Updated on 2025/06/12

写真a

 
TANAKA Takaaki
 
Organization
Academic Assembly Institute of Science and Technology SEISAN DESIGN KOUGAKU KEIRETU Professor
Graduate School of Science and Technology Advanced Materials Science and Technology Professor
Faculty of Engineering Department of Engineering Professor
Title
Professor
Homepage
http://tctanaka.eng.niigata-u.ac.jp/top.html
External link

Degree

  • 博士（農学） ( 1996.3   京都大学 )

  • 工学修士 ( 1989.3   京都大学 )

Research Interests

  • porous materials

  • 分離材料

  • (bio)mechanical evaluation

  • bioengineering

  • membrane

  • Biofunction Bioprocess

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Transport phenomena and unit operations

  • Nanotechnology/Materials / Structural materials and functional materials

  • Life Science / Applied microbiology

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

  • Life Science / Applied biochemistry

Research History

  • Niigata University   Faculty of Engineering Department of Engineering   Professor

    2017.4

  • Niigata University   Graduate School of Science and Technology Advanced Materials Science and Technology   Professor

    2013.4

  • Niigata University   Graduate School of Science and Technology Advanced Materials Science and Technology   Professor

    2013.4

  • Niigata University   Faculty of Engineering Department of Material Science and Technology   Professor

    2013.4 - 2017.3

  • Niigata University   Faculty of Engineering Department of Material Science and Technology   Associate Professor

    2004.4 - 2013.3

  • Niigata University   Faculty of Engineering Department of Material Science and Technology   Associate Professor (as old post name)

    1999.1 - 2004.3

  • Niigata University   Faculty of Engineering Department of Material Science and Technology   Lecturer

    1996.5 - 1998.12

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Professional Memberships

 

Papers

  • Preparation of composite monoliths of poly(methyl methacrylate) and hydroxyapatite for protein separation Reviewed

    Takaaki Tanaka, Tomoyuki Hirai, Masaki Numada, Shotaro Ishikawa, Marino Fujisawa, Natsumi Takahashi, Ai Yamada, Akihito Ochiai

    Separation Science Plus   8 ( 6 )   e70066 - e70066   2025.6

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

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  • Composite Membranes of Polyhydroxyalkanoate and Cellulose for Depth Filtration

    Takaaki Tanaka, Kota Maruyama, Fumiya Dakeishi, Akihito Ochiai

    Japan Journal of Food Engineering   25 ( 3 )   59 - 64   2024.9

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    Authorship：Lead author,　Corresponding author  

    DOI： 10.11301/jsfe.24648

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  • Preparation of composite monoliths of quaternized chitosan and diatom earth for protein separation Reviewed

    Takaaki Tanaka, Yuna Tomita, Koki Honda, Marino Fujisawa, Akihito Ochiai

    Journal of Separation Science   46 ( 2 )   2200638 - 2200638   2023.1

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/jssc.202200638

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jssc.202200638

  • Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes Reviewed

    T. Tanaka, Y. Takai, A. Nagase, K. Teraguchi, H. Minbu, A. Ochiai, I. Kimura, M. Taniguchi

    Heliyon   5   e01490-1 - e01490-22   2019.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.heliyon.2019.e01490

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  • Poly(L-lactic acid) depth filter membrane prepared by nonsolvent-induced phase separation with the aid of a nonionic surfactant Reviewed

    H. Minbu, H. Mizuno, Y. Shibuya, A. Ochiai, M. Taniguchi, T. Tanaka

    Journal of Chemical Engineering of Japan   52 ( 1 )   75 - 82   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1252/jcej.18we084

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  • Preparation of poly(L-lactic acid) microfiltration membranes by a nonsolvent-induced phase separation method with the aid of surfactants Reviewed

    Hiromi Minbu, Akihito Ochiai, Tomoyuki Kawase, Masayuki Taniguchi, Douglas R. Lloyd, Takaaki Tanaka

    JOURNAL OF MEMBRANE SCIENCE   479   85 - 94   2015.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.memsci.2015.01.021

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  • Effects of SARS‑CoV‑2 mRNA vaccines on platelet polyphosphate levels and inflammation: A pilot study Reviewed

    Takashi Uematsu, Atsushi Sato, Hachidai Aizawa, Tetsuhiro Tsujino, Taisuke Watanabe, Kazushige Isobe, Hideo Kawabata, Yutaka Kitamura, Takaaki Tanaka, Tomoyuki Kawase

    Biomedical Reports   16 ( 3 )   2022.2

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    Publishing type：Research paper (scientific journal)   Publisher：Spandidos Publications  

    DOI： 10.3892/br.2022.1504

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  • Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility Reviewed

    Nakamura, Masayuki, Aizawa, Hachidai, Kawabata, Hideo, Sato, Atsushi, Watanabe, Taisuke, Isobe, Kazushige, Kitamura, Yutaka, Tanaka, Takaaki, Kawase, Tomoyuki

    International Journal of Implant Dentistry   6   74-1 - 74-14   2020.11

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  • Preparation of Polyhydroxyalkanoate Microfiltration Membranes via Nonsolvent–induced Phase Separation Methods

    Kazuyoshi Tabata, Toshiki Shibuya, Kousuke Karakida, Akihito Ochiai, Masayuki Taniguchi, Takaaki Tanaka

    MEMBRANE   45 ( 6 )   315 - 324   2020.11

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Membrane Society of Japan  

    DOI： 10.5360/membrane.45.315

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  • Crystal structure of rice defensin OsAFP1 and molecular insight into lipid-binding Reviewed

    Akihito Ochiai, Kodai Ogawa, Minami Fukuda, Masami Suzuki, Kosuke Ito, Takaaki Tanaka, Yoshiyuki Sagehashi, Masayuki Taniguchi

    Journal of Bioscience and Bioengineering   130 ( 1 )   6 - 13   2020.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    Defensins are antibacterial peptides that function in the innate immune system. OsAFP1, a defensin identified from Oryza sativa (rice), exhibits antimicrobial activity against rice pathogens. Intriguingly, OsAFP1 was also shown to demonstrate potent antifungal activity against the human pathogenic fungus Candida albicans by inducing apoptosis in target cells, suggesting that OsAFP1 represents a potential new antibiotic candidate; however, further analyses, particularly at the structural level, are required to elucidate the mechanistic underpinnings of OsAFP1 antifungal activity. Here, we determined the three-dimensional structure of OsAFP1 using X-ray crystallography. OsAFP1 features the cysteine-stabilized αβ structure highly conserved in plant defensins and presents a dimeric structure that appears necessary for antifungal activity. Superimposition of the OsAFP1 structure with that of Nicotiana alata NaD1 complexed with phosphatidic acid indicated that the target molecule is likely trapped between the S2-S3 loops of each OsAFP1 dimer. In lipid-binding analyses performed using nitrocellulose membranes immobilized with various membrane lipid components, OsAFP1 was found to bind to phosphatidylinositols (PIPs) harboring phosphate groups, particularly PI(3)P. These results indicate that OsAFP1 exerts antifungal activity by binding to PI(3)P contained in the C. albicans cell membrane, thereby applying cellular stress and inducing apoptosis. Furthermore, the OsAFP1 structure and site-specific-mutation analyses revealed that Arg1, His2, Leu4, Arg9, and Phe10 play critical roles in OsAFP1 dimer formation. Thus, our study provides novel insights into the antifungal mechanism of OsAFP1.

    DOI： 10.1016/j.jbiosc.2020.02.011

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  • Identification of cationic peptides derived from low protein rice by-products and evaluation of their multifunctional activities. Reviewed

    Taniguchi M, Aida R, Saito K, Oya R, Ochiai A, Saitoh E, Tanaka T

    Journal of Bioscience and Bioengineering   129 ( 3 )   307 - 314   2020.3

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  • Identification and characterization of multifunctional cationic peptides from enzymatic hydrolysates of soybean proteins. Reviewed

    Masayuki Taniguchi, Ryousuke Aida, Kazuki Saito, Toyotaka Kikura, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    Journal of Bioscience and Bioengineering   129 ( 1 )   59 - 66   2020.1

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    In this study, we used the commercial soybean protein hydrolysate Hinute-DC6 as a novel starting material from which to purify and identify multifunctional cationic peptides. After fractionation, Hinute-DC6 was separated into 20 fractions with varying isoelectric points (pI) by ampholyte-free isoelectric focusing (autofocusing). Subsequently, we purified and identified the cationic peptides from fractions 19 and 20, which had pI values greater than 12, using reversed-phase high-performance liquid chromatography and matrix-assisted laser/desorption ionization-time-of-flight mass spectrometry. Of the 83 cationic peptides identified, 14 had high pI values and net charges greater than +2, and were chemically synthesized and assayed for various bioactivities, including hemolytic, antimicrobial, lipopolysaccharide (LPS)-neutralizing, and angiogenic activities. None of the 14 cationic peptides tested exhibited hemolytic activity toward mammalian red blood cells at concentrations up to 1000 μM. Five of the cationic peptides exhibited antimicrobial activities against at least one of four human-pathogenic microorganisms tested. In addition, in chromogenic LPS-neutralizing assays using Limulus amebocyte lysates, the 50% effective concentrations of these 14 peptides were between 0.069 and 5.2 μM. Tube-formation assays in human umbilical vein endothelial cells showed that each of the 14 cationic peptides exhibited significant angiogenic activities at 10 μM, with values similar to those of the positive control LL-37. Our results demonstrate that the 14 identified cationic peptides have multiple functions with negligible hemolytic activity. These data indicate that the cationic peptides isolated from Hinute-DC6 and fractions containing these cationic peptides have the potential to be used as multifunctional ingredients for healthcare applications.

    DOI： 10.1016/j.jbiosc.2019.06.016

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  • Wound healing activity and mechanism of action of antimicrobial and lipopolysaccharide-neutralizing peptides from enzymatic hydrolysates of rice bran proteins. Reviewed

    Masayuki Taniguchi, Kazuki Saito, Ryousuke Aida, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    Journal of Bioscience and Bioengineering   128 ( 2 )   142 - 148   2019.8

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    In our previous study, we identified multifunctional cationic peptides from enzymatic hydrolysates of rice bran proteins (RBPs) that have antimicrobial and lipopolysaccharide-neutralizing activities. In this study, we investigated the potential of the peptides RBP-LRR, RBP-EKL, and RBP-SSF to promote proliferation, angiogenesis (tube formation), and migration in human umbilical vein endothelial cells (HUVECs). To determine mechanisms of wound healing actions, angiogenic and migration-promoting activities of these peptides were evaluated following pretreatments of HUVECs with specific inhibitors. In these experiments, the cationic peptides RBP-LRR, RBP-EKL, and RBP-SSF induced cell proliferation at low concentrations of 0.1 μM or 1 μM. Moreover, the three cationic peptides had angiogenic activities at concentrations more than 1 μM in tube formation assays, and their effects were similar to those of LL-37. Subsequent scratch migration assays exhibited that RBP-LRR, RBP-EKL, and RBP-SSF promote wound closure at optimum concentrations of 10, 10, and 0.1 μM, respectively. In further studies, we performed tube formation assays using HUVECs pretreated with SU5416, which inhibits vascular endothelial growth factor (VEGF) receptors, and suggested the possibility that the three cationic peptides induce angiogenesis by activating VEGF receptors. In corresponding scratch migration assays using HUVECs, pretreatment with the proliferation inhibitor mitomycin C did not alter the effects of RBP-LRR and RBP-EKL, and significant contribution to wound closure were mediated by cell migration regardless of proliferation rates. In contrast, RBP-SSF contributed to wound closure exclusively by promoting cell proliferation. The present data indicate that RBP-LRR, RBP-EKL, and RBP-SSF are candidates for use as wound healing agents.

    DOI： 10.1016/j.jbiosc.2019.02.002

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  • Evidence for contamination of silica microparticles in advanced platelet-rich fibrin matrices prepared using silica-coated plastic tubes Reviewed

    T. Tsujino, A. Takahashi, S. Yamaguchi, T. Watanabe, K. Isobe, Y. Kitamura, T. Tanaka, K. Nakata, T. Kawase

    Biomedicines   7 ( 2 )   45-1 - 45-11   2019.6

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    DOI： 10.3390/biomedicines7020045

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  • Identification and characterization of multifunctional cationic peptides from traditional Japanese fermented soybean Natto extracts. Reviewed

    Masayuki Taniguchi, Ryousuke Aida, Kazuki Saito, Akihito Ochiai, Satoshi Takesono, Eiichi Saitoh, Takaaki Tanaka

    Journal of Bioscience and bioengineering   127 ( 4 )   472 - 478   2019.4

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    In this study, we investigated the lipopolysaccharide (LPS)-neutralizing and angiogenic activities of cationic peptides derived from the traditional Japanese fermented product Natto, which is made by fermenting cooked soybeans using Bacillus subtilis. Initially, we prepared 20 fractions of Natto extracts with various isoelectric points (pI's) using ampholyte-free isoelectric focusing (autofocusing). Cationic peptides were then purified from fractions 19 and 20, whose pH values were greater than 12, using reversed-phase high-performance liquid chromatography, and were identified using matrix-assisted laser/desorption ionization-time-of-flight mass spectroscopy. Among the 13 identified cationic peptides, seven (KFNKYGR, FPFPRPPHQK, GQSSRPQDRHQK, QRFDQRSPQ, ERQFPFPRPPHQK, GEIPRPRPRPQHPE, and EQPRPIPFPRPQPR) had pI's greater than 9.5, positive net charges, and differing molecular weights. These peptides were then chemically synthesized and applied to chromogenic LPS-neutralizing assays using Limulus amebocyte lysates, and 50% effective (neutralizing) concentrations of 2.6-5.5 μM were demonstrated. In addition, tube formation assays in human umbilical vein endothelial cells revealed angiogenic activities for all but one (GEIPRPRPRPQHPE) of these seven cationic peptides, with increases in relative tube lengths of 23-31% in the presence of peptides at 10 μM. Subsequent experiments showed negligible hemolytic activity of these peptides at concentrations of up to 500 μM in mammalian red blood cells. Collectively, these data demonstrate that six cationic peptides from Natto extracts, with the exception of GEIPRPRPRPQHPE, have LPS-neutralizing and angiogenic activities but do not induce hemolysis.

    DOI： 10.1016/j.jbiosc.2018.09.016

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  • Cationic peptides from enzymatic hydrolysates of soybean proteins exhibit LPS-neutralizing and angiogenic activities. Reviewed

    Masayuki Taniguchi, Yusuke Noda, Ryousuke Aida, Kazuki Saito, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    Journal of bioscience and bioengineering   127 ( 2 )   176 - 182   2019.2

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    In this study, we prepared fractions containing multifunctional cationic peptides by separating the commercial soybean protein hydrolysate Hinute-AM into 20 fractions. These fractions contained peptides with various isoelectric points (pI), as indicated by ampholyte-free isoelectric focusing (autofocusing). Thus, we purified and identified the cationic peptides from fractions 19 and 20, which had pH values greater than 10, using reversed-phase high-performance liquid chromatography and matrix-assisted laser/desorption ionization-time-of-flight mass spectroscopy. Among 19 identified cationic peptides, NKNAKPPSPR, PGKKNAIV, KSGPGMSPR, NVSKPPRVV, RKVGAGGRKPLG, and LPCVIGGVPKRV had high pI values and were included as chemically synthesized peptides in assays of various functions, including lipopolysaccharide (LPS)-neutralizing and angiogenic activities. Chromogenic LPS-neutralizing assays using Limulus amebocyte lysates showed that 50% effective concentrations of these six peptides were between 1.63 and 2.65 μM, and were higher than that (0.12 μM) of polymyxin B. Moreover, in tube-formation assays in human umbilical vein endothelial cells, all of the six cationic peptides except LPCVIGGVPKRV exhibited angiogenic activities similar to those of the positive control LL-37. In addition, the six identified cationic peptides had no hemolytic activity at concentrations up to 500 μM in mammalian red blood cells. Our results demonstrate that five of the identified cationic peptides, excluding LPCVIGGVPKRV, have multiple functions and little or no hemolytic activity. These data indicate that fractions containing cationic peptides from Hinute-AM have the potential to be used as dietary supplements and functional ingredients in food products.

    DOI： 10.1016/j.jbiosc.2018.07.013

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  • Spectrophotometric determination of platelet counts in platelet-rich plasma Reviewed

    Y. Kitamura, M. Suzuki, T. Tsukioka, K. Isobe, T. Tsujino, T. Watanabe, T. Watanabe, H. Okudera, K. Nakata, T. Tanaka, T. Kawase

    International Journal of Implant Dentistry   4   29-1 - 29-8   2018.10

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    DOI： 10.1186/s40729-018-0140-8

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  • Rice Defensin OsAFP1 is a New Drug Candidate against Human Pathogenic Fungi Reviewed

    Ochiai Akihito, Ogawa Kodai, Fukuda Minami, Ohori Masahiro, Kanaoka Takumi, Tanaka Takaaki, Taniguchi Masayuki, Sagehashi Yoshiyuki

    SCIENTIFIC REPORTS   8   2018.7

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  • The antimicrobial and anti-endotoxic peptide AmyI-1-18 from rice α-amylase and its [N3L] analog promote angiogenesis and cell migration. Reviewed International journal

    Masayuki Taniguchi, Akihito Ochiai, Toshiki Namae, Kazuki Saito, Tetsuo Kato, Eiichi Saitoh, Takaaki Tanaka

    Peptides   104   78 - 84   2018.6

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  • Platelet counts in insoluble platelet-rich fibrin clots: A direct method for accurate determination Reviewed

    Yutaka Kitamura, Taisuke Watanabe, Masayuki Nakamura, Kazushige Isobe, Hideo Kawabata, Kohya Uematsu, Kazuhiro Okuda, Koh Nakata, Takaaki Tanaka, Tomoyuki Kawase

    Frontiers in Bioengineering and Biotechnology   6   2018.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media S.A.  

    DOI： 10.3389/fbioe.2018.00004

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  • An updated proposal for terminology and classification of platelet-rich fibrin Reviewed

    Tomoyuki Kawase, Takaaki Tanaka

    REGENERATIVE THERAPY   7   80 - 81   2017.12

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  • Cationic peptides from peptic hydrolysates of rice endosperm protein exhibit antimicrobial, LPS-neutralizing, and angiogenic activities Reviewed

    Masayuki Taniguchi, Junya Kawabe, Ryu Toyoda, Toshiki Namae, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    PEPTIDES   97   70 - 78   2017.11

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    DOI： 10.1016/j.peptides.2017.09.019

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  • Identification and characterization of multifunctional cationic peptides derived from peptic hydrolysates of rice bran protein Reviewed

    Masayuki Taniguchi, Mitsuhiro Kameda, Toshiki Namae, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    JOURNAL OF FUNCTIONAL FOODS   34   287 - 296   2017.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jff.2017.04.046

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  • Identification and characterization of multifunctional cationic and amphipathic peptides from soybean proteins Reviewed

    Masayuki Taniguchi, Kengo Saito, Takafumi Nomoto, Toshiki Namae, Akihito Ochiai, Eiichi Saitoh, Takaaki Tanaka

    BIOPOLYMERS   108 ( 4 )   2017.7

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    DOI： 10.1002/bip.23023

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  • Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) Reviewed

    Isobe Kazushige, Watanebe Taisuke, Kawabata Hideo, Kitamura Yutaka, Okudera Toshimitsu, Okudera Hajime, Uematsu Kohya, Okuda Kazuhiro, Nakata Koh, Tanaka Takaaki, Kawase Tomoyuki

    INTERNATIONAL JOURNAL OF IMPLANT DENTISTRY   3   2017.5

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  • Platelet-rich fibrin prepared from stored whole-blood samples Reviewed

    Isobe Kazushige, Suzuki Masashi, Watanabe Taisuke, Kitamura Yutaka, Suzuki Taiji, Kawabata Hideo, Nakamura Masayuki, Okudera Toshimitsu, Okudera Hajime, Uematsu Kohya, Nakata Koh, Tanaka Takaaki, Kawase Tomoyuki

    INTERNATIONAL JOURNAL OF IMPLANT DENTISTRY   3   2017.3

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  • Effects of arginine and leucine substitutions on anti-endotoxic activities andmechanisms of action of cationic and amphipathic antimicrobial octadecapeptide from rice alpha-amylase Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Ryu Toyoda, Teppei Sato, Eiichi Saitoh, Tetsuo Kato, Takaaki Tanaka

    JOURNAL OF PEPTIDE SCIENCE   23 ( 3 )   252 - 260   2017.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/psc.2983

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  • Antimicrobial activity against <i>Porphyromonas gingivalis</i> and mechanism of action of the cationic octadecapeptide Amyl-1-18 and its amino acid-substituted analogs Reviewed

    M. Taniguchi, A. Ochiai, K. Takahashi, S. Nakamichi, T. Nomoto, E. Saitoh, T. Kato, T. Tanaka

    Journal of Bioscience and Bioengineering   122 ( 6 )   652 - 659   2016.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Society for Biotechnology  

    DOI： 10.1016/j.jbiosc.2016.05.008

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    Other Link： http://search.jamas.or.jp/link/ui/2017254575

  • Preparation of a poly(L-lactic acid) membrane scaffold with open finger-like pores prepared by a nonsolvent-induced phase separation method with the aid of a surfactant Reviewed

    H. Minbu, T. Kawase, A. Ochiai, M. Taniguchi, T. Tanaka

    MEMBRANE   41 ( 6 )   304 - 310   2016.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：THE MEMBRANE SOCIETY OF JAPAN  

    Here, we prepared a poly(L–lactic acid) (PLLA) microporous membrane with open finger–like pores as a scaffold for tissue engineering by a nonsolvent–induced phase separation method with the aid of a surfactant. The increase of surfactant content in the polymer solution caused the mold (glass plate) side of the membrane to adopt an indented structure with open finger–like pores to enhance the internal surface area. Osteoblast–like cells inoculated on the indented side grew on and in the PLLA membrane scaffold to reach 2 ～ 3 times higher cell density per unit apparent area compared to that attained in monolayer cultures. The cells on the membrane deposited calcium compounds by osteoinduction with ascorbic acid 2–phosphate, dexamethasone, and β– glycerophosphate. The PLLA membrane with open finger–like pores will likely be useful as scaffolds to support the implantation of osteogenic cells in bone tissue engineering.

    DOI： 10.5360/membrane.41.304

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    Other Link： http://search.jamas.or.jp/link/ui/2017289540

  • Rice Bran Protein as a Potent Source of Antimelanogenic Peptides with Tyrosinase Inhibitory Activity Reviewed

    Akihito Ochiai, Seiya Tanaka, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF NATURAL PRODUCTS   79 ( 10 )   2545 - 2551   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.jnatprod.6b00449

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  • AmyI-1-18, a cationic alpha-helical antimicrobial octadecapeptide derived from alpha-amylase in rice, inhibits the translation and folding processes in a protein synthesis system Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Shun Fukuda, Teppei Sato, Eiichi Saitoh, Tetsuo Kato, Takaaki Tanakal

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   122 ( 4 )   385 - 392   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2016.03.004

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  • New tyrosinase inhibitory decapeptide: Molecular insights into the role of tyrosine residues Reviewed

    Akihito Ochiai, Seiya Tanaka, Yuta Imai, Hisashi Yoshida, Takumi Kanaoka, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   121 ( 6 )   607 - 613   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2015.10.010

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  • Pyrrhocoricin, a proline-rich antimicrobial peptide derived from insect, inhibits the translation process in the cell-free Escherichia coli protein synthesis system Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Hiroshi Kondo, Shun Fukuda, Yohei Ishiyama, Eiichi Saitoh, Tetsuo Kato, Takaaki Tanaka

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   121 ( 5 )   591 - 598   2016.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2015.09.002

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  • Effect of alanine, leucine, and arginine substitution on antimicrobial activity against candida albicans and action mechanism of a cationic octadecapeptide derived from -amylase of rice Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Kiyoshi Takahashi, Shun-ichi Nakamichi, Takafumi Nomoto, Eiichi Saitoh, Tetsuo Kato, Takaaki Tanaka

    BIOPOLYMERS   106 ( 2 )   219 - 229   2016.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bip.22817

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  • Endotoxin-neutralizing activity and mechanism of action of a cationic alpha-helical antimicrobial octadecapeptide derived from alpha-amylase of rice Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Kenta Matsushima, Koji Tajima, Tetsuo Kato, Eiichi Saitoh, Takaaki Tanaka

    PEPTIDES   75   101 - 108   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.peptides.2015.11.006

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  • The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation Reviewed

    Tomoyuki Kawase, Mana Kamiya, Mito Kobayashi, Takaaki Tanaka, Kazuhiro Okuda, Larry F. Wolff, Hiromasa Yoshie

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   103 ( 4 )   825 - 831   2015.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jbm.b.33262

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  • Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro Reviewed

    Tomoyuki Kawase, Takaaki Tanaka, Kazuhiro Okuda, Makoto Tsuchimochi, Masafumi Oda, Toshiaki Hara

    CYTOSKELETON   72 ( 5 )   246 - 255   2015.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/cm.21221

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  • Antimicrobial Activity and Mechanism of Action of a Novel Cationic alpha-Helical Octadecapeptide Derived From alpha-Amylase of Rice Reviewed

    Masayuki Taniguchi, Akihito Ochiai, Kiyoshi Takahashi, Shun-ichi Nakamichi, Takafumi Nomoto, Eiichi Saitoh, Tetsuo Kato, Takaaki Tanaka

    BIOPOLYMERS   104 ( 2 )   73 - 83   2015.3

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  • Contribution of Cationic Amino Acids toward the Inhibition of Arg-Specific Cysteine Proteinase (Arg-gingipain) by the Antimicrobial Dodecapeptide, CL(14-25), from Rice Protein Reviewed

    Masayuki Taniguchi, Yoshiyasu Matsuhashi, Takako K. Abe, Yohei Ishiyama, Eiichi Saitoh, Tetsuo Kato, Akihito Ochiai, Takaaki Tanaka

    BIOPOLYMERS   102 ( 5 )   379 - 389   2014.9

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  • An atmospheric-pressure plasma-treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal-derived osteoblastic cells Reviewed

    Tomoyuki Kawase, Takaaki Tanaka, Hiromi Minbu, Mana Kamiya, Masafumi Oda, Toshiaki Hara

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   102 ( 6 )   1289 - 1296   2014.8

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  • Crystal structure of alpha-amylase from Oryza sativa: molecular insights into enzyme activity and thermostability Reviewed

    Akihito Ochiai, Hiroshi Sugai, Kazuki Harada, Seiya Tanaka, Yohei Ishiyama, Kosuke Ito, Takaaki Tanaka, Toshio Uchiumi, Masayuki Taniguchi, Toshiaki Mitsui

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 6 )   989 - 997   2014.6

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    DOI： 10.1080/09168451.2014.917261

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  • alpha-Amylase is a potential growth inhibitor of Porphyromonas gingivalis, a periodontal pathogenic bacterium Reviewed

    A. Ochiai, K. Harada, K. Hashimoto, K. Shibata, Y. Ishiyama, T. Mitsui, T. Tanaka, M. Taniguchi

    JOURNAL OF PERIODONTAL RESEARCH   49 ( 1 )   62 - 68   2014.2

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  • Effect of Substituting Arginine and Lysine with Alanine on Antimicrobial Activity and the Mechanism of Action of a Cationic Dodecapeptide (CL(14-25)), a Partial Sequence of Cyanate Lyase from Rice Reviewed

    Masayuki Taniguchi, Nobuteru Takahashi, Tomohiro Takayanagi, Atsuo Ikeda, Yohei Ishiyama, Eiichi Saitoh, Tetsuo Kato, Akihito Ochiai, Takaaki Tanaka

    BIOPOLYMERS   102 ( 1 )   58 - 68   2014.1

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  • Biomechanical evaluation by AFM of cultured human cell-multilayered periosteal sheets Reviewed

    Makoto Horimizu, Tomoyuki Kawase, Takaaki Tanaka, Kazuhiro Okuda, Masaki Nagata, Douglas M. Burns, Hiromasa Yoshie

    Micron   48   1 - 10   2013.5

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    DOI： 10.1016/j.micron.2013.02.001

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  • Antimicrobial activity and mechanism of action of a novel cationic alpha-helical dodecapeptide, a partial sequence of cyanate lyase from rice Reviewed

    Norihiro Takei, Nobuteru Takahashi, Tomohiro Takayanagi, Atsuo Ikeda, Kenji Hashimoto, Masahiro Takagi, Tsutomu Hamada, Eiichi Saitoh, Akihito Ochiai, Takaaki Tanaka, Masayuki Taniguchi

    PEPTIDES   42   55 - 62   2013.4

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    DOI： 10.1016/j.peptides.2012.12.015

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  • Microporous membranes of PLLA/PCL blends for periosteal tissue scaffold Reviewed

    Tomoaki Kouya, Shin-ichiro Tada, Hiromi Minbu, Yu Nakajima, Makoto Horimizu, Tomoyuki Kawase, Douglas R. Lloyd, Takaaki Tanaka

    MATERIALS LETTERS   95   103 - 106   2013.3

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  • Evaluation of positive interaction for cell growth between Bifidobacterium adolescentis and Propionibacterium freudenreichii using a co-cultivation system with two microfiltration modules Reviewed

    Tomoaki Kouya, Yohei Ishiyama, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   115 ( 2 )   189 - 192   2013.2

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  • Antimicrobial activity and mechanism of action of a novel cationic β-helical octadecapeptide derived from heat shock protein 70 of rice Reviewed

    Masayuki Taniguchi, Atsuo Ikeda, Shun-Ichi Nakamichi, Yohei Ishiyama, Eiichi Saitoh, Tetsuo Kato, Akihito Ochiai, Takaaki Tanaka

    Peptides   48   147 - 155   2013

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    DOI： 10.1016/j.peptides.2013.08.011

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  • Evaluation of positive interactions for cell growth between bifidobacterial strains and propionibacterium freudenreichii by using a co-cultivation system Reviewed

    Tomoaki Kouya, Yohei Ishiyama, Takaaki Tanaka, Masayuki Taniguchi

    Journal of Chemical Engineering of Japan   46 ( 3 )   226 - 229   2013

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    DOI： 10.1252/jcej.12we231

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  • Formation of depth filter microfiltration membranes of poly(L-lactic acid) via phase separation Reviewed

    Takaaki Tanaka, Takayuki Nishimoto, Kazuhiro Tsukamoto, Masaharu Yoshida, Tomoaki Kouya, Masayuki Taniguchi, Douglas R. Lloyd

    JOURNAL OF MEMBRANE SCIENCE   396   101 - 109   2012.4

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  • An osteogenic grafting complex combining human periosteal sheets with a porous poly(L-lactic acid) membrane scaffold: Biocompatibility, biodegradability, and cell fate in vivo Reviewed

    Tomoyuki Kawase, Takaaki Tanaka, Takayuki Nishimoto, Kazuhiro Okuda, Masaki Nagata, Douglas M. Burns, Hiromasa Yoshie

    JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS   27 ( 2 )   107 - 121   2012.3

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  • Depth Filtration of Cell Suspensions of Agglomerated Streptobacteria by Biodegradable Membranes Reviewed

    Masaharu Yoshida, Masayuki Taniguchi, Takaaki Tanaka

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   45 ( 9 )   778 - 784   2012

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  • Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules Reviewed

    Tomoyuki Kawase, Takaaki Tanaka, Takayuki Nishimoto, Kazuhiro Okuda, Masaki Nagata, Douglas M. Burns, Hiromasa Yoshie

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   98A ( 1 )   100 - 113   2011.7

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  • Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying Reviewed

    Takaaki Tanaka, Masatou Ueno, Youhei Watanabe, Tomoaki Kouya, Masayuki Taniguchi, Douglas R. Lloyd

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   44 ( 7 )   467 - 475   2011

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  • Growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria Reviewed

    Masayuki Taniguchi, Yohei Ishiyama, Takeomi Takata, Toshihiro Nakanishi, Mitsuoki Kaneoke, Ken-ichi Watanabe, Fujitoshi Yanagida, Yi-sheng Chen, Tomoaki Kouya, Takaaki Tanaka

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   109 ( 6 )   570 - 575   2010.6

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  • Mechanical properties of microporous foams of biodegradable plastic Reviewed

    Takaaki Tanaka, Takashi Aoki, Tomoaki Kouya, Masayuki Taniguchi, Wataru Ogawa, Yuuji Tanabe, Douglas R. Lloyd

    DESALINATION AND WATER TREATMENT   17 ( 1-3 )   37 - 44   2010.5

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    DOI： 10.5004/dwt.2010.1696

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  • Filtration characteristics of carbon nanotubes and preparation of buckypapers Reviewed

    Takaaki Tanaka

    DESALINATION AND WATER TREATMENT   17 ( 1-3 )   193 - 198   2010.5

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    DOI： 10.5004/dwt.2010.1717

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  • Formation of microporous membranes of poly(1,4-butylene succinate) via nonsolvent and thermally induced phase separation Reviewed

    Takaaki Tanaka, Masaki Takahashi, Shigeko Kawaguchi, Takeru Hashimoto, Hiroshi Saitoh, Tomoaki Kouya, Masayuki Taniguchi, Douglas R. Lloyd

    DESALINATION AND WATER TREATMENT   17 ( 1-3 )   176 - 182   2010.5

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  • Enzymatic oxidative polymerization of methoxyphenols Reviewed

    Takaaki Tanaka, Masafumi Takahashi, Hiroyuki Hagino, Shin-Ichi Nudejima, Hisaki Usui, Tomoyuki Fujii, Masayuki Taniguchi

    CHEMICAL ENGINEERING SCIENCE   65 ( 1 )   569 - 573   2010.1

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    DOI： 10.1016/j.ces.2009.05.041

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  • Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw. Reviewed

    M. Taniguchi, D. Takahashi, D. Watanabe, K. Sakai, K. Hoshino, T. Kouya, T. Tanaka

    J. Biosci. Bioeng.   110 ( 4 )   449 - 452   2010

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    The effects of steam explosion (1.5MPa, 1min) on the treatment of rice straw with Pleurotus ostreatus were evaluated in terms of the change in composition of the components and the susceptibility to enzymatic hydrolysis. When rice straw was pretreated with a steam explosion prior to biological treatment, the treatment time required for obtaining a 33% net glucose yield was reduced to 36 days from 60 days. The reduction is probably due to loosening of networks of Klason lignin with sugar moieties and partial collapse of the structure during the biological treatment.

    DOI： 10.1016/j.jbiosc.2010.04.014

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  • Microfiltration of Bacterial Cell Suspensions with an Asymmetric Depth Filter Reviewed

    Masaharu Yoshida, Masayuki Taniguchi, Takaaki Tanaka

    KAGAKU KOGAKU RONBUNSHU   36 ( 5 )   494 - 500   2010

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    DOI： 10.1252/kakoronbunshu.36.494

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  • Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw Reviewed

    Masayuki Taniguchi, Daisuke Takahashi, Daisuke Watanabe, Kenji Sakai, Kazuhiro Hoshino, Tomoaki Kouya, Takaaki Tanaka

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   43 ( 4 )   401 - 405   2010

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  • Depth filter membranes of biodegradable biomass plastics

    Takaaki Tanaka, Takayuki Nishimoto, Tomoaki Kouya, Masayuki Taniguchi, Douglas R. Lloyd

    AIChE Annual Meeting, Conference Proceedings   2010

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  • Growth-inhibitory activity of antimicrobial peptides from rice against oral pathogenic bacteria and clarification of their action mechanism Reviewed

    Tomoaki Kouya, Yusuke Koiso, Mayumi Taiyouji, Sadami Ohtsubo, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S141 - S141   2009.11

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  • Growth-inhibition of Lactobacillus hilgardii, a bacterium related to hiochi, in the mizu-koji process by bacteriocins from lactic acid bacteria. Reviewed

    Y. Ishiyama, T. Takata, T. Nakanishi, M. Kaneoke, K. Watanabe, F. Yanagida, Y. Chen, T. Kouya, T. Tanaka, M. Taniguchi

    Jpn. J. Food Eng.   10 ( 1 )   45 - 53   2009

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  • Microfiltration of Incompressible Particle Suspension with an Asymmetric Depth Filter Reviewed

    Takaaki Tanaka, Tomoya Mimura, Mitsuo Koga, Masaharu Yoshida, Masayuki Taniguchi, Kazuhiro Nakanishi

    KAGAKU KOGAKU RONBUNSHU   35 ( 1 )   81 - 86   2009

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  • Enzymatic treatment of estrogens and estrogen glucuronide Reviewed

    Takaaki Tanaka, Toshiyuki Tamura, Yuuichi Ishizaki, Akito Kawasaki, Tomokazu Kawase, Masahiro Teraguchi, Masayuki Taniguchi

    JOURNAL OF ENVIRONMENTAL SCIENCES   21 ( 6 )   731 - 735   2009

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    DOI： 10.1016/S1001-0742(08)62332-3

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  • Microporous foams of polymer blends of poly(L-lactic acid) and poly(epsilon-caprolactone) Reviewed

    Takaaki Tanaka, Satoko Eguchi, Hiroshi Saitoh, Masayuki Taniguchi, Douglas R. Lloyd

    DESALINATION   234 ( 1-3 )   175 - 183   2008.12

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    DOI： 10.1016/j.desal.2007.09.084

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  • Production of bacteriocin by Staphylococcus sp. NPSI 38 in koji extract medium with rice protein hydrolyzate and its growth-inhibitory activity against hiochi-bacteria Reviewed

    Yohei Ishiyama, Takeomi Takata, Toshihiro Nakanishi, Naomi Watanabe, Mistuoki Kaneoke, Ken-Ichi Watanabe, Takaaki Tanaka, Masayuki Taniguchi

    Food Science and Technology Research   14 ( 3 )   239 - 248   2008.5

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    DOI： 10.3136/fstr.14.239

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  • Production of bacteriocin by Staphylococcus sp NPSI 38 in koji extract medium with rice protein hydrolyzate and its growth-inhibitory activity against hiochi-bacteria Reviewed

    Yohei Ishiyama, Takeomi Takata, Toshihiro Nakanishi, Naomi Watanabe, Mistuoki Kaneoke, Ken-ichi Watanabe, Takaaki Tanaka, Masayuki Taniguchi

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   14 ( 3 )   239 - 248   2008.5

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  • Production of extracellular bifidogenic growth stimulator (BGS) from Propionibacterium shermanii using a bioreactor system with a microfiltration module and an on-line controller for lactic acid concentration Reviewed

    Tomoaki Kouya, Kazuhiro Tobita, Masahito Horiuchi, Eri Nakayama, Hiroyoshi Deguchi, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 3 )   184 - 191   2008.3

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  • Production of Bifidogenic Growth Stimulator by Co-cultivation of Propionibacterium shermanii and Lactobacillus Strain Using Lactose as a Carbon Source Reviewed

    Tomoaki Kouya, Masahiro Horiuchi, Kazuhiro Tobita, Katsuhiro Misawa, Eri Nakayama, Hiroyoshi Deguchi, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   41 ( 6 )   492 - 496   2008

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    DOI： 10.1252/jcej.07we309

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  • Production of extracellular bifidogenic growth stimulator by anaerobic and, aerobic cultivations of several propionibacterial strains Reviewed

    Tomoaki Kouya, Katsuhiro Misawa, Masahito Horiuchi, Eri Nakayama, Hiroyoshi Deguchi, Takaaki Tanaka, Masayuki Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   103 ( 5 )   464 - 471   2007.5

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    DOI： 10.1263/jbb.103.464

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  • Production of laccase by membrane-surface liquid culture of Trametes versicolor using a poly(L-lactic acid) membrane Reviewed

    Takaaki Tanaka, Satoko Eguchi, Takashi Aoki, Toshiyuki Tamura, Hiroshi Saitoh, Masayuki Taniguchi, Hitomi Ohara, Kazuhiro Nakanishi, Douglas R. Lloyd

    BIOCHEMICAL ENGINEERING JOURNAL   33 ( 2 )   188 - 191   2007.2

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    DOI： 10.1016/j.bej.2006.10.002

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  • Microfiltration membrane of polymer blend of poly(L-lactic acid) and poly(epsilon-caprolactone) Reviewed

    T Tanaka, T Tsuchiya, H Takahashi, T Masayuki, DR Lloyd

    DESALINATION   193 ( 1-3 )   367 - 374   2006.5

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    DOI： 10.1016/j.desal.2005.06.068

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  • Formation of biodegradable polyesters membranes via thermally induced phase separation Reviewed

    T Tanaka, T Tsuchiya, H Takahashi, M Taniguchi, H Ohara, DR Lloyd

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   39 ( 2 )   144 - 153   2006.2

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    DOI： 10.1252/jcej.39.144

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  • Production of D-lactic acid from defatted rice bran by simultaneous saccharification and fermentation Reviewed

    T Tanaka, M Hoshina, S Tanabe, K Sakai, S Ohtsubo, M Taniguchi

    BIORESOURCE TECHNOLOGY   97 ( 2 )   211 - 217   2006.1

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    DOI： 10.1016/j.biortech.2005.02.025

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  • Production of L-lactic acid by simultaneous saccharification and fermentation using unsterilized defatted rice bran as a carbon source and nutrient components Reviewed

    M Taniguchi, M Hoshina, S Tanabe, Y Higuchi, K Sakai, S Ohtsubo, K Hoshino, T Tanaka

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   11 ( 4 )   400 - 406   2005.12

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    DOI： 10.3136/fstr.11.400

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  • Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw Reviewed

    M Taniguchi, H Suzuki, D Watanabe, K Sakai, K Hoshino, T Tanaka

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   100 ( 6 )   637 - 643   2005.12

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    DOI： 10.1263/jbb.100.637

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  • Production of L-lactic acid from a mixture of xylose and glucose by co-cultivation of lactic acid bacteria Reviewed

    M Taniguchi, T Tokunaga, K Horiuchi, K Hoshino, K Sakai, T Tanaka

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   66 ( 2 )   160 - 165   2004.12

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    DOI： 10.1007/s00253-004-1671-x

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  • Formation of poly(L-lactic acid) microfiltration membranes via thermally induced phase separation Reviewed

    T Tanaka, DR Lloyd

    JOURNAL OF MEMBRANE SCIENCE   238 ( 1-2 )   65 - 73   2004.7

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    DOI： 10.1016/j.memsci.2004.03.020

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  • Clarification of interactions among microorganisms and development of co-culture system for production of useful substances Reviewed

    M Taniguchi, T Tanaka

    RECENT PROGRESS OF BIOCHEMICAL AND BIOMEDICAL ENGINEERING IN JAPAN I   90   35 - 62   2004

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  • Treatment of nonylphenol with laccase in a rotating reactor Reviewed

    T Tanaka, M Nose, A Endo, T Fujii, M Taniguchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   96 ( 6 )   541 - 546   2003.12

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    DOI： 10.1016/S1389-1723(04)70147-4

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  • Enzymatic oxidative polymerization of 4-chloroguaiacol by laccase Reviewed

    T Tanaka, M Yamamoto, M Takahashi, T Fujii, M Taniguchi

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   36 ( 9 )   1101 - 1106   2003.9

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    DOI： 10.1252/jcej.36.1101

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  • Relationship between cake structure and membrane pore size in crossflow filtration of microbial cell suspension containing fine particles Reviewed

    T Tanaka, Y Yamagiwa, T Nagano, M Taniguchi, K Nakanishi

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   34 ( 12 )   1524 - 1531   2001.12

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    DOI： 10.1252/jcej.34.1524

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  • Treatment of Model Soils Contaminated with Phenolic Endocrine Disrupting Chemicals by Laccase from Trametes sp. in a Rotating Reactor Reviewed

    T. Tanaka, T. Tonosaki, M. Nose, N. Tomidokoro, N. Kadomura, T. Fujii, M. Taniguchi

    Journal of Bioscience and Bioengineering   92 ( 4 )   312 - 316   2001

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    IF1.749

    DOI： 10.1263/jbb.92.312

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  • Kefiran Production by Lactobacillus kefiranofaciens under the Culture Conditions Established by Mimicking the Existence and Activities of Yeast in Kefir Grains Reviewed

    Masayuki Taniguchi, Masashi Nomura, Takahiro Itaya, Takaaki Tanaka

    Food Science and Technology Research   7 ( 4 )   333 - 337   2001

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    DOI： 10.3136/fstr.7.333

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  • Enzymatic degradation of alkylphenols, bisphenol A, synthetic estrogen and phthalic ester Reviewed

    T Tanaka, K Yamada, T Tonosaki, T Konishi, H Goto, M Taniguchi

    WATER SCIENCE AND TECHNOLOGY   42 ( 7-8 )   89 - 95   2000

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  • Production of a mixture of antimicrobial organic acids from lactose by co-culture of Bifidobacterium longum and Propionibacterium freudenreichii Reviewed

    M Taniguchi, H Nakazawa, O Takeda, T Kaneko, K Hoshino, T Tanaka

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   62 ( 8 )   1522 - 1527   1998.8

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    DOI： 10.1271/bbb.62.1522

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  • Formation of the gel layer of polymers and its effect on the permeation flux in crossflow filtration of Corynebacterium glutamicum broth Reviewed

    T Tanaka, K Usui, K Nakanishi

    SEPARATION SCIENCE AND TECHNOLOGY   33 ( 5 )   707 - 722   1998

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    DOI： 10.1080/01496399808544784

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  • Characteristics in crossflow filtration using different yeast suspensions Reviewed

    T Tanaka, S Tsuneyoshi, W Kitazawa, K Nakanishi

    SEPARATION SCIENCE AND TECHNOLOGY   32 ( 11 )   1885 - 1898   1997

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    DOI： 10.1080/01496399708000743

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  • Filtration behaviors of rod-shaped bacterial broths in unsteady-state phase of cross-flow filtration Reviewed

    T Tanaka, K Usui, K Kouda, K Nakanishi

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   29 ( 6 )   973 - 981   1996.12

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    DOI： 10.1252/jcej.29.973

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  • CROSS-FLOW FILTRATION OF BAKERS-YEAST WITH PERIODICAL STOPPING OF PERMEATION FLOW AND BUBBLING Reviewed

    T TANAKA, H ITOH, K ITOH, K NAKANISHI, T KUME, R MATSUNO

    BIOTECHNOLOGY AND BIOENGINEERING   47 ( 3 )   401 - 404   1995.8

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  • SYNTHESIS OF ASPARTAME PRECURSOR WITH AN IMMOBILIZED THERMOLYSIN IN MIXED ORGANIC-SOLVENTS Reviewed

    M MIYANAGA, T TANAKA, T SAKIYAMA, K NAKANISHI

    BIOTECHNOLOGY AND BIOENGINEERING   46 ( 6 )   631 - 635   1995.6

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  • PRODUCTION OF KOJIC ACID FROM ASPERGILLUS-ORYZAE VAR ORYZAE BY MEMBRANE-SURFACE LIQUID CULTURE Reviewed

    A OGAWA, Y MORITA, T TANAKA, T SAKIYAMA, K NAKANISHI

    BIOTECHNOLOGY TECHNIQUES   9 ( 2 )   153 - 156   1995.2

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    DOI： 10.1007/BF00224417

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  • PRODUCTION OF KOJIC ACID BY MEMBRANE-SURFACE LIQUID CULTURE OF ASPERGILLUS-ORYZAE NRRL484 Reviewed

    A OGAWA, Y WAKISAKA, T TANAKA, T SAKIYAMA, K NAKANISHI

    JOURNAL OF FERMENTATION AND BIOENGINEERING   80 ( 1 )   41 - 45   1995

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0922-338X(95)98174-J

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  • PRODUCTION OF NEUTRAL PROTEASE BY MEMBRANE-SURFACE LIQUID CULTURE OF ASPERGILLUS-ORYZAE IAM2704 Reviewed

    A OGAWA, A YASUHARA, T TANAKA, T SAKIYAMA, K NAKANISHI

    JOURNAL OF FERMENTATION AND BIOENGINEERING   80 ( 1 )   35 - 40   1995

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    DOI： 10.1016/0922-338X(95)98173-I

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  • SYNTHESIS OF ASPARTAME PRECURSOR WITH AN IMMOBILIZED THERMOLYSIN IN TERT-AMYL ALCOHOL Reviewed

    T NAGAYASU, M MIYANAGA, T TANAKA, T SAKIYAMA, K NAKANISHI

    BIOTECHNOLOGY AND BIOENGINEERING   43 ( 11 )   1118 - 1123   1994.5

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    DOI： 10.1002/bit.260431116

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  • CROSS-FLOW FILTRATION OF YEAST BROTH CULTIVATED IN MOLASSES Reviewed

    T TANAKA, R KAMIMURA, R FUJIWARA, K NAKANISHI

    BIOTECHNOLOGY AND BIOENGINEERING   43 ( 11 )   1094 - 1101   1994.5

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    DOI： 10.1002/bit.260431113

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  • SYNTHESIS OF DIPEPTIDE PRECURSORS WITH AN IMMOBILIZED THERMOLYSIN IN ETHYL-ACETATE Reviewed

    T NAGAYASU, M MIYANAGA, T TANAKA, T SAKIYAMA, K NAKANISHI

    BIOTECHNOLOGY AND BIOENGINEERING   43 ( 11 )   1108 - 1117   1994.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bit.260431115

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  • PRODUCTION OF NEUTRAL PROTEASE FROM ASPERGILLUS-ORYZAE BY A NOVEL CULTIVATION METHOD ON A MICROPOROUS MEMBRANE

    A YASUHARA, A OGAWA, T TANAKA, T SAKIYAMA, K NAKANISHI

    BIOTECHNOLOGY TECHNIQUES   8 ( 4 )   249 - 254   1994.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/BF00155416

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  • SHEAR-INDUCED ARRANGEMENT OF CELLS IN CAKE DURING CROSS-FLOW FILTRATION OF ESCHERICHIA-COLI-CELLS Reviewed

    T TANAKA, K ABE, K NAKANISHI

    BIOTECHNOLOGY TECHNIQUES   8 ( 1 )   57 - 60   1994.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/BF00207634

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  • FILTRATION CHARACTERISTICS AND STRUCTURE OF CAKE IN CROSS-FLOW FILTRATION OF BACTERIAL SUSPENSION

    T TANAKA, K ABE, H ASAKAWA, H YOSHIDA, K NAKANISHI

    JOURNAL OF FERMENTATION AND BIOENGINEERING   78 ( 6 )   455 - 461   1994

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    DOI： 10.1016/0922-338X(94)90046-9

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  • Factors affecting the performance of crossflow filtration of yeast cell suspension Reviewed

    T Tanaka, K Nakanishi

    DEVELOPMENTS IN FOOD ENGINEERING, PTS 1 AND 2   653 - 655   1994

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  • IMPROVEMENT OF PERFORMANCE FOR CROSS-FLOW MEMBRANE FILTRATION OF PULLULAN BROTH Reviewed

    H YAMASAKI, MS LEE, T TANAKA, K NAKANISHI

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   39 ( 1 )   21 - 25   1993.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/bf00166842

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  • CHARACTERISTICS OF CROSS-FLOW FILTRATION OF PULLULAN BROTH Reviewed

    H YAMASAKI, MS LEE, T TANAKA, K NAKANISHI

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   39 ( 1 )   26 - 30   1993.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/bf00166843

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  • FACTORS AFFECTING THE PERFORMANCE OF CROSS-FLOW FILTRATION OF YEAST-CELL SUSPENSION Reviewed

    T TANAKA, R KAMIMURA, K ITOH, K NAKANISHI, R MATSUNO

    BIOTECHNOLOGY AND BIOENGINEERING   41 ( 6 )   617 - 624   1993.3

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    DOI： 10.1002/bit.260410604

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  • SOME FACTORS AFFECTING THE FORMATION OF GAMMA-CYCLODEXTRIN USING CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS SP AL-6 Reviewed

    K TOMITA, T TANAKA, Y FUJITA, K NAKANISHI

    JOURNAL OF FERMENTATION AND BIOENGINEERING   70 ( 3 )   190 - 192   1990

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    DOI： 10.1016/0922-338X(90)90185-Y

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  • Enzymatic synthesis of L-glutamic acid dimer precursor with immobilized papain in an organic solvent Reviewed

    Takaaki Tanaka, Kenji Tomita, Kazuhiro Nakanishi

    Chemistry Express   5 ( 7 )   525 - 528   1990

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  • CONTINUOUS PRODUCTION OF L-SERINE BY IMMOBILIZED GROWING CORYNEBACTERIUM-GLYCINOPHILUM CELLS Reviewed

    T TANAKA, K YAMAMOTO, S TOWPRAYOON, H NAKAJIMA, K SONOMOTO, K YOKOZEKI, K KUBOTA, A TANAKA

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   30 ( 6 )   564 - 568   1989.6

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    DOI： 10.1007/BF00255360

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Books

  • Production, Forming/Molding, and High-Performance Technologies of Poly(lactic acid)

    Hiroshi Uyama, Yu-I Hsu, Takaaki Tanaka, And Others（ Role： Joint author）

    CMC Publishing Co.,Ltd.  2024.1  （ ISBN:9784781317649 ）

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  • 食品製造に役立つ食品工学事典

    日本食品工学会編, 田中孝明他（ Role： Joint author）

    2020.12 

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  • カーボンナノチューブの表面処理・分散技術と複合化事例

    技術情報協会編, 田中孝明（ Role： Joint author）

    技術情報協会  2019.12  （ ISBN:9784861047725 ）

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  • 生分解性プラスチックの素材・技術開発

    望月政嗣, 監修, 田中孝明, 他, 著（ Role： Joint author）

    エヌ・ティー・エス  2019.12  （ ISBN:9784860436230 ）

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  • 多孔質フィルム/膜の製造技術

    田中孝明, 佐光貞樹, 富康博, 川島敏行, 石塚紀生, 瀧健太郎, 串﨑義幸, 平井悠司, 下村政嗣, 横山英明, 早川晃鏡, 武野明義, 高橋紳矢, 松田裕行, 小野寺恒信, 笠井均, 及川英俊, 山田一博, 河野公一, 西川聡（ Role： Joint author）

    S&T出版  2016.6  （ ISBN:4907002564 ）

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  • 濾過スケールアップの正しい進め方と成功事例集

    技術情報協会編, 田中孝明

    技術情報協会  2014.8  （ ISBN:9784861045370 ）

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  • 化学便覧 応用化学編 第7版

    日本化学会編, 田中孝明

    丸善出版  2014.1  （ ISBN:9784621087596 ）

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  • 濾過プロセスの最適選定と効率改善

    情報機構編, 田中孝明

    情報機構  2010.10  （ ISBN:9784904080610 ）

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  • カーボンナノチューブの精製・前処理と分散・可溶化技術

    技術情報協会編, 田中孝明

    技術情報協会  2009.4  （ ISBN:9784861042829 ）

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  • 産業酵素の応用技術と最新動向

    井上國世監修, 田中孝明

    シーエムシー出版  2009.3  （ ISBN:9784781301082 ）

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  • バイオプロダクション

    化学工学会バイオ部会編, 田中孝明

    コロナ社  2006.5  （ ISBN:9784339067361 ）

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  • 食品工学ハンドブック

    日本食品工学会編, 田中孝明（ Role： Joint author）

    朝倉書店  2006.1  （ ISBN:9784254430912 ）

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  • 化学工学の進歩39，粒子・流体系フロンティア分離技術

    化学工学会編, 田中孝明

    槇書店  2005.10  （ ISBN:9784837506881 ）

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  • 生物工学ハンドブック

    日本生物工学会編, 田中孝明

    コロナ社  2005.6  （ ISBN:9784339067347 ）

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  • Clarification of Interactions among Microorganisms and Development of Co-culture System for Production of Useful Substances

    Adv. Biochem. Engin./Biotechnol., Springer  2004 

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  • Treatment of phenolic endocrine-disrupting chemicals by lignin-degrading enzymes.

    Wastewater Treatment Using Enzymes, Research Signpost, Kerala, India  2003 

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  • Permation behaviors in crossflow microfiltration of microbial suspensions containing fine particles and polymers.

    Proceedings of The First Conference of Aseanian Membrane Society, Tokyo, Japan  2002 

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  • Crossflow filtration of lactic acid bacteria: effects of cell shapes and dextran on permeation flux.

    Proceedings of The First Conference of Aseanian Membrane Society, Tokyo, Japan  2002 

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  • Formation and Structural Change of Cake during Crossflow Microfiltration of Microbial Cell Suspension Containing Fine Particles(jointly worked)

    Bioseparation Engineering  2000 

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  • On the stability of immobilized thermolysin in an organic solvent(jointly worked)

    Chemical Engineering Symposium Series 57, The Society of Chemical Engineers, Japan  1997 

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  • Development of membrane-surface liquid culture of molds(jointly worked)

    1995 

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  • Optimization for synthesis of aspartame precursor using an immobilized thermolysin in an organic solvent(jointly worked)

    Chemical Engineering Symposium Series 44, The Society of Chemical Engineers, Japan  1995 

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  • Crossflow filtration of microbial suspensions(jointly worked)

    Continental Press Singapore, Better living through innovative biochemical engineering  1994 

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  • 生物工学実験書

    日本生物工学会編, 田中孝明

    培風館  1992.10  （ ISBN:4563077178 ）

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  • Crossflow membrane filtration of highly viscous microbial broth(jointly worked)

    S. Furusaki, I. Endo, R. Matsuno, Springer Verlag Tokyo, Biochemical Engineering for 2001  1992 

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Industrial property rights

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Awards

  • Outstanding Paper Award, Membrane

    2021.6   The Membrane Society of Japan   Preparation of polyhydroxyalkanoate microfiltration membranes via nonsolvent–induced phase separation methods

    Kazuyoshi Tabata, Toshiki Shibuya, Kousuke Karakida, Akihito Ochiai, Masayuki Taniguchi, Takaaki Tanaka

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    Award type：Honored in official journal of a scientific society, scientific journal 

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  • The 25th Paper Award of The Society for Biotechnology, Japan

    2017.9   The Society for Biotechnology, Japan   AmyI-1–18, a cationic α-helical antimicrobial octadecapeptide derived from α-amylase in rice, inhibits the translation and folding processes in a protein synthesis system

    TANAKA Takaaki

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  • Outstanding Paper Award of MEMBRANE

    2017.5   The Membrane Society of Japan   Preparation of a poly(L-lactic acid) membrane scaffold with open finger-like pores prepared by a nonsolvent-induced phase separation method with the aid of a surfactant

    H. Minbu, T. Kawase, A. Ochiai, M. Taniguchi, T. Tanaka

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  • Award for Excellence to Authors Publishing in Bioscience, Biotechnology, and Biochemisry in 2014

    2015.3   The Japan Society for Bioscience, Biotechnology, and Agrochemistry   Crystal structure of α-amylase from Oryza sativa: molecular insights into enzyme activity and thermostability

    Akihito Ochiai, Hiroshi Sugai, Kazuki Harada, Seiya Tanaka, Yohei Ishiyama, Kosuke Ito, Takaaki Tanaka, Toshio Uchiumi, Masayuki Taniguchi, Toshiaki Mitsui

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  • Paper Award

    2010.8   Japan Society for Food Engineering   Growth-inhibition of Lactobacillus hilgardii, a bacterium related to hiochi, in the mizu-koji process by bacteriocins from lactic acid bacteria

    Y. Ishiyama, T. Takata, T. Nakanishi, M. Kaneoke, K. Watanabe, F. Yanagida, Y. Chen, T. Kouya, T. Tanaka, M. Taniguchi

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  • The 17th Paper Award of The Society for Biotechnology, Japan

    2009.9   The Society for Biotechnology, Japan   Production of extracellular bifidogenic growth stimulator (BGS) from Propionibacterium shermanii using a bioreactor system with a microfiltration module and an on-line controller for lactic acid concentration

    T. Kouya, K. Tobita, M. Horiuchi, E. Nakayama, H. Deguchi, T. Tanaka, M. Taniguchi

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  • Encouragement Award of Japan Society for Food Engineering

    2007.8   Japan Society for Food Engineering   Studies on Membrane Filtration of Food Microbial Cell Suspensions

    Takaaki Tanaka

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    Country：Japan

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Research Projects

  • アスリートの選手生命を救うＰＲＰ治療の確立に向けた基盤的研究

    Grant number：22K11496

    2022.4 - 2025.3

    System name：科学研究費助成事業

    Research category：基盤研究(C)

    Awarding organization：日本学術振興会

    牛木 隆志, 望月 友晴, 川瀬 知之, 田中 孝明

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • Development of mixed-mode monolith-type adsorption materials and their application to purification process of proteins and peptides

    Grant number：17K06921

    2017.4 - 2020.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    Tanaka Takaaki

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    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

    In this study mixed-mode monolith-type adsorption materials were developed to be used in separation and purification of proteins and peptides in biopharmaceuticals production. Porous monoliths were prepared from polymers (poly(methyl methacrylate) and poly(lactic acid)) via phase separation methods. Hydroxyapatite particles that have mixed-mode (multimodal) type adsorption property were mainly composited with the monoliths. The prepared monoliths have adsorption abilities toward model proteins. For example, bovine serum albumin and bovine gamma-globulin were separated by one of the developed monoliths.

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  • Control of the internal and surface structures of biodegradable porous membranes with the aid of surfactants and their application to bioprocess

    Grant number：24560957

    2012.4 - 2016.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TANAKA Takaaki, KAWASE Tomoyuki

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    Grant amount：\5330000 （ Direct Cost: \4100000 、 Indirect Cost：\1230000 ）

    Biodegradable separation membrane and scaffold materials have been developed by controlling their internal and surface structures with the aid of surfactants. Asymmetric microporous separation membranes of poly(L-lactic acid) with pores in the order of 0.1 μm were prepared from the polymer solutions containing surfactants with the hydrophilic-lipophilic balance (HLB) values from 15.3 to 16.7. Osteoblast-like cells grew in the 500 μm-thick biodegradable microporous membranes when the cells were seeded on the rough side of the membranes.

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  • Developments of scaffolds and processing technology to maximize the osteogenic potential of cultured periosteal sheets

    Grant number：24390443

    2012.4 - 2016.3

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    Kawase Tomoyuki, OKUDA KAZUHIRO, NAGATA MASAKI, TANAKA TAKAAKI

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    Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）

    The purpose of this project is to demonstrate ideal structure and stiffness of scaffolds for osteogenic cells and to develop ideal scaffolds with biodegradable polymer materials. Under regular culture conditions, human periosteal cells (PC) expressed integrin α1β1 and CD44 as major adhesion molecules. Atomospheric plasma treatment modified the titanium surface and facilitated their osteoblastic differentiation. We also found that human platelet-rich fibrin (PRF) extract can be substituted for FBS. As for scaffolding materials, we developed a combinational porous membrane made of polycaprolactone and hydroxyapatite and found its stiffness and microstructure appropriate for PCs.

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  • Control of composition and morphology of hollow hydroxyapatite microspheres and their application to dental implant materials releasing an antimicrobial agent

    Grant number：21560712

    2009 - 2011

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    KIMURA Isao, KANATANI Mitsugu, TANAKA Takaaki, AKAZAWA Toshiyuki

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    Hollow microspheres of calcium phosphate compounds(such as hydroxyapatite, calcium hydrogen phosphate, etc.) were prepared by using multiple emulsions. The composition, morphology, and crystallinity were controlled by the addition of one of a magnesium salt, a carbonate, and low molecular weight organic compounds. For obtaining fundamental knowledge to apply the microspheres to dental implants which release an antimicrobial agent, the conditions for making them adhere onto titanium were investigated. A model implant was fabricated through the following procedures : the formation of microsphere layers on the surface of a titanium screw, the impregnation of a dye solution as the model material of an antimicrobial agent into the microsphere layer, and the coating with biodegradable polymer, poly(lactic acid). With the aim of applying the microspheres to a root canal treatment, composite microcapsules of the microspheres with biodegradable polymer were prepared.

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  • Development of environmental-benign bioseparation membranes by hybridizing biodegradable porous materials and nano inorganic microcapsules

    Grant number：21560807

    2009 - 2011

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TANAKA Takaaki, KIMURA Isao

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    Environmentally benign separation membranes have developed by hybridizing microporous membranes of biodegradable plastics and hydroxyapatite nanoparticles. The depth filter microfiltration membranes of poly(L-lactic acid) developed by Dr. Tanaka, the head of the research project, retained the microcapsules comprised of hydroxyapatite nanoparticles developed by Dr. Kimura, the co-researcher. The hybrid membrane of the biodegradable membrane and hydroxyapatite microcapsules adsorbed bovine serum albumin, a model protein, at a low ionic strength and eluted at an elevated ionic strength.

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  • Control of internal structure of porous membranes of biodegradable polymer blends and its application to bioprocesses

    Grant number：18560748

    2006 - 2008

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TANAKA Takaaki, TANIGUCHI Masayuki

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    Grant amount：\3740000 （ Direct Cost: \3200000 、 Indirect Cost：\540000 ）

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  • Acceleration of bone formation on mandibular distraction osteogenesis

    Grant number：18592169

    2006 - 2008

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    KOBAYASHI Tadaharu, TANAKA Takaaki, IZUMI Naoya, EJIRI Sadakazu

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    Grant amount：\4160000 （ Direct Cost: \3500000 、 Indirect Cost：\660000 ）

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  • Development of porous membranes of poly (lactic acid) via thermally induced phase separation and its application to bioprocesses

    Grant number：15560673

    2003 - 2005

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TANAKA Takaaki, TANIGUCHI Masayuki, AOKI Toshiki, MATSUYMA Hideto

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    Grant amount：\3600000 （ Direct Cost: \3600000 ）

    Biodegradable polyesters, e.g., poly(_L-lactic acid), which are degraded by microorganisms in natural environment and degradable in human bodies, have recently received increased attention in bioengineering. In this study we have developed microporous membranes of biodegradable polyesters via thermally induced phase separation. At first effect of characteristics of polymers and conditions of phase separation (diluent, polymer concentration, and thermal history) on the growth of the droplet in phase separation and the structures and pore sizes of the formed porous materials. Then, we made microporous membranes of poly(_L-lactic acid), poly(ε-caprolactone), and their blends. The poly(_L-lactic acid) membrane showed the characteristics of a screen filter in the filtration of yeast cell suspensions. The membrane of a polymer blend of poly(_L-lactic acid) and poly(ε-caprolactone) showed that of a depth filter. The filtration flux with the latter membrane was much higher than that with the former. We also applied poly(_L-lactic acid) membrane to the production of an oxidase, laccase, by membrane-surface liquid culture of white rot fungus, Trametes versicolor. The membrane was stable at 28℃ and the fungus grown on the membrane secreted much more enzyme in the repeated use of membrane-surface liquid culture than in liquid surface culture. The porous biodegradable membranes would contribute to the development of sustainable bioindustry and biomedical engineering.

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  • 生分解性高分子の応用

    2002

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    Grant type：Competitive

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  • Formation and application of microporous biodegradable polyester membranes

    2002

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    Grant type：Competitive

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  • Mechanism and Optimization of Diffusion controlled enantioselective permeable membrane

    Grant number：13555213

    2001 - 2003

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    AOKI Toshiki, TACHIBANA Kozo, KANEKO Takashi, TANAKA Takaaki

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    Grant amount：\7200000 （ Direct Cost: \7200000 ）

    We have synthesized new well-defined polymers and developed optical resolution membranes which have high enantioselevtivity from these polymers. We proposed the separations occurred in diffusion process. However, the reason and detail were not clear and their permeation rates were low. In this study, several optimizations were carried out in these membrane process. For example, molecular structures, conditions in membrane preparation and permeation were examined.
    First, several new optically active polymers were synthesized. Especially, we have discovered new polymerization method to obtain chiral helical polymers. In addition, new chiral helical polymers were prepared by condensation polymerization.
    Secondly, we confirmed the separation of these membrane process occurred in diffusion process. In addition, chiral helicity was found to be effective for enantioselective permeation. In particular, we found that polymeric membranes whose chiral groups had been removed by reaction in membrane state showed enantioselective permeability.
    Lastly, thin membranes such as composite membranes and asymmetric membranes were prepared. High permeation was realized by using these thin membranes. Pervaporation was also tried to make the rate higher.
    In conclusion, we attained higher permeability and obtained many findings for practical use.

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  • Development of Nanobiore actor for Remediation of Foods and Environments

    Grant number：13558072

    2001 - 2002

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B)

    Awarding organization：Japan Society for the Promotion of Science

    FUJII Tomoyuki, MIYAWAKI Osato, MIYAKE Noriko, HATTORI Yoshio, TANAKA Takaaki

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    Grant amount：\6900000 （ Direct Cost: \6900000 ）

    A thin nanoporous silica membrane was prepared by the two-step sol-gel method for the purpose that the generation of an aggregate having the higher fractal dimension was suppressed in the intermediate process. It succeeded in the preparation of the thin silica film with nanospace of about 3.3nm. The model reaction in which sea sand was made to adsorb the alkylphenol was carried out, and it was found that adsorbing nonylphenol and octylphenol and bisphenol A are quickly decomposed by laccase. The decomposition of an artificial estrogen also confirmed being possible. Log P, which was an useful parameter when we evaluate the solvent property of supercritical carbon dioxide, was estimated. As the pressure of carbon dioxide was increase on the pressure of 3.0 11.8 MPa, log P tended to increase from 0.9 to 2.0. It was shown that the polarity had appeared in carbon dioxide under the supercritical state by the pressure, and that the polarity became small with the increase in the pressure. On the esterification reaction between stearic acid and ethanol using lipase, the reaction rate rose with the increase in the pressure, and it agreed with the increase tendency of log P approximately. It was shown from these results that the solvent property as a bulk had to be considered in addition to the interaction between solute and carbon dioxide, enzyme surface, wall surface, respectively, when it examined the enzyme reaction in nanospace by using supercritical carbon dioxide as a solvent.

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  • Conversion of Agricultural Wastes to Lactic Acid by a Combination of Pentose-fermentative Lactic Acid Bacteria and Reversibly Soluble-insoluble Enzymes

    Grant number：13836003

    2001 - 2002

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    TANIGUCHI Masayuki, TANAKA Takaaki

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    Grant amount：\3600000 （ Direct Cost: \3600000 ）

    1) Glucose and xylose were mainly obtained from delignified rice straw by hydrolysis using cellulase preparation. Glucose and arabinose were mainly obtained from defatted rice bran by hydrolysis using amylase and cellulase preparations.
    2) Lactobacillus vaccinostericus, Lactobacillus pentosus, Pediococcus pentosaceus, and Enterococcus casseliflavus were selected as a xylose-fermented lactic acid bacterium. More lactic acid was produced from MRS medium containing xylose by E. casseliflavus as compared with other lactic acid bacteria.
    3) The amount of lactic acid produced did not increase in a fermentation using MRS medium containing xylose and glucose as a carbon source and E. casseliflavus.
    4) The maintenance of pH at a low value in a simultaneous saccharification and fermentation of non-sterilized rice bran allowed the selective growth of Lactobacillus rhamnosus, a lactic acid bacterium added. About 30 g/L of L-lactic acid was produced from defatted rice bran in a simultaneous saccharification and fermentation in which the pH was kept at 4.5.

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  • 生体触媒を用いた内分泌攪乱化学物質分解システムの開発

    Grant number：12750698

    2000 - 2001

    System name：科学研究費助成事業

    Research category：奨励研究(A)

    Awarding organization：日本学術振興会

    田中 孝明

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    Grant amount：\1800000 （ Direct Cost: \1800000 ）

    平成12年度に引き続き,生体触媒による内分泌撹乱化学物質の分解処理システム(バイオレメディエーション・システム)の研究を進めた。平成12年度に開発した回転型バイオリアクターを用いて,砂質に付着したノニルフェノールの分解を行い,反応条件の検討とリスク削減の検証を行った。生体触媒としては白色腐朽菌Trametes sp.由来のラッカーゼを用い,モデル汚染底質としてノニルフェノールを付着させた海砂を用いた。反応装置としては5x5x6cmの正方柱型の反応容器内でモデル汚染底質を回転させる,回転型リアクターを用いた。リアクター中にて,主としてpH5, 30℃にて,15gのモデル汚染底質を酵素溶液45mlを用いて処理した。まず,リアクターの回転軸の角度が反応速度に与える影響について検討した。40-90度の範囲では角度を小さくすると処理速度が高くなったため,40度が最適であった。回転速度については0-45rpmの範囲では10rpmが最適であり,10rpm以上では,回転に要する動力は高くなるが,処理速度は高くならなかった。この傾向は酵素液量を変化させたときも同じであった。次に酵素濃度を変化させて処理反応を行ったところ,反応速度は酵素濃度の約1/2乗に比例してしか増加しなかった。砂粒子の表面上のノニルフェノールに対しては,酵素濃度を高めても酵素濃度当りの作用効率が低下すると考えられた。反応温度を高めると40℃までは反応速度が高められた。ただし,10℃においても反応時間を長くすると90%以上の処理が可能なことが示された。最後に,オスメダカを用いた暴露実験にて,処理後の砂質からの抽出物の毒性評価を,血中ビテロゲニン濃度を指標として行った。その結果,ラッカーゼ処理によりノニルフェノールの内分泌撹乱作用が大きく低下することが示された。

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  • 白色腐朽菌のリグニン分解酵素を用いた酸化重合反応による新規性分解性ポリマーの合成

    Grant number：11875175

    1999

    System name：科学研究費助成事業

    Research category：萌芽的研究

    Awarding organization：日本学術振興会

    谷口 正之, 田中 孝明

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    Grant amount：\2100000 （ Direct Cost: \2100000 ）

    本研究では,ラッカーゼを用いてフェノール類から生分解性ポリマーを合成することについて検討した。この研究において、以下の結果を得た。
    1)バイオリアクターを用いてCoriolus versicolorによるラッカーゼの効率的生産について検討した結果、固体培地の初期水分含量を70%とした培養14日目に最大酵素活性が得られた。
    2)固体培地から回収したラッカーゼを,硫安沈殿で濃縮した後、イオン交換クロマトグラフィーと疎水性クロマトグラフィーによって精製した結果、比活性が45倍となった高純度の酵素を得ることができた。
    3)精製したラッカーゼを用いて、フェノールおよびフェニルフェノールを基質モノマーとして重合反応を行った結果、いずれもpHが4.5のときにポリマーの収率が最大となった。
    4)フェニルフェノールを基質モノマーとして用いた場合には、反応溶媒にアセトンを添加することによってポリマー収率が向上した。アセトンを30%添加したときのポリマー収率は98%であった。
    5)フェノールを基質モノマーとして重合反応を行った場合に生成したポリマーは、ジメチルホルムアミドに溶解しない高分子量であった。一方、フェニルフェノールを重合した場合に生成したポリマーは、ジメチルホルムアミドに溶解し、その分子量は6,000-10,000であった。
    6)フェノールおよびフェニルフェノールの酵素酸化重合物の熱重量分析を行った結果、これらの重合物は340℃まで安定であった。

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  • Production of Bifidogenic Growth Stimulator and Its Application to Development of Probiotics

    Grant number：10555286

    1998 - 2000

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (B).

    Awarding organization：Japan Society for the Promotion of Science

    TANIGUCHI Masayuki, TANAKA Takaaki, IIDA Takamitsu, HOSHINO Kazuhiro, HOJO Kenichi, KANEKO Tsutomu

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    Grant amount：\12700000 （ Direct Cost: \12700000 ）

    1. The concentration and the productivity per time of bifidogenic growth stimulator were increased by using a co-culture system for a homolactic acid bacterium and a propionic acid bacterium, a culture system with microfiltration, a fed-batch culture system with feed back control of carbon source concentration, and a culture system with microfiltration and feed back control of carbon source concentration.
    2. A novel bioreactor system with two fermentors and microfiltration modules was developed for efficient production bifidobacterial cells by a co-culture of Propionibacterium freudenreichii and Bifidobacterium adolescentis as well as clarification of interactions between the two bacteria.
    3. The proposed co-culture system allowed the successful exchange of culture medium between two fermentors. When P.freudenreichii and B.adolescentis, B.longum, or B.breve were cultivated individually in the new co-culture system, the amount of bifidobacterial cells produced was 1.2-1.6 fold as high as that with batch culture.

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  • 残留農薬迅速分解のための固定化リグニン分解酵素バイオリアクターシステムの開発

    Grant number：09750869

    1997 - 1998

    System name：科学研究費助成事業

    Research category：奨励研究(A)

    Awarding organization：日本学術振興会

    田中 孝明

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    Grant amount：\2100000 （ Direct Cost: \2100000 ）

    リグニン分解酵素を用いた残留農薬の迅速分解を行うためのバイオリアクターシステムの開発を目的として、その基礎となる研究を行った。まず、酵素の生産条件を検討した。リグニン分解酵素生産菌としては4種類の担子菌(Corilus versicolor ,Pleurotus osteatus,Phanerochaete chrysosporium,Bjerkandera adusta)を用いた。固体培養と液体培養とでリグニン分解酵素(リグニンペルオキシダーゼ、マンガンペルオキシダーゼ、ラッカーゼ)の培養体積当たりの生産量を比較したところ、固体培養の酵素生産量が液体培養の場合と比較して10から100倍程度高かった。次に、固体培養の菌体外酵素液を用いて農薬の一種ペンタクロロフェノールの分解実験を行った。酵素としては主としてC.versicolorの菌体外酵素を用いた。0.1-1.0mMのペンタクロロフェノール溶液(10%メタノール含有)に酵素液を添加したところ、7日間で80%分解された。各種農薬の水への溶解度を検討したところ、極めて低かった。そこで河川底質に残留する農薬の酵素分解を検討した。汚染底質のモデル系として海砂にアルキルフェノールを吸着させたものを用いた。アルキルフェノールは多くの農薬と同じく疎水性であり、近年、一部の農薬と同様に内分泌撹乱化学物質として環境中での毒性が懸念されている。C.versicolorの菌体外酵素液でアルキルフェノールは分解された。また、酵素分解の至適pHは5であった。また、C.versicolorの菌体外酵素液から精製したラッカーゼでも海砂に吸着したアルキルフェノールが分解できた。ラッカーゼは反応に酸素を要求することから、汚染底質を撹拌しながら分解反応を行うバイオリアクターについて考察した。

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  • リグニン分解酵素の応用

    1996

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    Grant type：Competitive

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  • Application of lignin degrading enzymes

    1996

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    Grant type：Competitive

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  • DEVELOPMENT OF BIOREACTOR SYSTEM FOR OLIGOPEPTIDE SYNTHESIS COMBINED WITH A SIMULATED MOVING BED ADSORBER

    Grant number：07555563

    1995 - 1996

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for Scientific Research (A)

    Awarding organization：Japan Society for the Promotion of Science

    NAKANISHI Kazuhiro, SHIOTA Katashi, UTAGAWA Takashi, SIRAI Yoshihito, TANAKA Takaaki, SAKIYAMA Takaharu

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    Grant amount：\1700000 （ Direct Cost: \1700000 ）

    In this study, we aimed to construct an effective bioreactor for synthesis of aspartame precursor, as a model oligopeptide in an organic solvent, using thermolysin immobilized onto Amberlite XAD-7. First, we investigated the factors affecting the synthesis of the asparatme precursor using an immobilized enzyme. We studied a synthetic rate, particularly in a mixed organic solvent with ethyl acetate and tert-amyl alcohol. Using a mixed solvent of 33% tert-amyl alcohol and 67% ethyl acetate, the activity and stability of the immobilized thermolysin were high enough to conduct a continuous reaction. With increasing the content of tert-amyl alcohol, the stability of the immobilized enzyme was enhanced. The reason for the high activity and stability of the immobilized thermolysin in the presence of tert-amyl alcohol was studied from the viepoint of stability of calcium bound on the enzyme molecule, a stabilizing factor for thermolysin. An optimum water content for the synthesis of asparatame precursor was found to be 4-6 % in the mixed organic solvent. The concentration of L-phenyllalanine methyl ester (PheOMe) and N- (benzy loxy carbonyl) -L-as partic acid (Z-Asp) was optimized. The concentration of PheOMe was set to 200 mM and that of Z-Asp was 40 mM.We could also conduct a continuous reaction in a column reactor at 40ﾟC.The conversion at the outlet of the reactor with respect of Z-Asp was around 99% with a space velocity of 4.9 (1/h). Based on this result, we aimed to develop a bioreactor system combined with a simulated moving bed absorber. Namely, at the outlet of the bioreactor, the Z-Asp concentration was negligible low with asparatame precursor Z-Asp PheOMe of around 40 mM and PheOMe of 160 mM.Thus, we could recycle PheOMe to the bioreactor with continuous separation of PheOMe and Z-AspPheOMe using a simulated mooving bed adsorber. We found Dowex MWA-1 as a suitable adsorbent and analyzed adsorption/desorption behaviors of PheOMe and Z-AspPheOMe. We also calculated a separation performance for a simulated moving bed adosorber. We also showed a possible bioreactor system which could be used for an industrial production. Finally, we briefly compared an enzymatic synthesis and chemical synthesis.

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  • 食品加工プロセスにおける汚れの洗浄過程の解析とその効率化

    Grant number：06650859

    1994

    System name：科学研究費助成事業

    Research category：一般研究(C)

    Awarding organization：日本学術振興会

    中西 一弘, 田中 孝明, 崎山 高明

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    Grant amount：\2000000 （ Direct Cost: \2000000 ）

    食品製造工程やバイオプロセスにおいては、必ず装置表面に付着残存する汚れ成分を除去するための洗浄操作が実施されている。本研究では、洗浄操作の最適化を最終目的として、その基礎となる溶質のステンレス表面への付着・脱離現象の解明を行った。モデル汚れ成分として、β-ラクトグロブリンを取り上げた。溶質の付着特性と洗浄特性を精度よく調べるために、比表面積の大きなステンレス微粒子を用いた。タンパク質の付着量に及ぼす、付着時間、pH、温度、蛋白濃度の影響を調べた。温度が50℃以下では、その最大付着量は単分子層が形成される量にほぼ一致したが、β-ラクトグロブリンの変性温度である65℃前後から急激に増加し、単分子層の数倍程度となった。付着量はpHに強く依存した。β-ラクトグロブリンの等電点である5.1以下では付着量は急激に増加したが、これは負に帯電しているステンレス表面と正に帯電しているタンパク質との間の静電的相互作用によるものであることが判明した。また、未変性タンパク、予め変性したタンパク、及びSH基を修飾したタンパク質を用いて吸着実験を行った結果、β-ラクトグロブリンは、静電気的相互作用、及び疎水結合によりステンレス表面に付着し、その後タンパク質間でS-S結合の形成や交換反応により付着が進行することが明らかになった。
    洗浄特性に関しては、申請者らが開発した汚れの付着したステンレス粒子充填カラムを用いるステップ応答法を用いた。アルカリ洗浄と、プロテアーゼを用いる酵素洗浄に着目して比較検討した。酵素法では、アルカリ洗浄に比較してより低いpHで、洗浄が可能であり、しかも残存付着量も少ないことが判明した。また、アルカリ洗浄ではタンパク分子、あるいはその凝集体の形で、一方、酵素洗浄では分解ペプチドの形で、表面から脱離することが示唆された。

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  • 培地由来の微粒子を含む培養液のクロスフロー膜濾過に関する基礎的研究

    Grant number：05750719

    1993

    System name：科学研究費助成事業

    Research category：奨励研究(A)

    Awarding organization：日本学術振興会

    田中 孝明

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    Grant amount：\800000 （ Direct Cost: \800000 ）

    本研究は微粒子を含む培地で培養した菌体培養液のクロスフロー濾過特性を明らかにすることを目的として行った。平成5年度に得られた研究成果を以下に示す。
    (1)微粒子が混入した糖蜜を培地成分としてパン酵素を培養した培養液をクロスフロー濾過したときの濾過速度の変化を濾過期間を菌体層形成過程と微粒子層形成過程に分けることにより予測できることを示した。走査電子顕微鏡観察により微粒子の堆積を実証できた。クロスフロー速度、圧力などを変化させたときに濾過特性がどのように変化するかについてもまとめることができた。特にクロスフロー速度を高めた場合、通常の粒子懸濁液のクロスフロー濾過においては濾過速度が高くなるのに対して、微粒子混入系においては濾過速度が低下することを発見し、上述の2段階付着層形成過程モデルによりも導けることを示した。さらに逆洗を組み合わせたときの操作条件の最適化についてもまとめた。これらの結果は、国際食品工学会議(平成5年6月、千葉)、化学工学会秋季大会(平成5年10月、京都)にて発表した。
    (2)微粒子を含む培養液のモデル系として粒径の異なる高分子ラテックスを合成し、その濾過特性を調べた。デッドエンド型濾過特性およびクロスフロー濾過特性を調べた。これと菌体の濾過特性を組み合わせてクロスフロー濾過における濾過速度の粒径依存性についてまとめた。さらに菌体とラテックスの混合懸濁液を調製しクロスフロー濾過特性を検討した。

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  • 微生物菌体分離のためのクロスフロー瀘過法の高効率化

    Grant number：04203223

    1992

    System name：科学研究費助成事業

    Research category：重点領域研究

    Awarding organization：日本学術振興会

    中西 一弘, 田中 孝明, 富田 憲史

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    Grant amount：\2500000 （ Direct Cost: \2500000 ）

    バイオプロセスにおける除菌・集菌工程の効率化を目的として、クロスフロー膜瀘(Cross Flow Filtration;CFF)に着目して、その膜瀘過流束に及ぼす諸因子の解明を行うと共に、膜透過流束を高くするための方策を検討した。具体例として、(1)パン酵母培養液と、(2)プルラン培養液のCFFを取り上げた。
    YPD培地で純粋培養したパン酵母培養液のCFFにおいては、定常流束だけではなく、定常状態に達するまでの流束変化も瀘過理論から推測される結果と一致した。すなわち、クロスフロー膜瀘過時におけるケーク層の充填状態はデッドエンド型瀘過の場合と同様であることが判明した。膜透過流束に及ぼす膜間圧力差、循環流速、菌体濃度、膜の種類などの影響を調べた。循環流速と菌体濃度に関しては、モジュール内部が菌体により閉塞を起こす限界の値が存在することが判明した。
    天然培地であるモラッセスを用いて培養を行った場合の膜透過流束は、YPD培地で培養したパン酵母懸濁液の結果と比較して著しく低下した。この理由として、モラッセス中に存在する微粒子が菌体ケーク層表面上に薄い層をなして積もり、しかもこの薄層の比抵抗が菌体ケーク層よりも著しく高いことが明らかにされた。膜透過流束を高めるための方策を検討したところ、逆洗が最も効果的であった。逆洗による膜透過流束の回復は膜孔径に依存し、膜孔径が0.8μm以下の膜では殆どみられず、孔径が3μmあるいは5μmの膜では膜透過流束の回復は100%であった。逆洗を行うことにより、膜透過流束は5倍以上増加した。
    プルラン培養液のクロスフロー瀘過においては、菌体の形状が膜透過流束に大きく影響することが判明した。培養条件を工夫することにより膜透過流束は数十倍に増加した。

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  • Membrane filtration of microbial suspension

    1990

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    Grant type：Competitive

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  • 菌体懸濁液のクロスフロー濾過

    1990

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    Grant type：Competitive

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  • 微生物菌体分離のためのクロスフロ-濾過法の高効率化

    Grant number：02203238

    1990

    System name：科学研究費助成事業

    Research category：重点領域研究

    Awarding organization：日本学術振興会

    中西 一弘, 田中 孝明, 富田 憲史

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    Grant amount：\3000000 （ Direct Cost: \3000000 ）

    本年度は以下の三点の項目に関して検討を行った。
    (1)パン酵母懸濁液のクロスフロ-濾過機構の解明
    薄層流路型クロスフロ-濾過装置を用いて、パン酵母懸濁液のクロスフロ-濾過を行い、その膜透過流束に及ぼす諸因子を調べた。これらの実験結果から、膜透過機構に関して検討を行ったところ以下の知見が得られた。濾過開始直後においては、きわめて短期間であるが、菌体はDeadーend型の濾過の場合と同様な充填構造を取りながらも膜面上に堆積する。その後、菌体付着層表面近傍において、内部よりも密な構造の付着層が形成されることが明らかにされた。この理由として循環中に一部の菌体が変形しやすい構造に変質するためであることが示唆された。
    (2)膜透過流束を高くするための新規な操作方法の開発
    高い膜透過流束を得る目的で、種々の操作方法を検討した結果、濾口を周期的に開閉し、しかも濾口を閉じた時に気泡を供給すると、膜透過流束が数倍から10倍増加することが判明した。最適な条件では、2%のパン酵母懸濁液を10%に濃縮した場合の平均膜透過流束は3501/h・m^2に達した。
    (3)高粘性培養液のクロスフロ-濾過
    微生物菌体の中には、多糖など高分子物質を分泌するものが少なくない。これらの高分子物質は食品添加剤や生理活性物質としての理用が注目されている。本研究では、ケ-ススタディ-として、<Aureobasidium>___ー <pullulanns>___ーによる多糖プルランの発酵生産を行い、その高粘性培養液からの菌体の回収をクロスフロ-濾過法により行った。膜透過流束は低に値であったが、濾液は完全に清澄であり、プルランの膜透過率も90%と高く、クロスフロ-濾過法の有用性が示された。

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  • Syntheses of Umami Peptides with Immobilized Papain in Water-Immiscible Organic Solvent Systems

    Grant number：01560151

    1989 - 1990

    System name：Grants-in-Aid for Scientific Research

    Research category：Grant-in-Aid for General Scientific Research (C)

    Awarding organization：Japan Society for the Promotion of Science

    NAKANISHI Kazuhiro, TANAKA Takaaki

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    Grant amount：\2200000 （ Direct Cost: \2200000 ）

    Dipeptides such as L-glutamic acid dimer and L-glutamic acid dimer are known as a substance posessing umami (brothy) taste and a bitter taste masking effect. In this study, we attempted to synthesize precursors of four peptides, i. e., GluGlu, GluAsp, AspGlu, and AspAsp with an immobilized papain in organic solvent systems. As an acid component of a substrate, N-(benzyloxycarbonyl)-L-glutamic acid and N-(benzyloxycarbonyl)-L-aspartic acid, and as an amine component L-glutanic acid diethyl ester and L-aspartic acid diethyl ester were used. In most cases, we focussed on the synthesis of N-(benzyloxycarbonyl)-L-glutamyl-L-glutamic acid diethyl ester (Z-GluGlu(OEt)_2) at 30 ^ﾟC.
    The results obtained are Summarized as follows :
    1) The synthetic reaction did not take place in buffer solution.
    2) In the aqueous-organic biphasic reaction systems with ethyl acetate as an organic solvent, the maximum yield of Z-GluGlu (OEt) _2 reached about 70%.
    3) The enzyme was immobilized onto various porous supports. The enzyme was first adsorbed onto the support and then cross-linked with glutaraldehyde. The highest activity was obtained when the enzyme was adsorbed onto porous ceramic support (SM10-C1, Nippon Gaishi K. K.).
    4) The activity of immobilized enzyme depended strongly on the water content in an outer organic solvent. The highest activity was obtained at a water content of 2.5% with the enzyme immobilized onto SM10-C1.
    5) L-Glutamic acid diethyl ester, an amine component of the substrate was non-enzymatically coverted to L-pyroglutamic acid diethyl ester. To reduce the effect of non-enzymatic reaction, the increase of the concentration of the immobilized enzyme was found most effective. With 100 mM Z-Glu and 200 mM Glu (OEt) _2, and immobilized enzyme concentration of 8%, the yield became higher than 90%.
    6) The other three dipeptides precursors were also obtained with quite a high yield under the same reaction conditions as shown in (5).
    7) The products were identified by analyzing with nuclear magnetic resonance, mass spectrum, and infrared adsorption spectrum.

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  • 生物分離工学

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    Grant type：Competitive

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  • 酵素利用技術

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    Grant type：Competitive

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  • 膜分離技術(特に精密濾過)

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    Grant type：Competitive

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Teaching Experience

  • 国際工学概論

    2024
    Institution name：新潟大学

  • 統合化学入門

    2022
    Institution name：新潟大学

  • インターンシップ

    2022
    Institution name：新潟大学

  • 機能材料工学実験II

    2021
    Institution name：新潟大学

  • リメディアル演習

    2021
    Institution name：新潟大学

  • 生物機能材料科学

    2021
    Institution name：新潟大学

  • 技術英語II

    2020
    Institution name：新潟大学

  • 論文輪講II

    2020
    Institution name：新潟大学

  • 技術英語I

    2020
    Institution name：新潟大学

  • 論文輪講I

    2020
    Institution name：新潟大学

  • 先端科学技術総論

    2020
    Institution name：新潟大学

  • 材料科学実験II

    2019
    Institution name：新潟大学

  • 総合工学概論

    2017
    Institution name：新潟大学

  • 工学リテラシー入門(化学材料分野)

    2017
    Institution name：新潟大学

  • 材料科学概論

    2017
    Institution name：新潟大学

  • 基礎有機化学

    2016
    Institution name：新潟大学

  • 生物機能材料科学

    2015
    -
    2021
    Institution name：新潟大学

  • 材料生産システム博士特定研究Ⅱ

    2015
    Institution name：新潟大学

  • 中間発表

    2015
    Institution name：新潟大学

  • 材料生産システム博士セミナーⅡ

    2015
    Institution name：新潟大学

  • 情報機器操作入門

    2015
    Institution name：新潟大学

  • 外国語論文解説・討論Ⅱ

    2015
    Institution name：新潟大学

  • 機能材料化学概論

    2014
    -
    2016
    Institution name：新潟大学

  • 外国語論文解説・討論Ⅰ

    2014
    Institution name：新潟大学

  • 材料生産システム博士セミナーⅠ

    2014
    Institution name：新潟大学

  • 技術英語入門

    2014
    Institution name：新潟大学

  • 機能材料科学コース演習

    2014
    Institution name：新潟大学

  • 材料生産システム博士特定研究Ⅰ

    2014
    Institution name：新潟大学

  • 技術者倫理

    2014
    Institution name：新潟大学

  • 自然科学総論Ⅱ

    2013
    -
    2020
    Institution name：新潟大学

  • 機能材料科学文献詳読Ⅱ

    2012
    -
    2015
    Institution name：新潟大学

  • 研究発表演習・発表

    2012
    -
    2015
    Institution name：新潟大学

  • 材料生産システム特定研究Ⅱ

    2012
    -
    2015
    Institution name：新潟大学

  • 機能材料科学セミナーⅡ

    2012
    -
    2015
    Institution name：新潟大学

  • 材料生産システム特定研究Ⅰ

    2012
    -
    2015
    Institution name：新潟大学

  • 機能材料科学演習

    2012
    -
    2015
    Institution name：新潟大学

  • 機能材料科学文献詳読Ⅰ

    2012
    -
    2015
    Institution name：新潟大学

  • 機能材料科学セミナーⅠ

    2012
    -
    2015
    Institution name：新潟大学

  • 卒業研修

    2009
    Institution name：新潟大学

  • 機能材料科学総論Ⅱ

    2009
    Institution name：新潟大学

  • 工学リテラシー入門(機能材料工学科)

    2009
    -
    2016
    Institution name：新潟大学

  • 最先端技術を支える化学 II

    2008
    -
    2013
    Institution name：新潟大学

  • 機能材料工学実験IV

    2008
    -
    2010
    Institution name：新潟大学

  • スタディスキルズ(機能材料工学)

    2008
    Institution name：新潟大学

  • 卒業研究

    2007
    Institution name：新潟大学

  • 生物材料工学

    2007
    Institution name：新潟大学

  • 生物反応プロセス工学

    2007
    Institution name：新潟大学

  • 論文輪講

    2007
    -
    2022
    Institution name：新潟大学

  • 技術英語

    2007
    -
    2022
    Institution name：新潟大学

  • 機能材料工学実験III

    2007
    -
    2018
    Institution name：新潟大学

  • 基礎物理化学

    2007
    -
    2015
    Institution name：新潟大学

  • 化学実験

    2007
    -
    2014
    Institution name：新潟大学

  • 生物素材工学特論

    2007
    -
    2013
    Institution name：新潟大学

  • 卒業基礎研究

    2007
    -
    2008
    Institution name：新潟大学

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