研究者詳細 - 竹中　彰治

論文 - 竹中　彰治

分割表示 62 件中 41 - 60 件目／全件表示 >>

Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation 査読

Taisuke Hasegawa, Shoji Takenaka, Tatsuya Ohsumi, Takako Ida, Hayato Ohshima, Yutaka Terao, Traithawit Naksagoon, Takeyasu Maeda, Yuichiro Noiri

CLINICAL ORAL INVESTIGATIONS 24 ( 2 ) 963 - 970 2020年2月

Treatment of severe pneumonia by hinokitiol in a murine antimicrobial-resistant pneumococcal pneumonia model. 査読国際誌

Toshihito Isono, Hisanori Domon, Kosuke Nagai, Tomoki Maekawa, Hikaru Tamura, Takumi Hiyoshi, Katsunori Yanagihara, Eiji Kunitomo, Shoji Takenaka, Yuichiro Noiri, Yutaka Terao

PloS one 15 ( 10 ) e0240329 2020年

記述言語：英語掲載種別：研究論文（学術雑誌）

Streptococcus pneumoniae is often isolated from patients with community-acquired pneumonia. Antibiotics are the primary line of treatment for pneumococcal pneumonia; however, rising antimicrobial resistance is becoming more prevalent. Hinokitiol, which is isolated from trees in the cypress family, has been demonstrated to exert antibacterial activity against S. pneumoniae in vitro regardless of antimicrobial resistance. In this study, the efficacy of hinokitiol was investigated in a mouse pneumonia model. Male 8-week-old BALB/c mice were intratracheally infected with S. pneumoniae strains D39 (antimicrobial susceptible) and NU4471 (macrolide resistant). After 1 h, hinokitiol was injected via the tracheal route. Hinokitiol significantly decreased the number of S. pneumoniae in the bronchoalveolar lavage fluid (BALF) and the concentration of pneumococcal DNA in the serum, regardless of whether bacteria were resistant or susceptible to macrolides. In addition, hinokitiol decreased the infiltration of neutrophils in the lungs, as well as the concentration of inflammatory cytokines in the BALF and serum. Repeated hinokitiol injection at 18 h intervals showed downward trend in the number of S. pneumoniae in the BALF and the concentration of S. pneumoniae DNA in the serum with the number of hinokitiol administrations. These findings suggest that hinokitiol reduced bacterial load and suppressed excessive host immune response in the pneumonia mouse model. Accordingly, hinokitiol warrants further exploration as a potential candidate for the treatment of pneumococcal pneumonia.

DOI： 10.1371/journal.pone.0240329

PubMed

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A Horizontal Sequential Cutting Method to Estimate the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy. 査読国際誌

Taisuke Hasegawa, Shoji Takenaka, Rika Wakamatsu, Tatsuya Ohsumi, Hisanori Domon, Hayato Ohshima, Yutaka Terao, Yuichiro Noiri

Journal of endodontics 45 ( 9 ) 1142 - 1147 2019年9月

記述言語：英語掲載種別：研究論文（学術雑誌）

INTRODUCTION: This study aimed to develop a technique to create sequential slices, allowing the fluorescent visualization of bacterial viability in all parts of an infected dentin. METHODS: Cylindrical dentin blocks were prepared from freshly extracted human teeth with a single-rooted canal. Each block was immersed in 5% sodium hypochlorite (NaOCl) and 17% EDTA for 5 minutes before being infected with Enterococcus faecalis. The bacteria were allowed to develop inside dentin specimens for 28 days under anaerobic conditions. The specimens were exposed in 2% NaOCl for either 2 minutes or 20 minutes at 20°C, 37°C, and 45°C, respectively. After staining with calcein AM (Thermo Fisher Scientific, Waltham, MA) and propidium iodide, the samples were cryoembedded, mounted on an adhesive film, and sectioned at a thickness of 10 μm along the running of the dentinal tubules. Stacks of fluorescent images were collected in the z dimension using confocal laser scanning microscopy, and the maximum affected distance from a root canal was measured from the 3-dimensional reconstructed image. The reliability of this technique was verified by comparison with a dye bleaching test. RESULTS: Horizontal sequential sections preserving 3-dimensional bacterial distribution and their viabilities could be made without decalcification. The treatment time contributed to the penetration of NaOCl into dentinal tubules, whereas temperature did not significantly affect the penetration. The judgment by confocal laser scanning microscopic analysis was consistent with that of a dye bleaching test. CONCLUSIONS: The horizontal sectioning method has the advantage of creating sequential sections, allowing information to be imaged at every portion.

DOI： 10.1016/j.joen.2019.06.004

PubMed

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Evidence-based strategy for dental biofilms: Current evidence of mouthwashes on dental biofilm and gingivitis 査読国際誌

Takenaka S, Ohsumi T, Noiri Y

Japanese Dental Science Review 55 ( 1 ) 33 - 40 2019年

The effect of chlorhexidine on dental calculus formation: An in vitro study 査読

Yuuki Sakaue, Shoji Takenaka, Tatsuya Ohsumi, Hisanori Domon, Yutaka Terao, Yuichiro Noiri

BMC Oral Health 18 ( 1 ) 52 2018年3月

Limitations and adverse influences of antimicrobial strategy for the control of oral biofilm. 査読

Takenaka S, Noiri Y

J Dent Oral Biol 2 ( 3 ) 1034 2017年

Vizantin inhibits bacterial adhesion without affecting bacterial growth and causes Streptococcus mutans biofilm to detach by altering its internal architecture 査読

Shoji Takenaka, Masataka Oda, Hisanori Domon, Tatsuya Ohsumi, Yuki Suzuki, Hayato Ohshima, Hirofumi Yamamoto, Yutaka Terao, Yuichiro Noiri

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 480 ( 2 ) 173 - 179 2016年11月

Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans 査読

Yuuki Sakaue, Hisanori Domon, Masataka Oda, Shoji Takenaka, Miwa Kubo, Yoshiyasu Fukuyama, Takashi Okiji, Yutaka Terao

MICROBIOLOGY AND IMMUNOLOGY 60 ( 1 ) 10 - 16 2016年1月

Residual Structure of Streptococcus mutans Biofilm following Complete Disinfection Favors Secondary Bacterial Adhesion and Biofilm Re-Development 査読

Tatsuya Ohsumi, Shoji Takenaka, Rika Wakamatsu, Yuuki Sakaue, Naoki Narisawa, Hidenobu Senpuku, Hayato Ohshima, Yutaka Terao, Takashi Okiji

PLOS ONE 10 ( 1 ) e0116647 2015年1月

Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization 査読

Rika Wakamatsu, Shoji Takenaka, Tatsuya Ohsumi, Yutaka Terao, Hayato Ohshima, Takashi Okiji

CLINICAL ORAL INVESTIGATIONS 18 ( 2 ) 625 - 634 2014年3月

What happens when you rinse? 査読

Takenaka S, Pitts B, Stewart PS

Cell (Journal's online) 2012年

Current and future strategies for the control of mature oral biofilms—Shift from a bacteria-targeting to a matrix-targeting approach. 査読

Takenaka S, Ohshima H, Ohsumi T, Okiji T

J Oral Biosci 54 ( 4 ) 173 - 179 2012年

Translucency and color change of tooth-colored temporary coating materials 査読

Shoji Takenaka, Rika Wakamatsu, Yumiko Ozoe, Fumihito Tomita, Masayoshi Fukushima, Takashi Okiji

AMERICAN JOURNAL OF DENTISTRY 22 ( 6 ) 361 - 365 2009年12月

Diffusion of Macromolecules in Model Oral Biofilms 査読

Shoji Takenaka, Betsey Pitts, Harsh M. Trivedi, Philip S. Stewart

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75 ( 6 ) 1750 - 1753 2009年3月

Impact of Streptococcus mutans on the generation of fluorescence from artificially induced enamel and dentin carious lesions in vitro 査読

Yoshimi Shigetani, Shoji Takenaka, Akira Okamoto, Neamat Abu-Bakr, Masaaki Iwaku, Takashi Okiji

ODONTOLOGY 96 ( 1 ) 21 - 25 2008年7月

Direct visualization of spatial and temporal patterns of antimicrobial action within model oral biofilms 査読

Shoji Takenaka, Harsh M. Trivedi, Audrey Corbin, Betsey Pitts, Philip S. Stewart

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 74 ( 6 ) 1869 - 1875 2008年3月

Removal of resin-based root canal filling materials with K3 rotary instruments: Relative efficacy for different combinations of filling materials 査読

Naoyuki Iizuka, Shoji Takenaka, Yoshimi Shigetani, Takashi Okiji

DENTAL MATERIALS JOURNAL 27 ( 1 ) 75 - 80 2008年1月

Aerotolerance of Peptostreptococcus anaerobius biofilms 査読

Takashi Fukuda, Shoji Takenaka, Kohichi Kota, Masaaki Iwaku, Etsuro Hoshino

Microbial Ecology in Health and Disease 16 ( 4 ) 205 - 210 2004年12月

Bactericidal effects of different root canal cleaning methods on the microroganisms in the deep layers of root canal dentine. 査読

Kota K, Takenaka S, Kashiwada T, Iwaku M

Niigata Dent J 32 ( 2 ) 251 - 255 2002年10月

Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S-mutans biofilm 査読

M Zhu, S Takenaka, M Sato, E Hoshino

ORAL MICROBIOLOGY AND IMMUNOLOGY 16 ( 1 ) 54 - 56 2001年2月

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