研究者詳細 - 寺井　崇二

論文 - 寺井　崇二

分割表示 1,104 件中 1,081 - 1,100 件目／全件表示 >>

HHM: A dominant inhibitory helix-loop-helix protein associated with liver-specific gene expression and liver stem cells 査読

S Terai, SS Thorgeirsson, K Okita

GROWTH, PROLIFERATION, AND APOPTOSIS IN HEPATOCYTES 1 - 9 2002年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：SPRINGER-VERLAG TOKYO

The helix-loop-helix (HLH) family of transcriptional regulatory proteins are key regulators in numerous organ development. We had identified a novel HLH factor, (h) under bar uman (h) under bar omologue of (M) under bar aid (HHM), from a human fetal liver cDNA library. HHM is composed of 360 amino acids (aa). The HHM has an HLH region (150-184 aa) with a leucine zipper motif (240-260 aa) lacking the basic region. The homology of the HLH region for HHM shared 82% identity with that for maternal Id-likemolecule (Maid), but showed low homology with other dominant inhibitory HLH proteins such as Ids, HHM is expressed at a high level in fetal liver as well as in brain, placenta, bone marrow, and lung and is transiently high during liver stem cell activation in the 2-acetylaminofluorene/partial hepatectomy (AAF/PH) model. HHM also inhibited luciferase gene activation induced by the hepatic nuclear factor 4 (HNF-4) promoter. These results suggest that HHM is a novel type of dominant inhibitory HLH protein that might be associated with liver-specific gene expression and liver stem cells.

Web of Science

researchmap

A new cell therapy using bone marrow cells to repair damaged liver 査読

S Terai, N Yamamoto, K Omori, Sakaida, I, K Okita

JOURNAL OF GASTROENTEROLOGY 37 162 - 163 2002年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SPRINGER-VERLAG TOKYO

Hepatic stem cells can transdifferentiate into hepatocytes, bile duct cells, pancreatic cells, or intestinal cells. Hepatic stem cells had been thought to be located in the canal of Hering in the liver, but recent work has demonstrated the existence of hepatic stem cells in bone marrow as well. Cell therapy using autologous bone marrow cells has few ethical problems and many applications for treating severe liver disease. Further in vitro and in vivo analysis is crucial to develop the therapy for clinical use.

Web of Science

PubMed

researchmap

Herbal medicine Inchin-ko-to (TJ-135) prevents liver fibrosis in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet 査読

Sakaida, I, T Yamasaki, S Terai, K Okita

ASIAN PACIFIC ASSOCIATION FOR THE STUDY OF THE LIVER MEETING 2002 153 - 158 2002年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：MEDIMOND S R L

The aim of this study was to investigate whether TJ-135 has any influence on the development of liver fibrosis in rats. The effects of the TJ-135 were examined using the choline-deficient L-amino acid-defined (CDAA) diet-induced liver fibrosis and human hepatic stellate cell line (LI90). TJ-135 prevented fibrosis in a dose-dependent manner up to 1.5% (w/w), as indicated by reduced hydroxyproline content in the liver, without reducing the increase in serum ALT. TJ-135 also reduced the number of activated stellate cells. With LI90 cells, TJ-135 reduced MAP kinase activities resulting in reduced PIIIP concentration in the medium in a dose-dependent manner. These results indicate that TJ-135 significantly reduces liver fibrosis through the inhibition of stellate cell activation.

Web of Science

researchmap

生体部分肝移植で救命し得たB型劇症肝炎急性型の成人例

大森薫, 木村輝昭, 丸本芳雄, 川口浩太郎, 高見太郎, 岡本真理子, 瀬川誠, 坂口栄樹, 岡崎宗子, 寺井崇二

山口医学 50 ( 6 ) 829 - 829 2001年12月

近未来医療移植から再生医療 GFP transgenic mouseを用いた肝臓再生療法の基礎的検討

山本直樹, 寺井崇二, 坂井田功, 大森薫, 川口浩太郎, 高見太郎, 坂口栄樹, 宮本康嗣, 沖田極

肝臓 42 ( Suppl.3 ) A479 - A479 2001年11月

肝細胞癌に対するバルーンマイクロカテーテルを用いた肝動脈バルーン閉塞下ラジオ波凝固療法

山崎隆弘, 黒川典枝, 高見太郎, 山本直樹, 是永匡紹, 木村輝昭, 寺井崇二, 萱野幸三, 坂井田功, 沖田極

肝臓 42 688 - 689 2001年

Human homologue of maid: A dominant inhibitory helix-loop-helix protein associated with liver-specific gene expression 査読

S Terai, H Aoki, K Ashida, SS Thorgeirsson

HEPATOLOGY 32 ( 2 ) 357 - 366 2000年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO

The helix-loop-helix (HLH) family of transcriptional. regulatory proteins are key regulators in numerous developmental processes. The class I HLH proteins, such as E12 are ubiquitously expressed. Class II HLH proteins, such as MyoD, are expressed in a tissue-specific manner, Class I and II heterodimers can bind to E-boxes (CANNTG) and regulate lineage commitments of embryonic cells. In an attempt to identify partners for the E12 protein that may exert control during liver development, we performed the yeast 2-hybrid screen using an expression complementary DNA library from human fetal liver. A novel dominant inhibitory HLH factor, designated HHM (human homologue of maid), was isolated and characterized. HHM is structurally related to the Id family and was highly expressed in brain, pituitary gland, lung, heart, placenta, fetal liver, and bone marrow. HHM physically interacted with E12 in vitro and in mammalian cells. Comparison of the dominant inhibitory effects of HHM and Id2 on the binding of E12/MyoD dimer to an E-box element revealed a weaker inhibition by HHM. However, HHM but not Id2 specifically inhibited the luciferase gene activation induced by hepatic nuclear factor 4 (HNF4) promoter. The HHM was transiently expressed during stem-cell-driven regeneration of the liver at the stage in which the early basophilic foci of hepatocytes started to appear. These results suggest that HHM is a novel type of dominant inhibitory HLH protein that might modulate liver-specific gene expression.

Web of Science

researchmap

Does cytoplasmic cyclin D1 regulate the cell cycle directly? 査読

Terai S, Okita K

J Gastroenterol. 32 ( 2 ) 357 - 66 2000年8月

Antitumor effect of vesnarinone on human hepatocellular carcinoma cell lines 査読

K Kubo, Y Matsuzaki, A Kato, S Terai, K Okita

INTERNATIONAL JOURNAL OF ONCOLOGY 14 ( 1 ) 41 - 46 1999年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PROFESSOR D A SPANDIDOS

Vesnarinone, a quinolinone derivative, induces apoptosis and differentiation in some tumor cell lines. We examined the antitumor effect of vesnarinone on three human hepatocellular carcinoma (HCC) cell lines. Vesnarinone suppressed the proliferation of all three HCC cell lines, but did not induce apoptosis and differentiation. Vesnarinone has also been reported to inhibit both nucleoside and nucleobase transport. Concomitant administration of vesnarinone and a de novo nucleotide synthesis inhibitor potentiated the growth-inhibitory effect of vesnarinone on HCC cells. Vesnarinone may be useful as a new biochemical modulator of anticancer agents.

Web of Science

researchmap

肝細胞癌に対する経皮的熱湯局注療法併用下マイクロ波凝固治療

山崎隆弘, 加藤彰, 草野倫好, 弘中孝治, 久保圭子, 寺井崇二, 黒川典枝, 藪下芳子, 森健治, 水町宗治, 沖田極

Journal of Microwave Surgery 16 37 - 41 1998年

出版者・発行元：Study Group of Microwave Surgery

We compared the usefulness of percutaneous hot water injection and microwave coagulation therapy (PHMCT) and percutaneous microwave coagulation therapy (PMCT) in patients with hepatocellular carcinomas (HCCs). We performed PHMCT in 18 patients with HCCs (23 nodules) measuring less than 30 mm in diameter and PMCT in 5 patients with HCCs (7 nodules).<br>After 1 week of treatment, we performed a dynamic computed tomography (CT) scan to examine the maximum area of necrosis. There was significant difference in long and short axes diameter of the necrotic area between the PHMCT group injected hot water with more than 15 ml and the PMCT group. Our findings indicate that PHMCT is an effective treatment for small HCCs.

DOI： 10.3380/jmicrowavesurg.16.37

CiNii Article

researchmap

その他リンク： http://search.jamas.or.jp/link/ui/1999230833

Inhibition of tumor necrosis factor-alpha production retards liver regeneration after partial hepatectomy in rats 査読

T Kimura, Sakaida, I, S Terai, Y Matsumura, K Uchida, K Okita

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 231 ( 3 ) 557 - 560 1997年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS

We studied the effects of a tumor necrosis factor-alpha (TNF-alpha) production inhibitor E3330, [(2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4-benzoquinolyl)]-2-nonyl-2-propenoic acid] on liver regeneration in rats subjected to 70% partial hepatectomy, The BrdU labeling index was 53.5 +/- 3.8% 24 h after partial hepatectomy alone and was significantly inhibited by E3330 (25 and 50 mg/kg) in a dose-dependent manner (43.3 +/- 2.1% and 19.0 +/- 10.3%, respectively; p<0.05, p<0.01). In these 3 groups, TNF-alpha protein levels in liver tissue were 187 +/- 18, 123 +/- 47, and 89 +/- 33 pg/mg total protein, respectively, with a significant decrease by E3330 administration (p<0.01), TNF-alpha mRNA expression in liver tissue was also reduced by E3330, These results suggest that TNF-alpha promotes liver regeneration after partial hepatectomy. (C) 1997 Academic Press.

Web of Science

researchmap

Wild-type p53 gene-induced morphological changes and growth suppression in hepatoma cells 査読

Shuji Terai, Takafumi Noma, Teruaki Kimura, Atsushi Nakazawa, Fumie Kurokawa, Kiwamu Okita

Journal of Gastroenterology 32 ( 3 ) 330 - 337 1997年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Tokyo

The human hepatocellular carcinoma (HCC) cell line, HLF, expresses only mutant-type p53 (mt-p53), which has an amino acid substitution at the 244th residue from glycine to alanine. HLF cells were transfected with wild-type p53 (wt-p53) cDNA construct pC53-SN3, mt-p53 cDNA construct pC53-SCX [which differs by a single nucleotide, resulting in alanine instead of valine at the 143rd residue in p53 (p53-143)], or pCMV-Neo-Bam, as a control, by a liposome method. After G418 selection, three wt-p53 stable transformants (WT), four mt-p53 transformants (MT), and three control vector transformants (VT) were obtained. We analyzed the cell growth and morphological changes of these transformants under different culture conditions [fetal calf serum (FCS), 10%, 1%, and 0%]. Whereas no difference from control in the growth rate and morphology was observed under the 10% FCS conditions, serum starvation induced remarkable phenotypical changes in all three WTs, but not in the other transformant. Corresponding to these phenotypical changes, the transcriptional activity of wt-p53 was increased more than nine fold. These results indicated that serum starvation would induce wt-p53 biological function, which is tightly linked to morphological changes and growth suppression. To induce these changes, the introduction of the wt-p53 gene itself was not sufficient, and additional triggering, i.e., serum starvation, was indispensable.

DOI： 10.1007/BF02934489

Scopus

PubMed

researchmap

肝細胞癌の門脈1次分枝内腫瘍塞栓(Vp3)に対する経皮的熱湯局注療法の試み

山崎隆弘, 黒川典枝, 加藤彰, 久保圭子, 祐徳浩紀, 寺井崇二, 松崎祐子, 安永満, 沖田極

肝臓 37 ( 9 ) 526 - 527 1996年9月

Clinicopathologic features of early hepatocellular carcinoma 査読

T Yamasaki, F Kurokawa, A Kato, K Irie, K Yutoku, S Terai, Y Matsuzaki, M Yasunaga, K Okita

HEPATO-GASTROENTEROLOGY 43 ( 10 ) 926 - 931 1996年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：H G E UPDATE MEDICAL PUBL LTD.

Background/Aims: This study attempts to clarify the clinicopathologic definition of early hepatocellular carcinomas (HCCs).
Materials and Methods: We evaluated 57 patients, with HCCs less than 3 cm in diameter, in terms of prognosis, incidence of extrahepatic metastasis, and tumor recurrence rate following treatment.
Results: Survival was related to both tumor number and histologic differentiation, but was not related to tumor size. Furthermore, prognosis appeared to depend on the functional reserver of the liver. The incidence of extrahepatic metastasis was related to histologic differentiation. There was no significant difference in the recurrence rates of patients with uninodular tumors in terms of tumor size.
Conclusions: Our findings indicate that early HCCs measure 15 mm or less in diameter, are uninodular, and are histologically well-differentiated. Finally, the functional reserve of the liver will likely be an additional parameter that will further characterize early HCCs.

Web of Science

researchmap

Cell death induced by baicalein in human hepatocellular carcinoma cell lines 査読

Y Matsuzaki, N Kurokawa, S Terai, Y Matsumura, N Kobayashi, K Okita

JAPANESE JOURNAL OF CANCER RESEARCH 87 ( 2 ) 170 - 177 1996年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPANESE CANCER ASSOCIATION

We examined the action of baicalein, a flavonoid contained in the herbal medicine sho-saiko-to (TJ-9), on three cell lines of human hepatocellular carcinoma (HCC). Treatment with baicalein strongly inhibited the activity of topoisomerase II and suppressed the proliferation of all three HCC cell lines. But the mode of cell death induced by baicalein differed according to the cell line. Baicalein induced apoptosis in a concentration-dependent mannner in only one cell line, and an increased concentration of baicalein produced cell death via necrosis in the other two lines, These results suggest that the inhibition of topoisomerase ZI is not by itself sufficient for induction of apoptosis, and that there is a more important mechanism which can account for the difference in susceptibility of cells to apoptosis induced by baicalein.

Web of Science

researchmap

Hepatocyte proliferation induced in rats by lead nitrate is suppressed by several tumor necrosis factor alpha inhibitors 査読

Y Kubo, M Yasunaga, M Masuhara, S Terai, T Nakamura, K Okita

HEPATOLOGY 23 ( 1 ) 104 - 114 1996年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO

Lead nitrate induces liver cell proliferation in rats without accompanying liver cell necrosis. However, the mechanism of this proliferation and its effect on hepatocytes remain unknown. Therefore, we examined the liver and blood level of hepatocyte growth factor and tumor necrosis factor alpha (TNF-alpha) at various intervals to determine whether lead nitrate modifies hepatocyte proliferation by altering the production of these cytokines. We also administered several TNF-alpha inhibitors, dexamethasone, adenosine, (2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4-benzoquinoyl)]-2-nonyl-2-propenoic acid (E3330), and pentoxifylline, to rats to clarify whether pretreatment with these inhibitors suppresses the increase of TNF-alpha messenger RNA (mRNA) in the liver and prevents the hepatocyte proliferation induced by lead nitrate. Hepatocyte proliferation occurred by 24 hours and reached a peak 48 hours after a single intravenous injection of lead nitrate (100 mu/mol/kg). TNF-alpha mRNA expression in the Liver was increased 1, 6, and 12 hours after the injection, whereas no alteration was observed in liver or blood level of hepatocyte growth factor. Pretreatment with dexamethasone (4.0 mg/kg), E3330 (100 mg/kg) adenosine (0.3 mmol/kg), and pentoxifylline (100 mg/kg), inhibited both TNF-alpha mRNA expression and hepatocyte proliferation 48 hours after the injection, These experimental results strongly support the hypothesis that TNF-alpha positively regulates the hepatocyte proliferation induced in rats by the mitogen, lead nitrate.

DOI： 10.1053/jhep.1996.v23.pm0008550029

Web of Science

researchmap

HIGH MDM2 MESSENGER-RNA EXPRESSION IN HEPATOBLASTOMA CELL-LINES 査読

S TERAI, Y MATSUZAKI, M MASUHARA, S KONDOU, M YASUNAGA, K OKITA

INTERNATIONAL HEPATOLOGY COMMUNICATIONS 3 ( 6 ) 330 - 336 1995年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI PUBL IRELAND LTD

Both p53 and MDM2 genes are parts of a physiological pathway frequently impaired in human cancer. This is the report on the analysis of p53 and MDM2 genes in a group of four hepatocellular carcinoma cell-lines and one hepatoblastoma cell-line. Four cell-lines were screened for the presence of p53 mutations using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method. Investigation of the MDM2 gene revealed neither gene amplification nor rearrangement in all cell-lines. However MDM2 mRNA in a hepatoblastoma cell line(HepG2) was elevated to about 2.4 times the level of normal human liver.

Web of Science

researchmap

PERCUTANEOUS HOT-WATER INJECTION THERAPY (PHOT) FOR TREATMENT OF HEPATOCELLULAR-CARCINOMA - A COMPARISION WITH PERCUTANEOUS ETHANOL INJECTION THERAPY (PEIT)

T YAMASAKI, Y MATSUZAKI, K IRIE, S TERAI, A YAMASHITA, F KUROKAWA, M YASUNAGA, K OKITA

INTERNATIONAL HEPATOLOGY COMMUNICATIONS 3 ( 6 ) 305 - 309 1995年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCI PUBL IRELAND LTD

We investigated the safety and efficacy of percutaneous hot water injection therapy (PHoT) in six patients with hepatocellular carcinoma (HCC). PHoT produced the marked effect in five (62.5%) of eight lesions. No serious adverse effects have been encountered. We also compared the usefulness of percutaneous ethanol injection therapy (PEIT) (22 patients, 32 lesions) and PHoT (five patients, seven lesions) in patients with HCC with diameter of 3 cm or less. Patients in the PHoT group received significantly fewer treatments than in the PEIT group (P < 0.01), because the volume injected per treatment was greater. Residual cancer was found in five (15.6%) of 32 lesions after PEIT and in three (42.9%) of seven lesions after PHoT, but the difference was not statistically significant. Pain tended to be less frequent in patients treated with PHoT than in patients treated with PEIT. We could not determine whether PHoT was superior to PEIT, but our findings indicate that PHoT may be a safe and effective for treating HCC.

Web of Science

researchmap

肝細胞癌に対する熱湯局注療法(PHoT)の臨床的検討経皮的エタノール注入療法(PEIT)との比較

山崎隆弘, 松崎祐子, 入江和彦, 寺井崇二, 山下仰, 黒川典枝, 安永満, 沖田極

肝臓 35 ( 7 ) 570 - 571 1994年7月

Endoscopic ligation of gastric varices using a detachable snare 査読

T. Yoshida, N. Hayashi, N. Suzumi, S. Miyazaki, S. Terai, T. Itoh, S. Nishimura, T. Noguchi, K. Hino, M. Yasunaga, M. Tada, K. Okita

Endoscopy 26 ( 5 ) 502 - 505 1994年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Georg Thieme Verlag

A new technique of endoscopic treatment for gastric varices is presented here, which was applied in 10 patients, either electively (n=9) or as emergency therapy for bleeding (n=9) or as emergency therapy for bleeding (n=1). A detachable snare is placed endoscopically, tightened around the varix, and then detached using a similar principle to that of band ligation. Following one or two treatment sessions (one snare applied in seven cases, two snares applied in three cases), eradication of gastric varices was observed in all patients. No significant complications were encountered, and nine of 13 snares passed spontaneously, the remaining ones being removed when found during follow-up endoscopy. Short-term follow-up (4-12 months, mean: 7.2 months) did not show either reappearance of varices or rebleeding in any of the patients. Anigography and endoscopic ultrasonography performed in four patients before and after treatment showed regression or disappearance of intramural vessels. Further studies will show the relative value of this new technique compared to other treatment modalities such as banding or cyanoacrylate injection.

DOI： 10.1055/s-2007-1009013

<寺井 崇二>

論文 - 寺井 崇二

<寺井　崇二>

論文 - 寺井　崇二