Faculty Profiles - BABA Hiroshsi

Papers - BABA Hiroshsi

Division display 21 - 40 of about 225 ／ All the affair displays >>

Effects of the Kampo medicine Yokukansan for perioperative anxiety and postoperative pain in women undergoing breast surgery: A randomized, controlled trial Reviewed International journal

Moegi Tanaka, Tsunehiko Tanaka, Misako Takamatsu, Chieko Shibue, Yuriko Imao, Takako Ando, Hiroshi Baba, Yoshinori Kamiya

PLOS ONE 16 ( 11 ) e0260524 - e0260524 2021.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Public Library of Science (PLoS)

Yokukansan (YKS) is a traditional Japanese herbal (Kampo) medicine prescribed for anxiety. In this randomized controlled trial, we compared the subjective assessment of anxiety using questionnaires and its objective assessment using salivary alpha-amylase concentrations in YKS and control (CNT) groups of women undergoing breast surgery. The trial was registered at the University Hospital Medical Information Network Clinical Trials Registry (registration number: UMIN000028998), and the investigators were blinded to drug administration. One hundred patients who underwent breast cancer surgery were allocated to either the YKS or the CNT group. Finally, 35 and 42 patients in the YKS and CNT groups were analyzed, respectively. The YKS group received two 2.5 g doses of the medication before sleeping on the night before surgery and 2 h before inducing anesthesia, while the CNT group did not receive medication preoperatively. Patients answered two questionnaires, the Hospital Anxiety and Depression Scale and the State-Trait Anxiety Inventory, pre-and postoperatively as subjective anxiety assessments. As an objective anxiety indicator, salivary alpha-amylase levels were measured the day before, directly before, and the day after surgery (T3). In the YKS group, salivary alpha-amylase scores directly before operation were significantly lower than those on the day before surgery and at one day postoperatively (F [2,150] = 3.76, p = 0.03). Moreover, the Hospital Anxiety and Depression Scale-Anxiety and State-Trait Anxiety Inventory-Trait scores were significantly more improved postoperatively in the YKS group than in the CNT group (difference in Hospital Anxiety and Depression Scale-Anxiety: YKS, mean -2.77, 95% confidence interval [-1.48 –-4.06], p <0.001, and CNT, -1.43 [-0.25–-2.61], p = 0.011; and difference in State-Trait Anxiety Inventory: YKS group, -4.23 [-6.95–-1.51], p = 0.0004; and CNT group, 0.12 [-2.36–2.60], p = 0.92). No side effects were associated with YKS. YKS may reduce perioperative anxiety in patients undergoing surface surgery.

File： Tanaka M(PlosOne,2021).pdf

DOI： 10.1371/journal.pone.0260524

PubMed

researchmap

Epidural Administration of Ropivacaine Reduces the Amplitude of Transcranial Electrical Motor–Evoked Potentials Reviewed International journal

Kenta Furutani, Toshiyuki Tobita, Hideaki Ishii, Hiroyuki Deguchi, Yusuke Mitsuma, Yoshinori Kamiya, Hiroshi Baba

Anesthesia & Analgesia 132 ( 4 ) 1092 - 1100 2021.10

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Ovid Technologies (Wolters Kluwer Health)

BACKGROUND: An epidurally administered local anesthetic acts primarily on the epidural nerve roots and can act directly on the spinal cord through the dural sleeve. We hypothesized that epidurally administered ropivacaine would reduce the amplitude of transcranial electrical motor-evoked potentials by blocking nerve conduction in the spinal cord. Therefore, we conducted a double-blind, randomized, controlled trial. METHODS: Thirty adult patients who underwent lung surgery were randomly allocated to 1 of 3 groups, based on the ropivacaine concentration: the 0.2% group, the 0.375% group, and the 0.75% group. The attending anesthesiologists, neurophysiologists, and patients were blinded to the allocation. The epidural catheter was inserted at the T5-6 or T6-7 interspace by a paramedian approach, using the loss of resistance technique with normal saline. General anesthesia was induced and maintained using propofol and remifentanil. Transcranial electrical motor-evoked potentials were elicited by a train of 5 pulses with an interstimulus interval of 2 milliseconds by using a constant-voltage stimulator and were recorded from the tibialis anterior muscle. Somatosensory-evoked potentials (SSEPs) were evoked by electrical tibial nerve stimulation at the popliteal fossa. After measuring the baseline values of these evoked potentials, 10 mL of epidural ropivacaine was administered at the 0.2%, 0.375%, or 0.75% concentration. The baseline amplitudes and latencies recorded before administering ropivacaine were defined as 100%. Our primary end point was the relative amplitude of the motor-evoked potentials at 60 minutes after the epidural administration of ropivacaine. We analyzed the amplitudes and latencies of these evoked potentials by using the Kruskal-Wallis test and used the Dunn multiple comparison test as the post hoc test for statistical analysis. RESULTS: The data are expressed as the median (interquartile range). Sixty minutes after epidurally administering ropivacaine, the motor-evoked potential amplitude was lower in the 0.75% group (7% [3%-18%], between-group difference P < .001) and in the 0.375% group (52% [43%-59%]) compared to that in the 0.2% group (96% [89%-105%]). The latency of SSEP was longer in the 0.75% group compared to that in the 0.2% group, but the amplitude was unaffected. CONCLUSIONS: Epidurally administered high-dose ropivacaine lowered the amplitude of motor-evoked potentials and prolonged the onset latencies of motor-evoked potentials and SSEPs compared to those in the low-dose group. High-dose ropivacaine can act on the motor pathway through the dura mater.

File： Furutani(A&A,2021).pdf

DOI： 10.1213/ane.0000000000005236

PubMed

researchmap

A Bolus Dose of Ketamine Reduces the Amplitude of the Transcranial Electrical Motor-evoked Potential: A Randomized, Double-blinded, Placebo-controlled Study. Reviewed International journal

Kenta Furutani, Hiroyuki Deguchi, Mari Matsuhashi, Yusuke Mitsuma, Yoshinori Kamiya, Hiroshi Baba

Journal of neurosurgical anesthesiology 33 ( 3 ) 230 - 238 2021.7

Authorship：Last author Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: A low-dose bolus or infusion of ketamine does not affect transcranial electrical motor-evoked potential (MEP) amplitude, but a dose ≥1 mg/kg may reduce MEP amplitude. We conducted a randomized, double-blinded, placebo-controlled study to evaluate the effect of ketamine (1 mg/kg) on transcranial electrical MEP. METHODS: Twenty female patients (aged 12 to 18 y) with adolescent idiopathic scoliosis scheduled to undergo posterior spinal fusion were randomly allocated to receive ketamine or saline. General anesthesia was induced and maintained with continuous infusions of propofol and remifentanil. MEP was elicited by supramaximal transcranial electrical stimulation. MEP recordings were obtained at baseline and then at 2, 4, 6, 8, and 10 minutes after administration of ketamine (1 mg/kg) or saline (0.1 ml/kg). The primary endpoint was the minimum relative MEP amplitude (peak-to-peak amplitude, % of baseline value) recorded from the left tibialis anterior muscle. The baseline amplitude recorded before test drug administration was defined as 100%. RESULTS: Medians (interquartile range) minimum MEP amplitudes in the left tibialis anterior muscle in the ketamine and saline groups were 26% (9% to 34%) and 87% (55% to 103%) of the baseline value, respectively (P<0.001). MEP amplitudes in other muscles were significantly reduced by ketamine. The suppressive effect of ketamine lasted for at least 10 minutes in each muscle. CONCLUSION: A 1-mg/kg bolus dose of ketamine can reduce MEP amplitude. Anesthesiologists should consider the dosage and timing of intravenous ketamine administration during MEP monitoring.

File： Furutani (2019).pdf

DOI： 10.1097/ANA.0000000000000653

PubMed

researchmap

成人ムコ多糖症II型患者の開心術における周術期気道管理 Reviewed

三ッ間祐介, 清水大喜, 松田敬一郎, 本田博之, 今井英一, 馬場洋

麻酔 70 ( 6 ) 602 - 605 2021.6

Authorship：Last author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：克誠堂出版(株)

成人ムコ多糖症II型患者では、周術期の気道・呼吸管理が問題になる。本症例は意識下、気管支鏡ガイド下に経口挿管を試みたが、ムコ多糖の蓄積による気道のねじれと狭窄のため困難であった。経鼻アプローチに変更すると、咽頭軸との角度が緩やかになり比較的容易に挿管できた。成人症例では経鼻挿管が有用である可能性が示唆された。(著者抄録)

File：成人ムコ多糖症（三ッ間,2021,麻酔）.pdf

researchmap

Other Link： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J01397&link_issn=&doc_id=20210601120006&doc_link_id=%2Fad3msuie%2F2021%2F007006%2F007%2F0602b0605%26dl%3D3&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fad3msuie%2F2021%2F007006%2F007%2F0602b0605%26dl%3D3&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_4.gif

Propofol reduces the amplitude of transcranial electrical motor-evoked potential without affecting spinal motor neurons: a prospective, single-arm, interventional study Reviewed

Hiroyuki Deguchi, Kenta Furutani, Yusuke Mitsuma, Yoshinori Kamiya, Hiroshi Baba

Journal of Anesthesia 35 ( 3 ) 434 - 441 2021.4

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

PURPOSE: Propofol inhibits the amplitudes of transcranial electrical motor-evoked potentials (TCE-MEP) in a dose-dependent manner. However, the mechanisms of this effect remain unknown. Hence, we investigated the spinal mechanisms of the inhibitory effect of propofol on TCE-MEP amplitudes by evaluating evoked electromyograms (H-reflex and F-wave) under general anesthesia. METHODS: We conducted a prospective, single-arm, interventional study including 15 patients scheduled for spine surgery under general anesthesia. Evoked electromyograms of the soleus muscle and TCE-MEPs were measured at three propofol concentrations using target-controlled infusion (TCI: 2.0, 3.0, and 4.0 µg/mL). The primary outcome measure was the left H-reflex amplitude during TCI of 4.0- compared to 2.0-µg/mL propofol administration. RESULTS: The median [interquartile range] amplitudes of the left H-reflex were 4.71 [3.42-6.60] and 5.6 [4.17-7.46] in the 4.0- and 2.0-μg/mL TCI groups (p = 0.4, Friedman test), respectively. There were no significant differences in the amplitudes of the right H-reflex and the bilateral F-wave among these groups. However, the TCE-MEP amplitudes significantly decreased with increased propofol concentrations (p < 0.001, Friedman test). CONCLUSION: Propofol did not affect the amplitudes of the H-reflex and the F-wave, whereas TCE-MEP amplitudes were reduced at higher propofol concentrations. These results suggested that propofol can suppress the TCE-MEP amplitude by inhibiting the supraspinal motor pathways more strongly than the excitability of the motor neurons in the spinal cord.

File： Deguchi2021_Article_PropofolReducesTheAmplitudeOfT.pdf

DOI： 10.1007/s00540-021-02927-7

PubMed

researchmap

Other Link： http://link.springer.com/article/10.1007/s00540-021-02927-7/fulltext.html

Low-dose droperidol suppresses transcranial electrical motor-evoked potential amplitude: a retrospective study Reviewed

Hiroyuki Deguchi, Kenta Furutani, Yusuke Mitsuma, Yoshinori Kamiya, Hiroshi Baba

Journal of Clinical Monitoring and Computing 35 ( 1 ) 175 - 181 2021.2

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

File： Deguchi(J Clin MonitComput.2021).pdf

DOI： 10.1007/s10877-020-00464-4

researchmap

Other Link： http://link.springer.com/article/10.1007/s10877-020-00464-4/fulltext.html

Downfolding of the epiglottis into the laryngeal inlet after tracheal intubation using the McGRATHTM MAC videolaryngoscope: a case report Reviewed

Haruno Soma, Kenta Furutani, Ayaka Hibino, Akinobu Hibino, Hiroshi Baba

JA Clinical Reports 6 ( 1 ) 2020.12

Quadratus lumborum block type 2 for pedicle groin flap analgesia: a case report Reviewed International journal

Tatsunori Watanabe, Koji Moriya, Hiroshi Baba

JA Clinical Reports 6 ( 1 ) 36 - 36 2020.12

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

File： Quadratus lumborum block(Watanabe,2020,JA CR).pdf

DOI： 10.1186/s40981-020-00342-7

PubMed

researchmap

Other Link： http://link.springer.com/article/10.1186/s40981-020-00342-7/fulltext.html

SUZYTM forceps facilitate nasogastric tube insertion under McGRATHTM MAC videolaryngoscopic guidance Reviewed International journal

Kenta Furutani, Tatsunori Watanabe, Keiichiro Matsuda, Yoshinori Kamiya, Hiroshi Baba

Medicine 99 ( 41 ) e22545 - e22545 2020.10

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Ovid Technologies (Wolters Kluwer Health)

BACKGROUND: Nasogastric tubes can be easily inserted in patients under general anesthesia. However, for difficult cases, insertion techniques that can be used in routine clinical practice are limited. SUZY forceps are designed for the removal of pharyngolaryngeal foreign bodies under guidance of a McGrath videolaryngoscope. We hypothesized that using SUZY forceps under McGrath videolaryngoscopic guidance may facilitate nasogastric tube insertion and tested this in a randomized controlled trial. METHODS: Adult patients who underwent gastrointestinal or hepato-pancreato-biliary surgery were randomly allocated to 2 groups; the SUZY group and the Magill group. Patients, nurses, and all clinical staff except for the attending anesthesiologist were blinded to group assignment throughout the study. After anesthesia induction, insertion of the nasogastric tube was performed by skilled anesthesiologists with either SUZY or Magill forceps according to group allocation under McGrath videolaryngoscopic guidance. The primary endpoint was insertion time which was defined as the time required to advance the nasogastric tube by 55 cm from the nostril. Secondary endpoints were the success rates of the nasogastric tube insertion, which were defined as a 55-cm advancement from the nostril at the 1st, 2nd, and 3rd attempt, proper insertion rate, the severity of pharyngolaryngeal complications, and hemodynamic parameters during nasogastric tube insertion. RESULTS: Sixty patients were randomized and none of these patients were excluded from the final analysis. The median [interquartile range] insertion time was 25 [18-33] seconds in the SUZY group, and 33 [21-54] seconds in the Magill group (P = .02). Success rates were not different between the groups (97% and 80% in the SUZY and Magill group at 1st attempt, respectively, P = .10). Both, the severity score of the mucosal injury and the severity of sore throat were higher in the Magill than in the SUZY group, whereas the degree of hoarseness did not differ between the 2 groups. Hemodynamic parameters were not significantly different between the groups. CONCLUSION: Using SUZY forceps under McGrath videolaryngoscopic guidance reduced the time required to insert a nasogastric tube and the severity of pharyngolaryngeal complications, when compared to using Magill forceps.

File： Furutani(2020,Medicine).pdf

DOI： 10.1097/md.0000000000022545

PubMed

researchmap

In vivoフラビン蛋白蛍光イメージング法を用いた末梢神経損傷後のマウスにおける大脳皮質一時体性感覚野と脊髄後角の経時的な神経活動の違い Reviewed

番場景子

134 ( 7 ) 226 - 235 2020.7

Efficacy of a novel urinary catheter for men with a local anesthetic injection port for catheter-related bladder discomfort: a randomized controlled study Reviewed

Hidekazu Imai, Yutaka Seino, Hiroshi Baba

Journal of Anesthesia 34 ( 5 ) 688 - 693 2020.6

Congenital Tracheal Aplasia Without Prenatal Diagnosis Masked by Maternal Obesity and Gestational Diabetes Reviewed International journal

Tomohiro Yamamoto, Miyuki Kurabe, Kensuke Matsumoto, Shunya Sugai, Hiroshi Baba

A & A Practice 14 ( 6 ) e01200 - e01200 2020.4

Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Ovid Technologies (Wolters Kluwer Health)

This case report describes a neonate with tracheal aplasia first diagnosed after birth due to the presentation of respiratory distress, absence of crying, and unsuccessful tracheal intubation. The most common finding with tracheal aplasia is polyhydramnios. However, diagnosis remains challenging in the prenatal period. In this case, maternal obesity and gestational diabetes made diagnosis more difficult. The only lifesaving treatment available is ventilation through esophageal intubation or tracheostomy. However, in some cases, tracheostomy is not an option.

File： Congenital Tracheal(Yamamoto,A&A,2020).pdf

DOI： 10.1213/xaa.0000000000001200

PubMed

researchmap

A case of ulnar neuritis with persistent elbow pain for nine years Reviewed

27 ( 1 ) 87 - 90 2020.2

難治性の直腸癌術後の肛門部痛に対して漢方治療が奏効した一症例 Reviewed

清水大喜, 渡部達範, 田中萌生, 内藤夏子, 花房友海, 安部達也, 馬場洋

慢性疼痛 38 ( 1 ) 203 - 205 2019.12

Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. Reviewed International journal

Onishi T, Watanabe T, Sasaki M, Kamiya Y, Horie M, Tsukano H, Hishida R, Kohno T, Takebayashi H, Baba H, Shibuki K

The Journal of physiology 597 ( 13 ) 3441 - 3455 2019.5

Language：English Publishing type：Doctoral thesis

KEY POINTS: Neuropathic pain spreads spatially beyond the injured sites, and the mechanism underlying the spread has been attributed to inflammation occurring in the spinal cord. However, the spatial spread of spinal/cortical potentiation induced by conduction block of the peripheral nerves can be observed prior to inflammation. In the present study, we found that spreading potentiation and hypersensitivity acutely induced by unilateral hindpaw ischaemia are nitric oxide (NO)-dependent and that NO is produced by ischaemia and quickly diffuses within the spinal cord. We also found that NO production induced by ischaemia is not observed in the presence of an antagonist for group II metabotropic glutamate receptors (mGluRs) and that neuronal NO synthase-positive dorsal horn neurons express group II mGluRs. These results suggest strongly that NO-mediated spreading potentiation in the spinal cord is one of the trigger mechanisms for neuropathic pain. ABSTRACT: Cortical/spinal responses to hindpaw stimulation are bilaterally potentiated by unilateral hindpaw ischaemia in mice. We tested the hypothesis that hindpaw ischaemia produces nitric oxide (NO), which diffuses in the spinal cord to induce spatially spreading potentiation. Using flavoprotein fluorescence imaging, we confirmed that the spreading potentiation in hindpaw responses was induced during ischaemia in the non-stimulated hindpaw. This spreading potentiation was blocked by spinal application of l-NAME, an inhibitor of NO synthase (NOS). Furthermore, no spreading potentiation was observed in neural NOS (nNOS) knockout mice. Spinal application of an NO donor was enough to induce cortical potentiation and mechanical hypersensitivity. The spatial distribution of NO during unilateral hindpaw ischaemia was visualized using 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM). An increase in fluorescence derived from the complex of DAF-FM with NO was observed on the ischaemic side of the spinal cord. A similar but smaller increase was also observed on the contralateral side. Somatosensory potentiation after hindpaw ischaemia is known to be inhibited by spinal application of LY354740, an agonist of group II metabotropic glutamate receptors (mGluRs). We confirmed that the spinal DAF-FM fluorescence increases during hindpaw ischaemia were not observed in the presence of LY354740. We also confirmed that approximately half of the nNOS-positive neurons in the superficial laminae of the dorsal horn expressed mGluR2 mRNA. These results suggest that disinhibition of mGluR2 produces NO which in turn induces a spreading potentiation in a wide area of the spinal cord. Such spreading, along with the consequent non-specific potentiation in the spinal cord, may trigger neuropathic pain.

File： Onishi(JP,2019).pdf

DOI： 10.1113/JP277615

PubMed

researchmap

Optimal Position of Inferior Vena Cava Cannula in Pediatric Cardiac Surgery: A Prospective, Randomized, Controlled, Double-Blind Study. Reviewed

Seino Y, Ohashi N, Imai H, Baba H

Journal of cardiothoracic and vascular anesthesia 33 ( 5 ) 1253 - 1259 2019.5

自然気管破裂患者の気管裂傷部に二腔チューブが迷入した1症例 Reviewed

清水大喜, 上村夏生, 清野豊, 馬場洋, 小池輝元, 土田正則

臨床麻酔 43 ( 5 ) 693 - 696 2019.5

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：真興交易(株)医書出版部

非常に稀な自然気管破裂の1症例を経験した。症例は85歳の女性で、高度肥満、閉塞性睡眠時無呼吸症候群、関節リウマチの現病歴があり、ステロイド治療中であった。外傷などの誘因なく背部痛、呼吸困難が出現し、気管支鏡で分岐部直上の気管破裂を認めた。手術のために挿管された二腔チューブが、気管裂傷部に迷入し、換気困難になった。体外式膜型人工肺を使用して、手術を完遂し、治療を継続したが死亡した。(著者抄録)

File：清水（臨床麻酔,2019）.pdf

researchmap

Neurosteroid dehydroepiandrosterone sulphate enhances pain transmission in rat spinal dorsal horn. Reviewed International journal

Yamamoto G, Kamiya Y, Sasaki M, Ikoma M, Baba H, Kohno T

British Journal of Anaesthesia 123 ( 2 ) e215 - e225 2019.4

Language：English Publishing type：Doctoral thesis

BACKGROUND: The neurosteroid dehydroepiandrosterone sulphate (DHEAS) activates the sigma-1 receptor, inhibits gamma-aminobutyric acid A (GABAA) and glycine receptors, and induces hyperalgesic effects. Although its effects have been studied in various tissues of the nervous system, its synaptic mechanisms in nociceptive pathways remain to be elucidated. METHODS: The threshold of mechanical hypersensitivity and spontaneous pain behaviour was assessed using the von Frey test in adult male Wistar rats after intrathecal administration of DHEAS. We also investigated the effects of DHEAS on synaptic transmission in the spinal dorsal horn using slice patch-clamp electrophysiology. RESULTS: Intrathecally administered DHEAS elicited dose-dependent mechanical hyperalgesia and spontaneous pain behaviours (withdrawal threshold: saline; 51.0 [20.1] g, 3 μg DHEAS; 14.0 [7.8] g, P<0.01, 10 μg DHEAS; 6.9 [5.2] g, 15 min after administration, P<0.001). DHEAS at 100 μM increased the frequency of miniature postsynaptic currents in the rat dorsal spinal horn; this increase was extracellular Ca2+-dependent but not sigma-1 and N-methyl-d-aspartate receptor-dependent. DHEAS suppressed the frequency of miniature inhibitory postsynaptic currents in a GABAA receptor- and sigma-1 receptor-dependent manner. CONCLUSIONS: These results suggest that DHEAS participates in the pathophysiology of nociceptive synaptic transmission in the spinal cord by potentiation of glutamate release and inhibition of the GABAA receptor.

File： Yamamoto G(BJA,2019).pdf

DOI： 10.1016/j.bja.2019.03.026

PubMed

researchmap

Mechanisms of noradrenergic modulation of synaptic transmission and neuronal excitability in ventral horn neurons of the rat spinal cord. Reviewed International journal

Shoji H, Ohashi M, Hirano T, Watanabe K, Endo N, Baba H

Neuroscience 408 161 - 176 2019.4

Authorship：Last author Language：English Publishing type：Research paper (scientific journal)

Noradrenaline (NA) modulates the spinal motor networks for locomotion and facilitates neuroplasticity, possibly assisting neuronal network activation and neuroplasticity in the recovery phase of spinal cord injuries. However, neither the effects nor the mechanisms of NA on synaptic transmission and neuronal excitability in spinal ventral horn (VH) neurons are well characterized, especially in rats aged 7 postnatal days or older. To gain insight into NA regulation of VH neuronal activity, we used a whole-cell patch-clamp approach in late neonatal rats (postnatal day 7-15). In voltage-clamp recordings at -70 mV, NA increased the frequency and amplitude of excitatory postsynaptic currents via the activation of somatic α1- and β-adrenoceptors of presynaptic neurons. Moreover, NA induced an inward current through the activation of postsynapticα1- and β-adrenoceptors. At a holding potential of 0 mV, NA also increased frequency and amplitude of both GABAergic and glycinergic inhibitory postsynaptic currents via the activation of somatic adrenoceptors in presynaptic neurons. In current-clamp recordings, NA depolarized resting membrane potentials and increased the firing frequency of action potentials in VH neurons, indicating that it enhances the excitability of these neurons. Our findings provide new insights that establish NA-based pharmacological therapy as an effective method to activate neuronal networks of the spinal VH in the recovery phase of spinal cord injuries.

File： Neuroscience 論文.pdf

DOI： 10.1016/j.neuroscience.2019.03.026

PubMed

researchmap

Action of Norepinephrine on Lamina X of the Spinal Cord. Reviewed International journal

Ohashi N, Ohashi M, Baba H

Neuroscience 408 214 - 225 2019.4

Language：English Publishing type：Research paper (scientific journal)

Lamina X is localized in the spinal cord within the region surrounding the central canal and receives descending projections from the supraspinal nuclei. Norepinephrine (NE) is a neurotransmitter in descending pathways emanating from the brain stem; NE-containing fibers terminate in the spinal dorsal cord, particularly in the substantia gelatinosa (SG). NE enhances inhibitory synaptic transmission in SG neurons by activating presynaptic α1-receptors and hyperpolarizes the membranes of SG neurons by acting on α2-receptors; NE may thus act directly on SG neurons of the dorsal spinal cord and inhibit nociceptive transmission at the spinal level. NE-containing fibers also reportedly terminate in lamina X, suggesting that NE also modulates synaptic transmission in lamina X. However, the cellular mechanisms underlying such action have not been investigated. We hypothesized that NE might directly act on lamina X and enhance inhibitory synaptic transmission therein. Using rat spinal cord slices and in vitro whole-cell patch-clamps, we found that the bath-application of NE to lamina X does not affect the excitatory interneurons but enhances GABAergic and glycinergic miniature inhibitory postsynaptic currents (mIPSCs) and induces an outward current. NE-induced enhancement of mIPSCs was blocked by α1A-receptor antagonists, and NE-induced outward current was blocked by α2-receptor antagonists. NE did not affect GABA- or glycine- induced outward currents. These findings are similar to those obtained from SG neurons: NE may act at presynaptic terminals of GABAergic and glycinergic interneurons on lamina X to facilitate inhibitory-transmitter release through α1A-receptor activation and directly induce inhibitory interneuron membrane hyperpolarization through α2-receptors activation.

File： Ohashi(Neurocience,2019).pdf

DOI： 10.1016/j.neuroscience.2019.04.004

PubMed

researchmap

　 PREV - NEXT 　

<BABA Hiroshsi>

Papers - BABA Hiroshsi