研究者詳細 - 大久保　真樹

A multicenter validation of regional cerebral blood flow quantitation using [I-123]iodoamphetamine and single photon emission computed tomography 査読

その他リンク： http://dspace.lib.niigata-u.ac.jp/dspace/handle/10191/33172

I-123 IMPによる新しい脳機能定量法査読

大久保真樹, 小田野行男, 高橋誠他

映像情報メディカル 28 ( 18 ) 1079 - 1083 1996年9月

H Iida, T Akutsu, K Endo, H Fukuda, T Inoue, H Ito, S Koga, A Komatani, Y Kuwabara, T Momose, S Nishizawa, Odano, I, M Ohkubo, Y Sasaki, H Suzuki, B Tanada, H Toyama, Y Yonekura, T Yoshida, K Uemura

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 16 ( 5 ) 781 - 793 1996年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：LIPPINCOTT-RAVEN PUBL

Recently, two methods have been proposed for regional cerebral blood flow (rCBF) quantitation using [I-123]iodoamphetamine (IMP) and single-photon emission computed tomography (SPECT). The table look-up (TLU) method has been shown to provide both rCBF and volume of distribution, V-d, images from two SPECT scans, while a single-scan autoradiographic (ARG) technique provided rCBF using a fixed and assumed V-d. In both methods, a single blood sample was referred to calibrate the previously determined standard input function The present multicenter project was designed to evaluate the accuracy of both methods for use as clinical investigative tools. Ten independent institutions performed [I-123]IMP-SPECT studies according to both methods in 76 subjects (10 normal volunteers, 32 patients with cerebrovascular disease, and 34 patients with other diseases). Calculated rCBF values were compared with those obtained by the following reference methods available in the participating institutions; [O-15] H2O positron emission tomography (PET) (five institutions), [Xe-133]SPECT (four institutions), and the [I-123]IMP microsphere method (three institutions). Both ARG and TLU methods provided rCBF values that were significantly correlated with those measured by the [O-15] H2O PET technique (p < 0.001 for all subjects; overall regression equation, y = 15.14 + 0.54x) and those measured by the [I-123]IMP-microsphere method (p < 0.001 for all subjects; y = 2.0 + 0.80x). Significant correlation (p < 0.05) was observed in 18 of 24 subjects studied with the [Xe-133] SPECT reference technique (overall regression equation, y = 15.0 + 0.55x). Mean cortical gray matter rCBF in a group of normal subject was 43.9 +/- 3.3 and 33.4 +/- 2.0 ml/min/100 g for the ARG and TLU methods, respectively. Regional V-d of [I-123]IMP estimated by the TLU method was 45 ml/ml +/- 20% in the normal cortical region, Close agreement between ARG and TLU rCBF values was observed (y = -3.21 + 1.07x, r = 0.97), confirming the validity of assuming a fixed V-d in the ARG method. Results of this study demonstrate that both the ARG and TLU methods accurately and reliably estimate rCBF in a variety of clinical settings.

DOI： 10.1097/00004647-199609000-00003

123I-IMPの分布容積を用いたSPECTによる脳機能診断査読

小田野行男, 大久保真樹, 高橋誠

核医学 33 ( 6 ) 579 - 586 1996年6月

記述言語：日本語出版者・発行元：(一社)日本核医学会

1)123I-IMPの再分布現象を分布容積という定量可能な変数に変換するMagic square法をプログラム化してSPECT装置に導入し,機能画像として日常診療に応用する方法を開発した. 2)本システムは,一点動脈採血法(マイクロスフェアー法)による脳血流測定と2回のSPECTスキャンにより稼働する. 3)最終的な分布容積像の他に脳血流像,k2像及びdelayed/early ratio像を作成し,計4種の機能画像として作成・表示可能である.又,それらの数値データと標準偏差を得ることができる. 4)分布容積は123I-IMPの脳内保持機能の程度を反映すると考えられるので,この変数を用いた新たな脳機能診断が日常診療として可能になると思われた

その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1996&ichushi_jid=J00251&link_issn=&doc_id=19960702070001&doc_link_id=10008536256&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F10008536256&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

Decrease in benzodiazepine receptor binding in a patient with Angelman syndrome detected by iodine-123 iomazenil and single-photon emission tomography 査読

Odano, I, T Anezaki, M Ohkubo, Y Yonekura, Y Onishi, T Inuzuka, M Takahashi, S Tsuji

EUROPEAN JOURNAL OF NUCLEAR MEDICINE 23 ( 5 ) 598 - 604 1996年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER VERLAG

A receptor mapping technique using iodine 123 iomazenil and single-photon emission tomography (SPET) was employed to examine benzodiazepine receptor binding in a patient with Angelman syndrome (AS). AS is characterized by developmental delay, seizures, inappropriate laughter and ataxic movement. In this entity there is a cytogenic deletion of the proximal long arm of chromosome 15q11-q13, where the gene encoding the GABA(A) receptor beta 3 subunit (GABRB3) is located. Since the benzodiazepine receptor is constructed as a receptor-ionophore complex that contains the GABA(A) receptor, it is a suitable marker for GABA-ergic synapsis. To determine whether benzodiazepine receptor density, which indirectly indicates changes in GABA(A) receptor density, is altered in the brain in patients with AS, we investigated a 27-year-old woman with AS using I-123-iomazenil and SPET. Receptor density was quantitatively assessed by measuring the binding potential using a simplified method. Regional cerebral blood flow was also measured with N-isopropyl-p-[I-123]iodoampheramine. We demonstrated that benzodiazepine receptor density is severely decreased in the cerebellum, and mildly decreased in the frontal and temporal cortices and basal ganglia, a result which is considered to indicate decreased GABA(A) receptor density in these regions. Although the deletion of GABRB3 was not observed in the present study, we indirectly demonstrated the disturbance of inhibitory neurotransmission mediated by the GABA(A) receptor in the investigated patient. I-123-iomazenil with SPET was useful to map benzodiazepine receptors, which indicate GABA(A) receptor distribution and their density.

５-FUおよびCDDPのFM3A細胞の細胞回転に及ぼす影響査読

N-isopropyl-p-[123I]iodoamphetamine (123I-IMP) SPECTによる局所脳血流及び分布容積の新しい定量測定法査読

大久保真樹, 小田野行男, 高橋誠, 他

核医学 33 ( 3 ) 241 - 249 1996年

N-isopropyl-p-[123I]iodoamphetamine (123I-IMP) SPECTによる局所脳血流の定量測定法の比較・検討査読

大久保真樹, 小田野行男, 高橋誠, 他

核医学 33 ( 6 ) 647 - 654 1996年

FM3A細胞に対する５-FU，CDDPおよび放射線の併用効果に関する研究

藤田勝三, 大久保真樹, 酒井邦夫

関越UFT研究会 8 9 - 14 1995年12月

藤田勝三, 大久保真樹, 酒井邦夫, 日向浩

新潟大学医療技術短期大学部紀要 5 ( 3 ) 259 - 263 1995年12月

記述言語：日本語掲載種別：研究論文（大学，研究機関等紀要）出版者・発行元：新潟大学医療技術短期大学部

FM3A細胞に対する5-FUとCDDPの細胞回転に及ぼす影響をフローサイトメトリーにより解析した. 1)5-FU単独処理により,濃度0.1μg/mlでは僅かに早期S期細胞が増加し,0.5μg/ml以上ではG1期からS期への細胞周期の移行が阻害された.CDDP単独処理では,0.1μg/ml以上でG2ブロックが認められた. 2)5-FU(0.1μg/ml),CDDP(0.1μg/ml)それぞれ単独処理では,薬剤除去24時間後のDNAヒストグラムは元のパターンに戻り,部分同調は認められなかった. 3)5-FU処理(0.1μg/ml,24時間)終了直後にCDDP処理を併用した場合,CDDP濃度0.01μg/mlでは細胞回転に大きな変化はなかった.CDDP濃度0.1μg/mlでは18時間後に顕著なS期細胞の増加,24時間後にはS期細胞の減少とG2M期細胞の著しい増加がみられ,明らかな部分同調が認められた

Combined effect of radiation and 5-fluorouracil on survival of FM3A mouse mammary adenocarcinoma cells 査読

Fujita S, Sakai K, Hicughi S, Sueyama H, Ohkubo M

Bull Coll Biomed Technol Niigata Univ 5 ( 3 ) 265 - 269 1995年12月

Can the microsphere model be applied to cerebral blood flow measurement using N-isopropyl-p-[I-123]iodoamphetamine with SPET regardless of washout from brain tissue? 査読

M Ohkubo, Odano, I, N Takahashi, K Sakai

NUCLEAR MEDICINE COMMUNICATIONS 16 ( 12 ) 1026 - 1033 1995年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHAPMAN HALL LTD

The microsphere model and the continuous withdrawal of arterial blood have commonly been used in clinical studies when measuring regional cerebral blood flow (rCBF) by N-isopropyl-p-[I-123]iodoamphetamine (IMP) single photon emission tomography. The method is considered to underestimate rCBF because of the washout of tracer from brain tissue; however, the extent of this underestimation is not known. To assess whether this underestimation can be determined quantitatively, we performed simulation analysis based on the microsphere model and the two-compartment model [influx, K-1(rCBF); and outflux, k(2)(washout)] using the time-activity curves of I-123-IMP in arterial blood [C-a(t)] of 10 subjects. With the microsphere method, rCBF values fell as time post-injection increased. The extent of underestimation of rCBF was 4.7 +/- 0.28% (mean +/- S.D.) at 5 min, 10.2 +/- 0.42% at 10 min and 15.2 +/- 0.55% at 15 min. There was little variation in the extent of underestimation and it was not dependent on the C-a(t) of the subjects. We therefore considered the results to be generally applicable to various studies of the microsphere model. As 4.7% is considered to be negligible in clinical studies, we conclude that the microsphere model can be applied to obtain accurate measurement of rCBF up to 5 min regardless of washout.

123I-IMP SPECTを用いたmicrosphere modelに基づく局所脳血流量測定法(簡便法)の精度の検討査読

大久保真樹, 小田野行男, 高橋直也

核医学 32 ( 12 ) 1323 - 31 1995年12月

Quantitative assessment of cerebral blood flow measurement using the microsphere model with N-isopropyl-p-[123I]-iodoamphetamine SPECT: simulation analysis for the influence of washout from brain tissue 査読

Ohkubo M, Odano I, Takahashi N, Sakai K

Acta Medica et Biologica 43 ( 3 ) 165 - 171 1995年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：新潟大学

The microsphere model and the continuous withdrawal of arterial blood have been commonly used in clinical studies when measuring regional cerebral blood flow (rCBF) by N-isopropyl-p- [^<123>I]iodoamphetamine (IMP) SPECT. The method is considered to underestimate rCBF because of the washout of the tracer from brain tissue with increasing time after injection; however, the extent of this underestimation is not known. To assess whether this underestimation can be quantitatively determined and until how long after injection the microsphere model can be applied to obtain accurate measurements of rCBF, we performed a simulation analysis. Simulation was based on the microsphere model and the 2-compartment model using the standard time-activity course of ^<123>I-IMP in arterial blood (standard input function), the accuracy of which could be validated. The values of rCBF calculated by the microsphere method following the injection of ^<123>I-IMP were compared with those given in the 2-compartment model [influx; K_1(rCBF) and outflux; k_2 (washout)]. As results, with the microsphere method, rCBF values became lower with the time after injection. The rate of underestimation of rCBF was determined to be 4.6% at 5 min, 10.2% at 10 min and 15.1% at 15 min. Since 4.6% is considered negligible in clinical studies, we concluded that the microsphere model could be applied to obtain accurate measurements of rCBF up to approximately 5 min, regardless of washout. The results of this simulation were confirmed clinically in 11 patients with various cerebral diseases.

CiNii Article

CiNii Books

EVALUATION OF CEREBRAL BLOOD-FLOW IN PATIENTS WITH IDIOPATHIC ORTHOSTATIC HYPOTENSION USING TC-99M HMPAO BRAIN SPECT DURING POSTURAL TESTING 査読

ODANO, I, N TAKAHASHI, T HIGUCHI, M OHKUBO, S HAMA, A TSUDA, H OTAKI, E NOGUCHI, M HATANO

CLINICAL NUCLEAR MEDICINE 20 ( 9 ) 807 - 809 1995年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：LIPPINCOTT-RAVEN PUBL

To determine whether regional cerebral blood flow (rCBF) would change on standing in patients with idiopathic orthostatic hypotension (IOH), Tc-99m HMPAO SPECT studies were performed during postural testing in five patients with IOH. After 10 minutes of quiet rest on a bed, the patients arose quickly and, at the same time, the radiotracer was injected intravenously. SPECT data were obtained with a ring-type SPECT scanner. Another dose of Tc-99m HMPAO was injected with the subjects in the supine position, and SPECT was performed again, Image subtraction was used to evaluate the change in rCBF caused by postural testing. In all patients, the authors observed a decrease of rCBF in the frontal cortex and basal ganglia. This preliminary study suggests that changes in rCBF occur In patients with IOH on standing, and Tc-99m HMPAO SPECT performed during postural testing may have an important role in evaluating these changes.

５-FU少量持続静注と放射線の同時併用療法に関する基礎的，臨床的研究査読

動脈一点採血によるN-isopropyl-p-[123I]iodoamphetamineとSPECTを用いた簡便な脳血流測定法－One-point sampling法－査読

小田野行男, 大久保真樹, 高橋直也

核医学 31 ( 10 ) 1219 - 26 1994年10月

酒井邦夫, 末山博男, 小田純一, 樋口正一, 土田恵美子, 杉田公, 松本康男, 藤田勝三, 大久保真樹, 稲越英樹

INNERVISION 9 ( 7 ) 19 - 19 1994年7月

５-FUと放射線の併用効果に関する基礎的研究査読

藤田勝三, 大久保真樹, 酒井邦夫

関越UFT研究会 7 43 - 46 1994年7月

A NEW METHOD OF REGIONAL CEREBRAL BLOOD-FLOW MEASUREMENT USING ONE-POINT ARTERIAL SAMPLING BASED ON THE MICROSPHERE MODEL WITH N-ISOPROPYL-P-[I-123]-IODOAMPHETAMINE SPECT 査読

ODANO, I, M OHKUBO, N TAKAHASHI, T HIGUCHI

NUCLEAR MEDICINE COMMUNICATIONS 15 ( 7 ) 560 - 564 1994年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：CHAPMAN HALL LTD

We developed a new method for quantitative measurement of regional cerebral blood flow (rCBF) using one-point arterial sampling with N-isopropyl-p-[I-123]-iodoamphetamine (I-123-IMP) and single photon emission computed tomography (SPECT) based on the microsphere model. Although the conventional microsphere method requires both the continuous withdrawal of arterial blood (integral of Ca(t)) and treatment of the blood with octanol to obtain the fraction of true tracer activity in the integral of Ca(t) (N), the new method does not require these two procedures. We examined 14 patients to analyse the correlation between the integral of Ca(t)N and a small arterial sample obtained at one time point [one-point Ca(t)] after the injection of I-123-IMp Without octanol treatment. The integral of Ca(t)N was calculated from one point Ca(t) using the regression line of the correlation. An error of 8.1% in the calculated value compared to the actual value of the integral of Ca(t)N, could be inferred from one-point Ca(t) obtained at 6 min after the injection. Then regional cerebral blood flow was measured by the method and a significant correlation was obtained with rCBF measured using the Xe-133 inhalation method (r = 0.773). The one-point Ca(t) method provides fast, easy, accurate and non-invasive measurement of rCBF without inserting catheters and without treatment of arterial blood with octanol.

DOI： 10.1097/00006231-199407000-00011