, Sachiko Kuwayama, Eriko Oka, Megumi Asano, Dong-Sheng Han, Toshinari Godo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   82 ( 1 )   57 - 62   2013.1

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    DOI: 10.2503/jjshs1.82.57

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  • Effect of Sugar Treatments on the Vase Life of Cut Tulip (Tulipa gesneriana L.) Flowers

    渡邉祐輔, 宮島利功, 野水利和, 中野優, 市村一雄

    園芸学研究   12 ( 2 )   201-207 (J-STAGE) - 207   2013

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    Language:Japanese   Publisher:園芸学会  

    On the assumption that consumers treat cut flowers continuously with sugar, the effects of continuous treatments with glucose, sucrose, fructose, and trehalose at different concentrations were investigated on the vase life of cut tulip flowers. Continuous treatments with all 4 sugars extended the vase life of cut flowers of &amp;lsquo;Ile de France&amp;rsquo;. Continuous treatments with glucose, sucrose, and fructose were more effective than that with trehalose. However, all continuous treatments except for that with 1% glucose caused damage to leaves to various extents. Continuous treatment with 1% glucose increased the relative fresh weight of cut flowers in all 12 cultivars examined, and significantly extended the vase life of cut flowers in 9 cultivars. On the other hand, the effects of pulse treatments with glucose were also investigated based on the assumption that producers treat cut flowers for a short period with sugars before shipping. However, such pulse treatments had no effects on the vase life of cut flowers of &amp;lsquo;Ile de France&amp;rsquo;. Thus, continuous treatment with 1% glucose is effective for extending the vase life of tulip cut flowers.&lt;br&gt;

    DOI: 10.2503/hrj.12.201

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  • Decreased time from seed to flowering corm size in Zephyra elegans via in vitro cultivation Reviewed

    Alexis K. Vidal, Dong-Sheng Han, Masaru Nakano, Yoshiji Niimi

    CIENCIA E INVESTIGACION AGRARIA   39 ( 3 )   577 - 584   2012.9

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    DOI: 10.4067/S0718-16202012000300017

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  • Flower color alteration in the liliaceous ornamental Tricyrtis sp by RNA interference-mediated suppression of the chalcone synthase gene Reviewed

    Yukiko Kamiishi, Masahiro Otani, Hiroki Takagi, Dong-Sheng Han, Shiro Mori, Fumi Tatsuzawa, Hiroaki Okuhara, Hitoshi Kobayashi, Masaru Nakano

    MOLECULAR BREEDING   30 ( 2 )   671 - 680   2012.8

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    DOI: 10.1007/s11032-011-9653-z

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  • 培養物へのオリザリン処理による日本原産センノウ属植物の染色体倍加

    中野 優, 田崎 大夢, 野中 隆宏, 韓 東生, 神戸 敏成

    新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University   64 ( 2 )   101 - 105   2012.3

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    センノウ属(Lychnis spp. ;ナデシコ科)は約30種で構成され、そのうちの6種は日本に自生している。多くのセンノウ属植物は観賞的価値が高く、そのため鉢植え植物や庭植え植物として栽培されている。本研究では、園芸形質の拡大を目的として、日本原産の3種のセンノウ属植物、センジュガンピ(L. gracillima;2n=2x=24)、オグラセンノウ(L. kiusiana;2n=2x=24)およびフシグロセンノウ(L. miqueliana;2n=2x=24)について、培養物のオリザリン(ORY)処理による染色体倍加を試みた。これは、三倍体のセンノウ(L. senno)において最初に確立された方法である(Nonakaら、2011)。培養小植物体から調製した節切片を10mg L-1 ORYで24時間処理したところ、2ヵ月後にはいずれのセンノウ属植物においても70%以上の節切片が生存していた。また、ORY処理した節切片由来の小植物体についてフローサイトメトリー分析を行ったところ、センジュガンピ、オグラセンノウおよびフシグロセンノウの小植物体のそれぞれ15.7、15.1および7.8%が四倍

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  • Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae Reviewed

    Daisuke Nakazawa, Tomonari Kishimoto, Takeru Sato, Tomoka Saito, Junji Amano, Sachiko Kuwayama, Hajime Okuno, Toshinari Godo, Yusuke Watanabe, Dong-Sheng Han, Masaru Nakano

    EUPHYTICA   181 ( 2 )   197 - 202   2011.9

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  • Chromosome doubling of Lychnis spp. by in vitro spindle toxin treatment of nodal segments Reviewed

    Takahiro Nonaka, Eriko Oka, Megumi Asano, Sachiko Kuwayama, Hiromu Tasaki, Dong-Sheng Han, Toshinari Godo, Masaru Nakano

    SCIENTIA HORTICULTURAE   129 ( 4 )   832 - 839   2011.7

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  • Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder Reviewed

    Hiroki Takagi, Shintaro Sugawara, Tomoka Saito, Haruka Tasaki, Lu Yuanxue, Guan Kaiyun, Dong-Sheng Han, Toshinari Godo, Masaru Nakano

    PLANT BIOTECHNOLOGY REPORTS   5 ( 2 )   187 - 195   2011.4

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  • Promotion of somatic embryo production from embryogenic calluses of monocotyledonous and dicotyledonous plants by heavy-ion beam irradiation Reviewed

    Masaru Nakano, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Junji Amano, Dong-Sheng Han, Hiroyuki Saito, Hiromichi Ryuto, Nobuhisa Fukunishi, Tomoko Abe

    PLANT GROWTH REGULATION   60 ( 3 )   169 - 173   2010.4

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  • Property and Horticultural Use of Lychnis senno Conserved in Japan.

    神戸敏成, 中田政司, 中野優, 富田裕明, 三位正洋

    日本植物園協会誌   ( 44 )   197 - 204   2010.3

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  • Effect of picloram, an auxin-like plant growth regulator, on callus induction from mature seeds of Iris species native to Yunnan, China

    神戸 敏成, 中野 優, 沈 雲光

    Bulletin of the Botanic Gardens of Toyama   ( 15 )   17 - 21   2010.3

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  • Morphological variation in Tricyrtis hirta plants regenerated from heavy ion beam-irradiated embryogenic calluses Reviewed

    Masaru Nakano, Junji Amano, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Sachiko Kuwayama, Dong-Sheng Han, Hiroyuki Saito, Hiromichi Ryuto, Nobuhisa Fukunishi, Tomoko Abe

    PLANT BIOTECHNOLOGY   27 ( 2 )   155 - 160   2010

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  • ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE, AND C. SPECIOSA KURZ. Reviewed

    Masaru Nakano, Hiroki Takagi, Shintaro Sugawara, Tomoka Saito, Yusuke Watanabe, Lu Yuanxue, Guan Kaiyun, Toshinari Godo

    PROPAGATION OF ORNAMENTAL PLANTS   9 ( 4 )   216 - 222   2009.12

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  • Property and horticultural use of Lychnis senno conserved in Japan

    神戸 敏成, 中田 政司, 中野 優

    日本植物園協会誌   ( 44 )   197 - 204   2009

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  • Intergeneric hybridization among colchicaceous ornamentals, Gloriosa spp., Littonia modesta and Sandersonia aurantiaca via ovule culture Reviewed

    Junji Amano, Daisuke Nakazawa, Sachiko Kuwayama, Yoko Mizuta, Hajime Okuno, Yusuke Watanabe, Toshinari Godo, Dong-Sheng Han, Masaru Nakano

    PLANT BIOTECHNOLOGY   26 ( 5 )   535 - 541   2009

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  • Interspecific hybridization between triploid Senno (Lychnis senno Siebold et Zucc., Caryophyllaceae) and allied taxa of the genus Lychnis Reviewed

    Toshinari Godo, Junko Miyazaki, Sachiko Kuwayama, Shinjiro Ogita, Yasuo Kato, Masaru Nakano, Masashi Nakata

    PLANT BIOTECHNOLOGY   26 ( 3 )   301 - 305   2009

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  • Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan Preface Reviewed

    Masaru Nakano, Masahiro Mii

    PLANT BIOTECHNOLOGY   25 ( 6 )   509 - 509   2008.12

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  • Stability of β-glucuronidase gene expression in transgenic Tricyrtis hirta plants after two years of cultivation Reviewed

    S. Mori, E. Oka, H. Umehara, H. Kobayashi, Y. Hoshi, M. Kondo, K. Ogata, M. Nakano

    Biologia Plantarum   52 ( 3 )   513 - 516   2008.9

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    DOI: 10.1007/s10535-008-0099-z

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  • Morphological characterization of three intergeneric hybrids among Gloriosa superba 'Lutea', Littonia modesta, and Sandersonia aurantiaca (Colchicaceae) Reviewed

    Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Masaru Nakano, Toshinari Godo, Hajime Okuno

    HORTSCIENCE   43 ( 1 )   115 - 118   2008.2

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  • Flower form alteration by genetic transformation with the class B MADS-box genes of Agapanthus praecox spp. orientalis in transgenic dicot and monocot plants Reviewed

    Masaru Nakano, Hiroto Umehara, Yoshihiro Hara, Motohide Makino, Mika Igarashi, Mutsumi Nakada, Toru Nakamura, Yoichiro Hoshino, Akira Kanno

    MOLECULAR BREEDING   20 ( 4 )   425 - 429   2007.11

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  • Early identification of intra- and intergeneric hybrids among colchicaceous ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by flow cytometry and random amplified polymorphic DNA analyses Reviewed

    Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Tomo Oomiya, Toru Nakamura, Masaru Nakano

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   76 ( 1 )   73 - 78   2007.1

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  • Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus praecox ssp orientalis) plants at the flowering stage Reviewed

    Shiro Mori, Eriko Oka, Hiroto Umehara, Sakae Suzuki, Hitoshi Kobayashi, Yosuke Hoshi, Masayoshi Kondo, Yosuke Koike, Masaru Nakano

    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT   43 ( 1 )   79 - 87   2007.1

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  • Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, <i>Gloriosa</i> spp., <i>Littonia modesta</i> Hook. and <i>Sandersonia aurantiaca</i> Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

    Amano Junji, Kuwayama Sachiko, Mizuta Yoko, Oomiya Tomo, Nakamura Toru, Nakano Masaru

    J. Japan. Soc. Hort. Sci.   76 ( 1 )   73 - 78   2007

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    Plantlets derived from ovule culture following intra- or intergeneric crosses among Colchicaceous ornamentals, &lt;i&gt;Gloriosa&lt;/i&gt; spp., &lt;i&gt;Littonia modesta&lt;/i&gt; Hook. and &lt;i&gt;Sandersonia aurantiaca&lt;/i&gt; Hook., were subjected to flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses in order to verify their hybridity. For crosses between plants with apparently distinct relative fluorescence intensity (RFI) of nuclei, i.e., intrageneric crosses between &lt;i&gt;Gloriosa&lt;/i&gt; genotypes with different ploidy levels, and most intergeneric crosses, detection of hybrid plantlets could be readily accomplished by FCM analysis. The results of hybrid identification by RAPD analysis supported those of FCM analysis. In addition, RAPD analysis allowed the verification of the hybridity of intra- or intergeneric cross-derived plantlets, which could not be identified as hybrids by FCM analysis due to the similarity of RFI of the parents or appearance of the RFI peak in an unexpected position. Totally, 110 independent hybrid plantlets (60 intrageneric and 50 intergeneric hybrids) have so far been identified by FCM and/or RAPD analyses. Thus, FCM in combination with RAPD analyses offer simple a

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  • Somaclonal variation in Tricyrtis hirta plants regenerated from 1-year-old embryogenic callus cultures Reviewed

    Masaru Nakano, Toshikazu Nomizu, Keiko Mizunashi, Mami Suzuki, Shiro Mori, Sachiko Kuwayama, Megumi Hayashi, Hiroto Umehara, Eriko Oka, Hiroyuki Kobayashi, Megumi Asano, Shintaro Sugawara, Hiroki Takagi, Hiroyuki Saito, Masashi Nakata, Toshinari Godo, Yoshihiro Hara, Junji Amano

    SCIENTIA HORTICULTURAE   110 ( 4 )   366 - 371   2006.11

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    DOI: 10.1016/j.scienta.2006.07.026

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  • Production of inter-section hybrids between Primula filchnerae and P-sinensis through ovule culture Reviewed

    Junji Amano, Juntaro Kato, Masaru Nakano, Masahiro Mii

    SCIENTIA HORTICULTURAE   110 ( 2 )   223 - 227   2006.10

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    DOI: 10.1016/j.scienta.2006.06.027

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  • Callus formation and plant regeneration in various Lilium species and cultivars Reviewed

    Shiro Mori, Yukiko Adachi, Sakae Horimoto, Sakae Suzuki, Masaru Nakano

    In Vitro Cellular and Developmental Biology - Plant   41 ( 6 )   783 - 788   2005.11

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    DOI: 10.1079/IVP2005707

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  • The modified ABC model explains the development of the petaloid perianth of Agapanthus praecox ssp orientalis (Agapanthaceae) flowers Reviewed

    T Nakamura, T Fukuda, M Nakano, M Hasebe, T Kameya, A Kanno

    PLANT MOLECULAR BIOLOGY   58 ( 3 )   435 - 445   2005.6

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  • Comparison of Selection Efficiency between the bar and hpt Genes in Agrobacterium-mediated Transformation of Muscari armeniacum

    SUZUKI Sakae, NAKANO Masaru, KOIKE Yosuke, UEDA Kenji, INOUE Masayasu, NISHIHARA Masahiro, YAMAMURA Saburo

    J. Japan. Soc. Hort. Sci.   74 ( 1 )   60 - 62   2005.1

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    Embryogenic calli of &lt;i&gt;Muscari armeniacum&lt;/i&gt; cv. Blue Pearl were co-cultivated with &lt;i&gt;Agrobacterium tumefaciens&lt;/i&gt; EHA101/pBH, which harbored a binary vector that carries the phosphinothricin acetyltransferase (&lt;i&gt;bar&lt;/i&gt;) and hygromycin phosphotransferase (&lt;i&gt;hpt&lt;/i&gt;) genes. The calli were then transferred onto the selection media containing either 4 mg &amp;middot; liter&lt;sup&gt;&amp;minus;1&lt;/sup&gt; bialaphos or 75 mg &amp;middot; liter&lt;sup&gt;&amp;minus;1&lt;/sup&gt; hygromycin. Four to five weeks after transfer to the selection media, both bialaphos-resistant (Bia&lt;sup&gt;r&lt;/sup&gt;) and hygromycin-resistance (Hyg&lt;sup&gt;r&lt;/sup&gt;) cell clusters were produced. Over 90% of callus lines selected on a bialaphos-containing medium were verified to be transgenic by PCR analysis; this selection efficiency is comparable to that based on the &lt;i&gt;hpt&lt;/i&gt; gene. Leaf segments of plantlets regenerated from the transgenic Bia&lt;sup&gt;r&lt;/sup&gt; callus lines showed resistance to 4 mg &amp;middot; liter&lt;sup&gt;&amp;minus;1&lt;/sup&gt; of bialaphos, indicating that the &lt;i&gt;bar&lt;/i&gt; gene is useful not only as a selectable marker but also as a producer of herbicide-resistant transgenic plants of &lt;i&gt;M&lt;/i&gt;. &lt;i&gt;armeniacum&lt;/i&gt;.

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  • Comparison of selection efficiency between the bar and hpt genes in Agrobacterium mediated transformation of Muscari armeniacum Reviewed

    S Suzuki, M Nakano, Y Koike, K Ueda, M Inoue, M Nishihara, S Yamamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   74 ( 1 )   60 - 62   2005.1

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  • Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba Reviewed

    Masaru Nakano, Miho Nagai, Shigefumi Tanaka, Masashi Nakata, Toshinari Godo

    Plant Biotechnology   22 ( 3 )   221 - 224   2005

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    DOI: 10.5511/plantbiotechnology.22.221

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  • Agrobacterium-mediated production of transgenic plants in tricyrtis hirta (Liliaceae) Reviewed

    Y. Adachi, S. Mori, M. Nakano

    Acta Horticulturae   673   415 - 419   2005

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    DOI: 10.17660/ActaHortic.2005.673.52

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  • Isolation and characterization of a cytochrome P450 gene from Muscari armeniacum Reviewed

    S. Mori, M. Nakano, M. Kondo, Y. Hoshi, H. Kobayashi

    Acta Horticulturae   673   429 - 435   2005

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    DOI: 10.17660/ActaHortic.2005.673.54

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  • Agrobacterium-mediated transformation of Lilium longiflorum Reviewed

    Y. Hoshi, M. Kondo, H. Kobayashi, S. Mori, M. Nakano

    Acta Horticulturae   673   543 - 547   2005

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    DOI: 10.17660/ActaHortic.2005.673.73

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  • Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus Tricyrtis Reviewed

    Masaru Nakano, Keiko Mizunashi, Shigefumi Tanaka, Toshinari Godo, Masashi Nakata, Hiroyuki Saito

    In Vitro Cellular and Developmental Biology - Plant   40 ( 3 )   274 - 278   2004.5

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  • Somatic embryogenesis from leaf- and flower bud- derived calluses in various Muscari species and cultivars. Reviewed

    MORI Shiro, NAKANO Masaru

    Propagation of Ornamental Plants   4   58 - 62   2004.4

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  • Agrobacterium-mediated Transformation of Lettuce Widely Cultivated in Japan

    NAKANO Masaru, MAKINO Motohide, SUEYOSHI Kuni

    Bulletin of the Faculty of Agriculture, Niigata University   56 ( 2 )   59 - 66   2004.3

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  • Heterologous expression of the flavonoid 3′,5′-hydroxylase gene of Vinca major alters flower color in transgenic Petunia hybrida Reviewed

    S. Mori, H. Kobayashi, Y. Hoshi, M. Kondo, M. Nakano

    Plant Cell Reports   22 ( 6 )   415 - 421   2004.1

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    DOI: 10.1007/s00299-003-0709-3

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  • Production of transgenic lily plants by Agrobacterium-mediated transformation Reviewed

    Y. Hoshi, M. Kondo, S. Mori, Y. Adachi, M. Nakano, H. Kobayashi

    Plant Cell Reports   22 ( 6 )   359 - 364   2004.1

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  • Horticultural characterization of Angelonia salicariifolia plants transformed with wild-type strains of Agrobacterium rhizogenes Reviewed

    Y Koike, Y Hoshino, M Mii, M Nakano

    PLANT CELL REPORTS   21 ( 10 )   981 - 987   2003.6

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  • Retention property and Mass Spectrum of Labeled Standards of Cytokinins

    児島清秀, 谷沢貞幸, 高橋みや子, 塩田望, 馬場正, 池田富喜夫, 中野優, HAN D‐S, 新美芳二

    新潟大学農学部研究報告   55 ( 2 )   83 - 90   2003.3

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    サイトカイニン(CKs)の定量において、精度と感度の向上のために、安定同位体標識とコールド(非標識の天然型)標品のHPLCでの保持と MSでの質量スペクトルの特性を試験Lた。ODSとPhタイプのカラムの両方で、ゼアフチン(Z)とゼアチンリボシド(ZR)の両者とも、重水素ラベルの標品がコールドよりも早く溶出した。negative modeにおいて、ZRの m/z133/134と218の消長のパターンはZ と同様で、2H5-ZRでは分子イオンの m/z355巴断片イオンの133/134と223が見られた。 positive modeにおいて、 Z では、 20Vから60V で分子イオンの m/z220と断片の136が見られた。2H5-Z では、 Z の質量スペクトルの変化と同様に、分子イオンの m/z225と断片の137/136が見られた。 ZR では、 60V までは分子イオンのm/z352が見られ、 m/z136/137と220の消長のパターンはZ と同様であった。しかし、negative modeと同様に、 ZR の方が高いドリフト電圧が必要であった。2H5-ZRでは、分子イオンの m/z357と断片の m/z136/137と225が見られ、これらはZR および2H5-Z のビ

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  • Retention property and Mass Spectrum of Labeled Standards of Cytokinins

    KOJIMA Kiyohide, YAZAWA Sasayuki, TAKAHASHI Miyako, SHIOTA Nozomi, BABA Tadashi, IKEDA Fukio, NAKANO Masaru, HAN Dong-sheng, NIIMI Yoshiji

    Bulletin of the Faculty of Agriculture, Niigata University   55 ( 2 )   83 - 90   2003.3

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  • Regeneration of diploid and tetraploid plants from callus-derived protoplasts of Agapanthus praecox ssp orientalis (Leighton) Leighton

    M Nakano, S Tanaka, M Oota, E Ookawa, S Suzuki, H Saito

    PLANT CELL TISSUE AND ORGAN CULTURE   72 ( 1 )   63 - 69   2003.1

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  • Ploidy estimation in Hemerocallis species and cultivars by flow cytometry

    H Saito, K Mizunashi, S Tanaka, Y Adachi, M Nakano

    SCIENTIA HORTICULTURAE   97 ( 2 )   185 - 192   2003.1

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  • Isolation and characterization of gametic microprotoplasts from developing microspores of Lilium longiflorum for partial genome transfer in the Liliaceous ornamentals

    H Saito, M Nakano

    SEXUAL PLANT REPRODUCTION   15 ( 4 )   179 - 185   2002.12

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    DOI: 10.1007/s00497-002-0153-5

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  • Plant regeneration from suspension cultures of Hosta sieboldiana

    H Saito, M Nakano

    PLANT CELL TISSUE AND ORGAN CULTURE   71 ( 1 )   23 - 28   2002.10

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    DOI: 10.1023/A:1016574513545

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  • Embryogenic callus induction from leaf explants of the Liliaceous ornamental plant, Agapanthus praecox ssp orientalis (Leighton) Leighton - Histological study and response to selective agents

    S Suzuki, M Oota, M Nakano

    SCIENTIA HORTICULTURAE   95 ( 1-2 )   123 - 132   2002.8

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    DOI: 10.1016/S0304-4238(02)00033-X

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  • プロピザミド処理したヘメロカリス懸濁培養細胞からのマイクロプロトプラストの単離とその特徴

    斉藤 宏之, 中野 優

    育種学研究   4 ( 1 )   51 - 52   2002.3

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    マイクロプロトプラストを用いた非対称融合により,ユリ科花卉園芸植物において属間の染色体添加系統を作出することを目的として,微小核を形成したヘメロカリス`ステラ・デ・オロ&#039;(Hemerocallis hybrida cv. Stella d&#039;Oro)の懸濁培養細胞からマイクロプロトプラストを単離する方法を確立した.培養細胞を2mMハイドロキシウレア(HU)で24時間,その後8μMプロピザミド(PRO)で60時間処理し,さらにPRO処理を開始して20時間後に20μMサイトカラシン-B(CB)を添加することにより,微小核の形成が効率的に誘導され,そのときの微小核形成率は19.1%であった.微小核を形成した細胞を細胞壁消化酵素溶液で処理し,単離されたプロトプラストをパーコール連続密度勾配を用いて超遠心分離を行うことにより,わずかな細胞質に囲まれた小核をもつマイクロプロトプラストが単離された.それらを孔径50,20および10μmのナイロンメッシュに連続して通すことにより,直径10μm以下のマイクロプロトプラストが1ml圧縮細胞量の培養細

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  • Agrobacterium-mediated transformation in Liliaceous ornamental plants

    Sakae Suzuki, Masaru Nakano

    Japan Agricultural Research Quarterly   36 ( 3 )   119 - 127   2002

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    DOI: 10.6090/jarq.36.119

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  • Isolation and characterization of microprotoplasts from propyzamide-treated cell suspension cultures of Hemerocallis hybrida

    Hiroyuki Saito, Masaru Nakano

    Breeding Science   52 ( 1 )   51 - 56   2002

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    DOI: 10.1270/jsbbs.52.51

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  • Preparation of microprotoplasts for partial genome transfer via microprotoplast fusion in Liliaceous ornamental plants

    Hiroyuki Saito, Masaru Nakano

    Japan Agricultural Research Quarterly   36 ( 3 )   129 - 135   2002

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    DOI: 10.6090/jarq.36.129

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  • Agrobacterium-mediated production of transgenic plants of Muscari armeniacum Leichtl. ex Bak.

    S. Suzuki, M. Nakano

    Plant Cell Reports   20 ( 9 )   835 - 841   2002

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    DOI: 10.1007/s00299-001-0405-0

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  • ヘメロカリス懸濁培養細胞における細胞分裂の同調化および微小核形成 : ハイドロキシウレアおよび様々な紡錘糸形成阻害剤の影響

    斉藤 宏之, 中野 優

    育種学研究   3 ( 4 )   2001.12

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  • Partial synchronization of cell division and micronucleation in suspension-cultured cells of Hemerocallis hybrida: The effects of hydroxyurea and various spindle toxins

    F Saito, M Nakano

    BREEDING SCIENCE   51 ( 4 )   285 - 291   2001.12

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  • Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum Leichtl. ex Bak.

    S. Suzuki, M. Nakano

    In Vitro Cellular and Developmental Biology - Plant   37 ( 3 )   382 - 387   2001

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    DOI: 10.1007/s11627-001-0067-1

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  • Flow cytometric analysis of partially synchronized suspension cultures of Hemerocallis hybrida

    Hiroyuki Saito, Masaru Nakano

    Plant Biotechnology   18 ( 3 )   229 - 231   2001

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    DOI: 10.5511/plantbiotechnology.18.229

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  • Production of transgenic plants of the Liliaceous ornamental plant Agapanthus praecox ssp. orientalis (Leighton) Leighton via Agrobacterium-mediated transformation of embryogenic calli

    Sakae Suzuki, Kanyaratt Supaibulwatana, Masahiro Mii, Masaru Nakano

    Plant Science   161 ( 1 )   89 - 97   2001

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    DOI: 10.1016/S0168-9452(01)00393-4

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  • Establishment and Characterization of Cell Suspension Cultures ofSuspension Cultures of Hemerocallis hybrida

    SAITO Hiroyuki, NAKANO Masaru

    Bulletin of the Faculty of Agriculture, Niigata University   53 ( 1 )   1 - 8   2000.12

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  • Decrease in the regeneration potential of long-term cell suspension cultures of Lilium formosanum Wallace and its restoration by the auxin transport inhibitor, 2,3,5-triiodobenzoic acid

    M Nakano, T Sakakibara, S Suzuki, H Saito

    PLANT SCIENCE   158 ( 1-2 )   129 - 137   2000.9

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  • Interspecific hybrids between Lilium nobilissimum and L-regale produced via ovules-with-placental-tissue culture

    Y Obata, Y Niimi, M Nakano, K Okazaki, Miyajima, I

    SCIENTIA HORTICULTURAE   84 ( 1-2 )   191 - 204   2000.4

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  • Production of Commercial Bulbs of Lilium rubellum Baker: Changes in Carbohydrates in Bulblets and Sugara of Liquid Medium during their Culture

    NIIMI Yoshiji, MISAKI Yutaka, NAKANO Masaru

    Engei Gakkai zasshi   69 ( 2 )   161 - 165   2000.3

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    1. Bulblets of Lilium rubellum were cultured for 16 weeks in liquid MS medium containing 100, 150 and 250 mmol&amp;middot;liter&lt;SUP&gt;-1&lt;/SUP&gt; (mM) sucrose (referred to as 100, 150 and 250mM sucrose medium, respectively). The 250mM sucrose medium enhanced bulblet growth the most. The greatest gain in fresh weight occurred between 4 and 8 weeks after culture. The ratio of dry to fresh weights (DW/FW) of bulblets cultured in the 250mM sucrose medium was always highest. 2. Three sugars (sucrose, glucose, and fructose) were detected in the autoclaved MS medium. When bulblets were cultured in the liquid sucrose medium, the concentration of glucose and fructose in the medium increased until week 4 as the sucrose concentration decreased. Sugars in the 150mM sucrose medium were nearly depleted by week 8, whereas those in the 250mM sucrose medium persisted for 12 weeks. The growth rate of the bulblets declined as sugar concentration in the medium decreased. 3. The sucrose content in bulblets, cultured in each medium, increased until week 4, then decreased. However, in bulblets, cultured in the 250mM sucrose medium, the sugar content increased again after 12 weeks of culture, while starch content

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  • Production of commercial bulbs of Lilium rubellum baker: Changes in carbohydrates in bulblets and sugars of liquid medium during their culture

    Y Niimi, Y Misaki, M Nakano

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   69 ( 2 )   161 - 165   2000.3

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  • Histological examination of callogenesis and adventitious embryogenesis in immature ovary culture of grapevine (Vitis vinifera L.) Reviewed

    M Nakano, Y Watanabe, Y Hoshino

    JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY   75 ( 2 )   154 - 160   2000.3

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  • Formation of Calli from Isolated Microspore Cultures of Asiatic Hybrid Lily 'Connecticut King'

    HAN Dong-Sheng, NIIMI Yoshiji, NAKANO Masaru

    Engei Gakkai zasshi   69 ( 1 )   52 - 56   2000.1

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    Calli from microspores were induced for the first time in Lilium species. The uninucleate microspores of the Asiatic hybrid lily &#039;Connecticut King&#039; were isolated and cultured in liquid medium, containing half strength of MS macronutrients, full strength of micronutrients, Fe-EDTA and vitamins of MS, 100 mg&amp;middot;liter&lt;SUP&gt;-1&lt;/SUP&gt; cosamino acids, 500 mg&amp;middot;liter&lt;SUP&gt;-1&lt;/SUP&gt; glutamine, 1 mg&amp;middot;liter&lt;SUP&gt;-1&lt;/SUP&gt; picloram and 0.25 M sucrose or maltose. Microspore viability and development of cultured microspores were influenced by the carbohydrate sources in culture medium. A relatively high viability rate of microspores was observed in the maltose medium, compared with that of sucrose. Cell divisions of microspores and callus formation were found in the maltose medium, whereas in the sucrose medium, a large number of swollen microspores containing many starch grains remained undivided. Hence, maltose is the preferred carbohydrate source for microspore culture of &#039;Connecticut King&#039;.

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  • Formation of calli from isolated microspore cultures of Asiatic hybrid lily 'Connecticut King'

    DS Han, Y Niimi, M Nakano

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   69 ( 1 )   52 - 56   2000.1

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  • Histological examination of callogenesis and adventitious embryogenesis in immature ovary culture of grapevine (Vitis vinifera L.)

    M. Nakano, Y. Watanabe, Y. Hoshino

    Journal of Horticultural Science and Biotechnology   75 ( 2 )   154 - 160   2000

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    DOI: 10.1080/14620316.2000.11511215

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  • Long term maintenance of an anther-derived haploid callus line of the Asiatic hybrid lily 'Connecticut King'

    DS Han, Y Niimi, M Nakano

    PLANT CELL TISSUE AND ORGAN CULTURE   61 ( 3 )   215 - 219   2000

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    DOI: 10.1023/A:1006425307530

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  • Production of Doubled Haploid Plants Through Colchicine Treatment of Anther-derived Haploid Calli in the Asiatic Hybrid Lily 'Connecticut king'

    HAN Dong-Sheng, NIIMI Yoshiji, NAKANO Masaru

    Engei Gakkai zasshi   68 ( 5 )   979 - 983   1999.9

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    An effective method for producing doubled haploid plants of the Asiatic hybrid lily &#039;Connecticut King&#039; was established with an in vitro colchicine treatment of haploid calli. With an increase in the concentration and duration of colchicine treatment for the haploid calli, both the survival rate and the shoot regeneration from the calli decreased, but the frequency of diploid cells in the callus increased. Treatments of 0.25 and 0.5 mM colchicine for 48 or 72 hrs induced doubled haploid cells and shoot differentiation from calli. Haploid and diploid plantlets were regenerated from colchicine-treated calli, and only haploid plantlets were formed in colchicine-free treatments. This result suggests that these diploid plantlets originated from doubled haploid cells. Double haploids developed bulblets with more scaly leaves with longer stomatal guard cells than did the haploid plantlets.

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  • Production of doubled haploid plants through colchicine treatment of anther-derived haploid calli in the Asiatic hybrid lily 'Connecticut King'

    DS Han, Y Niimi, M Nakano

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   68 ( 5 )   979 - 983   1999.9

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  • Effect of Plant Growth Regulators on Pollen Tube Behavior and SeedFormation in Self-pollinated Flowers of Lilium longiflorum Thunb.` Georgia 'and ` Hinomoto '

    NIIMI Yoshiji, LI Tong-Hua, NAKANO Masaru

    Bulletin of the Faculty of Agriculture, Niigata University   52 ( 1 )   1 - 10   1999.8

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  • Production of Transgenic Plantlets of Carnation via Agrobacterium-mediated Transformation of Petal Explants

    Nakano Masaru, Koike Yosuke, Watanabe Yusuke

    Bulletin of the Faculty of Agriculture, Niigata University   51 ( 2 )   105 - 114   1999.3

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  • Adventitious shoot regeneration and micropropagation of hybrid tuberous begonia (Begonia x tuberhybrida Voss)

    M Nakano, Y Niimi, D Kobayashi, A Watanabe

    SCIENTIA HORTICULTURAE   79 ( 3-4 )   245 - 251   1999.2

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  • Effect of Temperature and Illuminating Conditions on Regeneration and Development of Bulblets in Scale Culture of Seven Lilium Spp.

    NIIMI Yoshiji, NAKANO Masaru, ISOGAI Namiko

    Engei Gakkai zasshi   68 ( 1 )   28 - 34   1999.1

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    Excised scales of lily bulbs were kept at 15&amp;deg;C and 25&amp;deg;C, and under continuous light or dark to study the regeneration and development of bulblets. The seven species were examined : Lilium rubellum Baker, L. speciosum Thunb. &#039;Uchida&#039;, L. nobilissimum Makino, L. formosanum Wallace, L. longiflorum Thunb. &#039;Hinomoto&#039;, L. maculatum Thunb., and the Asiatic hybrid L. X &#039;Benisugata&#039;. 1. Generally, more bulblets were regenerated at 25&amp;deg;C than at 15&amp;deg;C in all Lilium spp : bulblets of L. rubellum and L. X &#039;Benisugata&#039; were formed equally well at 15&amp;deg;C and 25&amp;deg;C. Regardless of temperatures, more bulblets of L. formosanum, L. longiflorum &#039;Hinomoto&#039;, and L. X &#039;Benisugata&#039; regenerated in the light more than they did in the dark. 2. Regenerated bulblets grew better under light at 25&amp;deg;C than at 15&amp;deg;C and the light stimulated the formation of scaly leaves from bulblets of all species and cultivars, except in L. nobilissimum. In L. nobilissimum, bulblets failed to form scaly leaves under any cultural conditions. Growth of bulblets of L. formosanum, L. longiflorum &#039;Hinomoto&#039;, and L. maculatum was promoted in darkness, whereas the bulblets of L. rubellum and L. nobilissimum gre

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  • Effects of temperature and illuminating conditions on regeneration and development of bulblets in scale culture of seven Lilium spp.

    Y Niimi, M Nakano, N Isogai

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   68 ( 1 )   28 - 34   1999.1

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  • Production of transgenic grapevine ( Vitis vinifera L. cv. Koshusanjaku) plants by Co- cultivation of embryogenic calli with agrobacterium tumefaciens and selecting secondary embryos

    Yogic Hoshino, Yan-Ming Zhu, Masaru Nakano, Eikichi Takahashi, Masahiro Mii

    Plant Biotechnology   15 ( 1 )   29 - 33   1998

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    DOI: 10.5511/plantbiotechnology.15.29

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  • Effects of several antibiotics and bialaphos on the growth and organ formation of lilium formosanum calli and transient expression of the gusa gene after co cultivation with agrobacterium tumefaciens

    Sakae Suzuki, Yoshiji Niimi, Toshiaki Sakakibara, Keizo Hosokawa, Saburo Yamamura, Masaru Nakano

    Plant Biotechnology   15 ( 4 )   213 - 216   1998

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    DOI: 10.5511/plantbiotechnology.15.213

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  • Production of commercial Lilium rubellum baker bulbs: Effects of volume and renewal of liquid medium on in vitro growth, bulb rot infection during cold treatment, and post-in-vitro growth of bulblets

    Yoshiji Niimi, Masaru Nakano, Shigemi Saito

    Journal of the Japanese Society for Horticultural Science   66   113 - 119   1997.12

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    Bulblets of Lilium rubellum Baker were cultured for 16 weeks in a 20-, 30- or 40-ml liquid medium renewed 0, 1, or 3 times. The bulblets were assessed for growth and rot infection. 1. Bulblets with fresh weight of more than 600 mg were obtained by using a 20- or 30-ml medium with 3 renewals, or 40 ml with one renewal. 2. Bulblets frequently turned brown when they were cultured in a 30- or 40-ml medium with 3 renewals: they frequently rotted both during a cold treatment and after transplantation. 3. Bulblets cultured in a 20-ml medium did not rot after a cold treatment irrespective of the number of medium renewals; they grew well after transplantation to the greenhouse. 4. Bulblets cultured in a 20-ml medium with 3 renewals most frequently developed into plantlets with elongated axes 15 weeks after transplantation. These results indicate that the 20-ml medium with 3 renewals appears to be most suitable for bulblet culture of L. rubellum in a liquid medium.

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  • Establishment of embryogenic cultures in several cultivars of Vitis vinifera and V. x labruscana

    M Nakano, T Sakakibara, Y Watanabe, T Mii

    VITIS   36 ( 3 )   141 - 145   1997.9

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  • Production of Commercial Lilium rubellum Baker Bulls: Effects of Volume and Renewal of Loquid Medium on In Vitro Growth, Bulb Rot Infection during Cold Treatment, and Post-in-vitro Growth of Bulblets

    NIIMI Yoshiji, NAKANO Masaru, SAITO Shigemi

    Engei Gakkai zasshi   66 ( 1 )   113 - 119   1997.6

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    Bulblets of &lt;I&gt;Lilium rubellum&lt;/I&gt; Baker were cultured for 16 weeks in a 20-, 30- or 40-ml liquid medium renewed 0, 1, or 3 times. The bulblets were assessed for growth and rot infection.&lt;BR&gt;1. Bulblets with fresh weight of more than 600 mg were obtained by using a 20- or 30-ml medium with 3 renewals, or 40 ml with one renewal.&lt;BR&gt;2. Bulblets frequently turned brown when they were cultured in a 30- or 40-ml medium with 3 renewals; they frequently rotted both during a cold treatment and after transplantation.&lt;BR&gt;3. Bulblets cultured in a 20-ml medium did not rot after a cold treatment irrespective of the number of medium renewals; they grew well after transplantation to the greenhouse.&lt;BR&gt;4. Bulblets cultured in a 20-ml medium with 3 renewals most frequently developed into plantlets with elongated axes 15 weeks after transplantation.&lt;BR&gt;These results indicate that the 20-ml medium with 3 renewals appears to be most suitable for bulblet culture of &lt;I&gt;L. rubellum&lt;/I&gt; in a liquid medium.

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  • Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L) through somatic embryogenesis by using embryogenic callus culture and activated charcoal

    YM Zhu, Y Hoshino, M Nakano, E Takahashi, M Mii

    PLANT SCIENCE   123 ( 1-2 )   151 - 157   1997.3

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  • Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily 'Connecticut King'

    DS Han, Y Niimi, M Nakano

    PLANT CELL TISSUE AND ORGAN CULTURE   47 ( 2 )   153 - 158   1997

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    DOI: 10.1007/BF02318951

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  • Pollen Tube Growth and Seed Set in Stigmatic and Cut-style Pollinated Lilium longiflorum 'Georgia' Flowers as Influenced by Pre-pollination

    LI Tong hua, NIIMI Yoshiji, NAKANO Masaru

    Engei Gakkai zasshi   65 ( 1 )   135 - 144   1996.6

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    The present study was conducted to examine whether pre-pollination is effective in improving growth of pollen tubes and seed-sets of &lt;I&gt;Lilium longiflorum&lt;/I&gt; &#039;Georgia&#039; by comparing cut-style pollination (CSP) and stigmatic pollination (SP).&lt;BR&gt;1. Pollen tubes reached the basal end of the style by 48 hr in self-pollination of &#039;Georgia&#039; and cross-pollination of &#039;Georgia&#039; &amp;times; &#039;Hinomoto&#039; by CSP. Using the pre-pollination technique in CSP enhanced the growth of pollen tubes both in self-and cross-pollination.&lt;BR&gt;2. Generative cell division of pollen tubes in the style was observed 24 hr after pollination. In cross-pollination by SP, 78% of the pollen tubes had 2 sperm nuclei and 1 vegetative nucleus (&#039;2 S + V&#039;), while only 15% of these appeared in the self-combination. Using CSP, pollen tubes with &#039;2 S+ V&#039; were 32%in cross-and 9%in self-pollination. The percentages increased to 54% and 35%, respectively, when pollen of &#039;Hinomoto&#039; was used in the pre-pollination treatment.&lt;BR&gt;3. In SP, numerous pollen tubes which penetrated the style grew in a bunch between two rows of ovules in an ovary ; they reached the ovules near the mid-region of the loculi. In CSP, pollen tubes elongated poor

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  • Production of Interspecific Hybrids between Lilium regale and L. rubellum via Ovule Culture

    NIIMI Yoshiji, NAKANO Masaru, MAKI Ken-ichiro

    Engei Gakkai zasshi   64 ( 4 )   919 - 925   1996.3

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    &lt;I&gt;Lilium regale&lt;/I&gt; and &lt;I&gt;L. rubellum&lt;/I&gt; were crossed reciprocally to introduce economically and horticulturally desirable traits of &lt;I&gt;L. regale&lt;/I&gt;, such as vigorous growth and disease resistance, into &lt;I&gt;L. rubellum&lt;/I&gt;.&lt;BR&gt;1. In &lt;I&gt;L. regale&lt;/I&gt; &amp;times;&lt;I&gt;L. rubellum&lt;/I&gt;, seeds containing an embryo were obtained with a low frequency (3.3 %) by stigmatic pollination at anthesis, but they did not germinate in soil or in vitro. Seedlings were successfully obtained with frequencies of 5.3 to 6.7 % by applying ovule culture 30 to 60 days after pollination.&lt;BR&gt;2. In &lt;I&gt;L. rubellum&lt;/I&gt; &amp;times;&lt;I&gt;L. regale&lt;/I&gt;, the growth of pollen tubes was inhibited in the style after stigmatic pollination at anthesis; hence, no ovule with an embryo was obtained. Growth of pollen tubes was promoted by stigmatic pollination when the pollination was done 2 to 5 days after anthesis. Embryos definitely formed if stigmatic pollination was carried out 5 days after anthesis, but they could not be rescued even when ovule culture was utilized. Cut-style pollination had no effect on production of ovules with an embryo.&lt;BR&gt;3. Hybridity of seedlings resulted from &lt;I&gt;L. regale&lt;/I&gt; &amp;times; &lt;I&gt;L. rubellum&lt;/I&gt; wa

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  • Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana

    Keizo Hosokawa, Masaru Nakano, Yayoi Oikawa, Saburo Yamamura

    Plant Cell Reports   15 ( 8 )   578 - 581   1996

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    DOI: 10.1007/BF00232456

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  • Intergeneric somatic hybrid plantlets between Dianthus barbatus and Gypsophila paniculata obtained by electrofusion

    M. Nakano, Y. Hoshino, M. Mii

    Theoretical and Applied Genetics   92 ( 2 )   170 - 172   1996

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    DOI: 10.1007/BF00223372

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  • Comparison of Seedling Production among Several Embryo-rescue Techniques in Lilium formosanum Wallace

    NIIMI Yoshiji, NAKANO Masaru, GOTO Masashi

    Plant tissue culture letters   12 ( 3 )   317 - 319   1995.12

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    タカサゴユリ (&lt;i&gt;Lilium formosanum&lt;/i&gt;) において, 幼胚からの実生獲得方法を確立するために, 4通りの胚救出方法 (胚培養, 胚珠培養, 胎座付き胚珠培養, 子房輪切り培養) を比較したところ, 胚珠培養, 胎座付き胚珠培養および子房輪切り培養を行った場合には, 自家受粉後10日目の幼胚からも実生を獲得することができた. 特に, 胎座付き胚珠培養は比較的簡便に行え, また外植片の汚染も少ないといった点から, 最も実用的であると考えられた.

    DOI: 10.5511/plantbiotechnology1984.12.317

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  • PLANT-REGENERATION FROM PROTOPLASTS OF GENTIANA BY EMBEDDING PROTOPLASTS IN GELLAN GUM

    M NAKANO, K HOSOKAWA, T OOMIYA, S YAMAMURA

    PLANT CELL TISSUE AND ORGAN CULTURE   41 ( 3 )   221 - 227   1995.6

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    DOI: 10.1007/BF00045085

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  • Plant Regeneration from Protoplasts in Dianthus: Comparison of Cultural Behavior of Different Donor Tissues

    NAKANO Masaru, MII Masahiro

    Plant tissue culture letters   12 ( 1 )   62 - 67   1995.4

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    Protoplast isolation, culture and shoot regeneration from protoplast-derived calli were compared among different organs of &lt;i&gt;Dianthus&lt;/i&gt; species under the same conditions. Leaves, petals and seedling hypocotyls of &lt;i&gt;D. caryophyllus&lt;/i&gt; cv. Chabaud (a seed-propagated cultivar) and &lt;i&gt;D. barbatus&lt;/i&gt; were used as protoplast sources. Protoplasts isolated from petals of these two species showed only low yield and low division frequency. On the other hand, division frequency of hypocotyl protoplasts was higher than that of leaf protoplasts, although, in &lt;i&gt;D. caryophyllus&lt;/i&gt;, protoplast yield in hypocotyls was slightly lower than in leaves. In both species, relatively high frequency of shoot regeneration was obtained in hypocotyl- and petal-derived protoplasts, while only low or no shoot regeneration occurred in leaf-derived protoplasts.

    DOI: 10.5511/plantbiotechnology1984.12.62

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  • PLANT-REGENERATION FROM CELL SUSPENSION-DERIVED PROTOPLASTS OF SAINTPAULIA-IONANTHA WENDL

    Y HOSHINO, M NAKANO, M MII

    PLANT CELL REPORTS   14 ( 6 )   341 - 344   1995.3

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    DOI: 10.1007/BF00238593

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  • Regeneration of transgenic plants of grapevine (Vitis vinifera L.) via Agrobacterium rhizogenes-mediated transformation of embyronic calli

    M. Nakano, Y. Hoshino, M. Mii

    Journal of Experimental Botany   45   649 - 656   1994.1

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  • ADVENTITIOUS SHOOT REGENERATION FROM CULTURED PETAL EXPLANTS OF CARNATION

    M NAKANO, Y HOSHINO, M MII

    PLANT CELL TISSUE AND ORGAN CULTURE   36 ( 1 )   15 - 19   1994.1

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    DOI: 10.1007/BF00048310

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  • SOMATIC HYBRIDIZATION BETWEEN DIANTHUS-CHINENSIS AND D-BARBATUS THROUGH PROTOPLAST FUSION

    M NAKANO, M MII

    THEORETICAL AND APPLIED GENETICS   86 ( 1 )   1 - 5   1993.3

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    DOI: 10.1007/BF00223802

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  • CALLUS AND ROOT-FORMATION FROM AN INTERGENERIC SOMATIC HYBRID BETWEEN DIANTHUS-CARYOPHYLLUS AND GYPSOPHILA-PANICULATA

    M NAKANO, M MII

    SCIENTIA HORTICULTURAE   53 ( 1-2 )   13 - 19   1993.1

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    DOI: 10.1016/0304-4238(93)90133-B

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  • Antibiotics Stimulate Somatic Embryogenesis without Plant Growth in Several Dianthus Cultivars

    Masaru Nakano, Masahiro Mii

    Journal of Plant Physiology   141 ( 6 )   721 - 725   1993

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    DOI: 10.1016/S0176-1617(11)81581-6

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  • INTERSPECIFIC SOMATIC HYBRIDIZATION IN DIANTHUS - SELECTION OF HYBRIDS BY THE USE OF IODOACETAMIDE INACTIVATION AND REGENERATION ABILITY

    M NAKANO, M MII

    PLANT SCIENCE   88 ( 2 )   203 - 208   1993

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    DOI: 10.1016/0168-9452(93)90092-E

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  • Application of Plant Biotechnology for Breeding of Flower Crops in the Genus Dianthus.

    NAKANO Masaru, MII Masahiro

    Plant tissue culture letters   10 ( 2 )   115 - 122   1993

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    DOI: 10.5511/plantbiotechnology1984.10.115

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  • PROTOPLAST CULTURE AND PLANT-REGENERATION OF SEVERAL SPECIES IN THE GENUS DIANTHUS

    M NAKANO, M MII

    PLANT CELL REPORTS   11 ( 5-6 )   225 - 228   1992.6

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    DOI: 10.1007/BF00235070

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  • SHOOT REGENERATION FROM SPINACH HYPOCOTYL SEGMENTS BY SHORT-TERM TREATMENT WITH 5,6-DICHLORO-INDOLE-3-ACETIC ACID

    M MII, M NAKANO, K OKUDA, M IIZUKA

    PLANT CELL REPORTS   11 ( 2 )   58 - 61   1992.3

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    DOI: 10.1007/BF00235253

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  • Characterization of transgenic Lilium 'Acapulco' plants containing MBW-complex-related genes of Arabidopsis thaliana

    72   1 - 6   2020.2

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    Flower and foliage colors are very important traits in ornamental plants. In most cases, anthocyanins are synthesized and accumulated in tepal or leaf cells, resulting in red, purple or violet flowers or leaves. MYB-bHLH-WDR (MBW)-complex, a ternary transcriptional complex, is known to induce anthocyanin accumulation through transcriptional upregulation of multiple genes for the flavonoid biosynthetic pathway. In the present study, we characterized transgenic Lilium 'Acapulco'plants containing the MBW-complex genes of Arabidopsis thaliana in various combination in order to modify its flower and leaf colors. Some transgenic plantlets had red-purple to deep red-purple vegetative organs (bulb scales, scaly leaves and/or roots). Transgenic plants with colored vegetative organs generally showed poor growth compared with non-transgenic plants, which may be due to an excessive accumulation of anthocyanins. Only four transgenic plants have so far reached the flowering stage, and all of them had green leaves as non-transgenic plants. Among four transgenic plants, two containing only the bHLH gene had deeper red tepals compared with non-transgenic plants. Spectrophotometer analysis showed that the total anthocyanin contents in deeper red tepals was significantly higher than non-transgenic plants. Our results indicate that ectopic expression of the heterogeneous MBW-complex genes in Lilium 'Acapulco' may activate the flavonoid biosynthetic pathway leading to anthocyanin synthesis and accumulation in vegetative and floral organs.

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    Other Link: http://hdl.handle.net/10191/00051505

  • Flower and leaf color alteration and effect on morphology in Tricyrtis sp. by introduction of carotenoid biosynthesis related gene

    市橋彰歌, 中野優, 大谷真広, 三沢典彦, 鈴木栄

    園芸学研究 別冊   19 ( 1 )   2020

  • ホトトギス(Tricyrtis sp.)由来BクラスMADS-box遺伝子のCRES-Tコンストラクトが導入されたユリ(Lilium sp.)形質転換体の作出

    青柳海輝, 大谷真広, 佐藤翔一, 小林仁, 奥原宏明, 野水利和, 近藤正剛, 菅野明, 中野優

    園芸学研究 別冊   15 ( 1 )   2016

  • ユリ科ホトトギス属植物(Tricyrtis sp.)におけるCRES-T法によるBクラスMADS-box遺伝子の機能抑制

    大谷真広, 佐藤翔一, 菅野明, 星野洋一郎, 光田展隆, 中野優

    園芸学研究 別冊   14 ( 1 )   2015

  • Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 7. Characterization of intergeneric hybrid plants of Littonia modesta × Sandersonia aurantiaca 'Phoenix'

    AMANO J, KUWAYAMA S, NAKAZAWA D, OKUNO H, GODO T, NAKANO M

    園芸学研究. 別冊, 園芸学会大会研究発表要旨   6 ( 2 )   589 - 589   2007.9

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  • Production of interspecific triploid hybrids in Tricyrtis via ovule culture

    OKA E., AMANO J., NAKANO M.

    6 ( 2 )   590 - 590   2007.9

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  • Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 6. Characterization of intergeneric hybrid plants of Sandersonia aurantiaca × Gloriosa superba 'Lutea'

    AMANO J, KUWAYAMA S, SUGAWARA S, MIZUTA Y, GODO T, NAKANO M

    園芸学研究. 別冊, 園芸学会大会研究発表要旨   6 ( 1 )   472 - 472   2007.3

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  • Production and characterization of interspecific hybrids of Lychnis 'Karafutoenbisenno' × L. sieboldii

    KUWAYAMA S, ASANO M, GODO T, NAKATA M, NAKANO M

    園芸学研究. 別冊, 園芸学会大会研究発表要旨   6 ( 1 )   473 - 473   2007.3

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  • Interspecific hybrids between triploid Senno (Lychnis senno Siebold et Zucc) and related species

    MIYAZAKI J, KUWAYAMA S, NAKANO M, OGITA S, KATO Y, NAKATA S, GODO T

    園芸学研究. 別冊, 園芸学会大会研究発表要旨   6 ( 1 )   210 - 210   2007.3

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  • Isolation and characterization of class B genes in Alstroemeria

    M Hirai, T Kamimura, M Nakada, IJ Song, T Fukuda, Y Hoshino, M Nakano, A Kanno

    PLANT AND CELL PHYSIOLOGY   47   S179 - S179   2006

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  • Production of transgenic plants via Agrobacterium-mediated transformation in Liliaceous ornamentals. Floriculture, Ornamental and Plant Biotechnology: advances and topical issues,Jaime A. (ed)

    Masaru NAKANO, Shiro MORI, Sakae SUZUKI, Yosuke HOSHI, Hitoshi KOBAYASHI

    Global Science Books TM Ltd., London,   volume II   172 - 183   2006

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  • 単子葉花き園芸植物における形質転換技術を用いた花形の改変 平成16‐18年度

    中野優

    単子葉花き園芸植物における形質転換技術を用いた花形の改変 平成16-18年度 No.16380024   146P   2006

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  • Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 4. Characterization of plants derived from Littonia modesta × Sandersonia aurantiaca

    KUWAYAMA S, AMANO J, SUGAWARA S, NAKAMURA T, MIZUTA Y, OOMIYA T, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   74 ( 2 )   643 - 643   2005.10

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  • Fertility restoration and chromosome doubling induced by γ-ray irradiation in Lippia canescens

    HOSHI Y, KONDO M, OGATA K, KUWAYAMA S, MORI S, NAKANO M, KOBAYASHI H

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   74 ( 2 )   292 - 292   2005.10

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  • Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak.

    Nakano Masaru, Tanaka Shigefumi, Kagami Shiho, Saito Hiroyuki

    Plant biotechnology   22 ( 3 )   249 - 251   2005.9

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  • Morphological characterization and analysis of uidA gene expression in transgenic plants of Tricyrtis hirta at the flowering stage

    OKA E, MORI S, ADACHI Y, HOSHI Y, KONDO M, KOBAYASHI H, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   74 ( 1 )   216 - 216   2005.3

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  • Isolation and characterization of B class MADS-box genes in Tricyrtis affinis

    NAKAMURA T., FUKUDA T., OCHIAI T., NAKANO M., KANNO A., KAMEYA T.

    73 ( 2 )   574 - 574   2004.9

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  • Effects of antibiotics and bialaphos on the growth and development of embryogenic callus cultures of Muscari armeniacum

    S. Suzuki, M. Nakano

    Biologia Plantarum   47 ( 3 )   425 - 427   2004

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  • Morphological characterization and analysis of the uidA gene expression in transgenic plants of Agapanthus praecox ssp. orientalis

    MORI S, SUZUKI S, HOSHI Y, KONDO M, KOBAYASHI H, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 2 )   283 - 283   2003.9

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  • Functional analyses of the PISTILLATA gene of agapanthus (ApPI) in transgenic lettuce plants

    MAKINO M., NAKAMURA T., NAKANO M.

    72 ( 2 )   561 - 561   2003.9

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  • Increasing the efficiency of Agrobacterium-mediated transformation by a simple treatment with sandpaper

    HOSHI Y, KONDO M, MORI S, ADACHI Y, NAKANO M, MATSUMOTO I, KOBAYASHI H

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   165 - 165   2003.4

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  • Adventitious embryogenesis and plant regeneration of several species in the genus Tricyrtis

    TANAKA S, MIZUNASHI K, NAKATA M, GODO T, SAITO H, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   380 - 380   2003.4

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  • Transformation of Lilium by using Agrobacterium

    HOSHI Y, KONDO M, MORI S, ADACHI Y, NAKANO M, MATSUMOTO I, KOBAYASHI H

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   372 - 372   2003.4

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  • Isolation and expression analysis of the dihydroflavonol 4-reductase (DFR) gene in Agapanthus spp.

    MORI S, TAKEDA Y, YAMAMOTO K, HOSHI Y, KONDO M, KOBAYASHI H, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   362 - 362   2003.4

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  • Plant regeneration from callus cultures and Agrobacterium-mediated transient expression of the gusA gene in several Lilium species

    ADACHI Y, MORI S, KOBAYASHI H, HOSHI Y, KONDO M, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   167 - 167   2003.4

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  • Isolation and characterization of flavonoid 3'5'-hydroxylase (F3'5'H) gene from Vinca major L.

    MORI S, KOBAYASHI H, HOSHI Y, KONDO M, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   71 ( 2 )   494 - 494   2002.10

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  • A protoplast-to-plant system in Muscari armeniacum Leichtl. ex Bak.

    TANAKA S., SAITO H., KAGAMI S., NAKANO M.

    71 ( 2 )   234 - 234   2002.10

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  • Regeneration of diploid and tetraploid plants from callus-derived protoplasts of Agapanthus praecox ssp. orientalis (Leighton) Leighton

    N. Masaru, TANAKA S., OOTA M., OOKAWA E., SUZUKI S., SAITO H.

    71 ( 2 )   521 - 521   2002.10

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  • Analyses of anthocyanidin and anthocyanin in petals of Muscari spp.

    MORI S, ASANO S, KOBAYASHI H, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   71 ( 1 )   180 - 180   2002.4

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  • Ploidy estimation in Hemerocallis species by flow cytometer

    SAITO H., MIZUNASHI K., TANAKA S., ADACHI Y., NAKANO M.

    71 ( 1 )   150 - 150   2002.4

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  • Isolation of the MADS-box genes which specifically expressed in floral organs of Agapanthus praecox ssp.

    NAKAMURA T., KANNO A., NAKANO M.

    4 ( 1 )   18 - 18   2002.3

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  • Production of transgenic plants of Muscari armeniacum via Agrobacterium-mediated transformation

    SUZUKI S., NAKANO M.

    70 ( 2 )   433 - 433   2001.9

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  • Production of intergeneric hybrid plants between Sandersonia aurantiaca and Gloriosa rothchildiana via ovule culture

    NAKAMURA T., NAKANO M.

    70 ( 2 )   420 - 420   2001.9

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  • Studies on the partial genome transfer via microprotoplast fusion in the Liliaceous ornamental plants : V. Isolation and characterization of microprotoplasts from developing microspores in Lilium longiflorum

    SAITO H., NAKANO M.

    70 ( 2 )   432 - 432   2001.9

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  • Studies on the partial genome transfer via microprotoplast fusion in the Liliaceous ornament al plants. IV. Effect of several spindle toxins on the micronucleation of developing microspores in Lilium spp. Hemerocallis hybrida.

    SAITO H., NAKANO M.

    2 ( 2 )   120 - 120   2000.9

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  • Efficient induction of micronucleation by propyzamide treatments in suspension-cultured cells of Hemerocallis hybrida

    SAITO H., NAKANO M.

    2 ( 1 )   156 - 156   2000.4

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  • Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum

    SUZUKI S., NAKANO M.

    69 ( 1 )   333 - 333   2000.3

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  • Studies on In Vitro Haploid Production in Lilium spp : 5. Formation of Haploid Calli from Anther Cultures and Haploid Plant Regeneration in Lilium formosanum A. Wallace

    HAN D., NIIMI Y., AOKI A., NOMIZU T., NAKANO M.

    69 ( 1 )   127 - 127   2000.3

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  • Production of transgenic plants of Angelonia salicariifolia via Agrobacterium rhizogenes-mediated transformation

    KOIKE Y., HOSHINO Y., MII M., NAKANO M.

    69 ( 1 )   317 - 317   2000.3

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  • Studies on in vitro haploid production in Lilium spp : 4. Establishment and long-term maintenance of an anther-derived haploid callus line in the Asiatic hybrid 'Connecticut King'

    HAN D. S., NIIMI Y., NAKANO M.

    68 ( 2 )   372 - 372   1999.10

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  • アグロバクテリウム法によるアガパンサス形質転換体の作出

    鈴木 栄, 新美 芳二, 石森 崇晃, SUPAIBULWATANA Kanyaratt, 三位 正洋, 中野 優

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   68 ( 2 )   373 - 373   1999.10

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  • ブドウ子房からの不定胚形成の組織学的観察

    渡辺 祐輔, 中野 優

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   67 ( 2 )   208 - 208   1998.10

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  • ヘメロカリスの懸濁培養細胞を用いた細胞分裂の同調化

    斉藤 宏之, 中野 優

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   67 ( 2 )   418 - 418   1998.10

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  • 胚珠培養により得られたタモトユリとリーガルリリーの種間雑種の特性

    小畑 ユミ, 新美 芳二, 中野 優, 岡崎 桂一

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   67 ( 2 )   399 - 399   1998.10

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  • Comparison of pistil receptivity in several Lilium spp.

    KIM H. J., NIIMI Y., NAKANO M.

    66 ( 2 )   584 - 585   1997.8

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  • Regeneration of adventitious shoots from anther culture-derived calli and embryoids in several cultivars of Japanese pear (Pyrus serotina Reh d.) and apple (Malus pumila Mill.)

    KADOTA M., NIIMI Y., NAKANO M.

    66 ( 2 )   98 - 99   1997.8

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  • Studies on in vitro haploid production in Lilium spp. : 3. Effect of colchicine treatment on the ploidy level of haploid calli and regenerated plantlets in the Asiatic hybrid 'Connecticut King'

    HAN D. S., NIIMI Y., NAKANO M.

    66 ( 2 )   476 - 477   1997.8

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  • Studies on the response of calli to selective agents for obtaining transgenic plants in Lilium formosanum and transient expression of the GUS gene after co-cultivation with Agrobacterium

    SUZUKI S., NIIMI Y., SAKAKIBARA T., HOSOKAWA K., YAMAMURA S., NAKANO M.

    66 ( 2 )   488 - 489   1997.8

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  • Studies on in vitro haploid production in Lilium spp. : 2. Isolated microspore culture of the Asiatic hybrid'Connecticut King'

    HAN D. S., NIIMI Y., NAKANO M.

    66 ( 1 )   422 - 423   1997.3

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  • Somatic embryogenesis and plant regeneration from long-term cell suspension cultures of Lilium formosanum Wallace

    SAKAKIBARA T., NAKANO M., NIIMI Y.

    65 ( 2 )   622 - 623   1996.8

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  • Induction of embryogenic lines in several grapevine cultivars (Vitis spp.)

    NAKANO M., HOSHINO Y., SAKAKIBARA T., MII M.

    65 ( 2 )   262 - 263   1996.8

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  • Production of commercial bulbs in Lilium rubellum Baker : 3. Effect of ABA and fluridone on development of bulblets cultured in liquid medium

    MISAKI Y., NIIMI Y., NAKANO M.

    65 ( 2 )   612 - 613   1996.8

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  • Studies on in vitro haploid production Lilium spp. : 1. Regeneration of haploid plants from anther cultures in Asiatic hybrid 'Connecticut King'

    HAN D. S., NIIMI Y., NAKANO M.

    64 ( 2 )   480 - 481   1995.8

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  • Effects of hormone treatment, heat treatment, and preliminary pollination on seed formation in self-pollination of Lilium longiflorum cv. Georgia

    NIIMI Y., LI T.-H, NAKANO M.

    64 ( 1 )   398 - 399   1995.3

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  • Somatic hybrid callus formation and root regeneration between carnation and gypsophila by protoplast fusion

    NAKANO M., MII M.

    Japanese Journal of Breeding   38 ( 2 )   170 - 171   1988

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Research Projects

  • Elucidation of the mechanism of controlling the degree of tepal expansion at the full-bloom stage in Tricyrtis

    Grant number:23K05206

    2023.4 - 2026.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

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  • Elucidation of the molecular mechanism of spot formation in flower tepals of Tricyrtis spp.

    Grant number:19K06028

    2019.4 - 2023.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

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  • Elucidation of a molecular mechanism of inflorescence development in Tricyrtis spp.

    Grant number:15K07287

    2015.4 - 2018.3

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    Nakano Masaru

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    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    The relation between inflorescence architecture and LEAFY (LFY)-like gene was examined by using two Tricyrtis spp. (T. hirta and T. formosana) showing different inflorescence architectures. No differences in the structure, function and expression pattern in young plants of the LFY-like genes were observed between these species. Further expression analysis of the LFY-like genes is necessary at the flowering stage. Transgenic plants ectopically expressing the LFY-like genes showed dwarf and early-flowering phenotypes, indicating a possibility of breeding of ornamental plants by genetic transformation with the LFY-like genes. TFL1-like genes, which have been reported to be expressed antagonistically with LFY-like genes, were then isolated from T. hirta and T. formosana. However, no difference in the estimated amino acid sequence of the TFL1-like genes was observed between these species.

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  • Development of a new cultivar production system using transgenic plants in ornamental plants

    Grant number:23580037

    2011 - 2013

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    NAKANO MASARU

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    Grant amount:\5460000 ( Direct Cost: \4200000 、 Indirect Cost:\1260000 )

    White-flowered transgenic plants resulted from RNAi suppression of the CHS gene and dwarf transgenic plants resulted from overexpression of the GA2ox gene were selected as breeding materials through characterization of various transgenic plants of Tricyrtis spp. Interspecific hybrids were produced via ovule culture when dwarf transgenic plants were used as parents. Some of these hybrids showed not only dwarf phenotypes due to the transgene but also novel flower characteristics compared with the parents, indicating the possibility of interspecific hybridization using transgenic plants in the improvement programs of ornamental plants. On the other hand, induction of somaclonal variants and chromosome doubling were also examined by using embryogenic callus cultures of transgenic plants. Detailed characterization of callus culture-derived plants are now in progress.

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  • Production of interspecific and intergeneric hybrids and analysis of genetic relationship in colchicaceous ornamentals

    Grant number:20580023

    2008 - 2010

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    NAKANO Masaru

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    Interspecific and intergeneric cross pollination was performed using several colchicaceous ornamental plants. Following ovule culture, intergeneric hybrids were successfully obtained among Gloriosa spp., Littonia modesta and Sandersonia aurantiaca. These intergeneric hybrids had novel morphological characteristics compared with their parents, indicating the validity of intergeneric hybridization in the improvement programs of colchicaceous ornamentals. Since parental chromosomes in the intergeneric hybrids could be clearly discriminated by GISH sequence homology among Gloriosa, Littonia, and Sandersonia may be relatively low.

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  • Flower form alteration in monocot ornamental plants by genetic transformation

    Grant number:16380024

    2004 - 2006

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B)

    Awarding organization:Japan Society for the Promotion of Science

    NAKANO Masaru, SHIOTSUKI Akira, HOSHINO Yoichiro

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    Grant amount:\15700000 ( Direct Cost: \15700000 )

    In the present study, the following experiments were carried out in order to alter flower form in monocot ornamentals by genetic transformation.
    1. Establishment of genetic transformation systems
    An efficient and reproducible system for Agrobacterium-mediated transformation was established in Tricyrtis hirta. In addition, long-term and stable expression of a foreign gene was confirmed in transgenic plants of Agapanthus praecox ssp. orientalis and T. hirta.
    2. Isolation and characterization of MADS-box genes involved in the ABC model
    Various MADS-box genes, mainly the class B genes, were isolated from A. praecox ssp. orientalis, Muscari armeniacum, Trillium camtschatcense, Tricyrtis affinis and Alstroemeria ligtu. Expression of the class B genes was observed in both inner and outer tepals, which supports the modified ABC model.
    3. Isolation and characterization of cycloidea (CFC)-like genes
    CYC-like genes were isolated from two Alstroemeria species. Expression analyses of these genes indicate that the floral zygomorphy in monocot plants is controlled in a similar manner to dicot plants.
    4. Genetic transformation with MADS-box genes involved in the ABC model
    The class B genes isolated from A. praecox ssp. orientalis were introduced to each of T.hirta, Nicotiana tabacum and Lactuca sativa by Agrobacterium-mediated transformation. For all the three species, some transgenic plants produced flowers with apparent morphological alterations. These results indicate a possibility of molecular breeding for flower form alteration by genetic transformation with the class B MADS-box gene(s) of heterologous plant species.

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  • A fundamental research for genetic transformation via microprotoplast fusion in Liliaceous species

    Grant number:13660024

    2001 - 2003

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (C)

    Awarding organization:Japan Society for the Promotion of Science

    NAKANO Masaru

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    Grant amount:\3400000 ( Direct Cost: \3400000 )

    Toward the application of microprotoplast fusion to genetic improvement of Liliaceous ornamental plants, 1)establishment of regenerable callus or cell suspension cultures, 2)development of protoplast culture systems, and 3)development of systems for isolating microprotoplasts, were examined.
    1)Highly regenerable callus cultures were established in Agapanthus praecox ssp. orientalis and Muscari armeniacum, and highly regenerable cell suspension cultures were established in Hosta sieboldiana.
    2)By utilizing callus cultures established, protoplast culture systems were developed in A. praecox ssp. orientalis and M. armeniacum. However, further studies are necessary to improve the efficiencies of callus formation from protoplasts and plant regeneration from protoplast-derived calluses.
    3)Two different systems for isolating microprotoplasts, one from partially synchronized cell suspension cultures of Hemerocallis hybrida(somatic microprotoplasts) and the other from developing microspores of Lilium longiflorum(gametic microprotoplasts) were developed. As compared with the somatic microprotoplast system, the gametic system seems to be more practical in Liliaceous ornamental plants.
    The systems developed in the present study may pave the way for the transfer of one or a few chromosomes via microprotoplast fusion in Liliaceous ornamental plants. Chromosome addition lines produced via microprotoplast fusion may contribute to chromosome studies as well as genetic improvement in these plants.

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  • ユリ科花卉園芸植物における形質転換技術を用いた耐病性品種作出のための基礎的研究

    Grant number:11760019

    1999 - 2000

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    中野 優

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    Grant amount:\2200000 ( Direct Cost: \2200000 )

    本実験では,ユリ科花卉園芸植物において形質転換技術により耐病性品種を作出することを最終目的として,以下の項目について検討した.
    (1)培養組織からの効率的な植物体再生系の確立
    数種・品種のユリ,アガパンサスおよびムスカリにおいて,高い増殖能力および植物体再生能力を保持したカルスまたは懸濁細胞培養系を確立した.タカサゴユリ,アガパンサス,ムスカリにおいては,組織学的観察により不定胚または不定芽経由の再生が起こっていることを明らかにした.さらに,タカサゴユリにおいては,長期間の継代・維持を経た懸濁培養細胞における再生能力の低下を確認し,また,その回復にはTIBA処理が有効であることを明らかにした.
    (2)選抜薬剤耐性濃度の調査
    数種・品種のユリ,アガパンサス,ヘメロカリスおよびムスカリにおいて,培地へのハイグロマイシンまたはビアラフォスの添加により,カルスの増殖および植物体再生が完全に抑制されることを明らかにした.
    (3)アグロバクテリウム法による形質転換系の確立
    数種・品種のユリ,アガパンサスおよびムスカリにおいてアグロバクテリウムによる形質転換を試み,そのうち現在までに,アガパンサスおよびムスカリにおいて効率的な形質転換系を確立している.
    (4)抗菌性タンパク質遺伝子ホモログのクローニング
    数種のアブラナ科植物から複数のディフェンシン遺伝子ホモログを単離し,発現ベクターへの組み込みおよびアグロバクテリウムへの導入を行った.現在,アグロバクテリウム法によるアガパンサスへの導入を試みている.

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  • 導管における硝酸イオンを介した器官間情報伝達の分子機構

    Grant number:11151210

    1999

    System name:科学研究費助成事業

    Research category:特定領域研究(A)

    Awarding organization:日本学術振興会

    末吉 邦, 中野 優, 大山 卓爾

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    Grant amount:\2000000 ( Direct Cost: \2000000 )

    「目的」硝酸イオンが導管内を移動し、葉に到達する過程で、どのように硝酸同化系遺伝子の発現制御因子としての機能を発現するのかを明らかにすることを目的とする。本年度は、葉におけるNR遺伝子発現と導管内での硝酸イオンの輸送動態の関連性を詳細に解析することと、硝酸イオンの導管輸送坦体の性質について検討を加えた。
    「結果と考察」1.植物を無硝酸培地に移し硝酸イオンの供給を止めると、葉の細胞内に硝酸イオンが十分あるにもかかわらず、NRmRNA量と導管液硝酸イオン濃度は急速に低下した。このことより、葉におけるNR遺伝子の発現に関して、細胞内の硝酸イオンはシグナルとしての機能を持たず、導管液中の硝酸イオンがシグナルとして作用していると結論づけられた。2.オオムギ根にアニオンチャネルブロッカー(DIDS)を与えると、導管液中の硝酸イオン濃度が著しく減少した。一方、培地から根への硝酸イオンの吸収はDIDSで抑えられなかった。DIDSは、根の柔細胞から導管への硝酸イオンの積みこみの段階を阻害していると考えられた。すなわち、根の柔細胞から導管への硝酸イオンの積みこみ(loading)にアニオンチャネル様タンパク質の関与が考えられた。
    「今後の展望」1.今年度は、導管液中の硝酸イオンがNR遺伝子発現の誘導を引き起こすシグナルであることを示すことができた。導管は、葉の細胞にとっては細胞外空間である。今後は、細胞外シグナルとしての硝酸イオンを検知する仕組みを明らかにする必要がある。2.今年度は、導管への硝酸イオンのloadingにアニオンチャネルが関与する可能性を示すことができた。今後、チャネルの同定が必要である。

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  • Propagation and breeding of Lilium spp. by means of tissue, organ and cell suspension culture

    Grant number:09460017

    1997 - 2000

    System name:Grants-in-Aid for Scientific Research

    Research category:Grant-in-Aid for Scientific Research (B).

    Awarding organization:Japan Society for the Promotion of Science

    NIIMI Yoshiji, OKAZAKI Keiichi, NAKANO Masaru

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    Grant amount:\11800000 ( Direct Cost: \11800000 )

    (1) With a few exceptions, more bulblets were regenerated at 25℃ than 15℃, and the regenerated bulblets grew well under light condition at 25℃.(2) All plantlets regenerated in anther culture of L.× 'Enchantment' were diploid, and ca. 40% of the plantlets were virus-free as shown in the calli-induced bulblets of L.longiflorum 'Georgia'.(3) Liquid medium and renewal of the medium 8-12 weeks after culture promoted growth of L.rubellum bulblets.(4) Treatmnent of 0.25 and 0.5 mM colchicine for 48 or 72 hrs induced double haploid cells and shoots from haploid calli of L.×'Connecticut King'. (5) A hap loid callus line from anther cultures of L.×'Connecticut King' was maintained for a long term in MS medium with picloram.(6) A viability of microspores was maintained only in a mediumn with maltose, in which cell divisions and callus formation occurred.(7) In reciprocal pollination between L.nobilissimum and L.regale, ovules excised at 30 and 40 DPA developed into seedlings, whereas those excised at 40 DAP developed into seedlings in L.nobilissimum x L.regale. In L.× 'Connecticut King' x L.× 'Enchantment', ovules excised at 3 DAP developed into seedlings, when embryo culture was used together.(8) Application of nitrous oxide (NOx) to pollen mother cells at the middle stage of the first division was effective to the production of non-reduced microspores. Treatment of 0.5 mM colchicine on in vitro cultured bulbscales for 3 or 5 days induced tetraploid plantlets.(9) Almost all cell clumps from the cell suspension cultures of L.formosanum maintained regeneration potential for more than 54 months in hormone-free medium and developed many somatic embryos.(10) Hygromycin, G418 and bialaphos were suitable agents for selecting transformed calli and/or organs of L.formosanu.(11) Some calli of L.formosanum, co-cultivated with Agrobacterium tumefaciens, showed several blue spots resulted from transient or stable expression of the gusA gene.

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  • ナシにおける植物体再生方法の確立と形質転換体の作出に関する基礎的研究

    Grant number:08760024

    1996

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    中野 優

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    Grant amount:\1100000 ( Direct Cost: \1100000 )

    本実験は,形質転換技術を用いたナシの育種を最終目的として行った.まず数品種のニホンナシおよびセイヨウナシを用いて,培養組織からの効率的な植物体再生方法を検討した.休暇枝の側芽から誘導した組織培養植物体の葉片を用いて,高頻度の不定芽誘導条件を検討したところ,尿素系のサイトカイニン(TDZ,CPPU等)存在下で比較的高頻度の不定芽形成が誘導され,さらにこの不定芽形成がGAにより促進されることが明らかとなった.また,葉片からの不定芽形成には品種間差異が観察され,ニホンナシ品種よりもセイヨウナシ品種の方が概して不定芽誘導が容易であった.次に,GUS遺伝子の一時的な発現を指標として効率的なAgrobacterium接種条件の検討を行ったところ,現在までに外植体の前処理(前培養)およびアセトシリンゴン処理によりGUS遺伝子の一時的な発現が増大することが明らかとなっている.今後は,引き続きAgrobacterium接種条件の検討を行う一方で,さらに効率的な不定芽誘導条件および形質転換細胞・組織の選抜条件の検討を行う予定である.

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  • ユリのプロトプラスト培養系の確立と細胞融合による体細胞雑種作出に関する基礎的研究

    Grant number:07760027

    1995

    System name:科学研究費助成事業

    Research category:奨励研究(A)

    Awarding organization:日本学術振興会

    中野 優

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    Grant amount:\1000000 ( Direct Cost: \1000000 )

    本実験では,ユリにおける体細胞雑種の作出を最終目的として,タカサゴユリのプロトプラストからの植物体再生に関する検討を行った.まず,子球片から懸濁培養細胞を誘導し,その増殖および分化に最適な培養条件を検討したところ,培養細胞はpicloram存在下では脱分化状態で増殖し,picloram非存在下では不定芽あるいは不定胚を経由して植物体を再生することを明らかにした.この培養細胞は,誘導後2年を経過しても高い増殖・分化能力を維持しており,また染色体数レベルでの変異はほとんど認められていない.次に,糖無添加の培地で培養細胞を1-2週間前処理することにより,活性の高いプロトプラストが高収量で得られることを明らかにした.また,プロトプラストの分裂誘導には,ナ-ス細胞を用いた保護培養の適用が効果的であることが明らかとなった.現在プロトプラスト由来カルスからの植物体再生を検討している.

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Teaching Experience

  • 応用生命・食品科学セミナーII

    2024
    Institution name:新潟大学

  • 応用農力

    2024
    Institution name:新潟大学

  • スタディ・スキルズAIa

    2023
    Institution name:新潟大学

  • 基礎農力

    2023
    Institution name:新潟大学

  • スタディ・スキルズAIb

    2023
    Institution name:新潟大学

  • 地域交流サテライト実習

    2023
    Institution name:新潟大学

  • スタディ・スキルズAIIb

    2023
    Institution name:新潟大学

  • スタディ・スキルズAIIa

    2023
    Institution name:新潟大学

  • 応用生命・食品科学概論

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIc

    2021
    Institution name:新潟大学

  • 植物細胞工学特論

    2021
    Institution name:新潟大学

  • スタディ・スキルズAIIc

    2021
    Institution name:新潟大学

  • Topics in Applied Bioresource Chemistry

    2021
    Institution name:新潟大学

  • 植物生産学演習I

    2021
    Institution name:新潟大学

  • 農学入門II

    2021
    Institution name:新潟大学

  • 応用生命科学特論

    2021
    Institution name:新潟大学

  • 農学入門I

    2021
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  • 産業スペシャリスト育成特論

    2020
    -
    2021
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  • リフレクションデザインIII

    2020
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  • 生物学実験

    2019
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  • 植物細胞工学

    2019
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  • 農学入門Ⅱ

    2019
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  • 農学入門Ⅰ

    2019
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  • 生命を知る

    2018
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  • リフレクションデザインII

    2017
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    2020
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  • 領域概説 F (農学)

    2017
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    2020
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  • ダブルホーム活動演習

    2017
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  • 生命・食料科学博士セミナーⅡ

    2013
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  • 外国語論文解説・討論Ⅱ

    2013
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  • 生命・食料科学博士特定研究Ⅱ

    2013
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  • 研究発表演習(中間発表)

    2012
    -
    2015
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  • 研究発表

    2012
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    2015
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  • 生命・食料科学セミナーAⅡ

    2012
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    2015
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  • 文献詳読Ⅰ

    2012
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    2015
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  • 生命・食料科学特定研究AⅡ

    2012
    -
    2015
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  • 文献詳読Ⅱ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学特定研究AⅠ

    2012
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    2015
    Institution name:新潟大学

  • 生命・食料科学セミナーAⅠ

    2012
    -
    2015
    Institution name:新潟大学

  • 生命・食料科学博士セミナーⅠ

    2012
    Institution name:新潟大学

  • 生物資源科学コース演習Ⅰ

    2012
    Institution name:新潟大学

  • 生命・食料科学博士特定研究Ⅰ

    2012
    Institution name:新潟大学

  • リサーチキャンプ

    2012
    Institution name:新潟大学

  • 外国語論文解説・討論Ⅰ

    2012
    Institution name:新潟大学

  • 植物生産学実験実習Ⅱ

    2010
    -
    2019
    Institution name:新潟大学

  • 植物生産学実験実習Ⅰ

    2010
    -
    2019
    Institution name:新潟大学

  • 花卉園芸学

    2009
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  • 作物及び園芸学概論

    2009
    -
    2017
    Institution name:新潟大学

  • 農作物概論

    2009
    -
    2010
    Institution name:新潟大学

  • 植物生産学演習Ⅱ

    2008
    -
    2021
    Institution name:新潟大学

  • 実地見学

    2008
    -
    2018
    Institution name:新潟大学

  • 植物生産学演習Ⅰ

    2008
    -
    2018
    Institution name:新潟大学

  • 生物資源論

    2008
    -
    2015
    Institution name:新潟大学

  • 植物生産学実験Ⅱ

    2008
    -
    2010
    Institution name:新潟大学

  • 植物生産学実験Ⅰ

    2008
    -
    2009
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  • 植物専門実習Ⅰ

    2008
    -
    2009
    Institution name:新潟大学

  • 植物生産学演習I

    2007
    -
    2021
    Institution name:新潟大学

  • 植物組織培養学特論

    2007
    -
    2019
    Institution name:新潟大学

  • 植物細胞育種学

    2007
    -
    2019
    Institution name:新潟大学

  • 植物組織培養学

    2007
    -
    2018
    Institution name:新潟大学

  • 植物生産学演習II

    2007
    Institution name:新潟大学

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